Figure 7. Co-treatment with JQ1 and PS synergistically induces apoptosis of MOLM13 cells with and without resistance to FLT3-TKI.
A. MOLM13 and MOLM-TKIR cells were treated with the indicated concentrations of JQ1 for 24 hours. Immunoblot analyses were conducted as indicated. The numbers beneath the blots represent densitometry analysis conducted on representative blots. B. MOLM-TKIR cells were treated with the indicated concentrations of JQ1 and/or PS for 48 hours. The % of annexin V-positive, apoptotic cells was determined by flow cytometry. Columns, mean of two experiments; Bars, S.D. * indicates apoptosis values significantly greater in the combination, compared to treatment with either agent alone (p < 0.05). C. MOLM-TKIR cells were treated with JQ1 and PS, as indicated for 48 hours. Following this, the % of annexin V-positive, apoptotic cells was determined by flow cytometry. Median dose effect and isobologram analysis was performed utilizing Calcusyn. CI values less than 1.0 indicate a synergistic interaction of the two agents in the combination. D. MOLM13-TKIR cells were treated with the indicated concentrations of JQ1 and PS for 24 hours. Cell lysates were prepared and immunoblot analyses were conducted as indicated. The numbers beneath the blots represent densitometry analysis conducted on representative blots.