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WO2010071837A1 - Pyrazine derivatives useful as inhibitors of atr kinase - Google Patents

Pyrazine derivatives useful as inhibitors of atr kinase Download PDF

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Publication number
WO2010071837A1
WO2010071837A1 PCT/US2009/068827 US2009068827W WO2010071837A1 WO 2010071837 A1 WO2010071837 A1 WO 2010071837A1 US 2009068827 W US2009068827 W US 2009068827W WO 2010071837 A1 WO2010071837 A1 WO 2010071837A1
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WO
WIPO (PCT)
Prior art keywords
alkyl
aliphatic
compound
optionally substituted
ring
Prior art date
Application number
PCT/US2009/068827
Other languages
French (fr)
Inventor
Jean-Damien Charrier
Steven Durrant
David Kay
Ronald Knegtel
Somhairle Maccormick
Michael Mortimore
Michael O'donnell
Joanne Pinder
Alistair Rutherford
Anisa Nizarali Virani
Stephen Young
Philip Michael Reaper
Original Assignee
Vertex Pharmaceuticals Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to MX2011006503A priority Critical patent/MX2011006503A/en
Priority to CN201610960262.1A priority patent/CN106518856B/en
Priority to NO09798991A priority patent/NO2376485T3/no
Priority to ES09798991.7T priority patent/ES2663222T3/en
Application filed by Vertex Pharmaceuticals Incorporated filed Critical Vertex Pharmaceuticals Incorporated
Priority to RU2011129761/04A priority patent/RU2604066C2/en
Priority to NZ593969A priority patent/NZ593969A/en
Priority to EP09798991.7A priority patent/EP2376485B1/en
Priority to CA2747252A priority patent/CA2747252C/en
Priority to SI200931816T priority patent/SI2376485T1/en
Priority to CN200980155778.7A priority patent/CN102300862B/en
Priority to KR1020117016218A priority patent/KR101745331B1/en
Priority to KR1020177018220A priority patent/KR101958632B1/en
Priority to AU2009327357A priority patent/AU2009327357C1/en
Priority to SG2011044724A priority patent/SG172248A1/en
Priority to JP2011542506A priority patent/JP5753093B2/en
Priority to PL09798991T priority patent/PL2376485T3/en
Priority to PL17205270T priority patent/PL3354650T3/en
Priority to LTEP09798991.7T priority patent/LT2376485T/en
Priority to EP22156312.5A priority patent/EP4059932A1/en
Priority to EP17205270.6A priority patent/EP3354650B1/en
Priority to BRPI0924084-5A priority patent/BRPI0924084B1/en
Priority to DK09798991.7T priority patent/DK2376485T3/en
Priority to RS20180262A priority patent/RS56995B1/en
Priority to KR1020147035688A priority patent/KR101755216B1/en
Publication of WO2010071837A1 publication Critical patent/WO2010071837A1/en
Priority to IL213613A priority patent/IL213613A/en
Priority to ZA2011/04498A priority patent/ZA201104498B/en
Priority to IL236602A priority patent/IL236602B/en
Priority to AU2016222396A priority patent/AU2016222396B2/en
Priority to AU2018201363A priority patent/AU2018201363C1/en
Priority to CY20181100268T priority patent/CY1120002T1/en
Priority to HRP20180385TT priority patent/HRP20180385T1/en
Priority to IL260158A priority patent/IL260158B/en

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    • C07D241/10—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
    • C07D241/14—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
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    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/4965—Non-condensed pyrazines
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    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
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    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
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    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/6558—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
    • C07F9/65583—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system each of the hetero rings containing nitrogen as ring hetero atom
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10S507/00—Earth boring, well treating, and oil field chemistry
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Definitions

  • ATR ATM and Rad3 related
  • ATM and Rad3 related kinase is a protein kinase involved in cellular responses to DNA damage.
  • ATR kinase acts with ATM ("ataxia telangiectasia mutated") kinase and many other proteins to regulate a cell's response to DNA damage, commonly referred to as the DNA Damage Response ("DDR").
  • DDR DNA Damage Response
  • the DDR stimulates DNA repair, promotes survival and stalls cell cycle progression by activating cell cycle checkpoints, which provide time for repair. Without the DDR, cells are much more sensitive to DNA damage and readily die from DNA lesions induced by endogenous cellular processes such as DNA replication or exogenous DNA damaging agents commonly used in cancer therapy.
  • Healthy cells can rely on a host of different proteins for DNA repair including the DDR kinase ATR. In some cases these proteins can compensate for one another by activating functionally redundant DNA repair processes. On the contrary, many cancer cells harbour defects in some of their DNA repair processes, such as ATM signaling, and therefore display a greater reliance on their remaining intact DNA repair proteins which include ATR. [0003] In addition, many cancer cells express activated oncogenes or lack key tumour suppressors, and this can make these cancer cells prone to dysregulated phases of DNA replication which in turn cause DNA damage. ATR has been implicated as a critical component of the DDR in response to disrupted DNA replication. As a result, these cancer cells are more dependent on ATR activity for survival than healthy cells.
  • ATR inhibitors may be useful for cancer treatment, either used alone or in combination with DNA damaging agents, because they shut down a DNA repair mechanism that is more important for cellular survival in many cancer cells than in healthy normal cells.
  • ATR inhibition has been shown to be effective in cancer cells as single agents and as potent sensitizers to radiotherapy and genotoxic chemotherapy.
  • ATR peptide can be expressed and isolated using a variety of methods known in the literature (see e.g., Unsal-Kacmaz et al, PNAS 99: 10, pp6673-6678, May 14, 2002; see also Kumagai et al. Cell 124, pp943-955, March 10, 2006; Unsal-Kacmaz et al. Molecular and Cellular Biology. Feb 2004, pl292-1300; and Hall-Jackson et al. Oncogene 1999, 18,
  • the present invention relates to pyrazine compounds useful as inhibitors of ATR protein kinase.
  • the invention also relates to pharmaceutically acceptable compositions comprising the compounds of this invention; methods of treating of various diseases, disorders, and conditions using the compounds of this invention; processes for preparing the compounds of this invention; intermediates for the preparation of the compounds of this invention; and methods of using the compounds in in vitro applications, such as the study of kinases in biological and pathological phenomena; the study of intracellular signal transduction pathways mediated by such kinases; and the comparative evaluation of new kinase inhibitors.
  • These compounds have an unexpected ability to treat cancer as single agents. These compounds also show surprising synergy with other cancer agents, such as cisplatin, in combination therapies.
  • One aspect of this invention provides a compound of Formula IA:
  • Y is a C 1- C 1 oaliphatic chain wherein up to three methylene units of the aliphatic chain are optionally replaced with O, NR 0 , S, C(O) or S(O) 2 ;
  • Ring A is a 5 membered heteroaryl ring selected from
  • J 3 is H or C 1-4 alkyl wherein 1 methylene unit of the alkyl group can optionally be replaced with O, NH, N( C 1-4 alkyl), or S and optionally substituted with 1-3 halo;
  • Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 5 is H; a 3-7 membered monocyclic fully saturated, partially unsaturated, or aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; an 8-10 membered bicyclic fully saturated, partially unsaturated, or aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R 5 is optionally substituted with 1-5 J 5 groups;
  • L is a C 1-4 alkyl chain wherein up to two methylene units of the alkyl chain are optionally replaced with O, NR 6 , S, -C(O)-, -SO-, or -SO 2 -;
  • R 0 is H or C 1 -C 6 alkyl wherein one methylene unit of the alkyl chain can be optionally replaced with O, NH, N(d. 4 alkyl), or S;
  • R 1 is H or C 1 -C 6 alkyl
  • R 2 is H, C 1 -C 6 alkyl, -(C 2 -C 6 alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of J z ; or R 1 and R 2 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said heterocyclic ring is optionally substituted with one occurrence of J z ⁇ ;
  • J Z1 is halo, CN, C 1 -C 8 aliphatic, -(X) t -CN, or -(X) r -Z; wherein said up to two methylene units of said C 1 -C 8 aliphatic can be optionally replaced with O, NR, S, P(O), C(O), S(O), or S(O) 2 ; wherein said C 1 -C 8 aliphatic is optionally substituted with halo, CN, Or NO 2 ;
  • X is C 1 -C 4 alkyl; each t, r and m is independently O or 1 ; Z is -NR 3 R 4 ;
  • R 3 is H or C 1 -C 2 alkyl
  • R 4 is H or C 1 -C 6 alkyl; or R 3 and R 4 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said ring is optionally substituted with one occurrence of J z ;
  • R 6 is H, or C 1 -C 6 alkyl
  • J z is independently NH 2 , NH(C 1-4 aliphatic), N(C 1-4 aliphatic) 2 , halogen, C 1-4 aliphatic, OH, O(C 1-4 aliphatic), NO 2 , CN, CO 2 H, CO(C 1-4 aliphatic), CO 2 (C 1-4 aliphatic), O(haloC 1-4 aliphatic), or haloC 1-4 aliphatic;
  • J 5 is halo, oxo, CN, NO 2 , X'-R, Or -(X 1 ) p -Q 4 ;
  • Q 4 is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 8-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q 4 is optionally substituted with 1-5 J Q4 ;
  • J Q4 is halo, CN, or C 1-4 alkyl wherein up to 2 methylene units are optionally replaced with O, NR*, S, C(O), S(O), or S(O) 2 ;
  • R is H or C 1-4 alkyl wherein said C 1-4 alkyl is optionally substituted with 1-4 halo;
  • J 2 is halo; CN; a 5-6 membered aromatic or nonaromatic monocyclic ring having 0-3 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C 1-10 aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O) 2 ; wherein said C 1-10 aliphatic group is optionally substituted with 1-3 halo or CN; and said monocyclic ring is optionally substituted with 1-3 occurences of halo; CN; a C 3 - 6 cycloalkyl; a 3-7 membered heterocyclyl containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C 1-4 alkyl wherein up to one methylene unit of the alkyl chain is optionally replaced with O, NR", or S; and wherein said C 1-4 alkyl is optionally substituted with 1-3 halo; q is 0, 1 , or 2
  • R', R", and R* are each independently H, C 1-4 alkyl, or is absent; wherein said C 1-4 alkyl is optionally substituted with 1-4 halo.
  • Y is a C 1 -C 6 aliphatic chain wherein one methylene unit of the alkyl chain is optionally replaced with C(O) or -NR 0 -;
  • R 5 is a 3-7 membered monocyclic fully saturated, partially unsaturated, or aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic fully saturated, partially unsaturated, or aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R 5 is
  • Ring A is , p is 1 ; and R 5 is aromatic.
  • Ring A is a 5 membered heteroaryl ring selected from
  • ring A is
  • ring A is
  • Ring A structures can be bound to the pyrazine ring in two different ways: as drawn, and the reverse (flipped). For example, when Ring A is it can be bound to the pyrazine ring as shown below:
  • Ring A when Ring A is , it can also be bound to the pyrazine ring in two ways - as drawn and reversed. In some embodiments, the Ring A structures are bound as drawn.
  • J 3 is H.
  • Y is a C 1 ⁇ alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with -NR 0 -.
  • J 5 is a C 1-6 aliphatic group wherein up to 2 methylene units are optionally replaced with O or NR'R" where each R' and R" is independently H or alkyl; or R' and R" taken together to form a 3-6 membered heterocyclic ring; NH 2 ,
  • CO 2 H CO(C 1-4 aliphatic), CO 2 (C 1-4 aliphatic), O(haloC 1-4 aliphatic), or haloC 1 -4aliphatic;
  • J is halo, C 1 -C 2 alkyl optionally substituted with 1-3 fluoro, CN, or a C 1-4 alky! group wherein up to 2 methylene units are optionally replaced with S(O), S(O) 2 , C(O), or NR'.
  • Y is NH;
  • R 5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R 5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R 5 is optionally substituted with 1-5 J 5 groups;
  • L is-C(O>- or -SO 2 -;
  • R 1 is H, or C 1 -C 6 alkyl;
  • R 2 is -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of J z ; or R 1 and R 2 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optional
  • J 5 is halogen, NO 2 , CN, O(haloC
  • J 2 is halo, C 1 -C 2 alkyl optionally substituted with 1-3 fluoro, or CN.
  • L is -C(O)-
  • m is 0, and R 1 and R 2 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms.
  • said heterocyclyl is pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl.
  • m is 0, q is 0, and -L-NR 1 R 2 is C(O)pyrrolidinyl, C(O)piperidinyl, C(O)piperazinyl, C(O) azepanyl, C(O) 1,4-diazepanyl, C(0)NH-piperidinyl, C(0)NHCH 2 CH 2 -pyrrolidinyl, C(0)NHCH 2 CH 2 -piperidinyl, CON(CH 3 )CH 2 CH 2 N(CFb) 2 , wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl or 1 ,4-diazepanyl is optionally substituted with C 1-4 alkyl or N(C 1-4 alkyl) 2 .
  • J 2 is halo; CN; phenyl; oxazolyl; or a C 1-6 aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O) 2 ; said C 1-6 aliphatic group is optionally substituted with 1-3 fluoro or CN.
  • Another embodiment provides a compound of Formula IA': IA' or a pharmaceutically acceptable salt thereof; wherein
  • Y is a C 1- C4alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with -NR 0 -; G is O or S; Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R 5 is a 3-7 membered monocyclic fully saturated, partially unsaturated, or aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic fully saturated, partially unsaturated, or aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R 5 is optionally substituted with 1-5 J 5 groups; L is C 1-4 alkyl chain wherein up to two methylene units of the alkyl chain are optionally replaced with O, NR 6 , S, -C(O)-, -
  • Z is -NR 3 R 4 ;
  • R 3 is H or C 1 -C 2 alkyl
  • R 4 is H or C 1 -C 6 alkyl; or R 3 and R 4 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of J z ;
  • R 6 is H, or C 1 -C 6 alkyl;
  • J z is independently NH 2 , NH(C 1-4 aliphatic), N(C 1-4 aliphatic) 2 , halogen, C 1-4 aliphatic, OH,
  • J 5 is halo, oxo, CN, NO 2 , X'-R, OT -(X 1 ) p -Q 4 ,
  • X 1 is C 1-10 aliphatic; wherein 1-3 methylene units of said C 1-10 aliphatic are optionally replaced with -NR'-, -0-, -S-, C(O), S(O) 2 , or S(O); wherein X 1 is optionally and independently substituted with 1-4 occurrences of NH 2 , NH(C 1-4 aliphatic), N(C 1-4 aliphatic) 2 , halogen, C 1-4 aliphatic, OH, O( C 1-4 aliphatic), NO 2 , CN, CO 2 H, CO 2 ( C 1-4 aliphatic), C(O)NH 2 , C(O)NH(C 1-4 aliphatic), C(O)N(C 1-4 aliphatic) 2 , SO( C 1-4 aliphatic), SO 2 (C 1-4 aliphatic), SO 2 (C 1-4 aliphatic), SO 2 NH(C 1-4 aliphatic), SO 2 NH(C 1-4 ali
  • Q 4 is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 8-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q 4 is optionally substituted with 1-5 J Q4 ;
  • J Q4 is halo, CN, or C 1-4 alkyl wherein up to 2 methylene units are optionally replaced with O, NR*, S, C(O), S(O), or S(O) 2 ;
  • R is H or C 1-4 alkyl wherein said C 1-4 alkyl is optionally substituted with 1-4 halo;
  • J 2 is halo; CN; a 5-6 membered aromatic or nonaromatic monocyclic ring having 0-3 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C 1-10 aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O) 2 ; wherein said C 1-10 aliphatic group is optionally substituted with 1-3 halo or CN; and said monocyclic ring is optionally substituted with 1-3 halo; CN; a C 3-6 cycloalkyl; a 3-7 membered heterocyclyl containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C 1- 4alkyl wherein up to one methylene unit of the alkyl chain is optionally replaced with O, NR", or S; R', R", and R* are each independently H, C 1-4 alky!, or is absent; wherein said C 1-4 alkyl is optionally substitute
  • J 5 is halogen, NO 2 , CN, 0(haloC 1-4 aliphatic), haloC 1- 4aliphatic, or a C 1- 6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'.
  • J 5 is halo, CN, phenyl, oxazolyl, or a C 1-6 aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR', C(O), S, S(O), or S(O) 2 ; said C 1-6 aliphatic group is optionally substituted with 1-3 fluoro or CN.
  • J 2 is halo; CN; phenyl; oxazolyl; or a C 1- 6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O) 2 ; said C 1-6 aliphatic group is optionally substituted with 1-3 fluoro or CN.
  • Y is a C 1 -C 2 alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with NR 0 .
  • p is 0 and Q is phenyl, indolyl, pyridyl, naphthyl or benzothienyl, or quinolinyl.
  • Q is phenyl, indolyl, pyridyl, or quinolinyl.
  • Q is phenyl or pyridyl.
  • phenyl In other embodiments, pyridyl.
  • J 2 is -OCH 3 , -OCH 2 CH 2 N(CH 3 ) 2 , -NHCH 2 CH 2 N(CH 3 ) 2 , or piperazinyl.
  • Q is substituted in the ortho position, the para position, or in both the ortho and the para position.
  • Q is substituted at the para position with J 2 , wherein the J 2 is a C 1-6 aliphatic group wherein the methylene group bonded to Ring Q is replaced with -SO 2 -.
  • the compound of claim 19 wherein at least one more methylene unit of the C 1-6 aliphatic group is optionally replaced with a heteroatom selected from the group consisting of O, NR", and S.
  • Q is optionally substituted in the ortho-position with one
  • J 2 wherein J 2 is C 1- C 4 alkyl, NH 2 , NHC(O)CH 3 , O(C 1- C 4 alkyl), CH 2 OH, CH 2 OCH 3 ,
  • J 2 is substituted in the ortho position with
  • J 2 is CH 2 CH 2 OH, SCH(CH 3 ) 2 , -C ⁇ CCH 3 , halo, CN,
  • Q is optionally substituted in the para position with J 2 , wherein J 2 is -SO 2 (C 1-4 alkyl), -SO 2 (C 3-6 cycloalkyl), -SO 2 (3-6 membered heterocyclyl),
  • said 3-6 membered heterocyclyl is tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, or piperidinyl.
  • Q is optionally substituted in the meta position with J 2 wherein J 2 is C 1-4 alkyl, C 1-4 alkoxy, halo, haloC 1-4 alkyl, haloC 1-4 alkoxyl, CN, SO 2 (C 1-4 aIkyl),
  • Q is naphthyl or benzothienyl.
  • Q is pyridyl.
  • Q is substituted in the ortho-position with one J 2 , wherein J 2 is CN.
  • Q is substituted with one or two J 2 , wherein J 2 is a C 1 - 6 aliphatic group wherein up to 2 methylene units are optionally replaced with O or NR".
  • J 2 is -OCH 3, -OCH 2 CH 2 N(CH 3 ) 2 , -NHCH 2 CH 2 N(CH 3 ) 2 , or piperazinyl.
  • p is 1.
  • Q is phenyl, pyridyl, or naphthyl.
  • said pyridyl is 3-pyridyl or 4-pyridyl.
  • Q is phenyl.
  • Q comprises Q 1 and optionally Q 2 as shown in formula
  • J 5 is halogen, NO 2 , CN, O(haloC 1-4 aliphatic), haloC 1-4 aliphatic, or a C 1-6 aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'.
  • Q 1 is phenyl or pyridyl. In other embodiments, Q'-Q 2 is naphthyl.
  • Y is a C 1 -C 2 alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with NR 0 .
  • L is -C(O)- or -SO 2 -.
  • R 1 and R 2 taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen, sulfur, or oxygen.
  • said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, 1,4-diazepanyl, or 1 ,4-oxazepanyl.
  • R 1 is C 1 -C 6 alkyl.
  • said heterocyclic ring is optionally substituted with halo, CN, C 1 ⁇ aliphatic, haloC 1 - 6 aliphatic, -C(O)O(C 1 - 6 aliphatic),
  • R 2 is C 1 -C 6 alkyl. In other embodiments, R 2 is
  • m is 0.
  • q is 0.
  • L is -C(O)-.
  • R 1 and R 2 taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms.
  • said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl.
  • said heterocyclic ring is selected from
  • t is 1. In other embodments, t is 0.
  • R 1 is H or C 1 -C 6 alkyl; and R 2 is -(C 2 -C 6 alkyl)-Z. In some embodiments, R 1 is C 1 -C 6 alkyl. In some embodiments, Z is -NR 3 R 4 , wherein R 3 and R 4 are both C 1 -C 2 alkyl. In other embodiments, R 3 and R 4 , taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or
  • said ring is pyrrolidinyl or piperidinyl.
  • said ring is optionally substituted with one J Z1 .
  • J Z1 is (X) r -Z.
  • J Z1 is C 1-4 alkyl or N(C 1-4 alkyl) 2 .
  • p is 0, q is 0, and -L-NR 1 R 2 is C(O)pyrrolidinyl,
  • -L-NR 1 R 2 is C(O)1 ,4- di azepanyl.
  • m is 0.
  • R 5 is thienyl, thiazolyl, furanyl, pyrrolidinyl, azetidinyl, piperidinyl, piperazinyl, morpholinyl, pyridinonyl, pyridyl, tetrahydropyridinyl, tetrahydroisoquinolinyl, 1,4-diazepanyl, azabicyclo[2.2.1]heptanyl, or phenyl.
  • R 5 is phenyl, piperidinyl or thienyl.
  • Q is optionally substituted in the para position with -SO 2 (C 1 -
  • R 5 is phenyl.
  • R 5 is optionally substituted with 1-2 J 5 groups, wherein J 5 is selected from halo, CN, NO 2 , X'-R, or -(X') p -Q 4 ; p is 0-1 ;
  • R is H; and
  • Q 4 is a 3-6 membered monocyclic ring containing 0-2 heteroatoms selected from O or N, wherein X 1 is optionally substituted with 1-3 occurrences of halo or CN.
  • J 5 is a C 1-10 aliphatic chain wherein 1-2 methylene units of
  • X 1 are replaced with -O- or -NR'-.
  • R 5 is piperidinyl optionally substituted with NH 2 or
  • R 5 is thienyl optionally substituted with
  • Q 4 is an optionally substituted 3-6 membered cycloalkyl ring. In other embodiments, Q 4 is an optionally substituted 3-6 membered heterocyclic ring selected from pyrrolidinyl, azetidinyl, or thienyl.
  • m is 1.
  • R 5 is H.
  • Y is -NH-, -NHCH 2 -, -NHC(O)-, C(O)NH, C(O)NHCH 2 ,
  • R 5 is phenyl.
  • R 5 is optionally substituted with halo or C 1-4 alkyl, wherein up to 1 methylene unit is optionally replaced with O, NR', or S.
  • Another embodiment provides a compound of Formula HA:
  • Ring A is a 5 membered heteroaryl ring selected from or
  • Y is a C 1- C 4 alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with -NR 0 -;
  • Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
  • R 5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R 5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R 5 is optionally substituted with 1-5 J 5 groups; L iS-C(O)- Or -SO 2 -;
  • R 1 is H, or C 1 -C 6 alkyl;
  • R 0 is H or C 1 -C 6 alkyl;
  • R 2 is C
  • J 5 is halogen, NO 2 , CN, O(haloC 1-4 aliphatic), haloC 1-4 aliphatic, or a C 1-6 aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'; J 2 is halo; CN; phenyl; oxazolyl; or a C 1-6 aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O) 2 ; said C 1-6 aliphatic group is optionally substituted with 1-3 fluoro or CN; R' and R" are each independently H or C 1 -C 4 alkyl; q is O, I, or 2, p is
  • Y is a C 1 -C 2 alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with NR 0 .
  • Another embodiment provides a Compound from Table IIA-2:
  • nother embodiment provides a compound of Formula HIA: IIIA or a pharmaceutically acceptable salt thereof; wherein
  • Y is a C 1- C 4 alkyl chain wherein one methylene unit of the alky I chain is optionally replaced with -NR 0 -;
  • Q is phenyl or pyridyl;
  • R 5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R 5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R is optionally substituted with 1-5 J 5 groups; L iS-C(O)- Or -SO 2 -;
  • R 1 is H, or C 1 -C 6 alkyl;
  • R° is H or C 1 -C 6 alkyl;
  • R 2 is C 1 -C 6 alkyl, -(C 2 -C 6 alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring
  • Y is a C 1 -C 2 alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with NR 0 .
  • p is O and Q is pyridyl.
  • m is O.
  • R 5 is phenyl or thienyl.
  • R 5 is phenyl optionally substituted with one occurrence OfNH 2 , C 1 -C 4 alkyl, or CH 2 NH 2 .
  • Another embodiment provides a compound selected from Table IHA:
  • Y is NH
  • Ring A is a 5 membered heteroaryl ring selected from
  • R 5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R 5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R 5 is optionally substituted with 1 -5 J 5 groups;
  • R 1 is H, or C 1 -C 6 alkyl
  • R " is -(C 2 -C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of
  • J z taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of J Z1 ; J Z1 is -(X)r-CN, C 1 -C 6 alkyl or -(X) 1 -Z; X is C 1 -C 4 alkyl; each t, r and m is independently 0 or 1 ; Z is -NR 3 R 4 ; R 3 is H or C 1 -C 2 alkyl; R 4 is H or C 1 -C 6 alkyl; or R 3 and R 4 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of J z ; each J z , J 1 , and J 5 is independently NH 2 ,
  • Ring A is or
  • m is 0.
  • q is 0.
  • L is -C(O)-.
  • R 1 and R 2 taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms.
  • said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl.
  • said heterocyclic ring is selected from
  • t is 1. In other embodments, t is 0. [0084] in other embodiments, R 1 is H or C 1 -C 6 alkyl; and R 2 is -(C 2 -C6alkyl)-Z. In some embodiments, R 1 is C 1 -C ⁇ alkyl. In some embodiments, Z is -NR 3 R 4 , wherein R 3 and R 4 are both C 1 -C 2 alkyl. In other embodiments, R 3 and R 4 , taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl.
  • said ring is pyrrolidinyl or piperidinyl. [0085] In some embodiments, said ring is optionally substituted with one J Z1 . In some embodiments, J Z1 is (X) r -Z. In other embodiments, J Z1 is C 1-4 alkyl or N(C 1-4 alkyl) 2 .
  • p is 0, q is 0, and -L-NR 1 R 2 is C(O)pyrrolidinyl, C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O) 1,4-diazepanyl, C(O)NH-piperidinyl, C(O)NHCH 2 CH 2 -pyrrolidinyl, C(O)NHCH 2 CH 2 -piperidinyl, CON(CH3)CH 2 CH 2 N(CH3)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl is optionally substituted with C 1-4 alkyl or N(C 1-4 alkyl) 2 .
  • -L-NR 1 R 2 is C(O)1 , 4- diazepanyl.
  • Another embodiment provides a compound of Formula IA-iii: 5 P
  • Ring A is or
  • J 5 O is H, F, Cl, C 1-4 aliphatic, O(C 1-3 aliphatic), or OH;
  • J P is P2 ;
  • J 5 pl is H, C 1-4 aliphatic, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl; wherein J 5 p2 is optionally substituted with 1-2 occurrences of OH or halo; J 5 p2 is H, methyl, ethyl, CH 2 F, CF 3 , or CH 2 OH; J 2 O is H, CN, or SO 2 CH 3 ; J 2 m is H, F, Cl, or methyl; J 2 P is -SO 2 (C 1-6 alkyl), -SO 2 (C 3 - 6 cycloalk;yl), -SO 2 (4-6 membered heterocyclyl),
  • Ring A is N
  • Ring A is N-(2-aminoethyl)-2-aminoethyl-N-(2-aminoethyl)-2-aminoethyl-N-(2-aminoethyl)-2-aminoethyl-N-(2-aminoethyl)-2-aminoethyl-N-(2-aminoethyl)-2-aminoethyl
  • Another embodiments provides a compound from one of the following Tables:
  • Ring A is a 5-6 membered monocyclic aromatic ring containing 0-2 heteroatoms selected from N, O, or S; Ring A is optionally fused to a 5-6 membered aromatic ring containing
  • L is -C(O)-;
  • R 1 is C 1 -Cealkyl;
  • R 2 is - ⁇ C 2 -C6alkyl)-Z or a 4-8 membered heterocyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of J z ; or R 1 and R 2 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of J Z1 ;
  • J Z1 is -(X) 1 -CN, C 1 -C 6 alkyl or -(X) 1 -Z;
  • Z is -NR 3 R 4 ;
  • R 3 is H or C
  • J 2 is halo, C 1 -C 2 alkyl optionally substituted with 1-3 fluoro, or CN; q is O, I , or 2.
  • Ring A is a 6-membered ring not fused to another ring.
  • Ring A is phenyl, pyridyl, or pyrimidyl.
  • Ring A is phenyl.
  • R 1 and R 2 taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms.
  • said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl.
  • said heterocyclic ring is selected from
  • the ring formed by R 1 and R 2 is optionally substituted with CH 2 pyrrolidinyl, C 1-4 alkyl, N(C 1-4 alkyl) 2 , or CH 2 CH 2 CN.
  • t is 1. In other embodiments, t is 0.
  • R 1 is H or C 1 -C 6 alkyl; and R 2 is -(C 2 -C 6 alkyl)-Z.
  • Z is -NR 3 R 4 , wherein R 3 and R 4 are both C 1 -C 2 alkyl.
  • R 3 and R 4 taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl.
  • said ring is pyrrolidinyl or piperidinyl.
  • said ring is optionally substituted with one J z .
  • J z is C 1-4 alkyl or N(C 1-4 alkyl) 2 .
  • p is 0, q is 0, and -L-NR 1 R 2 is C(0)pyrrolidinyl,
  • Another embodiment provides a compound selected from Table II.
  • L is -C(O)- or -SO 2 -;
  • R' is H, or C 1 -C 6 alkyl
  • R 2 is -(C 2 -C6alkyl)-Z or a 4-8 membered heterocyclic ring containing 0-2 nitrogen atoms;,' wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of J z ; or R 1 and R 2 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of J z ⁇ ;
  • J Z1 is -(X) 1 -CN, C 1 -C 6 alkyl or -(X) 1 -Z;
  • X is C 1 -C 4 alkyl; each t, p, and r is independently 0 or 1 ;
  • Z is -NR 3 R 4 ;
  • R 3 is H or C 1 -C 2 alkyl
  • R 4 is H or C 1 -C 6 alkyI; or R 3 and R 4 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of J z ; each J z and J 1 is independently NH 2 , NH(C 1-4 aliphatic), N(C 1-4 aliphatic) 2 , halogen, C 1-4 aliphatic, OH, O(C 1-4 aliphatic), NO 2 , CN, CO 2 H, CO(C 1-4 aliphatic),
  • CO 2 (C 1- 4aliphatic), O(haloC 1 -4aliphatic), or haloC 1 -4 aliphatic;
  • J 2 is halo, C 1 -C 2 alkyl optionally substituted with 1-3 fluoro, or CN;
  • q is O, I, or 2.
  • J 1 is J la or J lb as depicted in Formula Ill-i.
  • L is -C(OK ⁇ is 0, J la is H, and J l b is H or F; then: when R 1 is H; then R 2 is not -(C 1-4 alkyl)-N(CH 3 )2; or
  • R 1 and R 2 taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms.
  • said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl.
  • said heterocyclic ring is selected from
  • t is 1. In other embodments, t is 0. [00108] In other embodiments, R 1 is H or C 1 -C 6 alkyl; and R 2 is -(C 2 -C 6 alkyl)-Z. In some embodiments, Z is -NR 3 R 4 , wherein R 3 and R 4 are both C 1 -C 2 alkyl. In other embodiments, R 3 and R 4 , taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl. In some embodiments, said ring is pyrrolidinyl or piperidinyl.
  • said ring is optionally substituted with one J z .
  • J z is C 1- 4alkyl or N(C 1- 4alkyl) 2 .
  • p is 0, q is 0, and -L-NR 1 R 2 is C(O)pyrrolidinyl, C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O)1, 4-diazepanyl, C(O)NH-piperidinyl, C(O)NHCH 2 CH 2 -pyrrolidinyl, C(O)NHCH 2 CH 2 -piperidinyl, CON(CH3)CH 2 CH 2 N(CH3)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl is optionally substituted with C 1-4 alkyl or N(C 1 -4alkyl) 2 .
  • Another embodiment provides a compound selected from Table III:
  • Ring A is a 8-9 membered bicyclic heteroaryl ring having 1-4 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur;
  • Q is a 5-6 membered monocyclic aromatic ring containingO-3 heteroatoms independently selected from the group consisting of nitrogen, oxygen, and sulfur;
  • L is C 1-4 alkyl chain wherein up to two methylene units of the alkyl chain are optionally replaced with O, NR 6 , S, -C(O)-, -SO-, or -SO 2 -;
  • R' is H or C 1 -C 6 alkyl;
  • R 2 is H, C 1 -C 6 alkyl, -(C 2 -C 6 alkyl)-Z, or a 3-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of J z ; or R 1 and R 2 , taken together
  • X is C 1-4 alkyl; Z is -NR 3 R 4 ; R 3 is H or C 1 -C 2 alkyl; R 4 is H or C 1 -C 6 alkyl; or R 3 and R 4 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of J z ; Z 1 is -NR 5 R 6 ; R 5 is H or C 1 -C 2 alkyl; R 6 is H or C 1 -C 6 alkyl; or R 5 and R 6 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of J Z1 ; J 1 is halo, CN, or a C 1-6 aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR
  • CO 2 (C 1-6 aliphatic), O(haloC 1-4 aliphatic), or haloC 1-4 aliphatic; each q and m is independently O, 1 , or 2; each t, p, and r is independently O or 1.
  • Ring A is a 9-membered ring.
  • Ring A is a 5-6 bicyclic ring system.
  • a 5-6 bicyclic system is a five- membered ring fused to a six membered ring as shown below.
  • Examples of 5-6 bicyclic systems include, but are not limited to, benzimidazolyl, benzoxazolyl, indazolyl, pyrrolopyridinyl, pyrrolopyrimidinyl, pyrrolopyrazinyl, benzothiazolyl, benzothiophenyl, indolyl, benzofuranyl, benzotriazolyl, and azaindolyl.
  • Ring A has 1-2 heteroatoms.
  • Ring A is benzimidazolyl, benzoxazolyl, indazolyl, benzothiazolyl, indolyl, benzotriazolyl, or azaindolyl.
  • Ring Q is phenyl or pyridyl. In some embodiment, Q is phenyl.
  • p is 1 and Ring Q is substituted in the para position with
  • L is C(O) or S(O) 2 .
  • R 1 and R 2 are both C 1 -4alkyl.
  • R 1 and R 2 taken together with the atom to which they are bound, form a 4-7 membered heterocyclic ring containing 1-2 nitrogen atoms.
  • said heterocyclic ring is selected from the group consisting of pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, and 1 ,4-diazepanyl.
  • said heterocyclyl is 1,4-diazepanyl.
  • Ring Q is pyridyl.
  • p is 0. In other embodiments, q is 1 and J 2 is CN. [00122] Another embodiment provides a compound selected from Table V:
  • Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; L iS -C(O)- Or -SO 2 -; G is S or O; R 1 is H, or C 1 -C 6 alkyl; R 2 is - ⁇ C 2 -C 6 alkyl)-Z or a 4-8 membered heterocyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of J z ; or R 1 and R 2 , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of J Z1 ; J Z
  • p is 1.
  • Q is phenyl.
  • L is -C(O)-.
  • R 1 and R 2 taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1 -2 nitrogen atoms.
  • the heterocyclic ring formed by R 1 and R 2 is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl.
  • said heterocyclyl is
  • p is 0, q is 0, and -L-NR 1 R 2 is
  • Another embodiment provides a compound selected from Table VI: Table VI
  • Another embodiment provides a compound of formula VII,
  • Ring A is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said ring is optionally substituted with J 1 ;
  • R 1 is C 1 -C 6 alkyl;
  • J 1 is a C 1 - 6 alkyl chain wherein 1-2 methylene units are optionally replaced with O, NR*, S, or C(O);
  • J 1 is optionally substituted with 1-3 occurrences of halo;
  • R* is H or C 1-4 alkyl;
  • JJ 22 iiss hhaalloo,, CC,i--CC 22 aallkkyyll optionally substituted with 1-3 fluoro, or CN;
  • Each p and q is independently 0, 1 , or 2.
  • Ring A is a 5-6 membered heteroaryl having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
  • Ring A is pyridinyl, pyrimidyl, pyrazinyl, triazinyl, pyrrolyl, pyrazolyl, triazolyl, thienyl, thiazolyl, thiadiazolyl, furanyl, oxazolyl, or oxadiaozolyl.
  • Ring A is pyridinyl, pyrazolyl, thiadiazolyl, or thiazolyl wherein Ring A is optionally substituted with halo or C 1-4 alkyl.
  • Ring A is phenyl. In some embodiments, said phenyl is substituted with one occurrence of J 1 .
  • J 1 is a C 1 - 6 alkyl chain wherein 1 methylene unit is replaced with N or O. In other embodiments, J 1 is O(C 1-4 alkyl) or -(C 1-4 aIkyl)NH(C 1-4 alkyl). In yet other embodiments, J 1 is -(C 1-4 alkyl)NH(C 1-4 alkyl).
  • Another embodiment provides a compound selected from the following:
  • Another embodiment provides a compound selected from Table 1.
  • the variables are as depicted in the compounds of the disclosure including compounds in the tables above.
  • a specified number range of atoms includes any integer therein.
  • a group having from 1-4 atoms could have 1, 2, 3, or 4 atoms.
  • compounds of the invention may optionally be substituted with one or more substituents, such as are illustrated generally herein, or as exemplified by particular classes, subclasses, and species of the invention. It will be appreciated that the phrase “optionally substituted” is used interchangeably with the phrase “substituted or unsubstituted.” In general, the term “substituted”, whether preceded by the term “optionally” or not, refers to the replacement of hydrogen radicals in a given structure with the radical of a specified substituent.
  • an optionally substituted group may have a substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position.
  • Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds.
  • a substituent connected by a bond drawn from the center of a ring means that the substituent can be bonded to any position in the ring.
  • J 1 can be bonded to any position on the pyridyl ring.
  • a bond drawn through both rings indicates that the substituent can be bonded from any position of the bicyclic ring.
  • J 1 can be bonded to the 5-membered ring (on the nitrogen atom, for instance), and to the 6-membered ring.
  • stable refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, recovery, purification, and use for one or more of the purposes disclosed herein.
  • a stable compound or chemically feasible compound is one that is not substantially altered when kept at a temperature of 40°C or less, in the absence of moisture or other chemically reactive conditions, for at least a week.
  • aliphatic or "aliphatic group”, as used herein, means a straight-chain (i.e., unbranched), branched, or cyclic, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation that has a single point of attachment to the rest of the molecule.
  • aliphatic groups contain 1-20 aliphatic carbon atoms. In some embodiments, aliphatic groups contain 1-10 aliphatic carbon atoms. In other embodiments, aliphatic groups contain 1-8 aliphatic carbon atoms. In still other embodiments, aliphatic groups contain 1-6 aliphatic carbon atoms, and in yet other embodiments aliphatic groups contain 1-4 aliphatic carbon atoms. Aliphatic groups may be linear or branched, substituted or unsubstituted alkyl, alkenyl, or alkynyl groups.
  • Specific examples include, but are not limited to, methyl, ethyl, isopropyl, n-propyl, sec-butyl, vinyl, n-butenyl, ethynyl, and tert-butyl.
  • cycloaliphatic refers to a monocyclic C 3 -C 8 hydrocarbon or bicyclic Cs-Cn hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule wherein any individual ring in said bicyclic ring system has 3-7 members.
  • cycloaliphatic groups include, but are not limited to, cycloalkyl and cycloalkenyl groups. Specific examples include, but are not limited to, cyclohexyl, cyclopropenyl, and cyclobutyl.
  • heterocycle means non-aromatic, monocyclic, bicyclic, or tricyclic ring systems in which one or more ring members are an independently selected heteroatom.
  • the "heterocycle”, “heterocyclyl”, or “heterocyclic” group has three to fourteen ring members in which one or more ring members is a heteroatom independently selected from oxygen, sulfur, nitrogen, or phosphorus, and each ring in the system contains 3 to 7 ring members.
  • heterocycles include, but are not limited to, 3-l H-benzimidazol-2- one, 3-(l-alkyl)-benzimidazol-2-one, 2-tetrahydrofuranyl, 3-tetrahydrofuranyl, 2- tetrahydrothiophenyl, 3-tetrahydrothiophenyl, 2-morpholino, 3-morpholino, 4-morpholino, 2- thiomorpholino, 3-thiomorpholino, 4-thiomorpholino, 1-pyrrolidinyl, 2-pyrrolidinyl, 3- pyrrolidinyl, 1-tetrahydropiperazinyl, 2-tetrahydropiperazinyl, 3-tetrahydropiperazinyl, 1- piperidinyl, 2-piperidinyl, 3-piperidinyl, 1-pyrazolinyl, 3-pyrazolinyl, 4-pyrazolinyl, 5- pyrazolinyl, 1-piperidinyl, 2-piperid
  • Cyclic groups (e.g. cycloaliphatic and heterocycles), can be linearly fused, bridged, or spirocyclic.
  • heteroatom means one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro-2H-pyrrolyl), NH (as in pyrrolidinyl) or NR + (as in N-substituted pyrrolidinyl)).
  • unsaturated means that a moiety has one or more units of unsaturation.
  • unsaturated groups can be partially unsaturated or fully unsaturated. Examples of partially unsaturated groups include, but are not limited to, butene, cyclohexene, and tetrahydropyridine.
  • Fully unsaturated groups can be aromatic, anti-aromatic, or non-aromatic. Examples of fully unsaturated groups include, but are not limited to, phenyl, cyclooctatetraene, pyridyl, thienyl, and 1- methylpyridin-2(l H)-one.
  • alkoxy refers to an alkyl group, as previously defined, attached through an oxygen (“alkoxy") or sulfur (“thioalkyl”) atom.
  • haloalkyl means alkyl, alkenyl or alkoxy, as the case may be, substituted with one or more halogen atoms. This term includes perfluorinated alkyl groups, such as -CF3 and -CF 2 CF3.
  • halogen means F, Cl, Br, or I.
  • aryl used alone or as part of a larger moiety as in “aralkyl”, “aralkoxy”, or “aryloxyalkyl”, refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic and wherein each ring in the system contains 3 to 7 ring members.
  • aryl may be used interchangeably with the term “aryl ring”.
  • heteroaryl used alone or as part of a larger moiety as in “heteroaralkyl” or “heteroarylalkoxy”, refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic, at least one ring in the system contains one or more heteroatoms, and wherein each ring in the system contains 3 to 7 ring members.
  • heteroaryl may be used interchangeably with the term “heteroaryl ring” or the term “heteroaromatic”.
  • heteroaryl rings include, but are not limited to, 2-furanyl, 3-furanyl, N- imidazolyl, 2-imidazolyl, 4-imidazolyl, 5-imidazolyl, benzimidazolyl, 3-isoxazolyl, 4- isoxazolyl, 5-isoxazolyl, 2-oxazolyl, 4-oxazolyl, 5-oxazolyl, N-pyrrolyl, 2-pyrrolyl, 3- pyrrolyl, 2-pyridyl, 3-pyridyl, 4-pyridyl, 2-pyrimidinyl, 4-pyrimidinyl, 5-pyrimidinyl, pyridazinyl (e.g., 3-pyridazinyl), 2-thiazolyl, 4-thiazolyl, 5-thiazolyl, tetrazolyl (e.g., 5- tetrazolyl), triazolyl (e.g., 2-triazolyl and 5-triazolyl), 2-thiazo
  • heteroaryl includes certain types of heteroaryl rings that exist in equilibrium between two different forms. More specifically, for example, species such hydropyridine and pyridinone (and likewise hydroxypyrimidine and pyrimidinone) are meant to be encompassed within the definition of "heteroaryl.”
  • a protecting group and “protective group” as used herein, are interchangeable and refer to an agent used to temporarily block one or more desired functional groups in a compound with multiple reactive sites.
  • a protecting group has one or more, or preferably all, of the following characteristics: a) is added selectively to a functional group in good yield to give a protected substrate that is b) stable to reactions occurring at one or more of the other reactive sites; and c) is selectively removable in good yield by reagents that do not attack the regenerated, deprotected ; functional group. As would be understood by one skilled in the art, in some cases, the reagents do not attack other reactive groups in the compound.
  • the reagents may also react with other reactive groups in the compound.
  • protecting groups are detailed in Greene, T. W., Wuts, P. G in "Protective Groups in Organic Synthesis", Third Edition, John Wiley & Sons, New York: 1999 (and other editions of the book), the entire contents of which are hereby incorporated by reference.
  • nitrogen protecting group refers to an agent used to temporarily block one or more desired nitrogen reactive sites in a multifunctional compound.
  • Preferred nitrogen protecting groups also possess the characteristics exemplified for a protecting group above, and certain exemplary nitrogen protecting groups are also detailed in Chapter 7 in Greene, T. W., Wuts, P.
  • a methylene unit of an alkyl or aliphatic chain is optionally replaced with another atom or group.
  • an optional replacement nitrogen atom in this case
  • an optional replacement can be bonded to the aliphatic group via a triple bond.
  • the terminal nitrogen is not bonded to another atom.
  • the term "methylene unit" can also refer to branched or substituted methylene units. For example, in an isopropyl moiety [-CH(CH 3 ) 2 ], a nitrogen atom (e.g.
  • the optional replacements form a chemically stable compound.
  • Optional replacements can occur both within the chain and/or at either end of the chain; i.e. both at the point of attachment and/or also at the terminal end.
  • Two optional replacements can also be adjacent to each other within a chain so long as it results in a chemically stable compound.
  • the optional replacements can also completely replace all of the carbon atoms in a chain.
  • a C3 aliphatic can be optionally replaced by -NR-, -C(O)-, and -NR- to form -NRC(O)NR- (a urea).
  • the replacement atom is bound to a hydrogen atom on the terminal end.
  • the resulting compound could be -OCH 2 CH 3 , -CH 2 OCH 3 , or -CH 2 CH 2 OH.
  • structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, geometric, conformational, and rotational) forms of the structure.
  • isomeric e.g., enantiomeric, diastereomeric, geometric, conformational, and rotational
  • the R and S configurations for each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers are included in this invention.
  • a substituent can freely rotate
  • structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms.
  • compounds having the present structures except for the replacement of hydrogen by deuterium or tritium, or the replacement of a carbon by a 13 C- or 14 C-enriched carbon are within the scope of this invention.
  • Such compounds are useful, for example, as analytical tools or probes in biological assays.
  • the compounds of this invention can exist in free form for treatment, or where appropriate, as a pharmaceutically acceptable salt.
  • a "pharmaceutically acceptable salt” means any non-toxic salt of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof.
  • the term “inhibitorily active metabolite or residue thereof means that a metabolite or residue thereof is also an inhibitor of the ATR protein kinase.
  • Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge et al, describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference.
  • Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. These salts can be prepared in situ during the final isolation and purification of the compounds. Acid addition salts can be prepared by 1) reacting the purified compound in its free-based form with a suitable organic or inorganic acid and 2) isolating the salt thus formed.
  • Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
  • inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid
  • organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange.
  • salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, glycolate, gluconate, glycolate, hemisulfate, heptanoate, hexanoate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, ox
  • Base addition salts can be prepared by 1) reacting the purified compound in its acid form with a suitable organic or inorganic base and 2) isolating the salt thus formed.
  • Salts derived from appropriate bases include alkali metal (e.g., sodium, lithium, and potassium), alkaline earth metal (e.g., magnesium and calcium), ammonium and N + (C 1- 4 alkyl) 4 salts.
  • alkali metal e.g., sodium, lithium, and potassium
  • alkaline earth metal e.g., magnesium and calcium
  • ammonium and N + (C 1- 4 alkyl) 4 salts e.g., sodium, lithium, and potassium
  • alkaline earth metal e.g., magnesium and calcium
  • ammonium and N + (C 1- 4 alkyl) 4 salts e.g., sodium, lithium, and potassium
  • alkaline earth metal e.g., magnesium and calcium
  • ammonium and N + (C 1- 4 alkyl) 4 salts e.g., sodium
  • Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate.
  • Other acids and bases while not in themselves pharmaceutically acceptable, may be employed in the preparation of salts useful as intermediates in obtaining the compounds of the invention and their pharmaceutically acceptable acid or base addition salts.
  • One aspect of this invention provides a compound for use in inhibiting ATR kinase. These compounds have formula I:
  • R 1 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R 1 is optionally substituted with 1-5 J 1 groups;
  • R 2 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R 2 is optionally substituted with 1-5 J 2 groups;
  • L is -C(O)NH- or - €(O)N(C 1-6 alkyl)-; n is 0 or 1 ;
  • Each J 1 and J 2 is independently halo, -CN, -NO 2 , -V'-R, or -(V 2 ) m -Q;
  • V 1 is a C 1-10 aliphatic chain wherein 0-3 methylene units are optionally and independently replaced with O, NR", S, C(O), S(O), or S(O) 2 ; V 1 is optionally substituted with 1-6 occurrences of J v ⁇ ;
  • V 2 is a C 1-10 aliphatic chain wherein 0-3 methylene units are optionally and independently replaced with O, NR", S, C(O), S(O), or S(O) 2 ; V 2 is optionally substituted with 1-6 occurrences or J ; m is O or 1 ;
  • each Q is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 9-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q is optionally substituted with 0-5 J Q ; each J V1 or J V2 is independently halogen, CN, NH 2 , NO 2 , C 1-4 aliphatic, NH(C 1 -4 aliphatic), N(C 1 -4 aliphatic) 2 , OH, O(C 1-4 aliphatic), CO 2 H, CO 2 (C 1-4 aliphatic), C(O)NH 2 , C(O)NH(C i .
  • R is H or C 1- 6aliphatic wherein said C 1-6 aliphatic is optionally substituted with 1-4 occurrences OfNH 2 , NH(C 1-4 aliphatic), N(C 1-4 aliphatic) 2 , halogen, C 1-4 aliphatic, OH, O(C 1-4 aliphatic), NO 2 , CN, CO 2 H, CO 2 (C 1-4 aliphatic), CO(C 1-4 aliphatic), O(haloC 1-4 aliphatic), or haloC 1-4 aliphatic; each J Q is independently halo, oxo, CN, NO 2 , X-R, or -(X) P -Q 4 ; p is O or 1 ;
  • X is C 1-10 aliphatic; wherein 1-3 methylene units of said C 1-6 aliphatic are optionally replaced with -NR, -0-, -S-, C(O), S(O) 2 , or S(O); wherein X is optionally and independently substituted with 1-4 occurrences OfNH 2 , NH(C 1-4 aliphatic), N(C 1-4 aliphatic) 2 , halogen, C 1-4 aliphatic, OH, O(C 1-4 aliphatic), NO 2 , CN, CO(C 1-4 aliphatic), CO 2 H, CO 2 (C 1-4 aliphatic), C(O)NH 2 , C(O)NH(C 1-4 aliphatic), C(O)N(C 1-4 aliphatic) 2 , SO(C 1 -4 aliphatic), SO 2 (C 1-4 aliphatic), SO 2 NH(C i.
  • Q 4 is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 8-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q 4 is optionally substituted with 1-5 J Q4 ;
  • J Q4 is halo, CN, or C 1-4 alkyl wherein up to 2 methylene units are optionally replaced with O, NR*, S, C(O), S(O), or S(O) 2 ;
  • R is H or C 1-4 alkyl wherein said C 1-4 alkyl is optionally substituted with 1-4 halo;
  • R', R", and R* are each independently H, C 1-4 alkyl, or is absent; wherein said C 1-4 alkyl is optionally substituted with 1-4 halo.
  • R 1 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R 1 is optionally substituted with 1-5 J 1 groups;
  • R 2 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R 2 is optionally substituted with 1-5 J 2 groups; each J V1 or J V2 is independently NH2, NH(C 1 -4a!iphatic), N(C 1 -4aliphatic) 2 , halogen, C 1-4 aliphatic, OH, O(C 1-4 aliphatic), NO 2 , CN, CO 2 H, CO 2 (C 1-4 aliphatic), C0(C 1- 4aliphatic), O(haloC 1-4 aliphatic), or haloC 1-4 aliphatic; each J Q is independently halogen, NO 2 , CN, or C
  • R 2 is substituted with one or two occurrences of J 2 .
  • R 2 is substituted with one or two occurrences of J 2 .
  • R 2 is a 5-6 membered monocyclic aromatic ring. In some embodiments, R 2 is a 6-membered aromatic ring. In other embodiments, R 2 is phenyl or pyridyl. In yet other embodiments, R 2 is phenyl.
  • R 2 is optionally substituted with 1-5 J 2 groups. In some embodiments, 1-3 J 2 groups, and in other embodiments, 1-2 J 2 groups. In yet another embodiment, R 2 is substituted with O or 1 occurrences of J 2 .
  • J 2 is -(V 2 ) m -Q or V'-R; wherein each V 1 and V 2 is independently a C 1-6 aliphatic chain wherein 0-3 methylene units are optionally replaced with O, NR', S, C(O), S(O), or S(O) 2 ; wherein the first or second methylene group away from the point of attachment is replaced with C(O), S(O), or S(O) 2 , S or O; m is 1; R is H; and
  • Q is a 5-7 membered monocyclic ring containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said Q is optionally substituted with 1-3 occurrences of halogen, C 1-3 alkyl, CN, OH, O(C 1-3 alkyl), NH 2 , NH(d. 3 alkyl), N(C 1-3 alkyl) 2 , or CO(C 1-3 alkyl).
  • Q is a 5-6 membered monocyclic ring.
  • n is O.
  • R 1 is a 5-6 membered monocyclic aromatic ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 9-10 membered bicyclic aromatic ring having 1-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein each R 1 is optionally substituted with 1-5 J 1 groups.
  • R 1 is benzothiazole, oxadiazole, benzoxazole, triazole, thiadiazole, or isoxazole.
  • R 1 is benzimidazole, benzothioazole, oxadiazole, isoxazole, or triazole. In some embodiments, R 1 is benzimidazole. In other embodiments, R 1 is isoxazole.
  • R 1 is optionally substituted with 1-5 J 1 groups. In some embodiments, 1-3 J 1 groups, and in other embodiments, 1-2 J 1 groups. In some embodiments, J 1 is halo, CN, NO 2 , or -V'-R. In other embodiments, J 1 is halo, CN, C 1-6 alkyl, OR, SR, NR"R, C(O)R, C(O)OR, C(O)NR"R, S(O) 2 R, or S(O)R. In yet other embodiments, J 1 is phenyl optionally substituted with NH 2 , C 1-4 alkyl, thiophene, or CH 2 NH 2 .
  • R 2 is a 5-6 membered monocyclic aromatic ring. In some embodiments, R 2 is a 6-membered aromatic ring. In other embodiments, R 2 is phenyl or pyridyl. In yet other embodiments, R 2 is phenyl.
  • J 2 is -V'-R or - ⁇ V 2 ) m -Q; wherein V 1 and V 2 are O, NR", -CO-, or -SO 2 -; Q is a 5-6 membered heterocyclic ring containing 1-2 heteroatoms selected from N or O; and J V1 and J V2 are halo.
  • J 2 is SO 2 CH3, morpholinyl, CH 2 OH, C(O)-(morpholinyl), C(O)NH(C 1-4 alkyl)OH, piperazinyl, CN, CH 2 NHC(O)CH 3 , halo, C(O)NH(C 1-4 alkyl)pyrrolidinyl, or SO 2 (pyrrolidinyl).
  • R 2 is phenyl or pyridyl.
  • J 2 is SO 2 (C 1-4 alkyl).
  • n is 0;
  • R 1 is benzimidazole, benzothiazole, benzoxazole, oxadiazole, isoxazole, thiadiazole, or triazole;
  • J 1 is halo, CN, NO 2 , or -V'-R;
  • R 2 is phenyl or pyridyl; m is 1 ; V 1 and V 2 are -SO 2 - -O-, -NR-, or -CO -;
  • Q is a 5-6 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen or oxygen;
  • R is H or C 1- 6alkyl wherein said C 1-6 alkyl is optionally substituted with 1-4 halo.
  • n is 1.
  • R 1 is phenyl, pyridyl, pyrimidyl, pyrazinyl, piperonyl, indolyl, benzimidazolyl, indazolyl, benzothiazolyl, benzothiophenyl (i.e. benzothienyl), benzoxazolyl, pyrrolopyrimidinyl, pyrrolopyridinyl, azaindazolyl, or azaindolyl.
  • R 1 is phenyl, pyridyl, pyrimidyl, piperonyl, or indole.
  • R 1 is phenyl.
  • R 1 is optionally substituted with 1-5 J 1 groups. In some embodiments, 1-3 J 1 groups, and in other embodiments, 1-2 J 1 groups.
  • J 1 is C 1-6 alkyl, CN, halo, OR, NR"R, SR, COR, CO 2 R, CONR"R, SOR, SO 2 R, S(O) 2 NR"R, OCOR, NRC(O)R, NRCOOR, NRSOR, NRSO 2 R, NRS0 2 NR"R, OCONR"R, or NRCONR”R; 4-6 membered fully saturated monocyclic ring containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur.
  • J 1 is C 1-6 alkyl, CN, halo, OR, NR"R, CONR"R, S(O) 2 NR 51 R, NC(O)R, or pyrrolidinyl.
  • R 2 is phenyl, pyridyl, pyrimidyl, indole, furanyl, pyrazole, thiophene, tetrahydropyran, or indazole.
  • R 2 is optionally substituted with 1-5 J 2 groups. In some embodiments, 1-3 J 2 groups, and in other embodiments, 1-2 J 2 groups.
  • J 2 is halo, CN, NR"R, C 1-6 alkyl, OR, SO 2 R, NHSO 2 R, COOR, CONR"R, morpholinyl, -V'-R, or -(V 2 ) m -Q, wherein
  • V 1 and V 2 are CO, -CONR"-, -CONR"-(C 1-4 alkyl)-,
  • R is H or C 1-4 alkyl
  • Q is 1,4-diazepanyl, azetidinyl optionally substituted with OMe, piperidinyl optionally substituted with C 1-4 alkyl, 4-CH 2 OH, CONH 2 , pyrrolidinyl, OH, or CH 2 -pyrrolidinyl; piperazinyl optionally substituted with CH 2 CH 2 CN, CH 3 , COCH 3 , pyrrolidinyl optionally substituted with dimethylamino, tetrahydropyran, C 3- iocyclolkyl optionally substituted with OH.
  • J 2 is SO 2 CH 3 , NHSO 2 CH 3 , CN, OH, OCH 3 , F, N(CH 3 ) 2 , NHSO 2 CH 3 , CF 3 , C 1-6 alkyl, CO(l ,4-diazepanyl), COOH, CONH 2 , CON(CH 3 ) 2) CO(azetidinyl), CON(CH 3 )(C 1-4 alky I)OCH 3 , CONH(C 1-4 alkyl)piperazinyl, CONH(C 1-4 alkyl)piperidinyl, CONH-tetrahydropyran, CON(methylpiperidinyl), CO(piperidinyl), CONH-cyclopropyl, CO(morpholinyl), CON(CH 3 )-(C 1-4 alkyl)-N(CH 3 ) 2 , CO(piperazinyl), CONH-(C 1-4 alkyl)-pyrrolidinyl, CONH
  • R 1 is phenyl
  • R 2 is phenyl, pyridyl, indole, furanyl, pyrazole, thiophene, tetrahydropyran, or indazole
  • J 2 is halo, CN, NR"R, C 1-6 alkyl, OR, SO 2 R, NHSO 2 R, COOR, CONR"R, morpholinyl, -V'-R, or -(V 2 ) m -Q, wherein m is 1
  • V 1 and V 2 are CO, -CONR-, -CONR-(C 1-4 alkyl)-, -CONR-(C 1-4 alky I)-OCH 2 -, or -CONR-(C 1-4 alky I)-N(CH 3 )-
  • R is H or C 1-4 alkyl
  • R is H or C 1-4 alkyl
  • Q is 1 ,4-diazepanyl, azetidinyl optionally substituted with OMe, piperidinyl optionally substituted with C 1-4 alkyl, 4-CH 2 OH, CONH 2 , pyrrolidinyl, OH, or CH 2 -pyrrolidinyl; piperazinyl optionally substituted with CH 2 CH 2 CN, CH 3 , COCH 3 , pyrrolidinyl optionally substituted with dimethylamino, tetrahydropyran, C 3-10 cyclolkyl optionally substituted with OH; and J 1 is C 1-6 alkyl, CN, halo, OR, NR"R, SR, COR, CO 2 R, CO NR"R, SOR,
  • n is O;
  • R 1 is benzimidazole, benzothioazole, oxadiazole, isoxazole, or triazole;
  • J 1 is halo, CN, NO 2 , or -V'-R;
  • R 2 is phenyl or pyridyl
  • J 2 is — (V 2 ) m -Q or -V'-R; m is 1 ;
  • V 1 and V 2 are -SO 2 -, -O-, -NR"-, or -CO -;
  • Q is a 5-6 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen or oxygen;
  • R is H or C 1-6 alkyl wherein said C 1- 6alkyl is optionally substituted with 1-4 halo.
  • the compound is selected from Table 1 (above).
  • One aspect of this invention provides compounds that are inhibitors of ATR kinase, and thus are useful for treating or lessening the severity of a disease, condition, or disorder where ATR is implicated in the disease, condition, or disorder.
  • Another aspect of this invention provides compounds that are useful for the treatment of diseases, disorders, and conditions characterized by excessive or abnormal cell proliferation. Such diseases include, a proliferative or hyperproliferative disease. Examples of proliferative and hyperproliferative diseases include, without limitation, cancer and myeloproliferative disorders.
  • said compounds are selected from the group consisting of a compound of formula I, II, III, IV, IA, HA, IHA, IVA, IA-i, IA-ii, IA-iii, V, VI, and VII.
  • cancer includes, but is not limited to the following cancers.
  • Oral buccal cavity, lip, tongue, mouth, pharynx; Cardiac: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma; Lung: bronchogenic carcinoma (squamous cell or epidermoid, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma; Gastrointestinal: esophagus (squamous cell carcinoma, larynx, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leiomyosarcoma), pan
  • the term "cancerous cell” as provided herein includes a cell afflicted by any one of the above-identified conditions.
  • the cancer is selected from colorectal, thyroid, or breast cancer.
  • myeloproliferative disorders includes disorders such as polycythemia vera, thrombocythemia, myeloid metaplasia with myelofibrosis, hypereosinophilic syndrome, juvenile myelomonocytic leukemia, systemic mast cell disease, and hematopoietic disorders, in particular, acute-myelogenous leukemia (AML), chronic-myelogenous leukemia (CML), acute-promyelocytic leukemia (APL), and acute lymphocytic leukemia (ALL).
  • AML acute-myelogenous leukemia
  • CML chronic-myelogenous leukemia
  • APL acute-promyelocytic leukemia
  • ALL acute lymphocytic leukemia
  • compositions to treat or prevent the herein identified disorders.
  • the compounds of this invention can also exist as pharmaceutically acceptable derivatives.
  • a "pharmaceutically acceptable derivative” is an adduct or derivative which, upon administration to a patient in need, is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite or residue thereof.
  • pharmaceutically acceptable derivatives include, but are not limited to, esters and salts of such esters.
  • a "pharmaceutically acceptable derivative or prodrug” means any pharmaceutically acceptable ester, salt of an ester or other derivative or salt thereof of a compound, of this invention which, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof.
  • Particularly favoured derivatives or prodrugs are those that increase the bioavailability of the compounds of this invention when such compounds are administered to a patient (e.g., by allowing an orally administered compound to be more readily absorbed into the blood) or which enhance delivery of the parent compound to a biological compartment (e.g., the brain or lymphatic system) relative to the parent species.
  • Pharmaceutically acceptable prodrugs of the compounds of this invention include, without limitation, esters, amino acid esters, phosphate esters, metal salts and sulfonate esters.
  • the present invention also provides compounds and compositions that are useful as inhibitors of ATR kinase.
  • compositions that comprise any of the compounds as described herein, and optionally comprise a pharmaceutically acceptable carrier, adjuvant or vehicle.
  • the pharmaceutically acceptable carrier, adjuvant, or vehicle includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired.
  • Some examples of materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, or potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, polyacrylates, waxes, polyethylene- polyoxypropylene-block polymers, wool fat, sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin;
  • Another aspect of this invention is directed towards a method of treating cancer in a subject in need thereof, comprising administration of a compound of this invention or a pharmaceutically acceptable salt thereof, and an additional therapeutic agent.
  • said method comprises the sequential or co-administration of the compound or a pharmaceutically acceptable salt thereof, and the additional therapeutic agent.
  • said additional therapeutic agent is an anti-cancer agent.
  • said additional therapeutic agent is a DNA-damaging agent.
  • said additional therapeutic agent is selected from radiation therapy, chemotherapy, or other agents typically used in combination with radiation therapy or chemotherapy, such as radiosensitizers and chemosensitizers.
  • radiosensitizers are agents that can be used in combination with radiation therapy. Radiosensitizers work in various different ways, including, but not limited to, making cancer cells more sensitive to radiation therapy, working in synergy with radiation therapy to provide an improved synergistic effect, acting additively with radiation therapy, or protecting surrounding healthy cells from damage caused by radiation therapy. Likewise chemosensitizers are agents that can be used in combination with chemotherapy. Similarly, chemosensitizers work in various different ways, including, but not limited to, making cancer cells more sensitive to chemotherapy, working in synergy with chemotherapy to provide an improved synergistic effect, acting additively to chemotherapy, or protecting surrounding healthy cells from damage caused by chemotherapy.
  • DNA-damaging agents examples include, but are not limited to Platinating agents, such as Carboplatin, Nedaplatin, Satraplatin and other derivatives; Topo I inhibitors, such as Topotecan, irinotecan/SN38, rubitecan and other derivatives; Antimetabolites, such as Folic family (Methotrexate, Pemetrexed and relatives); Purine antagonists and Pyrimidine antagonists (Thioguanine, Fludarabine, Cladribine, Cytarabine, Gemcitabine, 6-Mercaptopurine, 5-Fluorouracil (5FU) and relatives); Alkylating agents, such as Nitrogen mustards (Cyclophosphamide, Melphalan, Chlorambucil, mechlorethamine, Ifosfamide and relatives); nitrosoureas (eg Carmustine); Triazenes (Dacarbazine, temozolomide); Alkyl
  • therapies or anticancer agents that may be used in combination with the inventive agents of the present invention include surgery, radiotherapy (in but a few examples, gamma-radiation, neutron beam radiotherapy, electron beam radiotherapy, proton therapy, brachy therapy, and systemic radioactive isotopes, to name a few), endocrine therapy, biologic response modifiers (interferons, interleukins, and tumor necrosis factor (TNF) to name a few), hyperthermia and cryotherapy, agents to attenuate any adverse effects (e.g., antiemetics), and other approved chemotherapeutic drugs, including, but not limited to, the DNA damaging agents listed herein, spindle poisons (Vinblastine, Vincristine, Vinorelbine, Paclitaxel), podophyllotoxins (Etoposide, Irinotecan, Topotecan), nitrosoureas (Carmustine, Lomustine), inorganic ions (Cisplatin, Carboplatin, Carb
  • a compound of the instant invention may also be useful for treating cancer in combination with any of the following therapeutic agents: abarelix (Plenaxis depot®); aldesleukin (Prokine®); Aldesleukin (Proleukin®); Alemtuzumabb (Campath®); alitretinoin (Panretin®); allopurinol (Zyloprim®); altretamine (Hexalen®); amifostine (Ethyol®); anastrozole (Arimidex®); arsenic trioxide (Trisenox®); asparaginase (Elspar®); azacitidine (Vidaza®); bevacuzimab (Avastin®); bevacuzimab (Avastin®); bexarotene capsules (Targretin®); bexarotene gel (Targretin®); bleomycin (Blenoxane®); bortezomib (Velcade®); busulfan intra
  • the ATR kinase inhibitors or pharmaceutical salts thereof may be formulated into pharmaceutical compositions for administration to animals or humans. These pharmaceutical compositions, which comprise an amount of the ATR inhibitor effective to treat or prevent the diseases or conditions described herein and a pharmaceutically acceptable carrier, are another embodiment of the present invention.
  • the exact amount of compound required for treatment will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the infection, the particular agent, its mode of administration, and the like.
  • the compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage.
  • dosage unit form refers to a physically discrete unit of agent appropriate for the patient to be treated.
  • the specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts.
  • patient means an animal, preferably a mammal, and most preferably a human.
  • these compositions optionally further comprise one or more additional therapeutic agents.
  • additional therapeutic agents chemotherapeutic agents or other antiproliferative agents may be combined with the compounds of this invention to treat proliferative diseases and cancer. Examples of known agents with which these compositions can be combined are listed above under the "Combination Therapies" section and also throughout the specification. Some embodiments provide a simultaneous, separate or sequential use of a combined preparation. Modes of Administration and Dosage Forms
  • compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated.
  • the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
  • Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs.
  • the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
  • the oral compositions can also include
  • Injectable preparations for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents.
  • the sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol.
  • acceptable vehicles and solvents that may be employed are water, Ringer's solution, U. S. P. and isotonic sodium chloride solution.
  • sterile, fixed oils are conventionally employed as a solvent or suspending medium.
  • injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
  • a compound of the present invention In order to prolong the effect of a compound of the present invention, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution that, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenteral Iy administered compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the compound in biodegradable polymers such as polylactide-polyglycolide.
  • the rate of compound release can be controlled.
  • biodegradable polymers include poly(orthoesters) and poly(anhydrides).
  • Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues.
  • compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
  • suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
  • Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
  • the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar—agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol
  • the dosage form may also comprise buffering agents.
  • Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
  • the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner.
  • embedding compositions examples include polymeric substances and waxes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
  • the active compounds can also be in microencapsulated form with one or more excipients as noted above.
  • the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art.
  • the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch.
  • Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose.
  • the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner.
  • buffering agents include polymeric substances and waxes.
  • Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches.
  • the active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required.
  • Ophthalmic formulation, eardrops, and eye drops are also contemplated as being within the scope of this invention.
  • the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body.
  • Such dosage forms can be made by dissolving or dispensing the compound in the proper medium.
  • compositions of the present invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir.
  • parenteral as used herein includes, but is not limited to, subcutaneous, intravenous, intramuscular, intra-articular, intra-synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques.
  • the compositions are administered orally, intraperitoneally or intravenously.
  • Sterile injectable forms of the compositions of this invention may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents.
  • the sterile injectable preparation may also be a sterile injectable solution or suspension in a nontoxic parenteral ly-acceptable diluent or solvent, for example as a solution in 1 ,3-butanediol.
  • the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution.
  • sterile, fixed oils are conventionally employed as a solvent or suspending medium.
  • any bland fixed oil may be employed including synthetic mono- or di-glycerides.
  • Fatty acids such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutical ly-acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions.
  • These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents which are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions.
  • Other commonly used surfactants such as Tweens, Spans and other emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms may also be used for the purposes of formulation.
  • compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, aqueous suspensions or solutions.
  • carriers commonly used include, but are not limited to, lactose and corn starch.
  • Lubricating agents such as magnesium stearate, are also typically added.
  • useful diluents include lactose and dried cornstarch.
  • aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents may also be added.
  • the pharmaceutical compositions of this invention may be administered in the form of suppositories for rectal administration. These can be prepared by mixing the agent with a suitable non-irritating excipient that is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug. Such materials include, but are not limited to, cocoa butter, beeswax and polyethylene glycols.
  • a suitable non-irritating excipient that is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug.
  • Such materials include, but are not limited to, cocoa butter, beeswax and polyethylene glycols.
  • the pharmaceutical compositions of this invention may also be administered topically, especially when the target of treatment includes areas or organs readily accessible by topical application, including diseases of the eye, the skin, or the lower intestinal tract. Suitable topical formulations are readily prepared for each of these areas or organs.
  • Topical application for the lower intestinal tract can be effected in a rectal
  • the pharmaceutical compositions may be formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers.
  • Carriers for topical administration of the compounds of this invention include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water.
  • the pharmaceutical compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers.
  • Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.
  • the pharmaceutical compositions may be formulated as micronized suspensions in isotonic, pH adjusted sterile saline, or, preferably, as solutions in isotonic, pH adjusted sterile saline, either with or without a preservative such as benzylalkonium chloride.
  • the pharmaceutical compositions may be formulated in an ointment such as petrolatum.
  • the pharmaceutical compositions of this invention may also be administered by nasal aerosol or inhalation.
  • compositions are prepared according to techniques well- known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents.
  • the amount of protein kinase inhibitor that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated, the particular mode of administration.
  • the compositions should be formulated so that a dosage of between 0.01 - 100 mg/kg body weight/day of the inhibitor can be administered to a patient receiving these compositions.
  • a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated.
  • the amount of inhibitor will also depend upon the particular compound in the composition.
  • additional drugs which are normally administered to treat or prevent that condition, may be administered together with the compounds of this invention.
  • those additional agents may be administered separately, as part of a multiple dosage regimen, from the protein kinase inhibitor-containing compound or composition.
  • those agents may be part of a single dosage form, mixed together with the protein kinase inhibitor in a single composition.
  • Another aspect of this invention is directed towards a method of treating cancer in a subject in need thereof, comprising the sequential or co-administration of a compound of this invention or a pharmaceutically acceptable salt thereof, and an anti-cancer agent.
  • said anti-cancer agent is selected from Platinating agents, such as Cisplatin, Oxaliplatin, Carboplatin, Nedaplatin, or Satraplatin and other derivatives; Topo I inhibitors, such as Camptothecin, Topotecan, irinotecan/SN38, rubitecan and other derivatives; Antimetabolites, such as Folic family (Methotrexate, Pemetrexed and relatives); Purine family (Thioguanine, Fludarabine, Cladribine, 6-Mercaptopurine and relatives); Pyrimidine family (Cytarabine, Gemcitabine, 5-Fluorouracil and relatives); Alkylating agents, such as Nitrogen mustards (Cyclophosphamide, Melphalan,
  • Carmustine Triazenes (Dacarbazine, temozolomide); Alkyl sulphonates (e.g. Busulfan); Procarbazine and Aziridines; Antibiotics, such as Hydroxyurea; Anthracyclines (doxorubicin, daunorubicin, epirubicin and other derivatives); Anthracenediones (Mit ⁇ xantrone and relatives); Streptomyces family (Bleomycin, Mitomycin C, actinomycin) and Ultraviolet light.
  • Triazenes Dacarbazine, temozolomide
  • Alkyl sulphonates e.g. Busulfan
  • Procarbazine and Aziridines Antibiotics, such as Hydroxyurea
  • Anthracyclines doxorubicin, daunorubicin, epirubicin and other derivatives
  • Anthracenediones Mit ⁇ xantrone and relatives
  • Streptomyces family Boleomycin
  • the compounds and compositions of this invention are also useful in biological samples.
  • One aspect of the invention relates to inhibiting ATR kinase activity in a biological sample, which method comprises contacting said biological sample with a compound described herein or a composition comprising said compound.
  • biological sample means an in vitro or an ex vivo sample, including, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
  • compounds described herein includes compounds of formula I, II, III, IV, IA, HA, IHA, IVA, IA- ⁇ , IA-ii, IA-iii, V, VI, and VII.
  • Inhibition of ATR kinase activity in a biological sample is useful for a variety of purposes that are known to one of skill in the art. Examples of such purposes include, but are not limited to, blood transfusion, organ-transplantation, and biological specimen storage. Study of Protein Kinases
  • Another aspect of this invention relates to the study of protein kinases in biological and pathological phenomena; the study of intracellular signal transduction pathways mediated by such protein kinases; and the comparative evaluation of new protein kinase inhibitors.
  • uses include, but are not limited to, biological assays such as enzyme assays and cell-based assays.
  • the activity of the compounds as protein kinase inhibitors may be assayed in vitro, in vivo or in a cell line.
  • In vitro assays include assays that determine inhibition of either the kinase activity or ATPase activity of the activated kinase. Alternate in vitro assays quantitate the ability of the inhibitor to bind to the protein kinase and may be measured either by radiolabelling the inhibitor prior to binding, isolating the inhibitor/kinase complex and determining the amount of radiolabel bound, or by running a competition experiment where new inhibitors are incubated with the kinase bound to known radioligands.
  • Detailed conditions for assaying a compound utilized in this invention as an inhibitor of ATR is set forth in the Examples below.
  • Another aspect of the invention provides a method for modulating enzyme activity by contacting a compound described herein with ATR kinase.
  • the present invention provides a method for treating or lessening the severity of a disease, condition, or disorder where ATR kinase is implicated in the disease state. In another aspect, the present invention provides a method for treating or lessening the severity of an ATR kinase disease, condition, or disorder where inhibition of enzymatic activity is implicated in the treatment of the disease. In another aspect, this invention provides a method for treating or lessening the severity of a disease, condition, or disorder with compounds that inhibit enzymatic activity by binding to the ATR kinase.
  • Another aspect provides a method for treating or lessening the severity of a kinase disease, condition, or disorder by inhibiting enzymatic activity of ATR kinase with an ATR kinase inhibitor.
  • One aspect of the invention relates to a method of inhibiting ATR kinase activity in a patient, which method comprises administering to the patient a compound described herein, or a composition comprising said compound. In some embodiments, said method is used to treat or prevent a condition selected from proliferative and hyperproliferative diseases, such as cancer.
  • Another aspect of this invention provides a method for treating, preventing, or lessening the severity of proliferative or hyperproliferative diseases comprising administering an effective amount of a compound, or a pharmaceutically acceptable composition comprising a compound, to a subject in need thereof.
  • said subject is a patient.
  • patient means an animal, preferably a human.
  • said method is used to treat or prevent cancer.
  • said method is used to treat or prevent a type of cancer with solid tumors.
  • said cancer is selected from the following cancers: Oral: buccal cavity, lip, tongue, mouth, pharynx; Cardiac: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma; Lung: bronchogenic carcinoma (squamous cell or epidermoid, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma; Gastrointestinal: esophagus (squamous cell carcinoma, larynx, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma,
  • the cancer is selected from the cancers described herein.
  • said cancer is lung cancer, head and neck cancer, pancreatic cancer, gastric cancer, or brain cancer.
  • an "effective amount" of the compound or pharmaceutically acceptable composition is that amount effective in order to treat said disease.
  • the compounds and compositions, according to the method of the present invention may be administered using any amount and any route of administration effective for treating or lessening the severity of said disease.
  • One aspect provides a method for inhibiting ATR in a patient comprising administering a compound described herein as described herein.
  • Another embodiment provides a method of treating cancer comprising administering to a patient a compound described herein, wherein the variables are as defined herein.
  • Some embodiments comprising administering to said patient an additional therapeutic agent selected from a DNA-damaging agent; wherein said additional therapeutic agent is appropriate for the disease being treated; and said additional therapeutic agent is administered together with said compound as a single dosage form or separately from said compound as part of a multiple dosage form.
  • said DNA-damaging agent is selected from ionizing radiation, radiomimetic neocarzinostatin, a platinating agent, a Topo I inhibitor, a Topo II inhibitor, an antimetabolite, an alkylating agent, an alkyl sulphonates, an antimetabolite, or an antibiotic.
  • said DNA-damaging agent is selected from ionizing radiation, a platinating agent, a Topo I inhibitor, a Topo II inhibitor, or an antibiotic.
  • Platinating agents include Cisplatin, Oxaliplatin, Carboplatin,
  • Other platinatirfg agents include Lobaplatin, and Triplatin.
  • Other platinating agents include Tetranitrate, Picoplatin, Satraplatin,
  • Topo I inhibitor examples include Camptothecin, Topotecan, irinotecan/SN38, rubitecan and other derivatives.
  • Other Topo I inhibitors include Belotecan.
  • Topo II inhibitors examples include Etoposide, Daunorubicin, Doxorubicin,
  • Antimetabolites include members of the Folic family, Purine family
  • Examples of the Folic family include methotrexate, pemetrexed and relatives; examples of the Purine family include Thioguanine, Fludarabine, Cladribine, 6-Mercaptopurine, and relatives; examples of the Pyrimidine family include Cytarabine, gemcitabine, 5-Fluorouracil (5FU) and relatives.
  • antimetabolites include Aminopterin,
  • alkylating agents include Nitrogen mustards, Triazenes, alkyl sulphonates, Procarbazine and Aziridines.
  • Nitrogen mustards include Nitrogen mustards, Triazenes, alkyl sulphonates, Procarbazine and Aziridines.
  • Nitrogen mustards include Nitrogen mustards, Triazenes, alkyl sulphonates, Procarbazine and Aziridines.
  • alkylating agents include Mechlorethamine, Cyclophosphamide, Ifosfamide, Trofosfamide, Chlorambucil, Melphalan, Prednimustine, Bendamustine, Uramustine, Estramustine, Carmustine, Lomustine, Semustine, Fotemustine, Nimustine, Ranimustine, Streptozocin, Busulfan, Mannosulfan, Treosulfan, Carboquone, ThioTEPA, Triaziquone, Triethylenemelamine, Procarbazine, dacarbazine, Temozolomide, Altretamine, Mitobronitol, Actinomycin, Bleomycin, Mitomycin and Plicamycin.
  • antibiotics include Mitomycin, Hydroxyurea; Anthracyclines, Anthracenediones, Streptomyces family.
  • Anthracyclines include doxorubicin, daunorubicin, epirubicin and other derivatives; examples of Anthracenediones include Mitoxantrone and relatives; examples of Streptomyces family includee Bleomycin, Mitomycin C, and actinomycin.
  • said platinating agent is Cisplatin or Oxaliplatin; said Topo I inhibitor is Camptothecin; said Topo Il inhibitor is Etoposide; and said antibiotic is Mitomycin.
  • said platinating agent is selected from Cisplatin, Oxaliplatin, Carboplatin, Nedaplatin, or Satraplatin; said Topo I inhibitor is selected from Camptothecin, Topotecan, irinotecan/SN38, rubitecan; said Topo II inhibitor is selected from Etoposide; said antimetabolite is selected from a member of the Folic Family, the Purine Family, or the Pyrimidine Family; said alkylating agent is selected from nitrogen mustards, nitrosoureas, triazenes, alkyl sulfonates, Procarbazine, or aziridines; and said antibiotic is selected from Hydroxyurea, Anthracyclines, Anthracenediones, or Streptomyces family.
  • the method is used on a cancer cell having defects in the ATM signaling cascade.
  • said defect is altered expression or activity of one or more of the following: ATM, p53, CHK2, MREl 1 , RAD50, NBSl, 53BP1, MDCl or H2AX.
  • the cell is a cancer cell expressing DNA damaging oncogenes.
  • said cancer cell has altered expression or activity of one or more of the following: K-Ras, N-Ras, H-Ras, Raf, Myc, Mos, E2F, Cdc25A, CDC4, CDK2, Cyclin E, Cyclin A and Rb.
  • Yet another embodiment provides use of a compound described herein as a radio- sensitizer or a chemo-sensitizer.
  • a compound of formula I as a single agent (monotherapy) for treating cancer.
  • the compounds of formula I are used for treating patients having cancer with a DNA-damage response (DDR) defect.
  • said defect is a mutation or loss of ATM, p53, CHK2, MREl 1, RAD50, NBSl, 53BP1, MDCl, or H2AX.
  • the compounds of the disclosure may be prepared in light of the specification using steps generally known to those of ordinary skill in the art. Those compounds may be analyzed by known methods, including but not limited to LCMS (liquid chromatography mass spectrometry) and NMR (nuclear magnetic resonance). Below are a set of generic schemes that illustrate generally how to prepare the compounds of the present disclosure. Scheme I-Al : Preparation of Compounds wherein -L-R 1 is an Aromatic Amide
  • Cyclic amides compounds of the present disclosure wherein -L-R 1 is an aromatic amide can be prepared according to methods similar to the one depicted in Scheme I-Al : Commercially available ester 1 is reacted with a boronic acid under Suzuki conditions to give intermediate 2. The carboxylic acid group is engaged in a coupling reaction with an amine to lead to cyclic amide compounds of the Formula I.
  • R is -(L-NR 1 ROp or -(J 2 ) q
  • R is -(L-NR 1 ROp or -(J 2 ),
  • rings A is a 1,3,4- oxadiazole
  • Scheme I-B2 a variation of the synthetic sequence depicted in scheme I-Bl .
  • the acylhydrazide then undergoes a cyclodehydration to lead to compounds of formula I.
  • R is -(L-NR 1 R 2 ) p or ⁇ h ⁇
  • R is -(L-NR 1 ROp or - ⁇ J 2 ) q
  • Ring A is a 1 ,2,4-oxadiazole
  • Scheme I-Cl nitrile 2 reacts with hydroxylamine to give intermediate 13.
  • the hydroxy group in 13 reacts with acid chlorides to lead to intermediate 14 which undergoes cyclodehydration to afford compounds of formula I.
  • rings A is a 1 ,2,4- oxadiazole
  • Scheme I-C2 Commercially available nitrile 1 reacts with hydroxylamine to give intermediate 1J5.
  • the hydroxy group in 15 reacts with acid chlorides to lead to intermediate .16 which undergoes cyclodehydration to afford intermediate 12-
  • the bromo handle in 17 is then used to perform a Suzuki reaction with a boronic acid coupling partner to give compounds of formula I.
  • R group in I contains a carboxylic acid moiety, it can be further transformed (eg into an amide) using conditions known in the art.
  • R is -(L-NR 1 R ⁇ p or - ⁇ J 2 ) q
  • R is -(L-NR l ' ⁇ R> 2 ⁇ N ) p or -(J 2 ),
  • rings A is 1,3,4- thiadiazole
  • the acid functional group in 10 is engaged into coupling with a suitable partner (R5CSNHNH 2 ) to form the thioacylhydrazide intermediate , 19.
  • R5CSNHNH 2 a suitable partner
  • cyclodehydration leads to the compound 20 where the 1,3,4-thiadiazole ring has been constructed. Transformation of starting point 10 into 20 has been performed in a one-pot procedure using reagents serving two purposes (coupling and cyclodehydration).
  • the bromo handle in thiadiazole 20 is then reacted with a boronic acid under Suzuki conditions to give compounds of formula I.
  • R group in I contains a carboxylic acid moiety, it can be further transformed (eg into an amide) using conditions known in the art.
  • R is -(L-NR'R 2 ) P or -(J 2 ) q
  • compounds of the present disclosure where Ring A is an isoxazole can be prepared according to methods similar to the one depicted in Scheme I-E2:
  • the TMS- protected intermediate 23, described in scheme I-El can be deprotected to reveal the alkyne compound 25.
  • the alkyne 25 reacts in a cyclocondensation with N-hydroxyaroyl chloride to furnish intermediate 26 where the isoxazole ring has been constructed.
  • the bromo handle in isoxazole 26 is then reacted with a boronic acid under Suzuki conditions to give compounds 27.
  • a final deprotection of N-protecting groups in 27 can reveal compounds of Formula I.
  • R group in I contains a carboxylic acid moiety, it can be further transformed (eg into an amide) using conditions known in the art.
  • R is -(L-NR'R 2 ) P Or -(J 2 ⁇
  • Ring A is a 1,2,4- triazole
  • Ester 3 is reacted with a boronic acid under Suzuki conditions to give intermediate 4.
  • R group contains a carboxylic acid moiety, it can be further . transformed at this stage (eg into an amide) using conditions known in the art.
  • the methyl ester group in 4 is then transformed into an hydrazide by reaction with hydrazine to give 5.
  • the hydrazide group in 5 is engaged in a coupling reaction with a nitrile and subsequently undergoes a cyclodehydration to lead to compounds of formula I.
  • R is -(L-NR 1 R 2 ) p or -(J 2 ) q
  • rings A is a 1 ,2,4- triazole
  • R functional group in I- or 3 nitrile and methyl ester respectively
  • R5CONHNH2 when starting from 1; R5CN if using 6
  • R5CONHNH2 when starting from 1; R5CN if using 6
  • R5CONHNH2 when starting from 1; R5CN if using 6
  • R5CONHNH2 when starting from 1; R5CN if using 6
  • the bromo handle in triazole 7 is then reacted with a boronic acid under Suzuki conditions to give compounds of formula I.
  • R group in I contains a carboxylic acid moiety, it can be further transformed (eg into an amide) using conditions known in the art.
  • R is -(L-NR 1 R 2 Jp or -(J 2 ) q
  • Benzoxazole compounds of Formula VI can be prepared according to methods similar to the one depicted in Scheme I-Gl : Commercially available nitrile 1 is reacted with a amino phenol to give the benzoxazole which is then reacted with a boronic acid under Suzuki conditions to give compounds of the formula VI.
  • Benzothiazole compounds of Formula VI can be prepared according to methods similar to the one depicted in Scheme I-Hl : Commercially available nitrile J, is reacted with a aminobenzenethiol to give the benzothiazole which is then reacted with a boronic acid under Suzuki conditions to give compounds of the formula VI.
  • Scheme I-H2 preparation of compounds where benzothiazole
  • benzothiazole compounds of Formula Vl can be prepared according to Scheme I-H2; methyl ester 3 is reacted with a boronic acid under Suzuki conditions to give intermediate 8. Cyclisation of intermediate 8 with an amino benzenethiol will lead to compounds of the formula VI.
  • R is -(L-NR 1 R 2 ),, or -CJ 2 ) q
  • Benzimidazole compounds of Formula I can be prepared according to methods similar to the one depicted in Scheme I-Il : methyl ester 3 is reacted with a boronic acid under Suzuki conditions to give intermediate 8. Cyclisation of internediate 8 with a benzene 1 ,2-diamine will lead to compounds of the formula I
  • R is -(L-NR'R 2 ) P or -(J 2 ) q
  • benzimidazole compounds of Formula I can be prepared according to methods similar to the one depicted in Scheme 1-12: Reaction of the acid functional group of 3 is reacted with a benzene 1,2-diamine to give the benzimidazole intermediate 9. Intermediate 9 is then reacted with a boronic acid under Suzuki conditions to give compounds of the formula I.
  • Rt(min) refers to the HPLC retention time, in minutes, associated with the compound. Unless otherwise indicated, the HPLC method utilized to obtain the reported retention time is as follows:
  • Mass spec, samples were analyzed on a MicroMass Quattro Micro mass spectrometer operated in single MS mode with electrospray ionization. Samples were introduced into the mass spectrometer using chromatography. Mobile phase for all mass . spec, analyses consisted of 1OmM pH 7 ammonium acetate and a 1 :1 acetonitrile-methanol mixture, column gradient conditions are 5%-100% acetonitrile-methanol over 3.5 mins gradient time and 5 mins run time on an ACE C8 3.0 x 75mm column. Flow rate is 1.2 ml/min.
  • Step 1 Methyl 3-amino-6-bromopyrazine-2-carboxylate
  • Step 2 S-amino- ⁇ -bromopyrazine- ⁇ -carboxylic acid
  • Step 3 S-amino- ⁇ -bromo-N-phenylpyrazine-1-carboxamide
  • a Greenhouse tube was charged with 4-Methoxyphenylboronic acid (31.4 mg, 0.207 mmol) and treated with a solution of dichloropalladium;triphenylphosphane (4.84 mg, 0.0069 mmol) and 3-amino-6-bromo-N-phenyl-pyrazine-2-carboxamide (40.45 mg, 0.138 mmol) in DMF (0.81 mL) followed by Na 2 CO 3 (2M solution, 207uL, 0.414 mmol). The mixture was flushed with nitrogen and heated to 88 °C for 18 hours.
  • Compound 1-42 was prepared by using Method A, Steps 1-3 followed by Method I-B, Steps 1-2.
  • Step 1 3-(5-amino-6-(phenylcarbamoyl)pyrazin-2-yl)benzoic acid
  • Step 2 (R)-3-amino-N-pheny l-6-(4-(2-(pyrrolidin- 1 -ylmethy l)pyrrolidine- 1 - carbonyl)phenyl)pyrazine-2-carboxamide
  • Step 1 Methyl 3-amino-6-(4-(methylsulfonyl)phenyl)pyrazine-2-carboxylate
  • Compound 1-109 was prepared using Method A, Step 1 followed by Method I-C, Steps 1-2 followed by Method I-D, Step 1.
  • Compound 122 was prepared using Method A, Steps 1-2 followed by Method I-E, Steps 1-2.
  • Step 1 3-(l H-benzo[d]imidazol-2-yl)-5-bromopyrazin-2-amine
  • Compound 138 was prepared using Method A, Step 1 followed by Method I-F, Steps 1 -4.
  • Step 1 3-amino-6-(pyridin-3-yl)pyrazine-2-carboxylic acid
  • Step 4 3-(5-phenyl-4H-1,2,4-triazol-3-yl)-5-(pyridin-3-yl)pyrazin-2-amine (Compound 138)
  • Step 4 5-(4-(methylsulfonyl)phenyl)-3-(5-phenyl-l ,2,4-oxadiazol-3-yl)pyrazin-2-amine (Compound 144)
  • PdCl 2 (PPh 3 )2 (1 1.03 mg, 0.01572 mmol) in DMF (2 mL) was treated with Na 2 CO 3 (471.4 ⁇ L of 2 M, 0.9429 mmol) and the reaction placed under an atmosphere of nitrogen and heated at
  • Compound IIA-1 was also prepared using Method I-G.
  • Compound 1-146 was prepared using Method A, Steps 1 followed by Method I-C, Steps 1-2 followed by Method I-H, Step 1.
  • Step 1 4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)benzoic acid
  • Step 1 (4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)phenyl) (1,4- diazepan-1-yl)methanone
  • tert-butyl 4-[4-[5-amino-6-(6-methyl- 1 H-benzimidazol-2-yl)pyrazin-2- yl]benzoyl]-1,4-diazepane-1-carboxylate (117 mg, 0.2218 mmol) was dissolved in DCM (3 mL) and the mixture was cooled to 0°C. TFA (3 mL, 38.94 mmol) was added and the reaction mixture was allowed to warm to room temperature and stirred for a further 2 hours.
  • Example 1 1 3-amino-6-(4-(4-(dimethylamino)piperidine-1-carbonyl)pheny I)-N- phenylpyrazine-2-carboxamide (Compound 11-10)
  • Step 3 S-amino- ⁇ -bromo-N-phenylpyrazine-1-carboxamide
  • Step 4 4-(5-amino-6-(phenylcarbamoyl)pyrazin-2-yl)benzoic acid
  • Step 5 3-amino-6-(4-(4-(dimethyIamino)piperidine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide
  • N,N-dimethylpiperidin-4-amine (57.54mg, 0.449 mmol) was weighed into a greenhouse tube and treated with a solution of 4-(5-amino-6-(phenylcarbamoyl)pyrazin-2- yl)benzoic acid (50mg, 0.150 mmol), CDI (48.5 lmg, 0.299 mmol) and DMAP (1.82mg, 0.015 mmol) in DMSO (ImL of a stock solution). DIPEA (78.2uL, 0.449 mmol) was then added and the mixture stirred at 38°C for 6 hours.

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Abstract

The present invention relates to pyrazine compounds useful as inhibitors of ATR protein kinase. The invention also relates to pharmaceutically acceptable compositions comprising the compounds of this invention; methods of treating of various diseases, disorders, and conditions using the compounds of this invention; processes for preparing the compounds of this invention; intermediates for the preparation of the compounds of this invention; and methods of using the compounds in in vitro applications, such as the study of kinases in biological and pathological phenomena; the study of intracellular signal transduction pathways mediated by such kinases; and the comparative evaluation of new kinase inhibitors. The compounds of this invention have formula (I):wherein the variables are as defined herein.

Description

PYRAZINE DERIVATIVES USEFUL AS INHIBITORS OF ATR KINASE
BACKGROUND OF THE INVENTION
[0001] ATR ("ATM and Rad3 related") kinase is a protein kinase involved in cellular responses to DNA damage. ATR kinase acts with ATM ("ataxia telangiectasia mutated") kinase and many other proteins to regulate a cell's response to DNA damage, commonly referred to as the DNA Damage Response ("DDR"). The DDR stimulates DNA repair, promotes survival and stalls cell cycle progression by activating cell cycle checkpoints, which provide time for repair. Without the DDR, cells are much more sensitive to DNA damage and readily die from DNA lesions induced by endogenous cellular processes such as DNA replication or exogenous DNA damaging agents commonly used in cancer therapy. [0002] Healthy cells can rely on a host of different proteins for DNA repair including the DDR kinase ATR. In some cases these proteins can compensate for one another by activating functionally redundant DNA repair processes. On the contrary, many cancer cells harbour defects in some of their DNA repair processes, such as ATM signaling, and therefore display a greater reliance on their remaining intact DNA repair proteins which include ATR. [0003] In addition, many cancer cells express activated oncogenes or lack key tumour suppressors, and this can make these cancer cells prone to dysregulated phases of DNA replication which in turn cause DNA damage. ATR has been implicated as a critical component of the DDR in response to disrupted DNA replication. As a result, these cancer cells are more dependent on ATR activity for survival than healthy cells. Accordingly, ATR inhibitors may be useful for cancer treatment, either used alone or in combination with DNA damaging agents, because they shut down a DNA repair mechanism that is more important for cellular survival in many cancer cells than in healthy normal cells. In fact, ATR inhibition has been shown to be effective in cancer cells as single agents and as potent sensitizers to radiotherapy and genotoxic chemotherapy.
[0004] ATR peptide can be expressed and isolated using a variety of methods known in the literature (see e.g., Unsal-Kacmaz et al, PNAS 99: 10, pp6673-6678, May 14, 2002; see also Kumagai et al. Cell 124, pp943-955, March 10, 2006; Unsal-Kacmaz et al. Molecular and Cellular Biology. Feb 2004, pl292-1300; and Hall-Jackson et al. Oncogene 1999, 18,
6707-6713).
[0005] For all of these reasons, there is a need for the development of potent and selective ATR inhibitors for the treatment of cancer, either as single agents or as combination therapies with radiotherapy or genotoxic chemotherapy.
SUMMARY OF THE INVENTION
[0006] The present invention relates to pyrazine compounds useful as inhibitors of ATR protein kinase. The invention also relates to pharmaceutically acceptable compositions comprising the compounds of this invention; methods of treating of various diseases, disorders, and conditions using the compounds of this invention; processes for preparing the compounds of this invention; intermediates for the preparation of the compounds of this invention; and methods of using the compounds in in vitro applications, such as the study of kinases in biological and pathological phenomena; the study of intracellular signal transduction pathways mediated by such kinases; and the comparative evaluation of new kinase inhibitors. These compounds have an unexpected ability to treat cancer as single agents. These compounds also show surprising synergy with other cancer agents, such as cisplatin, in combination therapies.
DETAILED DESCRIPTION OF THE INVENTION [0007] One aspect of this invention provides a compound of Formula IA:
Figure imgf000004_0001
IA or a pharmaceutically acceptable salt thereof; wherein Y is a C1-C1oaliphatic chain wherein up to three methylene units of the aliphatic chain are optionally replaced with O, NR0, S, C(O) or S(O)2; Ring A is a 5 membered heteroaryl ring selected from
Figure imgf000005_0001
J3 is H or C1-4alkyl wherein 1 methylene unit of the alkyl group can optionally be replaced with O, NH, N( C1-4alkyl), or S and optionally substituted with 1-3 halo;
Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R5 is H; a 3-7 membered monocyclic fully saturated, partially unsaturated, or aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; an 8-10 membered bicyclic fully saturated, partially unsaturated, or aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R5 is optionally substituted with 1-5 J5 groups;
L is a C1-4alkyl chain wherein up to two methylene units of the alkyl chain are optionally replaced with O, NR6, S, -C(O)-, -SO-, or -SO2-;
R0 is H or C1-C6alkyl wherein one methylene unit of the alkyl chain can be optionally replaced with O, NH, N(d.4alkyl), or S;
R1 is H or C1-C6alkyl;
R2 is H, C1-C6alkyl, -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said heterocyclic ring is optionally substituted with one occurrence of Jzι;
JZ1 is halo, CN, C1-C8aliphatic, -(X)t-CN, or -(X)r-Z; wherein said up to two methylene units of said C1-C8aliphatic can be optionally replaced with O, NR, S, P(O), C(O), S(O), or S(O)2; wherein said C1-C8aliphatic is optionally substituted with halo, CN, Or NO2;
X is C1-C4alkyl; each t, r and m is independently O or 1 ; Z is -NR3R4;
R3 is H or C1-C2alkyl;
R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said ring is optionally substituted with one occurrence of Jz;
R6 is H, or C1-C6alkyl;
Jz is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic), CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic;
J5 is halo, oxo, CN, NO2, X'-R, Or -(X1)p-Q4;
X1 is C1-10aliphatic; wherein 1-3 methylene units of said C1-10aliphatic are optionally replaced with -NR'-, -0-, -S-, C(=NR'), C(O), S(O)2, or S(O); wherein X1 is optionally and independently substituted with 1-4 occurrences of NH2, NH(C1 -4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO2(C1-4aliphatic), C(O)NH2, C(O)NH(C1-4aliphatic), C(O)N(C1-4aliphatic)2, SO(C1-4aliphatic), SO2(C1-4aliphatic), SO2NH(C1-4aliphatic), SO2NH( C1-4aliphatic), NHC(O)(C1-4aliphatic), N(C1-4aliphatic)C(O)(C1-4aliphatic), wherein said C1-4aliphatic is optionally substituted with 1-3 occurrences of halo;
Q4 is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 8-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q4 is optionally substituted with 1-5 JQ4;
JQ4 is halo, CN, or C1-4alkyl wherein up to 2 methylene units are optionally replaced with O, NR*, S, C(O), S(O), or S(O)2;
R is H or C1-4alkyl wherein said C1-4alkyl is optionally substituted with 1-4 halo;
J2 is halo; CN; a 5-6 membered aromatic or nonaromatic monocyclic ring having 0-3 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C1-10aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; wherein said C1-10aliphatic group is optionally substituted with 1-3 halo or CN; and said monocyclic ring is optionally substituted with 1-3 occurences of halo; CN; a C3-6cycloalkyl; a 3-7 membered heterocyclyl containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C1-4alkyl wherein up to one methylene unit of the alkyl chain is optionally replaced with O, NR", or S; and wherein said C1-4alkyl is optionally substituted with 1-3 halo; q is 0, 1 , or 2; p is 0 or 1 ;
R', R", and R* are each independently H, C1-4alkyl, or is absent; wherein said C1-4alkyl is optionally substituted with 1-4 halo.
[0008] In some embodiments,
Y is a C1-C6aliphatic chain wherein one methylene unit of the alkyl chain is optionally replaced with C(O) or -NR0-; and
R5 is a 3-7 membered monocyclic fully saturated, partially unsaturated, or aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic fully saturated, partially unsaturated, or aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R5 is
optionally substituted with 1-5 J groups, provided that when Ring A is
Figure imgf000007_0002
, p is 1 ; and R5 is aromatic.
[0009] In some embodiments, Ring A is a 5 membered heteroaryl ring selected from
Figure imgf000007_0001
[0010] In some embodiments, ring A is
Figure imgf000007_0003
[0011] In other embodiments, ring A is
Figure imgf000007_0004
[0012] It should be understood that Ring A structures can be bound to the pyrazine ring in two different ways: as drawn, and the reverse (flipped). For example, when Ring A is
Figure imgf000007_0005
it can be bound to the pyrazine ring as shown below:
Figure imgf000008_0001
or
Figure imgf000008_0002
"as drawn" "reversed"
[0013] Similarly, when Ring A is
Figure imgf000008_0003
, it can also be bound to the pyrazine ring in two ways - as drawn and reversed. In some embodiments, the Ring A structures are bound as drawn.
[0014] In other embodiments, J3 is H.
[0015] In yet other embodiments, Y is a C1^alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with -NR0-.
[0016] In some embodiments, J5 is a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O or NR'R" where each R' and R" is independently H or alkyl; or R' and R" taken together to form a 3-6 membered heterocyclic ring; NH2,
NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN,
CO2H, CO(C1-4aliphatic), CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic;
[0017] In other embodiments, J is halo, C1-C2alkyl optionally substituted with 1-3 fluoro, CN, or a C1-4alky! group wherein up to 2 methylene units are optionally replaced with S(O), S(O)2, C(O), or NR'. [0018] In yet another embodiment, Y is NH; R5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R5 is optionally substituted with 1-5 J5 groups; L is-C(O>- or -SO2-; R1 is H, or C1-C6alkyl; R2 is -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; Jzι- is -(X)1-CN, C1-C6alkyl or -(X) -Z; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said ring is optionally substituted with one occurrence of
Jz;
J5 is halogen, NO2, CN, O(haloC|_4aliphatic), haloC1 -4aliphatic, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'; and
J2 is halo, C1-C2alkyl optionally substituted with 1-3 fluoro, or CN. [0019] According to another embodiment, L is -C(O)-, m is 0, and R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms. In some embodiments, said heterocyclyl is pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl.
[0020] According to another embodiment, m is 0, q is 0, and -L-NR1R2 is C(O)pyrrolidinyl, C(O)piperidinyl, C(O)piperazinyl, C(O) azepanyl, C(O) 1,4-diazepanyl, C(0)NH-piperidinyl, C(0)NHCH2CH2-pyrrolidinyl, C(0)NHCH2CH2-piperidinyl, CON(CH3)CH2CH2N(CFb)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl or 1 ,4-diazepanyl is optionally substituted with C1-4alkyl or N(C1-4alkyl)2. [0021] According to yet another embodiment, J2 is halo; CN; phenyl; oxazolyl; or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN. [0022] Another embodiment provides a compound of Formula IA':
Figure imgf000010_0001
IA' or a pharmaceutically acceptable salt thereof; wherein
Y is a C1-C4alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with -NR0-; G is O or S; Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R5 is a 3-7 membered monocyclic fully saturated, partially unsaturated, or aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic fully saturated, partially unsaturated, or aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R5 is optionally substituted with 1-5 J5 groups; L is C1-4alkyl chain wherein up to two methylene units of the alkyl chain are optionally replaced with O, NR6, S, -C(O)-, -SO-, or -SO2-; R0 is H or C1-C6alkyl; R' is H or C1-C6alkyl; R2 is H, C1-Cόalkyl, -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen, sulfur, or oxygen; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)r-Z; X is C1-C4alkyl; each t, r and m is independently 0 or 1 ;
Z is -NR3R4;
R3 is H or C1-C2alkyl;
R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; R6 is H, or C1-C6alkyl; Jz is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH,
O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic), CO2(C1-4aliphatic),
O(haloC1-4aliphatic), or haloC1 -4aliphatic; J5 is halo, oxo, CN, NO2, X'-R, OT -(X 1)p-Q4,
X1 is C1-10aliphatic; wherein 1-3 methylene units of said C1-10aliphatic are optionally replaced with -NR'-, -0-, -S-, C(O), S(O)2, or S(O); wherein X1 is optionally and independently substituted with 1-4 occurrences of NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O( C1-4aliphatic), NO2, CN, CO2H, CO2( C1-4aliphatic), C(O)NH2, C(O)NH(C1-4aliphatic), C(O)N(C1-4aliphatic)2, SO( C1-4aliphatic), SO2(C1-4aliphatic), SO2NH(C1-4aliphatic), SO2NH(C1-4aliphatic), NHC(O)(C1-4aliphatic), N(C1-4aliphatic)C(O)(C1-4aliphatic), wherein said C1-4aliphatic is optionally substituted with 1-3 occurrences of halo;
Q4 is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 8-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q4 is optionally substituted with 1-5 JQ4;
JQ4 is halo, CN, or C1-4alkyl wherein up to 2 methylene units are optionally replaced with O, NR*, S, C(O), S(O), or S(O)2;
R is H or C1-4alkyl wherein said C1-4alkyl is optionally substituted with 1-4 halo;
J2 is halo; CN; a 5-6 membered aromatic or nonaromatic monocyclic ring having 0-3 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C1-10aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; wherein said C1-10aliphatic group is optionally substituted with 1-3 halo or CN; and said monocyclic ring is optionally substituted with 1-3 halo; CN; a C3-6cycloalkyl; a 3-7 membered heterocyclyl containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C1-4alkyl wherein up to one methylene unit of the alkyl chain is optionally replaced with O, NR", or S; R', R", and R* are each independently H, C1-4alky!, or is absent; wherein said C1-4alkyl is optionally substituted with 1-4 halo, q is 0, 1, or 2, p is 0 or 1.
[0023] In some embodiments, J5 is halogen, NO2, CN, 0(haloC1-4aliphatic), haloC1-4aliphatic, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'. In other embodiments, J5 is halo, CN, phenyl, oxazolyl, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR', C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN.
[0024] In yet other embodiments, J2 is halo; CN; phenyl; oxazolyl; or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN. [0025] In some embodiments, Y is a C1-C2alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with NR0.
[0026] In some embodiments, p is 0 and Q is phenyl, indolyl, pyridyl, naphthyl or benzothienyl, or quinolinyl. In certain embodiments, Q is phenyl, indolyl, pyridyl, or quinolinyl. In some embodiments, Q is phenyl or pyridyl. In some embodiments, phenyl. In other embodiments, pyridyl.
[0027] J2 is -OCH3, -OCH2CH2N(CH3)2, -NHCH2CH2N(CH3)2, or piperazinyl. [0028] The compound according to claim 19, wherein Q is substituted in the ortho position, the para position, or in both the ortho and the para position. [0029] The compound according to claim 19, wherein Q is substituted at the para position with J2, wherein the J2 is a C1-6aliphatic group wherein the methylene group bonded to Ring Q is replaced with -SO2-. [0030] The compound of claim 19 wherein at least one more methylene unit of the C1-6aliphatic group is optionally replaced with a heteroatom selected from the group consisting of O, NR", and S.
[0031] The compound according to claim 19, wherein Q is substituted at the para position with -SO2(C1-4alkyl), -SO2(C1-4alkyl)N(C1-4alkyl)2, C(O)N(C1-4alky I)2,
C(O)(1,4-diazepanyl), CO(azepanyl), C(O)(piperazinyl), or C(O)(piperidinyl).
[0032] The compound of claim 21, wherein at least one more methylene unit of the C1-6aliphatic group is optionally replaced with a heteroatom selected from the group consisting of O, NR", and S.
[0033] In some embodiments, Q is optionally substituted in the ortho-position with one
J2, wherein J2 is C1-C4alkyl, NH2, NHC(O)CH3, O(C1-C4alkyl), CH2OH, CH2OCH3,
CH2CH2OCH3, CH2CN, CN, CH2C(O)NH2, OH, OCF3, CF3, CHF2, -CH=CHF,
NHCOCH3, COCH3, CONH2, SCH3, SOCH3, SOCH2CH3, SO2CH(CH3)2, -C≡≡CH, oxazolyl, or phenyl. In some embodiments, J2 is substituted in the ortho position with
CH2OH, CHF2, S(O)CH3, or S(O)CH2CH3.
[0034] In yet other embodiments, Q is optionally substituted in the ortho-position with J2, wherein J2 is C1-4alkyl, -C≡C-(C1-4alkyl), CH=CH2, CH=CHF, O(C1-4alkyl), NH(C1-4alkyl),
N(C1-4alkyl)2, -(C1-4alkyl)OH, -(C1-4alkyl)O(C1-4alkyl), -(C1-4alkyl)NH2, -(C1-4alky I)NH(C1-
4alkyl), -(C1-4alkyl)N(C1-4alkyl)2, -(C1-4alky I)CN, CO(C1-4alkyl), CON(C1-4alkyl)2,
C(O)O(C1-4alkyl), S(C1-4alkyl), -S-(C1-4alky I)NH2, S(O)(C1-4alky I)NH2, S(O)2(C1-4alkyl)OH,
S(O)(C1-4alkyl)NHC(O)O(t-butyl), NHS(O)2(C1-4alkyl), halo, or CN.
[0035] In some embodiments, J2 is CH2CH2OH, SCH(CH3)2, -C≡≡CCH3, halo, CN,
CON(CH3)2, CH2CN, S(O)CH2CH2NH2, SCH2CH2NH2, C(O)OCH3, CH2N(CH3)2,
S(O)CH2CH2NHBOC, N(CH3)2, NHSO2CH3, CH=CHF, CH2OCH3, CH=CH2, SCH2CH3, or
-CH=CH.
[0036] In other embodiments, Q is optionally substituted in the para position with J2, wherein J2 is -SO2(C1-4alkyl), -SO2(C3-6cycloalkyl), -SO2(3-6 membered heterocyclyl),
-SO2(C1-4alkyl)N(C1-4alkyl)2, -C(O)(C1-4alkyl), -(C1-4alkyl)N(C1-4alkyl)2, or -NHC(0)(C1-
4alkyl).
[0037] In some embodiments, said 3-6 membered heterocyclyl is tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, or piperidinyl. [0038] In yet other embodiments, Q is optionally substituted in the meta position with J2 wherein J2 is C1-4alkyl, C1-4alkoxy, halo, haloC1-4alkyl, haloC1-4alkoxyl, CN, SO2(C1-4aIkyl),
NHSO2(C,-4alkyl), C(O)(C1-4alkyl), C(O)NH2, NHC(O)(C1-4alkyl), -(C1-4alky I)-OH,
-(C1-4alkyl)-O(C,-4alkyI), -(C1-4alky I)-NH2, -(C1-4alkyl)-N(C1-4alkyl)2, or
-(C1-4alkyl)NH(C1-4alkyl).
[0039] In some embodiments, Q is naphthyl or benzothienyl.
[0040] In another embodiment, Q is pyridyl. In some embodiments, Q is substituted in the ortho-position with one J2, wherein J2 is CN.
[0041] In some embodiments, Q is substituted with one or two J2, wherein J2 is a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O or NR".
[0042] In some embodiments, J2 is -OCH3, -OCH2CH2N(CH3)2, -NHCH2CH2N(CH3)2, or piperazinyl.
[0043] In another embodiment, p is 1. In some embodiments, Q is phenyl, pyridyl, or naphthyl. In some embodiments, said pyridyl is 3-pyridyl or 4-pyridyl. In other embodiments, Q is phenyl.
[0044] In some embodiments, Q comprises Q1 and optionally Q2 as shown in formula
IA-i, wherein Q1 is a six membered ring and -LNR1R2 is substituted in the para-position as shown below:
Figure imgf000014_0001
IA-i
[0045] In some embodiments, J5 is halogen, NO2, CN, O(haloC1-4aliphatic), haloC1-4aliphatic, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'.
[0046] In some embodiments, Q1 is phenyl or pyridyl. In other embodiments, Q'-Q2 is naphthyl. [0047] In some embodiments, Y is a C1-C2alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with NR0.
[0048] In other embodiments, L is -C(O)- or -SO2-.
[0049] In yet other embodiments, R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen, sulfur, or oxygen. In some embodiments, said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, 1,4-diazepanyl, or 1 ,4-oxazepanyl. In yet other embodiments, R1 is C1-C6alkyl. In some embodiments, said heterocyclic ring is optionally substituted with halo, CN, C1 ^aliphatic, haloC1-6aliphatic, -C(O)O(C1-6aliphatic),
C(O)H, C(O)(C1-6aliphatic), P(O)(OC1-4alkyl)2, NH(Cwaliphatic), orN(C1-6aliphatic)2.
[0050] In some embodiments, R2 is C1-C6alkyl. In other embodiments, R2 is
-(C2-C6alkyl)-Z.
[0051] According to another embodiment, m is 0.
[0052] According to yet another embodiment, q is 0.
[0053] In some embodiments, L is -C(O)-.
[0054] In some embodiments, R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms. In some embodiments, said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl. In other embodiments, said heterocyclic ring is selected from
Figure imgf000015_0001
[0055] In some embodiments, t is 1. In other embodments, t is 0.
[0056] In other embodiments, R1 is H or C1-C6alkyl; and R2 is -(C2-C6alkyl)-Z. In some embodiments, R1 is C1-C6alkyl. In some embodiments, Z is -NR3R4, wherein R3 and R4 are both C1-C2alkyl. In other embodiments, R3 and R4, taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or
1,4-diazepanyl. In some embodiments, said ring is pyrrolidinyl or piperidinyl.
[0057] In some embodiments, said ring is optionally substituted with one JZ1. In some embodiments, JZ1 is (X)r-Z. In other embodiments, JZ1 is C1-4alkyl or N(C1-4alkyl)2.
[0058] In one embodiment, p is 0, q is 0, and -L-NR1R2 is C(O)pyrrolidinyl,
C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O) 1,4-diazepanyl, C(O)NH-piperidinyl, C(O)NHCH2CH2-pyrrolidinyl, C(O)NHCH2CH2-piperidinyl, CON(CH3)CH2CH2N(CH3)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl is optionally substituted with C1-4alkyl or N(C1-4alkyl)2. In one embodiment, -L-NR1R2 is C(O)1 ,4- di azepanyl.
[0059] According to another aspect, m is 0. In some embodiments, R5 is thienyl, thiazolyl, furanyl, pyrrolidinyl, azetidinyl, piperidinyl, piperazinyl, morpholinyl, pyridinonyl, pyridyl, tetrahydropyridinyl, tetrahydroisoquinolinyl, 1,4-diazepanyl, azabicyclo[2.2.1]heptanyl, or phenyl. In other embodiments, R5 is phenyl, piperidinyl or thienyl. In some embodiments, Q is optionally substituted in the para position with -SO2(C1-
4alkyl), -SO2(C1-4alkyl)N(C1-4alkyl)2, C(O)N(C1-4alkyl)2, C(O)( 1 ,4-diazepanyl),
C(O)(piperazinyl), or C(O)(piperidinyl).
[0060] According to another aspect, R5 is phenyl. In some embodiments, R5 is optionally substituted with 1-2 J5 groups, wherein J5 is selected from halo, CN, NO2, X'-R, or -(X')p-Q4; p is 0-1 ; X1 is a C1-10aliphatic wherein 1 -3 methylene units of said C1-6aliphatic are optionally replaced with -NR'-, -O-, -S-, C(=NH)-, C(O), S(O)2, or S(O); R is H; and Q4 is a 3-6 membered monocyclic ring containing 0-2 heteroatoms selected from O or N, wherein X1 is optionally substituted with 1-3 occurrences of halo or CN.
[0061] In other embodiments, J5 is a C1-10aliphatic chain wherein 1-2 methylene units of
X1 are replaced with -O- or -NR'-.
[0062] According to another aspect, R5 is piperidinyl optionally substituted with NH2 or
-(C1-4alkyl)NH2. According to yet another aspect, R5 is thienyl optionally substituted with
CN, C1-6alkyl, -(C1-4alky I)NH2, -(C1-4alkyl)NH(C1-6alkyl), -(C1-4alky I)N(C1-6alkyl)2, 0(C1-
6alkyl), pyrrolidinyl, wherein said alkyl is optionally substituted with 1-3 halo.
[0063] In some embodiments, Q4 is an optionally substituted 3-6 membered cycloalkyl ring. In other embodiments, Q4 is an optionally substituted 3-6 membered heterocyclic ring selected from pyrrolidinyl, azetidinyl, or thienyl.
[0064] In some embodiments, J5 is halo, C1-6alkyl, NO2, CN, C1-6aikyl, -CH=CH2, OH,
OCH3, OCH2CH3, OCH(CH3)2, NH2, CH2NH2, CH2OH, CH(CH3)NHCH3, C(CH3)2NH2,
CH2CH2NH2, CH2CH2OH, CH2NHCH3, CH2N(CH3)2, CH(CH3)NH2,
CH(CH3)NHC(O)O(CH3)3, CH2NHC(CH3)2, CH2NHCH2CHF2, CH2NHCH2CH(CH3)OH,
CH2NHCH2C(CH3)2OH, CH2NHCH2CH(OH)-cyclopropyl, CH2NHCH2CH2N(CH3)2, CH2NHCH(CH2CH3)S, CH2NHCH3, CH2NHCH2CH3, CH2NHCH2CH2CH3, CH2NH- cyclopropyl, CH2NHCH2CH2OH, CH2NHCH2CH2OCH3, CH2NHCH2CH2OCH2CH2OH, azetidinyl, pyrrolidinyl, CF3, C(=NH)NH2, C(=NH)NH(CH3),thienyl, CH2NH-cyclopropyl,
CH2NH(CH2OH)3, OCH2CH2OH, OCH2CH2CH2OH, OCH2CH2NHC(O)OC(CH3)3,
CH2NHC(O)O(CH3)3, or CH2OC(O)CH3.
[0065] According to another aspect, m is 1.
[0066] In some embodiments, R5 is H.
[0067] In some embodiments, Y is -NH-, -NHCH2-, -NHC(O)-, C(O)NH, C(O)NHCH2,
C(O), -NHCH(CH3)- or -N(CH3)CH2-; and R5 is phenyl. In some embodiments, R5 is optionally substituted with halo or C1-4alkyl, wherein up to 1 methylene unit is optionally replaced with O, NR', or S.
[0068] Another embodiment provides a compound of Formula HA:
Figure imgf000017_0001
IIA or a pharmaceutically acceptable salt thereof; wherein Ring A is a 5 membered heteroaryl ring selected from
Figure imgf000017_0002
or
Figure imgf000017_0003
Y is a C 1-C4alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with -NR0-; Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R5 is optionally substituted with 1-5 J5 groups; L iS-C(O)- Or -SO2-; R1 is H, or C1-C6alkyl; R0 is H or C1-C6alkyl; R2 is C1-C6alkyl, -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen, sulfur, or oxygen; wherein said heterocyclic ring is optionally substituted with one occurrence of Jzι; JZ1 is (X)t-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, r and m is independently 0 or 1 ; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; Jz is NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic),
NO2, CN, CO2H, CO(C1-4aliphatic), CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; J5 is halogen, NO2, CN, O(haloC1-4aliphatic), haloC1-4aliphatic, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'; J2 is halo; CN; phenyl; oxazolyl; or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN; R' and R" are each independently H or C1-C4 alkyl; q is O, I, or 2, p is O or 1. [0069] In some embodiments, Q is phenyl or pyridyl.
[0070] In other embodiments, Y is a C1-C2alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with NR0.
[0071] Another embodiment provides a Compound from Table IIA-2:
Table IIA-2
Figure imgf000019_0001
Figure imgf000020_0001
nother embodiment provides a compound of Formula HIA:
Figure imgf000021_0001
IIIA or a pharmaceutically acceptable salt thereof; wherein
Y is a C1-C4alkyl chain wherein one methylene unit of the alky I chain is optionally replaced with -NR0-; Q is phenyl or pyridyl; R5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R is optionally substituted with 1-5 J5 groups; L iS-C(O)- Or -SO2-; R1 is H, or C1-C6alkyl; R° is H or C1-C6alkyl; R2 is C1-C6alkyl, -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen, sulfur, or oxygen; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)1-CN, C|-C6alkyl or -(X) -Z; X is C1-C4alkyl; each t, r and m is independently 0 or 1 ; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; Jz is NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, 0(C1-4aliphatic),
NO2, CN, CO2H, CO(C1-4aliphatic), CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; J5 is halogen, NO2, CN, O(haloC| -4aliphatic), haloC1-4aliphatic, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'; J2 is halo; CN; phenyl; oxazolyl; or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN; R' and R" are each independently H or C1-C4 alkyl; q is O, I, or 2, p is O or 1.
[0073] In some embodiments, Y is a C1-C2alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with NR0.
[0074] In other embodiments, p is O and Q is pyridyl. In some embodiments, m is O. [0075] In yet other embodiments, R5 is phenyl or thienyl. In some embodiments, R5 is phenyl optionally substituted with one occurrence OfNH2, C1-C4alkyl, or CH2NH2. [0076] Another embodiment provides a compound selected from Table IHA:
Table IHA
Figure imgf000022_0001
Figure imgf000023_0002
[0077] Another aspect provides a compound of formula IA-ii:
Figure imgf000023_0001
IA-ii or a pharmaceutically acceptable salt thereof; wherein
Y is NH;
Ring A is a 5 membered heteroaryl ring selected from
R5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R5 is optionally substituted with 1 -5 J5 groups;
L iS -C(O)- Or -SO2-;
R1 is H, or C1-C6alkyl;
R" is -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of
Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)r-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, r and m is independently 0 or 1 ; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; each Jz, J1, and J5 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic),
CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; J2 is halo, C1-C2alkyl optionally substituted with 1-3 fluoro, or CN; q is O, I, or 2. [0078] According to one embodiment, Ring A is or
Figure imgf000025_0002
Figure imgf000025_0003
[0079] According to another embodiment, m is 0.
[0080] According to another embodiment, q is 0.
[0081] In some embodiments, L is -C(O)-.
[0082] In some embodiments, R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms. In some embodiments, said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl. In other embodiments, said heterocyclic ring is selected from
Figure imgf000025_0001
[0083] In some embodiments, t is 1. In other embodments, t is 0. [0084] In other embodiments, R1 is H or C1-C6alkyl; and R2 is -(C2-C6alkyl)-Z. In some embodiments, R1 is C1-Cδalkyl. In some embodiments, Z is -NR3R4, wherein R3 and R4 are both C1-C2alkyl. In other embodiments, R3 and R4, taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl. In some embodiments, said ring is pyrrolidinyl or piperidinyl. [0085] In some embodiments, said ring is optionally substituted with one JZ1. In some embodiments, JZ1 is (X)r-Z. In other embodiments, JZ1 is C1-4alkyl or N(C1-4alkyl)2. [0086] In one embodiment, p is 0, q is 0, and -L-NR1R2 is C(O)pyrrolidinyl, C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O) 1,4-diazepanyl, C(O)NH-piperidinyl, C(O)NHCH2CH2-pyrrolidinyl, C(O)NHCH2CH2-piperidinyl, CON(CH3)CH2CH2N(CH3)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl is optionally substituted with C1-4alkyl or N(C1-4alkyl)2. In one embodiment, -L-NR1R2 is C(O)1 , 4- diazepanyl. [0087] Another embodiment provides a compound selected from Table IA:
Table IA
Figure imgf000026_0001
IA-I IA-2
[0088] Another embodiment provides a compound of Formula IA-iii: 5 P
Figure imgf000026_0002
IA-
Figure imgf000026_0006
iii: or a pharmaceutically acceptable salt thereof wherein;
Ring A is
Figure imgf000026_0004
or
Figure imgf000026_0005
J5O is H, F, Cl, C1-4aliphatic, O(C1-3aliphatic), or OH;
J
Figure imgf000026_0003
P is P2 ;
J5pl is H, C1-4aliphatic, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl; wherein J5p2 is optionally substituted with 1-2 occurrences of OH or halo; J5p2 is H, methyl, ethyl, CH2F, CF3, or CH2OH; J2O is H, CN, or SO2CH3; J2m is H, F, Cl, or methyl; J2P is -SO2(C1-6alkyl), -SO2(C3-6cycloalk;yl), -SO2(4-6 membered heterocyclyl),
-SO2(C1-4alkyl)N(C1-4alkyl)2, or -SO2(C1-4alkyl)-(4-6 membered heterocyclyl), wherein said heterocyclyl contains 1 heteroatom selected from the group consisting of O, N, and S; and wherein said J2p is optionally substituted with 1-3 occurences halo, OH, or O(C1-4alkyl).
[0089] In some embodiments, Ring A is
Figure imgf000027_0001
[0090] In other embodiments, Ring A is
Figure imgf000027_0002
[0091] Another embodiments provides a compound from one of the following Tables:
Table IA-2
Figure imgf000027_0003
Figure imgf000028_0001
Figure imgf000029_0001
Figure imgf000030_0001
Figure imgf000031_0001
Figure imgf000032_0001
Figure imgf000033_0001
Figure imgf000034_0001
Table IA-3
Figure imgf000034_0002
Figure imgf000035_0001
Figure imgf000036_0001
Figure imgf000037_0001
Figure imgf000038_0001
Figure imgf000039_0001
Figure imgf000040_0001
Figure imgf000041_0001
Figure imgf000042_0001
Figure imgf000043_0001
Figure imgf000044_0001
Figure imgf000045_0001
Figure imgf000046_0001
Figure imgf000047_0001
Figure imgf000048_0001
Figure imgf000049_0001
Figure imgf000050_0001
Figure imgf000051_0001
Figure imgf000052_0001
Figure imgf000053_0001
Figure imgf000054_0001
Figure imgf000055_0001
Figure imgf000056_0001
Figure imgf000057_0001
Figure imgf000058_0001
Figure imgf000059_0001
Figure imgf000060_0001
Figure imgf000061_0001
Figure imgf000062_0001
Figure imgf000063_0001
Figure imgf000064_0001
Figure imgf000065_0001
Table IVA
Figure imgf000066_0002
Table IA-4 (part 1)
Figure imgf000066_0001
Figure imgf000067_0001
Figure imgf000068_0001
Figure imgf000069_0001
Figure imgf000070_0001
Figure imgf000071_0001
Figure imgf000072_0001
Figure imgf000073_0002
Table IA-4 (part 2)
Pl P3
P6
Figure imgf000073_0001
Figure imgf000074_0001
Figure imgf000075_0001
Figure imgf000076_0001
P43 P45
P52 P54
Figure imgf000077_0001
Figure imgf000078_0001
Figure imgf000079_0001
Figure imgf000080_0001
Figure imgf000081_0001
Figure imgf000082_0001
Figure imgf000083_0001
Figure imgf000084_0001
P136 P138
Figure imgf000085_0001
92] Another aspect provides a compound of formula II,
Figure imgf000086_0001
or a pharmaceutically acceptable salt thereof; wherein
Ring A is a 5-6 membered monocyclic aromatic ring containing 0-2 heteroatoms selected from N, O, or S; Ring A is optionally fused to a 5-6 membered aromatic ring containing
0-2 heteroatoms selected from N, O, or S; L is -C(O)-; R1 is C1-Cealkyl; R2 is -{C2-C6alkyl)-Z or a 4-8 membered heterocyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, p, and r is independently 0 or 1 ; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; each Jz and J1 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO2 (C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic;
J2 is halo, C1-C2alkyl optionally substituted with 1-3 fluoro, or CN; q is O, I , or 2.
[0093] In some embodiments, when Ring A is
Figure imgf000087_0003
, and R1 is H; then R2 is not
Figure imgf000087_0001
[0094] In some embodiments, Ring A is a 6-membered ring not fused to another ring. In other embodiments, Ring A is phenyl, pyridyl, or pyrimidyl. In yet other embodiments, Ring A is phenyl.
[0095] In some embodiments, R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms. In some embodiments, said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl. In other embodiments, said heterocyclic ring is selected from
Figure imgf000087_0002
[0096] In yet other embodiments, the ring formed by R1 and R2 is optionally substituted with CH2pyrrolidinyl, C1-4alkyl, N(C1-4alkyl)2, or CH2CH2CN.
[0097] In some embodiments, t is 1. In other embodiments, t is 0.
[0098] In other embodiments, R1 is H or C1-C6alkyl; and R2 is -(C2-C6alkyl)-Z.
[0099] In some embodiments, Z is -NR3R4, wherein R3 and R4 are both C1-C2alkyl. In other embodiments, R3 and R4, taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl. In some embodiments, said ring is pyrrolidinyl or piperidinyl.
[00100] In some embodiments, said ring is optionally substituted with one Jz. In some embodiments, Jz is C1-4alkyl or N(C1-4alkyl)2.
[00101] In one embodiment, p is 0, q is 0, and -L-NR1R2 is C(0)pyrrolidinyl,
C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O)1 , 4-diazepanyl,
CON(CH3)CH2CH2N(CH3)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl is optionally substituted with CH2pyrrolidinyl, C1-4alkyl, N(C1-4alkyl)2, or CH2CH2CN.
[00102] Another embodiment provides a compound selected from Table II.
Table II
II-3
11-4 II-6
Figure imgf000088_0001
II-7 II-9
Figure imgf000089_0001
11-10 IM l.
[00103] Another aspect provides a compound of formula III:
Figure imgf000089_0002
or a pharmaceutically acceptable salt thereof; wherein
L is -C(O)- or -SO2-;
R' is H, or C1-C6alkyl;
R2 is -(C2-C6alkyl)-Z or a 4-8 membered heterocyclic ring containing 0-2 nitrogen atoms;,' wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of Jzι; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, p, and r is independently 0 or 1 ; Z is -NR3R4;
R3 is H or C1-C2alkyl;
R4 is H or C1-C6alkyI; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; each Jz and J1 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic),
CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1 -4aliphatic; J2 is halo, C1-C2alkyl optionally substituted with 1-3 fluoro, or CN; q is O, I, or 2.
Figure imgf000090_0001
Ill-i
[00104] In some embodiments, J1 is Jla or Jlb as depicted in Formula Ill-i. [00105] In some embodiments, when L is -C(OK <\ is 0, Jla is H, and Jl b is H or F; then: when R1 is H; then R2 is not -(C1-4alkyl)-N(CH3)2; or
R1 and R taken together are not
Figure imgf000090_0003
[00106] In some embodiments, R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms. In some embodiments, said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl. In other embodiments, said heterocyclic ring is selected from
Figure imgf000090_0002
[00107] In some embodiments, t is 1. In other embodments, t is 0. [00108] In other embodiments, R1 is H or C1-C6alkyl; and R2 is -(C2-C6alkyl)-Z. In some embodiments, Z is -NR3R4, wherein R3 and R4 are both C1-C2alkyl. In other embodiments, R3 and R4, taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl. In some embodiments, said ring is pyrrolidinyl or piperidinyl.
[00109] In some embodiments, said ring is optionally substituted with one Jz. In some embodiments, Jz is C1-4alkyl or N(C1-4alkyl)2.
[00110] In one embodiment, p is 0, q is 0, and -L-NR1R2 is C(O)pyrrolidinyl, C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O)1, 4-diazepanyl, C(O)NH-piperidinyl, C(O)NHCH2CH2-pyrrolidinyl, C(O)NHCH2CH2-piperidinyl, CON(CH3)CH2CH2N(CH3)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl is optionally substituted with C1-4alkyl or N(C1-4alkyl)2.
[00111] Another embodiment provides a compound selected from Table III:
Table III
Figure imgf000091_0001
Figure imgf000091_0002
Figure imgf000092_0002
other aspect provides a compound selected from Table III-2:
Table III-2
Figure imgf000092_0001
Figure imgf000092_0003
Figure imgf000093_0001
Figure imgf000094_0001
Figure imgf000095_0001
[00113] Another aspect provides a compound of Formula V:
Figure imgf000096_0001
V or a pharmaceutically acceptable salt thereof: wherein Ring A is a 8-9 membered bicyclic heteroaryl ring having 1-4 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur; Q is a 5-6 membered monocyclic aromatic ring containingO-3 heteroatoms independently selected from the group consisting of nitrogen, oxygen, and sulfur; L is C1-4alkyl chain wherein up to two methylene units of the alkyl chain are optionally replaced with O, NR6, S, -C(O)-, -SO-, or -SO2-; R' is H or C1-C6alkyl; R2 is H, C1-C6alkyl, -(C2-C6alkyl)-Z, or a 3-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 3-8 membered monocyclic or 8-9 membered bicyclic heterocyclic ring containing 1-2 heteroatoms selected the group consisting of oxygen, nitrogen, and sulfur; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)r-Z1;. X is C1-4alkyl; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; Z1 is -NR5R6; R5 is H or C1-C2alkyl; R6 is H or C1-C6alkyl; or R5 and R6, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of JZ1; J1 is halo, CN, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN; J2 is halo; CN; or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN; each Jz and JZ1 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic),
CO2(C1-6aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; each q and m is independently O, 1 , or 2; each t, p, and r is independently O or 1.
[00114] According to one embodiment, Ring A is a 9-membered ring. In some embodiments, Ring A is a 5-6 bicyclic ring system. A 5-6 bicyclic system is a five- membered ring fused to a six membered ring as shown below.
Figure imgf000097_0001
[00115] Examples of 5-6 bicyclic systems include, but are not limited to, benzimidazolyl, benzoxazolyl, indazolyl, pyrrolopyridinyl, pyrrolopyrimidinyl, pyrrolopyrazinyl, benzothiazolyl, benzothiophenyl, indolyl, benzofuranyl, benzotriazolyl, and azaindolyl. [00116] In some embodiments, Ring A has 1-2 heteroatoms. In some embodiments, Ring A is benzimidazolyl, benzoxazolyl, indazolyl, benzothiazolyl, indolyl, benzotriazolyl, or azaindolyl. [00117] According to another embodiment, Ring Q is phenyl or pyridyl. In some embodiment, Q is phenyl.
[00118] In some embodiments, p is 1 and Ring Q is substituted in the para position with
L-NR • I o R2 as shown in formula V-a:
Figure imgf000098_0001
V-a
[00119] In some embodiments, L is C(O) or S(O)2. In other embodiments, R1 and R2 are both C1-4alkyl. In yet other embodiments, R1 and R2, taken together with the atom to which they are bound, form a 4-7 membered heterocyclic ring containing 1-2 nitrogen atoms. In some embodiments, said heterocyclic ring is selected from the group consisting of pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, and 1 ,4-diazepanyl. In some embodiments, said heterocyclyl is 1,4-diazepanyl. [00120] In other embodiments, Ring Q is pyridyl.
[00121] In some embodiments, p is 0. In other embodiments, q is 1 and J2 is CN. [00122] Another embodiment provides a compound selected from Table V:
Table V
Figure imgf000098_0002
Figure imgf000099_0001
Table V-2
Figure imgf000100_0001
Figure imgf000100_0002
Figure imgf000101_0001
Figure imgf000102_0003
Figure imgf000102_0002
or V-30. [00123] Another embodiment provides a compound of formula VI:
Figure imgf000102_0001
VI or a pharmaceutically acceptable salt thereof; wherein Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; L iS -C(O)- Or -SO2-; G is S or O; R1 is H, or C1-C6alkyl; R2 is -{C2-C6alkyl)-Z or a 4-8 membered heterocyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, p, and r is independently 0 or 1 ; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; each Jz and J1 is independently NH2, NH(C1-4aliphatic), N(C1-4aIiphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic),
CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; J2 is halo, C1-C2alkyl optionally substituted with 1-3 fluoro, or CN; q is O, I , or 2; p is O or 1.
[00124] According to one aspect of the invention, p is 1. In some embodiments, Q is phenyl. In other embodiments, L is -C(O)-. In some embodiments, R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1 -2 nitrogen atoms. In some embodiments, the heterocyclic ring formed by R1 and R2 is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl. In other embodiments, said heterocyclyl is
Figure imgf000103_0001
[00125] According to another aspect of the invention, p is 0, q is 0, and -L-NR1R2 is
C(O)1 , 4-diazepanyl.
[00126] Another embodiment provides a compound selected from Table VI: Table VI
Figure imgf000104_0001
Figure imgf000104_0003
[00127] Another embodiment provides a compound of formula VII,
Figure imgf000104_0002
or a pharmaceutically acceptable salt thereof; wherein
Ring A is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said ring is optionally substituted with J1; R1 is C1-C6alkyl; J1 is a C1-6 alkyl chain wherein 1-2 methylene units are optionally replaced with O, NR*, S, or C(O); J1 is optionally substituted with 1-3 occurrences of halo; R* is H or C1-4alkyl; JJ22 iiss hhaalloo,, CC,i--CC22aallkkyyll optionally substituted with 1-3 fluoro, or CN; Each p and q is independently 0, 1 , or 2.
[00128] According to one aspect of the invention, Ring A is a 5-6 membered heteroaryl having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur. In some embodiments ,Ring A is pyridinyl, pyrimidyl, pyrazinyl, triazinyl, pyrrolyl, pyrazolyl, triazolyl, thienyl, thiazolyl, thiadiazolyl, furanyl, oxazolyl, or oxadiaozolyl. In other embodiments, Ring A is pyridinyl, pyrazolyl, thiadiazolyl, or thiazolyl wherein Ring A is optionally substituted with halo or C1-4alkyl. In some embodiments, Ring A is phenyl. In some embodiments, said phenyl is substituted with one occurrence of J1. [00129] In some embodiments, J1 is a C1-6 alkyl chain wherein 1 methylene unit is replaced with N or O. In other embodiments, J1 is O(C1-4alkyl) or -(C1-4aIkyl)NH(C1-4alkyl). In yet other embodiments, J1 is -(C1-4alkyl)NH(C1-4alkyl).
[00130] Another embodiment provides a compound selected from the following:
Table VII
Figure imgf000105_0001
Figure imgf000106_0001
Figure imgf000107_0001
[00131] Another embodiment provides a compound selected from Table 1.
Table I
Figure imgf000108_0001
Figure imgf000109_0001
Figure imgf000110_0001
Figure imgf000111_0001
Figure imgf000112_0001
Figure imgf000113_0001
Figure imgf000114_0001
Figure imgf000115_0001
Figure imgf000116_0001
Figure imgf000117_0001
Figure imgf000118_0001
[00132] In some embodiments, the variables are as depicted in the compounds of the disclosure including compounds in the tables above.
[00133] Compounds of this invention include those described generally herein, and are further illustrated by the classes, subclasses, and species disclosed herein. As used herein, the following definitions shall apply unless otherwise indicated. For purposes of this invention, the chemical elements are identified in accordance with the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics, 75th Ed. Additionally, general principles of organic chemistry are described in "Organic Chemistry", Thomas Sorrell, University Science Books, Sausalito: 1999, and "March's Advanced Organic Chemistry", 5th Ed., Ed.: Smith, M.B. and March, J., John Wiley & Sons, New York: 2001, the entire contents of which are hereby incorporated by reference.
[00134] As described herein, a specified number range of atoms includes any integer therein. For example, a group having from 1-4 atoms could have 1, 2, 3, or 4 atoms. [00135] As described herein, compounds of the invention may optionally be substituted with one or more substituents, such as are illustrated generally herein, or as exemplified by particular classes, subclasses, and species of the invention. It will be appreciated that the phrase "optionally substituted" is used interchangeably with the phrase "substituted or unsubstituted." In general, the term "substituted", whether preceded by the term "optionally" or not, refers to the replacement of hydrogen radicals in a given structure with the radical of a specified substituent. Unless otherwise indicated, an optionally substituted group may have a substituent at each substitutable position of the group, and when more than one position in any given structure may be substituted with more than one substituent selected from a specified group, the substituent may be either the same or different at every position. Combinations of substituents envisioned by this invention are preferably those that result in the formation of stable or chemically feasible compounds.
[00136] Unless otherwise indicated, a substituent connected by a bond drawn from the center of a ring means that the substituent can be bonded to any position in the ring. In example i below, for instance, J1 can be bonded to any position on the pyridyl ring. For bicyclic rings, a bond drawn through both rings indicates that the substituent can be bonded from any position of the bicyclic ring. In example ii below, for instance, J1 can be bonded to the 5-membered ring (on the nitrogen atom, for instance), and to the 6-membered ring.
Figure imgf000119_0001
Figure imgf000119_0002
i ii
[00137] The term "stable", as used herein, refers to compounds that are not substantially altered when subjected to conditions to allow for their production, detection, recovery, purification, and use for one or more of the purposes disclosed herein. In some embodiments, a stable compound or chemically feasible compound is one that is not substantially altered when kept at a temperature of 40°C or less, in the absence of moisture or other chemically reactive conditions, for at least a week.
[00138] The term "aliphatic" or "aliphatic group", as used herein, means a straight-chain (i.e., unbranched), branched, or cyclic, substituted or unsubstituted hydrocarbon chain that is completely saturated or that contains one or more units of unsaturation that has a single point of attachment to the rest of the molecule.
[00139] Unless otherwise specified, aliphatic groups contain 1-20 aliphatic carbon atoms. In some embodiments, aliphatic groups contain 1-10 aliphatic carbon atoms. In other embodiments, aliphatic groups contain 1-8 aliphatic carbon atoms. In still other embodiments, aliphatic groups contain 1-6 aliphatic carbon atoms, and in yet other embodiments aliphatic groups contain 1-4 aliphatic carbon atoms. Aliphatic groups may be linear or branched, substituted or unsubstituted alkyl, alkenyl, or alkynyl groups. Specific examples include, but are not limited to, methyl, ethyl, isopropyl, n-propyl, sec-butyl, vinyl, n-butenyl, ethynyl, and tert-butyl.
[00140] The term "cycloaliphatic" (or "carbocycle" or "carbocyclyl") refers to a monocyclic C3-C8 hydrocarbon or bicyclic Cs-Cn hydrocarbon that is completely saturated or that contains one or more units of unsaturation, but which is not aromatic, that has a single point of attachment to the rest of the molecule wherein any individual ring in said bicyclic ring system has 3-7 members. Examples of cycloaliphatic groups include, but are not limited to, cycloalkyl and cycloalkenyl groups. Specific examples include, but are not limited to, cyclohexyl, cyclopropenyl, and cyclobutyl.
[00141] The term "heterocycle", "heterocyclyl", or "heterocyclic" as used herein means non-aromatic, monocyclic, bicyclic, or tricyclic ring systems in which one or more ring members are an independently selected heteroatom. In some embodiments, the "heterocycle", "heterocyclyl", or "heterocyclic" group has three to fourteen ring members in which one or more ring members is a heteroatom independently selected from oxygen, sulfur, nitrogen, or phosphorus, and each ring in the system contains 3 to 7 ring members. [00142] Examples of heterocycles include, but are not limited to, 3-l H-benzimidazol-2- one, 3-(l-alkyl)-benzimidazol-2-one, 2-tetrahydrofuranyl, 3-tetrahydrofuranyl, 2- tetrahydrothiophenyl, 3-tetrahydrothiophenyl, 2-morpholino, 3-morpholino, 4-morpholino, 2- thiomorpholino, 3-thiomorpholino, 4-thiomorpholino, 1-pyrrolidinyl, 2-pyrrolidinyl, 3- pyrrolidinyl, 1-tetrahydropiperazinyl, 2-tetrahydropiperazinyl, 3-tetrahydropiperazinyl, 1- piperidinyl, 2-piperidinyl, 3-piperidinyl, 1-pyrazolinyl, 3-pyrazolinyl, 4-pyrazolinyl, 5- pyrazolinyl, 1-piperidinyl, 2-piperidinyl, 3-piperidinyl, 4-piperidinyl, 2-thiazolidinyl, 3- thiazolidinyl, 4-thiazolidinyl, 1-imidazolidinyl, 2-imidazolidinyl, 4-imidazolidinyl, 5- imidazolidinyl, indolinyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, benzothiolane, benzodithiane, and 1 ,3-dihydro-imidazol-2-one.
[00143] Cyclic groups, (e.g. cycloaliphatic and heterocycles), can be linearly fused, bridged, or spirocyclic.
[00144] The term "heteroatom" means one or more of oxygen, sulfur, nitrogen, phosphorus, or silicon (including, any oxidized form of nitrogen, sulfur, phosphorus, or silicon; the quaternized form of any basic nitrogen or; a substitutable nitrogen of a heterocyclic ring, for example N (as in 3,4-dihydro-2H-pyrrolyl), NH (as in pyrrolidinyl) or NR+ (as in N-substituted pyrrolidinyl)).
[00145] The term "unsaturated", as used herein, means that a moiety has one or more units of unsaturation. As would be known by one of skill in the art, unsaturated groups can be partially unsaturated or fully unsaturated. Examples of partially unsaturated groups include, but are not limited to, butene, cyclohexene, and tetrahydropyridine. Fully unsaturated groups can be aromatic, anti-aromatic, or non-aromatic. Examples of fully unsaturated groups include, but are not limited to, phenyl, cyclooctatetraene, pyridyl, thienyl, and 1- methylpyridin-2(l H)-one.
[00146] The term "alkoxy", or "thioalkyl", as used herein, refers to an alkyl group, as previously defined, attached through an oxygen ("alkoxy") or sulfur ("thioalkyl") atom. [00147] The terms "haloalkyl", "haloalkenyl", "haloaliphatic", and "haloalkoxy" mean alkyl, alkenyl or alkoxy, as the case may be, substituted with one or more halogen atoms. This term includes perfluorinated alkyl groups, such as -CF3 and -CF2CF3. [00148] The terms "halogen", "halo", and "hal" mean F, Cl, Br, or I. [00149] The term "aryl" used alone or as part of a larger moiety as in "aralkyl", "aralkoxy", or "aryloxyalkyl", refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic and wherein each ring in the system contains 3 to 7 ring members. The term "aryl" may be used interchangeably with the term "aryl ring". [00150] The term "heteroaryl", used alone or as part of a larger moiety as in "heteroaralkyl" or "heteroarylalkoxy", refers to monocyclic, bicyclic, and tricyclic ring systems having a total of five to fourteen ring members, wherein at least one ring in the system is aromatic, at least one ring in the system contains one or more heteroatoms, and wherein each ring in the system contains 3 to 7 ring members. The term "heteroaryl" may be used interchangeably with the term "heteroaryl ring" or the term "heteroaromatic". Examples of heteroaryl rings include, but are not limited to, 2-furanyl, 3-furanyl, N- imidazolyl, 2-imidazolyl, 4-imidazolyl, 5-imidazolyl, benzimidazolyl, 3-isoxazolyl, 4- isoxazolyl, 5-isoxazolyl, 2-oxazolyl, 4-oxazolyl, 5-oxazolyl, N-pyrrolyl, 2-pyrrolyl, 3- pyrrolyl, 2-pyridyl, 3-pyridyl, 4-pyridyl, 2-pyrimidinyl, 4-pyrimidinyl, 5-pyrimidinyl, pyridazinyl (e.g., 3-pyridazinyl), 2-thiazolyl, 4-thiazolyl, 5-thiazolyl, tetrazolyl (e.g., 5- tetrazolyl), triazolyl (e.g., 2-triazolyl and 5-triazolyl), 2-thienyl, 3-thienyl, benzofuryl, benzothiophenyl, indolyl (e.g., 2-indolyl), pyrazolyl (e.g., 2-pyrazolyl), isothiazolyl, 1 ,2,3- oxadiazolyl, 1 ,2,5-oxadiazolyl, 1 ,2,4-oxadiazolyl, 1,2,3-triazolyl, 1,2,3-thiadiazolyl, 1 ,3,4- thiadiazolyl, 1,2,5-thiadiazolyl, purinyl, pyrazinyl, 1,3,5-triazinyl, quinolinyl (e.g., 2- quinolinyl, 3-quinolinyl, 4-quinolinyl), and isoquinolinyl (e.g., 1-isoquinolinyl, 3- isoquinolinyl, or 4-isoquinolinyl).
[00151] It shall be understood that the term "heteroaryl" includes certain types of heteroaryl rings that exist in equilibrium between two different forms. More specifically, for example, species such hydropyridine and pyridinone (and likewise hydroxypyrimidine and pyrimidinone) are meant to be encompassed within the definition of "heteroaryl."
Figure imgf000122_0001
[00152] The term "protecting group" and "protective group" as used herein, are interchangeable and refer to an agent used to temporarily block one or more desired functional groups in a compound with multiple reactive sites. In certain embodiments, a protecting group has one or more, or preferably all, of the following characteristics: a) is added selectively to a functional group in good yield to give a protected substrate that is b) stable to reactions occurring at one or more of the other reactive sites; and c) is selectively removable in good yield by reagents that do not attack the regenerated, deprotected ; functional group. As would be understood by one skilled in the art, in some cases, the reagents do not attack other reactive groups in the compound. In other cases, the reagents may also react with other reactive groups in the compound. Examples of protecting groups are detailed in Greene, T. W., Wuts, P. G in "Protective Groups in Organic Synthesis", Third Edition, John Wiley & Sons, New York: 1999 (and other editions of the book), the entire contents of which are hereby incorporated by reference. The term "nitrogen protecting group", as used herein, refers to an agent used to temporarily block one or more desired nitrogen reactive sites in a multifunctional compound. Preferred nitrogen protecting groups also possess the characteristics exemplified for a protecting group above, and certain exemplary nitrogen protecting groups are also detailed in Chapter 7 in Greene, T. W., Wuts, P. G in "Protective Groups in Organic Synthesis", Third Edition, John Wiley & Sons, New York: 1999, the entire contents of which are hereby incorporated by reference. [00153] In some embodiments, a methylene unit of an alkyl or aliphatic chain is optionally replaced with another atom or group. Examples of such atoms or groups include, but are not limited to, nitrogen, oxygen, sulfur, -C(O)-, -C(=N-CN>, -C(=NR)-, -C(=NOR)-, -SO-, and -SO2-. These atoms or groups can be combined to form larger groups. Examples of such larger groups include, but are not limited to, -OC(O)-, -C(O)CO-, -CO2-, -C(O)NR-, -C(=N- CN), -NRCO-, -NRC(O)O-, -SO2NR-, -NRSO2-, -NRC(O)NR-, -OC(O)NR-, and -NRSO2NR-, wherein R is, for example, H or C1-όaliphatic. It should be understood that these groups can be bonded to the methylene units of the aliphatic chain via single, double, or triple bonds. An example of an optional replacement (nitrogen atom in this case) that is bonded to the aliphatic chain via a double bond would be -CH2CH=N-CH3. In some cases, especially on the terminal end, an optional replacement can be bonded to the aliphatic group via a triple bond. One example of this would be CH2CH2CH2C=N. It should be understood that in this situation, the terminal nitrogen is not bonded to another atom. [00154] It should also be understood that, the term "methylene unit" can also refer to branched or substituted methylene units. For example, in an isopropyl moiety [-CH(CH3)2], a nitrogen atom (e.g. NR) replacing the first recited "methylene unit" would result in dimethylamine [-N(CH3)2]. In instances such as these, one of skill in the art would understand that the nitrogen atom will not have any additional atoms bonded to it, and the "R" from "NR" would be absent in this case.
[00155] Unless otherwise indicated, the optional replacements form a chemically stable compound. Optional replacements can occur both within the chain and/or at either end of the chain; i.e. both at the point of attachment and/or also at the terminal end. Two optional replacements can also be adjacent to each other within a chain so long as it results in a chemically stable compound. For example, a C3 aliphatic can be optionally replaced by 2 nitrogen atoms to form -C-N=N. The optional replacements can also completely replace all of the carbon atoms in a chain. For example, a C3 aliphatic can be optionally replaced by -NR-, -C(O)-, and -NR- to form -NRC(O)NR- (a urea).
[00156] Unless otherwise indicated, if the replacement occurs at the terminal end, the replacement atom is bound to a hydrogen atom on the terminal end. For example, if a methylene unit of -CH2CH2CH3 were optionally replaced with -O-, the resulting compound could be -OCH2CH3, -CH2OCH3, or -CH2CH2OH. It should be understood that if the terminal atom does not contain any free valence electrons, then a hydrogen atom is not required at the terminal end (e.g., -CH2CH2CH=O or -CH2CH2C≡N). [00157] Unless otherwise indicated, structures depicted herein are also meant to include all isomeric (e.g., enantiomeric, diastereomeric, geometric, conformational, and rotational) forms of the structure. For example, the R and S configurations for each asymmetric center, (Z) and (E) double bond isomers, and (Z) and (E) conformational isomers are included in this invention. As would be understood to one skilled in the art, a substituent can freely rotate
around any rotatable bonds. For example, a substituent drawn as
Figure imgf000124_0001
represents
Figure imgf000124_0002
[00158] Therefore, single stereochemical isomers as well as enantiomeric, diastereomeric, geometric, conformational, and rotational mixtures of the present compounds are within the scope of the invention.
[00159] Unless otherwise indicated, all tautomeric forms of the compounds of the invention are within the scope of the invention.
[00160] Additionally, unless otherwise indicated, structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the present structures except for the replacement of hydrogen by deuterium or tritium, or the replacement of a carbon by a 13C- or 14C-enriched carbon are within the scope of this invention. Such compounds are useful, for example, as analytical tools or probes in biological assays.
Pharmaceutically Acceptable Salts
[00161] The compounds of this invention can exist in free form for treatment, or where appropriate, as a pharmaceutically acceptable salt.
[00162] A "pharmaceutically acceptable salt" means any non-toxic salt of a compound of this invention that, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof. As used herein, the term "inhibitorily active metabolite or residue thereof means that a metabolite or residue thereof is also an inhibitor of the ATR protein kinase. [00163] Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge et al, describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 1977, 66, 1-19, incorporated herein by reference. Pharmaceutically acceptable salts of the compounds of this invention include those derived from suitable inorganic and organic acids and bases. These salts can be prepared in situ during the final isolation and purification of the compounds. Acid addition salts can be prepared by 1) reacting the purified compound in its free-based form with a suitable organic or inorganic acid and 2) isolating the salt thus formed.
[00164] Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, glycolate, gluconate, glycolate, hemisulfate, heptanoate, hexanoate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, palmoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, salicylate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. [00165] Base addition salts can be prepared by 1) reacting the purified compound in its acid form with a suitable organic or inorganic base and 2) isolating the salt thus formed. Salts derived from appropriate bases include alkali metal (e.g., sodium, lithium, and potassium), alkaline earth metal (e.g., magnesium and calcium), ammonium and N+(C1- 4alkyl)4 salts. This invention also envisions the quaternization of any basic nitrogen- containing groups of the compounds disclosed herein. Water or oil-soluble or dispersible products may be obtained by such quaternization.
[00166] Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, loweralkyl sulfonate and aryl sulfonate. Other acids and bases, while not in themselves pharmaceutically acceptable, may be employed in the preparation of salts useful as intermediates in obtaining the compounds of the invention and their pharmaceutically acceptable acid or base addition salts. Abbreviations
[00167] The following abbreviations are used:
DMSO dimethyl sulfoxide
ATP adenosine triphosphate
1HNMR proton nuclear magnetic resonance
HPLC high performance liquid chromatography
LCMS liquid chromatography-mass spectrometry
TLC thin layer chromatography
Rt retention time
Compound Uses
[00168] One aspect of this invention provides a compound for use in inhibiting ATR kinase. These compounds have formula I:
Figure imgf000126_0001
I
Or an acceptable salt thereof; wherein R1 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R1 is optionally substituted with 1-5 J1 groups;
R2 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R2 is optionally substituted with 1-5 J2 groups;
L is -C(O)NH- or -€(O)N(C1-6alkyl)-; n is 0 or 1 ;
Each J1 and J2 is independently halo, -CN, -NO2, -V'-R, or -(V2)m-Q;
V1 is a C1-10aliphatic chain wherein 0-3 methylene units are optionally and independently replaced with O, NR", S, C(O), S(O), or S(O)2; V1 is optionally substituted with 1-6 occurrences of Jvι;
V2 is a C1-10aliphatic chain wherein 0-3 methylene units are optionally and independently replaced with O, NR", S, C(O), S(O), or S(O)2; V2 is optionally substituted with 1-6 occurrences or J ; m is O or 1 ;
Q is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 9-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q is optionally substituted with 0-5 JQ; each JV1 or JV2 is independently halogen, CN, NH2, NO2, C1-4aliphatic, NH(C1 -4aliphatic), N(C1 -4aliphatic)2, OH, O(C1-4aliphatic), CO2H, CO2(C1-4aliphatic), C(O)NH2, C(O)NH(C i .4aliphatic), C(O)N(C , -4al iphatic)2, NHCO(C i .4aliphatic), N(C1-4aliphatic)CO(C1-4aliphatic), SO2(C1-4aliphatic), NHSO2(C1-4aliphatic), or N(C1-4aliphatic)SO2(C1-4aliphatic), wherein said C1-4aliphatic is optionally substituted with halo;
R is H or C1-6aliphatic wherein said C1-6aliphatic is optionally substituted with 1-4 occurrences OfNH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO2(C1-4aliphatic), CO(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; each JQ is independently halo, oxo, CN, NO2, X-R, or -(X)P-Q4; p is O or 1 ;
X is C1-10aliphatic; wherein 1-3 methylene units of said C1-6aliphatic are optionally replaced with -NR, -0-, -S-, C(O), S(O)2, or S(O); wherein X is optionally and independently substituted with 1-4 occurrences OfNH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO(C1-4aliphatic), CO2H, CO2(C1-4aliphatic), C(O)NH2, C(O)NH(C1-4aliphatic), C(O)N(C1-4aliphatic)2, SO(C1 -4aliphatic), SO2(C1-4aliphatic), SO2NH(C i.4aliphatic), SO2N(C1-4aliphatic)2, NHC(O)(C1-4aliphatic), N(C1-4aliphatic)C(O)(C1-4aliphatic), wherein said C1-4aliphatic is optionally substituted with 1-3 occurrences of halo;
Q4 is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 8-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q4 is optionally substituted with 1-5 JQ4;
JQ4 is halo, CN, or C1-4alkyl wherein up to 2 methylene units are optionally replaced with O, NR*, S, C(O), S(O), or S(O)2;
R is H or C1-4alkyl wherein said C1-4alkyl is optionally substituted with 1-4 halo;
R', R", and R* are each independently H, C1-4alkyl, or is absent; wherein said C1-4alkyl is optionally substituted with 1-4 halo.
[00169] In one embodiment, R1 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R1 is optionally substituted with 1-5 J1 groups;
R2 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R2 is optionally substituted with 1-5 J2 groups; each JV1 or JV2 is independently NH2, NH(C1-4a!iphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO2(C1-4aliphatic), C0(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; each JQ is independently halogen, NO2, CN, or C1-6aliphatic wherein up to 1 methylene unit is optionally replaced with NR', O, S, CO, CO2, CONR', SO, SO2, SO2NR', OCO, NR'CO, NR'COO, NR'SO, NR5SO2, NR1SO2NR', OCONR', or NR'CONR'; wherein said C1-6aliphatic is optionally substituted with 1-4 substituents selected from NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO2 (C1-4aliphatic), CO(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic.
[00170] In some embodiments, R2 is substituted with one or two occurrences of J2. In . oher embodiments,
[00171] In some embodiments, R2 is a 5-6 membered monocyclic aromatic ring. In some embodiments, R2 is a 6-membered aromatic ring. In other embodiments, R2 is phenyl or pyridyl. In yet other embodiments, R2 is phenyl.
[00172] R2 is optionally substituted with 1-5 J2 groups. In some embodiments, 1-3 J2 groups, and in other embodiments, 1-2 J2 groups. In yet another embodiment, R2 is substituted with O or 1 occurrences of J2. In some embodiments, J2 is -(V2)m-Q or V'-R; wherein each V1 and V2 is independently a C1-6aliphatic chain wherein 0-3 methylene units are optionally replaced with O, NR', S, C(O), S(O), or S(O)2; wherein the first or second methylene group away from the point of attachment is replaced with C(O), S(O), or S(O)2, S or O; m is 1; R is H; and
Q is a 5-7 membered monocyclic ring containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said Q is optionally substituted with 1-3 occurrences of halogen, C1-3alkyl, CN, OH, O(C1-3alkyl), NH2, NH(d.3alkyl), N(C1-3alkyl)2, or CO(C1-3alkyl).
[00173] In some embodiments, Q is a 5-6 membered monocyclic ring.
[00174] Another to another embodiment, n is O. [00175] In some embodiments, n is 0 and R1 is a 5-6 membered monocyclic aromatic ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 9-10 membered bicyclic aromatic ring having 1-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; wherein each R1 is optionally substituted with 1-5 J1 groups. [00176] In some embodiments, R1 is benzothiazole, oxadiazole, benzoxazole, triazole, thiadiazole, or isoxazole. In other embodiments, R1 is benzimidazole, benzothioazole, oxadiazole, isoxazole, or triazole. In some embodiments, R1 is benzimidazole. In other embodiments, R1 is isoxazole.
[00177] R1 is optionally substituted with 1-5 J1 groups. In some embodiments, 1-3 J1 groups, and in other embodiments, 1-2 J1 groups. In some embodiments, J1 is halo, CN, NO2, or -V'-R. In other embodiments, J1 is halo, CN, C1-6alkyl, OR, SR, NR"R, C(O)R, C(O)OR, C(O)NR"R, S(O)2R, or S(O)R. In yet other embodiments, J1 is phenyl optionally substituted with NH2, C1-4alkyl, thiophene, or CH2NH2.
[00178] In some embodiments, R2 is a 5-6 membered monocyclic aromatic ring. In some embodiments, R2 is a 6-membered aromatic ring. In other embodiments, R2 is phenyl or pyridyl. In yet other embodiments, R2 is phenyl.
[00179] In another embodiment, J2 is -V'-R or -<V2)m-Q; wherein V1 and V2 are O, NR", -CO-, or -SO2-; Q is a 5-6 membered heterocyclic ring containing 1-2 heteroatoms selected from N or O; and JV1 and JV2 are halo. In another embodiment, J2 is SO2CH3, morpholinyl, CH2OH, C(O)-(morpholinyl), C(O)NH(C1-4alkyl)OH, piperazinyl, CN, CH2NHC(O)CH3, halo, C(O)NH(C1-4alkyl)pyrrolidinyl, or SO2(pyrrolidinyl). [00180] In some embodiments, R2 is phenyl or pyridyl. [00181] In some embodiments, J2 is SO2(C1-4alkyl). [00182] In one embodiment, n is 0;
R1 is benzimidazole, benzothiazole, benzoxazole, oxadiazole, isoxazole, thiadiazole, or triazole;
J1 is halo, CN, NO2, or -V'-R;
R2 is phenyl or pyridyl;
Figure imgf000130_0001
m is 1 ; V1 and V2 are -SO2- -O-, -NR-, or -CO -;
Q is a 5-6 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen or oxygen; and
R is H or C1-6alkyl wherein said C1-6alkyl is optionally substituted with 1-4 halo. [00183] Acording to another embodiment, n is 1.
[00184] In some embodiments, R1 is phenyl, pyridyl, pyrimidyl, pyrazinyl, piperonyl, indolyl, benzimidazolyl, indazolyl, benzothiazolyl, benzothiophenyl (i.e. benzothienyl), benzoxazolyl, pyrrolopyrimidinyl, pyrrolopyridinyl, azaindazolyl, or azaindolyl. In other embodiments, R1 is phenyl, pyridyl, pyrimidyl, piperonyl, or indole. In yet other embodiments, R1 is phenyl.
[00185] R1 is optionally substituted with 1-5 J1 groups. In some embodiments, 1-3 J1 groups, and in other embodiments, 1-2 J1 groups. In some embodiments, J1 is C1-6alkyl, CN, halo, OR, NR"R, SR, COR, CO2R, CONR"R, SOR, SO2R, S(O)2NR"R, OCOR, NRC(O)R, NRCOOR, NRSOR, NRSO2R, NRS02NR"R, OCONR"R, or NRCONR"R; 4-6 membered fully saturated monocyclic ring containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur. In other embodiments, J1 is C1-6alkyl, CN, halo, OR, NR"R, CONR"R, S(O)2NR51R, NC(O)R, or pyrrolidinyl.
[00186] In some embodiments, R2 is phenyl, pyridyl, pyrimidyl, indole, furanyl, pyrazole, thiophene, tetrahydropyran, or indazole. R2 is optionally substituted with 1-5 J2 groups. In some embodiments, 1-3 J2 groups, and in other embodiments, 1-2 J2 groups. In some embodiments, J2 is halo, CN, NR"R, C1-6alkyl, OR, SO2R, NHSO2R, COOR, CONR"R, morpholinyl, -V'-R, or -(V2)m-Q, wherein
V1 and V2 are CO, -CONR"-, -CONR"-(C1-4alkyl)-,
-CONR"-(C1-4alkyl)-OCH2-, -CONR"-(C1-4alkyl)-N(CH3)-, R is H or C1-4alkyl; and
Q is 1,4-diazepanyl, azetidinyl optionally substituted with OMe, piperidinyl optionally substituted with C1-4alkyl, 4-CH2OH, CONH2, pyrrolidinyl, OH, or CH2-pyrrolidinyl; piperazinyl optionally substituted with CH2CH2CN, CH3, COCH3, pyrrolidinyl optionally substituted with dimethylamino, tetrahydropyran, C3-iocyclolkyl optionally substituted with OH. [00187] In other embodiments, J2 is SO2CH3, NHSO2CH3, CN, OH, OCH3, F, N(CH3)2, NHSO2CH3, CF3, C1-6alkyl, CO(l ,4-diazepanyl), COOH, CONH2, CON(CH3)2) CO(azetidinyl), CON(CH3)(C1-4alky I)OCH3, CONH(C1-4alkyl)piperazinyl, CONH(C1-4alkyl)piperidinyl, CONH-tetrahydropyran, CON(methylpiperidinyl), CO(piperidinyl), CONH-cyclopropyl, CO(morpholinyl), CON(CH3)-(C1-4alkyl)-N(CH3)2, CO(piperazinyl), CONH-(C1-4alkyl)-pyrrolidinyl, CONH-(C1-4alkyl)-piperidinyl, CONH-(C1-4alkyl)-tetrahydropyranyl, morpholinyl, CO(pyrrolidinyl), CO(piperidinyl), CO(pyrrolidinyl), CH2-pyrrolidinyl, or CONH(cyclohexyl), wherein said J2 is optionally substituted with C1-4alkyl, CONH2, pyrrolidinyl, OH, O(C1-4alkyl), NH2, NH(C1-4alkyl), N(C1-4alkyl)2, -(C1-4alky I)-CN, -(C1-4alkyl)-OH, -(C1-4alkyl)-N(C1-4alkyl)2,or CO(C1-4alkyl). [00188] In some embodiments, n is 1 ;
R1 is phenyl; R2 is phenyl, pyridyl, indole, furanyl, pyrazole, thiophene, tetrahydropyran, or indazole; J2 is halo, CN, NR"R, C1-6alkyl, OR, SO2R, NHSO2R, COOR, CONR"R, morpholinyl, -V'-R, or -(V2)m-Q, wherein m is 1 ; V1 and V2 are CO, -CONR-, -CONR-(C1-4alkyl)-, -CONR-(C1-4alky I)-OCH2-, or -CONR-(C1-4alky I)-N(CH3)-; R is H or C1-4alkyl; and
Q is 1 ,4-diazepanyl, azetidinyl optionally substituted with OMe, piperidinyl optionally substituted with C1-4alkyl, 4-CH2OH, CONH2, pyrrolidinyl, OH, or CH2-pyrrolidinyl; piperazinyl optionally substituted with CH2CH2CN, CH3, COCH3, pyrrolidinyl optionally substituted with dimethylamino, tetrahydropyran, C3-10cyclolkyl optionally substituted with OH; and J1 is C1-6alkyl, CN, halo, OR, NR"R, SR, COR, CO2R, CO NR"R, SOR,
SO2R, S(O)2NR"R, OCOR, NRC(O)R, NRCOOR, NRSOR, NRSO2R, NRSO2NR11R, 0C0NR"R, orNRCONR"R; 4-6 membered fully saturated monocyclic ring containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur. [00189] In other embodiments, n is O;
R1 is benzimidazole, benzothioazole, oxadiazole, isoxazole, or triazole;
J1 is halo, CN, NO2, or -V'-R;
R2 is phenyl or pyridyl;
J2 is — (V2)m-Q or -V'-R; m is 1 ;
V1 and V2 are -SO2-, -O-, -NR"-, or -CO -;
Q is a 5-6 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen or oxygen; and
R is H or C1-6alkyl wherein said C1-6alkyl is optionally substituted with 1-4 halo.
[00190] In yet other embodiments, the compound is selected from Table 1 (above). [00191] One aspect of this invention provides compounds that are inhibitors of ATR kinase, and thus are useful for treating or lessening the severity of a disease, condition, or disorder where ATR is implicated in the disease, condition, or disorder. [00192] Another aspect of this invention provides compounds that are useful for the treatment of diseases, disorders, and conditions characterized by excessive or abnormal cell proliferation. Such diseases include, a proliferative or hyperproliferative disease. Examples of proliferative and hyperproliferative diseases include, without limitation, cancer and myeloproliferative disorders.
[00193] In some embodiments, said compounds are selected from the group consisting of a compound of formula I, II, III, IV, IA, HA, IHA, IVA, IA-i, IA-ii, IA-iii, V, VI, and VII. [00194] The term "cancer" includes, but is not limited to the following cancers. Oral: buccal cavity, lip, tongue, mouth, pharynx; Cardiac: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma; Lung: bronchogenic carcinoma (squamous cell or epidermoid, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma; Gastrointestinal: esophagus (squamous cell carcinoma, larynx, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leiomyosarcoma), pancreas (ductal adenocarcinoma, insulinoma, glucagonoma, gastrinoma, carcinoid tumors, vipoma), small bowel or small intestines (adenocarcinoma, lymphoma, carcinoid tumors, Karposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma, fibroma), large bowel or large intestines (adenocarcinoma, tubular adenoma, villous adenoma, hamartoma, leiomyoma), colon, colon- rectum, colorectal; rectum, Genitourinary tract: kidney (adenocarcinoma, Wilm's tumor [nephroblastoma], lymphoma, leukemia), bladder and urethra (squamous cell carcinoma, transitional cell carcinoma, adenocarcinoma), prostate (adenocarcinoma, sarcoma), testis (seminoma, teratoma, embryonal carcinoma, teratocarcinoma, choriocarcinoma, sarcoma, interstitial cell carcinoma, fibroma, fibroadenoma, adenomatoid tumors, lipoma); Liver: hepatoma (hepatocellular carcinoma), cholangiocarcinoma, hepatoblastoma, angiosarcoma, hepatocellular adenoma, hemangioma, biliary passages; Bone: osteogenic sarcoma (osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma, chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cell sarcoma), multiple myeloma, malignant giant cell tumor chordoma, osteochronfroma (osteocartilaginous exostoses), benign chondroma, chondroblastoma, chondromyxofibroma, osteoid osteoma and giant cell tumors; Nervous system: skull (osteoma, hemangioma, granuloma, xanthoma, osteitis deformans), meninges (meningioma, meningiosarcoma, gliomatosis), brain (astrocytoma, medulloblastoma, glioma, ependymoma, germinoma [pinealoma], glioblastoma multiform, oligodendroglioma, schwannoma, retinoblastoma, congenital tumors), spinal cord neurofibroma, meningioma, glioma, sarcoma); Gynecological: uterus (endometrial carcinoma), cervix (cervical carcinoma, pre-tumor cervical dysplasia), ovaries (ovarian carcinoma [serous cystadenocarcinoma, mucinous cystadenocarcinoma, unclassified carcinoma], granulosa- thecal cell tumors, Sertoli-Leydig cell tumors, dysgerminoma, malignant teratoma), vulva (squamous cell carcinoma, intraepithelial carcinoma, adenocarcinoma, fibrosarcoma, melanoma), vagina (clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma (embryonal rhabdomyosarcoma), fallopian tubes (carcinoma), breast; Hematologic: blood (myeloid leukemia [acute and chronic], acute lymphoblastic leukemia, chronic lymphocytic leukemia, myeloproliferative diseases, multiple myeloma, myelodysplastic syndrome), Hodgkin's disease, non-Hodgkin's lymphoma [malignant lymphoma] hairy cell; lymphoid disorders; Skin: malignant melanoma, basal cell carcinoma, squamous cell carcinoma, Karposi's sarcoma, keratoacanthoma, moles dysplastic nevi, lipoma, angioma, dermatofibroma, keloids, psoriasis, Thyroid gland: papillary thyroid carcinoma, follicular thyroid carcinoma; [note to scientist: do we want to added "undifferentiated thyroid cancer"? - from original] medullary thyroid carcinoma, multiple endocrine neoplasia type 2A, multiple endocrine neoplasia type 2B, familial medullary thyroid cancer, pheochromocytoma, paraganglioma; and Adrenal glands: neuroblastoma. [00195] Thus, the term "cancerous cell" as provided herein, includes a cell afflicted by any one of the above-identified conditions. In some embodiments, the cancer is selected from colorectal, thyroid, or breast cancer.
[00196] The term "myeloproliferative disorders", includes disorders such as polycythemia vera, thrombocythemia, myeloid metaplasia with myelofibrosis, hypereosinophilic syndrome, juvenile myelomonocytic leukemia, systemic mast cell disease, and hematopoietic disorders, in particular, acute-myelogenous leukemia (AML), chronic-myelogenous leukemia (CML), acute-promyelocytic leukemia (APL), and acute lymphocytic leukemia (ALL).
Pharmaceutically Acceptable Derivatives or Prodrugs
[00197] In addition to the compounds of this invention, pharmaceutically acceptable derivatives or prodrugs of the compounds of this invention may also be employed in compositions to treat or prevent the herein identified disorders.
[00198] The compounds of this invention can also exist as pharmaceutically acceptable derivatives.
[00199] A "pharmaceutically acceptable derivative" is an adduct or derivative which, upon administration to a patient in need, is capable of providing, directly or indirectly, a compound as otherwise described herein, or a metabolite or residue thereof. Examples of pharmaceutically acceptable derivatives include, but are not limited to, esters and salts of such esters.
[00200] A "pharmaceutically acceptable derivative or prodrug" means any pharmaceutically acceptable ester, salt of an ester or other derivative or salt thereof of a compound, of this invention which, upon administration to a recipient, is capable of providing, either directly or indirectly, a compound of this invention or an inhibitorily active metabolite or residue thereof. Particularly favoured derivatives or prodrugs are those that increase the bioavailability of the compounds of this invention when such compounds are administered to a patient (e.g., by allowing an orally administered compound to be more readily absorbed into the blood) or which enhance delivery of the parent compound to a biological compartment (e.g., the brain or lymphatic system) relative to the parent species. [00201] Pharmaceutically acceptable prodrugs of the compounds of this invention include, without limitation, esters, amino acid esters, phosphate esters, metal salts and sulfonate esters.
Pharmaceutical Compositions
[00202] The present invention also provides compounds and compositions that are useful as inhibitors of ATR kinase.
[00203] One aspect of this invention provides pharmaceutically acceptable compositions that comprise any of the compounds as described herein, and optionally comprise a pharmaceutically acceptable carrier, adjuvant or vehicle.
[00204] The pharmaceutically acceptable carrier, adjuvant, or vehicle, as used herein, includes any and all solvents, diluents, or other liquid vehicle, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired. Remington's
Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa.,
1980) discloses various carriers used in formulating pharmaceutically acceptable compositions and known techniques for the preparation thereof. Except insofar as any conventional carrier medium is incompatible with the compounds of the invention, such as by producing any undesirable biological effect or otherwise interacting in a deleterious manner with any other component(s) of the pharmaceutically acceptable composition, its use is contemplated to be within the scope of this invention.
[00205] Some examples of materials which can serve as pharmaceutically acceptable carriers include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, or potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, polyacrylates, waxes, polyethylene- polyoxypropylene-block polymers, wool fat, sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil; safflower oil; sesame oil; olive oil; corn oil and soybean oil; glycols; such a propylene glycol or polyethylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol, and phosphate buffer solutions, as well as other non-toxic compatible lubricants such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, releasing agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the composition, according to the judgment of the formulator.
Combination Therapies
[00206] Another aspect of this invention is directed towards a method of treating cancer in a subject in need thereof, comprising administration of a compound of this invention or a pharmaceutically acceptable salt thereof, and an additional therapeutic agent. In some embodiments, said method comprises the sequential or co-administration of the compound or a pharmaceutically acceptable salt thereof, and the additional therapeutic agent.
[00207] In some embodiments, said additional therapeutic agent is an anti-cancer agent.
In other embodiments, said additional therapeutic agent is a DNA-damaging agent. In yet other embodiments, said additional therapeutic agent is selected from radiation therapy, chemotherapy, or other agents typically used in combination with radiation therapy or chemotherapy, such as radiosensitizers and chemosensitizers.
[00208] As would be known by one of skill in the art, radiosensitizers are agents that can be used in combination with radiation therapy. Radiosensitizers work in various different ways, including, but not limited to, making cancer cells more sensitive to radiation therapy, working in synergy with radiation therapy to provide an improved synergistic effect, acting additively with radiation therapy, or protecting surrounding healthy cells from damage caused by radiation therapy. Likewise chemosensitizers are agents that can be used in combination with chemotherapy. Similarly, chemosensitizers work in various different ways, including, but not limited to, making cancer cells more sensitive to chemotherapy, working in synergy with chemotherapy to provide an improved synergistic effect, acting additively to chemotherapy, or protecting surrounding healthy cells from damage caused by chemotherapy.
[00209] Examples of DNA-damaging agents that may be used in combination with compounds of this invention include, but are not limited to Platinating agents, such as Carboplatin, Nedaplatin, Satraplatin and other derivatives; Topo I inhibitors, such as Topotecan, irinotecan/SN38, rubitecan and other derivatives; Antimetabolites, such as Folic family (Methotrexate, Pemetrexed and relatives); Purine antagonists and Pyrimidine antagonists (Thioguanine, Fludarabine, Cladribine, Cytarabine, Gemcitabine, 6-Mercaptopurine, 5-Fluorouracil (5FU) and relatives); Alkylating agents, such as Nitrogen mustards (Cyclophosphamide, Melphalan, Chlorambucil, mechlorethamine, Ifosfamide and relatives); nitrosoureas (eg Carmustine); Triazenes (Dacarbazine, temozolomide); Alkyl sulphonates (eg Busulfan); Procarbazine and Aziridines; Antibiotics, such as Hydroxyurea, Anthracyclines (doxorubicin, daunorubicin, epirubicin and other derivatives); Anthracenediones (Mitoxantrone and relatives); Streptomyces family (Bleomycin, Mitomycin C, actinomycin); and Ultraviolet light.
[00210] Other therapies or anticancer agents that may be used in combination with the inventive agents of the present invention include surgery, radiotherapy (in but a few examples, gamma-radiation, neutron beam radiotherapy, electron beam radiotherapy, proton therapy, brachy therapy, and systemic radioactive isotopes, to name a few), endocrine therapy, biologic response modifiers (interferons, interleukins, and tumor necrosis factor (TNF) to name a few), hyperthermia and cryotherapy, agents to attenuate any adverse effects (e.g., antiemetics), and other approved chemotherapeutic drugs, including, but not limited to, the DNA damaging agents listed herein, spindle poisons (Vinblastine, Vincristine, Vinorelbine, Paclitaxel), podophyllotoxins (Etoposide, Irinotecan, Topotecan), nitrosoureas (Carmustine, Lomustine), inorganic ions (Cisplatin, Carboplatin), enzymes (Asparaginase), and hormones (Tamoxifen, Leuprolide, Flutamide, and Megestrol), Gleevec™, adriamycin, dexamethasone, and cyclophosphamide.
[00211] A compound of the instant invention may also be useful for treating cancer in combination with any of the following therapeutic agents: abarelix (Plenaxis depot®); aldesleukin (Prokine®); Aldesleukin (Proleukin®); Alemtuzumabb (Campath®); alitretinoin (Panretin®); allopurinol (Zyloprim®); altretamine (Hexalen®); amifostine (Ethyol®); anastrozole (Arimidex®); arsenic trioxide (Trisenox®); asparaginase (Elspar®); azacitidine (Vidaza®); bevacuzimab (Avastin®); bexarotene capsules (Targretin®); bexarotene gel (Targretin®); bleomycin (Blenoxane®); bortezomib (Velcade®); busulfan intravenous (Busulfex®); busulfan oral (Myleran®); calusterone (Methosarb®); capecitabine (Xeloda®); carboplatin (Paraplatin®); carmustine (BCNU®, BiCNU®); carmustine (Gliadel®); carmustine with Polifeprosan 20 Implant (Gliadel Wafer®); celecoxib (Celebrex®); cetuximab (Erbitux®); chlorambucil (Leukeran®); cisplatin (Platinol®); cladribine (Leustatin®, 2-CdA®); clofarabine (Clolar®); cyclophosphamide (Cytoxan®, Neosar®); cyclophosphamide (Cytoxan Injection®); cyclophosphamide (Cytoxan Tablet®); cytarabine (Cytosar-U®); cytarabine liposomal (DepoCyt®); dacarbazine (DTIC-Dome®); dactinomycin, actinomycin D (Cosmegen®); Darbepoetin alfa (Aranesp®); daunorubicin liposomal (DanuoXome®); daunorubicin, daunomycin (Daunorubicin®); daunorubicin, daunomycin (Cerubidine®); Denileukin diftitox (Ontak®); dexrazoxane (Zinecard®); docetaxel (Taxotere®); doxorubicin (Adriamycin PFS®); doxorubicin (Adriamycin®, Rubex®); doxorubicin (Adriamycin PFS Injection®); doxorubicin liposomal (Doxil®); dromostanolone propionate (dromostanolone®); dromostanolone propionate (masterone injection®); Elliott's B Solution (Elliott's B Solution®); epirubicin (Ellence®); Epoetin alfa (epogen®); erlotinib (Tarceva®); estramustine (Emcyt®); etoposide phosphate (Etopophos®); etoposide, VP- 16 (Vepesid®); exemestane (Aromasin®); Filgrastim (Neupogen®); floxuridine (intraarterial) (FUDR®); fludarabine (Fludara®); fluorouracil, 5- FU (Adrucil®); fulvestrant (Faslodex®); gefitinib (Iressa®); gemcitabine (Gemzar®); gemtuzumab ozogamicin (Mylotarg®); goserelin acetate (Zoladex Implant®); goserelin acetate (Zoladex®); histrelin acetate (Histrelin implant®); hydroxyurea (Hydrea®); Ibritumomab Tiuxetan (Zevalin®); idarubicin (Idamycin®); ifosfamide (IFEX®); imatinib mesylate (Gleevec®); interferon alfa 2a (Roferon A®); Interferon alfa-2b (Intron A®); irinotecan (Camptosar®); lenalidomide (Revlimid®); letrozole (Femara®); leucovorin (Wellcovorin®, Leucovorin®); Leuprolide Acetate (Eligard®); levamisole (Ergamisol®); lomustine, CCNU (CeeBU®); meclorethamine, nitrogen mustard (Mustargen®); megestrol acetate (Megace®); melphalan, L-PAM (Alkeran®); mercaptopurine, 6-MP (Purinethol®); mesna (Mesnex®); mesna (Mesnex tabs®); methotrexate (Methotrexate®); methoxsalen (Uvadex®); mitomycin C (Mutamycin®); mitotane (Lysodren®); mitoxantrone (Novantrone®); nandrolone phenpropionate (Durabolin-50®); nelarabine (Arranon®); Nofetumomab (Verluma®); Oprelvekin (Neumega®); oxaliplatin (Eloxatin®); paclitaxel (Paxene®); paclitaxel (Taxol®); paclitaxel protein-bound particles (Abraxane®); palifermin (Kepivance®); pamidronate (Aredia®); pegademase (Adagen (Pegademase Bovine)®); pegaspargase (Oncaspar®); Pegfilgrastim (Neulasta®); pemetrexed disodium (Alimta®); pentostatin (Nipent®); pipobroman (Vercyte®); plicamycin, mithramycin (Mithracin®); porfimer sodium (Photofrin®); procarbazine (Matulane®); quinacrine (Atabrine®); Rasburicase (Elitek®); Rituximab (Rituxan®); sargramostim (Leukine®); Sargramostim (Prokine®); sorafenib (Nexavar®); streptozocin (Zanosar®); sunitinib maleate (Sutent®); talc (Sclerosol®); tamoxifen (Nolvadex®); temozolomide (Temodar®); teniposide, VM-26 (Vumon®); testolactone (Teslac®); thioguanine, 6-TG (Thioguanine®); thiotepa (Thioplex®); topotecan (Hycamtin®); toremifene (Fareston®); Tositumomab (Bexxar®); Tositumomab/I-131 tositumomab (Bexxar®); Trastuzumab (Herceptin®); tretinoin, ATRA (Vesanoid®); Uracil Mustard (Uracil Mustard Capsules®); valrubicin (Valstar®); vinblastine (Velban®); vincristine (Oncovin®); vinorelbine (Navelbine®); zoledronate (Zometa®) and vorinostat (Zolinza®).
[00212] For a comprehensive discussion of updated cancer therapies see, http://www.nci.nih.gov/, a list of the FDA approved oncology drugs at http://www.fda.gov/cder/cancer/druglistframe.htm, and The Merck Manual, Seventeenth Ed. 1999, the entire contents of which are hereby incorporated by reference. Compositions for Administration into a Subject
[00213] The ATR kinase inhibitors or pharmaceutical salts thereof may be formulated into pharmaceutical compositions for administration to animals or humans. These pharmaceutical compositions, which comprise an amount of the ATR inhibitor effective to treat or prevent the diseases or conditions described herein and a pharmaceutically acceptable carrier, are another embodiment of the present invention. [00214] The exact amount of compound required for treatment will vary from subject to subject, depending on the species, age, and general condition of the subject, the severity of the infection, the particular agent, its mode of administration, and the like. The compounds of the invention are preferably formulated in dosage unit form for ease of administration and uniformity of dosage. The expression "dosage unit form" as used herein refers to a physically discrete unit of agent appropriate for the patient to be treated. It will be understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific effective dose level for any particular patient or organism will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidental with the specific compound employed, and like factors well known in the medical arts. The term "patient", as used herein, means an animal, preferably a mammal, and most preferably a human.
[00215] In some embodiments, these compositions optionally further comprise one or more additional therapeutic agents. For example, chemotherapeutic agents or other antiproliferative agents may be combined with the compounds of this invention to treat proliferative diseases and cancer. Examples of known agents with which these compositions can be combined are listed above under the "Combination Therapies" section and also throughout the specification. Some embodiments provide a simultaneous, separate or sequential use of a combined preparation. Modes of Administration and Dosage Forms
[00216] The pharmaceutically acceptable compositions of this invention can be administered to humans and other animals orally, rectally, parenterally, intracisternally, intravaginally, intraperitoneally, topically (as by powders, ointments, or drops), bucally, as an oral or nasal spray, or the like, depending on the severity of the infection being treated. In certain embodiments, the compounds of the invention may be administered orally or parenterally at dosage levels of about 0.01 mg/kg to about 50 mg/kg and preferably from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.
[00217] Liquid dosage forms for oral administration include, but are not limited to, pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
[00218] Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U. S. P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables. [00219] The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.
[00220] In order to prolong the effect of a compound of the present invention, it is often desirable to slow the absorption of the compound from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the compound then depends upon its rate of dissolution that, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenteral Iy administered compound form is accomplished by dissolving or suspending the compound in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the compound in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of compound to polymer and the nature of the particular polymer employed, the rate of compound release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the compound in liposomes or microemulsions that are compatible with body tissues.
[00221] Compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.
[00222] Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar—agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may also comprise buffering agents. [00223] Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes. Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polethylene glycols and the like.
[00224] The active compounds can also be in microencapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active compound may be admixed with at least one inert diluent such as sucrose, lactose or starch. Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and microcrystalline cellulose. In the case of capsules, tablets and pills, the dosage forms may also comprise buffering agents. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes.
[00225] Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches. The active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required. Ophthalmic formulation, eardrops, and eye drops are also contemplated as being within the scope of this invention. Additionally, the present invention contemplates the use of transdermal patches, which have the added advantage of providing controlled delivery of a compound to the body. Such dosage forms can be made by dissolving or dispensing the compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel. [00226] The compositions of the present invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir. The term "parenteral" as used herein includes, but is not limited to, subcutaneous, intravenous, intramuscular, intra-articular, intra-synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques. Preferably, the compositions are administered orally, intraperitoneally or intravenously. [00227] Sterile injectable forms of the compositions of this invention may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a nontoxic parenteral ly-acceptable diluent or solvent, for example as a solution in 1 ,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil may be employed including synthetic mono- or di-glycerides. Fatty acids, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutical ly-acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions. These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents which are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions. Other commonly used surfactants, such as Tweens, Spans and other emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms may also be used for the purposes of formulation.
[00228] The pharmaceutical compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, aqueous suspensions or solutions. In the case of tablets for oral use, carriers commonly used include, but are not limited to, lactose and corn starch. Lubricating agents, such as magnesium stearate, are also typically added. For oral administration in a capsule form, useful diluents include lactose and dried cornstarch. When aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents may also be added. [00229] Alternatively, the pharmaceutical compositions of this invention may be administered in the form of suppositories for rectal administration. These can be prepared by mixing the agent with a suitable non-irritating excipient that is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug. Such materials include, but are not limited to, cocoa butter, beeswax and polyethylene glycols. [00230] The pharmaceutical compositions of this invention may also be administered topically, especially when the target of treatment includes areas or organs readily accessible by topical application, including diseases of the eye, the skin, or the lower intestinal tract. Suitable topical formulations are readily prepared for each of these areas or organs. [00231] Topical application for the lower intestinal tract can be effected in a rectal suppository formulation (see above) or in a suitable enema formulation. Topically- transdermal patches may also be used.
[00232] For topical applications, the pharmaceutical compositions may be formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers. Carriers for topical administration of the compounds of this invention include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water. Alternatively, the pharmaceutical compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers. Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.
[00233] For ophthalmic use, the pharmaceutical compositions may be formulated as micronized suspensions in isotonic, pH adjusted sterile saline, or, preferably, as solutions in isotonic, pH adjusted sterile saline, either with or without a preservative such as benzylalkonium chloride. Alternatively, for ophthalmic uses, the pharmaceutical compositions may be formulated in an ointment such as petrolatum. [00234] The pharmaceutical compositions of this invention may also be administered by nasal aerosol or inhalation. Such compositions are prepared according to techniques well- known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents. [00235] The amount of protein kinase inhibitor that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated, the particular mode of administration. Preferably, the compositions should be formulated so that a dosage of between 0.01 - 100 mg/kg body weight/day of the inhibitor can be administered to a patient receiving these compositions.
[00236] It should also be understood that a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated. The amount of inhibitor will also depend upon the particular compound in the composition.
Administering with another Agent
[00237] Depending upon the particular protein kinase-mediated conditions to be treated or prevented, additional drugs, which are normally administered to treat or prevent that condition, may be administered together with the compounds of this invention.
[00238] Those additional agents may be administered separately, as part of a multiple dosage regimen, from the protein kinase inhibitor-containing compound or composition. Alternatively, those agents may be part of a single dosage form, mixed together with the protein kinase inhibitor in a single composition.
[00239] Another aspect of this invention is directed towards a method of treating cancer in a subject in need thereof, comprising the sequential or co-administration of a compound of this invention or a pharmaceutically acceptable salt thereof, and an anti-cancer agent. In some embodiments, said anti-cancer agent is selected from Platinating agents, such as Cisplatin, Oxaliplatin, Carboplatin, Nedaplatin, or Satraplatin and other derivatives; Topo I inhibitors, such as Camptothecin, Topotecan, irinotecan/SN38, rubitecan and other derivatives; Antimetabolites, such as Folic family (Methotrexate, Pemetrexed and relatives); Purine family (Thioguanine, Fludarabine, Cladribine, 6-Mercaptopurine and relatives); Pyrimidine family (Cytarabine, Gemcitabine, 5-Fluorouracil and relatives); Alkylating agents, such as Nitrogen mustards (Cyclophosphamide, Melphalan, Chlorambucil, mechlorethamine, Ifosfamide, and relatives); nitrosoureas (e.g. Carmustine); Triazenes (Dacarbazine, temozolomide); Alkyl sulphonates (e.g. Busulfan); Procarbazine and Aziridines; Antibiotics, such as Hydroxyurea; Anthracyclines (doxorubicin, daunorubicin, epirubicin and other derivatives); Anthracenediones (Mitόxantrone and relatives); Streptomyces family (Bleomycin, Mitomycin C, actinomycin) and Ultraviolet light. Biological Samples
[00240] As inhibitors of ATR kinase, the compounds and compositions of this invention are also useful in biological samples. One aspect of the invention relates to inhibiting ATR kinase activity in a biological sample, which method comprises contacting said biological sample with a compound described herein or a composition comprising said compound. The term "biological sample", as used herein, means an in vitro or an ex vivo sample, including, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof. The term "compounds described herein" includes compounds of formula I, II, III, IV, IA, HA, IHA, IVA, IA-Ϊ, IA-ii, IA-iii, V, VI, and VII.
[00241] Inhibition of ATR kinase activity in a biological sample is useful for a variety of purposes that are known to one of skill in the art. Examples of such purposes include, but are not limited to, blood transfusion, organ-transplantation, and biological specimen storage. Study of Protein Kinases
[00242] Another aspect of this invention relates to the study of protein kinases in biological and pathological phenomena; the study of intracellular signal transduction pathways mediated by such protein kinases; and the comparative evaluation of new protein kinase inhibitors. Examples of such uses include, but are not limited to, biological assays such as enzyme assays and cell-based assays.
[00243] The activity of the compounds as protein kinase inhibitors may be assayed in vitro, in vivo or in a cell line. In vitro assays include assays that determine inhibition of either the kinase activity or ATPase activity of the activated kinase. Alternate in vitro assays quantitate the ability of the inhibitor to bind to the protein kinase and may be measured either by radiolabelling the inhibitor prior to binding, isolating the inhibitor/kinase complex and determining the amount of radiolabel bound, or by running a competition experiment where new inhibitors are incubated with the kinase bound to known radioligands. Detailed conditions for assaying a compound utilized in this invention as an inhibitor of ATR is set forth in the Examples below.
[00244] Another aspect of the invention provides a method for modulating enzyme activity by contacting a compound described herein with ATR kinase.
Methods of Treatment
[00245] In one aspect, the present invention provides a method for treating or lessening the severity of a disease, condition, or disorder where ATR kinase is implicated in the disease state. In another aspect, the present invention provides a method for treating or lessening the severity of an ATR kinase disease, condition, or disorder where inhibition of enzymatic activity is implicated in the treatment of the disease. In another aspect, this invention provides a method for treating or lessening the severity of a disease, condition, or disorder with compounds that inhibit enzymatic activity by binding to the ATR kinase. Another aspect provides a method for treating or lessening the severity of a kinase disease, condition, or disorder by inhibiting enzymatic activity of ATR kinase with an ATR kinase inhibitor. [00246] One aspect of the invention relates to a method of inhibiting ATR kinase activity in a patient, which method comprises administering to the patient a compound described herein, or a composition comprising said compound. In some embodiments, said method is used to treat or prevent a condition selected from proliferative and hyperproliferative diseases, such as cancer.
[00247] Another aspect of this invention provides a method for treating, preventing, or lessening the severity of proliferative or hyperproliferative diseases comprising administering an effective amount of a compound, or a pharmaceutically acceptable composition comprising a compound, to a subject in need thereof. In some embodiments, said subject is a patient. The term "patient", as used herein, means an animal, preferably a human. [00248] In some embodiments, said method is used to treat or prevent cancer. In some embodiments, said method is used to treat or prevent a type of cancer with solid tumors. In yet another embodiment, said cancer is selected from the following cancers: Oral: buccal cavity, lip, tongue, mouth, pharynx; Cardiac: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma; Lung: bronchogenic carcinoma (squamous cell or epidermoid, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma; Gastrointestinal: esophagus (squamous cell carcinoma, larynx, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leiomyosarcoma), pancreas (ductal adenocarcinoma, insulinoma, glucagonoma, gastrinoma, carcinoid tumors, vipoma), small bowel or small intestines (adenocarcinoma, lymphoma, carcinoid tumors, Karposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma, fibroma), large bowel or large intestines (adenocarcinoma, tubular adenoma, villous adenoma, hamartoma, leiomyoma), colon, colon- rectum, colorectal; rectum, Genitourinary tract: kidney (adenocarcinoma, Wilm's tumor [nephroblastoma], lymphoma), bladder and urethra (squamous cell carcinoma, transitional cell carcinoma, adenocarcinoma), prostate (adenocarcinoma, sarcoma), testis (seminoma, teratoma, embryonal carcinoma, teratocarcinoma, choriocarcinoma, sarcoma, interstitial cell carcinoma, fibroma, fibroadenoma, adenomatoid tumors, lipoma); Liver: hepatoma (hepatocellular carcinoma), cholangiocarcinoma, hepatoblastoma, angiosarcoma, hepatocellular adenoma, hemangioma, biliary passages; Bone: osteogenic sarcoma (osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma, chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cell sarcoma), multiple myeloma, malignant giant cell tumor chordoma, osteochronfroma (osteocartilaginous exostoses), benign chondroma, chondroblastoma, chondromyxofibroma, osteoid osteoma and giant cell tumors; Nervous system: skull (osteoma, hemangioma, granuloma, xanthoma, osteitis deformans), meninges (meningioma, meningiosarcoma, gliomatosis), brain (astrocytoma, medulloblastoma, glioma, ependymoma, germinoma [pinealoma], glioblastoma multiform, oligodendroglioma, schwannoma, retinoblastoma, congenital tumors), spinal cord neurofibroma, meningioma, glioma, sarcoma); Gynecological: uterus (endometrial carcinoma), cervix (cervical carcinoma, pre-tumor cervical dysplasia), ovaries (ovarian carcinoma [serous cystadenocarcinoma, mucinous cystadenocarcinoma, unclassified carcinoma], granulosa- thecal cell tumors, Sertoli-Leydig cell tumors, dysgerminoma, malignant teratoma), vulva (squamous cell carcinoma, intraepithelial carcinoma, adenocarcinoma, fibrosarcoma, melanoma), vagina (clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma (embryonal rhabdomyosarcoma), fallopian tubes (carcinoma), breast; Skin: malignant melanoma, basal cell carcinoma, squamous cell carcinoma, Karposi's sarcoma, keratoacanthoma, moles dysplastic nevi, lipoma, angioma, dermatofibroma, keloids, psoriasis, Thyroid gland: papillary thyroid carcinoma, follicular thyroid carcinoma; medullary thyroid carcinoma, multiple endocrine neoplasia type 2A, multiple endocrine neoplasia type 2B, familial medullary thyroid cancer, pheochromocytoma, paraganglioma; and Adrenal glands: neuroblastoma.
[00249] In some embodiments, the cancer is selected from the cancers described herein. In some embodiments, said cancer is lung cancer, head and neck cancer, pancreatic cancer, gastric cancer, or brain cancer.
[00250] In certain embodiments, an "effective amount" of the compound or pharmaceutically acceptable composition is that amount effective in order to treat said disease. The compounds and compositions, according to the method of the present invention, may be administered using any amount and any route of administration effective for treating or lessening the severity of said disease.
[00251] One aspect provides a method for inhibiting ATR in a patient comprising administering a compound described herein as described herein. Another embodiment provides a method of treating cancer comprising administering to a patient a compound described herein, wherein the variables are as defined herein. [00252] Some embodiments comprising administering to said patient an additional therapeutic agent selected from a DNA-damaging agent; wherein said additional therapeutic agent is appropriate for the disease being treated; and said additional therapeutic agent is administered together with said compound as a single dosage form or separately from said compound as part of a multiple dosage form.
[00253] In some embodiments, said DNA-damaging agent is selected from ionizing radiation, radiomimetic neocarzinostatin, a platinating agent, a Topo I inhibitor, a Topo II inhibitor, an antimetabolite, an alkylating agent, an alkyl sulphonates, an antimetabolite, or an antibiotic. In other embodiments, said DNA-damaging agent is selected from ionizing radiation, a platinating agent, a Topo I inhibitor, a Topo II inhibitor, or an antibiotic.
[00254] Examples of Platinating agents include Cisplatin, Oxaliplatin, Carboplatin,
Nedaplatin, Satraplatin and other derivatives. Other platinatirfg agents include Lobaplatin, and Triplatin. Other platinating agents include Tetranitrate, Picoplatin, Satraplatin,
ProLindac and Aroplatin.
[00255] Examples of Topo I inhibitor include Camptothecin, Topotecan, irinotecan/SN38, rubitecan and other derivatives. Other Topo I inhibitors include Belotecan.
[00256] Examples of Topo II inhibitors include Etoposide, Daunorubicin, Doxorubicin,
Aclarubicin, Epirubicin, Idarubicin, Amrubicin, Pirarubicin, Valrubicin, Zorubicin and
Teniposide.
[00257] Examples of Antimetabolites include members of the Folic family, Purine family
(purine antagonists), or Pyrimidine family (pyrimidine antagonists). Examples of the Folic family include methotrexate, pemetrexed and relatives; examples of the Purine family include Thioguanine, Fludarabine, Cladribine, 6-Mercaptopurine, and relatives; examples of the Pyrimidine family include Cytarabine, gemcitabine, 5-Fluorouracil (5FU) and relatives.
[00258] Some other specific examples of antimetabolites include Aminopterin,
Methotrexate, Pemetrexed, Raltitrexed, Pentostatin, Cladribine, Clofarabine, Fludarabine,
Thioguanine, Mercaptopurine, Fluorouracil, Capecitabine, Tegafur, Carmofur, Floxuridine,
Cytarabine, Gemcitabine, Azacitidine and Hydroxyurea.
[00259] Examples of alkylating agents include Nitrogen mustards, Triazenes, alkyl sulphonates, Procarbazine and Aziridines. Examples of Nitrogen mustards include
Cyclophosphamide, Melphalan, Chlorambucil and relatives; examples of nitrosoureas include Carmustine; examples of triazenes include Dacarbazine and temozolomide; examples of alkyl sulphonates include Busulfan.
[00260] Other specific examples of alkylating agents include Mechlorethamine, Cyclophosphamide, Ifosfamide, Trofosfamide, Chlorambucil, Melphalan, Prednimustine, Bendamustine, Uramustine, Estramustine, Carmustine, Lomustine, Semustine, Fotemustine, Nimustine, Ranimustine, Streptozocin, Busulfan, Mannosulfan, Treosulfan, Carboquone, ThioTEPA, Triaziquone, Triethylenemelamine, Procarbazine, Dacarbazine, Temozolomide, Altretamine, Mitobronitol, Actinomycin, Bleomycin, Mitomycin and Plicamycin. [00261] Examples of antibiotics include Mitomycin, Hydroxyurea; Anthracyclines, Anthracenediones, Streptomyces family. Examples of Anthracyclines include doxorubicin, daunorubicin, epirubicin and other derivatives; examples of Anthracenediones include Mitoxantrone and relatives; examples of Streptomyces family inclue Bleomycin, Mitomycin C, and actinomycin.
[00262] In certain embodiments, said platinating agent is Cisplatin or Oxaliplatin; said Topo I inhibitor is Camptothecin; said Topo Il inhibitor is Etoposide; and said antibiotic is Mitomycin. In other embodiments, said platinating agent is selected from Cisplatin, Oxaliplatin, Carboplatin, Nedaplatin, or Satraplatin; said Topo I inhibitor is selected from Camptothecin, Topotecan, irinotecan/SN38, rubitecan; said Topo II inhibitor is selected from Etoposide; said antimetabolite is selected from a member of the Folic Family, the Purine Family, or the Pyrimidine Family; said alkylating agent is selected from nitrogen mustards, nitrosoureas, triazenes, alkyl sulfonates, Procarbazine, or aziridines; and said antibiotic is selected from Hydroxyurea, Anthracyclines, Anthracenediones, or Streptomyces family. [00263] Another embodiment provides a method of promoting cell death in cancer cells comprising administering to a patient a compound described herein, , or a composition comprising said compound.
[00264] Yet another embodiment provides a method of preventing cell repair of DNA damage in cancer cells comprising administering to a patient a compound described herein, or a composition comprising said compound. Yet another embodiment provides a method of preventing cell repair caused by of DNA damage in cancer cells comprising administering to a patient a compound of formula I, or composition comprising said compound. [00265] Another embodiment provides a method of sensitizing cells to DNA damaging agents comprising administering to a patient a compound described herein, or a composition comprising said compound.
[00266] In some embodiments, the method is used on a cancer cell having defects in the ATM signaling cascade. In some embodiments, said defect is altered expression or activity of one or more of the following: ATM, p53, CHK2, MREl 1 , RAD50, NBSl, 53BP1, MDCl or H2AX. In another embodiment, the cell is a cancer cell expressing DNA damaging oncogenes. In some embodiments, said cancer cell has altered expression or activity of one or more of the following: K-Ras, N-Ras, H-Ras, Raf, Myc, Mos, E2F, Cdc25A, CDC4, CDK2, Cyclin E, Cyclin A and Rb.
[00267] Yet another embodiment provides use of a compound described herein as a radio- sensitizer or a chemo-sensitizer.
[00268] Yet other embodiment provides use of a compound of formula I as a single agent (monotherapy) for treating cancer. In some embodiments, the compounds of formula I are used for treating patients having cancer with a DNA-damage response (DDR) defect. In other embodiments, said defect is a mutation or loss of ATM, p53, CHK2, MREl 1, RAD50, NBSl, 53BP1, MDCl, or H2AX.
SCHEMES
[00269] The compounds of the disclosure may be prepared in light of the specification using steps generally known to those of ordinary skill in the art. Those compounds may be analyzed by known methods, including but not limited to LCMS (liquid chromatography mass spectrometry) and NMR (nuclear magnetic resonance). Below are a set of generic schemes that illustrate generally how to prepare the compounds of the present disclosure. Scheme I-Al : Preparation of Compounds wherein -L-R1 is an Aromatic Amide
Figure imgf000154_0001
[00270] Cyclic amides compounds of the present disclosure wherein -L-R1 is an aromatic amide can be prepared according to methods similar to the one depicted in Scheme I-Al : Commercially available ester 1 is reacted with a boronic acid under Suzuki conditions to give intermediate 2. The carboxylic acid group is engaged in a coupling reaction with an amine to lead to cyclic amide compounds of the Formula I.
Scheme I-A2: Preparation of Compounds wherein -L-R1 is an Aromatic Amide
Figure imgf000155_0001
[00271] Alternatively, compounds of the present disclosure wherein -L-R1 is an aromatic amide can be prepared according to methods similar to the one depicted in Scheme 1-A2, a variation of the synthetic sequence depicted in scheme I-Al which consists in starting from methyl ester 1. Ester 1. is transformed into carboxylic acid 3 which is engaged in a coupling reaction with an amine to give amide 4. This is reacted with a boronic acid under Suzuki conditions to lead to compounds of formula I.
Scheme I-Bl : preparation of compounds where Ring A is a 1 ,3,4-oxadiazole
Figure imgf000155_0002
wherein R is -(L-NR1ROp or -(J2)q
[00272] Compounds of the present disclosure where Ring A is a 1 ,3,4-oxadiazole can be prepared according to methods similar to the one depicted in Scheme I-Bl : methyl ester 3 is reacted with a boronic acid under Suzuki conditions to give intermediate 8. The carboxylic acid in 8 is then engaged into a coupling reaction with an hydrazide (X=O) or thiohydrazide (X=S) to form 9 . Finally, the the acylhydrazide in 9 undergoes a cyclodehydration to lead to compounds of the present disclosure (formula I in Scheme I-Bl). Transformation of intermediate 8 into compounds of formula I has also been performed in a one-pot procedure using reagents serving two purposes (coupling and cyclodehydration).
Scheme I-B2: preparation of compounds where Ring A is a 1 ,3,4-oxadiazole
Figure imgf000156_0001
wherein R is -(L-NR1ROp or -(J2),
[00273] Alternatively, compounds of the present disclosure where Ring A is a 1,3,4- oxadiazole can be prepared according to methods similar to the one depicted in Scheme I-B2, a variation of the synthetic sequence depicted in scheme I-Bl . The hydrazide 5 is engaged in a coupling reaction with a carboxylic acid functional group to form intermediate 9 (X=O). As in scheme I-Bl the acylhydrazide then undergoes a cyclodehydration to lead to compounds of formula I. When R5 is a moiety bound to the oxadiazole ring through a C-N bond, then an thioisocyanate can be used to generate intermediate 9 (X=S); the thioacylhydrazide then undergoes a cyclodehydration to lead to compounds of formula I.
Scheme I-B3: preparation of compounds where Ring A is a 1,3,4-oxadiazole
Figure imgf000156_0002
wherein R is -(L-NR1 R2)p or ~{h\ [00274] Alternatively, compounds of the present disclosure where Ring A is 1 ,3,4- oxadiazole can be prepared according to methods similar to the one depicted in Scheme I-B3: the R functional group in IO or 6 (acid and hydrazide respectively, both prepared from methyl ester 3 through hydrolysis and hydrazinolysis respectively) are engaged into coupling with a suitable partner (R5CXNHNH2 when starting from 10; R5COOH/R5==S when starting from 6) to form acylhydrazide intermediate 11. Subsequent cyclodehydration leads to the compound 12 where the 1 ,3,4-oxadiazole ring has been constructed. Transformation of starting point 10 or_6 into intermediate \2 has also been performed in a one-pot procedure using reagents serving two purposes (coupling and cyclodehydration). The bromo handle in oxadiazole 12 is then reacted with a boronic acid under Suzuki conditions to give compounds of formula I. When R group in I contains a carboxylic acid moiety, it can be further transformed (eg into an amide) using conditions known in the art.
Scheme I -Cl : preparation of compounds where Ring A is a 1 ,2,4-oxadiazole
Figure imgf000157_0001
wherein R is -(L-NR1ROp or -{J2)q
[00275] Compounds of the present disclosure where Ring A is a 1 ,2,4-oxadiazole can be prepared according to methods similar to the one depicted in Scheme I-Cl : nitrile 2 reacts with hydroxylamine to give intermediate 13. The hydroxy group in 13 reacts with acid chlorides to lead to intermediate 14 which undergoes cyclodehydration to afford compounds of formula I.
Scheme I-C2: preparation of compounds where Ring A is a 1 ,2,4-oxadiazole
Figure imgf000158_0001
wherein R is -(L-NR'R2)P or -(J2^
[00276] Alternatively, compounds of the present disclosure where Ring A is a 1 ,2,4- oxadiazole can be prepared according to methods similar to the one depicted in Scheme I-C2: Commercially available nitrile 1 reacts with hydroxylamine to give intermediate 1J5. The hydroxy group in 15 reacts with acid chlorides to lead to intermediate .16 which undergoes cyclodehydration to afford intermediate 12- The bromo handle in 17 is then used to perform a Suzuki reaction with a boronic acid coupling partner to give compounds of formula I. When R group in I contains a carboxylic acid moiety, it can be further transformed (eg into an amide) using conditions known in the art.
Scheme I-Dl : preparation of compounds where Ring A is a 1,3,4-thiadiazole
Figure imgf000158_0002
wherein R is -(L-NR1R^p or -<J2)q
[00277] Compounds of the present disclosure where Ring A is a 1,3,4-thiadiazole can be prepared according to methods similar to the one depicted in Scheme I-Dl : methyl ester 3 is reacted with a boronic acid under Suzuki conditions to give intermediate 8. The carboxylic acid in 8 is then engaged into a coupling reaction with a thiohydrazide to form 18. Finally, the the thioacylhydrazide in 18 undergoes a cyclodehydration to lead to compounds of formula I. Transformation of intermediate 8 into compounds of formula I can be performed in a one-pot procedure using reagents serving two purposes (coupling and cyclodehydration)
Scheme I-D2: preparation of compounds where Ring A is a 1,3,4-thiadiazole
Figure imgf000159_0001
wherein R is -(L-NR l 'πR> 2^N)p or -(J2),
[00278] Alternatively, compounds of the present disclosure where Ring A is 1,3,4- thiadiazole can be prepared according to methods similar to the one depicted in Scheme I- D2: the acid functional group in 10 is engaged into coupling with a suitable partner (R5CSNHNH2) to form the thioacylhydrazide intermediate ,19. Subsequent cyclodehydration leads to the compound 20 where the 1,3,4-thiadiazole ring has been constructed. Transformation of starting point 10 into 20 has been performed in a one-pot procedure using reagents serving two purposes (coupling and cyclodehydration). The bromo handle in thiadiazole 20 is then reacted with a boronic acid under Suzuki conditions to give compounds of formula I. When R group in I contains a carboxylic acid moiety, it can be further transformed (eg into an amide) using conditions known in the art.
Scheme I -El : preparation of compounds where Ring A is an isoxazole
Figure imgf000159_0002
wherein R is -(L-NR1R2)p or -(J2)q [00279] Compounds of the present disclosure where Ring A is an isoxazole can be prepared according to methods similar to the one depicted in Scheme I-El : Commercially available 2-amino-3,5-dibromo pyrazine 21_ undergoes a Sonogashira coupling with TMS- acetylene to give intermediate 22, the amino group of which can be fully protected as the diBoc species 23 . A Suzuki coupling with the remaining bromo handle, with concommitent TMS deprotection affords intermediate 24. The alkyne 24 finally reacts in a cyclocondensation with N-hydroxyaroyl chloride to furnish compounds of Formula I.
Scheme I-E2: preparation of compounds where Ring A is an isoxazole
Figure imgf000160_0001
wherein R is -(L-NR'R2)P or -(J2)q
[00280] Alternatively, compounds of the present disclosure where Ring A is an isoxazole can be prepared according to methods similar to the one depicted in Scheme I-E2: The TMS- protected intermediate 23, described in scheme I-El can be deprotected to reveal the alkyne compound 25. The alkyne 25 reacts in a cyclocondensation with N-hydroxyaroyl chloride to furnish intermediate 26 where the isoxazole ring has been constructed. The bromo handle in isoxazole 26 is then reacted with a boronic acid under Suzuki conditions to give compounds 27. A final deprotection of N-protecting groups in 27 can reveal compounds of Formula I. When R group in I contains a carboxylic acid moiety, it can be further transformed (eg into an amide) using conditions known in the art.
Scheme 1-Fl : preparation of compounds where Ring A is a 1,2,4-triazoIe
Figure imgf000161_0001
wherein R is -(L-NR'R2)P Or -(J2^
[00281] Alternatively, compounds of the present disclosure where Ring A is a 1,2,4- triazole can be prepared according to methods similar to the one depicted in Scheme I-Fl starting from methyl ester 3. Ester 3 is reacted with a boronic acid under Suzuki conditions to give intermediate 4. When R group contains a carboxylic acid moiety, it can be further . transformed at this stage (eg into an amide) using conditions known in the art. The methyl ester group in 4 is then transformed into an hydrazide by reaction with hydrazine to give 5. Finally, the hydrazide group in 5 is engaged in a coupling reaction with a nitrile and subsequently undergoes a cyclodehydration to lead to compounds of formula I.
Scheme 1-F2: preparation of compounds where Ring A is a 1,2,4-triazole
Figure imgf000161_0002
wherein R is -(L-NR1 R2)p or -(J2)q
[00282] Alternatively, compounds of the present disclosure where Ring A is a 1 ,2,4- triazole can be prepared according to methods similar to the one depicted in Scheme I-F2: the R functional group in I- or 3 (nitrile and methyl ester respectively) are engaged into coupling (after appropriate transformation of 3 into hydrazide 6) with a suitable coupling partner (R5CONHNH2 when starting from 1; R5CN if using 6). Subsequent cyclodehydration leads to the intermediate 7 where the 1,2,4-triazole ring has been constructed.. The bromo handle in triazole 7 is then reacted with a boronic acid under Suzuki conditions to give compounds of formula I. When R group in I contains a carboxylic acid moiety, it can be further transformed (eg into an amide) using conditions known in the art.
Scheme I-Gl : preparation of compounds where Ring A is a benzoxazole
Figure imgf000162_0001
wherein R is -(L-NR1R2Jp or -(J2)q
[00283] Benzoxazole compounds of Formula VI can be prepared according to methods similar to the one depicted in Scheme I-Gl : Commercially available nitrile 1 is reacted with a amino phenol to give the benzoxazole which is then reacted with a boronic acid under Suzuki conditions to give compounds of the formula VI.
Scheme I-Hl : preparation of compounds where Ring A is a benzothiazole
Figure imgf000162_0002
Vl wherein R is -(L-NR1R2Jp Or -(J2),
[00284] Benzothiazole compounds of Formula VI can be prepared according to methods similar to the one depicted in Scheme I-Hl : Commercially available nitrile J, is reacted with a aminobenzenethiol to give the benzothiazole which is then reacted with a boronic acid under Suzuki conditions to give compounds of the formula VI. Scheme I-H2: preparation of compounds where benzothiazole
Figure imgf000163_0001
Vl wherein R is -(L-NR1R^p or -iJ2)q
[00285] Alternatively, benzothiazole compounds of Formula Vl can be prepared according to Scheme I-H2; methyl ester 3 is reacted with a boronic acid under Suzuki conditions to give intermediate 8. Cyclisation of intermediate 8 with an amino benzenethiol will lead to compounds of the formula VI.
Scheme I— 11 : preparation of compounds where Ring A is an imidazole
Suzuki
Figure imgf000163_0002
wherein R is -(L-NR1R2),, or -CJ2)q
[00286] Benzimidazole compounds of Formula I can be prepared according to methods similar to the one depicted in Scheme I-Il : methyl ester 3 is reacted with a boronic acid under Suzuki conditions to give intermediate 8. Cyclisation of internediate 8 with a benzene 1 ,2-diamine will lead to compounds of the formula I
Scheme 1-12: preparation of compounds where Ring A is an imidazole Suzuki
Figure imgf000164_0001
3 9 I wherein R is -(L-NR'R2)P or -(J2)q
[00287] Alternatively, benzimidazole compounds of Formula I can be prepared according to methods similar to the one depicted in Scheme 1-12: Reaction of the acid functional group of 3 is reacted with a benzene 1,2-diamine to give the benzimidazole intermediate 9. Intermediate 9 is then reacted with a boronic acid under Suzuki conditions to give compounds of the formula I.
EXAMPLES
[00288] It should be understood that the specific conditions shown below are only examples, and are not meant to limit the scope of the conditions that can be used for making, analyzing, or testing the compounds of the disclosure. Instead, this invention also includes conditions known to those skilled in that art for making, analyzing, and testing the compounds of the disclosure.
HPLC Methods
[00289] As used herein, the term "Rt(min)" refers to the HPLC retention time, in minutes, associated with the compound. Unless otherwise indicated, the HPLC method utilized to obtain the reported retention time is as follows:
Column: ACE C8 column, 4.6 x 150 mm
Gradient: 0-100% acetonitrile+methanol 60:40 (2OmM Tris phosphate)
Flow rate: 1.5 mL/minute
Detection: 225 nm. HNMR Methods [00290] 1H-NMR spectra were recorded at 400 MHz using a Bruker DPX 400 instrument. Mass Spectrometry Methods
[00291] Mass spec, samples were analyzed on a MicroMass Quattro Micro mass spectrometer operated in single MS mode with electrospray ionization. Samples were introduced into the mass spectrometer using chromatography. Mobile phase for all mass . spec, analyses consisted of 1OmM pH 7 ammonium acetate and a 1 :1 acetonitrile-methanol mixture, column gradient conditions are 5%-100% acetonitrile-methanol over 3.5 mins gradient time and 5 mins run time on an ACE C8 3.0 x 75mm column. Flow rate is 1.2 ml/min.
[00292] The following compounds were prepared and analyzed as follows.
Example 1 : 3-amino-6-(4-methoxyphenylVN-phenylpyrazine-2-carboxarnide (Compound
LU
Figure imgf000165_0001
SCHEME A
Figure imgf000165_0002
METHOD A
Step 1 : Methyl 3-amino-6-bromopyrazine-2-carboxylate
Figure imgf000165_0003
[00293] A mixture of methyl 3-aminopyrazine-2-carboxylate (8.35 g, 54.53 mmol) and N- bromo-succinimide (9.705 g, 54.53 mmol) was stirred in MeCN (100 mL) at room temp overnight. The resultant precipitate was filtered, washed with MeCN and dried to give the desired product as a yellow solid (1 1.68 g, 92% Yield)
[00294] 1H NMR (400.0 MHz, DMSO) 3.85 (s, 3H), 7.55 (br s, 2H) and 8.42 (s, 1H) ppm; MS (ES+) 233
Step 2 : S-amino-ό-bromopyrazine-^-carboxylic acid
Figure imgf000166_0001
[00295] A mixture of methyl 3-amino-6-bromo-pyrazine-2-carboxylate (5.1 1 g, 22.02 mmol) and lithium hydroxide (2.637 g, 1 10.1 mmol) in MeOH (20 mL) and H2O (20 mL) was heated to 90 °C for 2 hours. The reaction mixture was allowed to cool and neutralised with HCl and the resultant precipitate collected by filtration. Taken on to the next step without further purification (4.8Og, 99% Yield).
[00296] Step 3 : S-amino-ό-bromo-N-phenylpyrazine-1-carboxamide
Figure imgf000166_0002
[00297] A mixture of 3-amino-6-bromo-pyrazine-2-carboxylic acid (3.5 g, 16.05 mmol), l ,l'-carbonyldiimidazole (5.205 g, 32.10 mmol), DIPEA (2.282 g, 3.075 mL, 17.66 mmol) and DMAP (98.04 mg, 0.8025 mmol) were combined in DMSO (131.2 mL) and stirred for 30 min. Aniline (1.495 g, 1.463 mL, 16.05 mmol) was then added and the resulting solution stirred at RT for 18 hours. After this time water was added and the product collected by filtration to give a brown powder (3.5 g, 74% Yield).
[00298] 1H NMR (400.0MHz, DMSO) d 7.04 (1H, m), 7.29 (2H, m), 7.72 (4H, m), 8.36 (1H, s), 10.22 (NH2) ppm; MS (ES+) 295. [00299] Step 4 : 3-amino-6-(4-methoxyphenyl)-N-phenylpyrazine-2-carboxamide (Compound 1-1)
Figure imgf000167_0001
[00300] A Greenhouse tube was charged with 4-Methoxyphenylboronic acid (31.4 mg, 0.207 mmol) and treated with a solution of dichloropalladium;triphenylphosphane (4.84 mg, 0.0069 mmol) and 3-amino-6-bromo-N-phenyl-pyrazine-2-carboxamide (40.45 mg, 0.138 mmol) in DMF (0.81 mL) followed by Na2CO3 (2M solution, 207uL, 0.414 mmol). The mixture was flushed with nitrogen and heated to 88 °C for 18 hours. After this time [00301] the reaction was filtered to remove inorganics and the resultant residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, lOuM, IOOA column, gradient 10% - 95%B (solvent A: 0.05% TFA in water, solvent B: CH3CN) over 16 minutes at 25mL/min]. The fractions were freeze-dried to give the title compound as a solid (18.56mg, 38% Yield). MS (ES+) 321
Compounds 1-1 to 1-41 were prepared using Method A.
Compound 1-2 3-amino-6-(3-cyanopyridin-4-yl)-N-phenylpyrazine-2-carboxamide
I H ΝMR (400.0 MHz, DMSO) d 7.17 (t, J = 7.3 Hz, I H), 7.39 - 7.43 (m, 2H), 7.81 - 7.83
(m, 2H), 8.30 (d, J = 5.4 Hz, 2H), 8.40 (s, I H), 8.91 (d, J = 5.5 Hz, 1H), 9.13 (s, 1H), 9.17 (s,
1 H) and 10.16 (s, 1 H) ppm; MS (ES+) 317
Compound 1-3 3-amino-Ν-phenyl-6-(4-(2-(piperidin-1-yl) ethylcarbamoyl)phenyl) pyrazine-2-carboxamide
I H NMR (400.0 MHz, DMSO) d 3.35 (s, 3H), 7.17 (t, J = 7.4 Hz, I H), 7.43 - 7.39 (m, 2H),
7.78 (t, J = 7.8 Hz, 2H), 7.82 (d, J = 7.7 Hz, 2H), 7.92 - 7.94 (m, I H), 8.60 - 8.66 (m, 2H),
9.05 (s, 1 H) and 10.50 (s, 1 H) ppm; MS (ES+) 369.
Compound 1-4 3-amino-6-(4-fluorophenyl)-N-phenylpyrazine-2-carboxamide 1H NMR (400.0 MHz, DMSO) d 7.16 (t, J = 7.3 Hz, 1H), 7.32 (t, J = 8.9 Hz, 2H), 7.38 -
7.42 (m, 2H), 7.69 (s, 2H), 7.81 - 7.83 (m, 2H), 8.28 - 8.31 (m, 2H), 8.92 (s, 1H), 10.42 (s,
1H) ppm; MS (ES+) 309
Compound 1-5 3-amino-6-(4-(methylsulfonamido)phenyl)-N-phenylpyrazine-2- carboxamide
1H NMR (400.0 MHz, DMSO) d 7.16 (t, J = 7.4 Hz, I H), 7.33 (d, J = 8.7 Hz, 2H), 7.38 -
7.42 (m, 2H), 7.65 (s, 2H), 7.83 (d, J = 7.6 Hz, 2H), 8.21 (d, J = 8.7 Hz, 2H), 8.90 (s, 1H),
9.92 (s, I H), 10.37 (s, I H) ppm; MS (ES+) 384
Compound 1-6 3-amino-N-phenyl-6-(2-(trifluoromethyl)phenyl)pyrazine-2-carboxamide I H
NMR (400.0 MHz, DMSO) d 7.1 1 - 7.16 (m, 1H), 7.36 - 7.40 (m, 2H), 7.69 - 7.72 (m, 3H),
7.80 - 7.84 (m, 4H), 7.93 (d, J = 7.8 Hz, 1H), 8.52 (s, 1H), 10.12 (s, I H) ppm; MS (ES+) 359
Compound 1-7 4-(5-amino-6-(phenylcarbamoyl)pyrazin-2-yl)benzoic acid
1H NMR (400 MHz, DMSO) 7.17 (1H, t), 7.41 (2H, t), 7.83 (4H, d), 8.03 (2H, d), 8.37 (2H, d), 9.01 (1H, s), 10.45 (1H, s), 13.03 (1H, br s) ppm; MS (ES+) 335
Compound 1-8 3-(5-amino-6-(phenylcarbamoyl)pyrazin-2-yl)benzoic acid
1H NMR (400 MHz, DMSO) 7.16 (1H, t), 7.38-7.42 (3H, m), 7.64 (2H, br s), 7.81 (2H, d),
7.88 (1H, d), 8.17 (I H, d), 8.46 (1H, d), 8.85 (1H, s), 10.39 (1H, s) ppm; MS (ES+) 335
Compound 1-9 3-amino-6-(3-fluorophenyl)-N-phenylpyrazine-2-carboxarnide
1H NMR (400.0 MHz, DMSO) d 7.15 - 7.25 (m, 2H), 7.40 (dd, J = 1.7, 15.9 Hz, I H), 7.41
(s, I H), 7.52 (td, J = 8.0, 4.7 Hz, 1H), 7.80 - 7.82 (m, 4H), 8.06 (d, J = 8.0 Hz, 8.17 - 8.20 (m,
1H), 8.97 (s, 1H), 10.46 (s, 1H) ppm; MS (ES+) 309
Compound 1-10 3-amino-6-(3-cyanophenyl)-N-phenylpyrazine-2-carboxamide
MS (ES+) 316
Compound 1-11 3-amino-N-phenyl-6-o-tolylpyrazine-2-carboxamide
MS (ES+) 305
Compound 1-12 3-amino-6-(3-morpholinopheny!)-N-phenylpyrazine-2-carboxarnide; MS
(ES+) 376
Compound 1-13 3-amino-6-(4-morpholinophenyl)-N-phenylpyrazine-2-carboxamide MS
(ES+) 376
Compound 1-14 3-amino-6-(2-fluorophenyl)-N-phenylpyrazine-2-carboxamide
MS (ES+) 309 Compound 1-15 3-amino-N,6-diphenylpyrazine-2-carboxamide; MS (ES+) 291
Compound 1-16 3-amino-6-(4-(hydroxymethyl)phenyl)-N-phenylpyrazine-2-carboxamide;
MS (ES+) 321
Compound 1-17 6-(4-acetylphenyl)-3-amino-N-phenylpyrazine-2-carboxamide
MS (ES+) 333
Compound 1-18 3-amino-6-(3-carbamoylphenyl)-N-phenylpyrazine-2-carboxamide; MS
(ES+) 334
Compound 1-19 3-amino-6-(2-(hydroxymethyl)phenyl)-N-phenylpyrazine-2-carboxamide;
MS (ES+) 321
Compound 1-20 3-amino-6-(3-(morpholine-4-carbonyl)phenyl)-N-phenylpyrazine-2- carboxamide; MS (ES+) 404
Compound 1-21 3-amino-6-(4-cyanophenyl)-N-phenylpyrazine-2-carboxamide
MS (ES+) 316
Compound 1-22 6-(3-acetylphenyl)-3-amino-N-phenylpyrazine-2-carboxamide
MS (ES+) 333
Compound 1-23 3-amino-6-(4-(2-(4-hydroxypiperidin-1-yl)acetyl)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 432
Compound 1-24 3-amino-6-(4-(dimethylcarbamoyl)phenyl)-N-phenylpyrazine-2- carboxamide; MS (ES+) 362
Compound 1-25 3-amino-6-(3-(methylsulfonamido)phenyl)-N-phenylpyrazine-2- carboxamide; MS (ES+) 384
Compound 1-26 3-amino-6-(3-(morpholine-4-carbonyl)phenyl)-N-(4-(pyrrolidin-1- yl)phenyl)pyrazine-2-carboxamide; MS (ES+) 473
Compound 1-27 3-amino-6-(3-carbamoylphenyl)-N-(2-methoxyphenyl)pyrazine-2- carboxamide; MS (ES+) 364
Compound 1-28 3-iamino-6-(4-(dimethylcarbamoyl)phenyl)-N-(2-methoxyphenyl) pyrazine-
2-carboxamide; MS (ES+) 392
Compound 1-29 3-amino-6-(lH-indol-5-yl)-N-(2-methoxyphenyl)pyrazine-2-carboxamide
1 H ΝMR (400.0 MHz, DMSO) d 4.03 (s, 3H), 6.55 (d, J = 1.9 Hz, 1 H), 7.03 - 7.05 (m, 1 H),
7.13 - 7.19 (m, 2H), 7.43 (t, J = 2.7 Hz, 1 H), 7.55 (d, J = 8.6 Hz, 2H), 7.87 (dd, J = 1.6, 8.6 Hz, 1H), 8.31 (s, I H), 8.39 (dd, J = 1.4, 7.9 Hz, 1H), 8.99 (s, 1H), 10.85 (s, 1H) and 1 1.27 (s,
1H) ppm; MS (ES+) 360
Compound 1-30 3-amino-6-(furan-2-yl)-N-(2-methoxyphenyl)pyrazine-2-carboxamide 1H
NMR (400.0 MHz, DMSO) d 3.98 (s, 3H), 6.56 (s, 1H), 6.69 (s, I H), 7.00 - 7.03 (m, 2H),
7.15 (s, 1 H), 7.86 (br s, 2H), 7.86 (s, 1 H), 8.32 (d, 1 H), 8.72 (s, 1 H) and 10.51 (s, 1 H) ppm;
MS (ES+) 31 1
Compound 1-31 3-amino-N-phenyl-6-(1H-pyrazol-5-yl)pyrazine-2-carboxamide
I H NMR (400.0 MHz, DMSO) d 6.98 (d, J = 10.5 Hz, I H), 7.18 (t, J = 7.4 Hz, I H), 7.40 -
7.44 (m, 2H), 7.67 (s, 3H), 7.81 (d, J = 7.7 Hz, 2H), 8.83 (s, 1H), 10.54 (s, I H) and 13.80 (s,
I H) ppm; MS (ES+) 281
Compound 1-32 3-amino-6-(6-hydroxypyridin-3-yl)-N-phenylpyrazine-2-carboxamide 1H
NMR (400.0 MHz, DMSO) d 6.45 (d, J = 9.6 Hz, I H), 7.14 - 7.18 (m, 1H), 7.38 - 7.42 (m,
2H), 7.58 (s, 2H), 7.78 - 7.80 (m, 2H), 8.31 (d, J = 2.5 Hz, I H), 8.39 (dd, J = 2.6, 9.6 Hz,
1H), 8.79 (s, 1H), 10.42 (s, I H) and 12.00 (s, 1H) ppm; MS (ES+) 308
Compound 1-33 3-amino-N-phenyl-6-(pyridin-4-yl)pyrazine-2-carboxamide
1H NMR (400.0 MHz, DMSO) d 7.18 (t, J = 7.5 Hz, 1H), 7.41 (dd, J = 1.8, 14.1 Hz, 2H),
7.82 (dd, J = 0.8, 8.4 Hz, 2H), 7.90 (s, 2H), 8.25 (dd, J = 1.6, 4.6 Hz, 2H), 8.67 (dd, J = 1.4,
4.8 Hz, 2H), 9.07 (s, I H) and 10.48 (s, 1H) ppm; MS (ES+) 292
Compound 1-34 3-amino-6-(6-morpholinopyridin-3-yl)-N-phenylpyrazine-2-carboxamide;
MS (ES+) 377
Compound 1-35 3-amino-N-(2-methoxyphenyl)-6-(thiophen-2-yl)pyrazine-2-carboxamide;
MS (ES+) 327
Compound 1-36 3-amino-6-(lH-indazol-5-yl)-N-(2-methoxyphenyl)pyrazine-2- carboxamide; MS (ES+) 361
Compound 1-37 3-amino-6-(furan-3-yl)-N-(2-methoxyphenyl)pyrazine-2-carboxamide; MS
(ES+) 31 1
Compound 1-38 3-amino-6-(2-methoxypyridin-4-yl)-N-phenylpyrazine-2-carboxamide; MS
(ES+) 322
Compound 1-39 3-amino-6-(lH-indazol-5-yl)-N-phenylpyrazine-2-carboxamide
MS (ES+) 331
Compound 1-40 3-amino-N-phenyl-6-(pyrimidin-5-yl)pyrazine-2-carboxamide MS (ES+) 293
Compound 1-41 3-amino-6-(furan-2-yl)-N-phenylpyrazine-2-carboxamide
MS (ES+) 281
Example 2 : (RV3-amino-Ν-phenvI-6-(4-(2-(pyrrolidin-1-ylmethyl)pyrrolidine-1- carbonyl)phenyl)pyrazine-2-carboxamide (-Compound 1-42)
SCHEME B
Figure imgf000171_0001
[00302] Compound 1-42 was prepared by using Method A, Steps 1-3 followed by Method I-B, Steps 1-2.
METHOD I-B
Step 1 : 3-(5-amino-6-(phenylcarbamoyl)pyrazin-2-yl)benzoic acid
Figure imgf000171_0002
[00303] A mixture of 3-amino-6-bromo-N-phenyl-pyrazine-2-carboxamide (2.5 g, 8.529 mmol), 3-boronobenzoic acid (1.415 g, 8.527 mmol) and Na2CO3 (1.808 g, 17.06 mmol) was suspended in MeCN (40 mL) / water (40 mL). The mixture was degassed (5 x N 2 vacuum cycles) and Pd(PPh3)4 (985.6 mg, 0.8529 mmol) added. The mixture was degassed again and heated to 9O°C. After 2 hours, the mixture was allowed to cool and concentrated to half its original volume. The resulting yellow precipitate was collected and washed with DCM and water (3.05g, 86% Yield). 1H NMR (400 MHz, DMSO) d 7.16 (1H, t), 7.38-7.42 (3H, m), 7.64 (2H, br s), 7.81 (2H, d), 7.88 (1H, d), 8.17 (1H, d), 8.46 (I H, d), 8.85 (1H, s), 10.39 (1H, s) ppm; MS (ES+) 335
Step 2: (R)-3-amino-N-pheny l-6-(4-(2-(pyrrolidin- 1 -ylmethy l)pyrrolidine- 1 - carbonyl)phenyl)pyrazine-2-carboxamide
Figure imgf000172_0001
[00304] l-[[(2R)-pyrrolidin-2-yl]methyl]pyrrolidine (69.23mg, 0.449 mmol) was weighed into a greenhouse tube and treated with a solution of 3-(5-amino-6-
(phenylcarbamoyl)pyrazin-2-yl)benzoic acid (50mg, 0.150 mmol), CDI (48.5 lmg, 0.299 mmol) and DMAP (1.82mg, 0.015 mmol) in DMSO (ImL of a stock solution). DIPEA
(78.2uL, 0.449 mmol) was then added and the mixture stirred at 38°C for 6 hours. The reaction mixture was filtered and the resultant residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, lOuM, IOOA column, gradient 10% - 95%B (solvent
A: 0.05% TFA in water, solvent B: CH3CN) over 16 minutes at 25mL/min]. The fractions were freeze-dried to give the title compound as a solid (51.87mg, 73% Yield).
(ES+) 471
[00305] Compounds 1-42 to 1-81 were prepared using Method A, Steps 1-3 followed by
Method I-B, Steps 1-2.
Compound 1-43 6-(4-(l ,4-diazepane-1-carbonyl)phenyl)-3-amino-N-phenylpyrazine-2- carboxamide
1H NMR (400.0 MHz, DMSO) 1.44 - 1.47 (m, 1H), 1.53 - 1.58 (m, I H), 2.57 - 2.61 (m, I H),
2.62 - 2.69 (m, 2H), 2.74 - 2.80 (m, 1H), 3.15 - 3.20 (m, 2H), 3.40 - 3.46 (m, 2H), 6.91 - 6.96
(m, 1H), 7.15 - 7.19 (m, 2H), 7.23 - 7.28 (m, 2H), 7.51 (br s, 2H), 7.58 - 7.60 (m, 2H), 8.05 -
8.08 (m, 2H), 8.74 (s, 1H) and 10.20 (s, 1H) ppm; MS (ES+) 417 Compound 1-44 3-amino-6-(4-(3-methoxyazetidine-1-carbonyl)phenyl)-N-phenylpyrazine-
2-carboxamide
I H NMR (400 MHz, DMSO) 3.22 (3H, s), 3.87 (1H, br dd), 4.18 (1H, br d), 4.23-4.29 (2H, br dd), 4.47-4.49 (1 H, m), 7.17 ( 1 H, t), 7.40 (2H, t), 7.75 (2H, d), 7.79 (2H, br s), 7.83 (2H, d), 8.29 (2H, d), 9.00 (1H, s), 10.44 (1H, s) ppm; MS (ES+) 404
Compound 1-45 3-amino-6-(4-((2-methoxyethyl)(methyl)carbamoyl)phenyl)-N- phenylpyrazine-2-carboxamide
1H NMR (400 MHz, DMSO) 3.00 (3H, br s), 3.45 (3H, br s), 3.61 (2H, br d), 7.17 (1H, t),
7.41 (2H, t), 7.49 (2H, d), 7.76 (2H, br s), 7.84 (2H, d), 8.29 (1H, d), 8.97 (1H, s), 10.44 (1H, s) ppm; MS (ES+) 406
Compound 1-46 3-amino-N-phenyl-6-(4-(2-(pyrrolidin-1-yl) ethylcarbamoyl)phenyl) pyrazine-2-carboxamide
I H NMR (400 MHz, DMSO) 1.80 (4H, br s), 3.51 (2H, br s), 7.18 (1H, t), 7.41 (2H, t), 7.81-
7.85 (4H, m), 7.95 (2H, d), 8.35 (2H, d), 8.65 (1H, br s), 9.02 (1H, s), 10.44 (I H, s) ppm; MS
(ES+) 431
Compound 1-47 3-amino-N-phenyl-6-(4-(tetrahydro-2H-pyran-4-ylcarbamoyl) phenyl)pyrazine-2-carboxamide
1 H NMR (400 MHz, DMSO) 1.56-1.67 (2H, m), 1.75-1.80 (2H, m), 3.29-3.44 (2H, m), 3.88-
3.92 (2H, m), 4.00-4.07 (I H, m), 7.15 (I H, t), 7.41 (2H, t), 7.79 (2H, br s), 7.82 (2H, d), 7.97
(2H, d), 8.33 (2H, d), 8.40 (1H, d), 9.01 (1H, s), 10.44 (1H, s) ppm; MS (ES+) 418
Compound 1-48 3-amino-6-(3-(l-methylpiperidin-4-ylcarbarnoyl)phenyl)-N- phenylpyrazine-2-carboxamide
1H NMR (400 MHz, DMSO) 1.55-1.64 (2H, m), 1.76-1.81 (2H, m), 1.93 (2H, t), 2.16 (3H, s), 2.75 (2H, br d), 3.72-3.76 (1H, m), 7.12 (1H, t), 7.36 (2H, t), 7.54 (1H, t), 7.72 (2H, br s),
7.78-7.83 (3H, m), 8.37 (2H, dd), 8.55 (1H, s), 8.98 (I H, s), 10.44 (1H, s) ppm; MS (ES+)
431
Compound 1-49 3-amino-N-phenyl-6-(4-(2-(piperidin-1-yl) ethylcarbamoyl)phenyl) pyrazine-2-carboxamide
1H NMR (400 MHz, DMSO) 1.30-1.40 (2H, m), 1.46-1.53 (4H, m), 2.33 (4H, m), 2.45 (2H, t), 3.37-3.44 (2H, m), 7.16 (I H, t), 7.41 (2H, t), 7.79 (2H, br s), 7.81 (2H, d), 7.95 (2H. d),
8.34 (2H, d), 8.48 (1 H, t), 9.00 (I H, s), 10.45 (1 H, s) ppm; MS (ES+) 445 Compound 1-50 3-amino-6-(3-(4-(hydroxymethyl)piperidine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide
1H NMR (400 MHz, DMSO) 1.10-1.22 (3H, m), 1.65 (2H, br s), 1.79 (1H, br d), 2.77 (1H, br t), 3.05 (1H, br t), 3.27 (2H, d), 3.64 (1H, br d), 4.52 (1H, br s), 7.17 (1H, t), 7.38-7.42
(3H, m), 7.55 (1H, t), 7.73 (2H, brs), 7.80 (2H, d), 8.19 (I H, s), 8.29 (1H, d), 8.96 (1H, s),
10.45 (I H, s) ppm; MS (ES+) 432
Compound 1-51 3-amino-6-(4-(cycIopropylcarbamoyl)phenyl)-N-phenylpyrazine-2- carboxamide
1H ΝMR (400 MHz, DMSO) 0.59-0.67 (2H, m), 0.69-0.74 (2H, m), 2.84-2.91 (I H, m), 7.17
(I H, t), 7.21 (2H5 1), 7.79 (2H, br s), 7.81 (2H, d), 7.95 (2H, d), 8.39 (2H, d), 8.53 (I H, d),
8.97 1 H, s), 10.46 ( 1 H, s) ppm; MS (ES+) 374
Compound 1-52 3-amino-6-(3-((2-(dimethylamino)ethyl)(methyl)carbamoyl) phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 419
Compound 1-53 3-amino-N-phenyl-6-(3-(piperazine-1-carbonyl)phenyl) pyrazine-2- carboxamide; MS (ES+) 403
Compound 1-54 3-amino-N-phenyl-6-(3-(2-(pyrrolidin-1-yl)ethylcarbamoyl) phenyl)pyrazine-2-carboxamide; MS (ES+) 431
Compound 1-55 3-amino-6-(3-(3-(dimethylamino)pyrrolidine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 431
Compound 1-56 3-amino-N-phenyl-6-(3-(4-(pyrrolidin-1-yl)piperidine-1-carbonyl) phenyl)pyrazine-2-carboxamide; MS (ES+) 471
Compound 1-57 3-amino-6-(3-(4-hydroxycyclohexylcarbamoyl)phenyl)-N-phenylpyrazine -
2-carboxamide; MS (ES+) 432
Compound 1-58 3-amino-6-(3-(4-(2-cyanoethyl)piperazine-l -carbonyl)pheny I)-N- phenylpyrazine-2-carboxamide; MS (ES+) 456
Compound 1-59 3-amino-6-(3-(4-methylpiperazine-1-carbonyl)phenyI)-N-phenylpyrazine-2- carboxamide; MS (ES+) 417
Compound 1-60 3-amino-6-(3-(3-methoxyazetidine-1-carbonyl)phenyl)-N-phenylpyrazine -
2-carboxamide; MS (ES+) 404
Compound 1-61 3-amino-N-phenyl-6-(3-(2-(piperidin-1-yl)ethylcarbamoyl)phenyl) pyrazine-2-carboxamide; MS (ES+) 445 Compound 1-62 3-amino-6-(3-(4-carbamoylpiperidine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 445
Compound 1-63 3-amino-N-phenyl-6-(3-(pyrrolidine-1-carbonyl)phenyl)pyrazine-2- carboxamide; MS (ES+) 388
Compound 1-64 3-amino-6-(4-(l-methylpiperidin-4-ylcarbamoyl)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 431
Compound 1-65 3-amino-6-(3-(3-hydroxypyrrolidine-1-carbonyl)phenyl)-N-phenylpyrazine-
2-carboxamide; MS (ES+) 404
Compound 1-66 3-amino-N-phenyl-6-(3-(tetrahydro-2H-pyran-4-ylcarbamoyl)phenyl) pyrazine-2-carboxamide; MS (ES+) 418
Compound 1-67 3-amino-6-(3-((2-methoxyethyl)(methyl)carbamoyl)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 406
Compound 1-68 3-amino-6-(4-((2-(dimethylamino)ethyl)(methyl)carbamoyl) phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 419
Compound 1-69 3-amino-N-phenyl-6-(4-((tetrahydro-2//-pyran-4-yl)methylcarbamoyl) phenyl)pyrazine-2-carboxamide; MS (ES+) 432
Compound 1-70 3-amino-N-phenyI-6-(4-(pyrrolidine-1-carbonyl)phenyl)pyrazine-2- carboxamide; MS (ES+) 388
Compound 1-71 3-amino-N-phenyl-6-(4-(4-(pyrrolidin-1-yl)piperidine-1-carbonyl)phenyl) pyrazine-2-carboxamide; MS (ES+) 471
Compound 1-72 3-amino-6-(4-(azetidine-1-carbonyl)phenyl)-N-phenylpyrazine-2- carboxamide; MS (ES+) 374
Compound 1-73 3-amino-6-(4-(4-methylpiperazine-l -carbonyl)phenyl)-N-phenylpyrazine-2- carboxamide; MS (ES+) 417
Compound 1-74 3-amino-N-phenyl-6-(4-(piperazine-1-carbonyl)phenyl)pyrazine-2- carboxamide; MS (ES+) 403
Compound 1-75 3-amino-6-(4-(3-hydroxypyrrolidine-1-carbonyl)phenyl)-N-phenylpyrazine-
2-carboxamide; MS (ES+) 404
Compound 1-76 3-amino-6-(4-(3-(dimethylamino)pyrrolidine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 431 Compound 1-77 3-amino-6-(4-(4-carbamoylpiperidine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 445
Compound 1-78 3-amino-N-phenyl-6-(4-(piperidine-1-carbonyl)phenyl)pyrazine-2- carboxamide; MS (ES+) 402
Compound 1-79 3-amino-6-(4-(4-(hydroxymethyl)piperidine-1-carbony!)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 432
Compound 1-80 3-amino-6-(4-(4-(dimethylamino)piperidine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 445
Compound 1-81 3-amino-6-(4-(4-(2-cyanoethyl)piperazine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide; MS (ES+) 456
Example 3 : 3-amino-6-(4-(methylsulfonyl)phenyl)-Ν-phenylpyrazine-2-carboxatτiide
(Compound 1-82)
Figure imgf000176_0001
SCHEME C
Figure imgf000176_0002
[00306] Compound 1-82 was prepared using Method A, Step 1 followed by Method I-C, Steps 1-3
METHOD I C
Step 1 : Methyl 3-amino-6-(4-(methylsulfonyl)phenyl)pyrazine-2-carboxylate
Figure imgf000177_0001
[00307] A mixture of methyl 3-amino-6-bromo-pyrazine-2-carboxylate (1.5 g, 6.465 mmol), (4-methylsulfonylphenyl)boronic acid (1.552g, 7.758 mmol), bis(triphenylphosphine) palladium(II)dichloride (226.9 mg, 0.3233 mmol), and Na2CO3 (9.700 mL of 2 M, 19.40 mmol) in DME (18.75 mL) were heated in the microwave at 110°C for 1 hour. The resultant mixture was diluted with EtOAc and washed with water. The organic phase was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified on silica gel by flash column chromatography (50% EtOAc / hexanes) to afford the title compound (600mg, 53% Yield).
[00308] 1 H NMR (400 MHz, DMSO) 3.25 (3H, s), 3.92 (3H, s), 7.61 (2H, br s), 8.00 (2H, m), 8.26 (2H, m), 9.03 (H, s) ppm; MS (ES+) 308
Step 2: 3-amino-6-(4-(methylsulfonyl)phenyl)pyrazine-2-carboxylic acid
Figure imgf000177_0002
[00309] A mixture of methyl 3-amino-6-(4-(methylsulfonyl)phenyl)pyrazine-2- carboxylate (3.50 g, 11.39 mmol) and LiOH (1.364g, 56.95 mmol) was dissolved in Methanol (14 mL) and water (14ml) and allowed to heat at 90°C for 2 hours. The reaction mixture was allowed to cool and neutralized with IM HCl. The resultant precipitate was collected by filtration to afford the pure product as a yellow solid (3.32g, 99% Yield). MS (ES+) 293
Step 3: 3-amino-6-(4-(methylsulfonyl)phenyl)-N-phenylpyrazine-2-carboxamide (Compound 1-82)
Figure imgf000178_0001
[00310] A mixture of 3-amino-6-(4-methylsulfonylphenyl)pyrazine-2-carboxylic acid (1.5 g, 5.1 14 mmol), diethoxyphosphorylformonitrile (926.8 mg, 849.5 μL, 5.1 14 mmol), aniline (476.2 mg, 465.9 μL, 5.1 14 mmol) and triethylamine (1.035 g, 1.426 mL, 10.23 mmol) were stirred in DME (18.75 mL) at 120 °C for 18 hours. After this time water was added and the resultant solid collected by filtration. The solid was triturated with acetone and dried to give the desired product (1.335g, 71% Yield). 1H NMR (400.0 MHz, DMSO) d 3.28 (s, 3H), 7.18 (t, J = 7.3 Hz, I H), 7.41 (t, J = 7.8 Hz, 2H), 7.82 (d, J = 7.9 Hz, 2H), 7.89 (s, 2H), 8.01 (d, J = 8.4 Hz, 2H), 8.51 (d, J = 8.4 Hz, 2H), 9.04 (s, 1H) andlθ.47 (s, I H) ppm; MS (ES+) 369
[00311] Compounds 1-82 to 1-108 were all prepared using Method A, Step 1 followed by Method I-C, Steps 1-3.
Compound 1-82 3-amino-6-(4-(methylsulfonyl)phenyl)-N-phenylpyrazine-2-carboxamide
1H NMR (400.0 MHz, DMSO) d 3.28 (s, 3H), 7.18 (t, J = 7.4 Hz, I H), 7.43 - 7.39 (m, 2H),
7.83 - 7.81 (m, 2H), 7.89 (s, 2H), 8.01 (dd, J = 1.6, 7.0 Hz, 2H), 8.51 (d, J = 8.5 Hz, 2H),
9.04 (s, 1H) and 10.46 (s, 1H) ppm; MS (ES+) 369.
Compound 1-83 3-amino-N-(1H-indol-7-yl)-6-(pyridin-3-yl)pyrazine-2-carboxamide
1H NMR (400.0 MHz, DMSO) d 6.50 (dd, J = 2.0, 2.9 Hz, 1H), 7.04 (t, J = 7.7 Hz, 1H), 7.21
(d, J = 7.4 Hz, 1H), 7.35 (t, J = 2.8 Hz, 1H), 7.50 (d, J = 7.8 Hz, 1H), 7.79 (dd, J = 5.2, 8.0 Hz, 3H), 8.73 (dd, J = 1.2, 5.2 Hz, 1H), 9.03 (d, J = 8.2 Hz, I H), 9.09 (s, 1H), 9.65 (d, J = 1.9
Hz, 1 H), 10.67 (s, 1 H) and 1 1.00 (s, 1 H) ppm; MS (ES+) 331
Compound 1-84 3-amino-N-(4-methoxyphenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide
1H NMR (400.0 MHz, DMSO) d 3.76 (s, 3H), 6.98 (dd, J = 2.1 , 6.9 Hz, 2H), 7.69 (dd, J =
2.1, 6.9 Hz, 2H), 7.84 (dd, J = 5.2, 8.1 Hz, 3H), 8.76 (dd, J = 1.2, 5.2 Hz, 1H), 9.01 - 9.06 (m,
2H), 9.62 (d, J = 1.9 Hz, 1H) and 10.46 (s, 1H) ppm; MS (ES+) 322
Compound 1-85 3-amino-N-phenyl-6-(pyridin-3-yl)pyrazine-2-carboxamide
I H NMR (400.0 MHz, DMSO) d 7.17 (t, 1H), 7.49 (t, 2H), 7.68 (t, 1H), 7.82 (d, 2H), 7.87
(br s, 2H), 8.68 (d, 1H), 8.81 (d, I H), 9.12 (s, 1H), 9.51 (s, 1H) and 10.48 (s, 1H) ppm; MS
(ES+) 292
Compound 1-86 3-amino-N-(3-methoxyphenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide
I H NMR (400 MHz, DMSO) d 3.79 (3H, s), 6.74 (I H, dd), 7.30 (I H, t), 7.44 (1H, d), 7.50-
7.52 (2H, m), 7.8 (2H, br s), 8.59-8.62 (2H, m), 9.00 (I H, s), 9.44 (1H, s) and 10.42 (1H, s) ppm; MS (ES+) 322
Compound 1-87 3-amino-N-(3-cyanophenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide; MS
(ES+) 317
Compound 1-88 3-amino-N-(3-carbamoylphenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide;
MS (ES+) 335
Compound 1-89 3-amino-6-(pyridin-3-yl)-N-(pyrimidin-4-yl)pyrazine-2-carboxamide; MS
(ES+) 294
Compound 1-90 3-amino-N-(3-(dimethylamino)phenyl)-6-(pyridin-3-yl)pyrazine-2- carboxamide; MS (ES+) 335
Compound 1-91 3-amino-6-(pyridin-3-yl)-N-o-tolylpyrazine-2-carboxarnide
MS (ES+) 306
Compound 1-92 3-amino-N-(4-carbamoylphenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide;
MS (ES+) 335
Compound 1-93 3-amino-N-(4-ethanamidophenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide;
MS (ES+) 349
Compound 1-94 3-amino-N-(4-fluorophenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide; MS
(ES+) 310 Compound 1-95 3-amino-N-(3-ethanamidophenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide;
MS (ES+) 349
Compound 1-96 3-amino-N-(2-fluorophenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide; MS
(ES+) 310
Compound 1-97 3-amino-N-(pyridin-2-yI)-6-(pyridin-3-yl)pyrazine-2-carboxamide; MS
(ES+) 293
Compound 1-98 3-amino-6-(pyridin-3-yl)-N-(pyridin-4-yl)pyrazine-2-carboxamide; MS
(ES+) 293
Compound 1-99 3-amino-N-(2,2-difluorobenzo[ύT|[1,3]dioxol-4-yl)-6-(pyridin-3- yl)pyrazine-2-carboxamide; MS (ES+) 372
Compound 1-100 3-amino-N-(5-ethanamido-2-methoxyphenyl)-6-(pyridin-3-yl)pyrazine-2- carboxamide; MS (ES+) 379
Compound 1-101 3-amino-6-(pyridin-3-yl)-N-(3-sulfamoylphenyl)pyrazine-2-carboxamide;
MS (ES+) 371
Compound 1-102 3-amino-6-(pyridin-3-yl)-N-(2-(trifluoromethoxy)phenyl)pyrazine-2- carboxamide; MS (ES+) 376
Compound 1-103 3-amino-N-(3-fluorophenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide; MS
(ES+) 310
Compound 1-104 3-amino-N-(1H-indol-5-yl)-6-(pyridin-3-yl)pyrazine-2-carboxamide; MS
(ES+) 331
Compound 1-105 3-amino-N-(lH-indol-6-yl)-6-(pyridin-3-yl)pyrazine-2-carboxamide; MS
(ES+) 331
Compound 1-106 3-amino-N-(2-methoxyphenyl)-6-(pyridin-3-yl)pyrazine-2-carboxamide;
MS (ES+) 322
Compound 1-107 3-amino-N-(2,5-dimethoxyphenyl)-6-(pyridin-3-yl)pyrazine-2- carboxamide; MS (ES+) 352
Compound 1-108 3-amino-N-(2-methoxy-5-methylphenyl)-6-(pyridin-3-yl)pyrazine-2- carboxamide; MS (ES+) 336 Example 4: 2-(3-amino-6-(4-(methylsulfonyl)phenyl)pyrazin-2-viyi H-benzordlimidazol-7- ol (Compound 1-109)
Figure imgf000181_0001
SCHEME D
Figure imgf000181_0002
[00312] Compound 1-109 was prepared using Method A, Step 1 followed by Method I-C, Steps 1-2 followed by Method I-D, Step 1.
METHOD I-D
Step 1: 2-(3-amino-6-(4-(methylsulfonyl)phenyl)pyrazin-2-yl)-1H-benzo[d]imidazol-7-ol
Figure imgf000181_0003
[00313] A mixture of 3-amino-6-(4-methylsulfonylphenyl)pyrazine-2-carboxylic acid (120 mg, 0.4091 mmol) , diethoxyphosphorylformonitrile (73.40 mg, 0.4500 mmol), triethylamine (124.2 mg, 171.1 μL, 1.227 mmol) and 2,3-diaminophenol (50.79 mg, 0.4091 mmol) in DME (5 mL) was heated in the microwave at 170°C for lhour. The mixture was diluted with EtOAc, washed with water and brine and concentrated in vacuo The residue was then dissolved in DCM and triturated with ether to give the desired product as a yellow solid (115mg, 74% Yield). 1H NMR (400 MHz, DMSO) 3.6 (3H, s), 6.65 (1H, d), 7.1-7.18 (2H, m), 8.0-8.1 (4H, m), 8.6 (2H, d), 8.9 (1H, s), 9.05(1 H, br s), 9.9 (1H, s), 12.9 (1H, b rs) ppm; MS (ES+) 382
[00314] Compounds 1-109 to 1-121 were prepared using Method A, Step 1 followed by Method I-C, Steps 1-2 followed by Method I-D, Step 1.
Compound 1-110 3-(1H-benzo[d]imidazol-2-yl)-5-phenylpyrazin-2-amine
I H NMR (400 MHz, CDCl3) 1.5 (2H, br s), 7.35-7.7 (3H, m), 7.5-7.67 (3H, m), 7.87 (I H, d),
8.02 (1H, d), 8.62 (I H, s), 10.45 (I H, s) ppm; MS (ES+) 288
Compound 1-111 2-(3-amino-6-(4-(methylsulfonyl)phenyl)pyrazin-2-yl)-1H-benzo[d] imidazole-6-carbonitrile
1H NMR (400 MHz, DMSO) 3.3 (3H, s), 7.7-7.85 (2H, m), 8.05 (2H, d), 8.43 (1H, s),
8.55(2H, d), 9.05 (1H, s), 13.55 (I H, br s) ppm; MS (ES+) 389
Compound 1-112 3-(3H-imidazo[4,5-ό]pyridin-2-yl)-5-(4-(methylsulfonyl)phenyl) pyrazin-
2-amine
1H NMR (400 MHz, CDCl3) 3.05-3.1 (3H, m), 7.4-7.5 (2H, m), 7.95-8.05 (2H, m), 8.3-8.42
(3H, m), 8.8 ( 1 H, m) ppm; MS (ES+) 367
Compound 1-113 2-(3-amino-6-phenylpyrazin-2-yl)-1H-benzo[d]imidazol-7-ol
1H NMR (400 MHz, DMSO) 6.63 (I H, d), 7.05-7.15 (2H, m), 7.4-7.44 (1H, m), 7.5-7.53
(3H, m), 8.3 (I H, d), 8.75 (2H, s), 9.95 (1H, s), 12.9 (1H, s) ppm; MS (ES+) 304
Compound 1-114 3-(6-chloro-l H-benzo[d]imidazol-2-yl)-5-(4-(methylsulfonyl)phenyl) pyrazin-2-amine
1H NMR (400 MHz, DMSO) 3.35 (3H, s), 7.25-7.35 (1H, m), 7.58-7.62 (I H, m), 7.75-7.85
(1H, m), 7.95-8.0 (2H, m), 8.45-8.52 (2H, m), 8.65-8.8 (1H, br s), 8.92-8.94 (1H, m), 13.2-
13.26 (1H, m) ppm; MS (ES+) 400
Compound 1-115 3-(6-methoxy-l H-benzo[d]imidazol-2-yl)-5-(4-(methylsulfonyl)phenyl) pyrazin-2-amine
1H NMR (400 MHz, DMSO) 3.3 (3H, s), 3.85 (3H, s), 6.9-6.93 (1H, m), 7.1-7.3 (I H, m),
7.6-7.7 (I H, m), 8.05 (2H, d), 8.6 (2H, d), 8.95 (1H, s), 13.1 (1H, br s) ppm; MS (ES+) 396 Compound 1-116 methyl 2-(3-amino-6-(4-(methy!sulfonyl)phenyl)pyrazin-2-yl)-1H- benzo[d]imidazole-6-carboxylate
1H NMR (400 MHz, DMSO) 3.2.8-3.32 (3H, m), 3.9-3.95 (3H,m), 7.7-7.75 (I H, m), 7.9-7.92 (1H, m), 8.0-8.1 (3H, m), 8.3 (0.5H, s), 8.42 (0.5H, s), 8.52-8.6 (2H, m), 8.7 (1H, br s), 9.0- 9.03 (l H,m), 13.4-13.48 (1H, m) ppm; MS (ES+) 424
Compound 1-117 3-(6-methyl-1H-benzo[d]imidazol-2-yl)-5-(4-(methylsulfonyl)phenyl) . pyrazin-2-amine; 1H NMR (400 MHz, DMSO) 2.5 (3H, s), 3.35 (3H, s), 7.05-7.1 (1H, m), 7.4-7.7 (I H, m), 8.03 (2H, d), 8.57 (1H, d), 8.95(1H, s), 12.95-13.05 (1H, m) ppm; MS (ES+) 380
Compound 1-118 5-(4-(methylsulfonyl)phenyl)-3-(6-(trifluoromethyl)-lH-benzo[ύ(] imidazol-2-yl)pyrazin-2-amine; 1Η NMR (400 MHz, DMSO) 3.3 (3H, s), 3.85 (3H, s), 6.9- 6.93 (1H, m), 7.1-7.3 (1H, m), 7.6-7.7 (1H, m), 8.05 (2H, d), 8.6 (2H, d), 8.95 (1H, s), 13.1 ( 1 H, br s) ppm; MS (ES+) 434
Compound 1-119 3-(7-methyl-l H-benzo[d]imidazol-2-yl)-5-(4-(methylsulfonyl)phenyl) pyrazin-2-amine; 1H NMR (400 MHz, DMSO) 2.6-2.7 (3H, m), 3.3 (3H, s), 7.1-7.25 (2H, m), 7.47 (I H, d), 8.0-8.1 (3H, m), 8.6 (1H, d), 8.95(1 H, s), 9.05 (1H, br s), 12.7 (0.2H, br s), 13.1 (1H, br s) ppm; MS (ES+) 380
Compound 1-120 3-(3H-imidazo[4,5-c]pyridin-2-yl)-5-(4-(methylsulfonyl)phenyl) pyrazin- 2-amine; 1 Η NMR (400 MHz, DMSO) 3.13 (3H, s), 7.4-7.45 (1H, m), 7.5-7.6 (I H, m), 7.8- 7.85 (2H, m), 8.2-8.25 (1H, m), 8.35-8.4 (2H, m), 8.7-8.75(1H, m), 8.9 (1H, s), 13.25-13.35 (1H, m) ppm; MS (ES+) 367
Compound 1-121 3-(1H-benzo[<i]imidazol-2-yl)-5-(pyridin-3-yl)pyrazin-2-amine 1Η NMR (400 MHz, CDC13) 7.25-7.35 (3H, m), 7.35-7.4 (1H, m), 7.52 (1H, d), 7.78 (1H, d), 8.17 (1H, d), 8.55 (1H, s), 8.59-8.62 (1H, m), 9.17-9.19 (1H, m) ppm; MS (ES+) 289
Example 5 : 3-(lH-benzordlimidazol-2-vO-5-(3-(methylsulfonvQphenvOpyrazin-2-amine (-Compound 1-122)
Figure imgf000184_0001
SCHEME E
Figure imgf000184_0002
[00315] Compound 122 was prepared using Method A, Steps 1-2 followed by Method I-E, Steps 1-2.
METHOD I-E
Step 1 : 3-(l H-benzo[d]imidazol-2-yl)-5-bromopyrazin-2-amine
Figure imgf000184_0003
[00316] A mixture of 3-amino-6-bromo-pyrazine-2-carboxylic acid (1Og, 45.87 mmol), benzene- 1,2-diamine (5.45g, 50.46 mmol), diethoxyphosphorylformonitrile (8.23g, 50.46 mmol) and triethylamine (12.79 mL, 91.74 mmol) in DME (30 mL) was heated in the microwave at 170°C for 40 minutes. The mixture was allowed to cool and water was added. The resultant dark-coloured precipitate was dissolved in EtOAc and stirred with charcoal for 30 minutes. After filtering through celite, the filtrate was concentrated in vacuo to give the product as a yellow solid (8.04g, 60% Yield). IH NMR (400 MHz, DMSO) 7.22-7.32 (2H, m), 7.55 (IH, d), 7.75 (IH, d), 7.8 (Ih, br s), 8.8 (1H, br s), 13.1 (IH, s); MS (ES+) 291 Step 2 : 3-(l H-benzo[d]imidazol-2-yl)-5-(3-(methylsulfonyl)phenyl)pyrazin-2-amine
[00317] A mixture of 3-(1H-benzimidazol-2-yl)-5-bromo-pyrazin-2-amine(50 mg, 0.1723 mmol), 3-methylsulfonylphenyl)boronic acid (34.46mg, 0.1723 mmol), dichloropalladium; triphenylphosphane (6.047 mg, 0.008615 mmol) and disodium carbonate (258.5 μL of 2 M, 0.5169 mmol) in DME (625.0 μL) was heated in the microwave at 110°C for lhour and then at 150°C for 3 hours. The mixture was diluted with EtOAc and washed with water. The organic layer was separated, dried (MgSC^) and concentrated in vacuo. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, lOuM, IOOA column, gradient 10% - 95%B (solvent A: 0.05% TFA in water, solvent B: CH3CN) over 16 minutes at 25mL/min]. The fractions were freeze-dried to give the title compound as a solid (37.7 mg, 60% Yield). 1H NMR (400 MHz, CDCl3) 3.2 (3H, s), 7.3-7.45 (2H, m), 7.65 (1H, d), 7.75 (1H, t), 7.85 (I H, d), 8.0 (I H, d), 8.23 (1H, d), 8.65 (2H, s), 10.55 (1H, s); MS (ES+) 366 [00318] Compounds 1-122 to 1-137 were prepared was prepared using Method A, Steps 1- 2 followed by Method I-E, Steps 1 -2.
Compound 1-123 3-(l H-benzo[d]imidazol-2-yl)-5-(4-(methylsulfonyl) phenyl)pyrazin-2- amine
1H NMR (400 MHz, DMSO) 3.4 (3H, s), 5.75 (2H, s), 7.2-7.38 (2H, m), 7.65 (1H, d), 7.8
(1H, d), 8.05 (1H, d), 8.55 (1H, d), 8.95 (2H, s), 13.3 (1H, s) ppm; MS (ES+) 366
Compound 1-124 4-(5-amino-6-( 1 H-benzo[d]imidazol-2-yl)pyrazin-2-yl)-N,N- dimethylbenzamide
1H NMR (400 MHz, DMSO) d 2.99 (s, 3H), 3.02 (s, 3H), 7.31 (dd, J = 3.0, 6.0 Hz, 2H), 7.54
(d, J = 8.4 Hz, 2H), 7.72 (s, 2H), 8.35 (d, J = 8.4 Hz, 2H) and 8.86 (s, I H) ppm; MS (ES+)
359
Compound 1-125 (3-(5-amino-6-(l H-benzo[d]imidazol-2-yl)pyrazin-2-yl)phenyl)
(morpholino)methanone; MS (ES+) 401
Compound 1-126 3-(5-amino-6-(l H-benzo[d]imidazol-2-yl)pyrazin-2-yl)phenol
MS (ES+) 304
Compound 1-127 (2-(5-amino-6-(l H-benzo[d]imidazol-2-yl)pyrazin-2-yl)phenyl)methanol
1H NMR (400.0 MHz, DMSO) d 4.72 (s, 2H), 7.27 (q, J = 3.0 Hz, 2H), 7.38 - 7.47 (m, 2H),
7.55 - 7.67 (m, 5H) and 8.37 (s, I H) ppm; MS (ES+) 318 Compound 1-128 4-(5-amino-6-(1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)-N-(3- hydroxypropyl)benzamide; MS (ES+) 389
Compound 1-129 4-(5-amino-6-(1H-benzo[d]imidazol-2-yl)pyrazin-2-yI)benzonitrile; MS
(ES+) 313
Compound 1-130 N-(4-(5-amino-6-(l H-benzo[d]imidazol-2-yl)pyrazin-2-yl) benzyl) ethanamide; MS (ES+) 359
Compound 1-131 (5-(5-amino-6-(1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)-2-fluorophenyl)
(morpholino)methanone; MS (ES+) 419
Compound 1-132 4-(5-amino-6-(l H-benzo[d]imidazol-2-yl)pyrazin-2-yl)-N-(2- hydroxyethyl)benzamide; MS (ES+) 375
Compound 1-133 4-(5-amino-6-(1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)-N-(2-(pyrrolidin- l-yl)ethyl)benzamide; MS (ES+) 428
Compound 1-134 3-(1H-benzo[d]imidazol-2-yl)-5-(4-(pyrrolidin-1-ylsulfonyl)phenyl) pyrazin-2-amine; MS (ES+) 421
Compound 1-135 3-(l//-benzo[</]imidazol-2-yl)-5-(6-morpholinopyridin-3-yl)pyrazin-2- amine; 1H NMR (400.0 MHz, DMSO) d 3.57 - 3.59 (m, 4H), 3.75 - 3.77 (m, 4H), 7.07 (d, J
= 9.1 Hz, I H), 7.28 - 7.32 (m, 2H), 7.71 (s, 2H), 8.53 (d, J = 8.2 Hz, 1H), 8.77 (s, 1H) and
9.03 (d, J = 2.0 Hz, 1H) ppm; MS (ES+) 374
Compound 1-136 3-(lH-benzo[</|imidazoI-2-yl)-5-(2-(piperazin-1-yl)pyridin-4-yl)pyrazin-
2-amine; MS (ES+) 373
Compound 1-137 5-(5-amino-6-(lH-benzo[</|imidazol-2-yl)pyrazin-2-yl)pyridin-2-ol; MS
(ES+) 305
Example 6 : 3-(5-phenyl-4Η-l ,2,4-triazol-3-vD-5-(pyridin-3-yl)pyrazin-2-amine (Compound
1-138)
Figure imgf000186_0001
SCHEME F
od I-F 4
Figure imgf000187_0001
Compound 138 was prepared using Method A, Step 1 followed by Method I-F, Steps 1 -4.
METHOD I-F
Step 1 : 3-amino-6-(pyridin-3-yl)pyrazine-2-carboxylic acid
Figure imgf000187_0002
[00319] A mixture of methyl 3-amino-6-bromo-pyrazine-2-carboxylate (8 g, 34.48 mmol) , diethyl-(3-pyridyl)borane (6.084 g, 41.38 mmol) , dichloropalladium; triphenylphosphane (1.210 g, 1.724 mmol) and disodium carbonate (51.70 mL of 2 M, 103.4 mmol) in DME (100 mL) were heated overnight at 80 °C. The reaction mixture was cooled and EtOAc was added. The resultant precipitate was collected, treated with water and the resultant suspension heated and filtered hot. The solution was then allowed to cool and acidified with AcOH to approx pH 5. The precipitate was collected, washed with MeOH and dried to yield the product as a yellow powder (6.22 g, 83 % Yield). 1H NMR (400.0 MHz, DMSO) d 7.49 (dd, J = 4.8, 7.4 Hz, I H), 7.60 (s, 2H), 8.44 (d, J = 7.6 Hz, I H), 8.57 (d, J = 3.7 Hz, 1H), 8.97 (s, 1 H) and 9.27 (s, 1 H) ppm; MS (ES+) 217 Step 2: Methyl 3-amino-6-(pyridin-3-yl)pyrazine-2-carboxylate
Figure imgf000188_0001
[00320] To 3-amino-6-(3-pyridyl)pyrazine-2-carboxylic acid (2 g, 9.251 mmol) in MeOH (50 mL) was added cone. H2SO4 (907.3 mg, 493.1 μL, 9.251 mmol) and the mixture heated to reflux for 2 hours. The solvent was removed in vacuo and the mixture neutralised with aq. Na2CO3. The resulting brown solid was collected by filtration and dried (2.08 g, 97% Yield). MS (ES+) 231
Step 3: 3-amino-6-(pyridin-3-yl)pyrazine-2-carbohydrazide
Figure imgf000188_0002
[00321] A mixture of methyl 3-amino-6-(3-pyridyl)pyrazine-2-carboxylate (2 g, 8.687 mmol) was heated in Hydrazine (1.392 g, 1.363 mL, 43.43 mmol) with a minimal amount of MeOH (5 mL) added at 80 °C for 2 hours. Water was added and the product collected by filtration, washed with methanol and dried to furnish the product as a brown solid (1.17 g, 58 % Yield). I H NMR (400.0 MHz, DMSO) d 4.52 (br s, 2H), 7.43 (m, I H), 7.71 (s, 2H), 8.54 (2H, m), 8.90 (1H, s), 9.39 (1H, s), 10.16 (1H, s) ppm; MS (ES+) 231
Step 4: 3-(5-phenyl-4H-1,2,4-triazol-3-yl)-5-(pyridin-3-yl)pyrazin-2-amine (Compound 138)
Figure imgf000188_0003
[00322] A mixture of 3-amino-6-(3-pyridyl)pyrazine-2-carbohydrazide (40 mg, 0.173 mmol) , benzamidine hydrochloride (27.2 mg, 0.173 mmol) and sodium ethanolate (1 1.82 mg, 0.173 mmol) were added to a 5 mL microwave vial in DMF (1 mL). The reaction mixture was heated in the microwave at 200 °C for 20 min. The mixture was concentrated in vacuo and the residue purified by reverse phase preparative HPLC [Waters Sunfire C 18, lOuM, IOOA column, gradient 10% - 95%B (solvent A: 0.05% TFA in water, solvent B: CFhCN) over 16 minutes at 25mL/min]. The fractions were freeze-dried to give the title compound as a solid (12.5 mg, 20% Yield). 1H NMR (500 MHz, DMSO) d 7.5 (m, 3H), 7.66 (m, 1H), 7.94 (br s, 2H), 8.16 (m, 2H), 8.66 (s, 1H), 8.79 (br s, 1H), 8.96 (s, 1H), 9.52 (s, 1H) and 14.94 (s, I H) ppm; MS (ES+) 316
[00323] Compounds 1-138 to 1-143 were prepared using Method A, Step 1 followed by Method I-F, Steps 1-4
Compound 1-139 : 3-(5-(4-(aminomethyl)phenyl)-4H-1,2,4-triazol-3-yl)-5-(pyridin-3- yl)pyrazin-2-amine; MS (ES+) 345
Compound 1-140 3-(5-(3-aminophenyl)-4//-l ,2,4-triazol-3-yl)-5-(pyridin-3-yl)pyrazin-2- amine; 1 H NMR (400.0 MHz, DMSO) d 6.98 - 7.03 (m, 1 H), 7.39 (t, J = 7.8 Hz, 1 H), 7.74 (s, 2H), 7.82 (dd, J = 5.2, 8.1 Hz, I H), 8.06 (s, 2H), 8.74 (dd, J = 1.3, 5.2 Hz, 1H), 8.96 (d, J = 7.9 Hz, 1 H), 9.02 (s, 1 H), 9.60 (s, 1 H) and 15.03 (br s, 1 H) ppm; MS (ES+) 331
Compound 1-141 5-(pyridin-3-yl)-3-(5-m-tolyl-4H-1,2,4-triazol-3-yl)pyrazin-2-amine; MS (ES+) 330
Compound 1-142 5-(pyridin-3-yl)-3-(5-(thiophen-2-yl)-4Η-1,2,4-triazol-3-yl)pyrazin-2- amine ; 1H NMR (400.0 MHz, DMSO) d 7.22 (dd, J = 3.8, 4.8 Hz, 1H), 7.68 - 7.73 (m, 2H), 7.81 (d, J = 3.0 Hz, I H), 7.95 (s, 2H), 8.69 (dd, J = 1.2, 4.9 Hz, 1H), 8.84 (d, J = 6.1 Hz, I H), 8.99 (s, 1H), 9.55 (s, 1H) and 14.96 (s, 1H) ppm; MS (ES+) 322
[00324] Compound 1-143 3-(5-(3-(aminomethyl)phenyl)-4H-l ,2,4-triazol-3-yl)-5- (pyridin-3-yl)pyrazin-2-amine; MS (ES+) 345 Example 7 : 5-(4-(methylsulfonyl)phenyl)-3-(5-phenyl-1,2,4-oxadiazol-3-yl)pyrazin-2-amine (Compound 1-144) [there are some compounds from the PRV that are repeated with new number in the table! Like this one!!
Figure imgf000190_0001
SCHEME G
Figure imgf000190_0002
METHOD I-G
Step 1: 3-amino-6-bromo-N'-hydroxypyrazine-2-carboximidamide
Figure imgf000190_0003
[00325] A solution of 3-amino-6-bromo-pyrazine-2-carbonitrile (1 g, 5.025 mmol) in MeOH (20 mL) was cooled to O °C and treated with hydroxylamine hydrochloride (349.2 mg, 5.025 mmol) and triethylamine (508.5 mg, 700.4 μL, 5.025 mmol) and the reaction allowed to warm to ambient temperature. After a period of 2 hours a precipitate was observed which was filtered off. The resultant filtrate was evaporated to dryness and triturated with MeOH to furnish further product as a beige solid (771 mg, 78% Yield). [00326] 1H NMR (400.0 MHz, DMSO) d 5.88 (s, 2H), 7.64 (br s, 2H), 8.14 (s, I H) and 10.38 (s, I H) ppm; MS (ES+) 233 Step 2: 3-amino-N'-(benzoyloxy)-6-brornopyrazine-2-carboximidamide
Figure imgf000191_0001
[00327] 3-amino-6-bromo-N'-hydroxypyrazine-2-carboximidamide (770 mg, 3.318 mmol) was suspended in DCM (10 mL) and triethylamine (369.3 mg, 508.7 μL, 3.650 mmol) followed by benzoyl chloride (513.1 mg, 423.7 μL, 3.650 mmol) were added. After 1 hour, the solvent was removed in vacuo and the residue triturated with MeOH. The resultant filtrate was filtered off to furnish the product as an off-white solid (779 mg, 70% Yield). [00328] 1H NMR (400.0 MHz, DMSO) d 7.18 (br s, 2H), 7.55 - 7.59 (m, 2H), 7.69 - 7.73 (m, 1H), 7.89 (br s, 2H), 8.28 - 8.30 (m, 2H) and 8.32 (s, 1H) ppm; MS (ES+) 337
Step 3: 5-bromo-3-(5-phenyl-1,2,4-oxadiazol-3-yl)pyrazin-2-amine
Figure imgf000191_0002
[00329] A mixture of 3-amino-N'-(benzoyloxy)-6-bromopyrazine-2-carboximidamide (575 mg, 1.711 mmol) and polyphosphonic acid (2.300 mL) was heated at 70 °C for 3.5 hours. The resultant solution was diluted with water (20 mL), quenched with NaHCO3 and the resultant product isolated by filtration (475 mg, 87% Yield) as a fawn solid. [00330] 1H NMR (400.0 MHz, DMSO) d 7.48 (br s, 2H), 7.67 - 7.71 (m, 2H), 7.76 - 7.78 (m, 1H), 8.26 - 8.28 (m, 2H) and 8.43 (s, 1H) ppm; MS (ES+) 319
Step 4: 5-(4-(methylsulfonyl)phenyl)-3-(5-phenyl-l ,2,4-oxadiazol-3-yl)pyrazin-2-amine (Compound 144)
Figure imgf000192_0001
[00331] A mixture of 5-bromo-3-(5-phenyl-l ,2,4-oxadiazol-3-yl)pyrazin-2-amine (100 mg, 0.3143 mmol), (4-methylsulfonylphenyl)boronic acid (94.29 mg, 0.4714 mmol) and
PdCl2(PPh3)2 (1 1.03 mg, 0.01572 mmol) in DMF (2 mL) was treated with Na2CO3 (471.4 μL of 2 M, 0.9429 mmol) and the reaction placed under an atmosphere of nitrogen and heated at
1 10 °C in a sealed tube for 16 hours. The resultant precipitate was filtered, washed with water and dried under vacuum (83 mg, 67% Yield).
[00332] 1H NMR (400.0 MHz, DMSO) d 3.27 (s, 3H), 7.58 (br s, 2H), 7.69 - 7.73 (m,
2H), 7.77 - 7.81 (m, 1H), 8.05 (d, J = 8.5 Hz, 2H), 8.32 (dd, J = 8.5, 18.0 Hz, 4H) and 9.04 (s,
1H) ppm; MS (ES+) 394
[00333] Compound IIA-1 was also prepared using Method I-G.
Compound HA-I: 3-(5-phenyl-l ,2,4-oxadiazol-3-yl)-5-(pyridin-3-yl)pyrazin-2-amine
Figure imgf000192_0002
1 H NMR (400.0 MHz, DMSO) d 7.32 (br s, 2H), 7.38 (dd, J = 4.3, 8.0 Hz, 1 H), 7.52 - 7.56 (m, 2H), 7.59 - 7.64 (m, I H), 8.12 - 8.14 (m, 2H), 8.24 - 8.27 (m, I H), 8.44 (dd, J = 1.6, 4.8 Hz, 1H), 8.82 (s, 1H) and 9.1 1 (d, J = 1.8 Hz, 1H) ppm; MS (ES+) 317
Example 8 : 3-(benzor^thiazol-2-yl)-5-(4-(methylsulfony0phenyl)pyrazin-2-arnine (Compound 1-146)
Figure imgf000193_0001
SCHEME H
Figure imgf000193_0002
[00334] Compound 1-146 was prepared using Method A, Steps 1 followed by Method I-C, Steps 1-2 followed by Method I-H, Step 1.
METHOD I-H
Step 1: 3-(benzo[d]thiazol-2-yl)-5-(4-(methylsulfonyl)phenyl)pyrazin-2-amine (Compound
1-146)
Figure imgf000193_0003
[00335] 3-amino-6-(4-methylsulfonylphenyl)pyrazine-2-carboxylic acid (350 mg, 1.193 mmol), was heated in thionyl chloride (4.258 g, 2.61 1 mL, 35.79 mmol) at 70°C for lhour. The mixture was concentrated to dryness and washed several times with ether. The resultant acid chloride (150mg, 0.458 mmol) was dissolved in acetonitrile, treated with 2- aminobenzothiol (172mg, 1.374mmol) and heated at 7O°C for 2 hours. The mixture was diluted with EtOAc and washed with sat. aq. Na2CO3, water and brine. The organic phase was dried over magnesium sulfate, filtered and concentrated in vacuo. The residue was purified on silica gel by flash column chromatography (30 - 70% EtOAc / hexanes) to afford the title compound as a yellow solid after trituration with DCM/diethyl ether (102mg, 52% Yield); I H NMR (400 MHz, CDCl3) 3.3 (3H, s), 7.65-7.8 (2H, m), 8.2 (1H, d), 8.25 - 8.3 (3H, m), 8.45 (2H, d), 8.8 (1H, br s), 8.85 (1H, s) ppm; MS (ES+) 383
Example 9 : tert-butyl 4-(4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2- vPphenylcarbonyl)-1,4-diazepane-1-carboxylate (Compound 1-147)
Figure imgf000194_0001
SCHEME I
Method l-l Step i
Figure imgf000194_0002
[00336] Compound 1-147 was prepared using Method A, Steps 1-2 followed by Method I- E, Step 1 followed by Method I, Steps 1-2. METHOD I
Step 1 : 4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)benzoic acid
Figure imgf000195_0001
5-bromo-3-(6-methyl-1H-benzimidazol-2-yl)pyrazin-2-amine (1.855 g, 6.099 mmol) , 4- boronobenzoic acid (1.012 g, 6.099 mmol) and Na2CO3 (1.293 g, 12.20 mmol) suspended in MeCN (30 mL) / water (30 mL) . The mixture was degassed (5 x N 2 vacuum cycles) and Pd(PPh3)4 (704.8 mg, 0.6099 mmol) added. The mixture was degassed again and heated to 9O°C. No sign of product was observed therefore 25mL aliquots were heated in the microwave for lhour at 140°C which led to product formation. The mixture was allowed to cool and washed with DCM (x2). The aqueous layer was acidified to pH4 (IM HCl) and the resulting precipitate collected, washed with water and dried overnight under vacuum to give the product as a bright yellow solid, (1.30g, 62% Yield); MS (ES+) 346 Step 2 : tert-butyl 4-(4-(5-amino-6-(6-methyI-1H-benzo[d]imidazol-2-yl)pyrazin-2- yl)phenylcarbonyl)-l ,4-diazepane-l -carboxylate
Figure imgf000195_0002
[00337] To a solution of 4-[5-amino-6-(6-methyl-1H-benzimidazol-2-yl)pyrazin-2- yl]benzoic acid (108 mg, 0.3127 mmol) in DMSO (1 mL) were added tert-butyl 1 ,4- diazepane-l -carboxylate (187.9 mg, 0.9381 mmol), diethylcyanophosphonate (124.7 mg, 1 14.3 μL, 0.6879 mmol) and DIPEA (121.2 mg, 163.3 μL, 0.9381 mmol). The reaction mixture was heated at 80°C overnight, allowed to cool and. filtered and the resultant taken on to the next step without further purification (122mg, 75% Yield).
I H NMR (400.0 MHz, DMSO) d 1.43 (s, 9H), 1.59 (s, 1H), 1.79 (s, 1H), 2.47 (s, 3H), 3.39 -
3.73 (m, 8H), 5.80 (br s, 2H), 7.13 (m, 1H), 7.44 - 7.49 (m, 3H), 7.61 (d, 1H), 8.32 - 8.37 (m,
3H) and 8.85 (s, 1 H) ppm; MS (ES+) 528
[00338] Compounds 1-147 to 1-152 were all prepared using Method A, Steps 1-2 followed by Method 1-E, Step 1 followed by Method I, Steps 1-2.
Compound 148 (4-(5-amino-6-(6-methyl-l H-benzo[d]imidazol-2-yl)pyrazin-2-yl) phenyl)(4-(dimethylamino)piperidin- 1 -y l)methanone
I H NMR (400.0 MHz, DMSO) d 12.9 (2H, d), 9.78 (I H, s), 8.86 (I H, s), 8.37 (2H, d), 8.24
(I H, br s), 7.61 (I H, d), 7.54 (2H, d), 7.49 (I H, s), 7.13 (1H, d), 4.05-5.00 (4H, m), 3.79 (1H, m), 3.47 (I H, m), 3.14 (1H, m), 2.79 (3H, s), 2.77 (3H, s), 2.47 (3H, s), 2.02 (2H, m), 1.63
(2H, m) ppm; MS (ES+) 456
Compound 1-149 (4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-yl) phenyl)(piperazin-1-yl)methanone; MS (ES+) 414
Compound 1-150 (4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-yl) pheny l)(4-methy lpiperazin- 1 -y l)methanone
1H NMR (400.0 MHz, DMSO) d 12.96 (1H, br s), 10.16 (1H, s), 8.87 (1H, s), 8.40 (2H, d),
7.61-7.57 (3H, m), 7.49 (I H, s), 7.12 (I H, d), 5.2-3.81 (2H, m), 3.49-3.1 1 (6H, m), 2.85 (3H, s), 2.47 (3H, s) ppm; MS (ES+) 428
Compound 1-151 (4-(5-amino-6-(6-methyl-l H-benzo[d]imidazol-2-yl)pyrazin-2-yl) phenyl)(4-methyl-1,4-diazepan-1-yl)methanone
I H NMR (400.0 MHz, CD3OD) d 8.56 (1H, s), 8.22 (2H, d), 7.55-7.46 (3H, m), 7.39 (I H, s),
7.05 (I H, d), 3.81 -3.25 (1OH, m), 2.90 (3H, s), 2.20 (3H, s), 2.21-2.07 (2H, m) ppm; MS
(ES+) 442
[00339] Compound 1-152 4-(5-amino-6-(1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)-N-(2-
(pyrrolidin-l -yl)ethyl)benzamide; MS (ES+) 428 Example 10 : (4-(5-amino-6-(6-methyl-1H-benzofdlimidazol-2-yl)pyrazin-2-yl')phenyl') (1 ,4- diazepan-1-vQmethanone (Compound 1-153)
Figure imgf000197_0001
SCHEME I-J
Figure imgf000197_0002
METHOD I-J
Step 1: (4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)phenyl) (1,4- diazepan-1-yl)methanone
Figure imgf000197_0003
[00340] tert-butyl 4-[4-[5-amino-6-(6-methyl- 1 H-benzimidazol-2-yl)pyrazin-2- yl]benzoyl]-1,4-diazepane-1-carboxylate (117 mg, 0.2218 mmol) was dissolved in DCM (3 mL) and the mixture was cooled to 0°C. TFA (3 mL, 38.94 mmol) was added and the reaction mixture was allowed to warm to room temperature and stirred for a further 2 hours. Solvents were evaporated and the residue was dissolved in a mixture of MeCN and water (5mL/5mL) and submitted to Genevac evaporation to yield the product (1 19mg, 99% Yield). 1H NMR (400.0 MHz, CD3OD) d 2.18-2.04 (2H, m), 2.45 (3H, s), 3.B (3H, m), 3.44 (2H, m), 3.63 (2H, m), 3.82 (1H, m), 3.96 (2H, m), 7.15 (1H, d), 7.45 (1H, s), 7.55 (2H, d), 7.58 (1H, s), 8.59 (2H, d), 8.59(1 H, s) ppm; MS (ES+) 428
Example 1 1 : 3-amino-6-(4-(4-(dimethylamino)piperidine-1-carbonyl)pheny I)-N- phenylpyrazine-2-carboxamide (Compound 11-10)
Figure imgf000198_0001
SCHEME H-A
Figure imgf000199_0001
METHOD H-A: Step 1: Methyl 3-amino-6-bromopyrazine-2-carboxylate
Figure imgf000199_0002
[00341] A mixture of methyl 3-aminopyrazine-2-carboxylate (8.35 g, 54.53 mmol) and N- bromo-succinimide (9.705 g, 54.53 mmol) was stirred in MeCN (100 mL) at room temp overnight. The resultant precipitate was filtered, washed with MeCN and dried to give the desired product as a yellow solid (1 1.68 g, 92% Yield); 1H NMR (400.0 MHz, DMSO) 3.85 (s, 3H), 7.55 (br s, 2H) and 8.42 (s, 1H) ppm; MS (ES+) 233 Step 2: 3-amino-6-bromopyrazine-2-carboxylic acid
Figure imgf000199_0003
[00342] A mixture of methyl 3-amino-6-bromo-pyrazine-2-carboxylate (5.1 1 g, 22.02 mmol) and lithium hydroxide (2.637 g, 1 10.1 mmol) in MeOH (20 mL) and H2O (20 mL) was heated to 90 °C for 2 hours. The reaction mixture was allowed to cool and neutralised with HCl and the resultant precipitate collected by filtration. Taken on to the next step without further purification (4.8Og, 99% Yield).
Step 3 : S-amino-ό-bromo-N-phenylpyrazine-1-carboxamide
Figure imgf000200_0001
[00343] A mixture of S-amino-ό-bromo-pyrazine^-carboxylic acid (3.5 g, 16.05 mmol), l ,l'-carbonyldi imidazole (5.205 g, 32.10 mmol), DIPEA (2.282 g, 3.075 mL, 17.66 mmol) and DMAP (98.04 mg, 0.8025 mmol) were combined in DMSO (131 mL) and stirred for 30 min. Aniline (1.495 g, 1.463 mL, 16.05 mmol) was then added and the resulting solution stirred at RT for 18 hours. After this time water was added and the product collected by filtration to give a brown powder (3.5 g, 74% Yield).
1H NMR (400.0MHz, DMSO) d 7.04 (1H, m), 7.29 (2H, m), 7.72 (4H, m), 8.36 (1H, s),
10.22 (NH2) ppm; MS (ES+) 295.
Step 4 : 4-(5-amino-6-(phenylcarbamoyl)pyrazin-2-yl)benzoic acid
Figure imgf000200_0002
[00344] A mixture of 3-amino-6-bromo-N-phenyl-pyrazine-2-carboxamide (3.62 g, 12.35 mmol), 4-boronobenzoic acid (2.049 g, 12.35 mmol) and Na2CO3 (2.618 g, 24.70 mmol) was suspended in MeCN (60 mL) / water (60 mL) . The mixture was degassed (5 x N 2 vacuum cycles) and Pd(PPh^)4 (1.427 g, 1.235 mmol) added. The mixture was degassed again and heated to 9O°C. After 4 hours, the mixture was allowed to cool, concentrated to half its original volume and washed with DCM. The aqueous phase was acidified to pH4 (2M HCl) and the resulting precipitate collected, washed with water and dried overnight under vacuum to give the product as a bright yellow solid, (3.05g, 69% Yield). 1H NMR (400 MHz, DMSO) d 7.17 (I H, t), 7.41 (2H, t), 7.83 (4H, d), 8.03 (2H, d), 8.37 (2H, d), 9.01 (I H, s), 10.45 (I H, s), 13.03 (1H, brs) ppm; MS (ES+) 335
Step 5 : 3-amino-6-(4-(4-(dimethyIamino)piperidine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide
Figure imgf000201_0001
[00345] N,N-dimethylpiperidin-4-amine (57.54mg, 0.449 mmol) was weighed into a greenhouse tube and treated with a solution of 4-(5-amino-6-(phenylcarbamoyl)pyrazin-2- yl)benzoic acid (50mg, 0.150 mmol), CDI (48.5 lmg, 0.299 mmol) and DMAP (1.82mg, 0.015 mmol) in DMSO (ImL of a stock solution). DIPEA (78.2uL, 0.449 mmol) was then added and the mixture stirred at 38°C for 6 hours. The reaction mixture was filtered and the resultant residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, lOuM, IOOA column, gradient 10% - 95%B (solvent A: 0.05% TFA in water, solvent B: CH3CN) over 16 minutes at 25mL/min]. The fractions were freeze-dried to give the title compound as a solid (54.65, 80% Yield). (ES+) 445
[00346] The following compounds were all prepared using the above sequence: Compound H-I: 6-(4-(1,4-diazepane-1-carbonyl)phenyl)-3-amino-N-phenylpyrazine-2- carboxamide I H NMR (400.0 MHz, DMSO) d 1.44 - 1.47 (m, 1H), 1.53 - 1.58 (m, I H), 2.57 - 2.61 (m, I H), 2.62 - 2.69 (m, 2H), 2.74 - 2.80 (m, I H), 3.15 - 3.20 (m, 2H), 3.40 - 3.46 (m, 2H), 6.91 - 6.96 (m, I H), 7.15 - 7.19 (m, 2H), 7.23 - 7.28 (m, 2H), 7.51 (br s, 2H), 7.58 - 7.60 (m, 2H), 8.05 - 8.08 (m, 2H), 8.74 (s, I H) and 10.20 (s, 1H) ppm; (ES+) 417 Compound II-2: 3-amino-N-phenyl-6-(4-(2-(pyrrolidin-1-yl)ethylcarbamoyl)phenyl) pyrazine-2-carboxamide; 1H NMR (400 MHz, DMSO) d 1.80 (4H, vbrs), 3.51 (2H, brs),
7.18 (I H, t), 7.41 (2H, t), 7.81-7.85 (4H, m), 7.95 (2H, d), 8.35 (2H, d), 8.65 (I H, brs), 9.02
(I H, s), 10.44 (1 H, s) ppm; (ES+) 431
Compound II-3: 3-amino-N-phenyl-6-(4-(2-(piperidin-1-yl)ethylcarbamoyl) phenyl) pyrazine-2-carboxamide 1H
NMR (400 MHz, DMSO) d 1.30-2.40 (2H, m), 1.46-1.53 (4H, m), 2.33 (4H, m), 2.45 (2H, t),
3.37-3.44 (2H, m), 7.16 (I H, t), 7.41 (2H, t), 7.79 (2H, brs), 7.81 (2H, d), 7.95 (2H. d), 8.34
(2H, d), 8.48 (I H, t), 9.00 (I H, s), 10.45 (1H, s) ppm; (ES+) 445
Compound II-4: 3-amino-N-phenyl-6-(4-(2-(pyrrolidin-1-ylmethyl)pyrrolidine-1- carbonyl)phenyl)pyrazine-2-carboxamide (ES+) 471
Compound II-5: 3-amino-6-(4-((2-(dimethylamino)ethyl)(methyl)carbamoyl) phenyl)-N- phenylpyrazine-2-carboxamide; (ES+) 419
Compound II-6: 3-amino-N-phenyl-6-(4-(4-(pyrro!idin-1-yl)piperidine-1- carbonyl)phenyl)pyrazine-2-carboxamide (ES+) 471
Compound II-7: 3-amino-6-(4-(4-methylpiperazine-l -carbonyl)phenyl)-N-phenylpyrazine-
2-carboxamide; (ES+) 417
Compound II-8: 3-amino-N-phenyl-6-(4-(piperazine-1-carbonyl)phenyl)pyrazine-2- carboxamide; (ES+) 403
Compound II-9: 3-amino-6-(4-(3-(dimethylamino)pyrrolidine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide; (ES+) 431
Compound 11-11: 3-amino-6-(4-(4-(2-cyanoethyl)piperazine-1-carbonyl)phenyl)-N- phenylpyrazine-2-carboxamide ; (ES+) 456
Example 12: (4-(5-amino-6-(6-methyl-1H-benzord]imidazol-2-v0pyrazin-2-yl')phenyl') (1,4- diazepan-1-yl)methanone (Compound III-l *)
Figure imgf000202_0001
SCHEME IH-A
Step 4
Figure imgf000203_0001
METHOD III-A
Step 1 : Methyl 3-amino-6-bromopyrazine-2-carboxylate
Figure imgf000203_0002
[00347] A mixture of methyl 3-aminopyrazine-2-carboxylate (8.35 g, 54.53 mmol) and N- bromo-succinimide (9.705 g, 54.53 mmol) was stirred in MeCN (100 mL) at room temp overnight. The resultant precipitate was filtered, washed with MeCN and dried to give the desired product as a yellow solid (1 1.68 g, 92% Yield)
1H NMR (400.0 MHz, DMSO) 3.85 (s, 3H), 7.55 (br s, 2H) and 8.42 (s, 1H) ppm; MS (ES+)
233 Step 2 : 3-amino-6-bromopyrazine-2-carboxylic acid
Figure imgf000204_0001
[00348] A mixture of methyl 3-amino-6-bromo-pyrazine-2-carboxylate (5.11 g, 22.02 mmol) and lithium hydroxide (2.637 g, 1 10.1 mmol) in MeOH (20 mL) and H2O (20 mL) was heated to 90 °C for 2 hours. The reaction mixture was allowed to cool and neutralised with HCl and the resultant precipitate collected by filtration. Taken on to the next step without further purification (4.8Og, 99% Yield).
Step 3 : 5-bromo-3-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-amine
Figure imgf000204_0002
[00349] A mixture of 3-amino-6-bromo-pyrazine-2-carboxylic acid (5.52g, 25.32 mmol), 4-methylbenzene-1,2-diamine,(3.09g, 25.32 mmol), diethoxyphosphorylformonitrile (4.54, 27.85 mmol) and triethylamine (7.06 mL, 50.64 mmol) in DME (30 mL) was heated in the microwave at 170°C for 60 minutes. The mixture was. diluted with Ethyl acetate, washed with water followed by aqueous NaHCCh then brine. After drying over MgSO4, the mixture was decolourised with charcoal and filtered through silica gel. After concentration, the mixture was filtered to give gold coloured crystals (4.005g, 52% Yield). MS (ES+) 305
Step 4 : 4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)benzoic acid
Figure imgf000205_0001
[00350] 5-bromo-3-(6-methyl-l H-benzimidazol-2-yl)pyrazin-2-amine (1.855 g, 6.099 mmol) , 4-boronobenzoic acid (1.012 g, 6.099 mmol) and Na2CO3 (1.293 g, 12.20 mmol) suspended in MeCN (30 mL) / water (30 mL) . The mixture was degassed (5 x N 2 vacuum cycles) and Pd(PPh3)4 (704.8 mg, 0.6099 mmol) added. The mixture was degassed again and heated to 9O°C. No sign of product was observed therefore 25mL aliquots were heated in the microwave for lhour at 140°C which led to product formation. The mixture was allowed to cool and washed with DCM (x2). The aqueous layer was acidified to pH4 (IM HCl) and the resulting precipitate collected, washed with water and dried overnight under vacuum to give the product as a bright yellow solid, (1.30g, 62% Yield). MS (ES+) 346
Step 5 : tert-butyl 4-(4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2- yl)phenylcarbonyl)-1,4-diazepane-1-carboxylate
Figure imgf000205_0002
[00351] To a solution of 4-[5-amino-6-(6-methyl-1H-benzimidazol-2-yl)pyrazin-2- yl]benzoic acid (108 mg, 0.3127 mmol) in DMSO (1 mL) were added tert-butyl 1,4- diazepane-1-carboxylate (187.9 mg, 0.9381 mmol), diethylcyanophosphonate (124.7 mg, 1 14.3 μL, 0.6879 mmol) and DIPEA (121.2 mg, 163.3 μL, 0.9381 mmol). The reaction mixture was heated at 80°C overnight, allowed to cool and filtered and the resultant taken on to the next step without further purification (122mg, 75% Yield).
1H NMR (400.0 MHz, DMSO) d 1.43 (s, 9H), 1.59 (s, 1H), 1.79 (s, 1H), 2.47 (s, 3H), 3.39 - 3.73 (m, 8H), 5.80 (br s, 2H), 7.13 (m, 1H), 7.44 - 7.49 (m, 3H), 7.61 (d, 1H), 8.32 - 8.37 (m, 3H) and 8.85 (s, I H) ppm; MS (ES+) 528
Step 6 : (4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yI)pyrazin-2-yl)phenyl) (1,4- diazepan-1-yl)methanone (Compound III-l)
Figure imgf000206_0001
[00352] tert-butyl 4-[4-[5-amino-6-(6-methyl-l H-benzimidazol-2-yl)pyrazin-2- yl]benzoyl]-l ,4-diazepane-1-carboxylate (1 17 mg, 0.2218 mmol) was dissolved in DCM (3 mL) and the mixture was cooled to O°C. TFA (3 mL, 38.94 mmol) was added and the reaction mixture was allowed to warm to room temperature and stirred for a further 2 hours. Solvents were evaporated and the residue was dissolved in a mixture of MeCN and water (5mL/5mL) and submitted to Genevac evaporation to yield the product (1 19mg, 99% Yield). 1H NMR (400.0 MHz, CD3OD) d 2.18-2.04 (2H, m), 2.45 (3H, s), 3.33 (3H, m), 3.44 (2H, m), 3.63 (2H, m), 3.82 (I H, m), 3.96 (2H, m), 7.15 (1H, d), 7.45 (1H, s), 7.55 (2H, d), 7.58 (I H, s), 8.59 (2H, d), 8.59(1 H, s) ppm; MS (ES+) 428
The following compounds were all prepared using the above sequence Step 1 -5:
Compound III-2 (4-(5-amino-6-(6-methyl-l H-benzo[d]imidazol-2-yl)pyrazin-2-yl) phenyl)(4-(dimethylamino)piperidin- 1 -yl)methanone
I H NMR (400.0 MHz, DMSO) d 12.9 (2H, d), 9.78 (I H, s), 8.86 (I H, s), 8.37 (2H, d), 8.24 (I H, br s), 7.61 (I H, d), 7.54 (2H, d), 7.49 (I H, s), 7.13 (I H, d), 4.05-5.00 (4H, m), 3.79 (I H, m), 3.47 (1H, m), 3.14 (1H, m), 2.79 (3H, s), 2.77 (3H, s), 2.47 (3H, s), 2.02 (2H, m), 1.63
(2H, m) ppm; MS (ES+) 456
Compound III-3: (4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-yl) phenyl)(piperazin-1-yl)methanone; MS (ES+) 414
Compound III-4: (4-(5-amino-6-(6-methyl-l H-benzo[d]imidazol-2-yl)pyrazin-2-yl) phenyl)(4-methylpiperazin-l -yl)methanone
I H NMR (400.0 MHz, DMSO) d 12.96 (I H, br s), 10.16 (I H, s), 8.87 (I H, s), 8.40 (2H, d),
7.61-7.57 (3H, m), 7.49 (I H, s), 7.12 (I H, d), 5.2-3.81 (2H, m), 3.49-3.1 1 (6H, m), 2.85 (3H, s), 2.47 (3H, s) ppm; MS (ES+) 428
Compound III-5: (4-(5-amino-6-(6-methyl-1H-benzo[d]imidazol-2-yl)pyrazin-2-yl) phenyl)(4-methy 1- 1 ,4-diazepan- 1 -y l)methanone
1H NMR (400.0 MHz, CD3OD) d 8.56 (1H, s), 8.22 (2H, d), 7.55-7.46 (3H, m), 7.39 (1H, s),
7.05 (1H, d), 3.81-3.25 (1OH, m), 2.90 (3H, s), 2.20 (3H, s), 2.21-2.07 (2H, m) ppm; MS
(ES+) 442
Compound III-6: 4-(5-amino-6-(1H-benzo[d]imidazol-2-yl)pyrazin-2-yl)-N-(2-(pyrrolidin- l-yl)ethyl)benzamide: MS (ES+) 428
Example IA : 4-(5-amino-6-(5-phenvI-1,3,4-oxadiazol-2-y0pyrazin-2-vlWVJV- dimethylbenzamide (Compound IA-23)
SCHEME
Figure imgf000207_0001
Compound IA-23 was prepared using Method IV-A, Steps 1-3
METHOD IV-A :
Step 1 : 3-amino-6-bromo-.N-(phenylcarbonyl)pyrazine-2-carbohydrazide [00353] TBTU (22.09 g, 68.80 mmol) and triethylamine (4.642 g, 6.394 mL, 45.87 mmol) were added to a suspension of 3-amino-6-bromo-pyrazine-2-carboxylic acid (10 g, 45.87 mmol) and benzohydrazide (7.494 g, 55.04 mmol) in DMF (100.0 mL) and the resulting solution stirred at ambient temperature for 48 hours and then poured into water (40OmL) with vigorous stirring. This was allowed to stir for 30 minutes, filtered and washed with water. The moist solid was dissolved in hot EtOAc, dried (MgSO-i), filtered and concentrated in vacuo and the resultant solid dried under vacuum to give the desired product (11.34g, 73% Yield). 1H NMR (400.0 MHz, DMSO) d 7.51 (2H, m), 7.61 (1H, m), 7.69 (2H, br s), 7.92 (2H, m), 8.44 (1H, s), 10.48 (1H, br s), 10.54 (1H, br s) ppm; MS (ES+) 338.01
Step 2 : 5-bromo-3-(5-phenyl-1,3,4-oxadiazol-2-yI)pyrazin-2-amine
[00354] Polyphosphoric acid (314 g) was heated to 100°C and treated portionwise with 3- amino-N'-benzoyl-6-bromopyrazine-2-carbohydrazide (22.5 g, 66.94 mmol) over a period of 20 minutes. The reaction was allowed to stir at 110-120°C for 6 hours and then allowed to cool and treated with ice/water and stirred. The resultant solid was filtered and washed with water. It was taken into EtOAc, washed with water and adjusted to pH 1 1 (NaOH solution) and then washed with brine, dried (MgSOO and concentrated in vacuo to give the desired product (13.25g, 62% Yield). 1H NMR (400.0 MHz, DMSO) d 7.69 (3H, m), 7.86 (2H, br s), 8.16 (2H, m), 8.50 (1H, s) ppm; MS (ES+) 319.89
Step 3 : 4-(5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl)-ΛyV- dimethylbenzamide
[00355] 5-bromo-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-amine (150 mg, 0.4715 mmol), [4-(dimethylcarbamoyl)phenyl]boronic acid (91.00 mg, 0.4715 mmol), sodium carbonate (99.95 mg, 0.9430 mmol) and palladium; triphenylphosphane (54.48 mg, 0.04715 mmol) in a mixture of acetonitrile (1.5 mL) and water (1.5 mL) was heated at 1 10°C in the microwave for 30 minutes. The reaction was diluted with water and EtOAc and the layers separated. The combined organics were dried (MgSO,j), filtered and concentrated in vacuo. The residue was purified by column chromatography (ISCO Companion™, 12g column, 0- 100% EtO Ac/Petroleum ether) to give the desired product (102.8mg, 56% Yield). 1H NMR (400.0 MHz, DMSO) d 2.98 (6H, m), 7.55 (2H, m), 7.69-7.71 (3H, m), 7.83 (2H, br s), 8.17- 8.20 (4H, m), 9.00 (1H, s) ppm; MS (ES+) 387.13
[00356] The following compounds were all prepared using a method similar to the one described for Compound IA-23 above.
Compound IA-90 5-(4-isopropylsulfinylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin- 2-amine I H NMR (400.0 MHz, DMSO) d 0.95 (d, 3H), 1.25 (d, 3H), 2.98-3.02 (m, 1H), 7.6- 8.0 (m, 6H), 8.25 (d, 2H), 8.35 (d, 2H) and 9.05 (s, 1H) ppm; MS (ES+) 406.2
Compound IA-112 5-[4-(azetidin-1-ylsulfonyl)phenyl]-3-(5-pheny 1-1 ,3, 4-oxadiazol-2- yl)pyrazin-2-amine I H NMR (400.0 MHz, CDCl3) d 2.0-2.2 (m, 2H), 3.0-3.2 (m, 2H), 3.83- 3.9 (m, 4H), 7.6-7.7 (m, 3H), 8.05 (d, 2H), 8.25-8.3 (m, 4H) and 8.85 (s, I H) ppm; MS (ES+) 435.2
Compound IA-134 3-(5-phenyl-l ,3',4-oxadiazol-2-yl)-5-(2-phenylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 7.2-7.28 (2H,m), 7.3-7.35 (1H,m), 7.45-7.5 (1H,m), 7.55-7.6 (3H,m), 7.65-7.7 (3H,m), 7.75-7.8 (1H,m), 7.72 (l H,s) and 8.1-8.15 (2H,m) ppm; MS (ES+) 392.3
Compound IA-184 5-(2-ethylsulfanylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- amine 1 H NMR (400.0 MHz, CDCl3) d 1.25 (t, 3H), 3.95 (q, 2H), 7.4-7.5 (m, 2H), 7.5-7.65 (m, 5H), 8.25 (d, 2H) and 8.6 (s, I H) ppm; MS (ES+) 376.2
Compound IA-207 5-(2-oxazol-5-ylphenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- amine 1 H NMR (400.0 MHz, DMSO) d 7.6-7.8 (m, 9H), 8.1-8.13 (m, 2H), 8.15 (s, 1 H) and 8.18 (s, I H) ppm; MS (ES+) 383.1
Compound IA-229 5-(2-isopropylsulfanylphenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin- 2-amine 1H NMR (400.0 MHz, CDCl3) d 1.35 (d, 6H), 3.4-3.5 (m, 1H), 7.0 (br s, 2H), 7.4- 7.45 (m, 2H), 7.5-7.65 (m, 5H), 8.2-8.25 (m, 2H) and 8.55 (s, 1H) ppm; MS (ES+) 390.2
Example 2A : 4-(5-amino-6-(5-(3-fluorophenvD-1.3.4-oxadiazol-2-vl')Dvrazin-2-vO-N.N- dimethylbenzamide (Compound IA-40) SCHEME
Figure imgf000210_0001
Compound IA-40 was prepared using Method IV-B, Steps 1-4
METHOD IV-B :
Step 1 : Methyl 3-amino-6-(4-(dimethylcarbamoyl)phenyl)pyrazine-2-carboxylate
[00357] Methyl 3-amino-6-bromo-pyrazine-2-carboxylate (625.1 mg, 2.694 mmol), [4- (dimethylcarbamoyl)phenyl]boronic acid (520 mg, 2.694 mmol), sodium carbonate (571.1 mg, 5.388 mmol) and palladium; triphenylphosphane (311.3 mg, 0.2694 mmol) in a mixture of acetonitrile (3 mL) and water (3 mL) was heated at 110°C in the microwave for 30 minutes. The reaction was diluted with EtOAc and water and the layers separated. The aqueous later was extracted further with EtOAc (2x) and the combined organics dried (MgSO^, filtered and concentrated in vacuo. The residue was purified by column chromatography (ISCO Companion™, 4Og column, 0-100% EtO Ac/Petroleum ether) to give the desired product as a yellow solid (375mg, 46% Yield). 1H NMR (400.0 MHz, DMSO) d 3.02 (3H, s), 3.15 (3H, s), 4.04 (3H, s), 7.54 (2H, m), 7.97 (2H, m), 8.71 (1H, s) ppm; MS (ES+) 301.13
Step 2 : 3-amino-6-(4-(dimethylcarbamoyl)phenyl)pyrazine-2-carboxylic acid
[00358] To a solution of methyl 3-amino-6-[4-(dimethylcarbamoyl)phenyl]pyrazine-2- carboxylate (390 mg, 1.299 mmol) in MeOH (2.127 mL) was added a solution of NaOH (649.5 μL of 2 M, 1.299 mmol) in H2O (2.127 mL). The resulting solution was heated to 60°C for 2 hours and then allowed to cool and neutralised with HCl. The resultant precipitate was collected and washed with ether and dried (340mg, 91% Yield). MS (ES+) 287.08 Step 3 : 4-(5-amino-6-(2-(3-fluorophenylcarbonyl)hydrazinecarbonyl)pyrazin-2-yl)-N,N- dimethylbenzamide
[00359] 3-fluorobenzohydrazide (80.77 mg, 0.5240 mmol) was added to a solution of 3- amino-6-[4-(dimethylcarbamoyl)phenyl]pyrazine-2-carboxylic acid (150 mg, 0.5240 mmol), triethylamine (53.02 mg, 73.03 μL, 0.5240 mmol) and TBTU (252.4 mg, 0.7860 mmol) in DMF (3.000 mL) and the resulting solution stirred at RT for 2 hours. The reaction was diluted with EtOAc and water and the layers separated. The aqueous layer was extracted further with EtOAc (2 x) and the combined organics washed with water (3 x), dried (MgSO4), filtered and concentrated to give the desired product as a yellow solid (172mg, 78% Yield). MS (ES+) 423.13
Step 4 : 4-(5-amino-6-(5-(3-fluorophenyl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl)-N,N- dimethylbenzamide
[00360] A suspension of 4-[5-amino-6-[[(3-fluorobenzoyl)amino]carbamoyl]pyrazin-2- yl]-Ν,Ν-dimethyl-benzamide (127 mg, 0.3007 mmol) in anhydrous acetonitrile (2.540 mL) cooled in an ice bath, was treated with DIPEA (1 16.6 mg, 157.1 μL, 0.9021 mmol) followed by dibromo(triphenyl)phosphorane (165.0 mg, 0.3909 mmol) portionwise. The reaction mixture was then placed under nitrogen and allowed to stir for 10 minutes. The resultant precipitate was isolated by filtration, washed with ether and dried to give the impure desired product. The material was purified further by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. The fractions were collected, passed through a sodium bicarbonate cartridge and freeze-dried to give the title compound as a yellow solid (58.4mg, 48% Yield). 1H NMR (400.0 MHz, DMSO) d 2.98 (6H, m), 7.55-7.61 (3H, m), 7.73-7.85 (3H, m), 7.96 (1H, m), 8.02 (1H, m), 8.19 (2H, m), 9.01 (1H, s) ppm; MS (ES+) 405.16
[00361] The following compounds were all prepared using a method similar to the one described for Compound IA-40 above.
Compound IA-195 4-[5-amino-6-[5-(3-methoxyphenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.98 (m, 6H), 3.90 (s, 3H), 7.28 (m, I H), 7.55-7.57 (m, 2H), 7.60-7.65 (m, 2H), 7.74 (m, 1H), 8.17 (m, 2H) and 9.00 (I H, s) ppm; MS (ES+) 417.17
Compound IA-233 4-[5-amino-6-[5-[2-(trifluoromethyl)phenyl]-1,3,4-oxadiazol-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.98 (m, 6H), 7.51 (m, 2H), 7.80 (br s, 1H), 7.93-8.01 (m, 2H), 8.09-8.14 (m, 3H), 8.19 (m, 1H) and 9.03 (s, 1H) ppm; MS (ES+) 455.12
Example 3A : 4-(5-amino-6-(5-(benzvlaminoM.3.4-oxadiazol-2-v0pvrazin-2-vlWVJV- dimethylbenzamide (Compound IA-55)
Figure imgf000212_0001
Compound IA-55 was prepared using Method IV-C, Steps 1-3
METHOD IV-C :
Step 1: Methyl 3-amino-6-(4-(dimethylcarbamoyl)phenyl)pyrazine-2-carboxylate
[00362] Methyl 3-amino-6-bromo-pyrazine-2-carboxylate (6.012 g, 25.91 mmol), [4- (dimethylcarbamoyl)phenyl]boronic acid (5 g, 25.91 mmol), sodium carbonate (5.492 g, 51.82 mmol) and Pd(PPh3)4 (2.994 g, 2.591 mmol) in a mixture of acetonitrile (28.85 mL) and water (28.85 mL) was heated at HO°C. The reaction mixture was allowed to cool and the residual solid filtered off. The filtrate was diluted with EtOAc and water and the layers separated. The aqueous layer was acidified to pH4 (by addition of IM HCl) and then extracted with dichloromethane (3 x), dried (MgSO4), filtered and concentrated in vacuo to leave the product as a yellow solid. The ethyl acetate extracts were concentrated in vacuo and combined with the filtered solid. Preabsorbed onto silica and purified by column chromatography on silica using the companion eluting with ethyl acetate / petroleum ether (0-100% EtOAc). Product eluted with 100% EtOAc. Product fractions combined and cone in vacuo to leave a yellow solid (1.95g, 50% Yield). 1H NMR (400.0 MHz, DMSO) d 3.02 (3H, s), 3.15 (3H, s), 4.04 (3H, s), 7.54 (2H, m), 7.97 (2H, m), 8.71 (1H, s) ppm; MS (ES+) 301.13
Step 2 : 4-(5-amino-6-(hydrazinecarbonyl)pyrazin-2-yl)-N,N-dimethylbenzamide
[00363] To a stirred solution of methyl 3-amino-6-[4-(dimethylcarbamoyl) phenyl]pyrazine-2-carboxylate (1.701 1 g, 5.664 mmol) in EtOH (10.21 mL) was added hydrazine (726.1 mg, 71 1.2 μL, 22.66 mmol). The resultant solution was heated to reflux for 30 minutes and then allowed to cool to RT. The precipitate was filtered off and dried (1.47g, 87% Yield). I H ΝMR (400.0 MHz, DMSO) d 2.96 (s, 3H), 3.00 (s, 3H), 4.58 (d, J = 4.4 Hz, 2H), 7.46 (d, J = 8.4 Hz, 2H), 8.27 - 8.29 (m, 2H), 8.88 (s, 1H) and 10.09 (s, 1H) ppm; MS (ES+) 301.13
Step 3 : 4-(5-amino-6-(5-(benzylamino)-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl)-N,N- dimethylbenzamide
[00364] A mixture of 4-(5-amino-6-(hydrazinecarbonyl)pyrazin-2-yl)-Ν,Ν- dimethylbenzamide (75 mg, 0.2497 mmol), isothiocyanatomethylbenzene (37.26 mg, 33.12 μL, 0.2497 mmol) and dry THF (1.500 mL) was stirred at RT for 4 hours. The reaction mixture was evaporated to dryness and treated with DCM followed by EDC (71.81 mg, 0.3746 mmol) and the resultant mixture allowed to stir at RT overnight. The reaction mixture was filtered and the resultant green precipitate dried under vacuum (78 mg, 73% Yield). I H NMR (400.0 MHz, DMSO) d 2.96 (s, 3H), 3.00 (s, 3H), 4.50 (d, J = 6.1 Hz, 2H), 7.29 (d, J = 7.2 Hz, I H), 7.35 - 7.42 (m, 4H), 7.51 - 7.53 (m, 2H), 7.65 (br s, 2H), 8.06 (dd, J = 1.5, 6.9 Hz, 2H) and 8.81 (d, J = 12.4 Hz, 2H) ppm; MS (ES+) 416.2
[00365] The following compounds were all prepared using a method similar to the one described for Compound IA-55 above.
Compound IA-103 4-[5-amino-6-[5-(2-methoxyanilino)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 3.03 (s, 3H), 3.07 (s, 3H), 3.94 (s, 3H), 7.07 - 7.10 (m, 1H), 7.15-7.17 (m, 2H), 7.59 (d, 2H), 7.75 (br s, 2H), 8.12-8.19 (m, 3H), 8.94 (s, 1H) and 10.17 (s, 1H) ppm; MS (ES+) 432.16 Compound IA-129 4-[5-amino-6-[5-[[(l S)-1-(4-chlorophenyl)ethyl]amino]-1,3,4-oxadiazol- 2-yl]pyrazin-2-yl]-N,N-dimethyl-benzamide 1 H NMR (400.0 MHz, DMSO) d 1.50 (d, 3H), 2.96 (s, 3H), 3.01 (s, 3H), 4.83 (d, I H), 7.40-7.47 (m, 4H), 7.51-7.54 (m, 4H), 8.06 (d, 2H), 8.81 (s, 1 H) and 8.90 (br s, I H) ppm; MS (ES+) 464.16
Compound IA-156 4-[5-amino-6-[5-(phenethylamino)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.77 (s, 1H), 2.94 (t, 4H), 3.00 (s, 3H), 3.04 (s, 1H), 3.51-3.56 (m, 2H), 7.22-7.24 (m, 1H), 7.28 - 7.34 (m, 4H), 7.52 (d, 2H), 7.61 (s, 1H), 8.05-8.07 (m, 2H), 8.32 (t, I H) and 8.81 (s, 1H) ppm; MS (ES+) 430.2
Compound IA-163 4-[5-amino-6-[5-(cyclohexylamino)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide 1 H NMR (400.0 MHz, DMSO) d 1.17 - 1.19 (m, 1H), 1.30-1.35 (m, 4H), 1.57-1.60 (m, I H), 1.74-1.76 (m, 2H), 1.99 (s, 2H), 2.96 (s, 3H), 3.00 (s, 3H), 3.48 (br s, I H), 7.52 (d, 2H), 7.62 (br s, 2H), 8.06 (d, 2H), 8.20 (d, 1H) and 8.81 (s, I H) ppm; MS (ES+) 408.22
Compound IA-254 4-[5-amino-6-[5-(3-cyanoanilino)-l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.97 (s, 3H), 3.01 (s, 3H), 7.54 (t, 3H), 7.63 (t, 1H), 7.75 (br s, I H), 7.91 (dd, 2H), 8.09-8.13 (m, 3H), 8.91 (s, 1H) and 1 1.51 (s, I H) ppm; MS (ES+) 427.15
Compound IA-278 4-[6-(5-acetamido-l ,3,4-oxadiazol-2-yl)-5-amino-pyrazin-2-yl]-N,N- dimethyl-benzamide I H NMR (400.0 MHz, DMSO) d 2.20 (s, 3H), 2.96 (s, 3H), 3.01 (s, 3H), 7.54 (d, 2H), 7.66 (br s, 2H), 8.08 (d, 2H), 8.92 (s, 1H) and 1 1.92 (s, 1H) ppm; MS (ES+) 368.13
Compound IA-287 4-[5-amino-6-(5-benzamido-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N,N- dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.96 (s, 3H), 3.00 (s, 3H), 3.31 (s, 1H), 7.52-7.61 (m, 4H), 7.69 (t, 2H), 8.06-8.12 (m, 4H), 8.95 (s, 1H) and 12.35 (br s, 1H) ppm; MS (ES+) 430.14
Example 4A : (4-(5-amino-6-(5-phenvI-1.3.4-thiadiazol-2-vDpvrazin-2-vπphenvn(1.4- diazepan-1-yl)methanone (Compound IA-68) SCHEME
Figure imgf000215_0001
Compound IA-68 was prepared using Method IV-D, Steps 1-2.
METHOD IV-D :
Step 1 : 5-bromo-3-(5-phenyl-1,3,4-thiadiazol-2-yl)pyrazin-2-amine
[00366] S-amino-ό-bromo-pyrazine^-carboxylic acid (1.000 g, 4.588 mmol) and benzenecarbothiohydrazide (759.1 mg, 4.588 mmol) were suspended in acetonitrile (25.00 mL), cooled in an ice bath and then treated with dibromo-triphenyl-phosphorane (4.453 g, 10.55 mmol) The reaction mixture was allowed to stir in an ice bath for 2 hours and then DIPEA (1.778 g, 2.396 mL, 13.76 mmol) was added slowly at 10°C. The reaction was left to stir at 0-10°C for a further hour and the resultant precipitate was isolated by filtration, washed with a small amount of acetonitrile and dried (659mg, 43% Yield). MS (ES+) 335.93
Step 2 : (4-(5-amino-6-(5-phenyl-1,3,4-thiadiazol-2-yl)pyrazin-2-yl)phenyl)(1,4-diazepan-l - yl)methanone
[00367] 5-bromo-3-(5-phenyl-l ,3,4-thiadiazol-2-yl)pyrazin-2-amine (70 mg, 0.1257 mmol) and [4-(4-tert-butoxycarbonyl-l ,4-diazepane-1-carbonyl)phenyl]boronic acid (43.77 mg, 0.1257 mmol) (60% pure) were taken into dioxane (700.1 μL), treated with Na2CO3 (125.7 μL of 2 M, 0.2514 mmol) and degassed/nitrogen flushed (5x). The reaction was then treated with palladium; triphenylphosphane (14.53 mg, 0.01257 mmol), degassed again and heated in the microwave at 140°C for 30 minutes. The reaction was treated with EtOAc and brine, the organics separated, dried over MgSO4, filtered and concentrated under vacuum. The product was purified by column chromatography eluting 50% EtOAc/Petroleum ether followed by 10% MeOH/DCM to give the desired product which was dissolved in DCM (2.000 mL) and treating with TFA (2.960 g, 2.000 mL, 25.96 mmol). After stirring at RT for 30 minutes and concentration, the residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. The fractions were collected, passed through a sodium bicarbonate cartridge and freeze-dried to give the title compound (42mg, 74% Yield). 1H NMR (400.0 MHz, DMSO) d 1.60 (1H, m), 1.77 (1H, m), 2.72-2.39 (4H, m_, 3.40 (2H, m), 3.60-3.67 (2H, m), 7.52 (2H, d), 7.58-7.65 (3H, m), 7.99 (1H, m), 8.00 (2H, br s), 8.10-8.14 (3H, m), 8.95 (I H, s); MS (ES+) 458.07
Example 5 : 4-(5-amino-6-(5-phenvl-1.2.4-oxadiazoI-3-yl)pyrazin-2-vl)phenyl)(1,4- diazepan-1-yl)methanone (Compound IA-2)
SCHEME
Step 4
Figure imgf000216_0001
Compound IA-2 was prepared using Method IV-E, Steps 1-5.
METHOD IV-E
Step 1 : 3-amino-6-bromo-N-hydroxypyrazine-2-carboxirnidamide [00368] A mixture of 3-amino-6-bromo-pyrazine-2-carbonitrile (1 g, 5.025 mmol) was dissolved in MeOH (20.00 mL) and cooled to O°C. Hydroxylamine hydrochloride (349.2 mg, 5.025 mmol) and triethylamine (508.5 mg, 700.4 μL, 5.025 mmol) were added and the reaction allowed to warm to ambient temperature. After 2 hours the resultant precipitate was filtered off and dried (898mg, 77% Yield). MS (ES+) 234.89
Step 2 : 3-amino-6-bromo-N-(phenylcarbonyloxy)pyrazine-2-carboximidamide
[00369] 3-amino-6-bromo-Ν'-hydroxypyrazine-2-carboximidarnide (890 mg, 3.836 mmol) was suspended in dichloromethane (1 1.56 mL) and treated with triethylamine (427.0 mg, 588.2 μL, 4.220 mmol) followed by benzoyl chloride (593.2 mg, 489.8 μL, 4.220 mmol) The reaction mixture was allowed to stir for 1 hour and concentrated in vacuo. The resultant residue was triturated with methanol to give the desired product as a pale beige solid (891 mg, 69% Yield). I H NMR (400.0 MHz, DMSO) d 7.55 (2H, m), 7.65 (1H, m), 7.90 (2H, br s), 8.28 (2H, m), 8.33 (1H, s); MS (ES+) 337.87
Step 3 : 5-bromo-3-(5-phenyl-1,2,4-oxadiazol-3-yl)pyrazin-2-amine
[00370] 3-amino-N'-(benzoyloxy)-6-bromopyrazine-2-carboximidamide (890 mg, 2.648 mmol) and polyphosphonic acid (3.560 mL) were mixed and the reaction heated to 7O°C. Further polyphosphonic acid (8.900 mL) was added and the reaction allowed to stir for a further 3 hours at 70°C. The mixture was then allowed to cool to RT, diluted with water and neutralised by the portionwise addition of solid NaHCθ3. The resulting precipitate was isolated by filtration and dried (643mg, 76% Yield). I H NMR (400.0 MHz, DMSO) d 7.49 (2H, br s), 7.69 (2H, m), 7.77 (I H, m), 8.28 (2H, m), 8.43 (1H, s); MS (ES+) 319.89
Step 4: 4-(5-amino-6-(5-phenyl-l ,2,4-oxadiazol-3-yl)pyrazin-2-yl)benzoic acid
[00371] 5-bromo-3-(5-pheny 1- 1 ,2,4-oxadiazol-3-y l)pyrazin-2-amine (200 mg, 0.6287 mmol) 4-carboxyphenylboronic acid (104.3 mg, 0.6287 mmol) and Na2CO3 (133.2 mg, 1.257 mmol) were suspended in MeCN (3.314 mL) / water (3.314 mL) and the mixture de-gassed (x5) and treated with Pd(PPh3)4 (72.65 mg, 0.06287 mmol). The reaction was de-gassed again and heated at HO°C in the microwave for 30 minutes. The mixture was concentrated to half its original volume and washed with DCM. The aqueous phase was acidified to pH4 (2M HCl) and resulting precipitate collected, washed with water and dried under vacuum (172mg, 76% Yield). I H NMR (400.0 MHz, DMSO) d 7.41 (2H, br s), 7.69 (2H, m), 7.76 (I H, m), 7.98 (2H, m), 8.09 (2H, m), 8.29 (2H, m), 8.94 (1H, s); MS (ES+) 360.98
Step 5 : 4-(5-amino-6-(5-pheny 1-1 , 2,4-oxadiazol-3-yl)pyrazin-2-yl)pheny I)(1, 4-diazepan-1- yl)methanone
[00372] A solution of 4-[5-amino-6-(5-phenyl-1,2,4-oxadiazol-3-yl)pyrazin-2-yl]benzoic acid (80 mg, 0.2226 mmol), CDI (72.19 mg, 0.4452 mmol), DIPEA (86.31 mg, 1 16.3 μL, 0.6678 mmol), DMAP (2.719 mg, 0.02226 mmol) in DMSO (1.370 mL) was treated with 1,4-diazepane (66.89 mg, 0.6678 mmol) and the resulting solution stirred at RT overnight. An additional equivalent of 1 ,4-diazepane (22.30mg, 0.2226 mmol) was added and the reaction mixture allowed to stir for a further night. The reaction mixture was treated with water and the aqueous layer extracted with EtOAc. The layers were separated and the organics dried (MgSO4), concentrated in vacuo and purified by reverse phase preparative HPLC [Waters Sunfire Cl 8, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min], The fractions were collected and freeze-dried to give the title compound as a yellow solid (58.1mg, 39% Yield). I H NMR (400.0 MHz, DMSO) d 1.96-2.04 (2H, m), 3.25-3.85 (8H, m - with water signal), 7.47 (2H, br s), 7.60 (2H, m), 7.71 (2H, m), 7.79 (1H, m), 8.16 (2H, m), 8.29 (2H, m), 8.77 (2H, m), 8.97 (1H, s); MS (ES+) 442.02
Example 6A : 4-(5-amino-6-(3-phenvlisoxazol-5-vDpvrazin-2-vl)phenv0(1,4-diazepan-1- yl)methanone (Compound IIA-3)
SCHEME
Step 4
Figure imgf000219_0001
Compound IIA-3
Compound IIA-3 was prepared using Method IV-F, Steps 1-6.
METHOD IV-F :
Step 1 : 5-bromo-3-((trimethylsilyl)ethynyl)pyrazin-2-amine
[00373] (Trimethylsilyl)acetylene (1.845 g, 2.655 mL, 18.78 mmol) was added dropwise to a solution of 3,5-dibromopyrazin-2-amine (5 g, 19.77 mmol), triethylamine (10.00 g, -13.77 mL, 98.85 mmol), Copper(I) iodide (451.7 mg, 2.372 mmol) and Pd(PPh3)4 (1.142 g, 0.9885 mmol) in DMF (25.00 mL) and the resulting solution stirred at RT for 30 minutes. The reaction mixture was diluted with EtOAc and water and the layers separated. The aqueous layer was extracted further with EtOAc and the combined organics washed with water, dried (MgSO,)) and concentrated in vacuo. The residue was purified by column chromatography eluting with 15% EtO Ac/Petroleum ether to give the product as a yellow solid (3.99g, 75% Yield). 1H NMR (400.0 MHz, DMSO) d 0.30 (9H, s), 8.06 (1H, s); MS (ES+) 271.82 Step 2 : tert-butyl N-tert-butoxycarbonyl-N-[5-bromo-3-((trimethylsilyl)ethyynyl) pyrazin-2- yl]carbamate
[00374] 5-bromo-3-(2-trimethylsilylethynyl)pyrazin-2-amine (2.85 g, 10.55 mmol) was dissolved in DCM (89.06 mL) and treated with BOC anhydride (6.908 g, 7.272 mL, 31.65 mmol) followed by DMAP (128.9 mg, 1.055 mmol). The reaction was allowed to stir at ambient temperature for 2 hours and then diluted with DCM and NaHCCb and the layers separated. The aqueous layer was extracted further with DCM, dried (MgSO,}), filtered and concentrated in vacuo. The resultant residue was purified by column chromatography eluting with dichloromethane to give the desired product as a colourless oil (4.95g, 99% Yield). I H NMR (400.0 MHz, DMSO) d 0.27 (9H, s), 1.42 (18H, s), 8.50 (I H, s); MS (ES+) 472.09
Step 3 : tert-butyl N-(5-bromo-3-ethynyl-pyrazin-2-yl)-N-tert-butoxycarbonyl-carbamate
[00375] Sodium carbonate (918.5 μL of 2 M, 1.837 mmol) was added to a solution of tert- butyl N-[5-bromo-3-(2-trimethylsilylethynyl)pyrazin-2-yl]-N-tert-butoxycarbonyl-carbamate (720 mg, 1.531 mmol) in DMF (2 mL) and the resulting solution heated at 90°C for 20 minutes. The reaction mixture was allowed to cool to RT and diluted with EtOAc and water and the layers separated. The aqueous layer was extracted further with EtOAc and the combined organics washed with water, dried (MgSO4) and concentrated in vacuo to give the product as a yellow solid (574mg, 94% Yield). 1H NMR (400.0 MHz, DMSO) d 1.43 (18H, s), 3.53 (1H, s), 8.55 (I H, s); MS (ES+) 400.03
Step 4 : tert-butyl N-[5-bromo-3-(3-phenylisoxazol-5-yl)pyrazin-2-yl]-N-tert- butoxycarbonyl-carbamate
[00376] Triethylamine (50.82 mg, 70.00 μL, 0.5022 mmol) was added to a solution of tert-butyl N-(5-bromo-3-ethynyl-pyrazin-2-yl)-N-tert-butoxycarbonyl-carbamate (200 mg, 0.5022 mmol) and N-hydroxybenzimidoyl chloride (78.13 mg, 0.5022 mmol) in THF (16.00 mL) and the reaction mixture stirred at RT for 1 hour. After this time the reaction mixture was heated under reflux for 3 hours, cooled to RT and concentrated in vacuo. The residue was purified by column chromatography eluting with 10% EtOAc/ Petroleum ether to give the product as a colourless oil that crystallised on standing (182mg, 70% Yield). 1H NMR (400.0 MHz, DMSO) d 1.41 (18H, s), 7.37 (1H, s), 7.52 (3H, m), 7.90 (2H, m), 8.68 (1H, s); MS (ES+) 519.05
Step 5 : 4-(5-(bis(tert-butoxycarbonyl)amino)-6-(3-phenylisoxazol-5-yl)pyrazin-2- yl)benzoic acid
[00377] Tert-butyl N-[5-brorno-3-(3-phenylisoxazol-5-yl)pyrazin-2-yl]-N-tert- butoxycarbonyi-carbamate (184 mg, 0.3379 mmol), 4-boronobenzoic acid (56.07 mg, 0.3379 mmol) and Na2CO3 (71.63 mg, 0.6758 mmol) were suspended in MeCN (2.896 mL) / water (2.896 mL) and the mixture de-gassed (x5) and treated with Pd(PPh3)4 (39.05 mg, 0.03379 mmol). The reaction was de-gassed again and heated at 1 1O°C in the microwave for 30 minutes. The reaction mixture was concentrated to half its original volume and washed with DCM. The aqueous phase was acidified to pH4 by (2M HCl) and resulting precipitate collected, washed with water and dried under vacuum (120mg, 99% Yield). MS (ES+) 359.12
Step 6 : 4-(5-amino-6-(3-phenylisoxazol-5-yl)pyrazin-2-yl)phenyl)(1,4-diazepan-1- yl)methanone
[00378] To a solution of 4-[5-amino-6-(3-phenylisoxazol-5-yl)pyrazin-2-yl]benzoic acid (120 mg, 0.3349 mmol), CDI (108.6 mg, 0.6698 mmol), DIPEA (129.9 mg, 175.1 μL, 1.005 mmol), DMAP (4.091 mg, 0.03349 mmol) in DMSO (2.054 mL) was added tert-butyl 1,4- diazepane-1-carboxylate (201.3 mg, 1.005 mmol) and the resulting solution stirred at RT for 3 hours. After this time water was added and the aqueous layer extracted with EtOAc, and the combined organics dried (MgSO4) and concentrated in vacuo. The resultant residue was taken up in DCM (3.000 mL) and treated with TFA (763.7 mg, 516.0 μL, 6.698 mmol) and the mixture stirred overnight at RT. The mixture was concentrated in vacuo and the residue taken up in dichloromethane (5 mL) and washed with NaHCθ3 aqueous solution. The organic layer was dried (MgSO4), concentrated in vacuo and purified by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. The fractions were collected and freeze-dried to give the title compound as a yellow solid (68.7mg, 37% Yield). I H NMR (400.0 MHz, DMSO) d 1.95 (2H, m), 3.25-3.96 (8H, m partially hidden by water peak), 7.08 (2H, br s), 7.54-7.61 (5H, m), 7.78 (1H, s), 8.03-8.05 (2H, m), 8.19 (2H, m), 8.72 (2H, br s), 8.89 (1H, s); MS (ES+) 441.21
ExamEle_7j_5-(pyridin-3-yl)-3-(5-(thiophen-2-yl)-4H-1,2,4-triazol-3-yI)pyrazin-2-amine (Compound IIIA-4)
SCHEME
Step 4
Figure imgf000222_0001
Compound IIIA-4
Compound IIIA-4 was prepared using Method IV-G, Steps 1-5.
METHOD IV-G :
Step 1 : Methyl 3-amino-6-bromopyrazine-2-carboxylate
[00379] A mixture of methyl 3-aminopyrazine-2-carboxy!ate (8.35 g, 54.53 mmol) and N- bromo-succinimide (9.705 g, 54.53 mmol) were stirred in MeCN (100 mL) at room temp for 16 hours. The resultant precipitate was filtered, washed with MeCN and dried under vacuum to give the desired product as a yellow solid (11.68 g, 92%). 1H NMR (400.0 MHz, DMSO) d 3.85 (3H, s), 7.55 (2H, br s), 8.42 (1H, s); MS (ES+) 233.78
Step 2 : 3-amino-6-(pyridin-3-yl)pyrazine-2-carboxylic acid [00380] A mixture of methyl S-amino-ό-bromo-pyrazine-Z-carboxylate (8 g, 34.48 mmol), diethyl-(3-pyridyl)borane (6.084 g, 41.38 mmol), dichloropalladium; triphenylphosphane (1.21O g, 1.724 mmol), disodium carbonate (51.70 mL of 2 M, 103.4 mmol) in DME (100 mL) were heated at 80°C for 16 hours. The reaction mixture was cooled and treated with EtOAc and the resultant precipitate was isolated by filtration. Water was added to the solid and then the suspension heated and filtered hot. The solution was then allowed to cool and then acidified (AcOH) to approx pH 5. The precipitate was collected and washed with MeOH and dried under vacuum (6.23g, 84% Yield). 1H NMR (400.0 MHz, DMSO) d 7.47 (1H, m), 7.60 (2H, br s), 8.42 - 8.57 (2H, m), 8.97 (1H, s), 9.26 (1H, m); MS (ES+) 216.89
Step 3 : Methyl 3-amino-6-(pyridin-3-yl)pyrazine-2-carboxylate
[00381] To 3-amino-6-(3-pyridyl)pyrazine-2-carboxylic acid (2 g, 9.251 mmol) in MeOH (50 mL) was added cone. H2SO4 (907.3 mg, 493.1 μL, 9.251 mmol) and the mixture heated to reflux for 2 hours. The solvent was removed under vacuum and the mixture neutralised with aqueous Na2CO3 and the resulting solid collected by filtration and dried to give the desired product (2.08 g, 97% Yield). MS (ES+) 231.87
Step 4 : 3-amino-6-(pyridin-3-yl)pyrazine-2-carbohydrazide
[00382] Methyl 3-amino-6-(3-pyridyl)pyrazine-2-carboxylate (2 g, 8.687 mmol) was heated in hydrazine (1.392 g, 1.363 mL, 43.43 mmol) with a minimal amount of MeOH (5 mL) added at 80°C for 2 hours. The reaction was treated with water and the product collected by filtration, washed with methanol and dried to give the desired product as a brown solid (1.17 g, 58% Yield). I H NMR (400.0 MHz, DMSO) d 7.43 (1H, m), 7.47 (2H, br s), 8.54 (2H, m), 8.90 (1H, s), 9.38 (1H, m), 10.16 (I H, br s); MS (ES+) 231.96
Step 5 : 5-(pyridin-3-yl)-3-(5-(thiophen-2-yl)-4//-l ,2,4-triazoI-3-yl)pyrazin-2-amine
[00383] 3-amino-6-(3-pyridyl)pyrazine-2-carbohydrazide (40 mg, 0.173 mmoles), thiophene-2-carboxamidine (21.92mg, 0.173 mmoles) and sodium ethanolate (1 1.82mg, 0.173 mmoles) were added to a microwave vial. DMF (1 mL) was then added and the vial sealed and heated in the microwave at 160°C for 40 minutes. The reaction mixture was filtered and purified by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. The fractions were collected and freeze-dried to give the title compound (23.4mg, 31% Yield). 1H NMR (400.0 MHz, DMSO-d6) d 14.96 (s, 1H), 9.55 (s, I H), 8.99 (s, 1H), 8.84 (d, J = 6.1 Hz, 1H), 8.69 (dd, J = 1.2, 4.9 Hz, 1H), 7.95 (s, 2H), 7.81 (d, J = 3.0 Hz, I H), 7.73 - 7.68 (m, 2H) and 7.22 (dd, J = 3.8, 4.8 Hz, I H) ppm; MS (ES+) 323.10
E2camEle_8A_2.N-(2-(5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- yl)phenyl)ethanamide (Compound IA-267)
Figure imgf000224_0001
Compound IA-267
Compound IA-267 was prepared using Method IV-A, Steps 1-2, followed by Method IV-H, Step 1.
METHOD II-H: Step 1: N-(2-(5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- yl)phenyl)ethanamide
[00384] A solution of 5-bromo-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-amine (100 mg, 0.31 mmol) , 2-ethanamidophenylboronic acid (56.25 mg, 0.31 mmol), tetrakis(triphenylphosphine)palladium (18.17 mg, 0.015 mmol) and Νa2CC>3 (471 μL, 2M aqueous solution) were added to a 10 mL microwave vial. Dioxane (3 mL) was then added and the vial sealed. The reaction mixture was heated in the microwave at 150 °C for 30 min. After this time methanol was added and the reaction mixture filtered. The solid was then washed with water (5 mL) and MeOH (5 mL) and dried under vacuum to give the product (31.0 mg, 28 % yield); 1 H NMR (400.0 MHz, DMSO) d 2.04 (s, 3H), 7.26 (t, 1 H), 7.44 - 7.40 (m, 1 H), 7.69 - 7.67 (m, 4H), 7.80 (d, 2H), 8.15 - 8.13 (m, 3H), 8.73 (s, 1 H) and 10.76 (s, 1H) ppm; MS (ES+) 373.0
[00385] The following compounds were all prepared using the method described for Compound IA-267 above.
Compound IA-75 1H NMR (400.0 MHz, DMSO) 3.25 (s, 3H), 7.63 (s, 1H), 7.63 (dd, 2H), 7.74 (t, 3H), 7.90 (dd, 1H), 8.09 (dd, 2H), 8.40 (dd, 1H), 8.54 (t, 1H) and 9.00 (s, 1H) ppm; MS (ES+) 394.0
Compound IA-89 5-(4-methylsulfonylphenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- amine; MS (ES+) 393.0
Compound IA-93 5-(l-naphthyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-amine; MS (ES+) 365.0
Compound IA-94 5-(2-(dimethylamino)phenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin- 2-amine; MS (ES+) 359.0
Compound IA-96 3-(5-phenyl-1,3,4-oxadiazol-2-yl)-5-[3-(trifluoromethyl)phenyl]pyrazin- 2-amine; MS (ES+) 384.0
Compound IA-100 3-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]benzamide; MS (ES+) 359.0
Compound IA-104 3-(5-phenyl-l ,3,4-oxadiazol-2-yl)-5-(3-thienyl)pyrazin-2-amine; MS (ES+) 322.0
Compound IA-105 methyl 2-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- yl]benzoate; 1H NMR (400.0 MHz, DMSO) ^ 3.61 (s, 3H), 7.56 (m, I H), 7.65-7.72 (m, 7H), 7.87 (d, 1 H), 8.16 (m, 2H) and 8.72 (s, 1 H) ppm; MS (ES+) 374.0
Compound IA-110 l-[4-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- yl]phenyl]ethanone; MS (ES+) 358.0 Compound IA-116 5-(4-isopropylsulfonylphenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2- yl)pyrazin-2-amine; 1H NMR (400.0 MHz, DMSO) d 1.20 (d, 6H), 3.47 (t, 1H), 7.66-7.72 (m, 3H), 7.98 (d, 4H), 8.17-8.19 (m, 2H), 8.40 (dd, 2H), and 9.60 (s, 1H) ppm; MS (ES+) 422.0
Compound IA-118 5-(2-((dimethylamino)methyl)phenyl)-3-(5-phenyl-1,3,4-oxadiazol-2- yl)pyrazin-2-amine; MS (ES+) 373.0
Compound IA-125 5-[2-(methoxymethyl)phenyl]-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin- 2-amine; MS (ES+) 360.0
Compound IA-137 2-[2-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- yl]phenyl]ethanol; 1H NMR (400.0 MHz, DMSO) d 2.89 (t, 2H), 3.74 (s, 2H), 4.61 (s, 1H), 7.32-7.43 (m, 3H), 7.47-7.49 (m, I H), 7.61-7.69 (m, 5H), 8.13-8.16 (m, 2H) and 8.49 (s, 1H) ppm; MS (ES+) 360.0
Compound IA-141 5-(4-pyridyl)-3-[5-(2-thienyl)-1,3,4-oxadiazol-2-yI]pyrazin-2-amine; 1H NMR (400.0 MHz, DMSO) d 7.22 (t, 1H), 7.38 (t, 1H), 7.80-7.82 (m, 1H), 8.04-8.09 (m, 4H), 8.70 (dd, 2H) and 9.08 (s, 1H) ppm; MS (ES+) 323.1
Compound IA-144 N-[3-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- yl]phenyl]methanesulfonamide; MS (ES+) 408.0
Compound IA-149 5-(4-ethylsulfonylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- amine; MS (ES+) 408.0
Compound IA-150 3-(5-phenyl-1,3,4-oxadiazol-2-yl)-5-[3-(trifluoromethoxy)phenyl] pyrazin-2-amine; MS (ES+) 400.0
Compound IA-169 5-[4-(2-dimethylaminoethylsuIfonyl)phenyl]-3-(5-phenyl- 1,3,4- oxadiazol-2-yl)pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 2.81 (s, 6H), 3.43-3.40 (m, 2H), 3.89-3.93 (m, 2H), 7.68-7.73 (m, 3H), 7.90 (br s, 2H), 8.07 (d, 2H), 8.17-8.19 (m, 2H), 8.45 (d, 2H) and 9.10 (s, 1 H) ppm; MS (ES+) 451.0 Compound IA-170 5-(3-furyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-amine; MS (ES+) 306.0
Compound IA-174 3-(5-phenyl-l ,3,4-oxadiazol-2-yl)-5-[2(-trifluoromethyl)phenyl]pyrazin- 2-amine; MS (ES+) 384.0
Compound IA-176 5-(2-bromophenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-amine; MS (ES+) 393.0
Compound IA-182 5-(m-tolyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-amine; MS (ES+) 330.0
Compound IA-190 5-(2-methylsulfonylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- amine; MS (ES+) 394.0
Compound IA-197 3-(5-phenyl-l ,3,4-oxadiazol-2-yl)-5-[4-(4-piperidylsulfonyl)phenyl] pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.76-1.70 (m, 2H), 2.08 (d, 2H), 2.89 (d, 2H), 3.37 (d, 2H), 3.66 (d, 1H), 7.70 (d, 2H), 7.82 (s, 1H), 7.86 (s, 1H), 7.98 (d, 2H), 8.13 (s, I H), 8.18 (d, 2H), 8.44 (d, 2H), 8.63 (s, I H) and 9.08 (s, I H) ppm; MS (ES+) 463.0
Compound IA-202 [3-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- yl]phenyl]methanol; MS (ES+) 346.0
Compound IA-210 5-(l-ethylpyrazol-4-yl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- amine; MS (ES+) 334.0
Compound IA-216 3-(5-phenyl-l ,3,4-oxadiazol-2-yl)-5-(8-quinolyl)pyrazin-2-amine; 1H NMR (400.0 MHz, DMSO) d 7.63-7.71 (m, 4H), 7.82 (t, 3H), 8.10-8.15 (m, 3H), 8.26 (m, 1H), 8.53 (m, I H), 9.01 (dd, 1H) and 9.14 (s, I H) ppm; MS (ES+) 366.023
Compound IA-218 4-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl]benzamide; MS (ES+) 359.0 Compound IA-221 2-[2-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- yl]phenyl]acetonitrile; MS (ES+) 355.0
Compound IA-230 5-(2-methylsulfanylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- amine; MS (ES+) 362.0
Compound IA-241 5-(2-methylsulfinylphenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- amine; MS (ES+) 378.0
Compound IA-244 2-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N,N- dimethyl-benzamide; MS (ES+) 387.0
Compound IA-247 N-[4-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- yl]phenyl]acetamide; MS (ES+) 373.0
Compound IA-249 l-[3-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- yl]phenyl]ethanone; 1H NMR (400.0 MHz, DMSO) d 2.70 (s, 3H), 1.61-l.lλ (m, 4H), 8.00- 8.02 (m, 1H), 8.17 (dd, 2H), 8.39 (d, 1H), 8.64 (d, I H) and 9.05 (s, 1H) ppm; MS (ES+) 358.0
Compound IA-252 3-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]benzonitrile; MS (ES+) 341.0
Compound IA-253 3-(5-phenyl-1,3,4-oxadiazol-2-yl)-5-(2-vinylphenyl)pyrazin-2-amine; MS (ES+) 342.0
Compound IA-259 5-(benzothiophen-7-yl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- amine; MS (ES+) 371.0
Compound IA-260 3-(5-phenyl-l ,3,4-oxadiazol-2-yl)-5-(5-quinolyl)pyrazin-2-amine; MS (ES+) 366.0
Compound IA-266 2-[2-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- yl]phenyl]acetamide; 1H NMR (400.0 MHz, DMSO) d 3.64 (s, 2H), 6.88 (s, 1H), 7.40 (dd, 4H), 7.51-7.53 (m, 1H), 7.62 (s, 1H), 7.67 (dd, 4H), 8.12 (d, 2H) and 8.49 (s, 1H) ppm; MS (ES+) 373.0
Compound IA-271 '3-(5-phenyl-] ,3,4-oxadiazol-2-yl)-5-(2-piperazin-1-yl-4-pyridyl)pyrazin- 2-amine; MS (ES+) 400.0
Compound IA-274 5-(4-methylsulfinylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- amine 1H NMR (400.0 MHz, DMSO) d 2.81 (s, 3H), 7.69 (d, 3H), 7.83 (d, 3H), 8.16-8.19 (m, 2H), 8.30-8.33 (m, 2H) and 9.02 (s, 1H) ppm; MS (ES+) 377.0
Examβle_9A_j_4-[5-amino-6-[5-(2-chloroanilino)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N- dimethyl-benzamide (Compound IA-151)
SCHEME
Figure imgf000229_0001
Compound IA-151
Compound IA-151 was prepared using Method IV-C, Steps 1-2, followed by Method IV-I, Step 1.
METHOD IV-I
Step 1: 4-[5-amino-6-[5-(2-chloroanilino)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N-dimethyl- benzamide
[00386] A solution of 2-chloroaniline (31.85 mg, 41.15 μL, 0.2497 mmol) in dichloromethane (2 mL) was slowly added dropwise to a solution of 1 ,1'- thiocarbonyldiimidazole (53.39 mg, 0.2996 mmol) in dichloromethane (1.5 mL) and and the resulting solution stirred at room temperature for Ih. Additional 1,1'- thiocarbonyldiimidazole (8.9 mg, 0.05 mmol) was added, and the reaction mixture stirred at room temperature overnight. The reaction mixture diluted with water and extracted with dichloromethane (3 x 5 mL). The organic extracts were dried over MgSO4, filtered and evaporated to dryness to leave a yellow solid. The solid was re-dissolved in dichloromethane (1.5 mL) and 4-(5-amino-6-(hydrazinecarbonyl)pyrazin-2-yl)-N,N-dimethylbenzamide (75 mg, 0.2497 mmol) added and the reaction mixture stirred at room temperature for 48 h. The reaction mixture was evaporated to drymess and then triturated with EtOAc/Petrol/Ether to give a yellow solid, 4-[5-amino-6-[[(2 chlorophenyl)carbamothioylamino]carbamoyl] pyrazin-2-yl]-N,N-dimethyl-benzamide. This was re-dissolved in dichloromethane (1.5 mL) and EDC (71.81 mg, 0.3746 mmol) added and the resulting solution heated at 40 °C for 3 h. The reaction mixture was cooled to room temperature and concentrated in vacuo and the solid triturated with EtOAc and petroleum ether to yield the product as a yellow solid (28.9 mg, 26 % yield); 1 H NMR (400.0 MHz, DMSO) d 2.96 (s, 3H), 3.01 (s, 3H), 7.15-7.25 (m, 1H), 7.40-7.49 (m, I H), 7.53 (d, 3H), 7.70 (br s, 2H), 8.1 1 (d, 3H), 8.89 (s, 1H) and 10.45 (s, 1H) ppm; MS (ES+) 436.1 1
Example IQA : 4-r5-amino-6-r5-('p-tolvl')-1.3.4-oxadiazol-2-vllDvrazin-2-vll-N,N-dimethvl- benzamide (Compound IA-263)
SCHEME
Figure imgf000230_0001
Compound IA-263
Compound IA-263 was prepared using Method IV-B, Steps 1-2, followed by Method IV-J, Step 1.
METHOD IV-J
Step 1: 4-[5-amino-6-[5-(p-tolyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N-dimethyl- benzamide [00387] A solution of 4-[5-amino-6-[[(4-methylbenzoyl)amino]carbamoyl]pyrazin-2-yl]- N,N-dimethyl-benzamide (90 mg, 0.2151 mmol) and POCl3 (3.298 g, 2.005 mL, 21.51 mmol) was heated at 110 °C for 2h. After this time the reaction mixture was cooled to room temperature and ice added. Once all of the ice had melted the reaction mixture was extracted with dichloromethane (3 x 5 mL) and the combined organics dried over MgSθ4 and concentrated in vacuo. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. The product fractions combined and lyophilised to leave the product as a yellow solid (21.2 mg, 20 % yield); 1H NMR (400.0 MHz, DMSO) d 2.22 (s, 3H), 2.75 (m, 6H), 7.26 (m, 2H), 7.32 (m, 2H), 7.58 (br s, 2H), 7.83 (m, 2H), 7.95 (m, 2H) and 8.77 (1H, s); MS (ES+) 401.15
The following compounds were all prepared using the method described for Compound IA-263 above.
Compound IA-135 4-[5-amino-6-[5-(l -methylpyrrol-2-yl)-l ,3,4-oxadiazol-2-yl]pyrazin-2- yl]-N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.97 (m, 6H), 4.06 (s, 3H), 6.30 (m, I H), 7.03 (m, I H), 7.26 (m, 1H), 7.53-7.55 (m, 2H), 7.77 (br s, 2H), 8.15 (m, 2H) and 8.97 (1H, s) ppm; MS (ES+) 390.14
Example HA : 4-r5-amino-6-F5-(azetidin-1-viyi.3.4-oxadiazol-2-vHpyrazin-2-yl1-N,N- dimethyl-benzamide (Compound IA-192)
SCHEME Method IV-C IV-K
Figure imgf000231_0001
Compound IA-192
Compound IA-192 was prepared using Method IV-C, Steps 1-2, followed by Method IV-K, Steps 1-2. METHOD IV-K
Step 1: 4-(5-amino-6-(5-oxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)pyrazin-2-yl)-N,N- dimethylbenzamide
[00388] DIPEA (86.08 mg, 116.0 μL, 0.6660 mmol) was added to a solution of 4-(5- amino-6-(hydrazinecarbonyl)pyrazin-2-yl)-Ν,Ν-dimethylbenzamide (100 mg, 0.3330 mmol) in DCM (6.500 mL) under nitrogen. A solution of triphosgene (39.53 mg, 0.1332 mmol) in DCM (100.0 μL) was then added dropwise to the stirred solution. The reaction mixture was stirred at room temperature for 2h. The reaction mixture was filtered and the solid obtained dried under vacuum to yield the product (106.g mg, 98% yield); 1 H NMR (400.0 MHz, DMSO) d 2.96 (s, 3H), 3.00 (s, 3H), 7.33 (br s, 2H), 7.52 (d, 2H), 8.09 (d, 2H), 8.89 (s, 1H) and 12.98 (br s, 1 H) ppm; MS (ES+) 327.12
Step 2: 4-[5-amino-6-[5-(azetidin-1-yl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N-dimethyl- benzamide
[00389] DIPEA (38.44 mg, 51.81 μL, 0.2974 mmol), azetidine (8.490 mg, 0.1487 mmol) and bromo(tripyrrolidin-l -yl)phosphonium hexafluorophosphate (76.27 mg, 0.1636 mmol) were added to a solution of 4-(5-amino-6-(5-oxo-4,5-dihydro-1,3,4-oxadiazol-2-yl)pyrazin-2- yl)-N,N-dimethylbenzamide (50 mg, 0.1487 mmol) in DMF (485.1 μL) and the resulting solution stirred at room temperature for 2h. The reaction mixture was filtered and purified by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% -
95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min].
Product fractions were concentrated in vacuo and triturated with dichloromethane/ diethyl ether to give the product (8.6 mg, 15 % yield); 1H NMR (400.0 MHz, DMSO) d 2.96 (s, 3H),
3.00 (s, 6H), 4.25 (t, 4H), 7.52 (dd, 2H), 7.65 (br s, 1H), 8.04-8.06 (m, 2H) and 8.83 (s, 1H) ppm; MS (ES+) 366.21
[00390] The following compounds were all prepared using the method described for
Compound IA-192 above.
Compound IA-250 4-[5-amino-6-[5-(N-methylanilino)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-
N,N-dimethyl-benzamide; MS (ES+) 416.18 Example 12A : 4-r5-amino-6-r5-(2-furvl)-1,3,4-oxadiazol-2-vllpvrazin-2-vll-N.N-dimethvl- benzamide (Compound IA-115)
SCHEME
Figure imgf000233_0001
Compound IA-11S
Compound IA-1 15 was prepared using Method IV-B, Steps 1-2, followed by Method IV-L, Step 1.
METHOD IV-L
Step 1: 4-[5-amino-6-[5-(2-furyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N-dimethyl- benzamide
[00391] Dibromo(triphenyl)phosphorane was added to a solution of 3-amino-6-[4- (dimethylcarbamoyl)phenyl]pyrazine-2-carboxylic acid (100 mg, 0.35 mmol) and furan 2- carbohydrazide (44.1 mg, 0.35 mmol) in acetonitrile (3.0 mL) at room temperature. Bright yellow solution observed. The resulting solution was stirred at room temperature for 30 min. After this time, DIPEA (304 μL, 1.75 mmol) was added dropwise and the reaction mixture stirred at room temperature for 30 min. The reaction mixture was filtered to leave a yellow solid. The solid was purified by reverse phase preparative HPLC [Waters Sunfire Cl 8, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were freeze dried to give the product as a yellow solid (67.6 mg, 51% yield); 1H NMR (400.0 MHz, DMSO) d 2.97 (m, 6H), 6.87 (m, 1H), 7.54-7.56 (m, 3H), 7.57 (br s, 2H), 8.15 (m, 3H) and 8.98 (1H, s) ppm; MS (ES+) 377.17
[00392] The following compounds were all prepared using a method similar to the one described for Compound IA-1 15 above. Compound IA-71 4-[5-amino-6-[5-(o-tolyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N- dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.74 (s, 3H), 2.98 (m, 6H), 7.48-7.62
(m, 5H), 7.80 (br s, 2H), 8.07 (m, I H), 8.15 (m, 2H) and 8.99 (1H, s) ppm; MS (ES+) 401.21
Compound IA-87 4-[5-amino-6-[5-(4-hydroxyphenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-
N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.98 (m, 6H), 7.04 (m, 2H), 7.54
(m, 2H), 7.76 (br s, 2H), 8.01 (m, 2H), 8.16 (m, 2H), 8.97 (s, 1H) and 10.42 (s, 1H) ppm; MS
(ES+) 403.16
Compound IA-IOl 4-[5-amino-6-(5-cyclopropyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N,N- dimethyl-benzamide I H NMR (400.0 MHz, DMSO) d 1.14- 1.18 (m, 2H), 1.22- 1.25 (m,
2H), 2.40 (m, I H), 3.01 (m, 6H), 7.54 (m, 2H), 7.68 (br s, 2H), 8.10 (m, 2H) and 8.93 (s, I H) ppm; MS (ES+) 351.17
Compound IA-157 4-[5-amino-6-[5-(4-methoxyphenyl)-1,3,4-oxadiazol-2-yI]pyrazin-2-yl]-
N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.98 (m, 6H), 3.89 (s, 3H), 7.22
(m, 2H), 7.54 (m, 2H), 7.76 (br s, 2H), 8.10 (m, 2H), 8.16 (m, 2H) and 8.98 (s, 1H) ppm; MS
(ES+) 417.18
Compound IA-167 4-[5-amino-6-[5-(3-methyl-2-thienyl)-l,'3,4-oxadiazol-2-yl]pyrazin-2- yl]-N,N-dimethyl-benzamide MS (ES+) 407.18
Compound IA-205 4-[5-amino-6-[5-(2-iodophenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-
N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.96 (m, 6H), 7.43 (m, I H), 7.52
(m, 2H), 7.69 (m, I H), 7.81 (br s, 2H), 7.96 (m, 1H), 8.16 (m, 2H), 8.20 (m, 1H) and 9.01 (s,
1H) ppm; MS (ES+) 513.01
Compound IA-237 4-[5-amino-6-[5-(m-tolyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N- dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.53 (s, 3H), 3.05 (m, 6H), 7.57-7.65
(m, 4H), 7.84 (br s, 2H), 8.04 (m, 2H), 8.23 (m, 2H) and 9.05 (s, 1H) ppm; MS (ES+) 401.2
Compound IA-242 4-[5-amino-6-[5-(2-methoxyphenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yI]-
N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.97 (m, 6H), 3.97 (s, 3H), 7.19-
7.23 (dt, I H) 7.35 (m, 1H), 7.56 (m, 2H), 7.65-7.70 (m, I H), 7.77 (br s, 2H), 7.99 (dd, I H),
8.14 (m, 2H) and 8.98 (s, I H) ppm; MS (ES+) 417.19
Compound IA-245 4-[5-amino-6-[5-(5-methyl-2-thienyl)-1,3,4-oxadiazol-2-yl]pyrazin-2- yl]-N,N-dimethyl-benzamide 1 H NMR (400.0 MHz, DMSO) d 2.60 (s, 3H), 2.98 (m, 6H), 7.08 (d, 1H), 7.53 (m, 2H), 7.74 (br s, 2H), 7.82 (m, 1H), 8.15 (m, 2H) and 8.97 (1H, s) ppm; MS (ES+) 407.12
Compound IA-262 4-[5-amino-6-[5-(3-thienyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N- dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.98 (m, 6H), 7.53 (m, 2H), 7.75-7.77 (m, 3H), 7.89 (m, 1H), 8.17 (m, 2H), 8.57 (m, 1H) and 8.98 (s, 1H) ppm; MS (ES+) 393.12
Example 13A: 4-r5-amino-6-f5-Dhenyl-1.3.4-oxadiazol-2-v0pvrazin-2-vn-3- (difluoromethyl)-N,N-dimethyl-benzamide (Compound IA-126)
SCHEME
Method IV-A Method IV-M Steps 1-3 Step i
Figure imgf000235_0001
Compound IA-126
Compound IA-126 was prepared using Method IV-A, Steps 1-3, followed by Method IV-M, Step 1.
METHOD IV-M
Step 1: 4-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl]-3-(difluoromethyl)-N,N- dimethyl-benzamide
[00393] LiOH (495.9 μL of 1 M aq solution, 0.4959 mmol) was added to a suspension of methyl 4-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-3- (difluoromethyl)benzoate (70 mg, 0.1653 mmol) in THF (5 mL) and methanol (2 mL). The reaction mixture was stirred for 4h at room temperature and then concentrated in vacuo. The residue was acidified to pH2 by the addition of IM HCl. A precipitate formed which was then filtered and washed with water, ethylacetate and ether. The solid was taken up in DMF (2 mL) and TBTU (79.63 mg, 0.2480 mmol) and DIPEA (64.09 mg, 86.37 μL, 0.4959 mmol) added followed by N-methylmethanamine (495.9 μL of 1 M, 0.4959 mmol) in THF. The resulting mixture was stirred at room temperarature for 2h, diluted with ethylacetate (5 mL), washed with water (2 x 5 mL) and concentrated in vacuo. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were freeze dried to leave the product as a solid (29.6 mg, 40% yield); 1H NMR (400.0 MHz, DMSO) d 3.0 (d, 6H), 7.6-7.7 (m, 4H), 7.82 (s, 1H), 7.9 (d, 2H), 8.15- 8.18 (m, 2H) and 8.7 (s, 1H) ppm; MS (ES+) 437.2
[00394] The following compounds were all prepared using a method similar to the one described for Compound IA-126 above.
Compound IA-148 4-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-3-(2- fluorovinyl)-N,N-dimethyl-benzamide I H NMR (400.0 MHz, DMSO) d 3.05 (d, 6H), 6.15 (dd, 0.5H), 6.85-6.95 (m, 1 H), 7.1 (d, 0.25H), 7.45-7.55 (m, 2H), 7.6-7.7 (m, 5H), 7.8 (br s, 2H), 8.1 -8.15 (m, 2H) and 8.45-8.48 (m, 1 H) ppm; MS (ES+) 431.2 Compound IA-161 4-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N,N- dimethyl-3-oxazol-5-yl-benzamide 1H NMR (400.0 MHz, DMSO) d 3.02 (d, 6H), 7.6-7.75 (m, 4H), 7.75-7.8 (m, 3H), 8.1 (d, 2H), 8.19 (s, 1H) and 8.19 (s, 1H) ppm; MS (ES+) 454.1 Example 14A: 5-(2-ethynylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-amin (Compound IA-194)
SCHEME
Method IV-A
Figure imgf000236_0001
Compound IA-194 Compound IA-194 was prepared using Method IV-A, Steps 1-2, followed by Method IV-N, Steps 1-3.
METHOD IV-N
Step 1: di-tert-butyl 5-bromo-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- yliminodicarbonate
[00395] 5-bromo-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-amine (4 g, 12.57 mmol) was suspended in DCM (59.76 mL) and THF (59.76 mL) and DMAP (153.6 mg, 1.257 mmol) was added,. Di-tert-butyl dicarbonate (8.230 g, 8.663 mL, 37.71 mmol) was addded in portions and the reaction allowed to stir at room temperature overnight. The reaction mixture was concentrated under reduced pressure and purified by column chromatography on silica gel eluting 10-20%EtAc/petrol to give the product as a cream coloured solid (5.72g, 88% yield); 1H NMR (400.0 MHz, DMSO) d 1.29 (s, 18H), 7.69 (d, 3H), 8.13 (d, 2H) and 9.17 (s,
1 H) ppm
Step 2: tert butyl-5-(2-bromophenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl(tert- butoxycarbonyl)carbamate
[00396] A mixture of (2-bromophenyl)boronic acid (100 mg, 0.4979 mmol), tert-butyl N- [5-bromo-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N-tert-butoxycarbonyl-carbamate (258.1 mg, 0.4979 mmol), potassium carbonate (206.5 mg, 1.494 mmol) and triphenylphosphane palladium (13.06 mg, 1 1.54 μL, 0.04979 mmol) in DMF (3 mL) was heated at 5O°C for Ih. The reaction mixture was cooled to room temperature and filtered through a Celite,™ pad. The pad was washed with ethyl acetate (1 x 10 mL) and the combined filtrates were washed with water (2 x 10 mL) and brine (1 x 10 mL), dried over MgSO4 and concentrated to leave the product as a solid which was used directly in the next step without further purification.
Step 3: 5-(2-ethynylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-amin
[00397] A suspension of tert-butyl N-[5-(2-bromophenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2- yl)pyrazin-2-yl]-N-tert-butoxycarbonyl-carbamate (100 mg, 0.1682 mmol), copper iodided (9.612 mg, 0.05047 mmol), dichloropalladium; triphenylphosphane (35.42 mg, 0.05046 mmol), triethylamine (21 1.0 μL, 1.514 mmol) and ethyny!(trimethyl)silane (85.58 μL, 0.6056 mmol) were heated at 6O°C in toluene (10 mL) for I h. The reaction mixture was cooled to room temperature and filtered through a Celite™ pad and the filtrate concentrated in vacuo to leave an oil. This was purified by column chromatography on silica eluting with diethyl ether/ petroleum ether as eluent to yield the product as a yellow oil. This oil was dissolved in THF (2 mL) followed by the addition of tetrabutylammonium fluoride (336.4 μL of 1 M, 0.3364 mmol) and the resulting solution stirred at room temperature for Ih. The mixture was diluted with ethylacetate (5 mL), washed with water and brine and concentrated in vacuo to leave a solid. The solid was dissolved in dichloromethane (10 mL) and trifluoroacetic acid (19.18 mg, 12.96 μL, 0.1682 mmol) was added. The resulting mixture was stirred at room temperature for Ih and then concentrated in vacuo to leave an oil which was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were freeze dried to leave the product as a solid (12.0 mg, 24% yield); 1H NMR (400.0 MHz, MeOD) d 2.77 (s, I H), 6.42-6.5 (m, I H), 6.55- 6.62 (m, I H), 6.6-6.75 (m, 4H), 6.82 (d, 1H), 7.3 (d, 1H) and 7.83 (s, I H) ppm; MS (ES+) 340.1
Example 15A: 4-(5-amino-6-(5-(2-vinylphenyl)-1,3,4-oxadiazol-2-yl')pyrazin-2-yD-NJV- dimethylbenzamide (Compound IA-77)
SCHEME
Figure imgf000238_0001
Compound IA-77
Compound IA-77 was prepared using Method IV-B, Steps 1 -2, followed by Method IV-L, Step 1 , followed by Method IV-O, Step 1. METHOD IV-O
Step 1: 4-(5-amino-6-(5-(2-vinylphenyl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl)-N,N- dimethylbenzamide
[00398] A solution of 4-[5-amino-6-[5-(2-iodophenyl)-l ,3,4-oxadiazoI-2-yl]pyrazin-2-yl]- Ν,Ν-dimethyl-benzamide (100 mg, 0.1952 mmol), potassium trifluoro-vinyl-boron (31.37 tng, 0.2342 mmol), triethyl amine (81.63 μL, 0.5857 mmol) and cyclopenta-l ,4-dien-1- yl(diphenyl)phosphane; dichloromethane; dichloropalladium; iron (31.88 mg, 0.03904 mmol) in propanol (2.000 mL) was degassed and flushed with nitrogen (3x) and the resulting solution heated at 100 °C for 1 h. The reaction mixture cooled to room temperature and concentrated in vacuo. The residue was purified by column chromatography on silica eluting with ethyl acetate. Product fractions were combined and concentrated in vacuo to leave the product as a yellow solid (43.1 mg, 53 % yield); 1H NMR (400.0 MHz, DMSO) d 2.97 (m, 6H), 5.51 (m, 1H), 5.98 (m, 1H), 7.54 (m, 2H), 7.58-7.70 (m, 3H), 7.78 (br s, 2H), 7.89 (m, 1H), 8.06 (m, 2H) and 9.00 (s, 1H) ppm; MS (ES+) 413.15
Example 16A: 2-(5-amino-6-(5-(thiophen-2-vl)-h3,4-oxadiazol-2-yl)pyrazin-2-yl)-5-(l ,4- diazepane-1-carbonyl)benzonitrile (Compound IA-152)
SCHEME
Figure imgf000239_0001
Compound IA- 152 was prepared using Method IV-P, Steps 1-2.
METHOD IV-P
Step 1: 5-bromo-3-(5-(thiophen-2-yl)-1,3,4-oxadiazol-2-yl)pyrazin-2-amine [00399] 3-amino-6-bromo-pyrazine-2-carboxylic acid (3.2 g, 14.68 mmol) and thiophene- 2-carbohydrazide (2.152 g, 14.68 mmol) were suspended in acetonitrile (48.00 mL) at room temperature and dibromo(triphenyl)phosphorane (24.79 g, 58.72 mmol) was added, followed byadditional acetonitrile (16.00 mL). The reaction mixture turned bright yellow in colour and was stirred at room temperature for 1 h. After this time, the reaction mixture cooled in an icebath and DIPEA (7.209 g, 9.716 mL, 55.78 mmol) was added dropwise. The reaction mixture was stirred in the ice bath for 1 h and then additional DIPEA (2.277 g, 3.069 mL, 17.62 mmol) added and the reaction mixture stirred for left for 30 mins and further DIPEA (1.897 g, 2.557 mL, 14.68 mmol) added. The reaction mixture was stirred for Ih and then filtered. The solid was washed with ether, isolated and then triturated with acetonitrile and washed with ether to give the product as a yellow solid (2.776 g, 57 % yield); 1H NMR (400.0 MHz, DMSO) 7.35 (s, 1 H), 7.80 (br s, 2H), 7.98 (m, 1 H), 8.04 (m, 1 H) and 8.45 (s, I H); MS (ES+) 326.04
Step 2: 2-(5-amino-6-(5-(thiophen-2-yl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl)-5-(1,4- diazepane-1-carbonyl)benzonitrile
[00400] tert-Butyl 4-(4-bromo-3-cyano-benzoyl)-l ,4-diazepane-1-carboxylate (126.0 mg, 0.3085 mmol), potassium acetate (90.83 mg, 0.9255 mmol), 4,4,5,5-tetramethyl-2-(4,4,5,5- tetramethyl-1,3,2-dioxaborolan-2-y!)-1,3,2-dioxaborolane (1 17.5 mg, 0.4628 mmol) and 1- cyclopenta-1,4-dienyl-diphenyl-phosphane; dichloromethane; dichloropalladium; iron (25.19 mg, 0.03085 mmol) were heated in dioxane (3 mL) at 80°C for 3 h. Sodium carbonate (462.8 μL of 2 M, 0.9255 mmol) was added to the reaction mixture followed by tert-butyl 4- (4-bromo-3-cyano-benzoyl)-1,4-diazepane-1-carboxylate (126.0 mg, 0.3085 mmol) and palladium; triphenylphosphane (35.65 mg, 0.03085 mmol) and the reaction mixture evacuated and flushed with nitrogen (3 cycles) and then heated at 150°C in the microwave for Ih.
[00401] The reaction mixture was diluted with water (10 mL) and EtOAc (10 ml). The layers were separated and the aqueous layer extracted with EtOAc (3 x 10 mL). The combined organic extracted were dried over MgSθ4, filtered and evaporated to dryness to give a brown/black solid. This solid was re-dissolved in MeOH/DCM (4 mL) and solution filtered through thiol cartridges to leave a brown solid. This was triturated with acetonitrile to give the product as a yellow solid. The yellow solid was redissolved in DCM (3 mL) and TFA (500 μL, 6.490 mmol) added and the resulting mixture stirred at room temperature for 15 mins. This solution was evaporated to dryness and then MeOH/DCM added and the mixture concentrated in vacuo again. The solid was then re-dissolved in acetonitrile/water and passed through a bicarbonate cartridge. The filtrate was then freeze dried to leave the product as a yellow solid (29.8 mg, 20% yield); 1H NMR (400.0 MHz, DMSO) d 1.60 (br s, 1 H), 1.75 (br s, 1 H), 2.70-2.80 (m, 3H), 2.90 (m, 1 H), 3.35 (m, 3H), 3.60-3.70 (m, 2H), 7.30- 7.40 (m, I H), 7.80-7.90 (m, 2H), 7.95-8.15 (m, 5H) and 8.89 (s, 1H) ppm; MS (ES+) 473.26 [00402] The following compounds were all prepared using a method similar to the one described for Compound IA- 152 above.
Compound IA-179 5-(2-methylsulfinylphenyl)-3-[5-(3-methyI-2-thienyl)-1,3,4-oxadiazol-2- yl]pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 2.7 (s, 3H), 3.1 (s, 3H), 7.2-7.25 (m, I H), 7.6-7.8 (m, 3H), 7.9 (s, 1H), 8.05 (d, 1H), 8.25 (d, 1H) and 8.95 (s, I H) ppm; MS (ES+) 398.1
Example 17A: 3-(5-amino-6-(5-phenvl-1,3,4-oxadiazol-2-vl')pvrazin-2-vl)pvridine-4- carbonitrile (Compound IA-153)
SCHEME
Method IV-A Method IV-Q (BoC)2N N-N Steps 1-2
Figure imgf000241_0001
Compound IA-153 Compound IA-153 was prepared using Method IV-A, Steps 1-2, followed by Method IV-N, Step 1 , followed by Method IV-Q, Steps 1-2.
METHOD IV-Q
Step 1: di-ter/-butyl 5-(4-cyanopyridin-3-yl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- yliminodicarbonate
[00403] tert-Butyl N-[5-bromo-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N-tert- butoxycarbonyl-carbamate (150 mg, 0.2894 mmol), 3-(5,5-dimethyl-l ,3,2-dioxaborinan-2- yl)pyridine-2-carbonitrile (75.03 mg, 0.3473 mmol), cesium fluoride (87.92 mg, 0.5788 mmol), copper iodide (5.512 mg, 0.9790 μL, 0.02894 mmol), and palladium; triphenylphosphane (16.72 mg, 0.01447 mmol) were placed in a microwave tube which was evacuated and nitrogen flushed (x5). Dioxane (2.486 mL) was added and the reaction mixture stirred during 5 further vacuum/flush cycles. The resulting solution was heated at 85°C overnight, cooled to room temperature diluted with ethyl acetate. The mixture was washed with aq NaHCC^ solution (1 x 10 mL) and brine (1 x 10 mL), dried over MgSU4 and concentrated under reduced pressure. The material was purified by column chromatography on silica gel eluting 50-60% ethyl acetate/ petroleum ether to give the product as a colourless foam. (136mg, 86.8%); 1H NMR (400.0 MHz, DMSO) 1.34 (s, 18H), 7.19 (s, 2H), 7.49 (m, 3H), 7.75 (m, I H), 8.17 (m, 2H), 8.40 (m, 1H), 8.90 (m, I H) and 9.12 (s, l H) ppm
Step 2: 3-(5-amino-6-(5-phenyl- 1 ,3,4-oxadiazol-2-yl)pyrazin-2-yl)pyridine-4-carbonitrile
[00404] tert-Butyl N-tert-butoxycarbonyl-N-[5-(4-cyano-3-pyridyl)-3-(5-phenyl-l ,3,4- oxadiazol-2-yl)pyrazin-2-yl]carbamate (140 mg, 0.2585 mmol) in dichloromethane (2 mL) was treated with TFA (2 mL, 25.96 mmol) and stirred at room temperature for 1 h. The mixture was concentrated under reduced pressure, re-disolved in MeOH/DCM and concentrated (2 X), dissolved MeOH/DCM and passed through bicarbonate cartridge, concentrated under reduced pressure to give a yellow solid. The solid was triturated with acetonitrile and filtered to give yellow solid (83mg, 94%); 1H NMR (400.0 MHz, DMSO) d 7.69-7.77 (m, 3H), 8.07 (d, I H), 8.24-8.26 (m, 2H), 7.80-8.40 (br s, 2H), 8.90 (d, 1H), 9.09 (s, I H) and 9.47 (s, 1H) ppm; MS (ES+) 342.16 [00405] The following compounds were all prepared using a method similar to the one described for Compound IA-153 above.
Compound IA-74 3-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]pyridine-2- carbonitrile 1H NMR (400.0 MHz, DMSO) d 7.64-7.70 (m, 3H), 7.86-7.89 (m, 1H), 8.18- 8.20 (m, 2H), 8.54-8.57 (m, 1H), 8.79-8.81 (m, 1H) and 8.94 (s, 1H) ppm; MS (ES+) 342.16
Compound IA-132 2-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl]-5- isopropylsulfonyl-benzonitrile 1H NMR (400.0 MHz, CDCl3) d 1.35 (d, 6H), 7.5-7.6 (m, 4H), 8.0-8.05 (m, 1H), 8.1 -8.15 (m, I H), 8.2-8.25 (m, 2H), 8.3-8.32 (m, I H) and 8.7 (s, 1H) ppm; MS (ES+) 447.2
Example 18A: 4-(5-amino-6-(-5-(3-nitrophenvπ-1.3.4-oxadiazol-2-vπpvrazin-2-vπ-N.N- dimethylbenzamide (Compound IA-286)
SCHEME
Figure imgf000243_0001
Compound IA-286
[00406] Compound IA-286 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1.
METHOD IV-R
Step 1: 4-(5-amino-6-(5-(3-nitrophenyl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl)-N,N- dimethy lbenzamide
[00407] Dibromo(triphenyl)phosphorane (707.8 mg, 1.68 mmol) was added to a solution of 3-amino-6-[4-(dimethylcarbamoyl)phenyl]pyrazine-2-carboxylic acid (100 mg, 0.35 mmol) and 4-(5-amino-6-(hydrazinecarbonyl)pyrazin-2-yl)-N,N-dimethylbenzamide (63.28 mg, 0.35 mmol) in acetonitrile (5 mL) at room temperature. A bright yellow solution was observed. The reaction mixture was stirred at room temperarture for 30 min. DIPEA (304 μL, 1.75 mmol) was added dropwise and the reaction mixture was stirred at room temperature for 30 min. The reaction mixture was filtered and the solid triturated with acetonitrile to give the product as a yellow solid (78.5 mg, 51.3 % yield); 1H ΝMR (400.0 MHz, DMSO) d 2.98 (m, 6H), 7.56 (m, 2H), 7.85 (br s, 1H), 7.99 (t, I H), 8.17 (m, 2H), 8.52 (m, I H), 8.56 (m, I H), 8.82 (m, I H) and 9.02 (s, 1H) ppm; MS (ES+) 432.2 [00408] The following compounds were all prepared using a method similar to the one described for Compound IA-286 above.
Compound IA-85 4-[5-amino-6-[5-(3-hydroxyphenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- Ν,Ν-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 3.02 (m, 6H), 7.1 1 (m, 1H), 7.52 (m, 1H), 7.59 (m, 3H), 7.64 (m, 1H), 7.84 (br s, 2H), 8.22 (m, 2H), 9.04 (s, 1H) and 10.13 (1H, s) ppm; MS (ES+) 402.23
Compound IA-180 4-[5-amino-6-(5-thiazol-4-yl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N,N- dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.78 (m, 6H), 7.35 (m, 2H), 7.60 (br s, I H), 7.94 (m, 2H), 8.62 (m, 1H), 8.79 (s, I H) and 9.22 (m, 1H) ppm; MS (ES+) 394.15
Compound IA-187 3-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-y I]- 1 ,3,4- oxadiazol-2-yl]benzonitrile I H NMR (400.0 MHz, DMSO) d 1.20 (d, 6H), 3.47 (q, I H), 7.92 (br s, 2H), 7.90 (t, I H), 7.99 (m, 2H), 8.19 (dt, 1H), 8.41-8.43 (m, 2H), 8.48 - 8.51 (m, I H), 8.58 (t, 1H) and 9.09 (s, 1H) ppm; MS (ES+) 447.2
Compound IA-189 4-[5-amino-6-[5-(3-cyano-2-thienyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.97 (m, 6H), 7.50 (m, 2H), 7.80 (m, I H), 8.21 (m, 3H) and 9.05 (s, 1H) ppm; MS (ES+) 418.15
Compound IA-215 4-[5-amino-6-[5-(2-hydroxyphenyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.97 (s, 3H), 3.01 (s, 3H), 7.09- 7.14 (m, 2H), 7.53-7.55 (m, 3H), 7.80 (br s, 2H), 7.93 (dd, I H), 8.16 (d, 2H), 8.99 (s, I H) and 10.45 (s, I H) ppm; MS (ES+) 403.23 Compound IA-246 4-[5-amino-6-[5-(4-methyl-2-thienyl)-1,3,4-oxadiazol-2-yl]pyrazin-2- yl]-N,N-dimethyl-benzamide I H NMR (400.0 MHz, DMSO) d 2.34 (s, 3H), 2.98 (m, 6H), 7.54 (m, 2H), 7.62 (s, 1H), 7.75 (br s, 2H), 7.85 (s, I H), 8.15 (m, 2H) and 8.98 (s, 1H) ppm; MS (ES+) 407.18
Compound IA-273 4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-l ,3,4- oxadiazol-2-yl]benzonitrile I H NMR (400.0 MHz, DMSO) d 1.20 (d, 6H), 3.48 (m, 1H), 7.99 (d, 2H), 7.80-8.30 (br s, 2H), 8.15 (d, 2H), 8.34 (d, 2H), 8.40 (d, 2H) and 9.09 (s, 1H) ppm; MS (ES+) 447.19
Example 19A: 5-(2-(isopropylsulfinvl')phenvlV3-(5-phenvl-l ,3,4-oxadiazol-2-yl)pyrazin-2- amine (Compound IA-136)
SCHEME
Figure imgf000245_0001
Compound IA-136
Compound IA-136 was prepared using Method IV-A, Steps 1-3, followed by Method IV-S, Step 1.
METHOD IV-S
Step 1: 5-(2-(isopropylsulfinyl)phenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-amine
[00409] 5-(2-isopropylsulfanylphenyl)-3-(5-phenyl-l,3,4-oxadiazol-2-yl)pyrazin-2-amine (50 mg, 0.1284 mmol) was dissolved in dichloromethane (10 mL) and cooled to 0°C in an ice-bath. 3-Chlorobenzenecarboperoxoic acid (26.59 mg, 0.1541 mmol) was added in one portion with rapid stirring. The mixture was stirred at 0°C for 15 min, washed with saturated aqueous NaHCO3 solution (1 x 5 mL) and brine (1 x 5 mL), dried over MgSθ4 and concentrated in vacuo to leave a solid which was purified by column chromatography on silica eluting with ether/ petroleum ether to yield the product as a yellow solid (16.1 mg, 31 % yield); 1H NMR (400.0 MHz, CDCl3) d 1.2 (d, 3H), 1.35 (d, 3H), 3.5-3.6 (m, 1H), 7.5- 7.75 (m, 2H), 7.5-7.65 (m, 7H), 8.25 (d, 2H) and 8.55 (s, 1H) ppm; MS (ES+) 406.1 [00410] The following compounds were all prepared using a method similar to the one described for Compound IA- 136 above.
Compound IA-256 5-(2-ethylsulfinylphenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- amine 1H NMR (400.0 MHz, CDCl3) d 1.25-1.4 (m, 3H), 3.3-3.6 (m, 2H), 7.6-7.8 (m, 6H), 8.2 (d, 2H), 8.4 (d, 1H) and 8.6 (s, 1H) ppm; MS (ES+) 392.2
Example 2OA: 5-(2-isopropylsulfonvIphenvl)-3-f5-phenvl-l .3.4-oxadiazol-2-vl)pyrazin-2- amine (Compound IA-121)
SCHEME
Method IV-A "•*? '*T
Figure imgf000246_0001
Compound IA-121
[00411] Compound IA-121 was prepared using Method IV-A, Steps 1-3, followed by Method IV-T, Step 1.
METHOD IV-T
Step 1: 5-(2-isopropylsulfonylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-amine [00412] 5-(2-isopropylsulfanylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-amine (40 mg, 0.1027 mmol) was dissolved in dichloromethane (10 mL) followed by the portionwise addition of 3-chlorobenzenecarboperoxoic acid (70.89 mg, 0.4108 mmol) over lOmin. The resulting mixture was stirred at room temperature for 2 h, and poured onto a 50/50 mixture of saturated aqueous sodium thiosulfate solution and saturated aqueous sodium bicarbonate solution (20 mL). The layers were separated and the organic layer was washed with dilute NaHCO3 solution (1 x 10 mL) and brine (1 x 10 mL) and then concentrated in vacuo to leave a solid. The solid was purified by column chromatography on silica eluting with 50% ether/petroleum ether to afford the product as a yellow solid. (20.25 mg, 36% yield); 1H NMR (400.0 MHz, CDCl3) d 1.4 (d, 6H), 4.48-4.52 (m, 1H), 7.0 (br s, 2H), 7.5-7.7 (m, 5H), 7.7-7.75 (m, 1H), 8.2 (d, 1H), 8.3 (d, 1H) and 8.55 (s, I H) ppm; MS (ES+) 422.2
[00413] The following compounds were all prepared using a method similar to the one described for Compound IA-121 above.
Compound IA-164 tert-butyl N-[2-[2-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- yl]phenyl]sulfinylethyl]carbamate 1H NMR (400.0 MHz, CDCl3) d 1.55 (s, 9H), 3.45-3.5 (m, I H), 3.6-3.7 (m, 3H), 5.5 (br s, 1H), 7.6-7.8 (m, 6H), 8.2-8.25 (m, 2H), 8.3 (d, 1H) and 8.6 (s, 1H) ppm; MS (ES+) 507.2
Compound IA-203 5-(2-ethylsulfonylphenyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- amine 1 H NMR (400.0 MHz, CDCl3) d 1.2 (d, 3H), 1.35 (d, 3H), 3.5-3.6 (m, 1 H), 7.5-7.75 (m, 2H), 7.5-7.65 (m, 7H), 8.25 (d, 2H) and 8.55 (s, 1H) ppm; MS (ES+) 408.2
Compound IA-280 5-(4-tert-butylsulfonylphenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin- 2-amine 1H NMR (400.0 MHz, DMSO) d 1.32 (s, 9H), 7.7-7.8 (m, 3H), 7.9-8.0 (m, 3H), 8.20-8.25 (m, 2H), 8.45 (d, 2H) and 9.1 (s, 1H) ppm; MS (ES+) 436.2
Example 21A: 4-(5-amino-6-(5-(2-ethoxyphenyl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl)-N,N- dimethylbenzamide (Compound IA-277)
SCHEME
M th d IV C IV R M th d IV U
Figure imgf000247_0001
Compound IA-277 Compound IA-277 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1, followed by Method IV-U, Step 1.
METHOD IV-U: Step 1: 4-(5-amino-6-(5-(2-ethoxyphenyl)-l ,3,4-oxadiazol-2-yl)pyrazin- 2-yl)-N,N-dimethylbenzamide
[00414] Potassium carbonate (25.46 mg, 0.1842 mmol) was added to a mixture of 4-[5- amino-6-[5-(2-hydroxyphenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-Ν,Ν-dimethyl-benzamide (50 mg, 0.1228 mmol) in DMF (1 mL) at room temperature. Colour change observed from yellow to orange. The resulting suspension was heated at 60-65 °C and bromoethane (14.72 mg, 10.01 μL, 0.1351 mmol) was added slowly. After the addition is complete the reaction heated at 60-65 °C for 30 min. The reaction mixture was cooled to room temperature. Water (2mL) was added slowly at and the mixture stirred at room temperature for 20 min. A precipitate formed and was filtered and washed with water. The solid was re-dissolved in DCM and dried over MgSC^, filtered and evaporated to dryness. The solid was triturated with acetonitrile to give the product as a yellow solid (38.3 mg, 73% yield); I H NMR (400.0 MHz, DMSO) d 1.49 (t, 3H), 2.97-3.02 (m, 6H), 4.24-4.29 (m, 2H), 7.17-7.18 (m, 1H), 7.20 (d, 1H), 7.32-7.35 (m, 2H), 7.53 (d, 1H), 7.65 (br s, 2H), 8.05 (d, 1H), 8.17 (d, 2H) and 8.99 (s, 1H) ppm; MS (ES+) 431.24
[00415] The following compounds were all prepared using a method similar to the one described for Compound IA-277 above.
Compound IA-108 4-[5-amino-6-[5-(2-isopropoxyphenyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2- yl]-N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 1.40 (d, 6H), 2.99 (s, 3H), 3.01 (s, 3H), 4.90 (q, I H), 7.20 (t, I H), 7.39 (d, 1H), 7.51-7.53 (m, 2H), 7.55-7.65 (m, I H), 7.80 (br s, 2H), 8.05-8.10 (m, 1H), 8.17-8.20 (m, 2H) and 8.99 (s, 1H) ppm; MS (ES+) 445.27
Compound IA-175 tert-butyl N-[2-[2-[5-[3-amino-6-[4-(dimethylcarbamoyl) phenyl]pyrazin-2-yl]-1,3,4-oxadiazol-2-yl]phenoxy]ethyl]carbamate 1H NMR (400.0 MHz, DMSO) d 1.29 (s, 9H), 2.96 (s, 3H), 3.00 (s, 3H), 3.50 (d, 2H), 4.18 (s, 2H), 6.96-6.99 (m, I H), 7.19-7.25 (m, 1H), 7.35 (d, 1H), 7.53 (d, 2H), 7.66-7.67 (m, I H), 7.80 (br s, 2H), 8.06- 8.08 (m, 1 H), 8.16 (d, 2H) and 8.99 (s, 1 H) ppm; MS (ES+) 546.27 Compound IA-196 4-[5-amino-6-[5-[2-(2-hydroxyethoxy)phenyl]-l ,3,4-oxadiazol-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.98 (s, 3H), 3.00 (s, 3H), 3.86-3.89 (m, 2H), 4.23 (s, 2H), 4.85 (t, 1H), 7.19-7.22 (m, 1H), 7.35-7.37 (m, 1 H), 7.54 (d, 2H), 7.63-7.67 (m, 1 H), 7.79 (br s, 2H), 8.02-8.04 (m, 1 H), 8.18 (s, 2H) and 8.99 (s, 1H) ppm; MS (ES+) 447.24
Compound IA-284 4-[5-amino-6-[5-[2-(3-hydroxypropoxy)phenyl]-1,3,4-oxadiazol-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.00 (s, 2H), 2.97 (s, 3H), 3.01 (s, 3H), 3.65 (d, 2H), 4.26 (s, 2H), 4.55 (s, 1H), 7.19 (s, I H), 7.35 (s, I H), 7.54 (d, 2H), 7.70 (br s, 2H), 8.03 (d, 1H), 8.16 (d, 2H) and 8.98 (s, 1H) ppm; MS (ES+) 461.26
Example 22A: 4-(5-amino-6-(5-(2-aminophenvlV1.3.4-oxadiazol-2-vl)pvrazin-2-vl')-N,N- dimethylbenzamide (Compound IA-99)
SCHEME
Method IV-C IV-V StePS 1 2
Figure imgf000249_0001
[00416] Compound IA-99 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1 , followed by Method IV-V, Step 1.
METHOD IV-V
Step 1: 4-(5-amino-6-(5-(2-aminophenyl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl)-N,N- dimethylbenzamide
[00417] SnCl2.2H2O (151.6 mg, 0.6720 mmol) was added to a solution of4-[5-amino-6- [5-(2-nitrophenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-Ν,Ν-dimethyl-benzamide (58 mg, 0.1344 mmol) in EtOAc (3 mL) and dichloromethane (1 mL) at room temperature and the resulting solution stirred overnight at room temperature. The reaction mixture was poured carefully onto saturated aqueous sodium hydrogen carbonate solution (5 mL) and the layers separated. The aqueous layer extracted further with dichloromethane (2 x 15 mL) and the combined organics dried over MgSO4 and concentrated in vacuo to leave a yellow solid. This was purified using by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. The product fractions were freeze dried to give the product as a yellow solid (17.2 mg, 34 % yield); 1H NMR (400.0 MHz, DMSO) d 2.98 (m, 6H), 6.78 (t, 1H), 6.88 (br s, 1H), 7.34 (m, 1H), 7.55 (m, 2H), 7.79 (br s, 2H), 7.85 (m, 1H), 8.18 (m, 2H) and 8.99 (s, 1H) ppm; MS (ES+) 402.26
The following compounds were all prepared using a method similar to the one described for Compound IA-99 above.
Compound IA-142 4-[5-amino-6-[5-(3-aminophenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 2.98 (m, 6H), 6.88 (m, 1H), 7.31 (m, 2H), 7.41 (m, 1H), 7.56 (m, 2H), 7.78 (br s, 2H), 8.15 (m, 2H) and 8.98 (s, 1H) ppm; MS (ES+) 402.19
Example 23A: 5-(6-(4-methylpiperazin-1-yl)pvridin-3-vπ-3-(5-phenvl-1.3.4-oxadiazol-2- yl)pγrazin-2-amine (Compound IA-200)
SCHEME
Figure imgf000251_0001
Compound IA-200 [00418] Compound IA-200 was prepared using Method IV-A, Steps 1-2, followed by
Method IV-N, Step 1, followed by Method IV-W, Steps 1-2.
METHOD IV-W
Step 1: Di-ter/-butyl(5-(6-(4-methylpiperazin-1-yl)pyridin-3-yl)-3-(5-phenyl-1,3,4- oxadiazol-2-yl)pyrazin-2-yl)iminodicarbonate
[00419] tert-Buty 1 N-[5-bromo-3-(5-pheny 1- 1 ,3,4-oxadiazol-2-yl)pyrazin-2-y l]-N-tert- butoxycarbonyl-carbamate (100 mg, 0.19 mmol) was dissolved in DMF (1 mL) and 1- methyl-4-(5-(4,4,5,5-tetramethyl-l ,3,2-dioxaborolan-2-yl)pyridin-2-yl)piperazine (70.19 mg, 0.23 mmol) and Pd(PPh3)2Cl2 (13.54 mg, 0.019 mmol) were added. K2CO3 (289 μL, 0.58 mmol, 2M aqueous solution) was added and the reaction mixture heated at 100 °C for 12 hours. The reaction mixture was cooled to room temperature and taken onto the next step without further purification. Step 2: 5-(6-(4-methylpiperazin-1-yl)pyridin-3-yl)-3-(5-phenyl-l ,3,4-oxadiazol-2- yl)pyrazin-2-amine
[00420] Di-rer/-butyl(5-(6-(4-methylpiperazin-1-yl)pyridin-3-yl)-3-(5-phenyl-1,3,4- ' oxadiazol-2-yl)pyrazin-2-yl)iminodicarbonate(l 18.6 mg, 0.19 mmol) as a solution in DMF (1 mL) was diluted with dichloromethane (5 mL) and was treated with TFA (1 mL, 12.98 mmol). The resulting solution was stirred at room temperature for 18 h and then concentrated in vacuo. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The fractions were collected and freeze-dried to give the product as a yellow solid (27.3 mg, 27 % yield); 1H NMR (400.0 MHz, DMSO) d 2.87 (d, 3H), 3.08-3.21 (m, 4H), 3.40-3.53 (m, 2H), 4.52 (d, 2H), 7.12 (d, 1H), 7.66-7.70 (m, 5H), 8.16-8.18 (m, 2H), 8.33 (dd, 1H), 8.92 (s, 1H), 8.93 (d, 1H) and 9.75 (br s, 1 H) ppm; MS (ES+) 415.2
[00421] The following compounds were all prepared using a method similar to the one described for Compound IA-200 above.
Compound IA-72 5-[6-(2-morpholinoethylamino)-3-pyridyl]-3-(5-phenyl-1,3,4-oxadiazol- 2-yl)pyrazin-2-amine 1H NMR (400.0 MHz, CDCl3) d 3.17 (br s, 4H), 3.29 (t, 2H), 3.92- 3.94 (m, 6H), 7.03 (d, 1H), 7.51-7.58 (m, 3H), 8.18-8.21 (m, 2H), 8.38 (d, 1H), 8.49 (s, 1H) and 8.52 (s, 1H) ppm; MS (ES+) 445.2
Compound IA-86 5-(3-methoxy-4-pyridyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- amine 1H NMR (400.0 MHz, DMSO) d 4.09 (s, 3H), 7.66-7.72 (m, 3H), 7.96 (br s, 2H), 8.12 (br d, 1H), 8.17 (dd, 2H), 8.47 (d, 1H), 8.64 (s, 1H) and 9.03 (s, 1H) ppm; MS (ES+) 347.1
Compound IA-117 5-(6-methoxy-3-pyridyl)-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2- amine 1H NMR (400.0 MHz, DMSO) d 3.93 (s, 3H), 6.99 (d, 1H), 7.67-7.73 (m, 5H), 8.17- 8.20 (m, 2H), 8.42 (dd, 1H), 8.93 (d, 1H) and 8.95 (s, 1H) ppm; MS (ES+) 347.1
Compound IA-165 5-[2-(2-dimethylaminoethyloxy)-4-pyridyl]-3-(5-phenyl-1,3,4- oxadiazol-2-yl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 2.23 (s, 6H), 2.65 (t, 2H), 4.41 (t, 2H), 7.49 (s, 1 H), 7.69-7.73 (m, 4H), 7.95 (br s, 2H), 8.16-8.19 (m, 2H), 8.28 (d, 1 H) and 9.07 (s, 1H) ppm; MS (ES+) 404.1
Compound IA-186 3-(5-phenyl-1,3,4-oxadiazol-2-yl)-5-(6-piperazin-1-yl-3-pyridyl)pyrazin- 2-amine 1H NMR (400.0 MHz, DMSO) d 3.00 (br d, 4H), 3.58 (br t, 4H), 6.86 (d, I H), 7.42- 7.48 (m, 5H), 7.94 (dd, 2H), 8.09 (dd, 1H), 8.53 (br s, 2H), 8.68 (s, 1H) and 8.69 (d, 1H) ppm
Compound IA-265 N'-[4-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-2- pyridyl]-N,N-dimethyl-ethane-l ,2-diamine 1H NMR (400.0 MHz, DMSO) d 2.20 (s, 6H), 2.45 (t, 2H), 3.41 (q, 2H), 6.54 (t, 1H), 7.18 (dd, 1H), 7.21 (s, 1H), 7.67-7.74 (m, 3H), 7.86 (br s, 2H), 8.08 (d, I H), 8.18 (dd, 2H) and 8.89 (s, 1H) ppm; MS (ES+) 403.2
Compound IA-279 5-[6-[3-(dimethylamino)propoxy]-3-pyridyl]-3-(5-phenyl- 1,3,4- oxadiazol-2-yl)pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 1.89 (quin, 2H), 2.16 (s, 6H), 2.36 (t, 2H), 4.35 (t, 2H), 6.96 (d, I H), 7.68-7.71 (m, 5H), 8.17-8.19 (m, 2H), 8.40 (dd, 1H), 8.91 (d, I H) and 8.94 (s, I H) ppm; MS (ES+) 418.2
Compound IA-283 5-(6-morpholino-3-pyridyl)-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2- amine 1H NMR (400.0 MHz, DMSO) d 3.33-3.35 (m, 4H), 3.52-3.54 (m, 4H), 6.79 (d, 1H), 7.42-7.51 (m, 5H), 7.95-7.97 (m, 2H), 8.07 (dd, 1H) and 8.69 (s, 2H) ppm; MS (ES+) 402.1
Example 24A: 5-(2-(2-aminoethylthio)phenyO-3-(5-phenvl-1.3.4-oxadiazol-2-yl)pyrazin-2- amine (Compound IA-224)
SCHEME
Method IV-Q
Figure imgf000253_0001
Compound IA-224 [00422] Compound IA-224 was prepared using Method IV-A, Steps 1-3, followed by Method IV-Q, Step 2.
Compound IA-224 5-(2-(2-aminoethylthio)phenyl)-3-(5-phenyl-1,3,4-oxadiazol-2- yl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 2.9-3.0 (m, 2H), 3.2 (t, 2H), 7.8 (t, I H), 7.95 (t, 1H), 7.6-7.75 (m, 5H), 7.75-7.85 (br s, 3H), 8.15 (d, 2H) and 8.55 (s, I H) ppm; MS (ES+) 391.2
Example 25A: 5-(2-(2-aminoethylsulfinvπphenvD-3-(5-phenvl-1.3.4-oxadiazol-2- yl)pyrazin-2-amine (Compound IA-261)
Method IV-A Method IV-S
Figure imgf000254_0001
Compound IA-261
Compound IA-261 was prepared using Method IV-A, Steps 1-3, followed by Method IV-S, Step 1 , followed by Method IV-Q, Step 2.
Compound IA-261 5-(2-(2-aminoethylsulfinyl)phenyl)-3-(5-phenyl-l ,3,4-oxadiazol-2- yl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 2.9-3.0 (m, 1H), 3.1-3.2 (m, I H), 3.3- 3.4 (m, 1H), 3.55-3.62 (m, I H), 7.6-7.75 (m, 8H), 7.8-7.9 (m, 2H), 8.2-8.3 (m, 4H) and 8.55 (s, 1H) ppm; MS (ES+) 407.2
Example 26A: 4-r5-amino-6-r5-(6-amino-2-pyridv0-1,3,4-oxadiazol-2-vl]pvrazin-2-vll- N,N-dimethyl-benzamide (Compound IA-231)
SCHEME
M Meettnhooαd i IVv--iC X ' A " NH2 Me .th. od, . I.V. X„
Figure imgf000255_0001
Compound IA-231
[00423] Compound IA-231 was prepared using Method IV-C, Steps 1-2, followed by
Method IV-X, Steps 1-2.
METHOD IV-X
Step 1: 4-(5-amino-6-(2-(6-aminopyridine-2-carbonyl)hydrazinecarbonyl) pyrazin-2-yl)-
N,N-dimethylbenzamide
[00424] A solution of 4-[5-amino-6-(hydrazinecarbonyl)pyrazin-2-yl]-Ν,Ν-dimethyl- benzamide (100 mg, 0.3163 mmol) and 6-aminopyridine-2-carboxylic acid (43.69 mg, 0.3163 mmol) in DMF (1.000 mL) weas treated with triethylamine (32.01 mg, 44.09 μL, 0.3163 mmol) then warmed slightly. The mixture was cooled to room temperature and then treated with (benzotriazol-1 -yloxy-dimethylamino-methylene)-dimethyl-arnmonium tetrafluoroborate (121.9 mg, 0.3796 mmol) and stirred at room temperature overnight. The mixture was poured dropwise onto water (5ml) with rapid stirring, stirred at room temperature for 1 h then filtered to give a wet paste which was dried under high vacuum at 100 °C overnight. Used directly in the next step without purification.
Step 2: 4-[5-amino-6-[5-(6-amino-2-pyridyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N- dimethyl-benzamide
[00425] To a suspension of 4-[5-amino-6-[[(6-aminopyridine-2-carbonyl)amino] carbamoyl]pyrazin-2-yl]-N,N-dimethyl-benzamide (80 mg, 0.1903 mmol) in anhydrous acetonitrile (1.600 mL) cooled in an ice bath, was added DIPEA (73.78 mg, 99.43 μL, 0.5709 mmol) followed by dibromo(triphenyl)phosphorane (104.4 mg, 0.2474 mmol) portionwise. The resutling mixture was stirred at room temperature for 30 min. The precipitate was isolated by filtration, washed with small amount of acetonitrile giving a pale yellow solid. The solid was triturated in hot acetonitrile, cooled, filtered and washed acetonitrile then ether to give the pure product (36.1 mg, 54 % yield); 1H NMR (400 MHz, DMSO) d 2.98-3.02 (m, 6H), 6.53 (br s, 2H), 6.69 (m, 1.5H), 7.16 (m, 0.5H), 7.16 (m, I H), 7.53-7.66 (m, 3H), 8.1 1 (m, 2H) and 8.94 (s, I H) ppm; MS (ES+) 403.29 [00426] The following compounds were all prepared using a method similar to the one described for Compound IA-231 above.
Compound IA-73 tert-butyl N-[[3-[5-[3-amino-6-[4-(dimethylcarbamoyl)phenyi]pyrazin-2- yl]-1,3,4-oxadiazol-2-yl]phenyl]methyl]carbamate I H NMR (400.0 MHz, DMSO) d 1.41 (s, 9H), 2.98 (m, 6H), 4.27 (m, 2H), 7.54-7.60 (m, 4H), 7.65 (m, 1H), 7.79 (br s, 2H), 8.03-8.05 (m, 2H), 8.16 (m, 2H)'and 8.99 (s, I H) ppm; MS (ES+) 516.3
Compound IA-95 tert-butyl 4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- 1,3,4-oxadiazol-2-yl]-3,6-dihydro-2H-pyridine-1-carboxylate 1H NMR (400 MHz, DMSO) d 1.19 (d, 6H), 1.45 (s, 9H), 2.64 (s, 2H), 3.39-3.53 (m, 1H), 3.60 (s, 2H), 4.18 (s, 2H), 7.01 (s, 1H), 7.85 (s, 2H), 7.95 (d, 2H), 8.36 (d, 2H) and 9.05 (s, 1H) ppm; MS (ES+) 527.3
Compound IA-143 5-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-l ,3,4- oxadiazol-2-yl]thiophene-3-carbonitrile 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 3.47 (t, 1H), 7.97 (d, 2H), 8.40 (dd, 2H), 8.46 (d, 1H), 8.97 (d, 1H) and 9.08 (s, 1H) ppm; MS (ES+) 453.0
Compound IA-154 tert-butyl N-[l-[3-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2- yl]-1,3,4-oxadiazol-2-yl]phenyl]ethyl]carbamate 1H NMR (400 MHz, DMSO) d 1.19 (m, 6H), 1.38 (m, 12H), 3.48 (m, 1H), 4.77 (m, 1H), 7.64 (m, 3H), 7.97 (m, 2H), 8.03 (m, 1H), 8.10 (s, 1H), 8.39 (m, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 565.35
Compound IA-162 tert-butyl N-[[4-[5-[3-amino-6-[4-(dimethylcarbamoyl)phenyl]pyrazin- 2-yl]-l ,3,4-oxadiazol-2-yl]phenyl]methyl]carbamate 1H NMR (400.0 MHz, DMSO) d 1.42 (s, 9H), 2.98-3.02 (m, 6H), 4.25 (d, 2H), 7.51-7.56 (m, 5H), 7.79 (br s, 2H), 8.12-8.19 (m, 4H) and 8.98 (s, 1H) ppm; MS (ES+) 516.24
Compound IA-223 4-[5-amino-6-[5-(2-amino-4-pyridyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2- yl]-N,N-dimethyl-benzamide 1H NMR (400 MHz, DMSO) d 2.98-3.02 (m, 6H), 6.49 (br s, 2H), 7.13-7.17 (m, 2H), 7.54-7.56 (m, 2H), 7.80 (br s, 2H), 8.14-8.19 (m, 3H) and 9.00 (1H, s) ppm; MS (ES+) 403.21
Compound IA-251 tert-butyl N-[2-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- 1,3,4-oxadiazol-2-yl]ethyl]carbamate 1H NMR (400 MHz, DMSO) d 1.19 (d, 6H), 1.32 (s, 9H), 2.08 (s, 2H), 3.13 (s, 2H), 3.31 (d, 2H), 3.40 (d, 1H), 7.05-7.08 (m, 1H), 7.96 (d, 2H), 8.32 (d, 2H) and 9.02 (s, 1H) ppm; MS (ES+) 489.27
Example 27A: 4-(5-amino-6-(5-(2-ethvlphenvO-1.3.4-oxadiazol-2-vl)pvrazin-2-vO-N,N- dimethylbenzamide (Compound IA-155)
SCHEME
IV-Y
Figure imgf000257_0001
Compound IA-155
[00427] Compound IA-155 was prepared using Method IV-B, Steps 1-2, followed by Method IV-L, Step 1, followed by Method IV-O, Step 1, followed by Method IV-Y, Step 1.
METHOD IV-Y
Step 1: 4-(5-amino-6-(5-(2-ethylphenyl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl)-N,N- dimethylbenzamide
[00428] A suspension of 4-[5-amino-6-[5-(2-vinylphenyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2- yl]-Ν,Ν-dimethyl-benzamide (45 mg, 0.1091 mmol) in a mixture of ethanol (5 mL) and acetic acid (0.5 mL) in the presence of Pd on C, wet, Degussa (1 16.1 mg, 0.1091 mmol) was hydrogenated at 60psi overnight using the parr apparatus. The reaction mixture was filtered through a Celite pad and washed with more ethanol (5 mL) and ethyl acetate (5mL). The filtrate was dried over MgSO 4 and filtered and concentrated in vacuo. Purified by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were combined and freeze dried to give the product as a yellow solid (14.2 mg, 38 % yield); 1H NMR (400 MHz, DMSO) d 1.30 (t, 3H), 2.98 (m, 6H), 3.13 (q, 2H), 7.48-7.55 (m, 4H), 7.62 (m, I H), 7.78 (br s, 2H), 8.05 (m, 1H), 8.14 (m, 2H) and 8.99 (1H, s) ppm; MS (ES+) 415.27
Example 28A: 4-15-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yll-N,N-dimethyl-3- prop-1-ynyl-benzamide (Compound IA-268)
Method IV-A Method IV-N (Boc>2N N-N Method N-N
Steps 1-2 Step i
Figure imgf000258_0001
Compound IA-268
[00429] Compound IA-268 was prepared using Method IV-A, Steps 1-2, followed by Method IV-N, Step 1 , followed by Method IV-Z, Steps 1-2. METHOD IV-Z
Step 1: di-tert-butyl(5-(4-(dimethylcarbamoyl)-2-(prop-1-ynyl)phenyl)-3-(5-phenyl-l ,3,4- oxadiazol-2-yl)pyrazin-2-yl)iminodicarbonate
[00430] 4-(dimethylcarbamoyl)-2-(prop-1-ynyl)phenylboronic acid (52 mg, 0.225 mtnol) was treated with tert-butyl N-[5-bromo-3-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N- tert-butoxycarbonyl-carbamate (77.91 mg, 0.1503 mmol), sodium carbonate (75.15 μL of 2 M, 0.1503 mmol) and dichloropalladium; triphenylphosphane (10.55 mg, 0.01503 mmol) and DMF (1.122 mL), and the resulting mixture heated at 95 °C in the microwave for 1 h. The reaction mixture was diluted with EtOAc and washed NaHCθ3/NaCl aqueous solution (3 x 5 mL), dried over MgSO4 and concentrated in vacuo. Purified by column chromatography on silica gel eluting with 30- 100%EtO Ac/petroleum ether. Product fractions were combined and concentrated in vacuo to leave a brown oil which was used directly in the next step.
Step 2: 4-[5-amino-6-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N,N-dimethyl-3-prop-1- ynyl-benzamide
[00431] di-tø/^butyl(5-(4-(dimethylcarbamoyl)-2-(prop-1-ynyl)phenyl)-3-(5-phenyl- 1,3,4-oxadiazol-2-yl)pyrazin-2-y!)iminodicarbonate (10 mg, 0.01601 mmol) in CH2CI2 (200 μL) was treated with TFA (200 μL, 2.596 mmol) and stirred at room temperature for 1 h. The reaction mixture was concentrated under reduced pressure then passed through carbonate cartridge with eluting with DCM/MeOH. The product was purified by column chromatography on silica gel eluting with 20-100% EtOAc/CH2Cl2 to yield the product (2.6 mg, 34% yield); 1H NMR (400 MHz, CDCl3) d 1.97 (s, 3H), 2.98-3.07 (m, 6H), 7.40-7.52 (m, 5H), 7.82 (m, I H), 8.19 (m, 2H) and 8.90 (s, 1H) ppm; MS (ES+) 425.21
Example 29A: 4-r5-amino-6-r5-r3-(aminomethv0phenyπ-1.3,4-oxadiazol-2-vllpyrazin-2- yl]-N,N-dimethyl-benzamide (Compound IA-183)
Method IV-B
Figure imgf000260_0001
Compound IA-183
Compound IA-183 was prepared using Method IV-B, Steps 1-4, followed by Method IV-AA, Step 1.
METHOD IV-AA
Step 1: 4-[5-amino-6-[5-[3-(aminomethyI)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N- dimethyl-benzamide
[00432] TFA (131.1 mg, 88.58 μL, 1.150 mmol) was added to a solution of tert-butyl N- [[3-[5-[3-amino-6-[4-(dimethylcarbamoyl)phenyl]pyrazin-2-yl]-l ,3,4-oxadiazol-2- yl]phenyl]methyl]carbamate (60 mg, 0.1 150 mmol) in dichloromethane (2 mL) and the resulting solution stirred at room temperature overnight. The reaction mixture was passed through a bicarbonate cartridge, which was flushed further with methanol (5 mL). The filtrate was concentrated in vacuo to leave a yellow/orange solid. The solid was taken up in a mixture of methanol and dichloromethane and passed through an SCX cartridge. The cartridge was washed initially with methanol and then the product eluted with 2M ammonia in methanol solution over 4 fractions. A yellow solid crystallised out of the filtrate which was isolated by filtration to give the product (44 mg, 90% yield) 1 H NMR (400 MHz, DMSO) d 2.98 (m, 6H), 3.87 (s, 2H), 7.55 (m, 2H), 7.59-7.66 (m, 2H), 7.81 (br s, 2H), 8.00 (m, I H), 8.17 (m, 3H) and 8.99 (s, I H) ppm; MS (ES+) 416.26
[00433] The following compounds were all prepared using a method similar to the one described for Compound IA-183 above. Compound IA-234 4-[5-amino-6-[5-[4-(aminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin- 2-yl]-N,N-dimethyl-benzamide I H NMR (400 MHz, DMSO) d 2.99-3.02 (m, 6H), 4.20 (s, 2H), 7.56 (d, 2H), 7.75 (d, 2H),7.80 (br s, 2H), 8.18 (d, 2H), 8.23 (d, 2H), 8.34 (br s, 2H) and 9.00 (s, I H) ppm; MS (ES+) 416.25
Example 3OA: 4-r5-amino-6-f5-r2-(2-aminoethoxv')phenvll-l .3.4-oxadiazol-2-vllpvrazin-2- yl]-N,N-dimethyl-benzamide (Compound IA-213)
SCHEME
Method IV-C Method IV-R Method IV-AB 1 M
Figure imgf000261_0001
Compound IA-213
[00434] Compound IA-213 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1 , followed by Method IV-AB, Step 1.
METHOD IV-AB
Step 1: 4-[5-amino-6-[5-[2-(2-aminoethoxy)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N- dimethyl-benzamide
[00435] A mixture of 4-[5-amino-6-[5-(2-hydroxyphenyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2- yl]-N,N-dimethyl-benzamide (50 mg, 0.1228 mmol) in DMF (1.000 mL) was stirred at room temperature and potassium carbonate (25.46 mg, 0.1842 mmol) added. The resulting suspension was heated at 60-65 °C and tert-butyl N-(2-bromoethyl)carbamate (30.28 mg, 0.1351 mmol) was added slowly. After addition is complete the reaction mixture was heated overnight at 65°C. The reaction mixture was cooled to room temperature and water (2mL) added slowly and the mixture stirred at room temperature for 20 min. A precipitate formed and was isolated by filtration and washed with water (3 x 5 mL). The solid was re-dissolved in CH2CI2 and dried (MgSCM), filtered and evaporated to dryness. The solid was triturated with DCM/Ether, and filtered to leave a yellow solid. The yellow solid was in CH2CI2 (1 mL) and TFA (150 μL, 1.947 mmol) added and the resulting solution stirred at room temperature for 2 h. The reaction mixture was concentrated in vacuo and the residue taken up in a mixture of MeOH/ CH2Ch (4 mL) and passed through a bicarbonate cartridge eluting with MeOH/DCM. The filtrate was evaporated to dryness and then triturated with acetonitrile to give the product as a yellow solid (36.7 mg, 69% yield) 1 H NMR (400 MHz, DMSO) d 2.98 (s, 3H), 3.02 (s, 3H), 3.31 (t, 2H), 4.41 (t, 2H), 7.30 (t, I H), 7.40 (d, 1H), 7.56 (d, 2H), 7.69 - 7.71 (m, 2H), 7.87 (s, 3H), 8.04 (dd, I H), 8.16 (d, 2H) and 9.01 (s, I H) ppm; MS (ES+) 446.28
Example 31A: 4-(5-amino-6-(5-(3-methoxythiophen-2-vl)-1,3,4-oxadiazol-2-yl)pyrazin-2- yl)-N,N-dimethylbenzamide (Compound IA-172)
SCHEME
Figure imgf000262_0001
Compound IA-172
[00436] Compound IA- 172 was prepared using Method IV-C, Steps 1 -2, followed by Method IV-AC, Step 1.
METHOD IV-AC
Step 1: 4-(5-amino-6-(5-(3-methoxythiophen-2-yl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl)-N,N- dimethylbenzamide
[00437] TBTU (160.4 mg, 0.4995 mmol) and Et3N (33.70 mg, 46.42 μL, 0.3330 mmol) were added to a solution of 4-(5-amino-6-(hydrazinecarbonyl)pyrazin-2-yl)-N,N- dimethylbenzamide (100 mg, 0.3330 mmol) and 3-methoxythiophene-2-carboxylic acid (52.67 mg, 0.3330 mmol) in CH2Cl2 (2.000 mL) and the resulting solution stirred at room temperature for 72 h. The reaction mixture was diluted with dichloromethane (5 mL) and water (5 mL) and the layers separated. The aqueous layer was extracted further with dichloromethane (3 x 5 mL), and the combined organic extracts dried over MgSθ4, filtered and concentrated in vacuo to leave 4-[5-amino-6-[[(3-methoxythiophene-2- carbonyl)amino]carbamoyl]pyrazin-2-yl]-N,N-dimethyl-benzamide as a yellow oil. POCb (1.788 g, 1.087 mL, 1 1.66 mmol) was added to 4-[5-amino-6-[[(3-methoxythiophene-2- carbonyl)amino]carbamoyl]pyrazin-2-yl]-N,N-dimethyl-benzamide and the resulting mixture heated at 100 °C for 2h. The reaction mixture was cooled to room temperature and ice/water added carefully with vigourous stirring between additions. The mixture was left to stir at room temperature for 20 min and then diluted with dichloromethane (10 mL) and the layers separated. The aqueous layer was extracted further with dichloromethane (2 x 5 mL) and combined organics dried over MgSO 4 and concentrated in vacuo. Solid obtained is re- dissolved in CH2Cl2 and purified by column chromatography on the ISCO column companion system (12 g column, 0-5% MeOH/ CH2Cl2). Product fractions were combined and concentrated in vacuo. This was purified further by reverse phase preparative HPLC [Waters Sunfire C 18, 1OmM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were combined and freeze dried to give the product as a yellow solid (33.0 mg, 23 % yield) I H NMR (400 MHz, DMSO) d 2.98 (s, 3H), 3.01 (s, 3H), 4.07 (s, 3H), 7.30 (d, 1H), 7.56 (d, 2H), 7.70 (br s, 2H), 7.96 (d, 1H), 8.14 (d, 2H) and 8.97 (s, 1H) ppm; MS (ES+) 423.19
Example 31 A: 2-(5-amino-6-(5-(3-methvlthiophen-2-vl)-1.3.4-oxadiazol-2-yl)pyrazin-2-vO- 5-(1,4-diazepane-1-carbonyl)benzonitrile (Compound IA-181)
SCHEME
Figure imgf000264_0001
Compound IA-181 was prepared using Method IV-AD, Steps 1-5.
METHOD IV-AD
Step 1 : 3-amino-6-bromo-N'-(3-methy lthiophene-2-carbonyl)pyrazine-2-carbohydrazide
[00438] To a suspension of 3-amino-6-bromo-pyrazine-2-carboxylic acid (13.26 g, 60.82 mmol) and 3-methylthiophene-2-carbohydrazide (1O g, 60.82 mmol) in DMF (95.00 mL) cooled in an ice bath was added Et3N (7.385 g, 10..17 mL, 72.98 mmol) followed by TBTU (23.43 g, 72.98 mmol) portionwise after complete addition, the reaction mixture was allowed to warm to room temperature and stirred overnight. The reaction mixture was diluted with EtOAc (50 mL) and water (50 mL). The layers were separated and the organic extracts washed with water (1 x 50 mL) and brine (1 x 50 mL), dried over MgSC>4 and concentrated to leave a brown solid (7.85 g, 36% yield) which was used directly in the next step without further purification.
Step 2: 5-bromo-3-(5-(3-methylthiophen-2-yl)-1,3,4-oxadiazol-2-yl)pyrazin-2-amine
[00439] To a suspension of 3-amino-6-bromo-N'-(3-methylthiophene-2- carbonyl)pyrazine-2-carbohydrazide (7.85 g, 22.04 mmol) in anhydrous acetonitrile (1 17.8 mL) cooled in an ice bath was added DIPEA (8.546 g, 11.52 mL, 66.12 mmol) followed by dibromo(triphenyl)phosphorane (12.09 g, 28.65 mmol) portionwise. The resulting suspension was stirred at room temperature for 30 min and the precipitate isolated by filtration and washed with acetonitrile to give the product as a yellow solid (4.42 g, 52% yield); I H NMR (400 MHz, DMSO) d 2.64 (s, 3H), 7.21 (d, '1H), 7.91 (3H, m) and 8.44 (s, 1H) ppm; MS (ES+) 340.04
Step 3: tert-butyl-5-bromo-3-(5-(3-methylthiophen-2-yl)-l ,3,4-oxadiazol-2-yl)pyrazin-2- yl(teτt-butoxycarbonyl)carbamate
[00440] 5-bromo-3-[5-(3-methyl-2-thienyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-amine (10.68 g, 31.58 mmol) and DMAP (385.8 mg, 3.158 mmol) were suspended in CH2Cl2 (160.2 mL) and THF (160.2 mL) and cooled in an icebath. tert-Butoxycarbonyl tert-butyl carbonate (20.68 g, 94.74 mmol) was added portionwise to the stirred mixture. The reaction mixture was stirred at room temperature for Ih and then diluted with CH2Cl2 (100ml) and saturated aqueous sodium hydrogen carbonate solution (100ml). The layers were separated and the organic layer washed with saturated aqueous sodium hydrogen carbonate solution (2 x 100ml), dried over MgSC^, filtered and concentrated in vacuo. The residue was recrystallised from a mixture of ethyl acetate and petroleum ether to give the product as a brown crystalline material (14.29 g, 84% yield); 1H NMR (400 MHz, DMSO) d 1.41 (s, 9H), 2.72 (s, 3H), 7.10 (m, 1 H), 7.55 (m, 1 H) and 8.74 (s, 1 H) ppm
Step 4: tert-butyl 4-(4-(5-(bis(tert-butoxycarbonyl)amino)-6-(5-(3-methylthiophen-2-yl)- 1,3,4-oxadiazol-2-yl)pyrazin-2-yl)-3-cyanophenylcarbonyl)-1,4-diazepane-1-carboxylate [00441] tert-Butyl N-[5-bromo-3-[5-(3-methyl-2-thienyl)-1,3,4-oxadiazol-2-yl]pyrazin-2- yl]-Ν-tert-butoxycarbonyl-carbamate (13.52 g, 25.12 mmol) and tert-butyl 4-[3-cyano-4- (4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzoyl]-1,4-diazepane-1-carboxylate (11.44 g, 25.12 mmol) were taken up in DMF (160 mL) and Na2CO3 (37.68 mL of 2 M, 75.36 mmol) (4:1 mixture) and reaction mixture degassed with nitrogen and Pd (tBu3P)2 (1.027 g, 2.010 mmol) added in one portion. The resulting mixture was heated at 75°C 2.5 h. The reaction mixture was cooled to room temperature and diluted with EtOAc (50 mL) and water (50 mL). The organic extracts were washed with water (1 x 100 mL) and brine (1 x 100 mL) and then the aqueous layers back extracted with ethyl acetate (3 x 100 mL), dried over MgSC>4, filtered and concentrated in vacuo. The material was passed through a silica pad eluting with 50-100% EtOAc / Petroleum ether. The material was purified further by column chromatography on silica (500 mL) eluting with 30-100% EtOAc / petroleum ether. Product fractions were combined and concentrated in vacuo to leave the product as a yellow solid (11.9 g, 55% yield)
Step 5: 2-[5-amino-6-[5-(3-methyl-2-thienyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]-5-(1,4- diazepane- 1 -carbony l)benzonitrile
[00442] tert-Butyl 4-[4-[5-[bis(tert-butoxycarbonyl)amino]-6-[5-(3-methyl-2-thienyl)- l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]-3-cyano-benzoyl]-1,4-diazepane-1-carboxylate (9.9 g, 1 1.32 mmol) was dissolved in anhydrous CH2CI2 (100 mL) at room temperature and TFA (10 mL, 129.8 mmol) added. Additional TFA (10 mL, 129.8 mmol) was added and the reaction mixture stirred at room temperature for 3.5 h and then concentrated in vacuo. The material was dissolved in a mixture of acetonitrile and methanol (10; 1 mixture) and PS- HCO3 (5 eq) added. The mixture was stirred for Ih at room temperature and then the resin removed by filtration and washed with acetonitrile and methanol. The filtrate was concentrated in vacuo and the residue recrystallised from acetonitrile. The isolated solid was washed with ether and dried to give the product as a yellow solid (2.41 g, 35 % yield); 1 H NMR (400 MHz, DMSO) d 1.76-1.84 (m, 2H), 2.67 (s, 3H), 2.88-2.93 (m, 4H), 3.42-3.44 (m, 2H), 3.67-3.74 (m, 2H), 7.2 (d, 1H), 7.84-7.87 (m, 1H), 7.89-7.99 (m, I H), 8.04-8.09 (m, 2H) and 8.85 (s, 1H) ppm; MS (ES+) 487.26
Example 32A: l-r5-amino-6-(5-phenvl-1.3.4-oxadiazol-2-vl)pyrazin-2-vllpvrrole-2- carbonitrile (Compound IA-264)
SCHEME
Method IV-A Steps 1-2
Figure imgf000267_0001
Compound IA-264
Compound IA-264 was prepared using Method IV-A, Steps 1-2, followed by Method IV-N, Step 1, followed by Method IV-AE, Step 1.
METHOD IV-AE
Step 1: l-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]pyrrole-2-carbonitrile [00443] tert-Butyl N-[5-bromo-3-(5-phenyl-l ,3,4-oxadiazol-2-yl)pyrazin-2-yl]-N-tert- butoxycarbonyl-carbamate (100 mg, 0.1929 mmol) and cesium carbonate (125.7 mg, 0.3858 mmol) were added to DMF (5 mL) followed by the addition of 1H-pyrrole-2-carbonitrile (26.65 mg, 0.2894 mmol). The resulting mixture was heated at 5O°C for Ih. The mixture was cooled to room temperature, filtered and diluted with ethyl acetate (5 mL). The organics were washed with water (1 x 10 mL) and brine (1 x 10 mL) and the organic layer concentrated in vacuo to leave an oil. This was dissolved in CH2CI2 (10 mL) and TFA (659.9 mg, 445.9 μL, 5.787 mmol) was added. The reaction mixture was stirred at room temperature for Ih, and then concentrated in vacuo to an oil. The oil was dissolved in CH2CI2 and the product precipitated by slow addition of petroleum ether (28.3 mg, 45 % yield); 1H NMR (400 MHz, DMSO) d 6.6 (s, 1 H), 7.3 (s, 1H), 7.7-7.85 (m, 3H), 7.9 (br s, 2H), 7.95 (s, 1H), 8.2-8.25 (m, 2H) and 8.8 (s, 1H) ppm; MS (ES+) 330.2
Example 33A: 4-r5-amino-6-(5-phenvl-l .3.4-oxadiazol-2-vl)pyrazin-2-vll-2-(2- dimethylaminoethylamino)pyridine-3-carbonitrile (Compound IA-209)
SCHEME
Method IV-Q
Steps 1-2
Figure imgf000268_0001
Compound IA-209
[00444] Compound IA-209 was prepared using Method IV-A, Steps 1-2, followed by Method IV-N, Step 1 , followed by Method IV-Q, Step 1, followed by Method IV-AF, Step 1.
METHOD IV-AF
Step 1: 4-[5-amino-6-(5-phenyl-1,3,4-oxadiazol-2-yl)pyrazin-2-yl]-2-(2- dimethylaminoethylamino)pyridine-3-carbonitrile
[00445] N,N-dimethylethane-1,2-diamine (22.04 mg, 27.45 μL, 0.2500 mmol) was added to a solution of di-tert-butyl 5-(2-chloro-3-cyanopyridin-4-yl)-3-(5-phenyl-l ,3,4-oxadiazol-2- yl)pyrazin-2-yliminodicarbonate (36 mg, 0.06250 mmol) and Et3N (25.30 mg, 34.85 μL, 0.2500 mmol) in NMP (1 mL) and the reaction was heated at 150 °C for 2 hours under microwave conditions. The material was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. The fractions were collected and freeze-dried to give the title compound as a yellow solid (7.6 mg, 24% yield); 1H NMR (400 MHz, DMSO) d 2.88 (d, 6H), 3.38 (br m, 2H), 3.81-3.83 (m, 2H), 7.34 (d, 1H), 7.63- 7.71 (m, 3H), 8'.22-8.24 (m, 2H), 8.38 (d, 1H), 8.96 (s, 1H) and 9.23 (br s, 1H) ppm; MS (ES+) 428.3
Example 34A: 4-r5-amino-6-r5-r3-(-hvdroxvmethvπphenvll-1.3.4-oxadiazol-2-vllpvrazin-2- yl]-N,N-dimethyl-benzamide (Compound IA-198)
SCHEME
Method IV-C
Figure imgf000269_0001
Compound IA-198
[00446] Compound IA-198 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1, followed by Method IV-AG, Step 1.
METHOD IV-AG: Step 1: 4-[5-amino-6-[5-[3-(hydroxymethyl)phenyl]-1,3,4-oxadiazol-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzamide
[00447] Diisobutylaluminium hydride (810.0 μL of 1 M, 0.8100 mmol) in dichloromethane was added dropwise to a solution of methyl 3-[5-[3-amino-6-[4- (dimethylcarbamoyl)phenyl]pyrazin-2-yl]-1,3,4-oxadiazol-2-yl]benzoate (120 mg, 0.2700 mmol) in CH2CI2 (6 mL) at 0 °C, the solution darkened upon addition. The resulting solution was stirred at 0 °C for 30 min and allowed to warm slowly to room temperature. The reaction mixture was stirred at room temperature for 4 h and then quenched by addition of IM HCl (3 mL). The resulting mixture was filtered through a Celite pad and washed with dichloromethane (2 x 5 mL). The layers were separated and the aqueous layer extracted further with dichloromethane (2 x 10 mL) and combined organic extracts dried over MgSO4 and concentrated in vacuo. The residue was purified using by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were combined and lyopholised to give the product as a yellow solid (20.3 mg, 18% yield); 1H NMR (400 MHz, DMSO) d 2.98 (m, 6H), 4.66 (s, 2H), 7.55 (m, 2H), 7.63 (m, 2H), 7.80 (br s, 2H), 8.04 (m, I H), 8.16 (m, 3H) and 8.99 (s, 1H) ppm; MS (ES+) 417.23
Example 35A: 4-r5-amino-6-r5-r3-(2-hvdroxvethyl)phenvll-l .3.4-oxadiazol-2-vπpvrazin-2- yl]-N,N-dimethyl-benzamide (Compound IA-69)
SCHEME
Figure imgf000270_0001
Compound IA-69
[00448] Compound IA-69 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1, followed by Method IV-AH, Step 1.
METHOD IV-AH
Step 1: 4-[5-amino-6-[5-[3-(2-hydroxyethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide
[00449] To a solution of 4-[5-amino-6-[5-(3-vinylphenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2- yl]-N,N-dimethyl-benzamide (100 mg, 0.2425 mmol) in THF (9.172 mL) at 0°C, borane- THF complex (606.3 μL of 1 M, 0.6063 mmol) was added dropwise and the reaction mixture stirred overnight at room temperature. Water (43.69 mg, 43.69 μL, 2.425 mmol) was added to the reaction mixture followed by hydrogen peroxide (299.9 μL of 27.5 %w/v, 2.425 mmol) and NaOH (606.5 μL of 2 M, 1.213 mmol) and the mixture stirred vigorously for Ih. The mixture was partitioned between ethyl acetate (5 mL) and water (5 mL) and the layers separated. The aqueous layer was extracted further with ethyl acetate (2 x 5 mL) and combined organic extracts dried over MgSO 4 and concentrated in vacuo. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were combined and lyopholised to give the product as a yellow solid (5.8 mg, 11% yield); 1H NMR (400 MHz, DMSO) d 2.89 (t, 2H), 2.98 (m, 6H),
3.70 (q, 2H), 4.75 (t, 1H), 7.55-7.62 (m, 4H), 7.80 (br s, 2H), 8.01 (m, 2H), 8.17 (m, 2H) and
8.99 (s, 1 H) ppm; MS (ES+) 431.24
[00450] The following compounds were all prepared using a method similar to the one described for Compound IA-69 above.
Compound IA-275 4-[5-amino-6-[5-[3-(l-hydroxyethyl)phenyl]-1,3,4-oxadiazol-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzamide 1H NMR (400 MHz, DMSO) d 1.40 (d, 3H), 2.98
(m, 6H), 4.88 (m, I H), 5.46 (m, I H), 7.55 (m, 2H), 7.62 (m, 2H), 7.81 (br s, 2H), 8.03 (ni,
1H), 8.16 (m, 3H) and 8.99 (s, I H) ppm; MS (ES+) 431.24
Example 36A: 4-r5-amino-6-r5-f2-(3-thienvl)phenyπ-13,4-oxadiazol-2-yllpyrazin-2-yll- N,N-dimethyl-benzamide (Compound IA-127)
Figure imgf000271_0001
Compound IA-127
[00451] Compound IA- 127 was prepared using Method IV-B, Steps 1 -2, followed by Method IV-L, Step 1, followed by Method IV-AI, Step 1.
METHOD IV-AI
Step 1: 4-[5-amino-6-[5-[2-(3-thienyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]-N,N- dimethyl-benzamide
[00452] A solution of 4-[5-amino-6-[5-(2-bromophenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2- yl]-N,N-dimethyl-benzamide (50 mg, 0.108 mmol), thiophen-3-ylboronic acid (13.8 mg, 0.108 mmol), cesium carbonate (107 μL of 2M aqueous solution) and dichloropalladium; triphenylphosphane (7.55 mg, 0.0108 mmol) in dioxane (2 mL) was heated at 1 10 °C in the microwave for 1 h. The reaction mixture was cooled to room temperature and filtered. The filtrated was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were combined and lyopholised to give the product as a yellow solid (7.4 mg, 17% yield); I H NMR (400 MHz, DMSO) d 2.97 (m, 6H), 7.09 (m, I H), 7.53 (m, 2H), 7.60 (m, 2H), 7.64 (m, 2H), 7.71-7.76 (m, 3H), 8.01 (m, 2H), 8.07 (m, 1H) and 8.95 (s, I H) ppm; MS (ES+) 469.22
Example 37A: 3-r5-r3-(aminomethvOphenvπ-l .3.4-oxadiazol-2-vl')-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine (Compound IA-220)
SCHEME
Figure imgf000272_0001
Compound IA-220
[00453] Compound IA-220 was prepared using Method IV-AJ, Steps 1-4.
METHOD IV-AJ
Step 1: 3-amino-6-bromopyrazine-2-carbohydrazide
[00454] Methyl 3-amino-6-bromo-pyrazine-2-carboxylate (10.18 g, 43.87 mmol) was suspended in EtOH (50.90 mL) and hydrazine hydrate (4.392 g, 4.268 mL, 87.74 mmol) was added and the reaction mixture heated at 70 °C for 2 h. Water (50 mL) was added and the precipitate isolated by filtration. The solid was washed with methanol and dried under vacuum to leave the product as a pale yellow powder (9.8 g, 96 % yield); 1H NMR (400 MHz, DMSO) d 4.53 (bs s, 2H), 7.62 (br s, 2H) and 9.78 (br s, 1H) ppm; MS (ES+) 232.06
Step 2: ter/-butyl 3-(5-(3-amino-6-bromopyrazin-2-yl)-1,3,4-oxadiazol-2- yl)benzylcarbamate [00455] 3-amino-6-bromo-pyrazine-2-carbohydrazide (1.2 g, 5.172 mmol) , TBTU (1.333 g, 5.689 mmol), 3-[(tert-butoxycarbonylamino)methyl]benzoic acid (1.300 g, 5.172 mmol) and DIPEA (1.338 g, 1.803 mL, 10.35 mmol) in a solution in DMF (13.98 mL) were stirred at room temperature for 1 h. The reaction mixture was diluted with ethyl acetate (35 mL), washed with water (2 x 50 mL) and brine (1 x 50 mL). The organic layer was dried over MgSθ4 and concentrated in vacuo to a solid. This solid was suspended in MeCN (83.89 mL) at room temperature followed by the addition of dibromo(triphenyl)phosphorane (2.183 g, 5.172 mmol) and DIPEA (1.338 g, 1.803 mL, 10.35 mmol). The resulting mixture was stirred at room temperature for 2 h and then concentrated in vacuo to leave a solid. This was purified by column chromatography on silica eluting with EtOAc / petroleum ether, Product fractions were combined and concentrated in vacuo to leave the product as a white solid. The mixture was cone, to a solid and purified by column chromatography using ethylacetate/pet ether as elaunt to afford the product as a white solid (924 mg, 40% yield); 1H NMR (400 MHz, DMSO) d 1.43 (s, 9H), 4.26 (m, 2H), 7.55 (m, 3H), 7.80 (br s, 2H), 7.97 (m, I H) and 8.45 (s, 1H) ppm; MS (ES+) 449.08
Step 3: 3-[5-[3-(aminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5-(4-isopropylsulfonylphenyl) pyrazin-2-amine
[00456] Sodium carbonate (335.4 μL of 2 M, 0.6708 mmol) was added to a solution of tert-butyl N-[[3-[5-(3-amino-6-bromo-pyrazin-2-yl)-l ,3,4-oxadiazol-2- yl]phenyl]methyl]carbamate (100 mg, 0.2236 mmol), (4-isopropylsulfonylphenyl)boronic acid (66.30 mg, 0.2907 mmol), palladium; triphenylphosphane (25.84 mg, 0.02236 mmol) in dioxane (5 mL) and the resulting mixture heated at 1 1O°C under microwave conditions for 90 min. The mixture was placed directly onto silica gel pad and washed through with diethyl ether and followed by 50% EtOAc/ petroleum ether. Product fractions were combined and concentrated in vacuo. The residue was dissolved in CH2CI2 (10 mL) and TFA (764.9 mg, 516.8 μL, 6.708 mmol) was added. The reaction mixture was stirred at room temperature for 1 h and then concentrated in vacuo to leave an oil. This was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were combined and lyopholised to give the product as a yellow solid (36.05 mg, 35% yield); 1H NMR (400 MHz, DMSO) d 1.3 (d, 6H), 3.45-3.55 (m, I H), 4.24-4.3 (m,
2H), 7.7-7.8 (m, 2H), 7.95 (d, 2H), 8.25 (d, 1 H), 8.3-8.4 (br s, 2H), 8.4 (s, 1 H), 8.45 (d, 2H) and 9.1 (s, 1 H) ppm; MS (ES+) 451.2
[00457] The following compounds were all prepared using a method similar to the one described for Compound IA-220 above.
Compound IA-88 3-[4-[5-amino-6-[5-[4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2- yl]pyrazin-2-yl]phenyl]sulfonylbutan-1-ol 1H NMR (400 MHz, DMSO) d 1.21 (d, 3H), 1.38-
1.47 (m, 1H), 1.97-2.05 (m, 1H), 2.64 (t, 3H), 3.38-3.46 (m, 2H), 3.51-3.56 (m, 1H), 4.29 (t,
2H), 7.77 (d, 2H), 7.97-8.01 (m, 2H), 8.26 (d, 2H), 8.40-8.44 (m, 2H), 8.97 (s, 2H) and 9.09
(d, I H) ppm; MS (ES+) 495.0
Compound IA-257 3-[5-[3-(aminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5-(2- methylsulfinylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 3.1 (s, 3H), 4.3 (s, 2H), 7.6-7.75 (m, 4H), 8.05 (d, 1H), 8.15 (d, 1H), 8.35-8.5 (m, 4H) and 8.9 (s, 1H) ppm; MS (ES+) 407.1
Compound IA-321 5-(3-fluoro-4-isopropylsulfonyl-phenyl)-3-[5-[2-fluoro-4- (methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-arnine 1H NMR (400 MHz, DMSO) d 1.25 (d, 6H), 2.7 (s, 3H), 3.5-3.6 (m, 1H), 4.4 (s, 2H), 7.6 (d, 1H), 7.7 (d, 1H), 7.9 (t, I H), 8.2-8.3 (m, 2H), 8.35 (t, I H), 8.9-9.0 (br s, 2H) and 9.1 (s, I H) ppm; MS (ES+) 501.3
Compound IA-329 5-(3-chloro-4-isopropylsulfonyl-phenyl)-3-[5-[2-fluoro-4- (methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.25 (d, 6H), 2.7 (s, 3H), 3.7-3.8 (m, 1H), 4.4 (s, 2H), 7.6 (d, 1H), 7.7 (d, 1H), 8.1 (d, 1H), 8.25-8.35 (m, 2H), 8.4 (s, 1H), 8.9-9.0 (br s, 2H) and 9.1 (s, 1H) ppm; MS (ES+) 517.2
Compound IA-342 3-[4-[5-amino-6-[5-[2-fluoro-4-(methylaminomethyl)phenyl]-l ,3,4- oxadiazol-2-yl]pyrazin-2-yl]phenyl]sulfonylbutan-1-ol 1H NMR (400 MHz, DMSO) d 1.25 (d, 3H), 1.4-1.5 (m, I H), 1.95-2.03 (m, 1H), 2.7 (s, 3H), 3.4-3.5 (m, I H), 3.5-3.6 (m, 1H), 4.45 (s, 2H), 4.6-4.7 (m, 1H), 7.6 (d, 1H), 7.7 (d, I H), 8.0 (d, 2H), 8.3 (t, 1H), 8.4 (d, 2H), 9.0 (br s, 2H) and 9.1 (s, I H) ppm; MS (ES+) 513.2
Example 38A: 3-r5-r3-(dimethvlaminomethvπphenvll-1,3.4-oxadiazol-2-vll-5-('4- isopropylsulfonylphenyl)pyrazin-2-amine (Compound IA-204)
SCHEME
Figure imgf000275_0001
Compound IA-204
Compound IA-204 was prepared using Method IV-AJ, Steps 1 -3, followed by Method IV- AK, Step 1.
METHOD IV-AK
Step 1: 3-[5-[3-(dimethylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5,-(4- isopropylsulfonylphenyl)pyrazin-2-amine
[00458] 3-[5-[3-(aminomethyl)phenyl]- 1 ,3,4-oxadiazol-2-y l]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine (12 mg, 0.02108 mmol) was added to a solution of MeI (8.976 mg, 3.937 μL, 0.06324 mmol) and potassium carbonate (8.740 mg, 0.06324 mmol) in DMF (2 mL) . The resulting mixture was stirred at room temperature for 30 min. The reaction mixture was diluted with ethyl acetate (3 mL) and washed successively with water (1 x 5 mL) and brine (1 x 5 mL). The organic extracts were dried over MgSC^ and concentrated in vacuo. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were combined and lyopholised to give the product as a yellow solid (3.0 mg, 24 % yield); 1H NMR (400 MHz, MeOD) d 1.35-1.4 (m, 6H), 2.95 (s, 6H), 4.65 (s, 2H), 7.8-7.85 (m, 2H), 8.05-8.1 (m, 2H), 8.4-8.5 (m, 4H) and 9.95 (s, 1H) ppm; MS (ES+) 479.3
Example 39A: 5-(4-isopropvlsulfonvlphenvlV3-[5-(3-methvl-2-thienvlV1.3.4-oxadiazol-2- yl]pyrazin-2-amine (Compound IA-276)
SCHEME
IV-AL
Figure imgf000276_0001
Compound IA-276
Compound IA-276 was prepared using Method IV-AJ, Steps 1-3, followed by Method IV- AL, Step 1.
METHOD IV-AL: Step 1: 5-(4-isopropylsulfonylphenyl)-3-[5-(3-methyl-2-thienyl)-1,3,4- oxadiazol-2-yl]pyrazin-2-amine
[00459] A microwave vial was charged with 5-bromo-3-[5-(3-methyl-2-thienyl)- 1 ,3,4- oxadiazol-2-yl]pyrazin-2-amine (75 mg, 0.2218 mmol) , (4-isopropylsulfonylphenyl)boronic acid (50.59 mg, 0.2218 mmol), palladium; triphenylphosphane (12.82 mg, 0.01 109 mmol) and an aqueous sodium carbonate (332.7 μL of 2 M, 0.6654 mmol) solution was then added followed by DMF (1 mL) and the vial sealed. The reaction mixture was heated in the microwave at 150 °C for 30 min. After this time water was added and the resulting precipitate collected by filtration. The precipitate was passed through a palladium scavenging column eluting with MeCN and MeOH. The solvent was removed to give the product as a yellow solid (19.2 mg, 19 % yield); I H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 2.69 (s, 3H), 3.48 (t, I H)5 7.23 (d, I H), 7.92 (d, 2H), 7.98 (d, 2H), 8.36 - 8.34 (m, 2H) and 9.06 (s, 1 H) ppm; MS (ES+) 442.0 [00460] The following compounds were all prepared using a method similar to the one described for Compound IA-276 above.
Compound IA-269 5-[4-(2-dimethylaminoethylsulfonyl)phenyl]-3-[5-(3-methyl-2-thienyl)- l ,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 2.69 (s, 3H), 2.78 (s, 6H), 3.36 (s, 2H), 3.91-3.87 (m, 2H), 7.23 (d, 1H), 7.93 (d, 1H), 8.07 (d, 2H), 8.39 (d, 2H) and 9.08 (s, 1 H) ppm; MS (ES+) 471.0
Example 40A: [4-f5-r3-amino-6-r4-(dimethvlaminomethyl)phenvnpyrazin-2-y 11- 1,3,4- oxadiazol-2-yl]phenyl]methanol (Compound IA-240)
SCHEME
Method IV-C Method IV-R Method IV-AM
Figure imgf000277_0001
Compound IA-240
[00461] Compound IA-240 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1 , followed by Method IV-AM, Step 1.
METHOD IV-AM
Step 1: [4-[5-[3-amino-6-[4-(dimethylaminomethyl)phenyl]pyrazin-2-yl]-1,3,4-oxadiazol-2- yl]phenyl]methanol
[00462] Methyl 4-[5-[3-amino-6-[4-(dimethylcarbamoyl)phenyl]pyrazin-2-yl]-l, 3,4- oxadiazol-2-yl]benzoate (154 mg, 0.3465 mmol) in dry THF (1.5 mL) was cooled in ice-bath then treated dropwise with DIBAL (346.5 μL of 1 M solution in hexanes, 0.3465 mmol) . The resulting mixture was stirred 0-20 °C over 90 min and then at room temperature overnight. Additional DIBAL (1.732 mL of 1 M solution in hexanes, 1.732 mmol) was added at room temperature. The reaction mixture was poured onto water (10 mL) and acidified with 2M HCl, adjusted to pH 10 with aq NaOH solution and extracted EtOAc (6 x 10 mL) to give an orange solid. This was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were combined and passed through a bicarbonate cartridge. The eluent was concentrated in vacuo and taken up in acetonitrile and water and lyopholised to leave the product as a yellow powder (13.1 mg, 33 % yield); 1H NMR (400 MHz, DMSO) d 2.18 (s, 6H), 4.64 (s, 2H), 5.43 (br s, 1H), 4.43- 4.45 (m, 2H), 7.65-7.63 (m, 2H), 7.69 (br s, 2H), 8.05-8.30 (m, 4H) and 8.92 (s, 1H) ppm; MS (ES+) 403.18
Example 41 A: 4-r5-amino-6-r5-[4-(methvlaminomethvl)phenyll-1,3,4-oxadiazol-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzamide (Compound IA-281)
SCHEME
Figure imgf000278_0001
Compound IA-281
[00463] Compound IA-281 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1 , followed by Method IV-AN, Step 1.
METHOD IV AN
Step 1: 4-[5-amino-6-[5-[4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide
[00464] 4-[5-amino-6-[5-[4-(bromomethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide (70 mg, 0.1460 mmol) was treated with methylamine (2 mL of 33 %w/w solution in ethanol), and the resulting mixture stirred heated at 100 °C for 10 min. The reaction mixture was cooled to room temperature and filtered. The filtrate was collected and purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. The product fractions were passed througha carbonate cartridge leuting with MeOH/ CH2CI2. The eluent was concentrated in vacuo and the solid triturated with acetonitrile to give the product as a yellow solid (11.4 mg, 19 % yield); 1H NMR (400 MHz, DMSO) d 2.30 (s, 3H), 2.98-3.02(m, 6H), 3.77 (s, 2H), 7.55 (d, 2H), 7.61 (d, 2H), 7.78 (br s, 2H), 8.1 1 (d, 2H), 8.17 (d, 2H) and 8.98 (s, 1H) ppm; MS (ES+) 430.31
Example 42A: [4-r5-r3-amino-6-r4-(dimethvlcarbamovl)phenyllpvrazin-2-vπ-1,3,4- oxadiazol-2-yl]phenyl]methyl acetate (Compound IA-131)
SCHEME
Method IV-C NH2 Method IV-R Steps 1-2
Figure imgf000279_0001
Compound IA-131
[00465] Compound IA-131 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1, followed by Method IV-AO, Step 1.
METHOD IV-AO
Step 1: [4-[5-[3-amino-6-[4-(dimethylcarbamoyl)phenyl]pyrazin-2-yl]-l,3,4-oxadiazol-2- yl]phenyl]methyl acetate
[00466] A mixture of 4-[5-amino-6-[5-[4-(bromomethyl)phenyl]-l ,3,4-oxadiazol-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzamide (200 mg, 0.4172 mmol) and potassium acetate (102.4 mg, 1.043 mmol) in DMF (5.714 mL) was heated at 100 °C for 4 h in a sealed microwave tube. The reaction mixture was cooled to room temperature and poured onto ice/water and acidified by HCl (1.043 mL of 1 M, 1.043 mmol). The mixture was extracted with ethyl acetate (3 x 10 mL) and the combined organic extracts washed with brine (3 x 10 mL). The extracts were dried over MgSC>4 and concentrated under reduced pressure to give a yellow solid (150mg, 74% yield); 1H NMR (400 MHz, DMSO) d 2.13 (s, 3H), 2.98-3.02 (m, 6H), 5.22 (s, 2H), 7.55 (d, 2H), 7.66 (d, 2H), 7.78 (br s, 2H), 8.16-8.19 (m, 4H) and 8.99 (s, 1H) ppm; MS (ES+) 459.18
Example 43A: 4-r5-amino-6-[5-[4-(hvdroxvmethvOphenvll-1.3.4-oxadiazol-2-vπpyrazin-2- yl]-N,N-dimethyl-benzamide (Compound IA-76)
SCHEME
Figure imgf000280_0001
Compound IA-76
[00467] Compound IA-76 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1 , followed by Method IV-AO, Step 1, followed by Method IV-AP, Step 1.
METHOD IV-AP
Step 1: 4-[5-amino-6-[5-[4-(hydroxymethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N.N-dimethyl-benzamide [00468] [4-[5-[3-amino-6-[4-(dimethylcarbamoyl)phenyl]pyrazin-2-yl]-1,3,4-oxadiazol-2- yl]phenyl]methyl acetate (1 18 mg, 0.2445 mmol) was suspended in methanol (2 mL) and treated with NaOH (489.0 μL of 1 M, 0.4890 mmol). The resulting mixture was stirred at 55 °C for 1 h. The reaction mixture was cooled to room temperature and then neutralised with HCl (978.0 μL of 1 M, 0.9780 mmol), filtered and washed with acetonitrile. The resulting yellow powder was heated in acetonitrile (5 mL), cooled and filtered to give a pale yellow powder. This was purified by column chromatography on silica gel eluting with 5% MeOH/CH2Cl2 to give the product as a pale yellow powder (73 mg, 70%); 1H NMR (400 MHz, DMSO) d 2.98-3.02 (m, 6H), 4.64 (d, 2H), 5.44 (t, 1H), 7.54-7.62 (dd, 4H), 7.78 (br s, 2H), 8.12-8.18 (dd, 4H) and 8.98 (s, 1H) ppm; MS (ES+) 417.23
Example 44A: 4-r5-amino-6-r5-r4-(1.2-dihydroxyethyQphenvH-1.3.4-oxadiazol-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzamide (Compound IA-106)
SCHEME
Method IV-AQ Step i
Figure imgf000281_0001
Compound IA-106
[00469] Compound IA-106 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1 , followed by Method IV-AQ, Step 1.
METHOD IV-AQ
Step 1: 4-[5-amino-6-[5-[4-(1,2-dihydroxyethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]- N,N-dimethyl-benzamide
[00470] AD-mix-Alpha (450 mg, ) and methanesulfonamide (20.53 mg, 0.2158 mmol) in a mixture of t-butanol (2 mL)/ water (2 mL) were stirred at room temperature until dissolved, then cooled to 0 °C and treated with 4-[5-amino-6-[5-(4-vinylphenyl)-1,3,4-oxadiazol-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzamide (89 mg, 0.2158 mmol). The reaction mixture was stirred vigorously and warmed to room temperature overnight. A further portion of AD-mix (30Og) was added and the reaction mixture stirred overnight at room temperature to give complete conversion. The reaction mixture was treated with Na2SaCb /NaCl solution and extracted into ethyl acetate ( 10 mL), dried over MgSC>4 and concentrated under reduced pressure to give a yellow solid. This was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were combined and lyopholised to give the product as a pale yellow powder (28.4 mg, 36 % yield); 1H NMR (400 MHz, DMSO) d 2.9-3.02 (m, 6H), 3.51 (m, 2H), 4.67 (m, 1H), 4.83 (m, 1H), 5.49 (m, I H), 7.55 (d, 2H), 7.64 (d, 2H), 7.78 (br s, 2H), 8.1 1 (d, 2H), 8.18 (d, 2H) and 8.98 (s, 1H) ppm; MS (ES+) 447.25
Example 45A: 3-r5-r4-(aminomethvDphenvH-1.3.4-oxadiazol-2-vll-5-f4- isopropylsulfonylphenyl)pyrazin-2-amine (Compound IA-222)
SCHEME
IV-AR Method
Figure imgf000282_0001
Compound IA-222 Compound IA-222 was prepared using Method IV-C, Steps 1-2, followed by Method IV-AR, Steps 1-3.
METHOD IV-AR
Step 1: tert-buty\ 4-(2-(3-amino-6-(4-(isopropylsulfonyl)phenyI)pyrazine-2- carbonyl)hydrazinecarbonyl)benzylcarbamate
[00471] 3-amino-6-(4-isopropylsulfonylphenyl)pyrazine-2-carbohydrazide (100 mg, 0.2833 mmol) and 4-[(tert-butoxycarbonylamino)methyl]benzoic acid (71.19 mg, 0.2833 mmol) in dmf (1.000 mL) was treated with triethylamine (28.67 mg, 39.49 μL, 0.2833 mmol) followed by TBTU (109.2 mg, 0.3400 mmol) and the resulting solution stirred at room temperature overnight. The solution was poured dropwise onto rapidly stirred water (15ml), stirred at room temperature for 1 h and then filtered to give the product as a pale yellow solid which was dried under high vacuum at 83 °C and then used directly in the next step without further purification (136 mg, 84%)
Step 2: tert-buty\ 4-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)-l ,3,4- oxadiazol-2-yl)benzylcarbamate
[00472] A mixture of tert-buty\ 4-(2-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazine-2- carbonyl)hydrazinecarbonyl)benzylcarbamate(136 mg, 0.24 mmol) and DIPEA (109.8 mg, 148.0 μL, 0.8499 mmol) in acetonitrile (3.000 mL) at 0 °C was treated portionwise with dibromo(triphenyl)phosphorane (143.5 mg, 0.3400 mmol) and the resulting mixture stirred at room temperature for 48 h. . The mixture was concentrated in vacuo and pre-absorbed onto silica gel and purified by column chromatography on silica gel eluting with 50%EtOAc/CH2Cl2 to give the product (46.8 mg, 30 % yield); MS (ES+) 551.31
Step 3: 3-[5-[4-(aminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5-(4-isopropylsulfonylphenyl) pyrazin-2-amine
[00473] A solution of tert-butyl N-[[4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin- 2-yl]-l ,3,4-oxadiazol-2-yl]phenyl]methyl]carbamate (45 mg, 0.08172 mmol) in CH2Cl2 (1 mL) was treated with TFA (1 mL, 12.98 mmol) and stirred at room temperature for 1 h. The solution was concentrated under reduced pressure then azeotroped with methanol/ CH2CI2 (x2), dissolved in CH2Cl2ZMeOH and passed through a carbonate cartridge. The eluent was concentrated then crystallised from hot acetonitrile giving a yellow crystalline solid (18 mg, 41 % yield); 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 1.97 (br s, 2H), 3.47 (m, 1H), 3.85 (s, 2H), 7.63 (d, 2H), 7.89 (br s, 2H), 7.98 (d, 2H), 8.1 1 (d, 2H), 8.40 (d, 2H) and 9.06 (s, 1H) ppm; MS (ES+) 451.41
[00474] The following compounds were all prepared using a method similar to the one described for Compound IA-222 above.
Compound IA-80 3-[5-[3-[(l R)-I -aminoethyl]phenyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine I H NMR (400 MHz, DMSO) d 1.19 (m, 6H), 1.32 (m, 3H), 3.48 (m, 1H), 4.17 (m, 1H), 7.61 (m, 1H), 7.69 (m, I H), 7.99 (m, 5H), 8.19 (m, I H), 8.39 (m, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 465.32
Compound IA-84 5-(4-isopropylsulfonylphenyl)-3-[5-(3-pyrrolidin-2-ylphenyl)-1,3,4- oxadiazol-2-yl]pyrazin-2-amine 1HNMR (400 MHz, DMSO) d 1.19 (m, 6H), 1.57 (m, 1H), 1.80 (m, 2H), 2.20 (m, 1H), 2.97 (m, 1H), 3.01 (m, 1H), 3.45 (m, 1H), 4.24 (m, 1H), 7.59 (m, 1H), 7.67 (m, 1H), 7.97-8.03 (m, 4H), 8.19 (s, 1H), 8.39 (m, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 491.33
Compound IA-91 3-[5-(3-aminopropyl)-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.18-1.20 (m, 7H), 1.23 (s, 1H), 1.99 (t, 2H), 3.46 (t, 1H), 7.16 (s, 1H), 7.87 (d, 2H), 8.46 (d, 2H), 8.93 (s, 1H) and 10.20 (s, I H) ppm; MS (ES+) 403.23
Compound IA-92 3-[5-(4-aminobutyl)-1,3,4-oxadiazol-2-yl]-5-(4-isopropylsulfonylphenyl) pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.18-1.20 (m, 7H), 1.50 (t, 2H), 1.84 (t, 2H), 2.62 (t, 2H), 3.03 (t, 3H), 3.46 (t, 1H), 7.85 (br s, 1H), 7.96 (d, 2H), 8.31 (d, 2H) and 9.02 (s, 1 H) ppm; MS (ES+) 417.23
Compound IA-102 5-(4-isopropylsulfonylphenyl)-3-[5-(4-pyrrolidin-2-ylphenyl)-l ,3,4- oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 1.45-1.58 (m, 1H), 1.71-1.86 (m, 2H), 2.15-2.26 (m, 1H), 2.90-3.08 (m, 2H), 3.48 (m, 1H), 4.18 (t, 1H), 7.65 (d, 2H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.09 (d, 2H), 8.40 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 491.34
Compound IA-107 3-[5-[4-(2-aminoethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 2.79 (m, 2H), 2.85 (m, 2H), 3.47 (m, 1H), 7.51 (d, 2H), 7.92 (br s, 2H), 7.98 (d, 2H), 8.09 (d, 2H), 8.39 (d, 2H) and 9.06 (s, I H) ppm; MS (ES+) 465.34
Compound IA-123 3-[5-[3-[(lS)-1-aminoethyl]phenyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfony lphenyl)pyrazin-2-amine 1 H NMR (400 MHz, DMSO) d 1.19 (m, 6H), 1.32 (m, 3H), 3.49 (m, 1H), 4.18 (m, 1H), 7.61 (m, 1H), 7.69 (m, 1H), 7.99 (m, 4H), 8.19 (m, I H),
8.39 (m, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 465.32
Compound IA-124 3-[5-[4-[(l R)-I -aminoethyl]phenyl]-l, 3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 1.30 (d, 3H), 3.48 (m, I H), 4.1 1 (q, I H), 7.67 (d, 2H), 7.96 (v br s, 2H), 7.98 (d, 2H), 8.10 (d, 2H),
8.40 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 465.37
Compound IA-130 3-[5-[2-fluoro-4-(methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]-5- (4-isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.3 (d, 6H), 2.7 (s, 3H), 3.4-3.6 (m, I H), 4.35 (s, 2H), 7.6 (d, I H), 7.7 (d, 1H), 8.0 (d, 2H), 8.3 (t, 1H), 8.38 (d, 2H), 8.92 (br s, 2H) and 9.1 (s, 1H) ppm; MS (ES+) 483.4
Compound IA-145 5-(4-isopropylsulfonylphenyl)-3-[5-(l ,2,3,4-tetrahydroisoquinolin-6-yl)- 1,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.19 (m, 6H), 2.89 (m, 2H), 3.08 (m, 2H), 3.47 (m, 1H), 4.03 (s, 1H), 7.34 (m, 1H), 7.90-7.99 (m, 5H), 8.26 (s, 1H), 8.38 (m, 2H) and 9.03 (s, 1H) ppm; MS (ES+) 477.41
Compound IA-147 5-(4-isopropylsulfonylphenyl)-3-[5-(5-pyrrolidin-2-yl-2-thienyl)-1,3,4- oxadiazol-2-yl]pyrazin-2-amine I H NMR (400 MHz, DMSO) d 1.25 (d, 6H), 2.0-2.2 (m, 3H), 3.3-3.6 (m, 4H), 5.0-5.1 (m, I H), 7.9-8.0 (m, 4H), 8.4 (d, 2H), 9.05-9.1 (m, 2H) and 9.6 (br s, I H) ppm; MS (ES+) 497.4 Compound IA-168 3-[5-(aminomethyl)-1,3,4-oxadiazol-2-yl]-5-(4- isopropy lsulfonylpheny l)pyrazin-2-amine 1 H NMR (400 MHz, DMSO) d 1.16- 1.19 (m, 6H), 1.23 (s, 3H), 3.46 (t, 1H), 4.06 (s, 2H), 7.96 (d, 2H), 8.31 (d, 2H) and 9.02 (s, 1H) ppm; MS (ES+) 375.17
Compound IA-173 3-[5-[5-(aminomethyl)-2-thienyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 3.46- 3.51 (m, I H), 4.41 (s, 2H), 7.45 (d, 1H), 7.96 (d, 1H), 7.98 (d, 2H), 8.30 (br s, 2H), 8.37 (d, 2H) and 9.08 (s, 1H) ppm; MS (ES+) 457.3
Compound IA-185 3-[5-[3-(azetidin-3-yl)pheny I]-1 , 3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine I H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 3,48 (m, I H), 4.19 (m, 2H), 4.34 (m, 3H), 7.71-7.79 (m, 2H), 7.97 (m, 2H), 8.12 (m, 1H), 8.20 (m, 1H), 8.41 (m, 2H), 8.69 (br s, I H) and 9.09 (s, I H) ppm; MS (ES+) 477.29
Compound IA-201 3-[5-(4-aminophenyl)-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.21 (d, 6H), 347 (m, 1H), 6.10 (s, 2H), 6.75 (d, 2H), 7.83 (d, 2H), 7.89 (br s, 2H), 7.97 (d, 2H), 8.39 (d, 2H) and 9.02 (s, 1H) ppm; MS (ES+) 437.22
Compound IA-214 3-[5-(2-aminoethyl)-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.19 (d, 6H), 3.05 (m, 4H), 3.33 (d, 1H), 3.46 (s, I H), 7.96 (d, 2H), 8.31 (d, 2H) and 9.02 (s, 1H) ppm; MS (ES+) 389.24
Compound IA-228 3-[5-[4-[(lS)-1-aminoethyl]phenyl]-l ,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 1.30 (d, 3H), 3.48 (m, I H), 4.1 1 (q, I H), 7.67 (d, 2H), 7.96 (v br s, 2H), 7.98 (d, 2H), 8.10 (d, 2H), 8.40 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 465.42 .
Compound IA-232 5-(4-isopropylsulfonylphenyl)-3-[5-(l ,2,3,6-tetrahydropyridin-4-yl)- 1,3,4-oxadiazol-2-yl]pyrazin-2-amine I H NMR (400 MHz, DMSO) d 1.19 (d, 6H), 2.50 (s, 2H), 2.98 (br t, 2H), 3.46 (m, 1H), 3.55 (dr d, 2H), 7.03 (s, 1H), 7.90 (br s, 2H), 7.95 (d, 2H), 8.35 (d, 2H) and 9.04 (s, 1H) ppm; MS (ES+) 427.4
Compound IA-282 3-[5-[3-(l-aminoethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (m, 6H), 1.33 (m, 3H), 3.48 (m, I H), 4.18 (m, 1H), 7.61 (m, I H), 7.70 (m, 1 H), 7.98-8.03 (m, 4H), 8.20 (m, 1H), 8.39 (m, 2H) and 9.07 (s, I H) ppm; MS (ES+) 465.3
Compound IA-285 3-[5-[4-(azetidin-3-yl)phenyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 3.48 (m, 1H), 3.65 (m, 2H), 3.87 (m, 2H), 3.99 (m, 1H), 7.65 (d, 2H), 7.91 (br s, 2H), 7.98 (d, 2H), 8.14 (d, 2H), 8.39 (d, 2H) and 9.06 (s, 1H) ppm; MS (ES+) 477.44
Compound IA-306 3-[5-[2-chloro-4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5- (4-isopropylsulfonylphenyl)pyrazin-2-amine MS (ES+) 499.2
Compound IA-309 3-[5-[3-chloro-4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5- (4-isopropylsulfonylphenyl)pyrazin-2-amine 1 H NMR (400 MHz, DMSO) d 1.25 (d, 6H), 2.7 (s, 3H), 3.4-3.5 (m, 1 H),4.45 (s, 2H), 7.7 (d, 1H), 8.0 (d, 2H), 8.3 (d, I H), 8.4 (d, 2H), 9.0 (br s, 2H) and 9.1 (s, 1H) ppm; MS (ES+) 499.2
Compound IA-311 3-[5-[4-(l -amino- 1-methy l-ethyl)phenyl]-l , 3,4-oxadiazoI-2-y l]-5-(4- isopropylsulfonylpheny l)pyrazin-2-amine 1 H NMR (400 MHz, DMSO) d 1.19 (m, 6H), 1.43 (s, 6H), 3.51 (m, I H), 7.83 (m, 2H), 7.97 (m, 2H), 8.09 (m, 2H), 8.39 (m, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 479.27
Example 46A: 2-15-r3-amino-6-(4-isopropvlsulfonvlphenyl)pyrazin-2-vl'|-l .3.4-oxadiazol-2- yljphenol (Compound IA-235)
SCHEME
Figure imgf000288_0001
Compound IA-235
Compound IA-235 was prepared using Method IV-C, Steps 1-2, followed by Method IV-X, Steps 1-2, followed by Method IV-AS, Step 1.
METHOD IV-AS
Step 1: 2-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-l ,3,4-oxadiazol-2- yl]phenol
[00475] LiOH (292.0 μL of 1 M, 0.2920 mmol) was added to a suspension of 2-(5-(3- amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)-l ,3,4-oxadiazol-2-yl)phenyl acetate (14 mg, 0.02920 mmol) in THF (5 mL) at ambient temperature. After 3 h, a further portion of LiOH (292.0 μL of 1 M, 0.2920 mmol) was added and the reaction continued to stir at room temperaure for 1 h. IM HCl was added dropwise until the reaction mixture was acidic and the resultant precipitate isolated by filtration. The solid residue was dissolved in a mixture of MeCN and water and lyopholised to give the product as a green solid (5.1 mg, 38 % yield); I H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 3.46 (m, 1H), 6.98-7.22 (m, 2H), 7.36-7.59 (m, 1H), 7.75-8.16 (m, 5H), 8.37 (d, 2H), 9.06 (s, I H) and 10.43 (s, I H) ppm; MS (ES+) 438.2 [00476] The following compounds were all prepared using a method similar to the one described for Compound IA-235 above.
Compound IA-193 4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-l ,3,4- oxadiazol-2-yl]phenol 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 3.47 (m, 1H), 7.03 (dd, 2H), 7.89 (br s, 2H), 7.97 (dd, 2H), 8.02 (dd, 2H), 8.39 (dd, 2H), 9.04 (s, 1H) and 10.44 (s, I H) ppm; MS (ES+) 438.2
Example 47A: 5-(4-isopropvlsulfonvlphenvl)-3-[5-r4-(methvlaminomethvl')phenvll- 1,3,4- oxadiazol-2-yl]pyrazin-2-amine (Compound IA-159)
SCHEME
Figure imgf000289_0001
Compound IA-159
[00477] Compound IA-159 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1 , followed by Method IV-AT, Step 1.
METHOD IV-AT
Step 1: 5-(4-isopropylsuIfonylphenyl)-3-[5-[4-(methylaminomethyl) phenyl]- 1,3,4- oxadiazol-2-yl]pyrazin-2-amine
[00478] MeNH2 in ethanol (184.9 g, 243.6 mL of 33 %w/w, 1.965 mol) was added in one portion to a stirred solution of 3-[5-[4-(bromomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine (10.11 g, 19.65 mmol) in CH2CI2 (1.01 L) and methanol (1.01 L) and the resulting mixture stirred overnight at room temperature. Nitrogen was bubbled through reaction for 2 h and then the reaction mixture was concentrated in vacuo. The crude material was stirred in K2CO3 (393.0 mL of 0.25 M, 98.25 mmol) for 2 h and then isolated by filtration and washed with water. Triturated with warm acetonitrile to leave the product as a yellow solid (7.19 g, 75 % yield); 1H NMR (400 MHz, DMSO) d 1.19-1.21 (d, 6H), 2.30 (m, 3H), 3.35-3.49 (m, 1H), 3.77 (m, 2H), 7.61-7.63 (d, 2H), 7.97- 7.99 (d, 2H), 8.11-8.13 (d, 2H), 8.39-8.41 (d, 2H) and 9.06 (s, 1H) ppm; MS (ES+) 465.4 [00479] The following compounds were all prepared using a method similar to the one described for Compound IA-159 above.
Compound IA-119 5-(4-isopropylsulfonylphenyl)-3-[5-[4-[(2-methoxyethylamino) methyl]phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-amine 1 H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 2.67 (t, 2H), 3.25 (s, 3H), 3.43 (t, 2H), 3.48 (m, I H), 3.84 (s, 2H), 7.62 (d, 2H), 7.97 (d, 2H), 7.98 (v br s, 2H), 8.12 (d, 2H), 8.40 (d, 2H) and 9.07 (s, I H) ppm; MS (ES+) 509.37 Compound IA-122 3-[5-[4-(ethylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylpheny l)pyrazin-2-amine 1 H NMR (400 MHz, DMSO) d 1.17- 1.22 (m, 9H), 2.89 (q, 2H), 3.48 (m, 1H), 4.15 (s, 2H), 7.77 (d, 2H), 7.98 (d, 2H), 7.99 (br s, 2H), 8.21 (d, 2H), 8.41 (d, 2H) and 9.08 (s, I H) ppm; MS (ES+) 479.41
Compound IA-139 2-[[4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-l ,3,4- oxadiazol-2-yl]phenyl]methylamino]-2-(hydroxymethyl)propane-1,3-diol I H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 3.44 (s, 6H), 3.45 (m, 1H), 3.90 (s, 2H), 4.37 (br s, 3H), 7.66 (d, 2H), 7.95 (br s, 2H), 7.98 (d, 2H), 8.1 1 (d, 2H), 8.40 (d, 2H) and 9.07 (s, I H) ppm; MS (ES+) 555.32
Compound IA-146 5-(4-isopropylsulfonylphenyl)-3-[5-[3-(methylaminomethyl)phenyl]- l ,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.19 (d, 6H), 2.33 (s, 3H), 3.48 (m, 1H), 3.83 (s, 2H), 7.61-7.67 (m, 2H), 7.97 (m, 3H), 8.05 (m, 1H), 8.15 (m, 1H), 8.39 (m, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 465.29
Compound IA-158 3-[5-[4-[(cyclopropylamino)methyl]phenyl]-1,3,4-oxadiazol-2-yI]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 0.28 (m, 2H), 0.36 (m, 2H), 1.20 (d, 6H), 2.07 (m, I H), 3.48 (m, 1H), 3.85 (s* 2H), 7.61 (d, 2H), 7.96 (br s, 2H), 7.98 (d, 2H), 8.1 1 (d, 2H), 8.40 (d, 2H) and 9.07 (s, 1 H) ppm; MS (ES+) 491.42 Compound IA-178 2-(4-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-y I)- 1 ,3,4- oxadiazol-2-yl)benzylamino)ethanol 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 2.61 (t, 2H), 3.42-3.51 (m, 3H), 3.86 (s, 2H), 4.54 (br s, 1 H), 7.63 (d, 2H), 7.80 (br s, 2H), 7.98 (d, 2H), 8.12 (d, 2H), 8.39 (d, 2H) and 9.06 (s, 1H) ppm; MS (ES+) 495.31 Compound IA-225 N-[[4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4- oxadiazol-2-yl]phenyl]methyl]-N',N'-dimethyl-ethane-1,2-diamine I H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 3.07 (s, 3H), 3.29-3.49 (m, 5H), 4.74 (s, 2H), 7.91 (d, 2H), 7.97 (d, 2H), 7.98 (v br s, 2H), 8.27 (d, 2H), 8.40 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 522.23 Compound IA-238 [4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-l ,3,4- oxadiazol-2-yl]phenyl]methanol I H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 3.48 (m, 1H), 6.64 (d, 2H), 5.46 (t, I H), 7.61 (d, 2H), 7.98 (d, 2H), 7.99 (br s, 2H), 8.15 (d, 2H), 8.40 (d, 2H) and 9.07 (s, I H) ppm; MS (ES+) 452.26
Compound IA-243 3-[5-[4-(dimethylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (m, 6H), 2.21 (s, 6H), 3.47 (m, 1H), 3.54 (s, 2H), 7.59 (d, 2H),'7.90 (br s, 2H), 7.97 (d, 2H), 8.13 (d, 2H), 8.39 (d, 2H) and 9.06 (s, 1H) ppm; MS (ES+) 479.37
Compound IA-333 (Λ)-2-(4-(5-(3-amino-6-(4-(isopropylsuIfonyl)phenyl)pyrazin-2-yl)- 1,3,4-oxadiazol-2-yl)-3-fluorobenzylamino)propan-1-ol 1H NMR (400 MHz, DMSO) d 1.06 (3H, d), 1.20 (d, 6H), 2.44 (m, 1H), 3.35 (obscured, 2H), 3.48 (m, 1H), 3.85 (m, 2H), 4.53 (m, 1H), 7.47 (d, 1H), 7.52 (d, I H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.12 (t, I H), 8.37 (d, 2H) and 9.08 (s, 1H) ppm; MS (ES+) 527.2
Compound IA-334 (S)-1-(4-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)- 1,3,4-oxadiazol-2-yl)-3-fluorobenzylamino)propan-2-ol 1H NMR (400 MHz, DMSO) d 1.06 (d, 3H), 1.20 (d, 6H), 3.45 (m, I H), 3.71 (m, I H), 4.53 (d, 1H), 7.46 (d, I H), 7.52 (d, 1H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.12 (t, 1H), 8.37 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 527.2 Compound IA-335 (S)-2-(4-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)- 1,3,4-oxadiazol-2-yl)-3-fluorobenzylamino)propan-1-ol 1H NMR (400 MHz, DMSO) d 0.97 (d, 3H), 1.20 (d, 6H), 2.62 (m, 1H), 3.30 (m, 2H), 3.48 (m, 1H), 3.88 (m, 2H), 4.58 (m, 1H), 7.47 (d, 1H), 7.53 (d, 1H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.12 (t, 1H), 8.37 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 527.2
Compound IA-336 3-[5-[3-fluoro-4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5- (4-isopropylsulfonylphenyl)pyrazin-2-amine 1 H NMR (400 MHz, DMSO) d 1.20 (m, 6H), 2.68 (m, 3H), 3.48 (m, I H), 4.34 (m, 2H), 7.86 (t, 1H), 7.99 (m, 2H), 8.09 (m, 1H), 8.14 (dd, 1H), 8.42 (m, 2H), 8.96 (br s, 2H) and 9.1 1 (s, 1H) ppm; MS (ES+) 483.1 Compound IA-340 3-[5-[2-fluoro-4-[(2-fluoroethylamino)methyl]phenyl]-l ,3,4-oxadiazol- 2-yl]-5-(4-isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 2.81 (m, 2H), 3.48 (m, I H), 3.88 (s, 2H), 4.51 (m, 2H), 7.47 (d, 1H), 7.52 (d, I H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.12 (t, 1 H), 8.37 (d, 2H) and 9.07 (s, 1 H) ppm; MS (ES+) 515.2 Compound IA-341 (R)-1-(4-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)- 1,3,4-oxadiazol-2-yl)-3-fluorobenzylamino)propan-2-ol 1H NMR (400 MHz, DMSO) d 1.06 (dd, 3H), 1.20 (dd, 6H), 3.45 (m, 1H), 3.71 (m, 1H), 4.53 (m, 1H), 7.46 (d, 1H), 7.52 (d, 1H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.12 (t, 1H), 8.37 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 527.2 Compound IA-345 3-[5-[2-fluoro-4-[(tetrahydrofuran-3-ylamino)methyl]phenyl]- 1,3,4- oxadiazol-2-yl]-5-(4-isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 1.70-1.74 (m, 1H), 1.90-1.98 (m, 1H), 3.25-3.32 (m, 1H), 3.41-3.50 (m, 2H), 3.60-3.85 (m, 5H), 7.47 (d, 1H), 7.53 (d, 1H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.12 (t, 1H), 8.37 (d, 2H) and 9.08 (s, I H) ppm; MS (ES+) 539.3
Compound IA-346 3-[5-[4-[(2-fluoroethylamino)methyl]phenyl]-l ,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 2.82 (m, 2H), 3.48 (m, 1H), 3.87 (s, 2H), 4.50 (m, 2H), 7.64 (d, 2H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.13 (d, 2H), 8.40 (d, 2H) and 9.06 (s, 1H) ppm; MS (ES+) 497.2
Compound IA-348 l-(4-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)-l ,3,4- oxadiazol-2-yl)-3-fluorobenzylamino)-2-methylpropan-2-ol 1 H NMR (400 MHz, DMSO) d 1.13 (s, 6H), 1.20 (d, 6H), 2.39 (s, 2H), 3.48 (m, 1H), 3.88 (s, 2H), 4.27 (s, 1H), 7.45 (d, 1H), 7.51 (d, 1H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.12 (t, 1H), 8.37 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 541.2
Compound IA-319 3-[5-[2-fluoro-4-[(oxetan-3-ylamino)methyl]phenyl]-1,3,4-oxadiazol-2- yl]-5-(4-isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 4.48 (m, 1H), 3.77 (s, 2H), 3.80 (m, 1H), 4.34 (t, 2H), 4.59 (t, 2H), 7.45 (d, 1H), 7.51 (d, I H), 7.97 (br s, 2H), 7.98 (d, 2H), 8.12 (t, 1H), 8.37 (d, 2H) and 9.07 (s, I H) ppm; MS (ES+) 525.2
Example 48A: 4-r5-r3-amino-6-(4-isopropylsulfonvlphenv0pyrazin-2-vl-|-l .3.4-oxadiazol-2- yl]benzamidine (Compound IA-70)
SCHEME IV-AU
Figure imgf000293_0001
Compound IA-70
Compound IA-70 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1, followed by Method IV-AU, Step 1
METHOD IV-AU
Step 1: 4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazol-2- yl]benzamidine
[00480] 4-[5-[3-amino-6-(4-isopropylsulfony lphenyl)pyrazin-2-yl]- 1 ,3,4-oxadiazol-2- yl]benzonitrile (58 mg, 0.1299 mmol) was suspended in a mixture OfCH2Cl2 (3 mL) / ethanol (4 mL, 68.51 mmol), sonicated then stirred at O°C during the addition of HCl gas until saturated. The resulting suspension was stirred at room temperature for 4 h, then warmed to 40 °C and stirred overnight. The mixture was concentrated to dryness under reduced pressure then suspended in absolute ethanol (60ml), cooled in ice bath and ammonia gas bubbled through for 5 min. The reaction vessel was sealed and then stirred at room temperature for 3 h, then heated at 50°C overnight. The reaction mixture was concentrated in vacuo and purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were combined and lyopholised to give the product as a pale yellow powder (16.7 mg, 32 % yield); 1H NMR (400 MHz, DMSO) d 1.21 (d, 6H), 3.48 (m, I H), 6.80 (br s, 2H), 7.98 (d, 2H), 8.05 (d, 2H), 8.21 (d, 2H), 8.41 (d, 2H) and 9.08 (s, 1H) ppm; MS (ES+) 464.24 [00481] The following compounds were all prepared using a method similar to the one described for Compound IA-70 above.
Compound IA-208 4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- 1,3,4- oxadiazol-2-yl]-N-methyl-benzamidine 1H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 2.88 (s, 3H), 3.48 (m, I H), 6.83 (br s, 1H), 7.98 (br s, 2H), 7.99 (d, 2H), 8.03 (d, 2H) 8.19 (d, 2H), 8.41 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 478.24
Example 49A: 4-r5-r3-amino-6-r4-C2-dimethvlaminoethvlsulfonvl')phenvllpvrazin-2-vn- l ,3,4-oxadiazol-2-yl]phenol (Compound IA-191)
SCHEME
IV-AV
Figure imgf000294_0001
Compound IA-191
Compound IA-191 was prepared using Method IV-AJ, Steps 1-2, followed by Method IV- AV, Step 1.
METHOD IV-AV
Step 1: 4-[5-[3-amino-6-[4-(2-dimethylaminoethylsulfonyl)phenyl]pyrazin-2-yl]-l ,3,4- oxadiazol-2-yl]phenol
[00482] 2-(4-bromophenyl)sulfonyl-N,N-dimethyl-ethanamine (181.8 mg, 0.6221 mmol) was dissolved in dioxane (2 mL) and bis(pinacolato)diboron (237.0 mg, 0.9332 mmol) and potassium acetate (183.1 mg, 1.866 mmol) were added. The reaction mixture was degassed and filled with nitrogen (5x) then Pd(dppf)Cl2.CH2Cl2 (50.80 mg, 0.06221 mmol) was added and the reaction heated to 90 °C for 2 hours. The reaction mixture was cooled to ambient temperature and diluted with DMF (2 mL). [4-[5-(3-amino-6-bromo-pyrazin-2-yl)- 1,3,4- oxadiazol-2-yl]phenyl] acetate (234 mg, 0.6221 mmol), Na2CO3 (933.0 μL of 2 M aqueous solution, 1.866 mmol) and Pd(PPh3)2Cl2 (43.67 mg, 0.06221 mmol) were added and the reaction heated at 150 °C under microwave conditions for 30 minutes. The reaction mixture was partitioned between EtOAc (5 mL) and water (5 mL) and any precipitate removed by filtration. The layers were separated and the aqueous layer extracted with EtOAc (3 x 5 mL) and the combined organic extracts dried over MgSθ4, filtered and concentrated in vacuo. The residue was tritruated form EtOAc/MeOH to give the title compound as a brown solid (44.3 mg, 15 %); 1H NMR (400 MHz, DMSO) d 2.07 (s, 6H), 2.56 (t, 2H), 3.52 (t, 2H), 7.03 (d, 2H), 7.87 (br s, 2H), 8.02 (dd, 4H), 8.38 (d, 2H), 9.05 (s, 1H) and 10.44 (s, 1H) ppm; MS (ES+) 467.2
Example 5OA: 5-r3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yll-1,3,4-oxadiazol-2- amine (Compound IA-270)
SCHEME
NH2 IV-AW K,
Figure imgf000295_0001
Compound IA-270
Compound IA-270 was prepared using Method IV-B, Steps 1-2, followed by Method IV- AW, Steps 1-2.
METHOD IV-AW
Step 1: 2-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazine-2- carbonyl)hydrazinecarbothioamide
[00483] TBTU (749.4 mg, 2.334 mmol) and Et3N (157.5 mg, 216.9 μL, 1.556 mmol) were added to a suspension of 3-amino-6-(4-isopropylsulfonylphenyl)pyrazine-2-carboxylic acid (500 mg, 1.556 mmol) and aminothiourea (141.8 mg, 1.556 mmol) in DMF (10 mL) . The reaction was allowed to stir at ambient temperatuer for 1 h. The reaction mixture was added to rapidly stirring water and the resultant precipitate isolated by filtration to give the product as a khaki solid (587 mg, 96%) 1H NMR (400 MHz, DMSO) d 1.18 (d, 6H), 3.40-3.56 (m, 1H), 7.64 (s, 2H), 7.79 (s, 2H), 7.88 (d, 2H), 8.56 (d, 1H), 9.03 (s, 1H), 9.41 (s, 1H) and 10.75 (s, I H) ppm; MS (ES+) 395.2
Step 2: 5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-l ,3,4-oxadiazol-2-amine
[00484] EDC (109.3 mg, 0.5704 mmol) was added to a stirred suspension of [[3-amino-6- (4-isopropylsulfonylphenyl)pyrazine-2-carbonyl]amino]thiourea (150 mg, 0.3803 mmol) in DCE (3.000 mL) and the reaction mixture heated at reflux for 22 h. The solvent was removed in vacuo and the residue partitioned between EtOAc and water. The aqueous layer was extratced with EtOAc (3 x 10 mL) and the combined organic extracts dried MgSO4, filtered and concentrated in vacuo to give the sub-title compound as a yellow solid (118 mg, 86%) 1H NMR (400 MHz, DMSO) d 1.19 (d, 6H), 3.45 (dt, 1H), 7.65-7.80 (m, 4H), 7.95 (d, 2H), 8.26 (d, 2H) and 8.89 (s, 1H) ppm; MS (ES+) 361.0
Example 51: 3-r5-f5-(ethylaminomethvl')-2-thienvll-1.3.4-oxadiazol-2-vll-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine (Compound IA-83)
SCHEME x! X A )■
Figure imgf000297_0001
Compound IA-83
[00485] Compound IA-83 was prepared using Method IV-C, Steps 1-2, followed by Method IV-X, Steps 1-2, followed by Method IV-AX, Steps 1-3.
METHOD IV-AX
Step 1: di-tert-butyl N-[5-(4-isopropylsulfonylphenyl)-3-[5-(5-methyl-2-thienyl)-1,3,4- oxadiazol-2-yl]pyrazin-2-yl]iminodicarbonate
[00486] 5-(4-isopropylsulfonylphenyl)-3-[5-(5-methyl-2-thienyl)-1,3,4-oxadiazol-2- yl]pyrazin-2-amine (600 mg, 1.359 mmol) was added to MeCN (50 mL) followed by the addition OfBOC2O (889.8 mg, 936.6 μL, 4.077 mmol) and DMAP (8.301 mg, 0.06795 mmol). The resulting mixture was stirred overnight at room temperature. The reaction mixture was concentrated in vacuo to leave a solid which was purified by column chromatography on silica gel eluting with 50% EtOAc/ petroleum ether (544.6 mg, 74%) 1H NMR (400 MHz, CDCl3) d 1.29 (d, 6H), 1.36 (s, 9H), 2.54 (s, 3H, 3.20 (m, 1H), 6.83 (m, 1 H), 7.71 (m, 1 H), 8.03 (m, 2H), 8.31 (m, 2H) and 9.06 (s, 1 H) ppm Step 2: di-ter/-butyl 3-(5-(5-(bromomethyl)thiophen-2-yl)-l ,3,4-oxadiazol-2-yl)-5-(4- (isopropylsulfonyl)phenyl)pyrazin-2-yliminodicarbonate
[00487] To a solution of di-tert-butyl N-[5-(4-isopropylsulfonylphenyl)-3-[5-(5-methyl-2- thienyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]iminodicarbonate (700 mg, 1.292 mmol) in ethyl acetate (50 mL) was added NBS (299.0 mg, 1.680 mmol) and AIBN (42.43 mg, 0.2584 mmol). The resulting mixture was heated to reflux for 2 h. The reaction mixture was cooled to room temperature and filtered, washed with water and the organic layer was dried over MgSθ4 and concentrated in vacuo to a yellow solid which was used in the next stage without further purification.
Step 3: 3-[5-[5-(ethylaminomethyl)-2-thienyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine
[00488] To a solution of di-tert-butyl 3-(5-(5-(bromomethyl)thiophen-2-yl)-1,3,4- oxadiazol-2-yl)-5-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yliminodicarbonate (90 mg, 0.1450 mmol) in ethanol (2 mL) at room temperature was added ethylamine (7.250 mL of 2 M in ethanol, 14.50 mmol). The resulting mixture was stirred at room temperature I h. The mixture was concentrated in vacuo to leave a solid. The solid was redissolved in CH2CI2 and concentrated to a solid to remove any remaining methanol. The solid was dissolved in CH2Cl2 (3 mL) and TFA (165.3 mg, 1 1 1.7 μL, 1.450 mmol) was added. The mixture was stirred at room temperature for 2 h and then concentrated in vacuo and the residue purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. Product fractions were combined and lyopholised to give the product as a pale yellow powder (63 mg, 73.5 %); 1H NMR (400 MHz, DMSO) d 1.20-1.25 (m, 9H), 3.0-3.1 (m, 2H), 3.42-3.46 (m, 1H), 4.5 (s, 2H), 7.5 (d, 1H), 7.95 (d, I H), 8.01 (d, 1H), 8.38 (d, 1H), 9.0 (br s, 2H) and 9.18 (s, 1H) ppm; MS (ES+) 485.4
[00489] The following compounds were all prepared using a method similar to the one described for Compound IA-83 above. Compound IA-140 5-(4-isopropylsulfonylphenyl)-3-[5-[5-(methylaminomethyl)-2-thienyl]- l ,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.22 (d, 6H), 2.65 (s, 3H), 3.42-3.46 (m, 1H), 4.5 (s, 2H), 7.5 (d, 1H), 8.0 (d, 1H), 8.05 (d, 1H), 8.4 (d, 1H), 9.05 (br s, 2H) and 9.1 (s, 1H) ppm; MS (ES+) 471.3
Compound IA-226 5-(4-isopropylsulfonylphenyl)-3-[5-[4-(methylaminomethyl)-2-thienyl]- l ,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400 MHz, MeOD) d 1.4 (d, 6H), 2.8 (s, 3H), 4.4 (s, 2H), 3.3-3.4 (m, 1H), 8.0-8.1 (m, 3H), 8.12 (s, 1H), 8.35 (d, 2H) and 9.0 (s, I H) ppm; MS (ES+) 471.3
Compound IA-236 3-[5-[5-[(2,2-difluoroethylamino)methyl]-2-thienyl]-1,3,4-oxadiazol-2- yl]-5-(4-isopropylsulfonylphenyl)pyrazin-2-amine 1HNMR (400 MHz, DMSO) d 1.25 (d, 6H), 3.4-3.6 (m, 3H), 4.55 (s, 2H), 6.2-6.5 (m, 1H), 7.5 (d, 1H), 7.8-8.1 (m, 4H), 8.45 (d, 2H) and 9.1 (s, 1 H) ppm; MS (ES+) 521.3
Compound IA-248 3-[5-[5-[(isopropylamino)methyl]-2-thienyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsu!fonylphenyl)pyrazin-2-amine 1 H NMR (400 MHz, DMSO) d 1.25 (m, 6H), 1.35 (d, 6H), 3.4-3.6 (m, 2H), 4.6 (s, 2H), 7.5 (d, 1H), 7.95-8.1 (m, 4H), 8.45 (d, 2H), 8.9-9.0 (br s, 2H) and 9.1 (s, 1H) ppm; MS (ES+) 499.4
Example 52A: N-r5-r3-amino-6-(4-isopropvlsulfonylphenyl)pyrazin-2-yl1-l ,3,4-oxadiazol- 2-yl]acetamide (Compound IA-177)
SCHEME
Figure imgf000299_0001
Compound IA-177 Compound IA- 177 was prepared using Method IV-C, Steps 1-2, followed by Method IV-AY, Steps 1-2.
METHOD IV-AY
Step 1: N-(2-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazine-2-carbonyl) hydrazinecarbonothioyl)ethanamide
[00490] A mixture of 3-amino-6-(4-isopropylsulfonylphenyl)pyrazine-2-carbohydrazide (100 mg, 0.2982 mmol) , acetyl isothiocyanate (30.16 mg, 26.20 μL, 0.2982 mmol) and dry DCE (2.000 mL) were stirred at ambient temperature for 2 h and then concentrated in vacuo. Used directly in the next step without further purification; MS (ES+) 437.20
Step 2: Ν-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazol-2- yl]acetamide
[00491] N-(2-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazine-2- carbonyl)hydrazinecarbonothioyl) acetamide (47 mg, 0.1077 mmol) was dissolved in DMF (2 mL) and EDC (30.98 mg, 0.1616 mmol) was added. The reaction was allowed to stir at ambient temperature for 45 minutes then warmed to 100 °C for 1 hour. The reaction mixture was cooled to ambient temperature then added slowly to stirred water. The resultant precipitate was isolated by filtration to give the sub-title product as a yellow solid (31 mg, 68%); 1 H NMR (400 MHz, DMSO) d 1.18 (d, 6H), 2.20 (s, 3H), 3.41-3.49 (m, I H), 7.81 (br s, 2H), 8.14 (d, 2H), 8.27 (d, 2H), 8.99 (s, 1 H) and 1 1.98 (s, 1 H) ppm; MS (ES+) 403.2
Example 53A: 2-amino-N-r5-r3-amino-6-(4-isopropylsulfonvlphenvπpvrazin-2-vll-l .3.4- oxadiazol-2-yl]acetamide (Compound IA-82)
SCHEME
Figure imgf000301_0001
Compound IA-82
[00492] Compound IA-82 was prepared using Method IV-C, Steps 1-2, followed by Method IV-AY, Steps 1-2, followed by Method IV-AZ, Step 1.
METHOD IV-AZ
Step 1: 2-amino-N-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-l ,3,4-oxadiazol- 2-yl]acetamide
[00493] Hydrazine hydrate (8.065 mg, 7.838 μL, 0.1611 mmol) was added to a stirred suspension ofN-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)-l ,3,4-oxadiazol- 2-yl)-2-(l ,3-dioxoisoindolin-2-yl)acetamide (147 mg, 0.1611 mmol) in MeOH (5 mL) / CH2CI2 (5 mL) and the reaction mixture was allowed to stir at ambient temperature for 2 hours. A further portion of hydrazine hydrate (16.13 mg, 15.68 μL, 0.3222 mmol) was added and the reaction stirred for a further 16 hours. The solvent was removed in vacuo and residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min], The fractions were collected and freeze-dried to give the title compound as a yellow solid (10 mg, 11 %); 1H NMR (400.0 MHz, DMSO) d 1.18 (d, 6H), 3.50-3.53 (m, I H), 4.1 1 (s, 1.4H), 4.33 (s, 0.6H), 7.81 (s, 2H), 7.91 (d, 2H), 8.53 (d, 2H), 9.07 (s, I H), 10.99 (s, 0.7H) and 1 1.16 (s, 0.3H) ppm; MS (ES+) 418.2 [00494] The following compounds were all prepared using a method similar to the one described for Compound IA-82 above. Compound IA-219 2-amino-N-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- l ,3,4-oxadiazol-2-yl]-2-methyl-propanamide 1H NMR (400.0 MHz, DMSO) d 1.18 (d, 6H), 1.21 (s, 6H), 3.43 - 3.53 (m, I H), 7.80 (br s, 2H), 7.88 (d, 2H), 8.08 (s, 1H), 8.54 (d, 2H), 9.01 (s, I H), 10.49 (br s, 1H) and 10.62 (s, 1H) ppm; MS (ES+) 446.2 Compound IA-272 2-amino-N-[5-[3-amino-6-(4-isopropylsuIfonylphenyl)pyrazin-2-yl]- l ,3,4-oxadiazol-2-yl]propanamide I H NMR (400.0 MHz, DMSO) d 1.19 (d, 6H), 1.30 (d, 1.8H), 1.39 (d, 1.2H), 3.46-3.53 (m, 1 H), 4.34 (br s, 0.6H), 4.54 (br s, 0.4H), 7.82 (br s, 2H), 7.91 (d, 2H), 8.50-8.55 (m, 2H), 9.09 (s, I H), 1 1.06 (br s, 0.6H) and 11.17 (br s, 0.4H) ppm; MS (ES+) 432.2
Example 54A: 5-r3-amino-6-(4-isopropylsulfonvlphenyl)pyrazin-2-yH-N-(3-piperidyl> 1,3,4-oxadiazol-2 -amine (Compound IA-199)
SCHEME
IV-AAA
Figure imgf000302_0001
Compound IA-199
[00495] Compound IA-199 was prepared using Method IV-C, Steps 1-2, followed by Method IV-K, Step 1, followed by Method IV-AAA, Step 1.
METHOD IV-AAA
Step 1: 5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-N-(3-piperidyl)-l ,3,4- oxadiazol-2-amine
[00496] DIPEA (173.6 μL, 1.0 mmol), tert-buty\ 3-aminopiperidine-1-carboxylate (99.7 mg, 0.50 mmol) and bromo(tripyrrolidin-1-yl)phosphonium hexafluorophosphate (340.6 mg, 0.73 mmol) were added to a mixture of 5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2- yl]-3H-1,3,4-oxadiazol-2-one (120 mg, 0.33 mmol) in DMF (600 μL) and DMSO (600 μL). The resulting mixture was stirred at room temperature for 4.5 h. The reaction mixture was diluted with EtOAc (5 mL) and saturated aqueous sodium hydrogen carbonate solution (5 mL). The aqueous layer was washed with EtOAc (3 x 5 mL) and the combined organic extracts dried over MgSO4, filtered and concetrated in vacuo. The residue was taken up in methanol (1.2 mL) and HCl (332 μL, 1.0 mmol, 3M solution in methanol) and the resulting solution stirred at room temperature overnight. The reaction mixture was evaporated to dryness and the solid triturated with acetonitrile and then purified further by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were passed through a bicarbonate cartridge and lyopholised to give the title compound as a yellow solid (29.6 mg, 20%); H NMR (400.0 MHz, DMSO) d 1.19 (d, 6H), 1.43-1.48 (m, 2H), 1.64-1.67 (m, I H), 1.99-2.01 (m, 1H), 2.40-2.46 (m, 2H), 2.77-2.80 (m, I H), 3.10-3.14 (m, I H), 3.46-3.55 (m, 2H), 7.80 (br s, 1H), 7.95 (d, 2H), 8.23 (t, 2H), 8.27 (s, I H) and 8.89 (s, 1H) ppm; MS (ES+) 444.25
[00497] The following compounds were all prepared using a method similar to the one described for Compound IA-199 above.
Compound IA-97 N-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-l ,3,4- oxadiazol-2-yl]propane- 1 ,3-diamine 1 H NMR (400.0 MHz, DMSO) d 1.18 (d, 7H), 1.67 (t, 2H), 2.64 (t, 2H), 3.00-3.01 (m, 2H), 3.46-3.50 (m, 1H), 6.75 (br s, 1H), 7.80 (br s, I H), 7.95 (d, 2H), 8.26 (d, 2H) and 8.89 (s, 1H) ppm; MS (ES+) 418.21
Compound IA-109 3-[5-(4-amino-1-piperidyl)-l ,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.19 (d, 6H), 1.45-1.60 (m, 2H), 1.94 (s, 2H), 2.08 (s, 1H), 3.17-3.25 (m, 4H), 3.45 (t, 2H), 4.00 (s, 2H), 7.75 (br s, 1H), 7.93 (d, 2H), 8.32 (d, 2H) and 8.93 (s, 1H) ppm; MS (ES+) 444.21
Compound IA-111 3-[5-(3-aminoazetidin-1-yl)-l ,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylpheny l)pyrazin-2-amine 1 H NMR (400.0 MHz, DMSO) d 1.18 (dd, 6H), 2.35 (br s, 2H), 2.95 (br s, 2H), 3.40-3.55 (m, 1H), 3.85-4.01 (m, 2H), 4.00-4.30 (m, 2H), 7.75 (br s, 1H), 7.94 (d, 2H), 8.27 (d, 2H) and 8.91 (s, 1H) ppm; MS (ES+) 416.2 Compound IA-138 3-[5-[4-(aminomethyl)-1-piperidyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1 H NMR (400.0 MHz, DMSO) d 1.16-1.19 (m, 6H), 1.20 - 1.40 (m, 3H), 1.50-1.95 (m, 3H), 2.08 (s, 1 H), 2.54 (s, 1 H), 2.85-3.35 (m, 3H), 3.40-3.50 (m, I H), 3.95-4.10 (m, 2H), 7.75 (br s, 1H), 7.94 (d, 2H), 8.31 (d, 2H) and 8.92 (s, 1H) ppm; MS (ES+) 458.21
Compound IA-188 3-[5-(3-aminopyrrolidin-1-yl)-l ,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.05 (d, 6H), 1.95-2.05 (m, 1H), 2.20-2.30 (m, I H), 2.95 (t, 3H), 3.40-3.55 (m, 2H), 3.60-3.70 (m, 1H), 3.70-3.80 (m, 2H), 3.80-3.90 (m, I H), 5.15 (s, I H), 7.80 (d, 2H), 8.16 (d, 2H) and 8.78 (s, 1H) ppm; MS (ES+) 430.27
Compound IA-227 3-[5-[3-(aminomethyl)-1-piperidyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine MS (ES+) 458.23
Compound IA-206 3-[5-(3-amino-1-piperidyl)-l ,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 1.19 (d, 6H), 1.45-1.65 (m, 1H), 1.75-1.90 (m, 2H), 2.08 (s, 1H), 2.75-3.20 (m, 5H), 3.40-3.50 (m, 1H), 3.75-3.95 (m, 2H), 7.75 (s, 2H), 7.94 (d, 2H), 8.32 (d, 2H) and 8.91 (s, 1H) ppm; MS (ES+) 444.21
Compound IA-239 5-(4-isopropylsulfonylphenyl)-3-(5-piperazin-1-yl-l ,3,4-oxadiazol-2- yl)pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 1.18 (d, 6H), 2.67 (s, 1H), 2.98 (s, 3H), 3.10 (d, 2H), 3.40-3.50 (m, I H), 3.57-3.60 (m, 4H), 7.75 (br s, 1H), 7.93 (d, 2H), 8.32 (d, 2H) and 8.93 (s, 1H) ppm; MS (ES+) 430.23
Compound IA-318 5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-N-(4-piperidyl)- 1,3,4-oxadiazol-2-amine 1H NMR (400.0 MHz, DMSO) d 1.18 (d, 6H), 1.35-1.45 (m, 2H), 1.95-2.00 (m, 2H), 2.95-3.00 (m, 2H), 3.40-3.55 (m, 2H), 7.95 (d, 2H), 8.25 - 8.35 (m, 3H) and 8.90 (s, 1H) ppm; MS (ES+) 444.2 Example 55: 5-r3-amino-6-(4-isopropvlsulfonvlphenvπpyrazin-2-vll-N-pvrrolidin-3-vl- 1,3,4-oxadiazole-2-carboxamide (Compound IA-114)
SCHEME
Figure imgf000305_0001
Compound IA-114
[00498] Compound IA-1 14 was prepared using Method IV-C, Steps 1-2, followed by
Method IV-AAB, Steps 1-3.
METHOD IV-AAB
Step 1: ethyl 2-(2-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazine-2- carbonyl)hydrazinyl)-2-oxoethanoate
[00499] 3-amino-6-(4-isopropylsulfonylphenyl)pyrazine-2-carbohydrazide (2 g, 5.963 mmol) and Et3N (1.81O g, 2.493 mL, 17.89 mmol) were dissolved in THF (128.0 mL) and treated dropwise with ethyl 2-chIoro-2-oxo-acetate (814.2 mg, 666.3 μL, 5.963 mmol) at O°C. The reaction mixture was warmed slowly to room temperature and stirred for 1.5 h. The reaction mixture was filtered and grey solid washed with THF. The filtrate was evaporated to dryness azeotroping with acetonitrile. Then residue was then triturated with acetonitrile to give the product as a yellow solid (1.52g, 58%); 1H NMR (400 MHZ, DMSO) d 1.19 (m, 6H), 1.32 (m, 3H), 3.34 (m, 1H), 4.32 (m, 2H), 7.88 (m, 2H), 8.56 (m, 2H), 9.07 (s, 1 H), 10.95 (s, 1 H) and 1 1.05 (s, 1 H) ppm; MS (ES+) 436.32 Step 2: ethyl 5-(3-amino-6-(4-(isopropylsuIfonyl)phenyl)pyrazin-2-yl)-l ,3,4-oxadiazoIe-2- carboxylate
[00500] To a stirred solution of ethyl 2-(2-(3-amino-6-(4-(isopropylsulfonyl)phenyl) pyrazine-2-carbonyl)hydrazinyl)-2-oxoacetate (1.1894 g, 2.731 rηmol) was in CH2Cl2 (23.78 mL) was added triethylamine (552.7 mg, 761.3 μL, 5.462 mmol), followed by 4- methylbenzenesulfonyl chloride (520.7 mg, 2.731 mmol) and the resulting solution stirred at room temperature for 3 h. The reaction mixture is diluted with CH2Cl2 and washed with water (1 x 20 mL), saturated aqueous sodium hydrogen carbonate solution (1 x 20 mL) and brine (1 x 20 mL). The organic extracts were dried over MgSO4, filtered and concentrated in vacuo. The residue was triturated with acetonitrile to give the product as a yellow solid (1.03 g, 90%); 1H NMR (400 MHz, DMSO) d 1.37 (m, 6H), 1.54 (m, 3H), 3.25 (m, 1H), 4.64 (m, 2H), 8.00 (m, 2H), 8.20 (m, 2H) and 8.83 (s, 1H) ppm; MS (ES+) 418.19
Step 3: 5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-N-pyrrolidin-3-yl-1,3,4- oxadiazole-2-carboxamide
[00501] To a suspension of ethyl 5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- 1,3,4-oxadiazole-2-carboxylate (100 mg, 0.34 mmol) in ethanol (2 mL), tert-buty\ 3- aminopyrrolidine-1-carboxylate (49.1 mg, 0.26 mmol) was added and the resulting mixture heated under reflux overnight. The reaction mixture was cooled to room temperature and evaporated to dryness. The residue was taken up in CH2Cl2 (2.0 mL) and TFA (400 μL) was added and the reaction mixture stirred overnight at room temperature. The reaction mixture was passed through a bicarbonate cartridge and the filtrate concentrated in vacuo. The residue was passed through a TsOH cartridge eluting the product with 2M Ammonia in methanol (5 mL). The solid was triturated form acetonitrile to give the prodcut as a yellow solid (44.94 mg, 41%); 1H NMR (400.0 MHz, DMSO) d 1.19 (d, 6H), 1.75 (s, I H), 2.00 (d, 1H), 2.73-2.78 (m, 2H), 2.94 (s, I H), 2.95 (dd, 1H), 3.47 (t, 1H), 4.40 (br s, I H), 7.85 (br s, 2H), 7.98 (d, 2H), 8.32-8.34 (m, 2H), 9.09 (s, 1H) and 9.46 (d, 1H) ppm; MS (ES+) 458.22 [00502] The following compounds were all prepared using a method similar to the one described for Compound IA-1 14 above. Compound IA-79 [5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazol- 2-yl]-(1,4-diazepan-1-yl)methanone; MS (ES+) 472.3
Compound IA-81 N-(2-aminocyclohexyl)-5-[3-amino-6-(4- isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazole-2-carboxamide 1H NMR (400.0 MHz, DMSO) d 1.19 (d, 6H), 1.21-1.30 (br s, 1H), 1.40-1.50 (m, I H), 1.65-1.75 (m, 2H), 1.80-1.95 (m, 2H), 2.75 (br s, 2H), 3.45-3.50 (m, 3H), 3.65 (br s, I H), 7.95 (s, 2H), 8.45 (s, 2H), 9.10 (s, 1H) and 9.30 (br s, 1H) ppm; MS (ES+) 486.35
Compound IA-98 [5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazol- 2-yl]-(3-amino-1-piperidyl)methanone I H NMR (400.0 MHz, DMSO) d 1.19 (d, 6H), 1.34- 1.38 (m, I H), 1.48-1.51 (m, I H), 1.76-1.92 (m, 3H), 2.67-2.81 (m, 2H), 3.17-3.29 (m, 2H), 3.50-3.99 (m, I H), 4.09-4.10 (m, 0.5H), 4.12-4.23 (m, 0.5H), 4.24-4.30 (m, 1H), 7.85 (br s, I H), 7.98 (d, 2H), 8.32 (dd, 2H) and 9.09 (s, 1H) ppm; MS (ES+) 472.28
Compound IA-113 butyl 5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- 1,3,4- oxadiazole-2-carboxylate 1 H NMR (400.0 MHz, DMSO) d 0.97 (t, 3H), 1.19 (d, 6H), 1.46 (d, 2H), 1.75-1.78 (m, 2H), 2.08 (s, 1H), 3.47-3.55 (m, 1H), 4.46 (t, 2H), 7.75 (br s, I H), 7.99 (d, 2H), 8.31 (d, 2H) and 9.10 (s, 1H) ppm; MS (ES+) 446.22
Compound IA-120 (3-aminoazetidin-1-yl)-[5-[3-amino-6-(4-isopropylsulfonylphenyl) pyrazin-2-yl]-1,3,4-oxadiazol-2-yl]methanone I H NMR (400.0 MHz, DMSO) d 1.18-1.20 (m, 6H), 2.50 (br s, 1H), 2.55 (s, 1H), 3.05 (br s, 2H), 3.45-3.52 (m, I H), 3.76-3.80 (m, I H), 3.82-3.87 (m, 1H), 4.22-4.26 (m, 1H), 4.31-4.36 (m, 1H), 4.76-4.79 (m, I H), 7.98 (d, 2H), 8.32 (d, 2H) and 9.09 (s, I H) ppm; MS (ES+) 444.28
Compound IA-133 [5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- 1,3,4- oxadiazol-2-yl]-(3-aminopyrrolidin- 1 -yl)methanone 1 H NMR (400.0 MHz, DMSO) d 1.19 (d, 6H), 1.65-1.80 (m, I H), 1.95-2.10 (m, I H), 3.45-3.50 (m, 2H), 3.55-3.75 (m, 3H), 3.95- 4.10 (m, 1 H), 7.75 (br s, 1 H), 7.98 (d, 2H), 8.32 (d, 2H) and 9.09 (s, 1 H) ppm; MS (ES+) 458.37 Compound IA-255 5-[3-amino-6-(4-isopropylsuIfonylphenyl)pyrazin-2-yl]-N-(3- piperidylmethyl)-1,3,4-oxadiazole-2-carboxamide I H NMR (400.0 MHz, DMSO) d 1.05- 1.10 (m, I H), 1.19 (d, 6H), 1.25-1.35 (m, 1H), 1.55-1.59 (m, I H), 1.73-1.75 (m, I H), 2.19- 2.22 (m, I H), 2.33-2.40 (m, 1H), 2.80-2.82 (m, I H), 2.91-2.94 (m, 1H), 3.18 (s, I H), 3.18- 3.21 (m, 2H), 3.47-3.50 (m, I H), 7.85 (br s, I H), 7.98 (d, 2H), 8.33 (d, 2H), 9.09 (s, 1H) and 9.44 - 9.47 (m, I H) ppm; MS (ES+) 486.29
Example 56A: (-2S)-N-r5-f3-amino-6-(4-isoDropvlsulfonvlphenvl')pvrazin-2-vll-l .3.4- oxadiazol-2-yl]piperidine-2-carboxamide (Compound IA-211)
SCHEME
Method IV-C SlepS 1-2
Figure imgf000308_0001
Compound IA*211
[00503] Compound IA-21 1 was prepared using Method IV-C, Steps 1-2, followed by
Method IV-AY, Steps 1-2, followed by Method IV-AAC, Step 1.
METHOD IV-AAC
Step 1: (2S)-N-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazol-2- yl]piperidine-2-carboxamide
[00504] Pd on C, wet, degussa (50 mg, ) was added to a stirred solution of (S)-benzyl 2-
(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)-l ,3,4-oxadiazol-2-ylcarbamoyl) piperidine-1-carboxylate (251 mg, 0.25 mmol) in MeOH (5 mL) / EtOAc (5 mL) and the reaction mixture was placed under an atmosphere of H2 . The reaction was stirred at ambient temperature for 17 hours. Once the reaction had gone to completion, the Pd was removed by filtration and the solvent was removed in vacuo. The material was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH3CN) over 16 minutes at 25 mL/min]. The fractions were collected and freeze-dried to give the product as a yellow solid (56.4 mg, 39%); 1 H NMR (400.0 MHz, DMSO) d 1.19 (d, 6H), 1.38-1.49 (m, 2H), 1.51-1.61 (m, 1H), 1.80-1.83 (m, 1H), 1.89-1.92 (m, 1H), 2.09-2.1 1 (m, I H), 3.01 (br s, 2H), 3.19-3.23 (m, 1H), 3.47-3.51 (m, I H), 4.13 (d, 1H), 4.31 (br s, 1H), 7.81 (s, 2H), 7.91 (d, 2H), 8.52 (d, 2H), 9.06 (s, 1 H) and 1 1.04 (br s, 1 H) ppm; MS (ES+) 472.3
[00505] The following compounds were all prepared using a method similar to the one described for Compound IA-211 above.
Compound IA-160 (lR,4S,6S)-N-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- l ^-oxadiazol^-yll-S-azabicycloβ^.lJheptane-ό-carboxarnide 1H NMR (400.0 MHz, DMSO) d 1.18 (d, 6H), 1.34-1.45 (m, 2H), 1.55-1.76 (m, 4H), 2.68 (br d, 1H), 3.44-3.52 (m, 1H), 3.64 (s, 0.6H), 3.76 (s, 0.4H), 4.25 (s, 0.6H), 4.33 (s, 0.4H), 7.88 (d, 1.2H), 7.90 (br s, 2H), 7.92 (d, 0.8H), 8.31 (d, 0.8H), 8.53 (d, 1.2H), 9.00 (d, 1H), 10.43 (s, 0.4H) and 10.86 (s, 0.6H) ppm; MS (ES+) 484.3
Compound IA-217 (2S)-N-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- 1 ,3,4- oxadiazol-2-yl]pyrrolidine-2-carboxamide 1H NMR (400.0 MHz, DMSO) d 1.18 (d, 6H), 1.67-1.72 (m, I H), 2.04-2.21 (m, 2H), 3.00 (br s, 2H), 3.35-3.41 (m, I H), 3.45-3.59 (m, 3H), 4.28-4.34 (m, 1H), 7.82 (br s, 2H), 7.88-7.92 (m, 2H), 8.44-8.53 (m, 2H), 9.00 - 9.01 (2 x s, 1H) and 10.87 (s, 1H) ppm; MS (ES+) 458.3
Example 57A: 5-(4-isopropvlsulfonvlphenvlV3-r3-r4-(methvlaminomethvOphenvπisoxazol- 5-yl]pyrazin-2-amine (Compound IIA-7)
SCHEME
-AAD
Figure imgf000310_0001
Compound IIA-7
[00506] Compound IIA-7 was prepared using Method IV-F, Steps 1-2, followed by Method IV-AAD, Steps 1-4.
METHOD IV-AAD
Step 1: ført-butyl N-(3-ethynyl-5-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)N-tert- butoxycarbonyl-carbamate
[00507] tert-butyl N-[5-bromo-3-(2-trimethylsilylethynyl)pyrazin-2-yl]-N-tert- butoxycarbonyl-carbamate (3 g, 6.377 mmol) and (4-isopropylsulfonylphenyl)boronic acid (1.491 g, 6.536 mmol) were dissolved in MeCN (60.00 mL) then treated with water (12.00 mL) and K3PO4 (2.706 g, 12.75 mmol) then degassed/flushed nitrogen (x5 cycles). Treated with Pd[P(tBu)3]2 (162.9 mg, 0.3188 mmol) and reflushed Vac/Nitrogen x5. The resulting mixture was stirred at room temperature for 1 h. The reaction mixture was poured quickly into a mixture of ethyl acetate (500 mL), water (90 mL) and 1% aqueous sodium metabisulphite at 4 °C, shaken well and the layer separated. The organic fraction was dried over MgSC>4, filtered and the filtrate was treated with 3-mercaptopropyl ethyl sulphide on silica (0.8mmo!/g)(lg), pre-absorbed onto silica gel then purified by column chromatography on silica gel eluting with 30-40% EtOAc/petroleu ether. Product fractions were combined and concentrated in vacuo to leave the product as a yellow/ brown viscous oil. Triturated with petroleum ether and some diethyl ether and a small amount of dichloromethane added. Left to stand at room temperature for 30 min and beige crystals formed, isolated by filtration to leave the product as a beige solid (1.95 g, 61 %); 1 H NMR (400 MHz, DMSO) d 1.20 (m, 6H), 1.39 (s, 18H), 3.50 (m, 1H), 5.01 (s, 1H), 8.03 (m, 2H), 8.46 (m, 2H) and 9.37 (s, I H) ppm.
Step 2: tert-butyl N-[5-(4-(isopropylsulfonyl)phenyl)-3-(3-(4-methyl)phenylisoxazol-5- yl)pyrazin-2-yl]-N-tert-butoxycarbonyl-carbamate
[00508] To a solution of tert-butyl N-tert-butoxycarbonyl-N-[3-ethynyl-5-(4- isopropylsulfonylphenyl)pyrazin-2-yl]carbamate (6.8 g, 13.56 mmol) and N-hydroxy-4- methyl-benzimidoyl chloride (2.706 g, 13.56 mmol) in THF (141.6 mL) at room temperature was added TEA (1.646 g, 2.267 mL, 16.27 mmol) dropwise over 10 min. The mixture was stirred at room temperature overnight then at 60°C for 2 h. The reaction mixture was concentrated under reduced pressure, dissolved in CH2CI2 (30 mL) and washed with brine (1 x 50 mL) and aqueous NaHCCb (1 x 50 mL). The organic extracts were dried over MgSC>4 then decanted onto a silica gel column (300ml). Elution with 20%EtOAc/petroleum ether to give the product (7.1 g, 82%); 1 H NMR (400 Mhz, DMSO) d 1.21 (m, 6H), 1.33 (s, 18H), 3.34 (s, 3H), 3.55 (m, 1H), 7.39 (m, 2H), 7.92 (m, 2H), 8.01 (s, 1H), 8.07 (m, 2H), 8.66 (m, 2HO and 9.51 (s, 1H) ppm
Step 3: tert-butyl N-[5-(4-(isopropylsulfonyl)phenyl)-3-(3-(4-bromomethyl)phenylisoxazol- 5-yl)pyrazin-2-yl]-N-tert-butoxycarbonyl-carbamate [00509] tert-butyl N-tert-butoxycarbonyl-N-[5-(4-isopropylsulfonylphenyl)-3-[3-(p- tolyl)isoxazol-5-yl]pyrazin-2-yl]carbamate (1 g, 1.575 mmol) was dissolved in ethylacetate (10 mL) and NBS (364.5 mg, 2.048 mmol) and AIBN (25.86 mg, 0.1575 mmol) were added. The resulting mixture was heated to 75°C and placed under a bright lamp for Ih. After this time, the reaction mixture was concentrated in vacuo to an oil and this was used directly in the next stage without further pufication
Step 4: 5-(4-isopropylsulfonylphenyl)-3-[3-[4-(methylaminomethyl)phenyl]isoxazol-5- yl]pyrazin-2-amine
[00510] tert-butyl N-[3-[3-[4-(bromomethyl)phenyl]isoxazol-5-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-yl]-N-tert-butoxycarbonyl-carbamate (60 mg, 0.08408 mmol) was added to a solution of methylamine in ethanol solution (791.3 mg, 8.408 mmol) in ethanol (3 mL). The reaction mixture was stirred at room temperature for 1 h and then the solvent removed in vacuo to an oil. The oil was redisoolved in CH2Cl2 (10ml) and concentrated to an oil to remove any excess amine. The oil was taken up in CH2CI2 (5 mL) and TFA (479.4 mg, 323.9 μL, 4.204 mmol) added. The mixture was stirred at room temperature for 1 h, and the reaction mixture concentrated in vacuo. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were passed through a bicarbonate cartridge and lyopholised to give the title compound as a yellow solid (13.6 g, 28 % yield); 1H NMR (400 MHz, DMSO) d 1.22 (d, 6H), 2.6-2.65 (m, 3H), 3.5-3.6 (m, 1H), 4.2-4.25 (m, 2H), 7.2- 73 (br s, 2H), 7.65 (d, 2H), 7.82 (s, 1H), 7.85 (d, 2H), 8.1 (d, 2H), 8.4 (d, 2H), 8.85 (br s, 2H) and 8.92 (s, 1H) ppm; MS (ES+) 464.4
[00511] The following compounds were all prepared using a method similar to the one described for Compound IIA-7 above.
Compound IIA-4 2-(2-(4-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2- yl)isoxazol-3-yl)benzylamino)ethoxy)ethanol 1H NMR (400 MHz, DMSO) d 1.22 (d, 6H), 3.2-3.25 (m, 2H), 3.5-3.6 (m, 2H), 3.6-3.63 (m, 2H), 3.5-3.8 (m, 2H), 4.3-4.35 (m, 2H), 4.75 (br s, 1H), 7.2-73 (br s, 2H), 7.65 (d, 2H), 7.82 (s, 1H), 7.95 (d, 2H), 8.1 (d, 2H), 8.4 (d, 2H) and 8.9-9.05 (m, 3H) ppm; MS (ES+) 538.4 Compound IIA-5 3-[3-[4-(aminomethyl)phenyl]isoxazol-5-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.22 (d, 6H), 3.5- 3.6 (m, I H), 4.2-4.25 (m, 2H), 7.2-73 (br s, 2H), 7.65 (d, 2H), 7.82 (s, 1H), 7.95 (d, 2H), 8.1 (d, 2H), 8.4 (d, 2H), 8.2 (br s, 2H) and 8.97 (s, 1H) ppm; MS (ES+) 450.4 Compound IIA-6 5-(4-(isopropylsulfonyl)phenyl)-3-(3-(4-
((propylamino)methyl)phenyl)isoxazol-5-yl)pyrazin-2-amine I H NMR (400 MHz, DMSO) d 0.95 (t, 3H), 1.22 (d, 6H), 1.6-1.7 (m, 2H), 2.9-3.0 (m, 2H), 3.5-3.6 (m, 1H), 4.2-4.25 (m, 2H), 7.2-73 (br s, 2H), 7.65 (d, 2H), 7.82 (s, 1H), 7.95 (d, 2H), 8.1 (d, 2H), 8.4 (d, 2H), 8.8 (br s, 2H) and 8.97 (s, 1H) ppm; MS (ES+) 492.4
Compound IIA-8 3-[3-[4-[(isopropylamino)methyl]phenyl]isoxazol-5-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.2 (d, 6H), 1.3 (d, 6H), 3.5-3.6 (m, I H), 4.2-4.25 (m, 2H), 7.2-73 (br s, 2H), 7.65 (d, 2H), 7.82 (s, 1H), 7.95 (d, 2H), 8.1 (d, 2H), 8.4 (d, 2H), 8.7 (br s, 2H) and 8.95 (s, I H) ppm; MS (ES+) 492.4 Compound IIA-9 2-(4-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)isoxazol-3- yl)benzylamino)ethanol I H NMR (400 MHz, DMSO) d 1.22 (d, 6H), 3.0-3.1 (m, 2H), 3.5- 3.6 (m, 1H), 3.65-3.7 (m, 2H), 4.2-4.25 (m, 2H), 5.3 (br s, 1H), 7.2-73 (br s, 2H), 7.65 (d, 2H), 7.82 (s, 1H), 7.95 (d, 2H), 8.1 (d, 2H), 8.4 (d, 2H), 8.8 (br s, 2H) and 8.87 (s, 1H) ppm; MS (ES+) 494.3
Compound IIA-10 3-[3-[4-(ethylaminomethyl)phenyl]isoxazol-5-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400 MHz, DMSO) d 1.22 (d, 6H), 1.25 (t, 3H), 3.0-3.1 (m, 2H), 3.5-3.6 (m, 1H), 4.2-4.25 (m, 2H), 7.2-73 (br s, 2H), 7.65 (d, 2H), 7.82 (s, I H), 7.95 (d, 2H), 8.1 (d, 2H), 8.4 (d, 2H), 8.8 (br s, 2H) and 8.97 (s, I H) ppm; MS (ES+) 478.4
Compound IIA-11 l-(4-(5-(3-amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)isoxazol- 3-yl)benzylamino)propan-2-ol 1H NMR (400 MHz, DMSO) d 1.05 (d, 3H), 1.22 (d, 6H), 3.0-3.1 (m, 2H), 2.65-2.7 (m, 1H), 2.8-2.85 (m, 1H), 3.5-3.6 (m, I H), 3.8-3.85 (m, 1H), 4.2- 4.25 (m, 2H), 5.3-5.33 (m, 1H), 7.2 (br s, 2H), 7.65 (d, 2H), 7.82 (s, 1H), 7.85 (d, 2H), 8.02 (d, 2H), 8.35 (d, 2H), 8.8 (br s, 2H) and 8.87 (s, 1H) ppm; MS (ES+) 508.4 Example 58A: 5-(4-isoDropvlsulfonylphenvl)-3-r5-r4-ri-(methvlaminokthvllphenvπ- 1,3,4- oxadiazol-2-yl]pyrazin-2-amine (Compound IA-212)
SCHEME
Figure imgf000314_0001
Compound IA-212
[00512] Compound IA-212 was prepared using Method IV-C, Steps 1-2, followed by Method IV-R, Step 1 , followed by Method IV-AAE, Step 1.
METHOD IV-AAE
Step 1: 5-(4-isopropylsulfonylphenyl)-3-[5-[4-[l-(methylamino)ethyl] phenyl]- 1 ,3,4- oxadiazol-2-yl]pyrazin-2-amine
[00513] A mixture of l-[4-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]- 1 ,3,4- oxadiazol-2-yl]phenyl]ethanone (130 mg, 0.2805 mmol), methylamine hydrochloride (37.88 mg, 0.5610 mmol), Ti(OiPr)4 (159.4 mg, 165.5 μL, 0.5610 mmol) and triethylamine (56.77 mg, 78.20 μL, 0.5610 mmol) was stirred at room temperature in ethanol (2 mL) under nitrogen overnight. The reaction mixture was treated with sodium borohydride (15.92 mg, 16.85 μL, 0.4208 mmol) and stirred at room temperature over weekend and then was quenched with aqueous ammonia (ImL cone in 4mL water). The mixture was extracted with dichloromethane and the organic extracts dried over MgSO4 and concentrated in vacuo. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were passed through a bicarbonate cartridge and concentrated in vacuo. The solid was triturated with acetonitrile to give the product as a pale yellow solid (27.0 mg, 22%); 1 H NMR (400 MHz, DMSO) d 1.20 (d, 6H), 1.28 (d, 3H), 4.48 (m, 1H), 3.69 (q, 1H), 7.72 (d, 2H), 7.97 (d, 2H), 7.98 (v br s, 2H), 8.12 (d, 2H) and 9.07 (s, 1H) ppm; MS (ES+) 479.3
Example 59A: 5-(4-isopropylsulfonylphenyl)-3-[5-[2-methyl-4-(methylaminomethyl) phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin:2-amine (Compound IA-166)
SCHEME
Figure imgf000315_0001
Compound IA-166
Compound IA-166 was prepared using Method IV-B, Steps 1-4, followed by Method
IV-AAF, Step 1.
METHOD IV-AAF
Step 1: 5-(4-isopropylsulfonylphenyl)-3-[5-[2-methyl-4-(methylaminomethyl)phenyl]-l ,3,4- oxadiazol-2-yl]pyrazin-2-amine
[00514] To a solution of tert-butyl N-[[4-[5-[3-amino-6-(4-isopropylsulfonylphenyl) pyrazin-2-yl]-l ,3,4-oxadiazol-2-yl]-3-methyl-phenyl]methyl]-N-methyl-carbamate (120 mg, 0.2074 mmol) in CH2Cl2 (10 mL) was added TFA (709.5 mg, 479.4 μL, 6.222 mmol) and the resulting solution stirred at room temperature for 1 h. The reaction mixture was concentrated in vacuo and redissolved in CH2Cl2 (20ml) and concentrated. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. Product fractions were combined and lyopholised to give the product as a yellow solid (48.0 mg, 39 %); 1 H NMR (400 MHz, DMSO) d 1.2 (d, 6H), 2.6 (s, 3H), 2.75 (s, 3H), 3.4-3.5 (m, 1H), 4.25 (s, 2H), 7.7 (d, 1H), 7.72 (s, 1H), 8.0-8.1 (m, 3H), 8.2 (d, I H), 8.4 (d, 2H), 8.8 (br s, 2H) and 9.2 (s, 1H) ppm; MS (ES+) 479.4 Example 6OA: 5-r3-amino-6-(4-isopropvlsulfonylphenyl)pvrazin-2-yll-l .3.4-oxadiazole-2- carboxylic acid (Compound IA-128)
SCHEME
Figure imgf000316_0001
Compound IA-128
[00515] Compound IA-128 was prepared using Method IV-C, Steps 1 -2, followed by Method IV-AAB, Steps 1-2, followed by Method IV-AAG, Step 1.
METHOD IV-AAG
Step 1: 5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazole-2- carboxylic acid
[00516] A solution of ethyl 5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-y I]- 1,3,4- oxadiazole-2-carboxylate (50 mg, 0.1 198 mmol) in NaOH (59.90 μL of 1 M, 0.05990 mmol) was stirred at room temperature for 1 h. Water (0.5 mL) was added and the reaction mixture stirred at room temperature for 5 min and then filtered. The yellow solid obtained was dried under vacuum to give the product (30.93 mg, 62%); 1 H NMR (400 MHz, DMSO) d 1.19 (d, 6H), 3.40-3.49 (m, 1H), 7.90 (br s, 2H), 7.96 (d, 2H), 8.32 (d, 2H) and 9.00 (s, 1H) ppm; MS (ES+) 390.13
Example 61 A: 3-r5-ethvnvl-1.3.4-oxadiazol-2-vlV5-(4-isopropylsulfonylphenyl)pvrazin-2- amine (Compound IA-258)
SCHEME
Figure imgf000317_0001
Compound IA-258
Compound IA-258 was prepared using Method IV-C, Steps 1-2, followed by Method IV- AAH, Step 1.
METHOD IV-AAH
Step 1: 3-(5-ethynyl-1,3,4-oxadiazol-2-yI)-5-(4-isopropylsulfonylphenyl)pyrazin-2-amine
[00517] Dibromo(triphenyl)phosphorane (1.208 g, 2.862 mmol) was added to a suspension of 3-trimethylsilylprop-2-ynoic acid (84.8 mg, 0.60 mmol) and 3-amino-6-(4- (isopropylsulfonyl)phenyl)pyrazine-2-carbohydrazide (200 mg, 0.60 mmol) in acetonitrile (3.000 mL) at room temperature and the resulting solution stirred for 30 min. DIPEA (385.4 mg, 519.4 μL, 2.982 mmol) was then added and a precipitate quickly formed. The resulting mixture was stirred at room temperature for Ih and was then filtered. The reaction mixture was concentrated in vacuo and the residue taken up in methanol (5 mL) and potassium carbonate (131.9 mg, 0.9541 mmol) added and the resulting solution stirred at room temperature overnight. The reaction mixture was diluted with ethyl acetate (5 mL) and water (5 mL) and the layers separated. The aqueous layer was extracted further with ethyl acetate (2 x 5 mL), dried over MgSO4 and concentrated in vacuo. The material was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. Product fractions were freeze dried to leave the product as a yellow solid (56.1 mg, 27 % yield); 1 H NMR (400 MHz, DMSO) d 1.18 (m, 6H), 3.44 (m, I H), 5.48 (s, I H), 7.96 (m, 2H), 8.32 (m, 2H) and 9.08 (s, 1 H) ppm; MS (ES+) 370.14
Example 62A: 2-r5-r3-amino-6-(4-isopropvlsulfonvlphenvπpvrazin-2-vl1-1.3.4-oxadiazol-2- yljacetic acid (Compound IA-78)
SCHEME
Figure imgf000318_0001
Compound IA-78
[00518] Compound IA-78 was prepared using Method IV-C, Steps 1-2, followed by Method IV-X, Steps 1-2, followed by Method IV-AAI, Step 1.
METHOD IV-AAI
Step 1: 2-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazol-2- yl]acetic acid
[00519] TFA (500 μL, 6.490 mmol) was added to a stirred solution of tert-butyl 2-(5-(3- amino-6-(4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)-l ,3,4-oxadiazol-2-yl)acetate (45 mg, 0.083 mmol) in CH2CI2 (5 mL) and the reaction stirred at ambient temperature for 18 h. The solvent was removed in vacuo and the residue azeotroped with CH2CI2 (2 x 5 mL) and ether (2 x 5 mL). The material was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The fractions were collected and freeze-dried to give the title compound as a yellow solid (16.7 mg, 49 %); 1H NMR (400 MHz, DMSO) d 1.19 (d, 6H), 3.45 (m, 1H), 4.27 (s, 2H), 7.85 (br s, 2H), 7.96 (d, 2H), 8.30 (d, 2H), 9.04 (s, 1H) and 13.30 (s, 1H) ppm; MS (ES+) 404.2
Example 63A: 5-(4-isopropvlsulfonvlphenvπ-3-f5-f2-methoxv-4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine (Compound IA-171)
SCHEME
Figure imgf000319_0001
Compound IA-171
[00520] Compound IA-171 was prepared using Method IV-B, Steps 1-4, followed by Method IV-AAJ, Steps 1 -3.
METHOD IV-AAJ
Step 1: di-?err-butyl(5-(4-(isopropylsulfonyl)phenyl)-3-(5-(2-methoxy-4-methylphenyl)- 1,3,4-oxadiazol-2-yl)pyrazin-2-yl)iminodicarbonate
[00521] Di-tert-butyldicarbonate (703.2 mg, 740.2 μL, 3.222 mmol) and DMAP (7.872 mg, 0.06444 mmol) were added to a suspension of 5-(4-isopropylsulfonylphenyl)-3-[5-(2- methoxy-4-methyl-phenyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine (300 mg, 0.6444 mmol) in a mixture of acetonitrile (10 mL) and THF (10 mL). The reaction mixture was stirred at room temperature for 2h and then heated at 50°C for 2 h. The reaction mixture was cooled to room temperature and concentrated in vacuo. The residue was purified by column chromatography on silica eluting with 20% diethyl ether/ petroleum ether. Product fractions were combined and concentrated in vacuo to leave the product (253 mg, 59%); MS (ES+) 666.31
Step 2: Di-tert-butyl(5-(4-(isopropylsulfonyl)phenyl)-3-(5-(2-methoxy-4- methylaminomethylphenyl)-1,3,4-oxadiazol-2-yl)pyrazin-2-yl)iminodicarbonate
[00522] NBS (120.3 mg, 0.6760 mmol) and AIBN (17.08 mg, 0.1040 mmol) were added to a solution of tert-butyl N-tert-butoxycarbonyl-N-[5-(4-isopropylsulfonylphenyl)-3-[5-(2- methoxy-4-methyl-phenyl)-l ,3,4-oxadiazol-2-yl]pyrazin-2-yl]carbamate (346.2 mg, 0.5200 mmol) in ethylacetate (40 mL). The resulting mixture was heated to reflux for 2 h while under a bright lamp. The reaction mixture was cooled to room temperature and added directly to methylamine in ethanol (2.447 g, 26.00 mmol) at room temperature. The reaction mixture was stirred at room temperature for 30 min and then concentrated in vacuo to leave an oil. The oil was redissolved in CH2CI2 (50ml) and concentrated in vacuo to remove any excess amine. The product was purified by column chromatography on silica eluting with 5 % MeOH/ CH2CI2. Product fractions were combined and concentrated in vacuo to leave the product as a yellow oil. (148 mg, 41%)
Step 3: 5-(4-isopropylsulfonylphenyl)-3-[5-[2-methoxy-4-(methylaminomethyl)phenyl]- l ,3,4-oxadiazol-2-yl]pyrazin-2-amine
[00523] TFA (393.7 mg, 266.0 μL, 3.453 mmol) was added to a solution of tert-butyl N- tert-butoxycarbonyl-N-[5-(4-isopropylsulfonylphenyl)-3-[5-[2-methoxy-4- (methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-yl]carbamate (80 mg, 0.1 151 mmol) CH2Cl2 (10 mL). The resulting mixture was stirred at room temperature for 1 h, and then concentrated in vacuo to leave an oil. The oil was re-dissolved in CH2CI2 (10ml), and evaporated to dryness. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The fractions were collected and freeze-dried to give the title compound as a yellow solid (27.1 mg, 39%); 1H NMR (400 MHz, DMSO) d 1.3 (d, 6H), 2.65-2.7 (m, 3H), 3.4-3.5 (m, 1H), 4.0 (s, 3H), 4.25-4.3 (m, 2H), 7.25 (d, 1H), 7.5 (s, 1H), 8.0 (d, 2H), 8.1 (d, 1H), 8.38 (d, 2H), 8.92 (br s, 2H) and 9.1 (s, 1H) ppm; MS (ES+) 495.3
Example 64A: 5-(2-fluoro-4-isopropvlsulfonvl-phenvlV3-[5-r4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine (Compound IA-292)
SCHEME AAK
Figure imgf000321_0001
Compound IA-292
[00524] Compound IA-292 was prepared using Method IV-AAK, Steps 1-5.
METHOD IV-AAK
Step 1: tert-buty\ 4-(2-(3-amino-6-bromopyrazine-2-carbonyl)hydrazinecarbonyI) benzy l(methy l)carbamate
[00525] To a solution of tert-butyl N-[[4-(hydrazinecarbonyl)phenyl]methyl]-N-methyl- carbamate (1 g, 3.580 mmol) in DMF (7.769 mL) and 2-amino-5-bromo-pyridine-3- carboxylic acid (776.9 mg, 3.580 mmol) was added triethylamine (724.5 mg, 997.9 μL, 7.160 mmol) followed by TBTU (1.724 g, 5.370 mmol). The resulting mixture was stirred at room temperature for 48 h. The reaction mixture was diluted with ethyl acetate (20 mL) and water (20 mL) and the layers separated. The aqueous layer was extracted further with ethyl acetate (2 x 20 mL) and combined organic extracts washed with saturated aqueous sodium hydrogen carbonate s olution (1 x 20 mL), brine (1 x 20 mL), dried over MgSCU and concentrated in vacuo. The residue was triturated with CH2CI2 to give the product as a white solid (1.71 g, 58% yield); 1H NMR (400.0 MHz, DMSO) d 1.39-1.45 (m, 9H), 2.80 (s, 3H), 4.45 (s, 2H),
4.45 (s, 2H), 7.28 (s, 2H), 7.35 (d, 2H), 7.90 (d, 2H), 8.20 (d, 2H), 8.24 (d, 1H), 10.50 (s, 1H) and 10.54 (s, 1H) ppm; MS (ES+) 480.16
Step 2: tert-butyl 4-((5-(3-amino-6-bromopyrazin-2-yl)-1,3,4-oxadiazol-2-yl)methyl) benzy l(methy l)carbamate
[00526] To a solution of tert-butyl N-[[4-[[(2-amino-5-bromo-pyridine-3- carbonyOaminoJcarbamoyllphenylJmethylJ-N-methyl-carbamate (992.3 mg, 2.074 mmol) in dry MeCN (14.88 mL) at 0°C was added DIPEA (804.2 mg, 1.084 mL, 6.222 mmol) followed by dibromo(triphenyl)phosphorane (1.185 g, 2.696 mmol) portionwise and the resulting mixture stirred at 0 °C for 1 h and then at room temperature overnight. The reaction mixture was evaporated to dryness and then purified by column chromatography using the ISCO column comapnion system (40 g column, 0-20 % EtOAc/ petroleum ether. Product fractions were combined and concentrated in vacuo to leave the product as a white solid (681.6 mg, 63 % yield); 1H NMR (400.0 MHZ, DMSO) d 1.39-1.46 (d, 9H, 4.48 (d, 2H),
7.46 (d, 2H), 8.22 (d, 2H), 8.32 (d, 1H) and 8.49 (d, 1H) ppm; MS (ES+) 462.12
Step 3: tert-butyl N-[[4-[5-[3-[bis(tert-butoxyearbonyl)amino]-6-brorno-pyrazin-2-yl]- 1,3,4- oxadiazol-2-yl]phenyl]methyl]-N-methyI-carbamate
[00527] Di-tert-butyl dicarbonate (1.306 g, 1.375 mL, 5.984 mmol) was added to a stirred solution of tert-butyl N-[[4-[5-(3-amino-6-bromo-pyrazin-2-yl)-1,3,4-oxadiazol-2- yl]phenyl]methyl]-N-methyl-carbamate (885 mg, 1.496 mmol) and DMAP (18.28 mg, 0.1496 mmol) in anhydrous THF (20 mL) at room temperature. The reaction was allowed to stir at room temperature for 18 h. Additional DIPEA (580.0 mg, 781.7 μL, 4.488 mmol) and di-tert-butyl dicarbonate (1.306 g, 1.375 mL, 5.984 mmol) were added and the reaction stirred at room temperature for a further 2 h. CH2CI2 (10 mL) was added to aid solubility and the reaction stirred at room temperature overnight. The solvent was removed in vacuo and the resiude purified by column chromatography (ISCO Companion, 40 g column, elueting with 0 to 50% EtOAc/Petroleum Ether, loaded in CH2CI2) to give the product as an off-white solid (810 mg, 82% yield); 1 H NMR (400.0 MHz, DMSO) d 1.26 (s, 18H), 1.37 - 1.45 (m, 9H), 2.85 (br s, 3H), 4.49 (s, 2H), 7.50 (d, 2H), 8.15 (d, 2H) and 8.95 (d, 2H) ppm
Step 4: tert-butyl 4-(5-(3-bis(tert-butoxycarbonyl)amino-6-(2-fluoro-4- (isopropylsulfonyl)phenyl)pyrazin-2-yl)-1,3,4-oxadiazol-2-yl)benzyl(methyl)carbamate
[00528] tert-butyl N-[[4-[5-[3-[bis(tert-butoxycarbonyl)amino]-6-bromo-pyrazin-2-yl]- l ,3,4-oxadiazol-2-yl]phenyl]methyl]-N-methyl-carbamate (100 mg, 0.1512 mmol) was dissolved in DMF (1 mL) and 2-(2-fluoro-4-isopropylsulfonyl-phenyl)-4,4,5,5-tetramethyl- 1,3,2-dioxaborolane (74.44 mg, 0.2268 mmol) and Pd(PPh3)2Cl2 (6.254 mg, 0.01512 mmol) were added. Na2CO3 (226.8 μL of 2 M, 0.4536 mmol) was added and the reaction heated at 80 °C under an atmosphere of nitrogen for 1 h in a sealed tube. The reaction mixture was partitioned between EtOAc (5 mL) and water (5 mL) and the aqueous layer extracted with EtOAc (2 x 5 mL). The combined organic extracts were washed with water (3 x 5 mL), brine (2 x 5 mL), dried over MgSO4, filtered and concentrated in vacuo. The residue was purified by column chromatography (ISCO Companion, 24 g column, elueting with 0 to 50% EtOAc/Petroleum Ether, loaded in CH2CI2) to give the product as an off-white solid that was used without further purification (1 14.4 mg, 96 % yield)
Step 5: 5-(2-fluoro-4-isopropylsulfonyl-phenyl)-3-[5-[4-(methylaminomethyl)phenyl]- 1,3,4- oxadiazol-2-yI]pyrazin-2-amine
[00529] TFA (1 mL, 12.98 mmol) was added to a solution of tert-butyl 4-(5-(3-bis(tert- butoxycarbonyl)amino-6-(2-fluoro-4-(isopropylsulfonyl)phenyl)pyrazin-2-yl)- 1,3,4- oxadiazol-2-yl)benzyl(methyl)carbamate (1 14 mg, 0.1456 mmol) in CH2Cl2 (5 mL). The reaction was stirred at room temperature for 2 h. The solvent was removed in vacuo and the residue azeotroped with CH2Cl2 (x 2) and ether (x 2). The material was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were collected, passed through a sodium bicarbonate cartridge and freeze- dried to give the title compound as a yellow solid (43.5 mg, 62 % yield); 1 H NMR (400.0 MHz, DMSO) d 1.23 (d, 6H), 2.29 (s, 3H), 3.58 (m, 1H), 3.76 (s, 2H), 7.60 (d, 2H), 7.88 (d, 2H), 8.00 (br s, 2H), 8.10 (d, 2H), 8.32 (t, I H) and 8.80 (s, I H) ppm; MS (ES+) 483.3 [00530] The following compounds were all prepared using a method similar to the one described for Compound IA-292 above. Additionally, compounds Pl to P72, P146 and P149 can also be made using a methodology similar to the one described in Method AAK.
Compound IA-290 5-[4-isopropylsulfonyl-3-(trifluoromethoxy)phenyl]-3-[5-[4- (methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 1.24 (d, 6H), 2.61 (s, 3H), 3.53 (sept, 1H), 4.24 (s, 2H), 7.76 (d, 2H), 8.08 (d, 1H), 8.19 (d, 2H), 8.35 (s, 1H), 8.41 (dd, I H) and 9.17 (s, 1H) ppm; MS (ES+) 549.2
Compound IA-293 5-(4-isopropylsulfonyl-2-methyl-phenyl)-3-[5-[4- (methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.22 (d, 6H), 2.28 (s, 3H), 2.58 (s, 3H), 3.48 (d, 1H), 3.74 (s, 2H), 7.58 (d, 2H), 7.81 - 7.85 (m, 4H), 8.05 (d, J = 8.2 Hz, 2H) and 8.60 (s, I H) ppm; MS (ES+) 479.3
Compound IA-294 5-(4-(cyclopentylsulfonyl)phenyl)-3-(5-(4- ((methylamino)methyl)phenyl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-amine
Compound IA-295 5-[5-amino-6-[5-[4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2- yl]pyrazin-2-yl]-2-isopropylsulfonyl-benzonitrile 1H NMR (400.0 MHz, DMSO) d 1.28 (d, 6H), 2.30 (s, 3H), 3.63 (m, 1H), 3.77 (s, 2H), 7.62 (d, 2H), 8.13 (d, 2H), 8.22 (d, 1H), 8.71 (dd, I H), 8.87 (s, 1H) and 9.19 (s, I H) ppm; MS (ES+) 490.3
Compound IA-298 3-(5-(4-((methylamino)methyl)phenyl)-1,3,4-oxadiazol-2-yl)-5-(4-(l- methylpyrrolidin-3-ylsulfonyl)phenyl)pyrazin-2-amine
Compound IA-300 5-(5-isopropylsulfonyl-2-pyridyl)-3-[5-[4-(methylaminomethyl)phenyl]- 1,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.23 (d, 6H), 2.64 (s, 3H), 3.59 (m, 1H), 4.29 (s, 2H), 7.77 (d, 2H), 8.28 (d, 2H), 8.37 - 8.39 (m, 1H), 8.56 (d, I H), 8.87 (br s, 2H), 9.05 (s, I H) and 9.30 (s, 1H) ppm; MS (ES+) 466.2
Compound IA-303 5-(6-isopropylsulfonyl-3-pyridyl)-3-[5-[4-(methylaminomethyl)phenyl]- l ,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.24 (d, 6H), 2.31 (s, 3H), 3.76 (m, I H), 3.78 (s, 2H), 7.62 (d, 2H), 7.91 (br s, 2H), 8.14 - 8.20 (m, 3H), 8.81 (dd, I H), 9.15 (s, 1H) and 9.54 (d, I H) ppm; MS (ES+) 466.2
Compound IA-305 5-(3-chloro-4-isopropylsulfonyl-phenyl)-3-[5-[4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.24 (d, 6H), 2.30 (s, 3H), 3.77 (s, 2H), 3.79 (m, I H), 7.62 (d, 2H), 8.1 1 - 8.14 (m, 3H), 8.38 (dd, 1 H), 8.44 (d, 1 H) and 9.12 (s, 1 H) ppm; MS (ES+) 499.3
Compound IA-312 5-(4-isopropylsulfonyl-3-methyl-phenyl)-3-[5-[4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.20 (d, 6H), 2.33 (s, 3H), 2.74 (s, 3H), 3.50 (m, 1H), 3.82 (s, 2H), 7.64 (d, 2H), 7.96 - 7.98 (m, 1H), 8.14 (d, 2H), 8.20 - 8.23 (m, 2H) and 9.06 (s, 1H) ppm; MS (ES+) 479.3
Compound IA-314 5-(3-fluoro-4-isopropylsulfonyl-phenyl)-3-[5-[4- (methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.25 (d, 6H), 2.30 (s, 3H), 3.76 (s, 1H), 3.77 (s, 2H), 7.62 (d, 2H), 7.93 - 7.97 (m, 1H), 8.13 (d, 2H), 8.24 (s, 1H), 8.24 (dd, 1H) and 9.10 (s, 1H) ppm; MS (ES+) 483.2
Compound IA-316 5-(2-chloro-4-isopropylsulfonyl-phenyl)-3-[5-[4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 1.24 (d, 6H), 2.28 (s, 3H), 3.63 (t, 1H), 3.74 (s, 2H), 7.58 (d, 2H), 7.99 - 8.07 (m, 5H) and 8.71 (s, I H) ppm; MS (ES+) 499.2
Compound IA-322 5-[2-(difluoromethyl)-4-isopropylsulfonyl-phenyl]-3-[5-[4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 1.23 (d, 6H), 2.28 (s, 3H), 3.55-3.65 (m, I H), 3.75 (s, 2H), 7.59-7.62 (m, 3H), 8.06 (d, 2H), 8.16 (s, 2H), 8.20 (s, 1 H) and 8.80 (s, 1 H) ppm; MS (ES+) 515.3 Compound IA-326 5-(3-ethyl-4-isopropylsulfonyl-phenyl)-3-[5-[4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 1.21 (d, 6H), 1.34 (t, 3H), 2.68 (t, 3H), 3.09 (m, 2H), 3.45 (m, 1H), 4.29 (s, 2H), 7.77 (d, 2H), 7.97 (d, I H), 8.21 - 8.27 (m, 4H), 8.88 (s, 2H) and 9.1 1 (s, I H) ppm; MS (ES+) 493.3
Compound IA-331 2-[5-amino-6-[5-[4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2- yl]pyrazin-2-yl]-5-isopropylsulfonyl-benzonitrile 1H NMR (400.0 MHz, DMSO) d 1.23 (d, 6H), 2.29 (s, 3H), 3.66 (s, 1H), 3.75 (s, 2H), 7.60 (d, 2H), 8.13 (d, 2H), 8.24 (m, 1H), 8.38 - 8.42 (m, 2H) and 9.00 (s, 1H) ppm; MS (ES+) 490.1
Example 65A: 5-(4-isopropvlsulfonylphenyl)-3-r3-r2-methyl-4- (methylaminomethyl)phenyl]isoxazol-5-yl]pyrazin-2-amine (Compound IIA-12)
SCHEME
Figure imgf000326_0001
Compound IIA-12
Compound IIA-12 was prepared using Method IV-F, Steps 1-2, followed by Method IV- AAD, Steps 1-2, followed by Method IV-AAL, Stepl . METHOD IV-AAL
Step 1: 5-(4-isopropylsulfonylphenyl)-3-[3-[2-methyl-4-(methylaminomethyl)phenyl] isoxazol-5-yl]pyrazin-2-amine
[00531] TFA (556.9 mg, 376.3 μL, 4.884 mmol) was added to a solution of tert-butyl N- [[4-[5-[3-[bis(tert-butoxycarbonyl)amino]-6-(4-isopropylsulfonylphenyl)pyrazin-2- yl]isoxazol-3-yl]-3-methyl-phenyl]methyl]-N-methyl-carbamate (190 mg, 0.2442 mmol) in dichloromethane (4.750 mL) and the resulting yellow solution stirred overnight at room temperature. The reaction mixture was concentrated in vacuo and the residue taken up in methanol (2 mL) and dichloromethane (1 mL) and passed through an SCX cartridge and the product eluted with 2M ammonia in methanol and concentrated in vacuo. The filtrate was purified further by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were collected and lyopholised to give the product as a yellow solid (96.4 mg, 69% yield); 1H NMR (400.0 MHz, DMSO) d 1.18 (d, 6H), 2.61 (s, 3H), 2.62 (m, 3H), 3.48 (m, 1H), 4.20 (m, 2H), 7.24 (br s, 2H), 7.48-7.52 (m, 2H), 7.63 (s, I H), 7.84 (m, 1H), 7.93 (m, 2H), 8.37 (m, 2H), 8.81 (br s, 2H) and 8.97 (s, 1H) ppm; MS (ES+) 478.3
[00532] The following compounds were all prepared using a method similar to the one described for Compound HA- 12 above.
Compound IIA-13 3-[3-[3-chloro-4-(methylaminomethyl)phenyl]isoxazol-5-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.19 (m, 6H), 2.71 (s, 3H), 3.48 (m, 1 H), 4.37 (s, 2H), 7.24 (br s, 2H), 7.79 (m, 1 H), 7.95 (m, 2H), 8.12 (m, 1 H), 8.25 (m, 1 H), 8.38 (m, 2H) and 8.98 (br s, 2H) ppm; MS (ES+) 498.25
Compound IIA-14 3-[3-[2-fluoro-4-(methylaminomethyl)phenyl]isoxazol-5-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.18 (m, 6H), 2.63 (m, 3H), 3.47 (m, I H), 4.26 (m, 2H), 7.26 (br s, 2H), 7.51 (m, I H), 7.60 (m, 1H), 7.65 (m, 1 H), 7.94 (m, 2H), 8.13 (t, 1 H), 8.36 (m, 2H), 8.88 (br s, 2H) amd 8.98 (s, 1 H) ppm; MS (ES+) 482.0 Compound IIA-15 3-[3-[2-chloro-4-(methylaminomethyl)phenyl]isoxazol-5-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.18 (d, 6H), 2.63 (t, 3H), 3.49 (m, 1 H), 4.26 (m, 2H), 7.25 (br s, 2H), 7.63-7.65 (m, 2H), 7.85 (m, 1H),7.93 (m, 3H), 8.36 (m, 2H), 8.87 (br s, 2H) amd 8.98 (s, 1H) ppm; MS (ES+) 498.2
Example 66A: 5-(4-(ethvlsulfonyl*)phenvl)-3-C5-(3-('(methvlamino)methvl)phenvl)-1.3.4- oxadiazol-2-yl)pyrazin-2-amine (Compound IA-307)
SCHEME Method IV-AAM NH2 N-N Method IV-AAM N-N
Figure imgf000328_0001
Compound IA-307
Compound IA-307 was prepared using Method IV-AJ, Step 1 , followed by Method IV- AAM, Steps 1-3.
METHOD IV-AAM
Step 1: 5-bromo-3-(5-(4-(bromomethyl)phenyl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-amine
[00533] Dibromo(triphenyl)phosphorane (37.29 g, 88.35 mmol) was added to a suspension of 4-(bromomethyl)benzoic acid (4.318 g, 20.08 mmol) and 3-amino-6-bromo- pyrazine-2-carbohydrazide (4.66 g, 20.08 mmol) in acetonitrile (143.4 mL). The resulting mixture was stirred at room temperature for 2 h and then Hunig's base (15.57 g, 20.98 mL, 120.5 mmol) was added and the reaction was stirred overnight. Exotherm observed during Hunig's base addition; moderated with ice bath (temp, kept around 20 +/- 4). The reaction mixture was filtered and the solid obtained washed with cold acetonitrile to leave the product as a yellow solid (5.45 g, 66.7 % yield); 1H NMR (400.0 MHz, DMSO) d 4.82 (s, 2H), 7.72 (d, 2H), 7.80 (s, I H), 8.1 1 (d, 2H) and 8.45 (s, 1H) ppm; MS (ES+) 412.1.
Step 2: 5-bromo-3-(5-(4-((methylamino)methyl)phenyl)-1,3,4-oxadiazol-2-yl)pyrazin-2- amine
[00534] 5-bromo-3-(5-(4-(bromomethyl)phenyl)-l ,3,4-oxadiazol-2-yl)pyrazin-2-amine (100 mg, 0.2433 mmol) and Na2CO3 (77.36 mg, 0.7299 mmol) were suspended in and treated with methylamine (182.4 μL of 2 M, 0.36 mmol). The reaction was heated at 60 °C for 10 min and then additional methylamine (426.0 μL of 2 M, 0.86 mmol) was then added and the reaction heated at 60°C or another 10 min. The reaction was cooled, diluted with water (5 mL)and extracted into dichloromethane (3 x 5 mL). The organic layer was dried over Na2SO4, filtered and concentrated in vacuo to yield 5-bromo-3-[5-[4- (methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]pyrazin-2-amine (74.7 mg, 85.34% yield) as a yellow solid; MS (ES+) 362.3
Step 3: 5-(4-(ethylsulfonyl)phenyl)-3-(5-(4-((methylamino)methyl)phenyl)-1,3,4-oxadiazol- 2-yl)pyrazin-2-amine
[00535] To a 0.5-2.0 mL microwave vial 5-bromo-3-[5-[4-(methylaminomethyl)phenyl]- 1,3,4-oxadiazol-2-yl]pyrazin-2-amine (100 mg, 0.24 mmol), 4-(ethylsulfonyl)phenylboronic acid (56.72 mg, 0.265 mmol), dioxane (1 mL) and aqueous solution OfNa2CO3 (361.3 μL of 2M solution, 0.72 mmol) were added. Palladium; triphenylphosphane (13.91 mg, 0.012 mmol) was then added and the vial sealed. The reaction mixture was heated in the microwave at 150 °C for 30 min. After this time the reaction mixture was dilutied with DMSO (2 mL) and filtered before purification by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were collected and evaporated to dryness to give the product as a yellow solid (64.35 mg, 65 % yield); 1H NMR (400.0 MHz, DMSO) d 1.14 (t, 3H), 2.64 (s, 3H), 3.33-3.39 (m, 2H), 4.29 (s, 2H), 7.77 (d, 2H), 8.02 (d, 2H), 8.26 (d, 2H), 8.41 (d, 2H), 8.93 (s, 2H) and 9.09 (s, I H) ppm; MS (ES+) 451.0 [00536] The following compounds were all prepared using a method similar to the one described for Compound IA-307 above.
Compound IA-289 5-[4-(2-dimethylaminoethylsulfonyl)phenyl]-3-[5-[4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine MS (ES+) 494.0
Compound IA-296 4-[5-amino-6-[5-[4-(methylaminomethyl)phenyl]-1,3,4-oxadiazoI-2- yl]pyrazin-2-yl]-N,N-dimethyl-benzenesulfonamide 1H NMR (400.0 MHz, DMSO) d 2.64 (t, 3H), 2.67 (s, 6H), 4.28-4.30 (m, 2H), 7.76 (d, 2H), 7.88 (d, 2H), 8.26 (d, 2H), 8.40 (d, 2H), 8.92 (s, 2H) and 9.08 (s, 1H) ppm; MS (ES+) 466.0
Compound IA-297 5-[4-(azetidin-1-ylsulfonyl)phenyl]-3-[5-[4-(methylaminomethyl) phenyl]- l ,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 2.01 (td, 2H), 2.64 (s, 3H), 3.72 (t, 4H), 4.29 (s, 2H), 7.77 (d, 2H), 7.94 (d, 2H), 8.26 (d, 2H), 8.45 (d, 2H), 8.94 (s, 2H) and 9.10 (s, I H) ppm; MS (ES+) 478.0
Compound IA-301 3-[4-[5-amino-6-[5-[4-(methylaminomethy l)phenyl]- 1 ,3,4-oxadiazol-2- yl]pyrazin-2-yl]phenyl]sulfonylpropan-1-ol 1H NMR (400.0 MHz, DMSO) d 1.71 (dd, 2H), 2.64 (s, 3H), 3.28-3.45 (m, 4H), 4.29 (s, 2H), 4.68 (s, 1H), 7.77 (d, 2H), 8.02 (d, 2H), 8.27 (d, 2H), 8.41 (d, 2H), 8.90 (s, 2H) and 9.09 (s, 1H) ppm; MS (ES+) 481.0
Compound IA-302 3-[5-[4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5-(4- tetrahydrofuran-3-ylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 2.14- 2.20 (m, 2H), 2.64 (s, 3H), 3.66 (dd, 1H), 3.77 (dd, 1H), 3.86 (dd, 1H), 4.05 (dd, 1H), 4.23- 4.26 (m, 1H), 4.29 (s, 2H), 7.77 (d, 2H), 8.05 (d, 2H), 8.26 (d, 2H), 8.42 (d, 2H), 8.94 (s, 2H) and 9.09 (s, I H) ppm; MS (ES+) 493.0
Compound IA-304 4-[5-amino-6-[5-[4-(methylaminomethyl)phenyl]- 1 ,3,4-oxadiazol-2- yl]pyrazin-2-yl]-N-(2-hydroxyethyl)benzenesulfonamide 1H NMR (400.0 MHz, DMSO) d 2.63 (d, 3H), 2.84 (q, 2H), 3.39 (t, 2H), 4.29 (s, 2H), 7.74 (q, I H), 7.78 (s, 2H), 7.93 (d, 2H), 8.26 (d, 2H), 8.34 (d, 2H), 8.99 (s, 2H) and 9.05 (s, I H) ppm; MS (ES+) 482.0 Compound IA-308 3-[5-[4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5-[4- (oxetan-3-ylsulfonyl)phenyl]pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 2.64 (s, 3H), 4.29 (s, 2H), 4.77-4.82 (m, 4H), 4.96 (s, 1H), 7.77 (d, 2H), 8.05 (d, 2H), 8.26 (d, 2H), 8.41- 8.43 (m, 2H), 8.89 (s, 2H) and 9.09 (s, 1H) ppm; MS (ES+) 479.0
Compound IA-310 3-[5-[4-(methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]-5-(4- propyIsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 0.94 (t, 3H), 1.60 (q, 2H), 2.64 (s, 3H), 3.32-3.36 (m, 1H), 4.29 (s, 2H), 7.77 (d, 2H), 8.02 (d, 2H), 8.26 (d, 2H), 8.39-8.41 (m, 2H), 8.95 (d, 2H) and 9.08 (s, 1H) ppm; MS (ES+) 465.0
Compound IA-313 3-[5-[4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5-(4-sec- butylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 0.94 (t, 3H), 1.19 (d, 3H), 1.32-1.40 (m, 1H), 1.89-1.83 (m, 1H), 2.28 (d, 3H), 3.26-3.31 (m, I H), 3.76 (s, 2H), 7.61 (d, 2H), 7.98 (d, 2H), 8.12 (d, 2H), 8.40 (d, 2H) and 9.06 (s, 1H) ppm; MS (ES+) 479.0
Compound IA-288 3-[5-[4-(methylaminomethyl)phenyl]-l , 3,4-oxadiazol-2-yl]-5-(4- methylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 2.64 (s, 3H), 3.29 (s, 3H), 4.29 (s, 2H), 7.77 (d, 2H), 8.06 (d, 2H), 8.26 (d, 2H), 8.39-8.41 (m, 2H), 8.92 -(s, 2H) and 9.09 (s, 1H) ppm; MS (ES+) 437.0
Compound IA-323 3-[5-[4-(methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]-5-(4- tetrahydropyran-4-ylsulfonylphenyl)pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 1.52- 1.63 (m, 2H), 1 1.78 (d, 2H), 2.64 (t, 3H), 3.30 (dd, 2H), 3.57-3.64 (m, 1 H), 3.92 (dd, 2H), 4.28-4.30 (m, 2H), 7.77 (d, 2H), 7.98 (d, 2H), 8.26 (d, 2H), 8.41-8.43 (m, 2H), 8.91 (s, 2H) and 9.01 (s, 1H) ppm; MS (ES+) 507
Compound IA:324 5-[4-[2-(dimethylamino)-1-methyl-ethyl]sulfonylphenyl]-3-[5-[4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 1.30 (d, 3H), 2.64 (s, 3H), 2.81 (s, 3H), 2.90 (s, 3H), 3.35 (s, 1H), 3.56 (s, 1H), 4.09 (s, I H), 4.29 (s, 2H), 7.77 (d, 2H), 7.90-7.97 (m, 2H), 8.06 (d, 2H), 8.25 (d, 2H), 8.47 (d, 2H), 9.03 (s, 2H), 9.13 (s, I H) and 9.65 (s, 1H) ppm; MS (ES+) 508 Compound IA-328 4-[4-[5-amino-6-[5-[2-fluoro-4-(methylaminomethyl)phenyl]-1,3,4- oxadiazol-2-yl]pyrazin-2-yl]phenyl]sulfonyl-2-methyl-pentan-2-ol MS (ES+) 541
Compound IA-332 3-[5-[2-fluoro-4-(methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]-5- [4-(3-methoxy-1-methyl-propyl)sulfonylphenyl]pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.24 (d, 3H), 1.51-1.57 (m, I H), 2.07-2.14 (m, 1H), 2.67 (s, 3H), 3.22 (s, 3H), 3.40-3.45 (m, 3H), ), 4.33 (s, 2H), 7.62 (m, 1H), 7.72 (d, 1H), 8.02 (d, 2H), 8.31 (t, 1H), 8.40 (d, 2H), 9.06 (s, 2H) and 9.12 (s, 1 H) ppm; MS (ES+) 528
Compound IA-338 3-[5-[2-fluoro-4-(methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]-5- (4-sec-butylsulfonylphenyl)pyrazin-2-amine MS (ES+) 497
Compound IA-344 3-[5-[2-fluoro-4-(methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]-5- (4-tetrahydropyran-4-ylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.52-1.63 (m, 2H), 1.77 (d, 2H), 2.64 (s, 3H), 3.30 (dd, 2H), 3.56-3.64 (m, 1 H), 3.92 (dd, 2H), 4.31 (s, 2H), 7.60 (dd, 1H), 7.68-7.70 (m, 1H), 7.98 (d, 2H), 8.28 (t, 1H), 8.37-8.39 (m, I H), 8.98 (s, 2H) and 9.09 (s, I H) ppm; MS (ES+) 525
Compound IA-347 3-[5-[2-fluoro-4-(methylaminomethyl)phenyl]-1,3,4-oxadiazol-2-yl]-5- (4-tetrahydrofuran-3-ylsulfonylphenyl)pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 2.13-2.20 (m, 2H), 2.64 (s, 3H), 3.66 (dd, 1H), 3.74-3.80 (m, 1H), 3.86 (m, 1H), 4.04 (m, I H), 4.22-4.28 (m, 1H), 4.31 (s, 2H), 7.60 (dd, 1H), 7.69 (d, 1H), 8.05 (d, 2H), 8.29 (t, 1H), 8.38 (d, 2H), 8.96 (s, 2H) and 9.10 (s, 1H) ppm; MS (ES+) 51 1
Compound IA-330 5-[4-(3-methoxy-1-methyl-propyl)sulfonylphenyl]-3-[5-[4- (methylaminomethyl)phenyl]-l ,3,4-oxadiazol-2-yl]pyrazin-2-amine MS (ES+) 509
Example 67A: 2-r5-r3-amino-6-(4-isopropvlsulfonvlphenvπpyrazin-2-vπ-l .3.4-oxadiazol-2- yl]-5-(methylaminomethyl)phenol (Compound IA-291)
SCHEME
Method IV-C Steps 1 2
Figure imgf000333_0001
Compound IA-291
[00537] Compound IA-291 was prepared using Method IV-C, Steps 1 -2, followed by Method IV-AR, Steps 1-2, followed by Method IV-AAN, Step 1.
METHOD IV-AAN
Step 1: 2-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazol-2-yl]-5- (methy laminomethy 1 )phenol
[00538] To a solution of tert-butyl N-[[4-[5-[3-amino-6-(4-isopropylsulfonylphenyl) pyrazin-2-yl]-l ,3,4-oxadiazol-2-yl]-3-chloro-phenyl]methyl]-N-methyl-carbamate (130 mg, 0.2170 mmol) in dioxane (3 mL) was added of 1,5-diphenylpenta-1,4-dien-3-one; palladium (6.239 mg, 0.01085 mmol), di-tert-butyl-[2-(2,4,6-triisopropylphenyl)phenyl]phosphane (13.82 mg, 0.03255 mmol) and potassium hydroxide (434.0 μL of 1 M, 0.4340 mmol). The resulting mixture was heated to 100°C for 2 h. Additional 1,5-diphenylpenta-1,4-dien-3-one; palladium (6.239 mg, 0.01085 mmol), ditert-butyl-[2-(2,4,6-triisopropylphenyl)phenyl] phosphane (13.82 mg, 0.03255 mmol) and potassium hydroxide (434.0 μL of 1 M, 0.4340 mmol) were added and the resulting mixture heated for a further 2 h at 100°C. The reaction mixture was evaporated to dryness and the residue purified by column chromatography on silica eluting with 20% EtOAc/ petroleum ether. Product fractions were combined and concentrated in vacuo. This mixture was dissolved in DCM (10 mL) and TFA (247.4 mg, 167.2 μL, 2.170 mmol) added. The resulting mixture was stirred at room temperature for 1 h and then concentrated in vacuo to an oil. This was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were collected and lyopholised to give the product as a yellow solid (24.0 mg, 18 % yield); 1H NMR (400.0 MHz, DMSO) d 1.31 (m, 6H), 2.80 (s, 3H), 3.43 (m, 1H), 4.27 (s, 2H), 7.23 (m, 1H), 7.30 (m, 1H), 8.04 (m, 2H), 8.19 (m, 1H), 8.41 (m, 2H) and 8.95 (s, 1H) ppm; MS (ES+) 481.2
[00539] The following compounds were all prepared using a method similar to the one described for Compound 1-291 above.
Compound 1-320 5-[5-[3-amino-6-(4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4- oxadiazol-2-yl]-2-(methylaminomethyl)phenol 1H NMR (400.0 MHz, DMSO) d 1.3 (d, 6H), 2.7 (s, 3H), 3.4-3.5 (m, 1H),4.45 (s, 2H), 7.7 (d, 1H), 7.8-7.83 (m, 2H), 8.05 (d, 2H), 8.4 (d, 2H) and 8.95 (s, 1 H) ppm; MS (ES+) 481.2
Example 67A: 2-r5-amino-6-(5-phenvl-1.3.4-thiadiazol-2-vl')pvrazin-2-vll-5-(1,4-diazepane- l-carbonyl)benzonitrile (Compound IVA-2)
SCHEME
Figure imgf000334_0001
Compound IVA-2 was prepared using Method IV-D, Step 1, followed by Method IV-AAO, Step 1.
METHOD IV-AAO
Step 1: 2-[5-amino-6-(5-phenyl-l ,3,4-thiadiazol-2-yl)pyrazin-2-yl]-5-(1,4-diazepane-1- carbonyl)benzonitrile [00540] A mixture of methyl 4-bromo-3-cyano-benzoate (100 mg, 0.4166 mmol), potassium acetate (122.7 mg, 1.250 mmol), 4,4,5,5-tetramethyl-2-(4,4,5,5-tetramethyl-l ,3,2- dioxaborolan-2-yl)-1,3,2-dioxaborolane (158.7 mg, 0.6249 mmol) and l-cyclopenta-1 ,4- dienyl-diphenyl-phosphane; dichloromethane; dichloropalladium; iron (34.02 mg, 0.04166 mmol) was heated in dioxane (10 mL) at 80°C for 2 h. After this time, the reaction mixture was cooled and palladium; triphenylphosphane (48.14 mg, 0.04166 mmol), sodium carbonate (625.0 μL of 2 M, 1.250 mmol) and 5-bromo-3-(5-phenyl-1,3,4-thiadiazol-2-yl)pyrazin-2- amine (139.2 mg, 0.4166 mmol) were added and heated at 140°C under microwave conditions for Ih. After cooling to room temperature, the resulting carboxylic acid was filtered off as brown solid. The solid was dissolved in DMF (3 mL) and 1,4-diazepane (208.3 mg, 2.083 mmol) and TBTU (267.5 mg, 0.8332 mmol) were added. The resulting mixture was stirred at room temperature for 2 h then diluted with ethyl acetate (5 mL), and the organic extract washed with water (1 x 5 mL) and brine (1 x 5 mL), dried over MgSO4 and concentrated in vacuo to a solid. This solid was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were collected and lyopholised to give the product as a yellow solid (60 mg, 25 % yield); 1H NMR (400.0 MHz, DMSO) d 1.9-2.1 (m, 2H), 3.3-3.4 (m, 4H), 3.5-3.55 (m, 2H), 3.65-3.7 (m, I H), 3.7-3.75 (m, I H), 3.8-3.9 (m, 2H), 7.5-7.6 (m, 3H), 7.8 (d, I H), 8.1-8.22 (m, 3H), 8.25 (br s, 1 H), 8.75 (br s, 2H) and 8.8 (s, 1 H) ppm; MS (ES+) 483.2 [00541] The following compounds were all prepared using a method similar to the one described for Compound 1VA-2 above.
Compound IVA-I 4-[5-amino-6-(5-phenyl-1,3,4-thiadiazol-2-yl)pyrazin-2-yI]-3-cyano- N,N-dimethyl-benzamide 1H NMR (400.0 MHz, DMSO) d 3.0 (d, 6H), 7.6-7.65 (m, 3H), 7.85 (d, 1H), 8.1-8.2 (m, 4H), 8.25 (br s, 1H) and 8.8 (s, 1H) ppm; MS (ES+) 428.1
Example 68A: 4-r5-amino-6-15-(2-cvanoanilinoVl .3.4-thiadiazol-2-vllpvrazin-2-vll-N.N- dimethyl-benzamide (Compound IVA-3)
SCHEME
Figure imgf000336_0001
[00542] Compound IVA-3 was prepared using Method IV-C, Steps 1-2, followed by Method IV-AAP, Step 1.
METHOD IV-AAP
Step 1: 2-[5-amino-6-(5-phenyl-1,3,4-thiadiazol-2-yl)pyrazin-2-yl]-5-(1,4-diazepane-1- carbony l)benzonitri Ie
[00543] A mixture of 4-(5-amino-6-(hydrazinecarbonyl)pyrazin-2-y I)-N1N- dimethylbenzamide (75 mg, 0.2373 mmol), 2-isothiocyanatobenzonitrile (38.01 mg, 0.2373 mmol) in CH2CI2 (1.425 mL) was stirred at room temperature for 2 h. Ether was added and the reaction mixture filtered to give a yellow solid. This was taken up in anhydrous acetonitrile (1.5 mL) and then cooled in an ice bath. DIPEA (92.01 mg, 124.0 μL, 0.7119 mmol) was added, followed by portionwise addition of dibromo(triphenyl)phosphorane (130.2 mg, 0.3085 mmol). The resulting mixture was stirred at room temperature overnight and then heated under reflux for 1 h. The reaction mixture was cooled to room temperature and then filtered. The solid was washed further with acetonitrile (5 mL) and dried under vacuum to give the product as a bright yellow solid (68.0 mg, 62 % yield); 1H NMR (400.0 MHz, DMSO) d 3.01 (d, 6H), 5.76 (s, 2H), 7.55-7.60 (m, 3H), 7.73 (d, 1H), 7.83-7.87 (m, 2H), 8.18 (d, 2H), 8.43-8.45 (m, 1H) and 8.91 (s, 1H) ppm; MS (ES+) 443.17 Example 69A: 3-f3-r2-fluoro-4-(methvlaminomethvl')phenvl]isoxazol-5-yl]-5-('4- tetrahydrofuran-3-ylsulfonylphenyl)pyrazin-2-amine (Compound IIA-16)
SCHEME
Method IV-F Method IV-AAD Method IV-AAQ
Figure imgf000337_0001
Compound IIA-16 was prepared using Method IV-F, Steps 1-2, followed by Method IV- AAD, Steps 1-2, followed by Method IV-AAQ, Step 1.
METHOD IV-AAQ
Step 1: 3-[3-[2-fluoro-4-(methylaminomethyl)phenyl]isoxazol-5-yl]-5-(4-tetrahydrofuran-3- ylsulfonylphenyl)pyrazin-2-amine
TFA (281.6 mg, 190.3 μL, 2.470 mmol) was added to a solution of tert-butyl N-[[4-[5-[3- [bis(tert-butoxycarbonyl)amino]-6-(4-tetrahydrofuran-3-ylsulfonylphenyl)pyrazin-2- yl]isoxazol-3-yl]-3-fluoro-phenyl]methyl]-N-methyl-carbamate (100 mg, 0.1235 mmol) in dichloromethane (2.069 mL) at room temperature and the resulting solution stirred for 2h. The reaction mixture was concentrated in vacuo and purified by reverse phase preparative HPLC [Waters Sunfire Cl 8, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were collected and lyopholised to give the product as a yellow solid (34.0 mg, 44% yield); 1H NMR (400.0 MHz, DMSO) d 2.13-2.19 (m, 2H), 2.63 (m, 3H), 3.66 (m, 1H), 3.76 (m, 1H), 3.85 (m, 1H), 4.04 (m, 1H), 4.21-4.28 (m, 3H), 7.27 (m, 2H), 7.52 (m, 1H), 7.60 -7.65 (m, 2H), 8.00 (m, 2H), 8.12 (t, 1 H), 8.36 (m, 2H) and 8.94 (m, 3H) ppm; MS (ES+) 510.2 Example 7OA: 3-r5-r4-r(lSVl-amino-2.2.2-trifluoro-ethvllDhenvll-1.3.4-oxadiazol-2-vll-5- (4-isopropylsulfonylphenyl)pyrazin-2-amine (Compound IA-325)
SCHEME
Method IV-B Method IV-AAR
Figure imgf000338_0001
Compound IA-325
Compound IA-325 was prepared using Method IV-B, Steps 1-4, followed by Method IV- AAR, Step 1.
METHOD IV-AAR
Step l: 3-[5-[4-[(lS)-1-amino-2,2,2-trifluoro-ethyl]phenyl]-1,3,4-oxadiazol-2-yl]-5-(4- isopropylsulfonylphenyl)pyrazin-2-amine
HCl (35.27 μL of 3 M, 0.1058 mmol) was added to a solution of N-[(l S)-1-[4-[5-[3-amino-6- (4-isopropylsulfonylphenyl)pyrazin-2-yl]-1,3,4-oxadiazol-2-yl]phenyl]-2,2,2-trifluoro-ethyl]- 2-methyl-propane-2-sulfmamide (253.3 mg, 0.05288 mmol) in MeOH (1 mL) and the resulting solution stirred at room temperature overnight. The reaction mixture was concentrated to dryness under reduced pressure and the residue triturated with acetonitrile and filtered. The solid was taken up in a mixture of acetonitrile/water/MeOH and passed through the bicarbonate cartridge. The eluent was concentrated in vacuo and then triturated with acetonitrile to give the product as a yellow solid (26 mg, 99% yield); 1H NMR (400.0 MHz, DMSO) d 1.20 (d, 6H), 2.68 (d, 2H), 3.41 - 3.51 (m, 1H), 4.65 - 4.75 (m, 1H), 7.81 (d, 2H), 7.98 (d, 2H), 8.20 (d, 2H), 8.41 (d, 2H) and 9.08 (s, 1H) ppm; MS (ES+) 519.1
E^ampJeJlAL5-[4-[2-(dimethylamino)-1-methyl-ethyl]sulfonylphenyl]-3-[5-(2- fluorophenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-amine (Compound IA-337) SCHEME
Figure imgf000339_0001
Compound IA-337
Compound IA-337 was prepared using Method IV-AAS, Steps 1-2.
METHOD IV-AAS
Step 1: 5-bromo-3-(5-(2-fluorophenyl)-1,3,4-oxadiazol-2-yl)pyrazin-2-amine [00544] To a suspension of 2-fluorobenzohydrazide (2 g, 12.98 mmol), 3-amino-6-bromo- pyrazine-2-carboxylic acid (2.830 g, 12.98 mmol), and TBTU (5.002 g, 15.58 mmol) in DMF (20.00 mL) was added DIPEA (3.691 g, 4.974 mL, 28.56 mmol). The resulting mixture was stirred at room temperature for 2 h. The reation mixture was diluted with water (20 mL) and extracted with EtOAc (3 x 20 mL), the combined organic extracts were washed with water (3 x 20 mL) and brine (1 x 20 mL), dried over MgSO4 and concentrated in vacuo. The residue was then triturated with acetonitrile and filted and dried to give 3-amino-6-bromo-N'-(2- fluorophenylcarbonyl)pyrazine-2-carbohydrazide as an orange solid. This was taken up in MeCN (20.00 mL) and added bromo(triphenyl)phosphonium (5.331 g, 15.58 mmol) was added followed by DIPEA (3.691 g, 4.974 mL, 28.56 mmol). The reaction mixture was stirred for 30 min, and then filtered. The solid was washed with acetonitrile to give the product as a yellow solid (1.46 g, 67%); 1H ΝMR (400 MHz, DMSO) d 7.48-7.54 (m, 2H), 7.75 (m, 3H), 8.12 (m, 1H) and 8.45 (m, 1H) ppm; MS (ES+) 338.03
Step 2: 5-[4-[2-(dimethylamino)-1-methyl-ethyl]sulfonylphenyl]-3-[5-(2-fluorophenyl)- 1,3,4-oxadiazol-2-yl]pyrazin-2-amine
[00545] 2-(4-bromophenyl)sulfonyl-Ν,Ν-dimethyl-propan-l -amine (100 mg, 0.3233 mmol) was dissolved in dioxane (1.774 mL) and Bis(pinacolato)diboron (123.6 mg, 0.4866 mmol) and potassium acetate (95.50 mg, 0.9731 mmol) were added. The reaction was degassed and filled with nitrogen five times then Pd(dppf)Cl2.DCM (26.40 mg, 0.03233 mmol) was added and the reaction heated to 90 °C for 2 h. The reaction mixture was cooled to room temperature and N2 bubbled through for 10 min. Then 5-bromo-3-(5-phenyl-1,3,4- oxadiazol-2-yl)pyrazin-2-amine (102.9 mg, 0.3233 mmol) and an aqueous solution of Na2CO3 (485.0 μL of 2 M, 0.9699 mmol) was added. N2 was bubbled through for a further 10 min then Pd(PPh3)4 (37.47 mg, 0.03243 mmol) was added and the reaction mixture heated under microwave conditions at 150 °C for 30 min. The reaction mixture was passed through an SCX-2 cartridge eluting with MeCN/MeOH, washing wirh 200 mL, followed by 2M NH3 in MeOH with MeCN to elute the compound. Evaporation of the solvent gave a brown solid which was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were collected and lyopholised to give the product as a yellow solid (67.1 mg, 33 % yield); 1H NMR (400.0 MHz, DMSO) d 1.29 (d, 3H), 2.80 (s, 3H), 2.90 (s, 3H), 3.35 (d, 1H), 3.48 (d, 1H), 4.09 (s, 1H), 7.50-7.60 (m, 2H), 7.76-7.81 (m, 1H), 8.06 (d, 2H), 8.20 (m, 1H), 8.43 (d, 2H), 9.12 (s, 1H) and 9.41 (s, 1H) ppm; MS (ES+) 483
[00546] The following compounds were all prepared using a method similar to the one described for Compound IA-337 above.
Compound IA-327 3-[5-(2-fluorophenyl)-l ,3,4-oxadiazol-2-yl]-5-(4-tetrahydropyran-4- ylsulfonylphenyl)pyrazin-2-amine MS (ES+) 482
Compound IA-339 3-[4-[5-amino-6-[5-(2-fluorophenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2- yl]phenyl]sulfonylbutan-1-ol 1H NMR (400.0 MHz, DMSO) d 1.20 (d, 3H), 1.37-1.46 (m, 1H), 1.99-2.02 (m, I H), 3.38-3.43 (m, 2H), 3.52 (s, 1H), 4.66 (t, I H), 7.50-7.59 (m, 2H), 7.77 (d, 1H), 7.97 (d, 2H), 8.20 (s, 1H), 8.37 (d, 2H) and 9.08 (s, I H) ppm; MS (ES+) 470
Compound IA-343 5-[4-[3-(dimethylamino)-1-methyl-propyl]sulfonylphenyl]-3-[5-(2- fluorophenyl)-1,3,4-oxadiazol-2-yl]pyrazin-2-amine 1H NMR (400.0 MHz, DMSO) d 1.20 (d, 3H), 1.76-1.82 (m, 1H), 2.14-2.20 (m, 1H), 2.79 (s, 6H), 3.21 (s, 2H), 3.47-3.55 (m, 1H), 7.50-7.59 (m, 2H), 7.75-7.81 (m, 1H), 8.01 (d, 2H), 8.19 (m, 1H), 8.40 (d, 2H), 9.10 (s, 1H) and 9.58 (s, 1H) ppm; MS (ES+) 497 Compound IA-349 3-[5-(2-fluorophenyl)-1,3,4-oxadiazol-2-yl]-5-(4-tetrahydrofuran-3- ylsulfonylphenyl)pyrazin-2-amine I H NMR (400.0 MHz, DMSO) d 2.13-2.19 (m, 2H), 3.65 (m, I H), 3.74-3.80 (m, I H), 3.86 (dd, I H), 4.04 (dd, I H), 4.22-4.28 (m, 1H), 7.50-7.60 (m, 2H), 7.75-7.80 (m, I H), 8.04 (d, 2H), 8.20 (m, 1HO, 8.38 (d, 2H) and 9.09 (s, 1H) ppm; MS (ES+) 468
Example 72A: 3-f3-f4-(Ydimethvlamino)methvO-2-fluorophenvl)isoxazol-5-vO-5-(3-fluoro-
4-(isopropylsulfonyl)phenyl)pyrazin-2-amine (Compound IIA-17)
SCHEME
Figure imgf000341_0001
Compound IIA-17
Compound IIA-17 was prepared using Method IV-F, Steps 1-4, followed by Method IV- AAT, Step 1.
tert-Butyl N-[[4-[5-[3-[bis(tert-butoxycarbonyl)amino]-6-bromo-pyrazin-2-yl]isoxazol-3-yl]- 3-fluoro-phenyl]methyl]-N-methyl-carbamate (150 mg, 0.221 1 mmol), 2-(3-fluoro-4- isopropylsulfonyl-phenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (72.57 mg, 0.2211 mmol) and Na2CO3 (46.87 mg, 0.4422 mmol) suspended in MeCN (2.486 mL) / water (2.486 mL) . Mixture de-gassed (x5 N 2 -vacuum cycles) and Pd(PPh3^ (25.55 mg, 0.0221 1 mmol) added. Mixture de-gassed again and the reaction mixture was heated at 90 °C under microwave conditions for 20 min. The reaction mixture was diluted with water (5 mL) and ethyl acetate (5 mL) and the layers separated. The aqueous layer wasextracted further with ethyl acetate (2 x 10 mL) and the combined organic extracts dried over MgSθ4 and concentrated in vacuo. The residue was taken up in dichloromethane (3 mL) and TFA (504.2 mg, 340.7 μL, 4.422 mmol) was added. The resulting solution was stirred at room temperature for 4 h and then concentrated in vacuo. The residue was purified by reverse phase preparative HPLC [Waters Sunfire C 18, 10 μM, 100 A column, gradient 10% - 95% B (solvent A: 0.05% TFA in water; solvent B: CH 3 CN) over 16 minutes at 25 mL/min]. The product fractions were collected and lyopholised to give the product as a yellow solid (47.5 mg, 45% yield); MS (ES+) 500.1
[00547] Compounds P73 to P144, P145, P147-P148 and Pl 50 can be made using a methodology similar to the one described in Method AAT.
[0001] The compounds of Table A were synthesized using the methods described herein as well as those known in the art. More specifically, the compounds were made using one or more of the following methods: Oxadiazolyl compounds (Formula IA compounds) can be made according to methods described in Schemes I-B2 and I-B3; Isoxazolyl compounds (Formula Il A compounds) can be made according to methods described in Schemes I-El and I-E2. Triazolyl compounds (Formula INA) can be made according to methods described in Schemes I-Fl and I-F2.
Table A
Figure imgf000342_0001
Figure imgf000343_0001
Figure imgf000344_0001
Figure imgf000345_0001
Figure imgf000346_0001
Figure imgf000347_0001
Figure imgf000348_0002
Example 73A : 3-amino-Λ^-(lH-benzo[</]imidazol-2-yD-6-(4-(dimethvlcarbamovD phenyl)pyrazine-2-carboxamide (Compound V-I)
SCHEME
Figure imgf000348_0001
Compound V-I was prepared using Method V-A, Steps 1-2
METHOD V-A:
Step 1 : 3-Amino-6-(4-(dimethylcarbamoyl)phenyl)pyrazine-2-carboxylic acid [00548] Methyl 3-amino-6-bromo-pyrazine-2-carboxylate (6.01 g, 25.9 mmol, 1.0 Eq.), 4- (dimethylcarbamoyl)phenylboronic acid (5.00 g, 25.9 mmol, 1.0 Eq.), Na2CO3 (5.49 g, 51.8 mmol, 2.0 Eq.) and Pd(PPh3)4 (2.99 g, 2.59 mmol, 0.1 Eq.) in acetonitrile (30 mL) and water (30 mL) were heated at 90 °C for 16 hours. After cooling to ambient temperature the precipitate was removed by filtration. The aqueous filtrate was acidified to pH4 by addition of IM HCl and then extracted with dichloromethane (3 x 20 mL), dried over MgSO4 and concentrated in vacuo to give the sub-title product as a yellow solid (2.42 g, 65% Yield). 1H NMR (400.0 MHz, DMSO) δ 2.95 (3H, br s), 3.00 (3H, br s), 7.49-7.51 (2H, m), 7.58 (2H, br s), 8.15 92H, d), 8.95 (I H, s),13.25 (1H, br s) ppm; MS (ES+) 287.13. Step 2 : 3-amino-N-(lH-benzo[<aT|imidazol-2-yl)-6-(4-(dimethylcarbamoyl) phenyl)pyrazine- 2-carboxamide
[00549] Amino-6-(4-(dimethylcarbamoyl)phenyl)pyrazine-2-carboxylic acid (1 12.5 mg, 0.3930 mmol, 1.0 Eq.) in DMF (1.1 mL) was treated with lΗ-benzimidazol-2-amine (62.8 mg, 0.4716 mmol, 1.2 Eq.) and triethylamine (39.8 mg, 54.8 μL, 0.3930 mmol, 1.0 Eq.) followed by the addition of TBTU (176.7 mg, 0.5502 mmol, 1.4 Eq.). The reaction mixture was allowed to stir at ambient temperature overnight then added dropwise to stirred water (15ml). This was stirred at ambient temperature for 1 hour and the resultant precipitate isolated by filtration and washed with water. The residue was recrystallised from hot acetonitrile to give the title compound as a yellow solid (63.1 mg, 40% Yield). 1 H ΝMR (400.0 MHz, DMSO) δ 2.97 (3H, br s), 3.02 (3H, br s), 7.15-7.18 (2H, m), 7.51-7.55 (4H, m), 7.83 (2H, br s), 8.34 (2H, d), 9.04 (1H, s), 1 1.50 (1H, br s), 12.35 (1H, br s) ppm; MS (ES+) 402.08.
[00550] Compounds V-I to V-30 can also be prepared using a method similar to the one used to prepare compound V-I .
Figure imgf000349_0001
Figure imgf000350_0001
Figure imgf000351_0001
[0002] The compounds of Table B were synthesized using the methods described herein as well as those known in the art. More specifically, the compounds were made using one or more of the following methods: benzothiazolyl compounds can be made according to methods described in Schemes I-Hl and I-H2; benzoxazolyl compounds can be made according to methods described in Schemes I-Gl . Benzimidazolyl compounds can be made according to methods described in Schemes I— 11 and I-I2. Heteroaromatic amides can be made according to methods described in Schemes I-Al and I-A2. Table B
Figure imgf000352_0001
Figure imgf000353_0001
Figure imgf000354_0001
Examples 75A: Cellular ATR Inhibition Assay:
[00551] Compounds can be screened for their ability to inhibit intracellular ATR using an immunofluorescence microscopy assay to detect phosphorylation of the ATR substrate histone H2AX in hydroxyurea treated cells. HT29 cells are plated at 14,000 cells per well in 96-well black imaging plates (BD 353219) in McCoy's 5A media (Sigma M8403) supplemented with 10% foetal bovine serum (JRH Biosciences 12003), Penicillin/Streptomycin solution diluted 1 : 100 (Sigma P7539), and 2mM L-glumtamine (Sigma G7513), and allowed to adhere overnight at 37°C in 5% CO2. Compounds are then added to the cell media from a final concentration of 25μM in 3-fold serial dilutions and the cells are incubated at 37°C in 5% CO2. After 15min, hydroxyurea (Sigma H8627) is added to a final concentration of 2mM.
[00552] After 45min of treatment with hydroxyurea, the cells are washed in PBS, fixed for lOmin in 4% formaldehyde diluted in PBS (Polysciences Inc 18814), washed in 0.2% Tween-20 in PBS (wash buffer), and permeabilised for lOmin in 0.5% Triton X-100 in PBS, all at room temperature. The cells are then washed once in wash buffer and blocked for 30min at room temperature in 10% goat serum (Sigma G9023) diluted in wash buffer (block buffer). To detect H2AX phosphorylation levels, the cells are then incubated for Ih at room temperature in primary antibody (mouse monoclonal anti-phosphorylated histone H2AX Serl39 antibody; Upstate 05-636) diluted 1 :250 in block buffer. The cells are then washed five times in wash buffer before incubation for Ih at room temperature in the dark in a mixture of secondary antibody (goat anti-mouse Alexa Fluor 488 conjugated antibody; Invitrogen Al 1029) and Hoechst stain (Invitrogen H3570); diluted 1 :500 and 1 :5000, respectively, in wash buffer. The cells are then washed five times in wash buffer and finally lOOul PBS is added to each well before imaging.
[00553] Cells are imaged for Alexa Fluor 488 and Hoechst intensity using the BD Pathway 855 Bioimager and Attovision software (BD Biosciences, Version 1.6/855) to quantify phosphorylated H2AX Serl39 and DNA staining, respectively. The percentage of phosphorylated H2AX-positive nuclei in a montage of 9 images at 2Ox magnification is then calculated for each well using BD Image Data Explorer software (BD Biosciences Version 2.2.15). Phosphorylated H2AX-positive nuclei are defined as Hoechst-positive regions of interest containing Alexa Fluor 488 intensity at 1.75-fold the average Alexa Fluor 488 intensity in cells not treated with hydroxyurea. The percentage of H2AX positive nuclei is finally plotted against concentration for each compound and IC50s for intracellular ATR inhibition are determined using Prism software(GraphPad Prism version 3.0cx for Macintosh, GraphPad Software, San Diego California, USA).
[00554] The compounds described herein can also be tested according to other methods known in the art (see Sarkaria et al, "Inhibition of ATM and ATR Kinase Activities by the Radiosensitizing Agent, Caffeine: Cancer Research 59: 4375-5382 (1999); Hickson et al, "Identification and Characterization of a Novel and Specific Inhibitor of the Ataxia- Telangiectasia Mutated Kinase ATM" Cancer Research 64: 9152-9159 (2004); Kim et al, "Substrate Specificities and Identification of Putative Substrates of ATM Kinase Family Members" The Journal of Biological Chemistry, 274(53): 37538-37543 (1999); and Chiang et al, "Determination of the catalytic activities of mTOR and other members of the phosphoinositide-3-kinase-related kinase family" Methods MoI. Biol. 281 : 125-41 (2004)).
Example 76A: ATR Inhibition Assay:
[00555] Compounds were screened for their ability to inhibit ATR kinase using a radioactive-phosphate incorporation assay. Assays were carried out in a mixture of 5OmM Tris/HCl (pH 7.5), 1OmM MgCl2 and ImM DTT. Final substrate concentrations were 10μM [γ-33P]ATP (3mCi 33P ATP/mmol ATP, Perkin Elmer) and 800 μM target peptide (ASELPASQPQPFSAKKK).
[00556] Assays were carried out at 25°C in the presence of 5 nM full-length ATR. An assay stock buffer solution was prepared containing all of the reagents listed above, with the exception. of ATP and the test compound of interest. 13.5 μL of the stock solution was placed in a 96 well plate followed by addition of 2 μL of DMSO stock containing serial . dilutions of the test compound (typically starting from a final concentration of 15 μM with 3- fold serial dilutions) in duplicate (final DMSO concentration 7%). The plate was pre- incubated for 10 minutes at 25°C and the reaction initiated by addition of 15 μL [γ-33P]ATP (final concentration 10 μM).
[00557] The reaction was stopped after 24 hours by the addition of 30μL 0.1 M phosphoric acid containing 2mM ATP. A multiscreen phosphocellulose filter 96-well plate (Millipore, Cat no. MAPHN0B50) was pretreated with lOOμL 0.2M phosphoric acid prior to the addition of 45μL of the stopped assay mixture. The plate was washed with 5 x 200μL 0.2M phosphoric acid. After drying, 100 μL Optiphase 'SuperMix' liquid scintillation cocktail (Perkin Elmer) was added to the well prior to scintillation counting (1450 Microbeta Liquid Scintillation Counter, Wallac).
[00558] After removing mean background values for all of the data points, Ki(app) data were calculated from non-linear regression analysis of the initial rate data using the Prism software package (GraphPad Prism version 3.0cx for Macintosh, GraphPad Software, San Diego California, USA).
[00559] Below is a chart showing the ATR Inhibition Ki values of compounds of the disclosure. Compounds with a Ki value of < 10 nM are marked with "+++." Compounds with a Ki value > 10 nM but < 100 nM are marked with "++." Compounds with a Ki value > 100 nM but < 5uM are marked with "+."
PERlKi
1-7 ++
1-8 +
1-9 + 1-10 1-11
Figure imgf000356_0001
1-12 +
Figure imgf000356_0002
Figure imgf000357_0001
Figure imgf000357_0002
Figure imgf000357_0003
IA-43 ++
IA-44 ++
IA-45
IA-46 ++
IA-47 +++
IA-48 ++
IA-49 ++
IA-50 ++
IA-51 ++
IA-52 +++
IA-53 ++
IA-54 +
IA-55 +
IA-56 +
IA-57 +
IA-58 ++
IA-59 ++
IA-60 +++
IA-61 ++
IA-62 ++
IA-63 ++
IA-64 ++ IA-65 IA-66 ++ IA-67 ++ IA-68 +++ IA-69 ++ IA-70 +++ IA-71 +++ IA-72 + IA-73 +++ IA-74 +++ IA-75 ++ IA-76 +++ IA-77 +++ IA-78 ++ IA-79 ++ IA-80 +++ IA-81 IA-82 IA-83 +++ IA-84 +++ IA-85 ++
Figure imgf000358_0001
IA-86 +
Figure imgf000358_0002
Figure imgf000359_0001
Figure imgf000359_0002
Figure imgf000359_0003
Figure imgf000360_0001
Figure imgf000360_0002
Figure imgf000360_0003
Figure imgf000361_0002
Figure imgf000361_0001
Example 77A- Cisplatin Sensitization Assay
[00560] Compounds were screened for their ability to sensitize HCTl 16 colorectal cancer cells to Cisplatin using a 96h cell viability (MTS) assay. HCTl 16 cells, which possess a defect in ATM signaling to Cisplatin (see, Kim et al.; Oncogene 21 :3864 (2002); see also, Takemura et al.; JBC 281 :30814 (2006)) were plated at 470 cells per well in 96-well polystyrene plates (Costar 3596) in 150μl of McCoy's 5A media (Sigma M8403) supplemented with 10% foetal bovine serum (JRH Biosciences 12003), Penicillin/Streptomycin solution diluted 1 :100 (Sigma P7539), and 2mM L-glumtamine (Sigma G7513), and allowed to adhere overnight at 37°C in 5% CO2. Compounds and Cisplatin were then both added simultaneously to the cell media in 2-fold serial dilutions from a top final concentration of lOμM as a full matrix of concentrations in a final cell volume of 200μl, and the cells were then incubated at 37°C in 5% CO2. After 96h, 40μl of MTS reagent (Promega G358a) was added to each well and the cells were incubated for Ih at 37°C in 5% CO2. Finally, absorbance was measured at 490nm using a SpectraMax Plus 384 reader (Molecular Devices) and the concentration of compound required to reduce the IC50 of Cisplatin alone by at least 3-fold (to 1 decimal place) was reported. [00561] Compounds with an IC50 or Ki value of < 100 nM are marked with "+++." Compounds with an IC50 or Ki value > 100 nM but < 1 uM are marked with "++." Compounds with an IC50 or Ki value > 1 uM but < 20 uM are marked with "+."
Table C
Figure imgf000362_0001
[00562] While we have described a number of embodiments of this invention, it is apparent that our basic examples may be altered to provide other embodiments that utilize the compounds, methods, and processes of this invention. Therefore, it will be appreciated that the scope of this invention is to be defined by the appended claims rather than by the specific embodiments that have been represented by way of example herein.

Claims

We claim:
1. A compound of formula IA:
Figure imgf000364_0001
IA or a pharmaceutically acceptable salt thereof; wherein Y is a C1-C1oaliphatic chain wherein up to three methylene units of the aliphatic chain are optionally replaced with O, NR0, S, C(O) or S(O)2; Ring A is a 5 membered heteroaryl ring selected from
Figure imgf000364_0002
J3 is H or C1-4alkyl wherein 1 methylene unit of the alkyl group can optionally be replaced with O, NH, N(C1-4alkyl), or S and optionally substituted with 1-3 halo;
Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R5 is H; a 3-7 membered monocyclic fully saturated, partially unsaturated, or aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; an 8- 10 membered bicyclic fully saturated, partially unsaturated, or aromatic ring containing 0- 6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R5 is optionally substituted with 1-5 J5 groups;
L is a C1-4alkyl chain wherein up to two methylene units of the alkyl chain are optionally replaced with O, NR6, S, -C(O)-, -SO-, or -SO2-;
R0 is H or C1-C6alkyl wherein one methylene unit of the alkyl chain can be optionally replaced with O, NH, N(d.4alkyl), or S;
R1 is H or C1-C6alkyl; R2 is H, C1-Cόalkyl, -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said heterocyclic ring is optionally substituted with one occurrence of Jzl;
Jzι is halo, CN, C1-C8aliphatic, -(X)1-CN, or -(X)r-Z; wherein said up to two methylene units of said C1-C8aliphatic can be optionally replaced with O, NR, S, P(O), C(O), S(O), or S(O)2; wherein said C1-C8aliphatic is optionally substituted with halo, CN, Or NO2;
X is C1-C4alkyl; each t, r and m is independently O or 1 ;
Z is -NR3R4;
R3 is H or C1-C2alkyl;
R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said ring is optionally substituted with one occurrence of J ; R6 is H, or C1-Cealkyl; Jz is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH,
O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic), CO2(C1-4aliphatic),
0(haloC1-4aliphatic), or haloC1-4aliphatic; J5 is halo, oxo, CN, NO2, X'-R, or -(X')p-Q4;
XI is C1-10aliphatic; wherein 1-3 methylene units of said C1-10aliphatic are optionally replaced with -NR'-, -0-, -S-, C(=NR'), C(O), S(O)2, or S(O); wherein X1 is optionally and independently substituted with 1-4 occurrences OfNH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO2(C1-4aliphatic), C(O)NH2, C(O)NH(C1- 4aliphatic), C(O)N(C1-4aliphatic)2, SO(C1-4aliphatic), SO2(C1-4aliphatic), SO2NH(C1-4aliphatic), SO2NH(C1-4aliphatic), NHC(O)(C1-4aliphatic), N(C1-4aliphatic)C(O)(C1-4aliphatic), wherein said C1-4aliphatic is optionally substituted with 1-3 occurrences of halo;
Q4 is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 8-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q4 is optionally substituted with 1-5 J^4; Jv-* is halo, CN, or C1-4alkyl wherein up to 2 methylene units are optionally replaced with O, NR*, S, C(O), S(O), or S(O)2;
R is H or C1-4alkyl wherein said C1-4alkyl is optionally substituted with 1 -4 halo;
J2 is halo; CN; a 5-6 membered aromatic or nonaromatic monocyclic ring having 0-3 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C1 -10aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; wherein said C1-10aliphatic group is optionally substituted with 1-3 halo or CN; and said monocyclic ring is optionally substituted with 1-3 occurences of halo; CN; a C3-6Cycloalkyl; a 3-7 membered heterocyclyl containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C1-4alkyl wherein up to one methylene unit of the alkyl chain is optionally replaced with O, NR", or S; and wherein said C1-4alkyl is optionally substituted with 1-3 halo; q is O, 1, or 2; p is O or 1 ;
R', R", and R* are each independently H, C1-4alkyl, or is absent; wherein said C1-4alkyl is optionally substituted with 1 -4 halo.
2. The compound of claim 1 , wherein
Y is a C1-Cόaliphatic chain wherein one methylene unit of the alkyl chain is optionally replaced with C(O) or -NR0-;
R5 is a 3-7 membered monocyclic fully saturated, partially unsaturated, or aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic fully saturated, partially unsaturated, or aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R5 is optionally substituted with 1-5 J5 groups;
JZ1 is -(X)1-CN, C1-Cealkyl or -(X)^Z;
X1 is C1-10aliphatic; wherein 1-3 methylene units of said C1 -10aliphatic are optionally replaced with -NR'-, -O-, -S-, C(O), S(O)2, or S(O); wherein X1 is optionally and independently substituted with 1 -4 occurrences OfNH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(d.4aliphatic), NO2, CN, CO2H, CO2(C1-4aliphatic), C(O)NH2, C(O)NH(C1-4aliphatic), C(O)N(C i.4aliphatic)2, SO(C1-4aliphatic), SO2(C |.4aliphatic), SO2NH(C i .4al iphatic), SO2NH(C i _4al iphatic), NHC(O)(C i -4al iphatic), N(C1-4aliphatic)C(O)(C1-4aliphatic), wherein said C1-4aliphatic is optionally substituted with 1 -3 occurrences of halo.
3. The compound of claim 1 or claim 2, wherein J3 is H.
4. The compounds of any one of claims 1-3, wherein ring A is
Figure imgf000367_0001
5. The compounds of any one of claims 1-3, wherein ring A i
Figure imgf000367_0002
6. The compound of any one of claims 1-5, wherein Y is NH;
R5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R5 is optionally fused to a
5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R5 is optionally substituted with 1-5 J5 groups; L iS-C(O)- Or -SO2-; R1 is H, or C1-C6alkyl; R2 is -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of Jzι; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)1-Z; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said ring is optionally substituted with one occurrence of Jz; J5 is halogen, NO2, CN, O(haloC1-4aliphatic), haloC1-4aliphatic, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'; and J2 is halo, C|-C2alkyl optionally substituted with 1-3 fluoro, or CN.
7. The compound of claim 6, wherein L is -C(O)-, m is O, and R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1 - 2 nitrogen atoms.
8. The compound of claim 7, wherein said heterocyclyl is pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl.
9. The compound of any one of claims 1-6, wherein m is 0, q is 0, and -L-NR1R2 is C(O)pyrrolidinyl, C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O) 1,4-diazepanyl, C(O)NH-piperidinyl, C(O)NHCH2CH2-pyrrolidinyl, C(O)NHCH2CH2-piperidinyl, CON(CH3)CH2CH2N(CH3)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl or 1 ,4-diazepanyl is optionally substituted with C1-4alkyl or N(C1-4alkyl)2.
10. The compound of any one of claims 1-9, wherein J2 is halo, CN, phenyl, oxazolyl, or a C1-όaliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN.
1 1. The compound of claim 3 having Formula IIA:
Figure imgf000368_0001
HA wherein
Ring A is a 5 membered heteroaryl ring selected from
Figure imgf000368_0002
Y is a C1-C4alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with -NR0-;
Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
R5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R3 is optionally substituted with 1-5 J5 groups; L is-C(O)- or -SO2-; R1 is H, or C1-C6alkyl; R° is H or C1-C6alkyl; R2 is C1-C6alkyl, -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen, sulfur, or oxygen; wherein said heterocyclic ring is optionally substituted with one occurrence of Jzι; Jzι is (X)1-CN, C1-Cealkyl or -(X)1-Z; X is C1-C4alkyl; each t, r and m is independently 0 or 1 ; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said ring is optionally substituted with one occurrence of Jz; Jz is NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic),
NO2, CN, CO2H, CO(C1-4aliphatic), CO2(C1-4aliphatic), 0(haloC1-4aliphatic), or haloC1-4aliphatic; J5 is halogen, NO2, CN, O(haloC1-4aliphatic), haloC1-4aliphatic, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'; J2 is halo; CN; phenyl; oxazolyl; or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN; R' and R" are each independently H or C1-C4 alkyl; q is O, 1 , or 2, p is O or 1.
12. The compound of any one of claims 1-1 1 , wherein Q is phenyl or pyridyl.
13. The compound of claim 1 1 , selected from the following: Table IIA-2 (part 1)
Figure imgf000370_0002
14. The compound of claim 1 1 selected from the following:
Table IIA-2 (part 2)
Figure imgf000370_0001
Figure imgf000371_0001
15. The compound of claim 1 having Formula IA':
Figure imgf000372_0001
IA' or a pharmaceutically acceptable salt thereof; wherein Y is a C1-C4alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with -NR0-; G is O or S; Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R5 is a 3-7 membered monocyclic fully saturated, partially unsaturated, or aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an
8-10 membered bicyclic fully saturated, partially unsaturated, or aromatic ring containing
0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; R is optionally substituted with 1-5 J5 groups; L is C1-4alkyl chain wherein up to two methylene units of the alkyl chain are optionally replaced with O, NR6, S, -C(O)-, -SO-, or -SO2-; R0 is H or C1-C6alkyl; R1 is H or C1-Cealkyl; R2 is H, C1-C6alkyl, -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen, sulfur, or oxygen; wherein said heterocyclic ring is optionally substituted with one occurrence of J ; Jzι is -(X)1-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, r and m is independently 0 or 1 ; Z is -NR3R4; RJ is H or C1-C2alkyl;
R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said ring is optionally substituted with one occurrence of Jz;
R6 is H, or C1-C6alkyl;
Jz is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, 0(C1-4al iphatic), NO2, CN, CO2H, CO(C1-4aliphatic), CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1 -4aliphatic;
J5 is halo, oxo, CN, NO2, X '-R, or -(X')p-Q4,
X1 is C1-10aliphatic; wherein 1-3 methylene units of said C1-iOaliphatic are optionally replaced with -NR'-, -0-, -S-, C(O), S(O)2, or S(O); wherein X1 is optionally and independently substituted with 1-4 occurrences OfNH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO2(C1-4aliphatic), C(O)NH2, C(O)NH(C1-4aliphatic), C(O)N(C i.4aliphatic)2, SO(C1-4aliphatic), SO2(C1-4aliphatic), SO2NH(C i .4al iphatic), SO2NH(C i .4al iphatic), NHC(O)(C i .4al iphatic), N(C1-4aliphatic)C(O)(C1-4aliphatic), wherein said C1-4aliphatic is optionally substituted with 1 -3 occurrences of halo;
Q4 is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 8-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q4 is optionally substituted with 1-5 JQ4;
JQ4 is halo, CN, or C1-4alkyl wherein up to 2 methylene units are optionally replaced with O, NR*, S, C(O), S(O), or S(O)2;
R is H or C1-4alkyl wherein said C1-4alkyl is optionally substituted with 1-4 halo;
J2 is halo; CN; a 5-6 membered aromatic or nonaromatic monocyclic ring having 0-3 heteroatoms selected from oxygen, nitrogen, or sulfur; or a C1-iOaliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; wherein said C1-10aliphatic group is optionally substituted with 1-3 halo or CN; and said monocyclic ring is optionally substituted with 1-3 halo; CN; a C3.6cycloalkyl; a 3-7 membered heterocyclyl containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur or a C1-4alkyl wherein up to one methylene unit of the alkyl chain is optionally replaced with O, NR", or S; q is O, 1 , or 2, p is 0 or 1 ; and
R', R", and R* are each independently H, C).4alkyl, or is absent; wherein said C1-4alkyl is optionally substituted with 1-4 halo.
16. The compound of claim 15, wherein J5 is halo, CN, phenyl, oxazolyl, or a C1 -6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR', C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN.
17. The compound according to any one of claims 1-16, wherein p is O.
18. The compound according to any one of claims 17, wherein Q is phenyl, indolyl, pyridyl, naphthyl or benzothienyl, or quinolinyl.
19. The compound according to claim 18, wherein Q is phenyl or pyridyl.
20. The compound according to claim 19, wherein J2 is -OCH3, -OCH2CH2N(CH3)2, -NHCH2CH2N(CH3)2, or piperazinyl.
21. The compound according to claim 19, wherein Q is substituted in the ortho position, the para position, or in both the ortho and the para position.
22. The compound according to claim 19, wherein Q is substituted at the para position with J2, wherein the J2 is a C1-6aliphatic group wherein the methylene group bonded to Ring Q is replaced with -SO2-.
23. The compound of claim 22, wherein said 3-6 membered heterocyclyl is tetrahydrofuranyl, tetrahydropyranyl, azetidinyl, pyrrolidinyl, or piperidinyl.
24. The compound of claim 19 wherein at least one more methylene unit of the C1-6aliphatic group is optionally replaced with a heteroatom selected from the group consisting of O, NR", and S.
25. The compound according to claim 19, wherein Q is substituted at the para position with -SO2(C1-4alkyl), -SO2(C1-4alkyl)N(C1-4alkyl)2, C(O)N(C1-4alky I)2, C(O)(1 , 4-diazepanyl), CO(azepanyl), C(O)(piperazinyl), or C(O)(piperidinyl).
26. The compound of claim 21 , wherein at least one more methylene unit of the C1-6aliphatic group is optionally replaced with a heteroatom selected from the group consisting of O, NR", and S.
27. The compound according to claim 19, wherein Q is phenyl.
28. The compound according to claim 27, wherein Q is optionally substituted in the ortho- position with one J2, wherein J2 is C1-C4alkyl, NH2, NHC(O)CH3, O(C1-C4alkyl), CH2OH, CH2OCH3, CH2CH2OCH3, CH2CN, CN, CH2C(O)NH2, OH, OCF3, CF3, CHF2, -CH=CHP, NHC(O)CH3, C(O)CH3, C(O)NH2, SCH3, S(O)CH3, S(O)CH2CH3, SO2CH(CH3)2, -C^CH2, oxazolyl, or phenyl.
29. The compound of claim 28, wherein Q is substituted in the ortho position with J2, wherein J2 is CH2OH, CHF2, S(O)CH3, or S(O)CH2CH3.
30. The compound according to claim 19 or 27, wherein Q is optionally substituted in the ortho-position with J2, wherein J2 is C1-4alkyl, -C≡C-(C1-4alkyl), CH=CH2, CH=CHF, O(C1-4alkyl), NH(C1-4alkyl), N(C1-4alkyl)2, -(C1-4alkyl)OH, -(C1-4alkyl)O(C1-4alkyl),
-(C1-4alky I)NH2, -(C1-4alkyl)NH(C1-4alkyl), -(C1-4alkyl)N(C1-4alkyl)2, -(C!-4alkyl)CN, CO(C1-4alkyl), CON(C1-4alky I)2, C(O)O(C ,-4alkyl), S(C1-4alkyl), -S-(C1-4alkyl)NH2, S(O)(C1-4alkyl)NH2, S(O)2(C1-4alkyl)OH, S(O)(C1-4alkyl)NHC(O)O(t-butyl), NHS(O)2(C i.4alkyl), halo, or CN.
31. The compound according to claim 19 or claim 27, wherein J2 is CH2CH2OH, SCH(CH3)2", -C≡CCH3, halo, CN, CON(CH3)2, CH2CN, S(O)CH2CH2NH2, SCH2CH2NH2, C(O)OCH3, CH2N(CH3)2, S(O)CH2CH2NHBOC, N(CH3)2, NHSO2CH3, CH=CHF, CH2OCH3, CH=CH2, SCH2CH3, or -CH=CH.
32. The compound according to claim 19 or claim 27, wherein Q is optionally substituted in the para position with J2, wherein J2 is -SO2(C1-4alkyl), -SO2(C3.6cycloalkyl), -SO2(3-6 membered heterocyclyl), -SO2(C1-4alkyl)N(C1-4alkyl)2, -C(O)(C1-4aIkyl), -(C1-4alkyl)N(C1-4alkyl)2, or -NHC(O)(C1-4alkyl).
33. The compound according to claim 19 or claim 27, wherein Q is optionally substituted in the meta position with J2 wherein J2 is C1-4alkyl, C1-4alkoxy, halo, haloC1-4alkyl, haloC1- 4alkoxyl, CN, SO2(C1-4alkyl), NHSO2(C1-4alkyl), C(O)(C1-4alkyl), C(O)NH2, NHC(O)(C1- 4alkyl), -(C1-4alky I)-OH, -(C |.4alky I)-O(C |.4alkyl), -(C1-4alkyl)-NH2, -(C1-4alkyl)-N(C1- 4alkyl)2, or -(C1-4alkyl)NH(C1-4alkyl).
34. The compound according to claim 18, wherein Q is naphthyl or benzothienyl.
35. The compound according to claim 19, wherein Q is pyridyl.
36. The compound according to claim 19 or 35, wherein Q is substituted in the ortho- position with one J2, wherein J2 is CN.
37. The compound of claim 19 or 35, wherein Q is substituted with one or two J2, wherein J2 is a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O or NR".
38. The compound of claim 37, wherein J2 is -OCH3, -OCH2CH2N(CH3)2, -NHCH2CH2N(CH3)2, or piperazinyl.
39. The compound according to any one of claims 1-16, wherein p is 1.
40. The compound according to claim 39, wherein Q is phenyl, pyridyl, or naphthyl.
41. The compound according to claim 40, wherein Q is phenyl.
42. The compound according to claim 40, wherein said pyridyl is 3-pyridyl or 4-pyridyl.
43. The compound according to claim 15, wherein Q comprises Q1 and optionally Q2 as shown in formula IA-i, wherein Q1 is a six membered ring and -LNR1R2 is substituted in the para-position as shown below:
Figure imgf000376_0001
44. The compound of claim 15 and 43, wherein J5 is halogen, NO2, CN, O(haloC1-4aliphatic), haloC1-4aliphatic, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'.
45. The compound according to any one of claims 39-43, wherein L is -C(O)- or -SO2-.
46. The compound according to any one of claims 39-45, wherein R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1- 2 heteroatoms selected from nitrogen, sulfur, or oxygen.
47. The compound according to claim 46, wherein said heterocyclic ring is selected from pyrrolidinyl, piperidinyl, piperazinyl, morpholinyl, 1,4-diazepanyl, or 1 ,4-oxazepanyl.
48. The compound of claim 47, wherein said heterocyclic ring is optionally substituted with halo, CN, C1-6aliphatic, haloC1-6aliphatic, -C(O)O(C i.6aliphatic), C(O)H, C(O)(C |.6aliphatic), P(O)(OC1-4alkyl)2, NH(d.6aliphatic), or N(C1-6aliphatic)2.
49. The compound according to claim 43, wherein R1 is C1-C6alkyl.
50. The compound according to claim 43 or 49, wherein R2 is C1-Cήalkyl.
51. The compound according to claim 43 or 49, wherein R2 is -(C2-C6alkyl)-Z.
52. The compound of claim 11 or claim 15, wherein m is 0 and R5 is thienyl, thiazolyl, furanyl, pyrrolidinyl, azetidinyl, piperidinyl, piperazinyl, morpholinyl, pyridinonyl, pyridyl, tetrahydropyridinyl, tetrahydroisoquinolinyl, 1 ,4-diazepanyl, azabicyclo[2.2.1]heptanyl, or phenyl.
53. The compound of claim 52, wherein R5 is phenyl, piperidinyl or thienyl.
54. The compound of claim 53, wherein Q is optionally substituted in the para position with -SO2(C1-4alkyl), -SO2(C1-4alkyl)N(C1-4alkyl)2, C(O)N(C1-4alkyl)2, C(O)(1 , 4-diazepanyl), C(O)(piperazinyl), or C(O)(piperidinyl).
55. The compound of claim 52, wherein R5 is phenyl.
56. The compound of any one of claims 52-55, wherein R5 is optionally substituted with 1 -2 J5 groups, wherein J5 is selected from halo, CN, NO2, X'-R, or -(X\-Q4; p is 0-1 ; X1 is a C1-10aliphatic wherein 1-3 methylene units of said C1-6aliphatic are optionally replaced with -NR'-, -0-, -S-, C(=NH)-, C(O), S(O)2, or S(O); R is H; and Q4 is a 3-6 membered monocyclic ring containing 0-2 heteroatoms selected from O or N, wherein X1 is optionally substituted with 1-3 occurrences of halo or CN.
57. The compound of claim 55 or 56, wherein J5 is a C1-10aliphatic chain wherein 1-2 methylene units of X1 are replaced with -O- or -NR'-.
58. The compound of claim 53 or 54, wherein R5 is piperidinyl optionally substituted with NH2 or -(C1-4alkyl)NH2.
59. The compound of claim 52, wherein R5 is thienyl optionally substituted with CN, C1-6alkyl, -(C ,-4alky I)NH2, -<C1-4alkyl)NH(C1-6alkyl), -(C1-4alkyl)N(C1-6alkyl)2, 0(C1-6alkyl), pyrrolidinyl, wherein said alkyl is optionally substituted with 1-3 halo.
60. The compound of claim 53, wherein Q4 is an optionally substituted 3-6 membered cycloalkyl ring.
61. The compound of claim 53, wherein Q4 is an optionally substituted 3-6 membered heterocyclic ring selected from pyrrolidinyl, azetidinyl, or thienyl.
62. The compound of claim 53, wherein J5 is halo, C1-6alkyl, NO2, CN, d.6alkyl, -CH=CH2, OH, OCH3, OCH2CH3, OCH(CH3)2, NH2, CH2NH2, CH2OH, CH(CH3)NHCH3, C(CH3)2NH2, CH2CH2NH2, CH2CH2OH, CH2NHCH3, CH2N(CH3)2, CH(CH3)NH2, CH(CH3)NHC(O)O(CH3)3, CH2NHC(CH3)2, CH2NHCH2CHF2, CH2NHCH2CH(CH3)OH, CH2NHCH2C(CH3)2OH, CH2NHCH2CH(OH)-cyclopropyl, CH2NHCH2CH2N(CH3 )2, CH2NHCH(CH2CH3)3, CH2NHCH3, CH2NHCH2CH3, CH2NHCH2CH2CH3, CH2NH- cyclopropyl, CH2NHCH2CH2OH, CH2NHCH2CH2OCH3, CH2NHCH2CH2OCH2CH2OH, azetidinyl, pyrrolidinyl, CF3, C(=NH)NH2, C(=NH)NH(CH3), thienyl, CH2NH-cyclopropyl, CH2NH(CH2OH)3, OCH2CH2OH, OCH2CH2CH2OH, OCH2CH2NHC(O)OC(CH3)3, CH2NHC(O)O(CH3)3,or CH2OC(O)CH3.
63. The compound of claim 1 1 , 15, or 43, wherein m is 1.
64. The compound of claim 63, wherein R5 is H.
65. The compound of claim 63, wherein Y is -NH-, -NHCH2-, -NHC(O)-, C(O)NH, C(O)NHCH2, C(O), -NHCH(CH3)- or -N(CH3)CH2-; and R5 is phenyl.
66. The compound of claim 65 wherein R5 is optionally substituted with halo, C1-4alkyl wherein up .to 1 methylene unit is optionally replaced with O, NR', or S.
67. A compound of Formula IA-ii:
Figure imgf000378_0001
IA-ii or a pharmaceutically acceptable salt thereof; wherein Y is NH;
Ring A is a 5 membered heteroaryl ring selected from
Figure imgf000378_0002
Figure imgf000378_0003
R5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R5 is optionally substituted with 1-5 J5 groups;
L is -C(O)- or -SO2-;
R1 is H, or C1-C6alkyl;
R2 is -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of
Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1;
Jzι is -(X)1-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, r and m is independently 0 or 1 ;
Z is -NR3R4;
R3 is H or C)-C2alkyl;
R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; each Jz and J5 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic),
CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; J2 is halo, C1-C2alkyl optionally substituted with 1-3 fluoro or CN; q is O, 1, or 2.
68. The compound of claim 67, wherein Ring A is
Figure imgf000379_0002
, or
Figure imgf000379_0003
.
69. The compound of claim 67 or 68, wherein m is O.
70. The compound of claim 67 or 68, wherein q is 0.
71. The compound of claim any one of claims 67-70, wherein L is -C(O)-.
72. The compound of claim 71 , wherein R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms.
73. The compound of claim 71 or 72, wherein t is 1.
74. The compound of claim 71 or 72, wherein t is 0.
75. The compound of claim 73 or 74, wherein R1 and R2, taken together with the atom to which they are bound, form a heterocyclyl selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl.
76. The compound of claim 75, wherein said heterocyclyl is
Figure imgf000379_0001
77. The compound of claim 75, wherein JZI is (X)r-Z.
78. The compound of claim 75, wherein Jzι is C1-4alkyl or N(C1-4alkyl)2.
79. The compound of claim 68, wherein R1 is H or C|-C6alkyl; and R2 is -(C2-C6alkyl)-Z.
80. The compound of claim 79, wherein R3 and R4 are both C1-C2alkyl.
81. The compound of claim 73 or 74, wherein RJ and R", taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl.
82. The compound of claim 81, wherein said ring is pyrrolidinyl or piperidinyl.
83. The compound of claim 67 or claim 68, wherein m is 0, q is 0, and -L-NR1R2 is C(0)pyrrolidinyl, C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O) 1,4-diazepanyl, C(0)NH-piperidinyl, C(0)NHCH2CH2-pyrrolidinyl, C(0)NHCH2CH2-piperidinyl, CON(CHS)CH2CH2N(CHS)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl is optionally substituted with C1-4alkyl or N(C1-4alkyl)2.
84. The compound of any one of claims 1-3, having Formula IA-iii:
Figure imgf000380_0001
P IA-iii: wherein
Ring A is
Figure imgf000380_0002
or ,
Figure imgf000380_0003
J5O is H, F, Cl, C1-4aliphatic, O(C1-3aliphatic), or OH;
J5P is
Figure imgf000380_0004
J5pl is H, C1-4aliphatic, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl; wherein J5pl is optionally substituted with 1-2 occurrences of OH or halo;
J5p2 is H, methyl, ethyl, CH2F, CF3, or CH2OH;
J2O is H, CN, or SO2CH3;
J2m is H, F, Cl, or methyl;
J2P is -SO2(C1-6alkyl), -SO2(C3.6cycloalkyl), -SO2(4-6 membered heterocyclyl),
-SO2(C1-4alkyl)N(C1-4alkyl)2, or -SO2(C1-4alkyl)-(4-6 membered heterocyclyl), wherein said heterocyclyl contains 1 heteroatom selected from oxygen, nitrogen, or sulfur; and wherein said J2p is optionally substituted with 1-3 occurences halo, OH, or O(C1-4alkyl).
85. The compound of claim 84, wherein Ring A is .
Figure imgf000381_0002
86. The compound of claim 84, wherein Ring A is
Figure imgf000381_0003
87. A compound selected from the following:
Table IA-I
Figure imgf000381_0001
IA-I IA-2
Table IA-2
Figure imgf000381_0004
Figure imgf000382_0001
Figure imgf000383_0001
Figure imgf000384_0001
Figure imgf000385_0001
Figure imgf000386_0001
Figure imgf000387_0001
A compound selected from the following:
Table IA-3
Figure imgf000388_0001
Figure imgf000389_0001
Figure imgf000390_0001
Figure imgf000391_0001
Figure imgf000392_0001
Figure imgf000393_0001
Figure imgf000394_0001
Figure imgf000395_0001
Figure imgf000396_0001
Figure imgf000397_0001
Figure imgf000398_0001
Figure imgf000399_0001
Figure imgf000400_0001
Figure imgf000401_0001
Figure imgf000402_0001
Figure imgf000403_0001
Figure imgf000404_0001
Figure imgf000405_0001
Figure imgf000406_0001
Figure imgf000407_0001
Figure imgf000408_0001
Figure imgf000409_0001
Figure imgf000410_0001
Figure imgf000411_0001
Figure imgf000412_0001
Figure imgf000413_0001
Figure imgf000414_0001
Figure imgf000415_0001
Figure imgf000416_0001
Figure imgf000417_0001
Figure imgf000418_0002
89. A compound selected from the following:
Table IVA
Figure imgf000418_0003
90. A compound selected from the following:
Table IA-4
Figure imgf000418_0001
Figure imgf000419_0001
Figure imgf000420_0001
P31 P33
P34 P36
P37 P39
Figure imgf000421_0001
Figure imgf000422_0001
Figure imgf000423_0001
Figure imgf000424_0001
Figure imgf000425_0001
Figure imgf000426_0001
Figure imgf000427_0001
Figure imgf000428_0001
Figure imgf000429_0001
Figure imgf000430_0001
91. A compound of Formula IIIA:
Figure imgf000430_0002
1HA or a pharmaceutically acceptable salt thereof; wherein
Y is a C1-C4alkyl chain wherein one methylene unit of the alkyl chain is optionally replaced with -NR0-; Q is phenyl or pyridyl;
R5 is 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, R5 is optionally fused to a 5-6 membered aromatic ring containing 0-2 heteroatoms selected from N, O, or S; each R5 is optionally substituted with 1-5 J5 groups; L is-C(O)- or -SO2-; R1 is H, or C1-C6alkyl; R0 is H or C1-C6alkyl; R2 is C1-C6alkyl, -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from nitrogen, sulfur, or oxygen; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, r and m is independently 0 or 1 ; Z is -NR3R4; R3 is H or C|-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said ring is optionally substituted with one occurrence of Jz; Jz is NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C 1-4aliphatic),
NO2, CN, CO2H, CO(C1-4aliphatic), CO2(C1-4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; J5 is halogen, NO2, CN, 0(haloC1-4aliphatic), haloC1-4aliphatic, or a C1 -6aliphatic group wherein up to 2 methylene units are optionally replaced with C(O), O, or NR'; J2 is halo; CN; phenyl; oxazolyl; or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said d.6aliphatic group is optionally substituted with 1 -3 fluoro or CN; R' and R" are each independently H or Cj-C4 alkyl; q is O, I , or 2, p is O or 1.
92. The compound of claim 91, wherein p is 0.
93. The compound of claim 91 or claim 92, wherein Q is pyridyl.
94. The compound of any one of claims 91-93, wherein m is 0.
95. The compound of any one of claims 91-94, wherein R is phenyl or thienyl.
96. The compound of claim 94 or 95, wherein R5 is phenyl optionally substituted with one occurrence OfNH2, C1-C4alkyl, or CH2NH2.
97. The compound of claim 91, selected from the following:
Figure imgf000432_0002
Figure imgf000432_0001
98. A compound of formula II,
Figure imgf000433_0001
or a pharmaceutically acceptable salt thereof; wherein
Ring A is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur;
L is -C(O)-;
R1 is C1-C6alkyl;
R2 is -(C2-C6alkyl)-Z or a 4-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of
Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)r-CN, C1-C6alkyl or -(X)r-Z; X is C1-C4alkyl; each t, p, and r is independently 0 or 1 ; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-Cealkyl; or R3 and R , taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1 -2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; each J^ and J1 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C 1-4aliphatic), CO2(C i -4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic;
J2 is halo, C|-C2alkyl optionally substituted with 1-3 fluoro, or CN; q is 0, 1 , or 2;
provided that when Ring A is
Figure imgf000434_0002
, and R1 is H; then R2 is not
Figure imgf000434_0003
or
Figure imgf000434_0004
99. The compound of claim 98, wherein Ring A is a 6-membered ring not fused to another ring. l OO.The compound of claim 99, wherein Ring A is phenyl, pyridyl, or pyrimidyl.
101. The compound of claim 100, wherein Ring A is phenyl.
102. The compound of any one of claims 98-101 , wherein R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms.
103. The compound of claim 102, wherein t is 1.
104. The compound of claim 102, wherein t is 0.
105. The compound of claim 103 or claim 104, wherein the heterocyclic ring formed by R1 and R2 is pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl. lOό.The compound of claim 105, wherein the heterocyclic ring formed by R and R is
Figure imgf000434_0001
107. The compound of claim 106, wherein the ring formed by R1 and R2 is optionally substituted with CH2pyrrolidinyl, C 1-4alkyl, N(C1-4alkyl)2, or CH2CH2CN.
108. The compound of any one of claims 98 - 101, wherein R2 is -(C2-C6alkyl)-Z.
109.The compound of claim 107 or 108, wherein R3 and R4 are both C1-C2alkyl.
1 10. The compound of claim 107 or 108, wherein R3 and R4, taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl.
11 l .The compound of claim 1 10, wherein said ring is pyrrolidinyl or piperidinyl.
1 12. The compound of claim 98, wherein p is 0, q is 0, and -L-NR1R2 is C(0)pyrrolidinyl,
C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O)1, 4-diazepanyl, CON(CH3)CH2CH2N(CH3)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl is optionally substituted with CH2pyrrolidinyl, C1-4alkyl, N(C1-4alkyl)2, or CH2CH2CN.
1 13.A compound selected from the following:
Figure imgf000435_0001
Figure imgf000436_0001
11-10 IM l .
1 14. A pharmaceutical composition comprising a compound of any one of claims 98-1 13 and a pharmaceutically acceptable carrier.
115. A compound of formula III:
Figure imgf000436_0002
or a pharmaceutically acceptable salt thereof; wherein
L is -C(O)- or -SO2-;
R1 is H, or C1-C6alkyl;
R2 is -(C2-C6alkyl)-Z or a 4-8 membered heterocyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is , optionally substituted with one occurrence of JZ1; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, p, and r is independently 0 or 1 ;
Z is -NR3R4;
R3 is H or C1-C2alkyl;
R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1 -2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of J ; each Jz and J1 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen,
C1 -4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic),
Cθ2(C1-4aliphatic), O(haloC1 -4aliphatic), or haloC1-4aliphatic; J2 is halo, C)-C2alkyl optionally substituted with 1-3 fluoro, or CN; q is O, 1, or 2; provided that the following compounds of formula III-i are excluded:
Figure imgf000437_0001
wherein
Jla is H;
Jlb is H or F;
R1 is H; and R2 is -(C1-4alkyl)-N(CH3)2; or
R , 1 a -_ndJ r R>2 taken together are
Figure imgf000437_0002
or
Figure imgf000437_0003
1 16. The compound of claim 1 15, wherein L is -C(O)-.
1 17. The compound of claim 1 15 or 1 16, wherein R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms.
1 18. The compound of any one of claims 1 15-1 17, wherein t is 1.
1 19. The compound of any one of claims 1 15-1 17 wherein t is O.
120. The compound of claim 118 or claim 119, wherein the heterocyclic ring formed by R1 and R is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl. 121. The compound of claim 120, wherein said heterocyclyl is
Figure imgf000438_0001
122. The compound of claim 120 or claim 121, wherein J τz is Chalky 1 or N(C1-4alkyl)2. 123. The compound of claim 1 16, wherein R1 is H or C1-C6alkyl; and R2 is -(C2-C6alkyl)-Z. 124. The compound of any one of claims 1 15-123, wherein R and R4 are both C|-C2alkyl. 125. The compound of any one of claims 1 15-123, wherein R3 and R4, taken together with the atom to which they are bound, form a ring selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1 ,4-diazepanyl.
126. The compound of claim 125, wherein said ring is pyrrolidinyl or piperidinyl. 127. The compound of claim 1 15, wherein p is 0, q is 0, and -L-NR1R2 is C(O)pyrrolidinyl, C(O)piperidinyl, C(O)piperazinyl, C(O)azepanyl, C(O) 1,4-diazepanyl, C(O)NH-piperidinyl, C(O)NHCH2CH2-pyrrolidinyl, C(O)NHCH2CH2-piperidinyl, CON(CH3)CH2CH2N(CHS)2, wherein said pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl is optionally substituted with C1-4alkyl or N(C1-4alkyl)2. 128. A compound selected from the following:
Figure imgf000438_0002
Figure imgf000438_0003
Figure imgf000439_0001
129.A compound selected from the following:
Figure imgf000439_0002
Figure imgf000440_0001
Figure imgf000441_0001
Figure imgf000442_0001
130. A compound of Formula V:
Figure imgf000443_0001
V or a pharmaceutically acceptable salt thereof: wherein Ring A is a 8-9 membered bicyclic heteroaryl ring having 1-4 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur; Q is a 5-6 membered monocyclic aromatic ring containingO-3 heteroatoms independently selected from the group consisting of nitrogen, oxygen, and sulfur; L is C1-4alkyl chain wherein up to two methylene units of the alkyl chain are optionally replaced with O, NR6, S, -C(O)-, -SO-, or -SO2-; R1 is H or C1-C6alkyl; R2 is H, C1-C6alkyl, -(C2-C6alkyl)-Z, or a 3-8 membered cyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 3-8 membered monocyclic or 8-9 membered bicyclic heterocyclic ring containing 1-2 heteroatoms selected the group consisting of oxygen, nitrogen, and sulfur; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)1-Z1; X is C1-4alkyl; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; Z1 is -NR5R6; R5 is H or C1-C2alkyl; R° is H or C1-C6alkyl; or R5 and R6, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jzι; J is halo, CN, or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said C1-6aliphatic group is optionally substituted with 1-3 fluoro or CN; J2 is halo; CN; or a C1-6aliphatic group wherein up to 2 methylene units are optionally replaced with O, NR", C(O), S, S(O), or S(O)2; said d.6aliphatic group is optionally substituted with 1-3 fluoro or CN; each Jz and JZ1 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic),
CO2(C i.4aliphatic), O(haloC1-4aliphatic), or haloC1-4aliphatic; each q and m is independently O, 1 , or 2; each t, p, and r is independently O or 1.
131.The compound of claim 130, wherein Ring A is a 9-membered ring.
132. The compound of claim 130, wherein Ring A is a 5-6 bicyclic ring system.
133. The compound of any one of claims 130-132, wherein Ring A has 1-2 heteroatoms.
134. The compound of claim 133, wherein Ring A is benzimidazolyl, benzoxazolyl, indazolyl, benzothiazolyl, indolyl, benzotriazolyl, or azaindolyl.
135. The compound of any one of claims 130-134, wherein Ring Q is phenyl or pyridyl.
136. The compound of claim 135, wherein Q is phenyl.
137. The compound of claim 136, wherein p is 1 and Ring Q is substituted in the para position with L-NR1R2 as shown in formula I-a:
Figure imgf000444_0001
I-a 138. The compound of claim 137, wherein L is C(O) or S(O)2.
139. The compound of claim 137 or 138, wherein R' and R"" are both C1-4alkyl.
140. The compound of claim 137 or 138, wherein R1 and R2, taken together with the atom to which they are bound, form a 4-7 membered heterocyclic ring containing 1-2 nitrogen atoms.
141. The compound of claim 140, wherein said heterocyclic ring is selected from the group consisting of pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, and 1,4-diazepanyl.
142. The compound of claim 141, wherein said heterocyclyl is 1,4-diazepanyl.
143. The compound of claim 135, wherein Q is pyridyl.
144. The compound of claim 143, wherein p is 0.
145. The compound of claim 143 or 144, wherein m is 1 and J2 is CN.
146. A compound selected from the following:
Figure imgf000445_0001
Figure imgf000446_0003
147. A compound selected from one of the following compounds:
Figure imgf000446_0001
Figure imgf000446_0002
Figure imgf000447_0001
Figure imgf000448_0001
148.A compound of formula VI:
Figure imgf000449_0001
VI or a pharmaceutically acceptable salt thereof; wherein Q is a 5-6 membered monocyclic aromatic ring containing 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; or an 8-10 membered bicyclic aromatic ring containing 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; L is -C(O)- or -SO2-; G is S or O; R1 is H, or C1-C6alkyl; R2 is -(C2-C6alkyl)-Z or a 4-8 membered heterocyclic ring containing 0-2 nitrogen atoms; wherein said ring is bonded via a carbon atom and is optionally substituted with one occurrence of Jz; or R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said heterocyclic ring is optionally substituted with one occurrence of JZ1; JZ1 is -(X)1-CN, C1-C6alkyl or -(X)1-Z; X is C1-C4alkyl; each t, p, and r is independently 0 or 1 ; Z is -NR3R4; R3 is H or C1-C2alkyl; R4 is H or C1-C6alkyl; or R3 and R4, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1-2 nitrogen atoms; wherein said ring is optionally substituted with one occurrence of Jz; each Jz and J1 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO(C1-4aliphatic),
CO2(C1-4aliphatic), 0(haloC1-4aliphatic), or haloC1-4aliphatic; J2 is halo, C1-C2alkyl optionally substituted with 1-3 fluoro, or CN; q is O, I , or 2; p is O or 1.
149. The compound of claim 148, wherein p is 1.
150. The compound of claim 148 or 149, wherein Q is phenyl.
151. The compound of any one of claims 148-150, wherein L is -C(O)-.
152. The compound of any one of claims 148-151 , wherein R1 and R2, taken together with the atom to which they are bound, form a 4-8 membered heterocyclic ring containing 1 -2 nitrogen atoms.
153. The compound of claim 152, wherein the heterocyclic ring formed by R1 and R2 is selected from pyrrolidinyl, piperidinyl, piperazinyl, azepanyl, or 1,4-diazepanyl. 154. The compound of claim 153, wherein said heterocyclyl is
Figure imgf000450_0001
155. The compound of claim 148, wherein p is 0, q is 0, and -L-NR1R2 is C(O)1 , 4-diazepanyl.
156.A compound selected from the following:
Figure imgf000450_0002
Figure imgf000450_0003
157. A compound of formula VII,
Figure imgf000451_0001
or a pharmaceutically acceptable salt thereof; wherein
Ring A is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said ring is optionally substituted with J1; R1 is C-C6alkyl; J1 is a C1-6 alkyl chain wherein 1-2 methylene units are optionally replaced with O, NR*, S, or C(O); J1 is optionally substituted with 1-3 occurrences of halo;
R* is H or C1-4alkyl; JJ22 iiss hhaalloo,, C C1i--CC22aallkkyyll optionally substituted with 1-3 fluoro, or CN; Each p and q is independently 0, 1 , or 2.
158. The compound of claim 157, wherein Ring A is a 5-6 membered heteroaryl having 1-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur.
159. The compound of claim 158, wherein Ring A is pyridinyl, pyrimidyl, pyrazinyl, triazinyl, pyrrolyl, pyrazolyl, triazolyl, thienyl, thiazolyl, thiadiazolyl, furanyl, oxazolyl, or oxadiaozolyl. lόO.The compound of claim 161 , wherein Ring A is pyridinyl, pyrazolyl, thiadiazolyl, or thiazolyl wherein Ring A is optionally substituted with halo or C1-4alkyl.
161. The compound of claim 157, wherein Ring A is phenyl.
162. The compound of any one of claims 157-161, wherein said phenyl is substituted with one occurrence of J1.
163. The compound of claim 162, wherein J1 is a C\.β alkyl chain wherein 1 methylene unit is replaced with N or O.
164. The compound of claim 163, wherein J1 is O(C1-4alkyl) or -(C1-4alkyl)NH(C1-4alkyl).
165. The compound of claim 164, wherein J1 is -<C1-4alkyl)NH(C1 -4alkyl).
166.A compound selected from the following:
Figure imgf000452_0001
Figure imgf000453_0001
Figure imgf000454_0002
167. A compound selected from the following:
Figure imgf000454_0001
Figure imgf000455_0001
Figure imgf000456_0001
1-
1-
Figure imgf000457_0001
Figure imgf000458_0001
Figure imgf000459_0001
Figure imgf000460_0001
Figure imgf000461_0001
Figure imgf000462_0001
Figure imgf000463_0001
Figure imgf000464_0001
168. A pharmaceutical composition comprising a compound of any one of claims 1-167 and a pharmaceutically acceptable carrier.
169. A method for treating cancer in a patient comprising administering a compound of any one of claims 1-166 or a pharmaceutically acceptable derivative thereof.
170. The method of claim 169, further comprising administering to said patient an additional therapeutic agent selected from a DNA-damaging agent; wherein said additional therapeutic agent is appropriate for the disease being treated; and said additional therapeutic agent is administered together with said compound as a single dosage form or separately from said compound as part of a multiple dosage form.
171. The method of claim 170, wherein said DNA-damaging agent is selected chemotherapy or radiation treatment.
172. The method of claim 170, wherein said DNA-damaging agent is selected from ionizing radiation, radiomimetic neocarzinostatin, a platinating agent, a Topo I inhibitor, a Topo II inhibitor, an antimetabolite, an alkylating agent, an alkyl sulphonates, an antimetabolite, or an antibiotic.
173. The method of claim 172, wherein said DNA-damaging agent is selected from ionizing radiation, a platinating agent, a Topo I inhibitor, a Topo II inhibitor, or an antibiotic.
174. The method of claim 173, wherein said platinating agent is selected from Cisplatin, Oxaliplatin, Carboplatin, Nedaplatin, Lobaplatin, Triplatin Tetranitrate, Picoplatin, Satraplatin, ProLindac and Aroplatin; said Topo I inhibitor is selected from Camptothecin, Topotecan, Irinotecan/SN38, Rubitecan and Belotecan; said Topo II inhibitor is selected from Etoposide, Daunorubicin, Doxorubicin, Aclarubicin, Epirubicin, Idarubicin, Amrubicin, Pirarubicin, Valrubicin, Zorubicin and Teniposide; said antimetabolite is selected from Aminopterin, Methotrexate, Pemetrexed, Raltitrexed, Pentostatin, Cladribine, Clofarabine, Fludarabine, Thioguanine, Mercaptopurine, Fluorouracil, Capecitabine, Tegafur, Carmofur, Floxuridine, Cytarabine, Gemcitabine, Azacitidine and Hydroxyurea; said alkylating agent is selected from Mechlorethamine, Cyclophosphamide, Ifosfamide, Trofosfamide, Chlorambucil, Melphalan, Prednimustine, Bendamustine, Uramustine, Estramustine, Carmustine, Lomustine, Semustine, Fotemustine, Nimustine, Ranimustine, Streptozocin, Busulfan, Mannosulfan, Treosulfan, Carboquone, ThioTEPA, Triaziquone, Triethylenemelamine, Procarbazine, Dacarbazine, Temozolomide, Altretamine, Mitobronitol, Actinomycin, Bleomycin, Mitomycin and Plicamycin.
175. The method of claim 174, wherein said platinating agent is selected from Cisplatin, Oxaliplatin, Carboplatin, Nedaplatin, or Satraplatin; said Topo I inhibitor is selected from Camptothecin, Topotecan, irinotecan/SN38, rubitecan; said Topo II inhibitor is selected from Etoposide; said antimetabolite is selected from methotrexate, pemetrexed, Thioguanine, Fludarabine, Cladribine, Cytarabine, gemcitabine, 6-Mercaptopurine, or 5-Fluorouracil; said alkylating agent is selected from nitrogen mustards, nitrosoureas, triazenes, alkyl sulfonates, Procarbazine, or aziridines; and said antibiotic is selected from Hydroxyurea, Anthracyclines, Anthracenediones, or Streptomyces family.
176. The method of claim 173 wherein said DNA-damaging agent is a platinating agent or ionizing radiation.
177. The method of any one of claims 169-176, wherein said cancer is a solid tumor selected from the following cancers: Oral: buccal cavity, lip, tongue, mouth, pharynx; Cardiac: sarcoma (angiosarcoma, fibrosarcoma, rhabdomyosarcoma, liposarcoma), myxoma, rhabdomyoma, fibroma, lipoma and teratoma; Lung: bronchogenic carcinoma (squamous cell or epidermoid, undifferentiated small cell, undifferentiated large cell, adenocarcinoma), alveolar (bronchiolar) carcinoma, bronchial adenoma, sarcoma, lymphoma, chondromatous hamartoma, mesothelioma; Gastrointestinal: esophagus (squamous cell carcinoma, larynx, adenocarcinoma, leiomyosarcoma, lymphoma), stomach (carcinoma, lymphoma, leiomyosarcoma), pancreas (ductal adenocarcinoma, insulinoma, glucagonoma, gastrinoma, carcinoid tumors, vipoma), small bowel or small intestines (adenocarcinoma, lymphoma, carcinoid tumors, Karposi's sarcoma, leiomyoma, hemangioma, lipoma, neurofibroma, fibroma), large bowel or large intestines (adenocarcinoma, tubular adenoma, villous adenoma, hamartoma, leiomyoma), colon, colon-rectum, colorectal; rectum, Genitourinary tract: kidney (adenocarcinoma, Wilm's tumor [nephroblastoma], lymphoma), bladder and urethra (squamous cell carcinoma, transitional cell carcinoma, adenocarcinoma), prostate (adenocarcinoma, sarcoma), testis (seminoma, teratoma, embryonal carcinoma, teratocarcinoma, choriocarcinoma, sarcoma, interstitial cell carcinoma, fibroma, fibroadenoma, adenomatoid tumors, lipoma); Liver: hepatoma (hepatocellular carcinoma), cholangiocarcinoma, hepatoblastoma, angiosarcoma, hepatocellular adenoma, hemangioma, biliary passages; Bone: osteogenic sarcoma (osteosarcoma), fibrosarcoma, malignant fibrous histiocytoma, chondrosarcoma, Ewing's sarcoma, malignant lymphoma (reticulum cell sarcoma), multiple myeloma, malignant giant cell tumor chordoma, osteochronfroma (osteocartilaginous exostoses), benign chondroma, chondroblastoma, chondromyxofibroma, osteoid osteoma and giant cell tumors; Nervous system: skull (osteoma, hemangioma, granuloma, xanthoma, osteitis deformans), meninges (meningioma, meningiosarcoma, gliomatosis), brain (astrocytoma, medulloblastoma, glioma, ependymoma, germinoma [pinealoma], glioblastoma multiform, oligodendroglioma, schwannoma, retinoblastoma, congenital tumors), spinal cord neurofibroma, meningioma, glioma, sarcoma); Gynecological: uterus (endometrial carcinoma), cervix (cervical carcinoma, pre-tumor cervical dysplasia), ovaries (ovarian carcinoma [serous cystadenocarcinoma, mucinous cystadenocarcinoma, unclassified carcinoma], granulosa-thecal cell tumors, Sertoli-Leydig cell tumors, dysgerminoma, malignant teratoma), vulva (squamous cell carcinoma, intraepithelial carcinoma, adenocarcinoma, fibrosarcoma, melanoma), vagina (clear cell carcinoma, squamous cell carcinoma, botryoid sarcoma (embryonal rhabdomyosarcoma), fallopian tubes (carcinoma), breast; Skin: malignant melanoma, basal cell carcinoma, squamous cell carcinoma, Karposi's sarcoma, keratoacanthoma, moles dysplastic nevi, lipoma, angioma, dermatofibroma, keloids, psoriasis, Thyroid gland: papillary thyroid carcinoma, follicular thyroid carcinoma; medullary thyroid carcinoma, multiple endocrine neoplasia type 2A, multiple endocrine neoplasia type 2B, familial medullary thyroid cancer, pheochromocytoma, paraganglioma; and Adrenal glands: neuroblastoma.
178. The method of claim 177, wherein said cancer is selected from lung cancer, head and neck cancer, pancreatic cancer, gastric cancer, and brain cancer.
179.A method of promoting cell death in cancer cells comprising administering to a patient a compound of any one of claims 1-166.
180. A method of preventing cell repair from DNA damage comprising administering to a patient a compound of any one of claims 1-166.
181. A method of inhibiting ATR in a biological sample comprising the step of contacting a compound of any one of claims 1-166 with said biological sample.
182. The method of claim 181, wherein said biological sample is a cell.
183. A method of sensitizing cells to DNA damaging agents comprising administering to a patient a compound of any one of claims 1-166.
184. The method of any one of claims 169-183, wherein said cell is a cancer cell having defects in the ATM signaling cascade.
185. The method of claim 184, wherein said defect is altered expression or activity of one or more of the following: ATM, p53, CHK2, MREl 1, RAD50, NBSl , 53BP1 , MDCl or H2AX.
186. The method of any one of claims 169-183, wherein said cell is a cancer cell expressing DNA damaging oncogenes.
187. The method of claim 186, wherein said cancer cell has altered expression or activity of one or more of the following: K-Ras, N-Ras, H-Ras, Raf, Myc, Mos, E2F, Cdc25A, CDC4, CDK2, Cyclin E, Cyclin A and Rb.
188. Use of a compound of any one of claims 1-166 as a radio-sensitizer or a chemo- sensitizer.
189. Use of a compound according to any one of claims 1-166 as a single agent (monotherapy) for treating cancer.
190. Use of according to any one of claims 1-166 or treating patients having cancer with a DNA-damage response (DDR) defect.
191.The use according to claim 190, wherein said defect is a mutation or loss of ATM, p53, CHK2, MREI l, RAD50, NBSl, 53BP1 , MDCl, or H2AX.
192. A method for inhibiting ATR in a patient comprising administering a compound of formula I:
Figure imgf000468_0001
wherein R1 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R1 is optionally substituted with 1-5 J1 groups; R2 is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R2 is optionally substituted with 1-5 J2 groups; L is -C(O)NH- or -C(O)N(C i-6alkyl)-; n is 0 or 1 ;
Each J1 and J2 is independently halo, -CN, -NO2, -V'-R, or -(V2)m-Q; V1 is a C1-10aliphatic chain wherein 0-3 methylene units are optionally and independently replaced with O, NR", S, C(O), S(O), or S(O)2; V1 is optionally substituted with 1 -6 occurrences of JV1; V2 is a C1-10aliphatic chain wherein 0-3 methylene units are optionally and independently replaced with O, NR", S, C(O), S(O), or S(O)2; V2 is optionally substituted with 1 -6 occurrences of JV2; m is 0 or 1 ;
Q is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 9-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q is optionally substituted with 0-5 JQ; each JV1 or JV2 is independently halogen, CN, NH2, NO2, C1 -4aI iphatic, NH(C1-4aliphatic), N(C1 -4 aliphatic)2, OH, O(C1-4aliphatic), CO2H, CO2(C1-4aliphatic), C(O)NH2, C(O)NH(C i .4al iphatic), C(O)N(C i -4aliphatic)2, NHCO(C , -4al iphatic), N(C1-4aliphatic)CO(C1-4aliphatic), SO2(C1-4aliphatic), NHSO2(C1-4al iphatic), or N(C1-4aliphatic)SO2(C1-4aliphatic), wherein said C1-4aliphatic is optionally substituted with halo;
R is H or C1-6aliphatic wherein said C1-6aliphatic is optionally substituted with 1-4 occurrences OfNH2, NH(C1-4al iphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4al iphatic), NO2, CN, CO2H, CO2(C1-4aliphatic), CO(C1-4aliphatic), O(haloC1-4al iphatic), or haloC1-4aliphatic; each JQ is independently halo, oxo, CN, NO2, X-R, or -<X)P-Q4; p is O or 1 ;
X is C1 -10aliphatic; wherein 1-3 methylene units of said C1-6aliphatic are optionally replaced with -NR, -O-, -S-, C(O), S(O)2, or S(O); wherein X is optionally and independently substituted with 1-4 occurrences OfNH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO(C1-4al iphatic), CO2H, CO2(C ι.4al iphatic), C(O)NH2, C(O)NH(Cl-4aliphatic), C(O)N(C1-4aliphatic)2, SO(C1-4aliphatic), SO2(C1-4al iphatic), SO2NH(C1-4aliphatic), SO2N(C1-4aliphatic)2, NHC(O)(C1-4aliphatic), N(C1-4aliphatic)C(O)(C1-4aliphatic), wherein said C1-4aliphatic is optionally substituted with 1-3 occurrences of halo;
Q4 is a 3-8 membered saturated or unsaturated monocyclic ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 8-10 membered saturated or unsaturated bicyclic ring having 0-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each Q4 is optionally substituted with 1-5 JQ4;
J is halo, CN, or C1-4alkyl wherein up to 2 methylene units are optionally replaced with O, NR*, S, C(O), S(O), or S(O)2;
R is H or C1-4alkyl wherein said C1-4alkyl is optionally substituted with 1 -4 halo; R', R", and R* are each independently H, C1-4alkyl, or is absent; wherein said C1-4alkyl is optionally substituted with 1-4 halo.
193. The method of claim 192, wherein
R1 is a 5-6 membered monocyclic.aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R1 is optionally substituted with 1-5 J1 groups;
R is a 5-6 membered monocyclic aryl or heteroaryl ring having 0-3 heteroatoms independently selected from nitrogen, oxygen, or sulfur, wherein said monocyclic aryl or heteroaryl ring is optionally fused to another ring to form an 8-10 membered bicyclic aryl or heteroaryl ring having 0-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R2 is optionally substituted with 1-5 J2 groups; each JV1 or JV2 is independently NH2, NH(C1-4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO2(C i.4aliphatic), CO(C1-4aliphatic), 0(haloC1-4aliphatic), or haloC1-4aliphatic;
• each JQ is independently halogen, NO2, CN, or C1-6aliphatic wherein up to 1 methylene unit is optionally replaced with NR', O, S, CO, CO2, CONR', SO, SO2, SO2NR', OCO, NR'CO, NR'COO, NR'SO, NR5SO2, NR'SO2NR', OCONR', or NR'CONR'; wherein said C1- βaliphatic is optionally substituted with 1-4 substituents selected from NH2, NH(C1- 4aliphatic), N(C1-4aliphatic)2, halogen, C1-4aliphatic, OH, O(C1-4aliphatic), NO2, CN, CO2H, CO2 (C1-4aliphatic), C0(C1-4aliphatic), 0(haloC1-4aliphatic), or haloC1-4aliphatic.
194. The method of claim 192 or 193, wherein R2 is substituted with one or two occurrences ofJ2.
195. The method of claim 194, wherein R2 is substituted with one occurrence of J2.
196. The method of any one of claims 192-195 wherein J2 is -(V2)m-Q or V '-R; each V1 and V is independently a C1-6aliphatic chain wherein 0-3 methylene units are optionally replaced with O, NR', S, C(O), S(O), or S(O)2; wherein the first or second methylene group away from the point of attachment is replaced with C(O), S(O), or
S(O)2, S or O; m is 1 ; R is H; and Q is a 5-7 membered monocyclic ring containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur; wherein said Q is optionally substituted with 1-3 occurrences of halo, C1-3alkyl, CN, OH, O(C1-3alkyl), NH2, NH(C1-3alkyl), N(C1-3alkyl)2, or CO(C1-3alkyl).
197. The method of claim 196, wherein Q is a 5-6 membered ring.
198. The method of claim 196, wherein n is O and R1 is a 5-6 membered monocyclic aromatic ring having 1-4 heteroatoms independently selected from nitrogen, oxygen, or sulfur, or a 9- 10 membered bicyclic aromatic ring having 1-6 heteroatoms independently selected from nitrogen, oxygen, or sulfur; each R1 is optionally substituted with 1-5 J1 groups.
199. The method of claim 198, wherein R1 is benzothiazole, oxadiazole, benzoxazole triazole, thiadiazole, or isoxazole.
200. The method of claim 199, wherein J1 is phenyl optionally substituted with NH2, C1-4alkyl, thiophene, or CH2NH2.
201. The method of claim any one of claims 192-200, wherein R2 is phenyl or pyridyl.
202. The method of claim 201 , wherein J2 is SO2(C1-4alkyl).
203. The method of claim 198, wherein R1 is benzimidazole, benzothioazole, oxadiazole, isoxazole, or triazole.
204. The method of claim 203, wherein R1 is benzimidazole.
205. The method of claim 203 or 204, wherein J1 is halo, CN, NO2, or -V'-R.
2O6.The method of claim 205, wherein J1 is halo, CN, C1-6alkyl, OR, SR, NR"R, C(O)R,
C(O)OR, C(O)NR"R, S(O)2R, or S(O)R.
207. The method of claim 204, wherein R2 is a 5-6 membered monocyclic ring.
208. The method of claim 207, wherein R2 is a 6-membered ring. 209. The method of claim 208, wherein R2 is phenyl or pyridyl.
21O.The method of any one of claims 207-209, wherein J2 is -(V2)m-Q or -V'-R wherein V1 and V2 are O, NR", -CO-, or -SO2-; Q is a 5-6 membered heterocyclic ring containing 1-2 heteroatoms selected from N or O; and JV1 and JV2 are halo. 21 1. The method of claim 210, wherein J2 is SO2CH3, morpholinyl, CH2OH, C(0)-(morpholinyl), C(O)NH(C1-4alky I)OH, piperazinyl, CN, CH2NHC(O)CH3, halo, C(O)NH(C1-4alkyl)pyrrolidinyl, or S02(pyrrolidinyl).
212. The method of claim 198, wherein n is 0;
R1 is benzimidazole, benzothiazole, benzoxazole, oxadiazole, isoxazole, thiadiazole, or triazole;
J1 is halo, CN, NO2, or -V'-R;
R2 is phenyl or pyridyl; j2 is -(V2)m-Q or -V'-R; m is 1 ;
V1 and V2 are -SO2-, -O-, -NR-, or -CO -;
Q is a 5-6 membered heterocyclic ring containing 1 -2 heteroatoms selected from nitrogen or oxygen; and
R is H or C1-6alkyl wherein said C1-6alkyl is optionally substituted with 1-4 halo. 213. The method of claim 212, wherein R1 is benzimidazole, benzothiazole, oxadiazole, isoxazole, or triazole.
214. The method of claim 192 wherein n is 1.
215. The method of claim 214, wherein R1 is phenyl, pyridyl, pyrimidyl, pyrazinyl, piperonyl, indolyl, benzimidazolyl, indazolyl, benzothiazolyl, benzothiophenyl, benzoxazolyl, pyrrolopyrimidinyl, pyrrolopyridinyl, azaindazolyl, or azaindolyl.
216. The method of claim 215, wherein R1 is phenyl, pyridyl, pyrimidyl, piperonyl, or indole. 217. The method of claim 216, wherein R1 is phenyl.
218.The method of any one of claims 214-217, wherein J1 is C1-6alkyl, CN, halo, OR, NR"R, SR, COR, CO2R, CONR"R, SOR, SO2R, S(O)2NR"R, OCOR, NRC(O)R, NRCOOR, NRSUR, NRSO2R, NRSO2NR"R, 0C0NR"R, or NRCONR"R; 4-6 membered fully saturated monocyclic ring containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur.
219.The method of claim 218, wherein J1 is C1-6alkyl, CN, halo, OR, NH2, CONH2, S(O)2NH2, NC(O)R, or pyrrolidinyl.
220. The method of any one of claims 213-219, wherein R2 is phenyl, pyridyl, indole, furanyl, pyrazole, thiophene, tetrahydropyran, or indazole.
221. The method of claim 220, wherein J2 is halo, CN, NH2, C1-6alkyl, OR, SO2R, NHSO2R, COOR, CONH2, morpholinyl, -V'-R, or -(V2)m-Q, wherein
V1 and V2 are CO, -CONR-, -CONR-(C1-4alkyl)-, -CONR-(C1-4alkyl)-OCH2-,
-CONR-(C1-4alkyl)-N(CH3)-,
R is H or C1-4alkyl; and Q is 1,4-diazepanyl, azetidinyl optionally substituted with OMe, piperidinyl optionally substituted with C1-4alkyl, 4-CH2OH, CONH2, pyrrolidinyl, OH, or CH2-pyrrolidinyl; piperazinyl optionally substituted with CH2CH2CN, CH3, COCH3; pyrrolidinyl optionally substituted with dimethylamino, tetrahydropyran; or C3-iocyclolkyl optionally substituted with OH.
222. The method of claim 220 or claim 221 , wherein J2 is SO2CH3, NHSO2CH3, CN, OH, OCH3, F, N(CH3)2, NHSO2CH3, CF3, C1-6alkyl, C0( 1 ,4-diazepanyl), COOH, CONH2, CON(CH3)2, CO(azetidinyl), CON(CH3)(C1-4alky I)OCH3, CONH(C1-4alkyl)piperazinyl, CONH(C1-4alkyl)piperidinyl, CONH-tetrahydropyran, CON(methylpiperidinyl), )
CO(piperidinyl), CONH-cyclopropyl, CO(morpholinyl), CON(CH3)-(C1-4alkyl)-N(CH3)2, CO(piperazinyl), CONH-(C1-4alkyl)-pyrrolidinyl, CONH-(C1-4alkyl)-piperidinyl, CONH-(C i.4alkyl)-tetrahydropyranyl, morpholinyl, CO(pyrrolidinyl), CO(piperidinyl), CO(pyrrolidinyl), CH2-pyrrolidinyl, or CONH(cyclohexyl), wherein said J2 is optionally substituted with C1-4alkyl, CONH2, pyrrolidinyl, OH, O(C1-4alkyl), NH2, NH(C1-4alkyl), N(C1-4alkyl)2, -(C1-4alkyl)-CN, -(C1-4alkyl)-OH, -(C1-4alkyl)-N(C1-4alkyl)2,or CO(C1-4alkyl).
223. The method of claim 213, wherein R1 is phenyl;
R2 is phenyl, pyridyl, indole, furanyl, pyrazole, thiophene, tetrahydropyran, or indazole; J2 is halo, CN, NH2, C1-6alkyl, OR, SO2R, NHSO2R, COOR, CONH2, morpholinyl, -V'-R, or -(V2)m-Q, wherein
V1 and V2 are CO, -CONR-, -CONR-(C1-4alkyl)-, -CONR-(C |.4alky I)-OCH2-, -CONR-(C1-4alkyl)-N(CH3)-, R is H or C1 -4alkyl; and
Q is 1 ,4-diazepanyl, azetidinyl optionally substituted with OMe, piperidinyl optionally substituted with C1-4alkyl, 4-CH2OH, CONH2, pyrrolidinyl, OH, or CH2-pyrrolidinyl; piperazinyl optionally substituted with CH2CH2CN, CH3, COCH3, pyrrolidinyl optionally substituted with dimethylamino, tetrahydropyran, C3-10cyclolkyl optionally substituted with OH; and J1 is C1-6alkyl, CN, halo, OR, NH2, SR, COR, CO2R, CONH2, SOR, SO2R, S(O)2NH2, OCOR, NRC(O)R, NRCOOR, NRSOR, NRSO2R, NRSO2NH2, OCONH2, or NRCONH2; 4-6 membered fully saturated monocyclic ring containing 0-2 heteroatoms selected from oxygen, nitrogen, or sulfur.
224. A method for inhibiting ATR in a patient comprising administering a compound of claim 167.
225. The method of any one of claims 192-224, wherein said method is used to treat cancer.
226. The method of claim 225, comprising administering to said patient an additional therapeutic agent selected from a DNA-damaging agent; wherein said additional therapeutic agent is appropriate for the disease being treated; and said additional therapeutic agent is administered together with said compound as a single dosage form or separately from said compound as part of a multiple dosage form.
227. The method of claim 226, wherein said DNA-damaging agent is selected chemotherapy or radiation treatment.
228. The method of claim 226, wherein said DNA-damaging agent is selected from ionizing radiation, radiomimetic neocarzinostatin, a platinating agent, a Topo I inhibitor, a Topo II inhibitor, an antimetabolite, an alkylating agent, an alkyl sulphonates, an antimetabolite, or an antibiotic.
229. The method of claim 228, wherein said DNA-damaging agent is selected from ionizing radiation, a platinating agent, a Topo I inhibitor, a Topo II inhibitor, or an antibiotic.
230. The method of claim 229, wherein said platinating agent is selected from Cisplatin, Oxaliplatin, Carboplatin, Nedaplatin, or Satraplatin; said Topo I inhibitor is selected from Camptothecin, Topotecan, irinotecan/SN38, rubitecan; said Topo II inhibitor is selected from Etoposide; said antimetabolite is selected from methotrexate, pemetrexed, Thioguanine, Fludarabine, Cladribine, Cytarabine, gemcitabine, 6-Mercaptopurine, or 5-Fluorouracil; said alkylating agent is selected from nitrogen mustards, nitrosoureas, triazenes, alkyl sulfonates, Procarbazine, or aziridines; and said antibiotic is selected from Hydroxyurea, Anthracyclines, Anthracenediones, or Streptomyces family.
231. The method of claim 225, wherein said cancer is selected from lung cancer, head and neck cancer, pancreatic cancer and brain cancer.
232.A method of promoting cell death in cancer cells comprising administering to a patient a compound of formula I, wherein the variables are as defined in any one of claims 192-224.
233.A method of preventing cell repair from DNA damage comprising administering to a patient a compound of formula I, wherein the variables are as defined in any one of claims 192-224.
234.A method of inhibiting ATR in a biological sample comprising the step of contacting a compound of formula I with said biological sample.
235. The method of claim 234, wherein said biological sample is a cell.
236.A method of sensitizing cells to DNA damaging agents comprising administering to a patient a compound of formula I, wherein the variables are as defined in any one of claims 192-224.
237. Use of a compound of formula I as a radio-sensitizer or a chemo-sensitizer.
238. The method of any one of claims 232-236 wherein said cell is a cancer cell having defects in the ATM signaling cascade.
239. The method of claim 238 wherein said defect is altered expression or activity of one or more of the following: ATM, p53, CHK2, MREl 1 , RAD50, NBSl, 53BP1, MDCl or H2AX.
24O.The method of any one of claims 232-236, wherein said cell is a cancer cell expressing DNA damaging oncogenes.
241.The method of claim 238, wherein said cancer cell has altered expression or activity of one or more of the following: K-Ras, N-Ras, H-Ras, Raf, Myc, Mos, E2F, Cdc25A, CDC4, CDK2, Cyclin E, Cyclin A and Rb.
242. Use of a compound of formula I as a single agent (monotherapy) for treating cancer.
243. Use of a compound of formula I for treating patients having cancer with a DNA-damage response (DDR) defect.
244. The use according to claim 243, wherein said defect is a mutation or loss of ATM, p53, CHK2, MREI l , RAD50, NBSl , 53BP1, MDCl , or H2AX.
PCT/US2009/068827 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of atr kinase WO2010071837A1 (en)

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PL17205270T PL3354650T3 (en) 2008-12-19 2009-12-18 Compounds useful as inhibitors of atr kinase
NO09798991A NO2376485T3 (en) 2008-12-19 2009-12-18
ES09798991.7T ES2663222T3 (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as ATR kinase inhibitors
PL09798991T PL2376485T3 (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of atr kinase
RU2011129761/04A RU2604066C2 (en) 2008-12-19 2009-12-18 Pyrazine derivatives used as atr kinase inhibitors
NZ593969A NZ593969A (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of atr kinase
CN201610960262.1A CN106518856B (en) 2008-12-19 2009-12-18 Compounds useful as inhibitors of ATR kinase
CA2747252A CA2747252C (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of atr kinase
LTEP09798991.7T LT2376485T (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of atr kinase
CN200980155778.7A CN102300862B (en) 2008-12-19 2009-12-18 Compound as ATR inhibitors of kinases
KR1020117016218A KR101745331B1 (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of ATR kinase
KR1020177018220A KR101958632B1 (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of ATR kinase
AU2009327357A AU2009327357C1 (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of ATR kinase
SG2011044724A SG172248A1 (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of atr kinase
JP2011542506A JP5753093B2 (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of ATR kinase
MX2011006503A MX2011006503A (en) 2008-12-19 2009-12-18 Pyrazine derivatives useful as inhibitors of atr kinase.
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