WO1998020165A2 - Biallelic markers - Google Patents

Abstract

The invention provides nucleic acid segments of the human genome including polymorphic sites. Allele-specific primers and probes hybridizing to regions flanking these sites are also provided. The nucleic acids, primers and probes are used in applications such as forensics, paternity testing, medicine and genetic analysis.

Description

BIALLELIC MARKERS

RELATED APPLICATIONS

This application claims priority to U.S. provisional application Serial No. 60/030,455, filed November 6, 1996, the entire teachings of which are incorporated herein by reference .

BACKGROUND OF THE INVENTION

The genomes of all organisms undergo spontaneous mutation in the course of their continuing evolution, generating variant forms of progenitor sequences (Gusella, Ann . Rev. Biochem . 55, 831-854 (1986)). The variant form may confer an evolutionary advantage or disadvantage relative to a progenitor form or may be neutral. In some instances, a variant form confers a lethal disadvantage and is not transmitted to subsequent generations of the organism. In other instances, a variant form confers an evolutionary advantage to the species and is eventually incorporated into the DNA of many or most members of the species and effectively becomes the progenitor form. In many instances, both progenitor and variant form(s) survive and co-exist in a species population. The coexistence of multiple forms of a sequence gives rise to polymorphisms.

Several different types of polymorphism have been reported. A restriction fragment length polymorphism (RFLP) Is a variation in DNA sequence that alters the length of a restriction fragment (Botstein et al . , Am. J. Hum . Genet . 32, 314-331 (1980)). The restriction fragment length polymorphism may create or delete a restriction site, thus changing the length of the restriction fragment. RFLPs have been widely used in human and animal genetic analyses (see WO 90/13668; W090/11369; Donis-Keller, Cell 51, 319-337 (1987); Lander et al . , Genetics 121, 85-99 (1989) ) . When a heritable trait can be linked to a particular RFLP, the presence of the RFLP in an individual can be used to predict the likelihood that the animal will also exhibit the trait.

Other polymorphisms take the form of short tandem repeats (STRs) that include tandem di-, tri- and tetra- nucleotide repeated motifs. These tandem repeats are also referred to as variable number tandem repeat (VNTR) polymorphisms. VNTRs have been used in identity "and paternity analysis (US 5,075,217; Armour et al . , FEBS Lett . 307, 113-115 (1992); Horn et al . , W0 91/14003; Jeffreys, EP 370,719), and in a large number of genetic mapping studies. Other polymorphisms take the form of single nucleotide variations between individuals of the same species . Such polymorphisms are far more frequent than RFLPs , STRs and VNTRs . Some single nucleotide polymorphisms occur in protein-coding sequences, in which case, one of the polymorphic forms may give rise to the expression of a defective or other variant protein and, potentially, a genetic disease. Examples of genes, in which polymorphisms within coding sequences give rise to genetic disease include -globin (sickle cell anemia) and CFTR (cystic fibrosis) . Other single nucleotide polymorphisms occur in noncoding regions. Some of these polymorphisms may also result in defective protein expression (e.g., as a result of defective splicing) . Other single nucleotide polymorphisms have no phenotypic effects.

Single nucleotide polymorphisms can be used in the same manner as RFLPs and VNTRs, but offer several advantages. Single nucleotide polymorphisms occur with greater frequency and are spaced more uniformly throughout the genome than other forms of polymorphism. The greater frequency and uniformity of single nucleotide polymorphisms means that there is a greater probability that such a polymorphism will be found in close proximity to a genetic locus of interest than would be the case for other polymorphisms. The different forms of characterized single nucleotide polymorphisms are often easier to distinguish than other types of polymorphism (e.g., by use of assays employing allele-specific hybridization probes or primers) . Only a small percentage of the total repository of polymorphisms in humans and other organisms ha-s been identified. The limited number of polymorphisms identified to date is due to the large amount of work required for their detection by conventional methods. For example, a conventional approach to identifying polymorphisms might be to sequence the same stretch of DNA in a population of individuals by dideoxy sequencing. In this type of approach, the amount of work increases in proportion to both the length of sequence and the number of individuals in a population and becomes impractical for large stretches of DNA or large numbers of persons .

SUMMARY OF THE INVENTION

The invention provides nucleic acid sequences comprising nucleic acid segments of from about 10 to about 200 bases as shown in the Table, column 7, including a polymorphic site. Complements of these segments are also included. The segments can be DNA or RNA, and can be double- or single-stranded. Segments can be, for example, 10-20, 10-50 or 10-100 bases long. Preferred segments include a biallelic polymorphic site. The base occupying the polymorphic site in the segments can be the reference

(Table, column 3) or an alternative base .(Table, column 4) .

The invention further provides allele-specific- oligonucleotides that hybridize to a segment of a fragment shown in the Table, column 7, or its complement. These oligonucleotides can be probes or primers. Also provided are isolated nucleic acids comprising a sequence shown in the Table, column 7, or the complement thereto, in which the polymorphic site within the sequence is occupied by a base other than the reference base shown in the Table, column 3.

The invention further provides a method of analyzing a nucleic acid from an individual. The method determines which base is present at any one of the polymorphic sites shown in the Table. Optionally, a set of bases occupying a set of the polymorphic sites shown in the Table is determined. This type of analysis can be performed on a number of individuals, who are tested for the presence of a disease phenotype. The presence or absence of disease phenotype is then correlated with a base or set of bases present at the polymorphic sites in the individuals tested. DETAILED DESCRIPTION OF THE INVENTION DEFINITIONS

An oligonucleotide can be DNA or RNA, and single- or double- stranded. Oligonucleotides can be naturally occurring or synthetic, but are typically prepared by synthetic means. The oligonucleotides of the present invention can comprise all of an oligonucleotide sequence presented in column 7 of the Table or a segment of such an oligonucleotide which includes a polymorphic site. Oligonucleotides can be all of a nucleic acid segment as represented in column 7 of the Table; a nucleic acid sequence which comprises a nucleic acid segment represented in column 7 of the Table and additional nucleic acids (present at either or both ends of a nucleic acid segment of column 7) ; or a portion (fragment) of a nucleic acid segment represented in column 7 of the Table which includes a polymorphic site. Preferred oligonucleotides of the invention include segments of DNA, or their complements, which include any one of the polymorphic sites shown in the Table. The segments can be between 5 and 250 bases, and, in specific embodiments, are between 5-10, 5-20, 10-20, 10- 50, 20-50 or 10-100 bases. The polymorphic site can occur within any position of the segment. The segments can be from any of the allelic forms of DNA shown in the Table. Hybridization probes are oligonucleotides which bind in a base-specific manner to a complementary strand of nucleic acid. Such probes include peptide nucleic acids, as described in Nielsen et al . , Science 254, 1497-1500 (1991) . As used herein, the term primer refers to a single- stranded oligonucleotide which acts as a point of initiation of template-directed DNA synthesis under appropriate conditions ( e . g. , in the presence of four different nucleoside triphosphates and an agent for polymerization, such as, DNA or RNA polymerase or reverse transcriptase) in an appropriate buffer and at a suitable temperature . The appropriate length of a primer depends on the intended use of the primer, but typically ranges from 15 to 30 nucleotides. Short primer molecules generally require cooler temperatures to form sufficiently stable hybrid complexes with the template . A primer need not reflect the exact sequence of the template, but must be sufficiently complementary to hybridize with a template. The term primer site refers to the area of the target DNA to which a primer hybridizes. The term primer pair refers to a set of primers including a 5' (upstream) -primer that hybridizes with the 5' end of the DNA sequence to be amplified and a 3' (downstream) primer that hybridizes with the complement of the 3' end of the sequence to be amplified.

As used herein, linkage describes the tendency of genes, alleles, loci or genetic markers to be inherited together as a result of their location on the same chromosome. It can be measured by percent recombination •between the two genes, alleles, loci or genetic markers.

As used herein, polymorphism refers to the occurrence of two or more genetically determined alternative sequences or alleles in a population. A polymorphic marker or site is the locus at which divergence occurs. Preferred markers have at least two alleles, each occurring at frequency of greater than 1%, and more preferably greater than 10% or 20% of a selected population. A polymorphic locus may be as small as one base pair. Polymorphic markers include restriction fragment length polymorphisms, variable number of tandem repeats (VNTR's), hypervariable regions, minisatellites, dinucleotide repeats, trinucleotide repeats, tetranucleotide repeats, simple sequence repeats, and insertion elements such as Alu. The first identified allelic form is arbitrarily designated as the reference form and other allelic forms are designated as alternative or variant alleles. The allelic form occurring most frequently in a selected population is sometimes referred to as the wildtype form. Diploid organisms may be homozygous or heterozygous for allelic forms. A diallelic or biallelic polymorphism has two forms. A triallelic polymorphism has three forms. A single nucleotide polymorphism occurs at a polymorphic site occupied by a single nucleotide, which is the site of variation between allelic sequences . -The site is usually preceded by and followed by highly conserved sequences of the allele (e.g., sequences that vary in less than 1/100 or 1/1000 members of the populations) .

A single nucleotide polymorphism usually arises due to substitution of one nucleotide for another at the polymorphic site. A transition is the replacement of one purine by another purine or one pyrimidine by another pyrimidine. A transversion is the replacement of a purine by a pyrimidine or vice versa. Single nucleotide polymorphisms can also arise from a deletion of a nucleotide or an insertion of a nucleotide relative to a reference allele. Typically the polymorphic site is occupied by a base other than the reference base. For example, where the reference allele contains the base "T" at the polymorphic site, the altered allele can contain a "C", "G" or "A" at the polymorphic site.

Hybridizations are usually performed under stringent conditions, for example, at a salt concentration of no more than 1 M and a temperature of at least 25 °C. For example, conditions of 5X SSPE (750 mM NaCl, 50 mM NaPhosphate, 5 mM EDTA, pH 7.4) and a temperature of 25-30°C, or equivalent conditions, are suitable for allele-specific probe hybridizations. Equivalent conditions can be determined by varying one or more of the parameters given as an example, as known in the art, while maintaining a similar degree of identity or similarity between the target nucleotide sequence and the primer or probe used.

The term "isolated" is used herein to indicate that the material in question exists in a physical milieu distinct from that in which it occurs in nature. For example, an isolated nucleic acid of the invention may be substantially isolated with respect to the complex cellular milieu in which it naturally occurs. In some instances,-^" the- isolated material will form part of a composition (for example, a crude extract containing other substances) , buffer system or reagent mix. In other circumstance, the material may be purified to essential homogeneity, for example as determined by PAGE or column chromatography such as HPLC. Preferably, an isolated nucleic acid comprises at least about 50, 80 or 90 percent (on a molar basis) of all macromolecular species present.

I . Novel Polymorphisms of the Invention

The novel polymorphisms of the invention are listed in the Table. The first column of the Table lists the names assigned to the fragments in which the polymorphisms occur. The fragments are all human genomic fragments. The sequence of one allelic form of each of the fragments (arbitrarily referred to as the prototypical or reference form) has been previously published. These sequences are listed at http://www-genome.wi.mit.edu/ (all STS's (sequence tag sites)); http://shgc.stanford.edu (Stanford STS's); and http://ww.tigr.org/ (TIGR STS's). The Web sites also list primers for amplification of the fragments, and the genomic location of fragments. Some fragments are expressed sequence tags, and some are random genomic fragments. All information in the websites concerning the fragments listed in the Table is incorporated by reference in its entirety for all purposes.

The second column lists the position in the fragment in which a polymorphic site has been found. Positions are numbered consecutively with the first base of the fragment sequence as listed in one of the above databases being assigned the number one. The third column lists the base occupying the polymorphic site in the sequence in the data base. This base is arbitrarily designated -the-- reierence or prototypical form, but it is not necessarily the most frequently occurring form. The fourth column in the Table lists the alternative base(s) at the polymorphic site. The fifth column of the Table lists a 5' (upstream or forward) primer that hybridizes with the 5' end of the DNA sequence to be amplified. The sixth column of the Table lists a 3' (downstream or reverse) primer that hybridizes with the complement of the 3' end of the sequence to be amplified.

The seventh column of the Table lists a number of bases of sequence on either side of the polymorphic site in each fragment . The indicated sequences can be either DNA or RNA. In the latter, the T's shown in the Table are replaced by U's. The base occupying the polymorphic site is indicated in EUPAC-IUB ambiguity code.

II. Analysis of Polymorphisms A. Preparation of Samples Polymorphisms are detected in a target nucleic acid from an individual being analyzed. For assay of genomic

DNA, virtually any biological sample (other than pure red blood cells) is suitable. For example, convenient tissue samples include whole blood, semen, saliva, tears, urine, fecal material, sweat, buccal, skin and hair. For assay of cDNA or mRNA, the tissue sample must be obtained from an organ in which the target nucleic acid is expressed. For example, if the target nucleic acid is a cytochrome P450, the liver is a suitable source.

Many of the methods described below require amplification of DNA from target samples. This can be accomplished by e.g., PCR. See generally PCR Technology: Principles and Applications for DNA Amplifica tion (ed. H.A. Erlich, Freeman Press, NY, NY, 1992) ; PCR Protocols : A Guide to Methods and Applications (eds. Innis,-- et-al . , Academic Press, San Diego, CA, 1990); Mattila et al . , Nuclei c Acids Res . 19, 4967 (1991); Eckert et al . , PCR Methods and Applications 1, 17 (1991); PCR (eds. McPherson et al . , IRL Press, Oxford); and U.S. Patent 4,683,202.

Other suitable amplification methods include the ligase chain reaction (LCR) (see Wu and Wallace, Genomics 4, 560 (1989), Landegren et al . , Science 241, 1077 (1988), transcription amplification (Kwoh et al . , Proc . Na tl . Acad . Sci . USA 86, 1173 (1989)), and self-sustained sequence replication (Guatelli et al . , Proc . Nat . Acad . Sci . USA, 87, 1874 (1990)) and nucleic acid based sequence amplification (NASBA) . The latter two amplification methods involve isothermal reactions based on isothermal transcription, which produce both single stranded RNA (ssRNA) and double stranded DNA (dsDNA) as the amplification products in a ratio of about 30 or 100 to 1, respectively.

B. Detection of Polymorphisms in Target DNA

There are two distinct types of analysis of target DNA for detecting polymorphisms. The first type of analysis, sometimes referred to as de novo characterization, is carried out to identify polymorphic sites not previously characterized (i.e., to identify new polymorphisms) . This analysis compares target sequences in different individuals to identify points of variation, i.e., polymorphic sites. By analyzing groups of individuals representing the greatest ethnic diversity among humans and greatest breed and species variety in plants and animals, patterns characteristic of the most common alleles/haplotypes of the locus can be identified, and the frequencies of such alleles/haplotypes in the population can be determined. Additional allelic frequencies can be determined -for subpopulations characterized by criteria such as geography, race, or gender. The de novo identification of polymorphisms of the invention is described in the Examples section. The second type of analysis determines which form(s) of a characterized (known) polymorphism are present in individuals under test. There are a variety of suitable procedures, which are discussed in turn.

1. Allele-Specific Probes

The design and use of allele-specific probes for analyzing polymorphisms is described by e.g., Saiki et al . , Nature 324, 163-166 (1986); Dattagupta, EP 235,726, Saiki, WO 89/11548. Allele-specific probes can be designed that hybridize to a segment of target DNA from one individual but do not hybridize to the corresponding segment from another individual due to the presence of different polymorphic forms in the respective segments from the two individuals. Hybridization conditions should be sufficiently stringent that there is a significant difference in hybridization intensity between alleles, and preferably an essentially binary response, whereby a probe hybridizes to only one of the alleles. Some probes are designed to hybridize to a segment of target DNA such that the polymorphic site aligns with a central position (e.g., in a 15-mer at the 7 position; in a 16-mer, at either the 8 or 9 position) of the probe. This design of probe achieves good discrimination in hybridization between different allelic forms.

Allele-specific probes are often used in pairs, one member of a pair showing a perfect match to a reference form of a target sequence and the other member showing a perfect match to a variant form. Several pairs of probes can then be immobilized on the same support for simultaneous analysis of multiple polymorphisms within the same target sequence .

2. Tiling Arrays

The polymorphisms can also be identified by hybridization to nucleic acid arrays, some examples of which are described in WO 95/11995. One form of such arrays is described in the Examples section in connection with de novo identification of polymorphisms. The same array or a different array can be used for analysis of characterized polymorphisms. WO 95/11995 also describes subarrays that are optimized for detection of a variant form of a precharacterized polymorphism. Such a subarray contains probes designed to be complementary to a second reference sequence, which is an allelic variant of the first reference sequence. The second group of probes is designed by the same principles as described in the Examples, except that the probes exhibit complementarity to the second reference sequence. The inclusion of a second group (or further groups) can be particularly useful for analyzing short subsequences of the primary reference sequence in which multiple mutations are expected to occur within a short distance commensurate with the length of the probes (e.g., two or more mutations within 9 to 21 bases) .

3. Allele-Specific Primers An allele-specific primer hybridizes to a site on target DNA overlapping a polymorphism and only primes amplification of an allelic form to which the primer exhibits perfect complementarity. See Gibbs , Nucl eic Acid Res . 17, 2427-2448 (1989) . This primer is used in conjunction with a second primer which hybridizes at a distal site. Amplification proceeds from the -two-primers , resulting in a detectable product which indicates the particular allelic form is present. A control is usually performed with a second pair of primers, one of which shows a single base mismatch at the polymorphic site and the other of which exhibits perfect complementarity to a distal site. The single-base mismatch prevents amplification and no detectable product is formed. The method works best when the mismatch is included in the 3 ' -most position of the oligonucleotide aligned with the polymorphism because this position is most destabilizing to elongation from the primer (see, e.g., WO 93/22456).

4. Direct-Sequencing

The direct analysis of the sequence of polymorphisms of the present invention can be accomplished using either the dideoxy chain termination method or the Maxam Gilbert method (see Sambrook et al . , Molecular Cloning, A Laboratory Manual (2nd Ed., CSHP, New York 1989); Zyskind et al . i Recombinant DNA Laboratory Manual , (Acad. Press, 1988) ) . 5. Denaturing Gradient Gel Electrophoresis Amplification products generated using the polymerase chain reaction can be analyzed by the use of denaturing gradient gel electrophoresis. Different alleles can be identified based on the different sequence-dependent melting properties and electrophoretic migration of DNA in solution. Erlich, ed. , PCR Technology, Principles and Applica tions for DNA Amplification, (W.H. Freeman and Co, New York, 1992), Chapter 7.

6. Single-Strand Conformation Polymorphism Analysis

Alleles of target sequences can be differentia-ted using single- strand conformation polymorphism analysis, which identifies base differences by alteration in electrophoretic migration of single stranded PCR products, as described in Orita et al . , Proc . Na t . Acad . Sci . 86,

2766-2770 (1989) . Amplified PCR products can be generated as described above, and heated or otherwise denatured, to form single stranded amplification products. Single- stranded nucleic acids may refold or form secondary structures which are partially dependent on the base sequence. The different electrophoretic mobilities of single-stranded amplification products can be related to base-sequence differences between alleles of target sequences .

III. Methods of Use

After determining polymorphic form(s) present in an individual at one or more polymorphic sites, this information can be used in a number of methods. A . Forensics

Determination of which polymorphic forms occupy a set of polymorphic sites in an individual identifies a set of polymorphic forms that distinguishes the individual. See generally National Research Council, The Evaluation of Forensi c DNA Evidence (Eds. Pollard et al . , National Academy Press, DC, 1996) . The more sites that are analyzed, the lower the probability that the set of polymorphic forms in one individual is the same as that in an unrelated individual. Preferably, if multiple sites are analyzed, the sites are unlinked. Thus, polymorphisms of the invention are often used in conjunction with ~- polymorphisms in distal genes. Preferred polymorphisms for use in forensics are biallelic because the population frequencies of two polymorphic forms can usually be determined with greater accuracy than those of multiple polymorphic forms at multi-allelic loci.

The capacity to identify a distinguishing or unique set of forensic markers in an individual is useful for forensic analysis. For example, one can determine whether a blood sample from a suspect matches a blood or other tissue sample from a crime scene by determining whether the set of polymorphic forms occupying selected polymorphic sites is the same in the suspect and the sample. If the set of polymorphic markers does not match between a suspect and a sample, it can be concluded (barring experimental error) that the suspect was not the source of the sample. If the set of markers does match, one can conclude that the DNA from the suspect is consistent with that found at the crime scene. If frequencies of the polymorphic forms at the loci tested have been determined (e.g., by analysis of a suitable population of individuals) , one can perform a statistical analysis to determine the probability that a match of suspect and crime scene sample would occur by chance . p(ID) is the probability that two random individuals have the same polymorphic or allelic form at a given polymorphic site. In biallelic loci, four genotypes are possible: AA, AB, BA, and BB . If alleles A and B occur in a haploid genome of the organism with frequencies x and y, the probability of each genotype in a diploid organism is

(see WO 95/12607) : Homozygote: p (AA) = x²

Homozygote: p(BB)= y² = (1-x)²

Single Heterozygote : p(AB)= p (BA) = xy = x(l-x)

Both Heterozygotes : p (AB+BA) = 2xy = 2x(l-x)-

The probability of identity at one locus (i.e, the probability that two individuals, picked at random from a population will have identical polymorphic forms at a given locus) is given by the equation: p(ID) = (x²)² + (2xy)² + (y²)².

These calculations can be extended for any number of polymorphic forms at a given locus. For example, the probability of identity p(ID) for a 3-allele system where the alleles have the frequencies in the population of x, y and z, respectively, is equal to the sum of the squares of the genotype frequencies : p(ID) = x⁴ + (2xy)² + (2yz)² + (2xz)² + z⁴ + y⁴

In a locus of n alleles, the appropriate binomial expansion is used to calculate p(ID) and p(exc) .

The cumulative probability of identity (cum p(ID)) for each of multiple unlinked loci is determined by multiplying the probabilities provided by each locus. cum p(ID) = p(IDl)p(ID2)p(ID3) .... p(IDn) The cumulative probability of non-identity for n loci (i.e. the probability that two random individuals will be different at 1 or more loci) is given by the equation: cum p (nonID) = l-cum p(ID) . If several polymorphic loci are tested, the cumulative probability of non- identity for random individuals becomes very high (e.g., one billion to one) . Such probabilities can be taken into account together with other evidence in determining the guilt or innocence of the suspect .

B. Paternity Testing

The object of paternity testing is usually" to~determine whether a male is the father of a child. In most cases, the mother of the child is known and thus, the mother's contribution to the child's genotype can be traced. Paternity testing investigates whether the part of the child's genotype not attributable to the mother is consistent with that of the putative father. Paternity testing can be performed by analyzing sets of polymorphisms in the putative father and the child. If the set of polymorphisms in the child attributable to the father does not match the set of polymorphisms of the putative father, it can be concluded, barring experimental error, that the putative father is not the real father. If the set of polymorphisms in the child attributable to the father does match the set of polymorphisms of the putative father, a statistical calculation can be performed to determine the probability of coincidental match.

The probability of parentage exclusion (representing the probability that a random male will have a polymorphic form at a given polymorphic site that makes him incompatible as the father) is given by the equation (see WO 95/12607) : p(exc) = xy(l-xy) where x and y are the population frequencies of alleles A and B of a biallelic polymorphic site.

(At a triallelic site p(exc) = xy(l-xy) + yz (1- yz) + xz(l-xz)+ 3xyz (1-xyz) ) ) , where x, y and z and the respective population frequencies of alleles A, B and C) .

The probability of non-exclusion is p(non-exc) = l-p(exc)

The cumulative probability of non-exclusion (representing the value obtained when n loc-i a^re used) is thus : cum p(non-exc) = p (non-excl) p (non-exc2) p (non-exc3 ) .... p(non-excn)

The cumulative probability of exclusion for n loci (representing the probability that a random male will be excluded) cum p(exc) = 1 - cum p(non-exc) . If several polymorphic loci are included in the analysis, the cumulative probability of exclusion of a random male is very high. This probability can be taken into account in assessing the liability of a putative father whose polymorphic marker set matches the child's polymorphic marker set attributable to his/her father.

C. Correlation of Polymorphisms with Phenotypic Traits The polymorphisms of the invention may contribute to the phenotype of an organism in different ways . Some polymorphisms occur within a protein coding sequence and contribute to phenotype by affecting protein structure.

The effect may be neutral, beneficial or detrimental, or both beneficial and detrimental, depending on the circumstances . For example, a heterozygous sickle cell mutation confers resistance to malaria, but a homozygous sickle cell mutation is usually lethal. Other polymorphisms occur in noncoding regions but may exert phenotypic effects indirectly via influence on replication, transcription, and translation. A single polymorphism may affect more than one phenotypic trait. Likewise, a single phenotypic trait may be affected by polymorphisms in different genes. Further, some polymorphisms predispose an individual to a distinct mutation that is causally related to a certain phenotype.

Phenotypic traits include diseases that ha-ve teiown but hitherto unmapped genetic components (e.g., agammaglobulimenia, diabetes insipidus, Lesch-Nyhan syndrome, muscular dystrophy, Wiskott-Aldrich syndrome,

Fabry's disease, familial hypercholesterolemia, polycystic kidney disease, hereditary spherocytosis, von Willebrand' s disease, tuberous sclerosis, hereditary hemorrhagic telangiectasia, familial colonic polyposis, Ehlers-Danlos syndrome, osteogenesis imperfecta, and acute intermittent porphyria) . Phenotypic traits also include symptoms of, or susceptibility to, multifactorial diseases of which a component is or may be genetic, such as autoimmune diseases, inflammation, cancer, diseases of the nervous system, and infection by pathogenic microorganisms. Some examples of autoimmune diseases include rheumatoid arthritis, multiple sclerosis, diabetes (insulin-dependent and non-independent) , systemic lupus erythematosus and Graves disease. Some examples of cancers include cancers of the bladder, brain, breast, colon, esophagus, kidney, leukemia, liver, lung, oral cavity, ovary, pancreas, prostate, skin, stomach and uterus. Phenotypic traits also include characteristics such as longevity, appearance (e.g., baldness, obesity), strength, speed, endurance, fertility, and susceptibility or receptivity to particular drugs or therapeutic treatments.

Correlation is performed for a population of individuals who have been tested for the presence or absence of a phenotypic trait of interest and for polymorphic markers sets. To perform such analysis, the presence or absence of a set of polymorphisms (i.e. a polymorphic set) is determined for a set of the individuals, some of whom exhibit a particular trait, and some of which exhibit lack of the trait. The alleles of each polymorphism of the set are then reviewed--to-determine whether the presence or absence of a particular allele is associated with the trait of interest. Correlation can be performed by standard statistical methods such as a K - squared test and statistically significant correlations between polymorphic form(s) and phenotypic characteristics are noted. For example, it might be found that the presence of allele Al at polymorphism A correlates with heart disease. As a further example, it might be found that the combined presence of allele Al at polymorphism A and allele Bl at polymorphism B correlates with increased milk production of a farm animal.

Such correlations can be exploited in several ways . In the case of a strong correlation between a set of one or more polymorphic forms and a disease for which treatment is available, detection of the polymorphic form set in a human or animal patient may justify immediate administration of treatment, or at least the institution of regular monitoring of the patient. Detection of a polymorphic form correlated with serious disease in a couple contemplating a family may also be valuable to the couple in their reproductive decisions. For example, the female partner might elect to undergo in vitro fertilization to avoid the possibility of transmitting such a polymorphism from her husband to her offspring. In the case of a weaker, but still statistically significant correlation between a polymorphic set and human disease, immediate therapeutic intervention or monitoring may not be justified. Nevertheless, the patient can be motivated to begin simple life-style changes (e.g., diet, exercise) that can be accomplished at little cost to the patient but confer potential benefits in reducing the risk of conditions to which the patient may have increased susceptibility by virtue of variant alleles . Identification -of -a polymorphic set in a patient correlated with enhanced receptiveness to one of several treatment regimes for a disease indicates that this treatment regime should be followed.

For animals and plants, correlations between characteristics and phenotype are useful for breeding for desired characteristics. For example, Beitz et al . , US 5,292,639 discuss use of bovine mitochondrial polymorphisms in a breeding program to improve milk production in cows. To evaluate the effect of mtDNA D-loop sequence polymorphism on milk production, each cow was assigned a value of 1 if variant or 0 if wildtype with respect to a prototypical mitochondrial DNA sequence at each of 17 locations considered. Each production trait was analyzed individually with the following animal model:

Y_ijkpn= μ + YSi + P_j + X_k + β₁ + ... jS₁₇ + PE_n + a_n +e_p where Y_ijknp is the milk, fat, fat percentage, SNF, SNF percentage, energy concentration, or lactation energy record; μ is an overall mean; YSi is the effect common to all cows calving in year-season; X_k is the effect common to cows in either the high or average selection line; β to β_xl are the binomial regressions of production record on mtDNA D-loop sequence polymorphisms; PE_n is permanent environmental effect common to all records of cow n; a_n is effect of animal n and is composed of the additive genetic contribution of sire and dam breeding values and a Mendelian sampling effect; and e_p is a random residual. It was found that eleven of seventeen polymorphisms tested influenced at least one production trait. Bovines having the best polymorphic forms for milk production at these eleven loci are used as parents for breeding the next generation of the herd.

D. Genetic Mapping of Phenotypic Traits The previous section concerns identifying correlations between phenotypic traits and polymorphisms that directly or indirectly contribute to those traits. The present section describes identification of a physical linkage between a genetic locus associated with a trait of interest and polymorphic markers that are not associated with the trait, but are in physical proximity with the genetic locus responsible for the trait and co-segregate with it. Such analysis is useful for mapping a genetic locus associated with a phenotypic trait to a chromosomal position, and thereby cloning gene(s) responsible for the trait. See Lander et al . , Proc . Na tl . Acad . Sci . (USA) 83, 7353-7357 (1986); Lander et al . , Proc . Na tl . Acad. Sci . (USA) 84, 2363-2367 (1987); Donis-Keller et al . , Cell 51, 319-337 (1987); Lander et al . , Genetics 121, 185-199 (1989)). Genes localized by linkage can be cloned by a process known as directional cloning. See Wainwright, Med . J. Australia 159, 170-174 (1993); Collins, Nature Genetics 1, 3-6 (1992) .

Linkage studies are typically performed on members of a family. Available members of the family are characterized for the presence or absence of a phenotypic trait and for a set of polymorphic markers. The distribution of polymorphic markers in an informative meiosis is then analyzed to determine which polymorphic markers co- segregate with a phenotypic trait. See, e . g. , Kerem et al . , Science 245, 1073-1080 (1989); Monaco et al . , Na ture 316, 842 (1985); Yamoka et al . , Neurology 40, 222-226 (1990); Rossiter et al . , FASEB Journal 5, 21-27 (1991). Linkage is analyzed by calculation of LOD (log of the odds) values. A lod value is the relative likelihood of obtaining observed segregation data for a marker and a genetic locus when the two are located at a recombination fraction θ , versus the situation in which the two are not linked, and thus segregating independently (Thompson & Thompson, Genetics in Medicine (5th ed, W.B. Saunders

Company, Philadelphia, 1991) ; Strachan, "Mapping the human genome" in The Human Genome (BIOS Scientific Publishers Ltd, Oxford) , Chapter 4) . A series of likelihood ratios are calculated at various recombination fractions ( θ ) , ranging from θ = 0.0 (coincident loci) to θ = 0.50

(unlinked) . Thus, the likelihood at a given value of θ is: probability of data if loci linked at θ to probability of data if loci unlinked. The computed likelihoods are usually expressed as the log₁₀ of this ratio (i.e., a lod score) . For example, a lod score of 3 indicates 1000:1 odds against an apparent observed linkage being a coincidence. The use of logarithms- allows data collected from different families to be combined by simple addition. Computer programs are available for the calculation of lod scores for differing values of θ (e.g., LIPED, MLINK (Lathrop, Proc . Na t . Acad . Sci . (USA) 81, 3443-3446 (1984)) . For any particular lod score, a recombination fraction may be determined from mathematical tables. See Smith et al . , Ma thema tical tables for research workers in human genetics (Churchill, London, 1961); Smith, Ann . Hum . Genet . 32, 127-150 (1968) . The value of θ at which the lod score is the highest is considered to be the best estimate of the recombination fraction.

Positive lod score values suggest that the two loci are linked, whereas negative values suggest that linkage is less likely (at that value of θ ) than the possibility that the two loci are unlinked. By convention, a combined lod score of +3 or greater (equivalent to greater than 1000:1 odds in favor of linkage) is considered definitive evidence that two loci are linked. Similarly, by convention, a negative lod score of -2 or less is taken as definitive evidence against linkage of the two loci being compared. Negative linkage data are useful in excluding a chromosome or a segment thereof from consideration. The search focuses on the remaining non-excluded chromosomal locations .

IV. Modified Polypeptides and Gene Sequences The invention further provides variant forms of nucleic acids and corresponding proteins. The nucleic acids comprise one of the sequences described in the Table, column 8, in which the polymorphic position is occupied by one of the alternative bases for that position. Some nucleic acids encode full-length variant forms of proteins. Similarly, variant proteins have the prototypical amino acid sequences encoded by nucleic acid sequences shown in the Table, column 8, (read so as to be in- frame with the full-length coding sequence of which it is a component) except at an amino acid encoded by a codon including one of the polymorphic positions shown in the Table. That position is occupied by the amino acid coded by the corresponding codon in any of the alternative forms shown in the Table .

Variant genes can be expressed in an expression vector in which a variant gene is operably linked to a native or other promoter. Usually, the promoter is a eukaryotic promoter for expression in a mammalian cell. The transcription regulation sequences typically include a heterologous promoter and optionally an enhancer which is recognized by the host. The selection of an appropriate promoter, for example trp, lac, phage promoters, glycolytic enzyme promoters and tRNA promoters, depends on the host selected. Commercially available expression vectors can be used. Vectors can include host-recognized replication systems, amplifiable genes, selectable markers, host sequences useful for insertion into the host genome, and the like.

The means of introducing the expression construct into a host cell varies depending upon the particular construction and the target host. Suitable means include fusion, conjugation, transfection, transduction, electroporation or injection, as described in Sambrook, supra . A wide variety of host cells can be employed for expression of the variant gene, both prokaryotic and eukaryotic. Suitable host cells include bacteria such as E. coli , yeast, filamentous fungi, insect cells, mammalian cells, typically immortalized, e . g. , mouse, CHO, human and monkey cell lines and derivatives thereof. Preferred host cells are able to process the variant gene product to produce an appropriate mature polypeptide. Processing includes glycosylation, ubiquitination, disulfide bond formation, general post-translational modification, and the like. The protein may be isolated by conventional means of protein biochemistry and purification to obtain a substantially pure product, i . e . , 80, 95 or 99% free of cell component contaminants, as described in Jacoby, Methods in Enzymology Volume 104, Academic Press, New York (1984); Scopes, Protein Purifica tion, Principles and Practice, 2nd Edition, Springer-Verlag, New York (1987); and DeuLscher (ed) , Guide to Protein Purifica tion, Methods in Enzymology, Vol. 182 (1990). If the protein is secreted, it can be isolated from the supernatant in which the host cell is grown. If not secreted, the protein can be isolated from a lysate of the host cells.

The invention further provides transgenic nonhuman animals capable of expressing an exogenous variant gene and/or having one or both alleles of an endogenous variant gene inactivated. Expression of an exogenous variant gene is usually achieved by operably linking the gene to a promoter and optionally an enhancer, and microinjecting the construct into a zygote . See Hogan et al . , "Manipulating the Mouse Embryo, A Laboratory Manual," Cold Spring Harbor Laboratory. Inactivation of endogenous variant genes can be achieved by forming a transgene in which a cloned variant gene is inactivated by insertion of a positive selection marker. See Capecchi, Science 244, 1288-1292 (1989) . The transgene is then introduced into an embryonic stem cell, where it undergoes homologous recombination with an endogenous variant gene. Mice and other rodents are preferred animals. Such animals provide useful drug screening systems . In addition to substantially full-length polypeptides expressed by variant genes, the present invention includes biologically active fragments of the polypeptides, or analogs thereof, including organic molecules which simulate the interactions of the peptides. Biologically active fragments include any portion of the full-length polypeptide which confers a biological function on the variant gene product, including ligand binding, and antibody binding. Ligand binding includes binding by nucleic acids, proteins or polypeptides, small biologically active molecules, or large cellular structures.

Polyclonal and/or monoclonal antibodies that specifically bind to variant gene products but not to corresponding prototypical gene products are also provided. Antibodies can be made by injecting mice or other animals with the variant gene product or synthetic -peptide- fragments thereof. Monoclonal antibodies are screened as are described, for example, in Harlow & Lane, Antibodies , A Labora tory Manual , Cold Spring Harbor Press, New York (1988) ; Goding, Monoclonal antibodies, Principles and Practice (2d ed.) Academic Press, New York (1986) . Monoclonal antibodies are tested for specific immunoreactivity with a variant gene product and lack of immunoreactivity to the corresponding prototypical gene product . These antibodies are useful in diagnostic assays for detection of the variant form, or as an active ingredient in a pharmaceutical composition.

V. Kits The invention further provides kits comprising at least one allele-specific oligonucleotide as described above. Often, the kits contain one or more pairs of allele- specific oligonucleotides hybridizing to different forms of a polymorphism. In some kits, the allele-specific oligonucleotides are provided immobilized to a substrate. For example, the same substrate can comprise allele- specific oligonucleotide probes for detecting at least 10, 100 or all of the polymorphisms shown in the Table. Optional additional components of the kit include, for example, restriction enzymes, reverse-transcriptase or polymerase, the substrate nucleoside triphosphates , means used to label (for example, an avidin-enzyme conjugate and enzyme substrate and chromogen if the label is biotin) , and the appropriate buffers for reverse transcription, PCR, or hybridization reactions. Usually, the kit also contains instructions for carrying out the methods. The following Examples are offered for the purpose of illustrating the present invention and are not to be construed to limit the scope of this invention-.- T e teachings of all references cited herein are hereby incorporated herein by reference.

EXAMPLES

The polymorphisms shown in the Table were identified by resequencing of target sequences from three to ten unrelated individuals of diverse ethnic and geographic backgrounds by hybridization to probes immobilized to microfabricated arrays or conventional sequencing. The strategy and principles for design and use of such arrays are generally described in WO 95/11995. The strategy provides arrays of probes for analysis of target sequences showing a high degree of sequence identity to the reference sequences of the fragments shown in the Table, column 1. The reference sequences were sequence-tagged sites (STSs) developed in the course of the Human Genome Project (see, e . g . , Science 270, 1945-1954 (1995); Nature 380, 152-154 (1996)). Most STS's ranged from 100 bp to 300 bp in size. A typical probe array used in this analysis has two groups of four sets of probes that respectively tile both strands of a reference sequence. A first probe set comprises a plurality of probes exhibiting perfect complementarily with one of the reference sequences. Each probe in the first probe set has an interrogation position that corresponds to a nucleotide in the reference sequence. That is, the interrogation position is aligned with the corresponding nucleotide in the reference sequence, when the probe and reference sequence are aligned to maximize complementarily between the two. For each probe in the first set, there are three corresponding probes from three additional probe sets. Thus, there are four probes corresponding to each nucleotide in the reference sequence. The probes from the three additional probe -sets aaee identical to the corresponding probe from the first probe set except at the interrogation position, which occurs in the same position in each of the four corresponding probes from the four probe sets, and is occupied by a different nucleotide in the four probe sets. In the present analysis, probes were 25 nucleotides long. Arrays tiled for multiple different references sequences were included on the same substrate.

Multiple target sequences from an individual were amplified from human genomic DNA using primers for the fragments indicated in the listed Web sites. The amplified target sequences were fluorescently labelled during or after PCR. The labelled target sequences were hybridized with a substrate bearing immobilized arrays of probes. The amount of lable bound to probes was measured. Analysis of the pattern of label revealed the nature and position of differences between the target and reference sequence. For example, comparison of the intensities of four corresponding probes reveals the identity of a corresponding nucleotide in the target sequences aligned with the interrogation position of the probes. The corresponding nucleotide is the complement of the nucleotide occupying the interrogation position of the probe showing the highest intensity (see WO 95/11995) . The existence of a polymorphism is also manifested by differences in normalized hybridization intensities of probes flanking the polymorphism when the probes hybridized to corresponding targets from different individuals. For example, relative loss of hybridization intensity in a "footprint" of probes flanking a polymorphism signals a difference between the target and reference (i.e., a polymorphism) (see EP 717,113) . Additionally, hybridization intensities for corresponding targete-s from different individuals can be classified into groups or clusters suggested by the data, not defined a priori , such that isolates in a give cluster tend to be similar and isolates in different clusters tend to be dissimilar. Hybridizations to samples from different individuals were performed separately. The Table summarizes the data obtained for target sequences in comparison with a reference sequence for the individuals tested.

From the foregoing, it is apparent that the invention includes a number of general uses that can be expressed concisely as follows. The invention provides for the use of any of the nucleic acid segments described above in the diagnosis or monitoring of diseases, such as cancer, inflammation, heart disease, diseases of the CNS, and susceptibility to infection by microorganisms. The invention further provides for the use of any of the nucleic acid segments in the manufacture of a medicament for the treatment or prophylaxis of such diseases. The invention further provides for the use of any of the DNA segments as a pharmaceutical. All publications and patent applications cited above are incorporated by reference in their entirety for all purposes to the same extent as if each individual publication or patent application were specifically and individually indicated to be so incorporated by reference

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ATTGCACTGAAG I I I I I GAAATACCTTTGTAGTTACTCAAGCAGTTACTCCCTACACTGATGCAAGGA TTACAGAAACTGATGCCAAGGGGtC/G]TGAGTGAGTTCAACTACATGTTCTGGGGGCCCGGAGATAG ATGACTTTGCAGATGGAAAGAGGTGAAAATGAAGAAGGAAGCTGTGTTGAAACAGAAAAATAAGTC

WI-7718C 91 G AAAAGGAACAAAAATTACAAAGAACCATGCAGGAAGGAAAACTATGTATTAAT

ATTGCACTGAAG I I I I I GAAATACCTTTGTAGTTACTCAAGCAGTTACTCCCTACACTGATGCAAGGA TTACAGAAACTGATGCCAAGGGGCTGAGTGAGTTCAACTACATGTTCTGGGGGCCCGGAGATAGATG ACTTTGCAGATGGAAAGAGGTGAAAATGAAGAAGGAAGCTGTGTTGAAACAGAAAAATAAGTCAAA

Wl-7718b 248 AGGAACAAAAATTACAAAGAACCATGCAGGAAGGAAAACTATGTATT[A/G1AT

ATrGCACTG GTTTTTGAAATACCTTTGTAGTTACTCAAGC[A/C,ηGTTACTCCCTACACTGATGC AAGGATTACAGAAACTGATGCCAAGGGGCTGAGTGAGTTCAACTACATGTTCTGGGGGCCCGGAGAT AGATGACTTTGCAGATGGAMGAGGTGAAAATGAAGAAGGAAGCTGTGTTGAAACAGAAAAATAAG

Wl-7718a 42 TCAAAAGGAACAAAAATTACAAAGAACCATGCAGGAAGGAAAACTATGTATTA

AGGGAATTGTGTTGCTCCTGGAGGAAGCCCAGGCATCATTAAACAAGCCAGTAGGTCACCTGGCTTC CGTGGACCAATTCATCTTTCAGACAAGCTTTA[G/C]AGAAATGGACTCAGGGAAGAGACTCACATGC TTTGGTTAGTATCTGTGTTTCCGGTGGGTGTAATAGGGGATTAGCCCCAGAAGGGACTGAGCTAAACA

Wl-7227d 99 GTGTTATTATGGGAAAGGAAATGGCATTGCTGCTTTCAACCAGCGACTAATG Ul

00

AGGGAATTGTGTTGCTCCTGGAGGAAGCCCAGGCATCATTAAACAAGCCAGTAGGTCACCTGGCTTC CGTGGACCAATTCATCTTTCAGACAAGCTTTAGAGAAATGGACTCAGGGAAGAGACTCACATGCTTT GGTTAGTATCTGTGTTTCCGGTGGGTGTAATAGGGGATTAGCCCCAGAAGGGACTGAGCTAAACAGTG

WI-7227C 291 TTATTATGGGAAAGGAAATGGCATTGCTGCTTTCAACCAGCGACTAATGCAAT

AGGGAATTGTGTTGCTCCTGGAGGAAGCCCAGGCATCATTAAACAAGCCAGTAGGTCACCTGGCTTC CGTGGACCAATTCATCTTTCAGACAA[G/ηCTTTAGAGAAATGGACTCAGGGAAGAGACTCACATGC TTTGGTTAGTATCTGTGTTTCCGGTGGGTGTAATAGGGGATTAGCCCCAGAAGGGACTGAGCTAAACA

Wl-7227b 93 GTGTTATTATGGGAAAGGAAATGGCATTGCTGCTTTCAACCAGCGACTAATG

AGGGAATTGTGTTGCTCCTGGAGG[A G]AGCCCAGGCATCATTAAACAAGCCAGTAGGTCACCTGGC TTCCGTGGACCAATTCATCTTTCAGACAAGCTTTAGAGAAATGGACTCAGGGAAGAGACTCACATGC TTTGGTTAGTATCTGTGTTTCCGGTGGGTGTAATAGGGGATTAGCCCCAGAAGGGACTGAGCTAAACA

Wl-7227a 24 G GTGTTATTATGGGAAAGGAAATGGCATTGCTGCTTTCAACCAGCGACTAATG j CCACAATGCCTCTCCCACGATGTCAAGGACTCCTGTCTGTCCTGGAGGTGGGAGACAAGGAACCTCCG | AAGAGGAAGCAAGAAAGCCGTACTGTCTATGTTGTGATCCTTCATCGAACAAACTGATGCGAAAACT |TGAATCTGTTACTGAAATGAGGAGAGAAGGACATGTGCTATTGAACTGAGCCAAACACACTGTAAAT

Wl-7310b 234>A ATCCACAGACTCCCTCCCCTGCCCCCATCCCAfA/CIATGATCTTGAGATTTC

)

GAAGCAACCAGAAAGTATCTTTATCCCCATCTAGATTATGTCTGGGTTCTTCCAGACTCCTACGATTA AATTGTATGCATGTGAACAACTGATGAGGTACTTAGATCTCAGTGCTTTGCAGAAAGAAAAG[T/C]C GTCTACCATTTTCACCAAATTTCGTAGTACAATTTAAGTATCTCTTGTTATCTCCCCTAGGAGTCTAA

Wl-1 95b 1 30 AGTGAGCTGGGGAAGGCAGGATTT

GAAGCAACCAGAAAGTATCTTTATCCCCATCTAGATTATGTCTGGGT[T/C]CTTCCAGACTCCTACGA TTAAATTGTATGCATGTGAACAACTGATGAGGTACTTAGATCTCAGTGCTTTGCAGAAAGAAAAGTC GTCTACCATTTTCACCAAATTTCGTAGTACAATTTAAGTATCTCTTGTTATCTCCCCTAGGAGTCTAA

Wl-1795a 47 AGTGAGCTGGGGAAGGCAGGATTT

CACACAATTTGCAAACACTTCAAAGTGAACGCCCGACATCATCAGCCCGTTAACGTCCAGGCCATGT CCCACATAGAGAACGCTTTACTTCCACGTCTCTCCATACGTAGGTCCTGGTCTCCTATCACATTGCCA C[G/A]TAGCCCTCCCTTCCCTTCCCCCTACAGGCCCTCTTCAGGGCCCCAGTCCCCCTCTGAGACTCCC

1 36 ATGGATCATTCCTGTTTCTGTATCAGGCAGTGATTTAACTCC I I I I I I GT

CACACAATTTGCAAACACTTCAAAGTGAACGCCCGACATCATCAGCCCGTTAACGTCCAGGCCATGT CCCACATAGAGAACGCTTTACTTCCACGTCTCTCCATACGTAGGTCCTGGTCTCCTATCACATTGCCA C[G/A]TAGCCCTCCCTTCCCTTCCCCCTACAGGCCCTCTTCAGGGCCCCAGTCCCCCTCTGAGACTCCC

1 36 ATGGATCATTCCTGTTTCTGTATCAGGCAGTGATTTAACTCC I I I I I I GT

4-.

CACACAATTTGCAAACACTTCAAAGTGAACGCCCGACATCATCAGCCCGTTAACGTCCAGGCCATGT CCCACATAGAGAACGCTTTACTTCCACGTCTCTCCATACGTAGGTCCTGGTCTCCTATCACATTGCCA CGTAGC[C/ηCTCCCTTCCCTTCCCCCTACAGGCCCTCTTCAGGGCCCCAGTCCCCCTCTGAGACTCCC

1 41 ATGGATCATTCCTGTTTCTGTATCAGGCAGTGATTTAACTCC I I I I I I GT

CACACAATTTGCAAACACTTCAAAGTGAACGCCCGACATCATCAGCCCGTTAACGTCCAGGCCATGT CCCACATAGAGAACGCTTTACTTCCACGTCTCTCCATACGTAGGTCCTG[G/CJTCTCCTATCACATTG CCACGTAGCCCTCCCTTCCCTTCCCCCTACAGGCCCTCTTCAGGGCCCCAGTCCCCCTCTGAGACTCCC

1 1 6 ATGGATCATTCCTGTTTCTGTATCAGGCAGTGATTTAACTCC I I I I I I GT

CTCTTATTTCTCTGGGCACTGCTTTCTTTGGGGGCAAACTTCCAGTATCACT[G/A]ATACTAATATAA AAACCCTGT GTCTGCTTGCATTTTCAAGATTCAATATATATCCAGATTGTTTTCCCAGCAAAGAA TTTTATTTCTCAAGATATAAAAAATMATATTTAATTTCAGTTTCCTCAAAAGGAATATGAAATT

WI-1126C 52 G TGTTAAAATGCAAATCCAGCTGTAAC I I I I I I GGACTTGTCTTTTATTTCTT

CTCTTATTTCTCTGGGCACTGCTTTCTTTGGGGGCAAACTTCCAGTATCACTGATACTAATATAAAAA CCCTGTMGTCTGCTTGCATTTTCAAGATTCAATATATATCCAGATTGTTTTCCCAGCAAAGAAAATT TTATTTCTCAAGATATAAMMTMATATTTMTTTCAGTTTCCTCMAAGGAATATGMATTTGTT

Wl-1126b 230 AAAATGCAAATCCAGCTGT CTTTTT[T/C|GGACTTGTCTTTTATTTCTT

4-.

CGAGCTTGGGATAAAGCAAGGGGACCTTGGC[G/A]CTCTCAGCTTTCCCTGCCACATCCAGCTTGTTG TCCCAATGAAATACTGAGATGCTGGGCTGTCTCTCCCTTCCAGGAATGCTGGGCCCCCAGCCTGGCCA GACMGMGACTGTCAGGMGGGTCGGAGTCTGTAAMCCAGCATACAGTTTGGCTTTTTTCACATT

Wl-7038a 31 G . GATCA I I I I l ATATGAAATAAAAAGATCCTGCATTTATGGTGTAGTTCTGA

ATACGCTTTCTGTCTGTCCCACAGTGGAACCAGCACCCAGGTGGCCAGGGTCGGGCTCCACACA[G η CCCTCAGCCCCTTCAGCTTTGCATGTGTCCATCGGTGACTCAGCACAGAGTTTTCCAACCTCATGTGA CAAAAATACAGATTCCCAGTCTCCTCTCCTGGATTTGGATCTAGCAAGACCAGAGACGGTCCTAGAA

Wl-3429b 64 TCCTGACTGTTAACAAGCACTCCAGGCAATTCTTAAGACCAAGCACGGAGC

ATACGCTTTCTGTCTGTCCCACAGTGGAACCAGCACCCAGGTGGCCAGGGTCGGGCTCCACA[C/ηAG CCCTCAGCCCCTTCAGCTTTGCATGTGTCCATCGGTGACTCAGCACAGAGTTTTCCAACCTCATGTGA CAAAAATACAGATTCCCAGTCTCCTCTCCTGGATTTGGATCTAGCAAGACCAGAGACGGTCCTAGAA

Wl-3429a 62 TCCTGACTGTTAACAAGCACTCCAGGCAATTCTTAAGACCAAGCACGGAGC

ATTTTAGGACAGTGAAAAAAAGGGATTTATAAATAAAATCTATGCCATCCAGGAGGTATGTGTCAGT GTCCAGAACATCCTAGATGAAGTGGCTTCCTTTGGCGAAAGGATAAAGAAGTGAGTGACGGTGACCT GTGAGCCCCATTCTTCT[G/A]TGGGATAAGGTGTCCATTTGTTTCTTGGAGGGTGAAATGCCACATTC

WI-6786C 1 51 TTTTTGGCAGGGGACACTCCTTCTGGGTGCTCTATTGCTCAGTTTCATCATT 4-.

ATTTTAGGACAGTGAAAAAMGGGATTTATAAATAAAATCTATGCCATCCAGGAGGTATGTGTCAGT GTCCAGAACATCCTAGATGAAGTGGCTTCCTTTGGCGAAAGGAT[A/ηAAGAAGTGAGTGACGGTGA CCTGTGAGCCCCATTCTTCTGTGGGATAAGGTGTCCATTTGTTTCTTGGAGGGTGAAATGCCACATTC

Wl-6786b 1 1 1 TTTTTGGCAGGGGACACTCCTTCTGGGTGCTCTATTGCTCAGTTTCATCATT

ATTTTAGGACAGTGAAAAAAAGGGATTTATAAATAAAATCTATGCCATCCAGGAGGTATGTGTCAGT GTCCAGAACATCCTAGATGAAGTGGCTTCCTTTGGCGAA[A/ηGGATAAAGAAGTGAGTGACGGTGA CCTGTGAGCCCCATTCTTCTGTGGGATAAGGTGTCCATTTGTTTCTTGGAGGGTGAAATGCCACATTC

Wl-6786a 1 06 TTTTTGGCAGGGGACACTCCTTCTGGGTGCTCTATTGCTCAGTTTCATCATT

GGCTATTTGTAAATGCTTGGTTATTTGACTCCAAAATTGAATAAGTATTGGGGAAGAATCCCTCACCT ACTTCCAMTCCCTTACATATCMTTTTACACAAAGCCCCTAAACCTTCAGTTCCAATCACTCTGAAT TTCATATACCTCCATTATTAAATTCAATACATCATTGCAGAGAAAAGACAACGGTGCCAACTGGGTT

Wl-671 1 b 226 T TGGTTGGTGCCTGCACACCCACA[G ηTGGCAACTAAGTGTAATCTCTAAA

GGCTATTTGTA TGCTTGGTTATTTGACTCCAAAA[T/C]TGAATAAGTATTGGGGAAGAATCCCTC

ACCTACTTCCA TCCCTTACATATC TTTTACACAAAGCCCCTAAACCTTCAGTTCCAATCACTCT GAATTTCATATACCTCCATTATTAAATTCAATACATCATTGCAGAGAAAAGACAACGGTGCCAACTG

Wl-6711 a 36 T Ci - GGTTTGGTTGGTGCCTGCACACCCACAGTGGCAACTAAGTGTAATCTCTAAA

4-.

4-. ^1

4-.

00

4-.

Λ

O

CCTCCTCTGAG GCAGTTCTCTGAAAGACMTGGATTGTGGAGCATACTGMGACTATTCCTAMTGGCTATTTGTGTTG

TTTGTGTTGGG ATTTTCTGAAT GGTGGTCMG[A G]CTATTCAGAAMTCTCAGAGGAGGACAMTGATAGTGCACTGCAGCCAGCTCG

WI-11909 78 G TGGTCMG_ AG GACTGGCTTGCAAGAGTC

TCCTGTAMGC

CATGAAGAGT CAATTTTATAT AAAAATACCATTTAGCATCMTTGCCCCMGTTTGGCAGGCATGMGAGTGGGCAGTTCAΓT/G]GTT

WI-1 1806 60 GGGCAGTTCA ACTAATAA TTATTAGTATATMAATTGGCTTTACAGGMGCATTATGG

CCCTAGTGMTACMCCTTTGTCCTGGAGAC[C/A]CCAGCTAGTCTMGAAMCTTCCTAGGCTGAG

WI-11946 31 CTCTCTTGGGMTCTMGATAMGMCTGAGATCCTGGGMGMGGGM

TGMGATCAG

ATCTCTGGTTT CAGCTGTGGTG ACAAAATTCACMGTACAACACTGCTTATTTTCTTGCTTGMGATCAGATCTCTGGTTTATTTM[T/

WI-11965 65 G ATTT MTGTTGAT G]ATCMCATTCACCACAGCTGMGGAAATTAMCTGMCCT

TGCCCTACTAC TGAGGAAATGT ACCTATTTTGMACTGCAGAAAGGGCAGGACAAMCAAATCACTTCATAGATTTTTCTGGGAMTAT

GCTTTTAAAA GTTACAGTATT TGCCCTACTACGCTTTTAAMAA[T/A]AATAAAAATACTGTAACACATTTCCTCATTTCTCTTACGA

WI-11027 90 A TTTATT ATACTTTC I I I I I GATATTGCAMTTCTATGGCATACACAGAGGCACCTCCTCMTGCCCTG

TTCTGCTGAAGATCACAAMCMTTTCMCCTCTGTGGTTCAAMTMTTTMGGATCTTGTACCTTT

GTGTTTATTTTCTGTTTCAACTAAGGA[C/ηAGACTTCAGMGGCATAGCTTCCCTTGTAACGTTTTT

WI-1 1049 95 AAACATCTTTTTCATTTGTAGGAAGGMCATTTCAAAAGCCCAA CΛ )

AAAAGGACAG TTTCCATCTTA CCAGATATCA TTTCATTTCTG CAACATTTATCAAACATGGTAGGGAAMGTTCTCACTCTGCACTATAAAAAGGACAGCCAGATATCA

WI-15488 69 AC TMC AC[CtT]GTTACAGAAATGAAATAAGATGGAAAATm AACAAATTG

MCAGTTAAT GAMCACATC GGCTGGTGAM TGCTCAATTTMTGTGATAATCTCCMCAGTTMTGAAACACATCCGTA[A/G]GTATGACATCATTT

WI-13654 49 CGT TGATGTCAT CACCAGCCAGCTACTTCATGTGGCAGAAMGGTMCCTTTTCCCCATTTTACAGACAAMCCAGT _

ATGAGACCCTGCTTTGMCGTTAMCGTTTTGGMTMTGGAAAAGGAGCTAGGACMTTCTTGCTT

Wl- TCMGTAAMTTGTGACTGAGCAGAAMTCAGCCAGCTATCTTGGGTGCAGAGAGGTACTCCMGTA 1 1 070b 1 3_5j C| C[C, ]GTGGGGGTTCTGATGACTTCCACGGTCACTGGGGATCCMCAGMGGGM

CAGAAMTCA ATGAGACCCTGCTTTGMCGTTAMCGTTTTGGMTMTGGAAMGGAGCTAGGACMTTCTTGCTT

Wl- GCCAGCTATCT TFGGAGTACCT TCMGTAAMTTGTGACTGAGCAGAAMTCAGCCAGCTATCTT[G/ηGGTGCAGAGAGGTACTCCM 1 1070a 1 1 0 | G T T CTCTGCACC GTACCGTGGGGGTTCTGATGACTTCCACGGTCACTGGGGATCCMCAGMGGGM

MTCTTTTATATTTCCAGCTGTTGAGACAGTATTTTTGAGGGCTGATGTTACCTCTAGCGGCGAMCC AGAGCCAGCTATTMGCAGCCAGMAGCTACAGTMTTGMTACATGACCATT[T/C]CTCTTTTAGC

WI-12020 1 21 T C -- ACGTTCTTTGTTCTCCTC

Λ

4-.

/l

CΛ

00

O

ON 4-.

ON 00

-4

O

TGCTC I I I I I ATTTCACGTTTCACMCACACGCCGTG[G/ηTGGCACAGTCTACCAMGTGCCCGCAG CGCCACGCTTGGGCCGGMGGTCTCATTCTGTTCGTCTCTATGGACTGATTGMTTTGGGATGGCCAG CTCCAGMTGTTCCACGTGGGGGCACTCTGTGGGCAGAGAGGCTGAGCCCTTGCCCACACTGGCACCA

WI-9617 37 G MGAGGTTGCACGATGCAGCTTGCAGTGGGTCCMGCCGGGTGTGCTGTG

MTGCTGGAGAAMCATCMCATTGAGTTGACATTTGTTTTGCTGMGTATAGCTACCATCCACTAT CATGAATTTTTGTTTCATTACAMTGATAGAAMGCCAGATTCTCAAAATAAAG[T/G]ATMTTCTT TGTATTAAATAMTGTTTATAAATGTTTATGAAGCTCATTACATTATC I I I I I I AAMAAGTAAAAA

WI-9657 1 21 TTTTAGMCATATGACGCTTTTCATMTTMTGCTTTTGATATAGATTTGAGG

AAAAATTAAC CAGGGTCTTGCTCTGTCTCCCAGGCTAGAGTGAGGTGACACMTCMGACTCACAGTAGCCTCMCCT

Wl- CCTCCCMGTA CAGGTGTGGTG CCTATGCTCMGCCAGCCTCCCMGTAGCTGGGACTACAGGCATGT[G/C]ACACCACACCTGGTTM 131 19b 1 14ι G ' GCTGGGA T I I I l I I IM I I I I I l GTMAGATAGGGTCTCACTATGTTGCCCCGTCTCAAAAMCMACCMCTMC

CAGGGTCTTGCTCTGTCTCCCAGGCTAGAGTGAGGTGACACMTCMGACT[C/G]ACAGTAGCCTCA ACCTCCTATGCTCMGCCAGCCTCCCMGTAGCTGGGACTACAGGCATGTGACACCACACCTGGTTA

Wl- A l I I I I I I MI I I I I I GTAMGATAGGGTCTCACTATGTTGCCCCGTCTCAAAMACAMCCAACTAA 131 19a 51 C _

ACAGGAATCTGAAAGTTACCMGGCAATTTTCCCTTTTAGGATCATAMGACTACAGACTTMGCTT 3

TCATAMGAC TTAGAAATTTT TTTT[C/T]C I I I I I CCATATMTACACAAAATTTCTAAATATCCTTAAAAAAGAAAATATAAATAGT TACAGACTTA GTGTATTATAT TTCAGTATGTTATGTAGAGTCACATACTATGGCAAMATATTTTATTMTTGAGGGMTAGGCCMT

WI-13112 71 AGC I I I I I GGAAAMG

TGTTAACATTTTTATTGGTACGTGCTCTCAGTACM[C/A]AMCAGCATCAGTAGTGTACACTTTGAT

CAMGTGTACA MAMGGMTTTTTAGCTTAGTAGMMGMAGCCCAMGGTCAGAAGTATMTGMTATGTACAT

TGGTACGTGCT CTACTGATGCT CTTTATGGAMCTGTTTGTGTGACCATCTTTATCTTCCCCTGTGGATGAGATGTATGCACACACMGT

WI-12988 36 CTCAGTACM GTTT AAA

TGCTATTCATGACAGACACGTGAGACAMTATTCTTATTTTACAGATGGAMTAGACCCAGACATTA

CTMTAGTGG TTCAGTACTTTMCCACTAATAGTGGMCCCTGAGACTTTA[G/A]ATCTGCAMGGGGTTTAATAAT

Wl- MCCCTGAGA CATTATTAAAC GCMATATCACATATATTTCCA I I I I I AACACCATATTTAAGTTTTCCATTTTCTTMTAGAMATGA 13020a 1 08 GL CTTT CCCTTTGCAGA TAMAAATGTTTTCCCCMTAT

TGTATAAAMATCCMCTTGTTCCACMGTACATATGTCCTATGATTTTATGCATACATCCATATAC

CCATATACAT ATATATCAAGGTMAGTCCA[A/G]TACAMMMCAGCATTTCCTATGGCCAGTGTFCTACAGAAGT ATATCMGGT GCCATAGGM MGACTGTGCAMCTTTATCGTATAGTCAMTGAGATTGCACACTMGGCAGGATGAGGCAGMGCA

WI-12837 87' MAGTCCA ATGCTG I I I I I AGTTGTGTCCA

GTCCTCAGGCCCTTCTCTGGCTGCAGAGCCGTCTTCTCAGGTTGCCTGTC[G/C]TCTCCTGGCCTCTAG TCTTCCCTGCTCTCCGAGGTAGAGCTGGGTATGGATGCTTAGTGCCCTCACTTCTCTCTGTCTATACCT GCCCCATCTGAGCACCCATTGCTCACCATCAGATCMCCTTTGATΠTACATCATMTGTATTCACCA

L4261 1 b 50 CTGGAGCTTCACTTTGTTAC

GTCCTCAGGCCCTTCTCTGGCTGCAGAGCCGTCT[T/C]CTCAGGTTGCCTGTCGTCTCCTGGCCTCTAG TCTTCCCTGCTCTCCGAGGTAGAGCTGGGTATGGATGCTTAGTGCCCTCACTTCTCTCTGTCTATACCT GCCCCATCTGAGCACCCATTGCTCACCATCAGATCMCCTTTGATTTTACATCATMTGTATTCACCA

L4261 1 34 T CTGGAGCTTCACTTTGTTAC

TGAACGTGTGGTTAAAACTAGGCMTTGGTTMMATCMTTTMMAACAGGCCTAGAMCAGTG

TGMGAAATG ACCACACCTCMGCAATGATTATCCCTAGCACTCAGATTATGTTCTTGAMTACCATTTTCTGCTTTC GCTGATACCA ATGTGCATTTT AAMGAAAGACATGAGGGCTTCTTGMGAMTGGCTGATACCMG[CtηCTGCAGTGAAAMTGCA

Wl-1172b 1 79 A TCACTGCAG CATGATGAGCCTGGMCATGTTGT

TGAACGTGTGGTTMAA[C/A]TAGGCAATTGGTTAAAAATCAATTTAAMAACAGGCCTAGAAACA GTGACCACACCTCMGCMTGATTATCCCTAGCACTCAGATTATGTFCTTGAMTACCATTTTCTGCT TTCAAMGMAGACATGAGGGCTTCTTGMGMATGGCTGATACCMGCCTGCAGTGAAMATGCA

Wl-1 172a 1 7 CATGATGAGCCTGGMCATGTTGT

AGAGGCAGATTGGAAGTGTGAAAAAAATGAAAGM[G/C]MGMAAAAAGAGTCTAAATATTCAG 4-.

GCAGATTGGA CACTTACATTT MATGTMGTGCTGCCCTCMCTGTTCTTTACCCACTTMTTCTGCMTTTTGAAAACTAGATTGMT AGTGTGAAM CTGAATATTTA TCCTTTGCAAMCCCTTGCATCATGGATACCCGAGTTAMCCGTTMTTAAMGACATTAMCATGG

WI-1 177 35 G O A GACTCTTT CCTGGTG

TCCATGGTTTGGTTGCTACTGACTTTGTTAGCCTTACTGCCCACTATGCATTGGMCATTCCCATATTC CMCTMGCAGGAGTGTTCACMTM.ACMCATAGGCTCTTTATTCTCCTTCTTTCATTMTTTTCTT TCAC[GyA]TTATTCCCTCACCCTGMCGCCCTTCTTCCTTCGTAGTGACATTTTAAMTCCACTTTAC

Wl-1231 b 1 41 I G ACATTCGGACC

TCCATGGTTTGGTTGCTACTGACTTTGTTAGCCTTACTGCCCACTATGCATTGGMCATTCCCATATTC

GGCTCTTTATT CMCTAAGCAGGAGTGTTCACMTMACMCATAGGCTCTTFATTCTCCTTCTTTCA[T/C]TMTTTT

CTCCTTCTTTC CGTTCAGGGTG CTTTCACGTTATTCCCTCACCCTGMCGCCCTTCTTCCTTCGTAGTGACATTTTAAMTCCACTTTACA

Wl-1231 a 1 26 T!C A_ __ _ AGGGAATM CATTCGGACC

ACATACATAT GMGGCAGGACTGTGTTTTGGAGGACMMAGTAAMTC I I I I l ATATCTTTA I I I I I I MTTTTATT [CCATTATACA GACCTTTCTTT TTTTTTCAGGCATATAGACATACATATCCATTATACMCAGMMG[G/C]GGGCTGGAAAAGMAG

WI-472 1 1 4 G C ACAGAMAG TCCAGCCC GTCMGTGAGATTTCAGATATTCTTAAATGCMGGCTGACAMTTTGGGCTTGATT

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TΓTTTGTTTΌCTCTGGACACCCACTGCTCCCAGGATGAMGGAGAG[G/A]MTGAGATCAGTTTTGGA

WI-7593 46 CACTTCCTCTTGAMTATAMGMTCMCMGTFACAGTCATGTTGGGGACTTCTTCTCTCTCCM

AGTGCATCTTGGGGGAMGGGCTCCAGTGTTATCTGGACCAGTTCCTTCATΠTCAGGTGGGACTCTT GATCCAGAGA[A G]GACAMGCTCCTCAGTGAGCTGGTGTATMTCCMGACAGMCCCMGTCTCC TGACTCCTGGCCTTCTATGCCCTCTATCCTATCATAGATAACATTCTCCACAGCCTCACTTCATTCCAC

WI-6962 78 CTATTCTCTGAAMTATTCCCTGAGAGAGMCAGAGAGATTTAGATMGA

GCAGAGMGAGMCCATGCCAGGGGAGMGGCACCCAGCCATC[C/G]TGACCCAGCGAGGAGCCM

MGGCACCCA GCTCCTCGCTG CTATCCCAMTATACCTGGGTGMATATACCAAATTCTGCATCTCCAGAGGMMTMGAMTMA

WI-7059 43 GCCATC GGTCA GATGAATTGTTGCAACTCTTAAAAAM

CACTTCACTGA MGACACCAT TCTACTTTCTG AGCAGCCATCACATGATCTGTTTTTCACCACTTCACTGAMGACACCATTTAT[A/C]TACCCMGGG

WI-9063 53 CCCTTGGGT CAGAMGTAGMCTTACTATTCATTAMTGTTTGACACMTTGGMTTGTC

MGGGGCATTGAGACTATAMGCAGTAGACAATCCCCACATACCATCTGTAGAGTTGGMCTGCATT CTTTTMAGTTΓΓATATGCATATATTTΓAGGGCTGCTAGACTTACTTTCCTATTTTC^'FTTTCCATTGC TATTCTTGAGCACAMATGATMTCMTTATTACATTTATACATCACC I I I I I GACTTTTCCMGCCC

WI-7079 293 TTTTACAGCTCTTGGCATΠTCCTCGCCTAGGCCTGTGAGGTMCTGGGAT

GGTAAMGTT GACAGAI I I I I o CTTTTTGCTCT GACCTAGTTCC TGGATGCCGAGGTAAMGTTCTTTTFGCTCTAAAAGM[A/G]AAGGMCTAGGTCAAAMTCTGTCC 1

WI-9074 38 AAAAG TT GTGACCTATCAGTTATTM I I I I I MGGATGTTGCCACTGGCAMTGTMCTGT

GGAGTTTGCCCCTTCCTMGGGMGGAGATCTTTATCTTTCTGGTTGGCTTGACCAGTCACGTTGGGA GMGAGAGAGAGTGCCAGGAGACCCTGAGGGCAGCCGGTTCCTACTTTGGACTGAGAGMGGGAGCC CCAGGCTGGAGCAGCATGAGGCCCAGCMGMGGGCTTGGGTTCTGAGGMGCAGATGTTTCATGCT

Wl-7104b 249 GTGAGGCCTTGCACCAGGTGGGGGCCACAGCACCAGCAGCATCTTTG[CtFJF

GGAGTTTGCCCCTTCCTMGGGMGGAGATCTTTATCTTTCTGGTTGGCTTGACCAGTCACGTTGGGA GMGAGAGAGAGTGCCAGGAGACCCTGAGGGCAGCCGGTTCCTACTTTGGACTGAGAGMGGGAGCC CCAGGCTGGAGCAGCATGAGGC[C/A]CAGCMGMGGGCTTGGGTTCTGAGGMGCAGATGTTTCAT

WI-7104 1 57 GCTGTGAGGCCTTGCACCAGGTGGGGGCCACAGCACCAGCAGCATCTTTGCT

CCTGAGCCCTC TGTAGGGCTGA CATACMTGAGAGCCCTGAGCCCTCMGMCTCA[CtηGCCAGCTCAGCCCTACACCAGTTTCCACC

WI-8974 34 AAGMCTCA GCTGGC TGGAGTTCATGCMGGGCMMGGCAGTGCCATGCMGCTGTTTM

GCTTACAGGAG

CCTMGCATTG AGACTAGACA CTGTGAGGGTGACGTTAGCATTACCCCCMCCTCATTTTAGTTGCCTMGCATTGCCTGGC[Cπ TC

WI-9161 61 1 CCTGGC GGM CTGTCTAGTCTCTCCTGTMGCCAMGMATGMCATTCCA

CCCTGTTCCCATGCTGACCTGTGTTTCCTCCCCAGTCATCTTTCCTGTTCCAGAGAGGTGGGGCTGGAT

WI-9014C 93 lT Cl- GTCTCCATCTCTGTCTCMCTTTAΓF/CIGTGCACTGAGCTGCMCTTCT

CCCTGTTCCCATGCTGACCTGTGTTTCCTCCCCAGTCATCTTTqCtFJFGTTCCAGAGAGGTGGGGCTG

Wl-9014b 44 GATGTCTCCATCTCTGTCTCMCTTTATGTGCACTGAGCTGCMCTTCT

TCTGAGAGAMTGACTTGTGGGAGACACCCTGCAGATCCTCATGGGTTTGTGACAGACCCTGCGTGCT CAGTGCCCTTTMGTGCATCCCGCTGTGCTGACTTTGAGTGGGATCMCATCTGTCCTACGGGTCCCC TCI I I I I IGGCCCCAGTATTCATGGCAGGGTTTGTTGGACACCTACTAGCTTCCCTTCCCATTCMCAC

Wl-7023b 206 A[C/A]ACACACATTCTTGCTCTACCCAMGCTCTGGCTGGCAGCACTM

TCTGAGAGAMTGACTTGTGGGAGACACCCTGCAGATCCTCATGGGTTTGTGACAG[A/C]CCCTGCGT GCTCAGTGCCCTTTMGTGCATCCCGCTGTGCTGACTTTGAGTGGGATCMCATCTGTCCTACGGGTC CCCTC I M IT IGGCCCCAGTATTCATGGCAGGGTTTGTTGGACACCTACTAGCTTCCCTTCCCATTCM

Wl-7023a 56 CACACACACACATTCTTGCTCTACCCAMGCTCTGGCTGGCAGCACTM

CTGAMTCCCCCTCTCTGCCCTGGCTGGATCCGGGGACCCCTTTGCCCTTCCCT[CT]GGCTCCCAGCC CTACAGACTTGCTGTGTGACCTCAGGCCAGTGTGCCGACCTCTCTGGGCCTCAGTTTTCCCAGCTATG AAAACAGCTATCTCACAMGTTGTGTGMGCAGMGAGAAMGCTGGAGGMGGCCGTGGGCCMT

WI-7093 54 GGGAGAGCTCTTGTTATFATTMTATTGTTGCCGCTGTTGTGTTGTTGTTA

ACATATCTGAAAAATGTTGAMGCCTMGCCAGGAATAMAGAAMGTAGAGATMTAATCA[G/A]

WI-9171 62 TTCTTTACMCCGATGGTMTTMGCTTGTATTCACMGACTTCATGC

CTAGGACCCC TCTAGAGGGTA vo 00 ATTCTCCTATT TATAGGACAGG GTGTGAGACCATCATGGTGCCAGTCTAGGACCCCATTCTCCTATTTAΓT/C]CAGTCCTGTCCTATATA

WI-9174 47 T ACTG_ CCCTCTAGAMCAGAMGCMTTTTTAGGCAGCTATGGTCAMTTGAG _ _

CAGAGGTCTTG MGGCCAGATGCACATCCCTGGMGGACATCCATGTTCCGAGMGMCAGAT[A/G]ATCCCTGTATT

CCATGTTCCGA AMTACAGGG TCMGACCTCTGTGCACTTATTTATGMCCTGCCCTGCTCCCACAGMCACAGCMTTCCTCAGGCTA

WI-7753 52 GMGMCAGA A AGCTGCCGGTTCTTAMTCCATCCTGCTMGTTMTGTTGGGTAGM

AMGGGMAG

CCACTTCTCCC TCTGACCTAGG MAGMCTACAGAGGACGATGTCCAAMCMAAMTGGCATCACCTGTCAAAMTGGAGTTCCACT

WI-9186 76 CGCA T TCTCCCCGCA[G/A]ACCTAGGTCAGACTTTCCCTTTCATCTT

AGMTATTGT

CTGCCTTAMG GGTGTGTGTGG TTGGACAMCCTAGMTTTTCTCCCTFTATGTATCTCTATCGATTGTGTAGCMTTGACAGAGMTM

WI-9193 94 G, CA TAGGGGG CTCACMTATTGTCTGCCTTAMGCA[G/A]TACCCCCCTACCACACACACCCCTGTCCTC

TTTGGATTGATATCGTGAMTCCTCAGCCGAGAMTTGGGCTGGATTG[CtF]GCTTTGGTTMTACAT

WI-9015 48 CTTTCCCTMAGMGATAMCACAAMTCCATTCCAGGTAGCTCGGCACCMCTMGM

GGAGCCAGGAGACAGCAGGGTCTGAGAGAGGAGCCAC[A/G]GTCCCTMTGACACCCACTCCTAGCC

GGTCTGAGAG GGAGTGGGTGT CTGAGGCTCGTGCCCCTCAGACTGGGGMGAGTCCMGGMGGGAGGGAGCAGCCACTCCTCMTGC

WI-7254 37 AGGAGCCAC CATFAGGGA TCMTGGCTCCCCTGMATCMGACAGG

o β

CMGAGAGAG TGCAAAGAAA CCAGGAGCACTAGAGAGGGAGGGGGMGAGCAGMGTTAGAGAAAAAMGCCACCGGAGGAMGG AGAGGAMGA GMTGAAAGTT AAAAAACATCGGCCAACCTAGAMCGTTTTCATTCGTCATTCCMGAGAGAGAGAGGAMGMAM

WI-7424 1 31 AAAA G [T/A]ACAACTTTCATTCTTTCTTTGCACGTTCATAMCATTCTACATA

TCCTGCMGMGTTCTCMGCC I I I I I GATTTTTGTGCMTMAGTACAGCTTTGCATMGAGTGAM TTGGGCTAGCTTAMTGGATCCATAMCTTTCTTCTMTTTTMGTGAGA[A/C]TCTTTTAMCACCT GTTMATTTMTGTAGCAGTCTGAGAATCTAAMTTATGTACCACTCGTTTATFTGTTCATTCATCCA

X86400 1 1 8 TCCCTTTTCCCATGMTATTTCA

GTGGCCACTACATGTTATAGAMCCATCATCTTGTCACACAGCACAGTCTATGMTMMGGCTGAG TTATCACTMGCAGGAGAAAMGCATTAAAMGTGTCCCATTMMGGGACTTTTMTCMCCTAA TMACTCTMTTCTGCTGACTTTTTAMGATCTMGGTCATTFTMTACATGCTGAAMGGGTCACA

WI-8053 242 T ATTAATTCTTTGATCTTTTTTACTCACTGTTAACTTATATAA[T/A]TTCAGMC

TACACMTGMTTGCTTTTATTTCGGTATGCATCCACATTTCAGCATTTAGTGGTCCTGMCAGCMG TGGMAGACGCAGCMTTTGCCAGGAGGTCMGCCCACCMTTTCGGGGATCTGCTGTGCACACCGG GTTCCTTCTTMTCCCTGCTGAGGATCTTG[G/A]GMGCAGCAGCAGCACCAMACCMGGCATGCA

WI-6190 ' 1 65 CCGGATTCMGGTTCTTTTTGTTCCAGTTGTCAGATTCCAMCTAGACCCCA

MCAGTCACCACCMCCACATGACMCTCGCCAGGCMGGCCTTGCTTCCCTCCCTCCTTTGCGTCCC ATGTGCCTAGTCAGCMGGTCGGGGAGGCACCGATGTTAGCTTCGCCCAMGGGAGTATTACAGAGA GAGGCTTGGGAM[G C]GGMGGMACCTGGACAGGCTTTTCAGCACTGAGAMTCACTTAMACTG

WI-6275 I 1 48 G Cl ATTTGCTTTCAGTMCTGGTATGTCTGM

ACCMGAGATCAGCTGTCTAMCAGCAGCT rGATTGT[G/ηGGGCTTCCTGAMGMACCTTGC

TGACAGCTTCTCACTGACCTGCAGGACGGMCCGTACCTGAGAGGGGATGGGGGCTCTCTCACAAM GMTATTTGGGGCAGMCCCTGGMCTGGCCACCAGGGACATCCCAMTATCCCCTCCTCCTCAGGG

WI-6421 41 CTCACCCCGACATCCTCAGCCAMTGMGGCTCTGM

GGGTGAGACGGGTTTATTGTGCACATFTACACAGCGTCACAGCGTCTGGGCTGGCAGCGGCCATGCTC CTGTGGTCGGGCTGCTCTACMGGGCGTTCACTTTTCTTCACCACACTATGTACAGTCAGTGCTCCM GGTGATGGGCTACAGTGCTGCATCAGTGAGTCTGTACACACATTTTTACATAMTTACACACGACTC

WI-6905 21 51 T j A ATACATGMAAA[T/A]AGAGCCTAAGGGCCTGTATTTTAATGAGAAMAAA

MCTTGTTTACAAMTAGGCTTTGCAMCTTCATTACTGMTTGTMAGTCMTGACTGTGTTGTTTT TAAAATATGTACCMGGAMTACAMTTGGATMTGATCATTTTTCATGCTCAGGAGAGMCAGCAC AGAAATAMGGATACTGCACMGGTGCMGGAAACCGGMCCCATTGTGTACACTGTCTTCACACAG

WI-9420 202 G A' — [G/A]GCATTCTTTCTCACCTTMCTGCAGCTGTGCMGATGCCTCAGTGTG

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TTTCTAGGCTGTACAGTCTGATGCATGA I I I I I I I ATAMTATTTCATACTCTTGTGMTTTGGATCTT TTTACTTTGAGCATATATTTTAGMTATGTGT[A/G]TGTTAMGGATCTCCACMTGTCTGCAGTGTG MGGCAGGTTCATTGTGGMTAGTTTMCAGTCAGGMGGCTAMCTGGTCAGTATTMTGTGTAGC

WI-7805 1 01 G CCTACCAMAATAGCCAGTAGTATCTGAAMTGMAAATAMTGMGTAT

GGCCAGGAGATTAGCMCMGGATTCATTCTGTTACTTACTTGCCCCTTTTTATCTTTCCCTCTTGCCC CAGTCCCTTCTCTCCAGCTTCATGTGMGCTCTGCACAGACMGACACTCAGTGTCCTTGGCAGTGCT [G/T]CTACTCCTCAGGTGCAGCATACATMCCAGTMGAGACTMATCTGCMTATATMAGAGCTC

WI-7416 1 37 CTACAMTCAGTAACATGMGMCACTCMMATTGGCAMTGTCATCAG

ATTTGMGATTTGGAGGGCTTTGCAGAGGAAMTAGATTTCMTTGGATCCCCAAACTATMTGACA AG I I I I I MTTAGGTGTGATCMGGCTFCTMMGTGAMTGCMGTTGTTACCAGTAMGTTTATA TCTTCCATTCAGCCCAGCTCATTTGCCAGAAMTTCAGGTGAGTGGATTGGCCAGACTATCTGGCMG

WI-140 252 GATGAAAATTTTAGTTTAMMJGTGTCATTTGTCTGTAπGGCAπ

GAGGTCTTTCAGCMCATGGMGCCCTACTGCTTCMCCCCGAGTTCCCCGGATCMGTGCTGGCACC CATGATGGAAACTCTTGCCATGGTTTTAGTACCCTGGACCMGTAGTCATTCCATCCTGACTTTAAM TTCTMACAGCCTTTGATGGGACMTCTCTGCTMAGACTMCCACTTCCTTATCTTATCTTCAGCTA

WI-198 21 8 CCTGCTTCCCTTTC[C/ηGTTTAACMAGCATAGMTATTCTGMCMCT

TTCATGGTCCCAAGACAGATTTTAMGAAAGAAMTAAGCCTCATCTCCTMCTATGACTTGGTCGG o ON MGCCMGAACCTACTTCAACATTTGACCCATMCCTTCTCTTGAGATGATGGGCTGACTTTTTCMT GCATGAGTTTGΓT/C]CCAAAGGCTTGATGGGAAMTCTCMCATTTGTTACCTMGMAGAGGATGT

WI-205C 1 46 ATCTTACTTTGTTTAAMMCTGCATATGCCTTTA I I I I I GTTTTAGTTCCC

TTCATGGTCCCMGACAGATTTTAMGMAGAAMTMGCCTCATCTCCTMCTATGACTTGGTCGG MGCCMGMCCTACTTCMCATTTGACCCATMCCTTCTCTTGAGATGATGGGCTGACTTTTTCMT GCATGAGTTTGΓT/C]CCAMGGCTTGATGGGAAMTCTCMCATTTGTTACCTMGMAGAGGATGT

Wl-205b 1 46 ATCTTACTTTGTTTAAAAMCTGCATATGCCTTTATTTTTGTTTTAGTTCCC

GMGACTGAGTTTCCAGGAGGTTGCAGCCGTTTCTCTCGGGCCATATGGCTMTMGGAGCTTGAGCA GGGATFCMCCTGTTTGCMCCCMGTNCTTTCCMGAGGTCTCAGACTACCTCCTCCATCTCCCCCT CTCCCCCACMCACACAMTACAGAGATT[G/C]MTTCAGGAGCCAGTTTCTAGGTGGGCTTTGAGC

WI-234 1 65 MTCATACACAGTMTCTCTTGGTGCTTTAGTTTTCTCAMTGGGMATGG

AGCTTTTGAMTCCAAMACCACAT[A/G]CTTGACTCTCTTATCCTCCTCTTGTTGTMCATCTATCC CTGAGGCAGAAMTACAGMCACCCTGTGGCTGCCTGMCGGAGGMGGATGGGGGCGGGGAGACAT CGGTCMTGTATCAMGCATCTCTCTGCCTGAMGACCTCTCCTGAMGACATGAGCTATTAGGAGC

Wl-276b 25 A G — TCTGGCMGGGCTTTGTCTTATCCTCCTTGCTATCCCTGATGACTGGGCAM

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TGTTCTCTGGTCCAGGCACCGGGCTMGTCTTGTCTGCATMTGGMTMTCMCTGGACMCCCCNG CTNAGGTAGGNTACCTNGGCMTTAGCCCCATCTTACAGCTGCAMAGAGG[C/T]GCTCTGAGAGGT AMGTGCCCTGCCCCMCGCGCACMCTAGAGAGCAGCCAMCAGGTGTTTGMCCCAGCTCTGCCT

WI-1900 1 1 9 GACTTCAGATCTGTGTGCTTMCTGCCATGAGAMCCACTTTTCTTTGCTCC

ATTCCAGTTTCACAGTGGGCACAGGAGTCAGATTAGGGCTMGTTGGGGGGACAGGATGCACAGCGT GTTGGCTCAGGATCTCTGGGAGGTGGCACCTGTGACCTGGGCTMNCATGCTACTTTCAGAGTCMGC AGCMGCCMTGGGTAGGGAMGACCAGCC[C/T]CTCTGMNCTGGGTCCCACGTGGAGATAGTGM

WI-1943C 1 65 TACAGGGCACCGNTGAGCATTCCAGATGACTCCAMGCCCCGGCTGGAGTAT

ATTCCAGTTTCACAGTGGGCACAGGAGTCAGATTAGGGCTMGTTGGGGGGACAGGATGCACAGCGT GTTGGCTCAGGATCTCTGGGAGGTGGCACCTGTGACCTGGGCTMNCATGCTACTTTCAGAGTCMGC AGCMGCCMTGGGTAGGGAMGACCAGCC[C/T]CTCTGMNCTGGGTCCCACGTGGAGATAGTGM

Wl-1943b 1 65 TACAGGGCACCGNTGAGCATTCCAGATGACTCCAMGCCCCGGCTGGAGTAT

ATFCCAGTTTCACAGTGGGCACAGGAGTCAGATTAGGGCTMGTTGGGGGGACAGGATGCACAGCGT GTTGGCTCAGGATCTCTGGGAGGTGGCACCTGTGACCTGGGCTMNCATGCTACTTTCAGAGTCMGC AGCMGCCMTGGGTAGGGAMGACCAGC[C/T]CCTCTGMNCTGGGTCCCACGTGGAGATAGTGM

WI-1943 1 64 TACAGGGCACCGNTGAGCATTCCAGATGACTCCAMGCCCCGGCTGGAGTAT

CCAGGTGAGGCTGAMGMGGMGGAGGCMTTGCTGTTGGAGTGAGGGATTCTGGAGMGCACCCT GCAGAGCTTCATTCTGTTTTCAAMGTGTGCCATGCANGGTCNTCTGGGTTGTGAGCTCATNGCTGAG TTATCACAGCTCCTGATGACAGATCATGAAAMTAGGTACTTCCCMGCTCTGACTAGACCTTGGCA

WI-1960C 270 GTTGCMTTMATCCGTGGTGTCTGAAMCTTAAAMTGCACCTCCCMCTTT

CCAGGTGAGGCTGAMGMGGMGGAGGCMTTGCTGTTGGAGTGAGGGATTCTGGAGMGCACCCT GCAGAGCTTCATTCTGTTTTCAAMGTGTGCCATGCANGGTCNTCTGGGTTGTGAGCTCATNGCTGAG TTATCACAGCTCCTGATGACAGATCATGAAAMTAGGTACTTCCCMGCTCTGACTAGACCTTGGCA

Wl-1960b 270 GTTGCMTTAMTCCGTGGTGTCTGMMCTFAAMATGCACCTCCCMCTTT

CTGATGCCMGTGCAGCTTAGAGTNAGGMTCCAGAGAMGTNTTTGGATCTGGTMGTAGGAGTCA TTCTGGGCATTTCTTCATAGAGTNTTG I I I I lAGTCTCGTMTMTACTGTTGCCCTAGGMGGTTGTT

TTTCCTACTGCGTCTGTGAMGCCTTTCCCCATCGAGTGATACAGTACTTTCCAGTTATGGAGATTT[

WI-1977 203 /C]TMCMTCMACACTGGCTGAGGCTGTTGG

AMTTCTAGMGCCAGMGTCAGCTCACGATTTATAMGTTGMGTMATGCATTGTAGTTTCATGT TTTCTCTTAATTCTGCACAMACTAGCTAAAMTC[T/C]TTTMATCAGTTACCAGAGGCAATACCT GGGTTMTGTMGCACTCAMAGTTATGTAGAGTAGCTGTCTCTGAGTCAC I I I I I I CTACTCTCATT

WI-2012 1 02 | GGCTTCACCMTGCTTCCACTGGATC

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TTGGTTGGCATTTTAGCCTCATAACMCTATTTACMTCATAATTGTTACTCTTATTTTACMACMG MAMTGAGGCTTMCATCACACTTCTGCTTAGTCGCAGAGCCMGATTTGMCCCAGGMTCCATT CACCGGTAC[A/G]TGCTACCTGGGTAAMAATGTTTAATTAAMTCTATGGCATTAGATTTCAMGA

WI-4584 144 GTCCTMTGTGGTTTTGMMTAGGTGTGCTTTMTTTGTFTATCAGTATGC

TTTCTGCATTTGMTGTGTATGGTCAGACTTCAGAGGMCCCAGGMTCTCATTTATTCAGTACMTA TGGTGGCCAGGTGCTCAGGCCCTATTATCAGAGAGATCTCAGTTTMCTTTCCMTTCCACCATTTAC TGACCATATGACTTGGGGMCATTATCTCACCTATCTGAGTCTGTATCC[CtηCATCTTTAMTTGTA

WI-4639 1 85 AATTTTAAGGACACCTATCATAGTAATATTGTGAGGATAAAATGAAATAA

AMTGMTCCGCTFTAGAGCAMTACCAGTMGGGCTGGTGCAGGATGGTGGTGGCTGAGAGA[A/- ]GATTACTCATAAAAGCATATTAATTTTATAAATATGGAMATTFAACTAGATAATTAAATGTGAAT TGAGTTTGMGGTTGCATGAGAGTAGGGAGGAGGTAGTTTCTACTTATAGGGTTTATATMGTNTGCT

WI-5327 63 A TCMTAGMTGGCTCTTTCGGATGACAATGATGAACTGTTCTMGCAGACAG

GCTTTTGAGMTGMMGGGGAGCCTGGACCATTGCAGGGCTTCTTCATCTCTGATTATTTTGTGTAT TTATTGTTCACTTATTTAT[C/T]GTCTGTCTCCCCTTCTGGTATGCTFGTGTCATGAMCMT GMTTC CCCAGTGCCTGGCCCGATTCGTGGCTCCTAGAGGTGTCCAGAAAAAMGTTTCGGTGMTAGMTTG

WI-5390 87 ACGMTGGGTTCAGMTTGMACCTGTGMTCTATGGMGACAMCGAAT SI

CCTTGCCTGCTTTATGCATMTGAGMTAGAGTTGACTCTCCTGTCMGMATCMTFATTMGCAGT GCAAACATTATTTTAATTT[G/A]AAAGAAACTTGTTTCTGAAACTTTGTACTCTTGTAGTNAAATTG MTCTTTCCTTCTCAGCAGTTTCCATGGTCGTGMTCCACCCCATCTCTTTTCACCAGTAGCMGATT

Wl-5404b 87 GCTACTTATATGGAAGGGTTTTAGAGTTCATMCM

CCTTGCCTGCTTTATGCATMTGAGMTAGAGTTGACTCTCCTGTCMGMATCMTTATFMGCAGT GCAAACATTATTTTAATTT[G/A]AAAGAAACTTGTTTCTGAMCTTTGTACTCTTGTAGTNAAATTG MTCTTTCCTTCTCAGCAGTTTCCATGGTCGTGMTCCACCCCATCTCTTTTCACCAGTAGCMGATT

WI-5404 I 87 GCTACTTATATGGMGGGTTTTAGAGTTCATMCM

TAGGAMGGGGATGGTGATGGCCTCTGAGACATFTAMTCTATTCTTTCACCACTCACACTGCCGCCA TATCTCCTC[A/C]CCMCACCTCTGTTTTCTGACAGCCMGTTTCCATCAGTTGATATGGGACTATTT GTTGCAAMCMTTGTTMMGATTTGGCTGACTTTGGCTGMTTTGCTACMCTCCMMAGANTC

Wl-5545b 77 GAGATACACCATGMTTTTATTTTCATTTCA

TAGGAMGGGGATGGTGATGGCCTCTGAGACATTTAMTCTATTCTTTCACCACTCACACTGCCGCCA TATCTCCTC[A/C]CCMCACCTCTGTTTTCTGACAGCCMGTTTCCATCAGTTGATATGGGACTATTT GTTGCAAMCMTTGTTMMGATTTGGCTGACTTTGGCTGMTTTGCTACMCTCCAAAMGANTC

WI-5545 77 A' C GAGATACACCATGMTFTTATTTTCATTTCA

SI CΛ

TMTTGCACAACTTACATATCAGGGTTTCTGATTGAMGGMGAGMTATTCCTTTCTTTTAGTGATT GCTTAATATTMTTCATAATMGTGCACCATCTCTΓF/CJGCTCCTTATAAATGTGTTTAGMGMGG MATTGAGTGTTGGGMTTMGCMCCAGGAGACATTTTTATATACTCCTACAGTGGGGGMGACTT

WI-6244 1 03 CCTATTTTCTΓTCCCMGGATGGATACATTTCTAC

CTGGCCTTATMTCCMGTFTAGGATTMTCTTACCCCMCTTMTAGACTFCCAGACAGTTGCAGTT GTCTACMGATTTCCTCCTAGTAGGGCTTTGGGTGTTGGCACCGTTTGGCTCATTC[C/ΗACTCTCCCT GGGTCTTATTGACTTTCAGGGAGCCTAGMGAGCTGGACMMCCTGCTTCTTTGCAGAMGAGTCG

WI-6268 1 24 GGGTTCCAMGATTTCGTTACGAI M I M A

AGGTGCCATTTMTCCATTCAMTTTGGMGCTACATCTTCMGGGTCTGAGAGAGCTCACTCCCCCC ATATATTCCCCCTTFACATGTTTFCTFATMGACATACAGTTTAATCMTTAACAMCTMACAGCTT ATATACTGGCMTATATTACAGATGGGTTTATGTCAGAGTAATAGATCACATGAMTGGACCATGTG

Wi-6336b 234 GTACCCCAGTGCATTATGTCTTGGTAGAGCC[C/T]TGAGGACACTGACAGT

AGGTGCCATTTMTCCATTCAMTTTGGMGCTACATCTTCMGGGTCTGAGAGAGCTCACTCCCCCC ATATATTCCCCCTTTACATGTTTTCTTATMGACATACAGTTTMTCMTTAACAAACTAMCAGCTT ATATACTGGCAATATATTACAGATGGGTTTATGTCAGAGTAATAGATCACATGAAATGGACCATGTG

WI-6336 234 T GTACCCCAGTGCATTATGTCTTGGTAGAGCC[CtTTFGAGGACACTGACAGT

SI

TTGGATACAAAAATTCAGTTACACAATCAGTAGCATTCMMTTAGTTATGAGTATTTATACAATTA ON CAAAAATGGNTTCATGTrTTMCM[C/A]GTATTTTAMAGCTCAAACATTTTAAAACAGGCACAAT ATTCTMNGGCATATGCATTCACCATGGGCTTTTGMTGTCCTCACTCCCMCTTCACMTCAAMTC

WI-6381 92 TACAGANGCGGCAAMGATCAGAGTTCAG

GGTTGAGGCATTGGGAMGGCAGAMTTGAGGCAGTAGAMATGGACATTTTAGGAAMGAGMGT TCAGAGGCAAAGTCATGACAGACAGGAMTACMGGCTTAGGMGACAGTAGTCTCTGTGGTTGM ATTTTGGTGTCATMTMGMGTTTAGACTTTGGTGGTTGTAGTAGTTGTAGTAGTAGGTAGCGTT[C/

WI-6436 1 98 G]ATTGGGTGTATTCCACAGACMGGTGATGTTCTMGATTTGATATTTATTGT

GAGGCCTCTTTGCTTTTCCTCAGTCMGGCTGTATCCAGGGTTGATATCTAGCCTATATGCCATATGT GTATGGCTAGTGTTTGTTCTGATTGGTTGGTGCTCACACTGCCCAGATTGTTAMTATTTTGAAMTC GTATCTGGTTCTATTCATCTGCATTCTCTGATCTTATGTCTGGCTCTATT[C/ηATCCCTATTCTCTGA

WI-6449 1 86 TCTTATGTCAGACCTGMGTTCCTCTMI I I I I CTGTGGTGTATTTATA

GAGGCCTCTTTGCTTTTCCTCAGTCMGGCTGTATCCAGGGTTGATATCTAGCCTATATGCCATATGT GTATGGCTAGTGTTTGTTCTGATTGGTTGGTGCTCACACTGCCCAGATTGTTAMTATTTTGAAMTC

GTATCTGGTTCTATTCATCTGCATTCTCTGATCTTATGTCTGGCTCTATT[CA]ATCCCTATTCTCTGA

WI-6449 1 86 C T — TCTTATGTCAGACCTGMGTTCCTCTM I I I I I CTGTGGTGTATTTATA

GCTGGAGAGAAMGACCTCCAAMGMGAMCTMATCAGAGTCTCTTGAGCMGAGGMTTGMA AGAACA[T/C]TGAAAAMATTAAAGTAGAACTCAMGAGCCMAAAGTCCCCMTTGTGTCCATTA TMGMATATTFTGAATGGAMTCTTMGAATGATTTTATTGATCAGTTAMTGTTCTTCCTCTCCTC

WI-6463 72 CAGTCCCATTTATATGACATTCCGCATGCTG

MGCAGTAMTCTTCCATCATGCCATGGATGCCAGTGGGTAMTGTTATAGAMCTTCAGAGGANAC AGAGGCAM[C/T]GTTGGTTATAGCAGTCMCGACATCATCMTGMGACATGACTTGCTTAGAGCC MGMMAGTAGGATTTTGAMGGCACAGAGAAMGGGGTGTACTAGAGGAGMCTATGTMGCAG

Wl-6474b 76 T AGGTATAGAGGMCTMAGTATAAMGAGTGAGCCATMCTTAGGGTACCATAA

MGCAGTAMTCTTCCATCATGCCATGGATGCCAGTGGGTAMTGTTATAGAMCTTCAGAGGANAC AGAGGCAM[GT]GTTGGTTATAGCAGTCMCGACATCATCMTGMGACATGACTTGCTTAGAGCC MGAAAMGTAGGATTTTGAMGGCACAGAGAAMGGGGTGTACTAGAGGAGMCTATGTMGCAG

WI-6474 76 AGGTATAGAGGMCTMAGTATAAMGAGTGAGCCATMCTTAGGGTACCATM

GMCTCMTTMCTTTGCMCACTGAGAAMTCGGATTTGGAGATCTGCAMGCTGAGGTTGAGATT TTGGACCTTGGTGATCCAMTGGGGMTGCCACGCTTCGAGGCCTGTCTATATGCTTTATTTTTGTGA CACTGTCTATTTACCCTCCCCCMTAGTGGAGMTCAGAG[T/A]GCTCCTTGTCAGTGTTGCTACAGA

Wl-6478b 175 GMGATATACAGGATGGMGGACAGCTCCTCGTAGGACCTAGACACMCTG

GMCTCAATTMCTTTGCMCACTGAGAAMTCGGATFTGGAGATCTGCAMGCTGAGGTTGAGATT s

-4 TTGGACCTTGGTGATCCAMTGGGGMTGCCACGCTTCGAGGCCTGTCTATATGCTTTATΠTFGTGA CACTGTCTATTTACCCTCCCCCMTAGTGGAGMTCAGAGΓF/A]GCTCCTTGTCAGTGTTGCTACAGA

WI-6478 1 75 GAAGATATACAGGATGGMGGACAGCTCCTCGTAGGACCTAGACACMCTG

CACATTTTGMTGCMCTGAGAMNTGGTTΓTNTAGGCCTACCTTTTATTTMGAGTACATCTGGCTC CMTGTTACCCCAMCATGCAMACATMGGCMCMTTCTGATCATTTTATAGGNTCCCMGCCCA

TTAGCAATATCTTA[G/A]TCAAATTTTAMAAGAGMCAGGAAATMGGAAGGCCTMCAGAGGAG

WI-6559 149 TTAAATMTTGTGCAAMCTTATCAGTTCTTC

TTCTTTATTGGTCCTACCMTGTGACTCTTTACCCAGGCCCACTGTTCCTATGC[G/A]CACTGGCTTTG TAGGCATTCACATCATATGTCTGTGTCCTGAAMTCTCMTTMTTTCTCCTNCCTATFCCTTTTCCATl GCTCTGCCTCATTTNCTCAGAMTTGMGGCATTTGATTATNA I I I I I I I GTTTGGGTCTGTGTAMG

Wl-6564b 54 GTTCCTTGGCAGGAGMCATGCATATGACTTTAAMTMAGACCMCA

TFCTTTATTGGTCCTACCMTGTGACTCTrTACCCAGGCCCACTGTTCCTATGC[GyA]CACTGGCTTTG

TAGGCATTCACATCATATGTCTGTGTCCTGAMATCTCMTTMTTTCTCCTNCCTATTCC^' FCCATl

GCTCTGCCTCATTTNCTCAGAMTTGMGGCATTTGATTATNATTT1 GTTTGGGTCTGTGTAMG

WI-6564 54 G! GTTCCTTGGCAGGAGMCATGCATATGACTTTAAMTMAGACCMCA

SI

00

S>

VO

GCATGATTAMCCAGTGCAGAMMTACCMGTACATTGGGTGMCGATGAGCTAGCTGTTCTAGTA TTTGCTTTTTGTMTCCAGTTMGACCATCAGCATATACAACATCATCACTAACTCMCMTGTAGCT GCAGGGTMC[C/A]TGTGGATACCCTGTGTGCTCTACTNGCCTCCAMGGCATTCAGGGGATCATCA

Wl-6817 1 45 MGATGTTGGACACCTTGTGTTCAMTCTTGGTTCAGGTGCGGCCTGTGCAG

GATGGMAGCCATTTTA I I I I I CTCTMATTTTAAAATAGMGACTTTAATGGAAMCATTTAGTAC CATCATGTCACCCTGMTGCCAGCMTACCTCGACTTTTACACACGCAGGMGCCTAGTAAMGCCC CGTCAGTAGTACACATTTCTCTATGGTCCTTCMCAGTT TTTTGGCCAATTAATTAACCAAAAMMTTTTTCTGCTATTT

Wl-6819b 221 CTTTAGCAMCAGCMTMCT TTGTGTTTCCTATATGACACCTAATATCCAG

GATGGAAAGCCATTTTA I I I I I CTCTAAATTTTAAAATAGMGACTTTAATGGAAMCATTTAGTAC CATCATGTCACCCTGMTGCCAGCMTACCTCGACTTTTACACACGCAGGMGCCTAGTAMAGCCC CGTCAGTAGTACACATTTCTCTATGGTCCTTCMCAGTTTT[G/T]CATATACAMATTTTCTGCTATT

Wl-6819a 175 TTGCTTTAGCMACAGCMTMCTTTTGTGTTTCCTATATGACACCTMTAT

GCAAAMGCTTTATTGGCTCCMCMATTATCCCTTTTAAMCTCCTCTTCTTCTTCTGGTCTCAGTG GAACAACACATTTGMTTTCAGATTTGCAGTTTATAGCA I I I I I I I I CCCTAAGMCCATATAMTAC ATGCAAAACCTTGTACAT[A/G]GAGCTTAAATMTATCAAAATGCAAATATAGATTGGGTGCACTGT

Wl-6826b 1 54 TMGCTGMTTGCAMTTATGGCMCACACACTGGACTGGGGTATACGTTG

GCAAAMGCTTTATTGGCTCCMCAMTFATCCCTTTTAAAACTCCTCTTCTTCTTCTGGTCTCAGTG UI

© GAACAACACATTTGAATTTCAGATTTGCAGTTTATAGCA I I I I I I I I CCCTMGAACCATATAMTAC ATGCAAAACCTTGTACAT[A/G]GAGCTTAAATMTATCAAAATGCMATATAGATTGGGTGCACTGT

WI-6826 1 54 TMGCTGMTTGCAMTTATGGCMCACACACTGGACTGGGGTATACGTTG

AGTGCAMCTATTTTGMCAAMGTAMCTATGAGTCACAGCATTCAGCMGACATCAGACACGGA AGAGTGMCMTATTCACTMGTMAATACAGCAGATGAGATGTCTCTCACATGTA[T/C]ATTTMT TATFCATGC I I I I I CMTAGTCTCTTAGTCMCTTTCAGTGTMTTTCCACAMTATATAGCAGCTCA

Wl-6857a 1 22 MCACMATGCAGGAGCACMTGGCMAGTTTGGCMCTGTTTTGGGCTMTT

TTATAGMTACTTATGGGGCATACGNGTAMTGAACTGTCMCCTTMAATCTAMCMACAGCTTG TTTGTGGTTCGTCCTGMATCCTCCCTGCTCACAAMCAGCCAGCTACTNGGTTTTCTAAMGACGTA ATTTTGCAGGCAMCTTC[G/A]TAGAGCCATTCTGTGCAGMGMGGGMGGGAGMGCTGTTTGTT

WI-6865 1 53 G A TTACCTGTAGTATGMGATATTCTTTGCGCTGTTAGMCTGAGCTCATFM

ATTGAMACTGGTTAGCMCAGATAMTTACMTAGAGCCTGGATATAMMTGAGAGMGMTGC AGACTTA[C/T]MGCTTATAGAGAMGTCAAAMGGAGCMGTTTTTGMATCAGATTTTATGATAC GGAAAAAAMTTFCCTTFTTTTGCCMCAGGATTATTTCGMTAATAMTCTGCCAGTGCCMTCAG

WI-6909 73 C T AMCACCATTTCCACMTATTTGCATGCCCCTAGTTGCCTATTTTATACATATC

UI

ACTTCTAGTGCCTCTGTTACCACCACCTCTMTGCCTCTGGTCGCCGCACTTCTGATGTCCGTAGGCCT TMATCTGCCTGGCGTCCCCTCCCTCTGTCTTCAGCACCCAGAGGAGGAGAGAGCCGGCAGTTCCCTG CAGGAGAGAGGAGGGGCTGCTGGACCCMGGCTCAGTCCCTCTGCTCTCAGGACCCCCTGTCCTGACT

Wl-6996b 242 CTCTCCTGATGGTGGGCCCTCTGTGCTCTTCTCTTCqG/ηGTCGGATC

ACTTCTAGTGCCTCTGTTACCACCACCTCTMTGCCTCTGGTCGCCGCACTTCTGATGTCCGTAGGCCT TAMTCTGCCTGGCGTCCCCTCCCTCTGTCTTCAGCACCCAGAGGAGGAGAGAGCCGGCAGTTCCCTG CAGGAGAGAGGAGGGGCTGCTGGACCCMGGCTCAGTCCCTCTGCTCTCAGGACCCCCTGTCCTGACT

WI-6996 228 CTCTCCTGATGGTGGGCCCTCTG[T/G]GCTCTTCTCTTCCGGTCGGATC

TGGGGAGGACAGGGAGATGCTGCAGTTCCAAMGAGMGGTFTCTTCCAGAGTCATCTACCTGAGTC CTGMGCTCCCTGTCCTGAMGCCACAGACMTATGGTCCCAMT[G/A]CCCGACTGCACCTTCTGTG CTTCAGCTCTTCTFGACATCMGGCTCTTCCGTTCCACATCCACACAGCCMTCCMTTMTCMACC

Wl-7021 b 1 1 2 ACTGTTATTMCAGATAATAGCAACTTGGGAMTGCTTATGTTACAGGTTA

TGGGGAGGACAGGGAGATGCTGCAGTTCCAAMGAGMGGTTTCTTCCAGAGTCATCTACCTGAGTC CTGMGCTCCCTGTCCTGMAGCCACAGACMTATGGTCCC[A/G]MTGCCCGACTGCACCTTCTGTG CTTCAGCTCTTCTTGACATCMGGCTCTTCCGTTCCACATCCACACAGCCMTCCMTTMTCMACC

WI-7021 1 08 ACTGTTATTMCAGATAATAGCAACTFGGGAMTGCTTATGTTACAGGTTA

UI

GGCAGTAGGACCACCAGTGTGGGGTTCTGCTGGGACCTTGGAGAGCCTGCATCCCAGGATGCGGGTGG SI CCCTGCAGCCTCCTCCACCTCACCTCCATGACAGCGCTAMCGTTGGTGAfC/ηGGTTGGGAGCCTCT GGGGCTGTTGMGTCACCTTGTGTGTTCCMGTTTCCMACMCAGAMGTCATTCCTTCTTTTTAM

WI-7056C 1 1 8 ATGGTGCTTMGTTCCAGCAGATGCCACATMGGGGTTTGCCATTTGATA

GGCAGTAGGACCACCAGTGTGGGGTTCTGCTGGGACCTTGGAGAGCCTGCATCCCAGGATGCGGGTGG CCCTGCAGCCTCCTCCACCTCACCTCCATGACAGCGCTAMCGTTGGTGA[C/ηGGTTGGGAGCCTCT GGGGCTGTTGMGTCACCTTGTGTGTTCCMGπTCCMACMCAGAMGTCATTCCTTCTTTTTAM

Wl-7056b 1 1 8| ATGGTGCTTMGTTCCAGCAGATGCCACATMGGGGTTTGCCATTTGATA

MTTCGCTGMAAAGGMCTACCTATCCTTACATTTCACCTACTMTGTCTCTTCTMCATCTTAGAG GTCCATGGAGMGGCATATGGAGMCATGTTTTATACTGCTCTATAMTAGTATTCCMTCACTGTG CTTAATTTAAATAGCATT[A/C]TCTTATCATTFATCAGCCTTTTATGTATTTTCCAAGTAAAATATTA

Wl-7091 b 1 53 ACATATTATTTCATFGGTCTTC I I M I I ATCTGGTTCTATATGMTGCTAT

MTTCGCTGMMAGGMCTACCTATCCTTACATTTCACCTACTMTGTCTCTTCTMCATCTTAGAG GTCCATGGAGMGGCATATGGAGMCATGTTTTATACTGCTCTATAMTAGTATTCCMTCACTGTG CTTAATTTAAATAGCAT [A/C]TCTTATCATTTATCAGCCTTTTATGTATTTTCCMGTAAAATATTA

WI-7091 1 531 ACATATTATTTCATFGGTCTTC I I I I I I ATCTGGTTCTATATGMTGCTAT

TGTGMGCCACATTTTCCMCATGAGCCTCATGMGCCAACTMGTGTTATTGMCTGΓΓ/CIMTTC TCTCMTMCTCAGTGTAGCACTTTAMGTCTGMGGACAGCMCATGAAMGAGCATATCMTGTG

GTGGAGAMGGGMGGGGTTGGC I I I I I MTTTAT TTTTCTTCATCTTTTATMCMGMAGNNNNN

WI-7136 58 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTAGCTTTCTATATATG

GGGACGCCTGTTGTT1TGGCTCAATTTGGGTTTGTTGGTCACATGGAGCTCTTCCATTTCGTTTAGCTG MTMTGAGTTGTTCCTAGAGGAGACAGCCTGTCTCTCCTFGTTGCCCCCAMGCCCATGCCCTGCCG TGGTGGCAGCTGGGGCTGTGGATGGGAGGGGTCCCCMCATGGATGTGTTGCCCCTCCTCCGCATGCC

WI-7146C 21 0 MCGC[A/G]GTTCATGTACMGGCCCCTCTGCMCTGGAGAGAAMTTA

GGGACGCCTGTTGTTTTGGCTCMTTTGGGTTTGTTGGTCACATGGAGCTCTTCCATTTCGTTTAGCTG MTMTGAGTTGTTCCTAGAGGAGACAGCCTGTCTCTCCTTGTTGCCCCCAMGCCCATGCCCTGCCG TGGTGGCAGCTGGGGCTGTGGATGGGAGGGGTCCCCMCATGGATGTGTTGCCCCTCCTCCGCATGCC

Wl-7146b 21 0 MCGC[A G]GTTCATGTACMGGCCCCTCTGCMCTGGAGAGAAMTTA

GGGACGCCTGTTGTTTTGGCTCMTTTGGGTTTGTTGGTCACATGGAGCTCTTCCATTTCGTTTAGCTG MTMTGAGTTGTTCCTAGAGGAGACAGCCTGTCTCTCCTTGTTGCCCCCAMGCCCATGCCCTGCCG TGGTGGCAGCTGGGGCTGTGGATGGGAGGGGTCCCCMCATGGATGTGTTGCCCCTCCTCCGCAηGA

WI-7146 202 ICCMCGCAGTTCATGTACMGGCCCCTCTGCMCTGGAGAGAAMTTA

UI

ATATTACMCTTGC I I I I I AGCTGATCTTCCATCCTCMATGACTC I I I I I I CTTTATATGTTMCATA I TATAAMTGGCMCTGATAGTCMTTTTGAI I I I lATTCAGGMCTATCTGAMTCTGCTCAGAGCCT ATGTGCATAGATGAAACNNNNNNNtA/T]AAAAAAAGTTATTTAACAGTAATCTATTTACTAATTAT

WI-7153 1 61 AGTACCTATCTTTAMGTATAGTACATTTTACATATGTAAATGGTATGTTT

TAGMTAGATGCGGTCATATTCTTCTTTGGCTTCTGGTTCTTCCAGCCCTCATGGTTGGCATCACATAT GCCTGCATGCCATTMCACCAGCTGGCCCTACCCCTATMTGATCCTGTGTCCTAMTTMTATACAC CAGTGGTTCCTCCTCCCTGΓT/G]TAAAGACTMTGCTCAGATGCTGTTTACGGATATTTATATTCTAG

WI-7155 1 56 TCTCACTCTCTTGTCCCACCCTTCTTCTCTTCCCCATTCCCMCTCCAG

AGCTCCACCAGATGCAGATTTGTGTTΓTGTTTTCTTGTTATCACTGTCACACAGCTTATMCATGTAT

GCTTTTCAGMTACAGTTGTCTAGCCMGCCATCMGTGTCTGMATTCMTATTGGTTTATGCAMT

ACAGCAAACTTTTATTTAAGTAGAT[A/G]GGAGAATATGTTTAAAATATTAGGAATCCTAGACCATA

Wl-7169b 1 61 TTTCMGTCATCTTAGCAGCTAGGATTCTCAMTGGMGTGTTATATATA

CTCCTAGACTAGTGCTTTACCTTTATTMTGMCTGTGACAGGMGCCCMGGCAGTGTTCCTCACCA ATMCTTCAGAGMGTCAGTTGGAGAAMTGMGMMAGGCTGGCTGAAMTCACTATMCCATC AGTTACTGGTTTCAGTTGACAAMTATATMTGGTTTACTGCTGTCATTGTCCATGCCTA[C/T]AGAT

Wl-7175b 1 94 MTTTATTTTGTA I I I I I GMTMAMACATTTGTACATTCCTGATACTGGG

ui

4-.

UI CΛ

I -4

UI 00

U

VO

©

TGAMTCCTGGGTCTCTTGGCCTGTCCTGTAGCTGGTTTATTTTTTACTTTGCCCCCTCCCCAC I I I I I I TGAGATCCATCCTTTATCAAGAAG[T/A]CTGAAGCGACTATAMGGTTTTTGMTTCAGATTTAAM ACCMCTTATAMGCATΓGCMCMGGTTACCTCTATTTTGCCACMGCGTCTCGGGATTGTGTTTGA

WI-7388 94 CTTGTGTCTGTCCMGMCTTTTCCCCCAAAGATGTGTATAGTTATTGG

TTAGATTTTMTFGGCMCCAGCMCTCACTGCCACCATTCCACTGCAGATCTNCTATTCCTGG[A/G] GTTGATATGACMGGMACCCTATTGGMCCMGTCTTCAGATTGTNCCATGTGCAGACAGGCTCCT TGTCTGTAGGTGTAGTAGCATGTACACTGTACTGTTCACTGTMCATAGTTTGTNCTGGTATTTGTTA

WI-7438 64 TTGGAMTGMTATCGCTTCCACTGACTTTTACCA

CCATGATCCCCTCCTCTTGCCAMTGGAGGMGCCTGTGGATGGTACCMCAMCMGCCCCAMCC CAGTACAMCTGAGMTGAGAGMCCCTGATAGCACTGTCTGMTTGCCAGGAGCCTCCMGGCTM TCCTACCCCTGGATTTCT|T/C]TGTTGTTTAAGTTATTTCTAGCCACCACAMGAGGGTACTGCCCM

Wl-7454b 1 52 CAGACTCATCCTTAAAMATCCCATTTGTCTACTTCTCAMTG I I I I I GACA

CCATGATCCCCTCCTCTTGCCAMTGGAGGMGCCTGTGGATGGTACCMCAMCMGCCCCAMCC CAGTACAMCTGAGMTGAGAGMCCCTGATAGCACTGTCTGMTFGCCAGGAGCCTCCMGGCTM TCCTACCCCTGGATTTCT[T/C]TGTTGTTTMG^"FTATTTCTAGCCACCACAMGAGGGTACTGCCCM

WI-7454 1 52 CAGACTCATCCTTAAAAAATCCCATTTGTCTACTTCTCAMTG I I I I I GACA

AATTTGAAAATCTGAAAAAAAGTGCATAAGCAGAGAMTGACACTTATTCCAAATAAATAAATTGT 4i. SI CCA I I I I I CACTCAGTCCATCTTAACCATGTACAATGCACTAMTTACTATTFATMTTTCCTATGTA CMCAGAGCCACAGCACMGAGGGTGGGCATMGCAGTTGCCA[G/C]CCAGMGAGCTTTCACTCAT

Wl-7464c 1 77 GAMGMAGCCCTACAMTAGGCCCAGGAGMGCMCGTTCACCMCMTTAT

AATTTGAAMTCTGAMAAMGTGCATAAGCAGAGAMTGACACTTATTCCAAATAMTMATTGT CCA I I I I I CACTCAGTCCATCTTMCCATGTACAATGCACTAMTTACTATTTATMTTTCCTATGTA CMCAGAGCCACAGCACMGAGGGTGGGCATMG[C/A]AGTTGCCAGCCAGMGAGCTTTCACTCAT

Wl-7464b 1 68 GAMGMAGCCCTACAMTAGGCCCAGGAGMGCMCGTTCACCMCMTTAT

MTTTGMMTCTGAMMMGTGCATMGCAGAGAMTGACACTTATTCCAMTMATAMTTGT CCA I I I I I CACTCAGTCCATCTTMCCATGTACAATG[C/A]ACTAAATTACTATTTATMTTTCCTAT GTACMCAGAGCCACAGCACMGAGGGTGGGCATMGCAGTTGCCAGCCAGMGAGCTTTCACTCAT

Wl-7464a 1 03 GAMGMAGCCCTACAMTAGGCCCAGGAGMGCAACGTTCACCMCMTTAT

CMTTCTCMTCCMCCTAGTCTGTNTGCCTAMCCATTCCAGACAMCTTCCACTTCGMGGTTTTA MTGCATMGTCAGATAGCMTCCTTCAGTTGCCCCAGAGGCACATCACGTTCTTTGMTGCTTCA[T

/G]TATAGTCCTCTTCATTTAGCMTCAGTGAGGCMTACACTGGCATCATGATCCCI I I I I I IAGGA

Wl-7499b 1 34 G^! ACTCTGTACAAMTTCCCTTTGMMTATAMTTTTGGAAATGAGTGATGA

CMTTCTCAATCCMCCTAGTCTGTNTGCCTM[A/G]CCATTCCAGACAMCTTCCACTTCGAAGGTT TTAMTGCATMGTCAGATAGCAATCCTTCAGTTGCCCCAGAGGCACATCACGTTCTTFGMTGCTTC ATTATAGTCCTCTTCATTTAGCMTCAGTGAGGCMTACACTGGCATCATGATCCCTTTTTTTAGGM

Wl-7499a 33 CTCTGTACAAMTTCCCTTTGAMATATAMTTTTGGMATGAGTGATGA

TGGGMTAGTMGAGAMGATGGGAAAGGTGACCAMAACMTATAGAGGCAGAGGCCMGTGMT GCATCCCAGCAGCAGACCACTTNAAMGTAGTCCTGGTGCTGATFGCCTAGC[A/C]GGAGAGTTGAG TGCCACAGGTAAGAATGAGTGMGAGGAAAAMTCATGATGTCATGTATGCAGTMTTACTATGTCA

Wl-7506b 1 1 8 GMGMMTATTTTAAMTATTGGACCACTCTTGTTCTACCATCCCTACCCACT

TGGGMTAGTMGAGAMGATGGGAMGGTGACCAMMCMTATAGAGGCAGAGGCCMGTGMT GCATCCCAGCAGCAGACCACTTNAAMGTAGTCCTGGTGCTGATTGCCTAGC[A/C]GGAGAGTTGAG TGCCACAGGTMGMTGAGTGMGAGGAAMMTCATGATGTCATGTATGCAGTMTTACTATGTCA

WI-7506 1 1 8 GMGAAMTATTTTAAMTATTGGACCACTCTTGTTCTACCATCCCTACCCACT

TGTGMTTCTTAGCTCTGGMGGTGTTTATGCCTTTGCGGGTTTCTTGATGTGTTCGCAGTGTCACCCA AGAGTCAGMCTGTACACATCCCAAMTTTGGTGGCCGTGGMCACATTCCCGGTGATAGMTTGCT AMTFGT[CAF]GTGAAATAGGTTAGM I I I I I CTTTAAATTATGGTTTTCTTATTCGTGAAAATTCGG

Wl-7534b 1 43 AGAGTGCTGCTAAMπGGATTGGTGTGATCTTTTTGGTAGTTGTMTTT

TGTGMTTCTTAGCTCTGGMGGTGTTTATGCCTTTGCGGGTTTCTTGATGTGTTCGCAGTGTCACCCA AGAGTCAGMCTGTACACATCCCAAMTTTGGTGGCCGTGGMCACATFCCCGGTGATAGMTTGCΓΓ /C]MATTGTCGTGAAATAGGTTAGM I I I I I CTTTAMTTATGGTTTTCTTATTCGTGAAAATTCGG

WI-7534 1 35 AGAGTGCTGCTAAMTTGGATTGGTGTGATCTTTTTGGTAGTTGTMTTT

GGGMAGMTAAMTFAGCTTGAGCMCCTGGCTMGATAGAGGGGCTCTGGGAGACTTTGMGACC AGTCCTGTTTGCAGGGMGCCCCACTTGMGGMGMGTCTMGAGTGMGTAGGTGTGACTTGMC TAGATTGCATGCTTCCTCCTTTGCTCTT[G/A]GGMGACCAGCTTTGCAGTGACAGCTTGAGTGGGTT

Wl-7543b 1 62 CTCTGCAGCCCTCAGATFATTTTTCCTCTGGCTCCTTGGATGTAGTCAGTTA

GGGAMGMTMMTTAGCTTGAGCMCCTGGCTMGATAGAGGGGCTCTGGGAGACTTTGMGACC AGTCCTGTTTGCAGGGMGCCCCACTTGMGGMGMGTCTMGAGTGMGTAGGTGTGACTTGMC TAGATTGCATGCTTCCTCCTTTGCTCTr[G/A]GGMGACCAGCTrTGCAGTGACAGCTTGAGTGGGTT

WI-7543 M 62 I GJ A CTCTGCAGCCCTCAGATTATTTTTCCTCTGGCTCCTTGGATGTAGTCAGTTA

GGTGATCMGATCTGTTCCACAGGGCTMTGCCACCATCTCCCCTCAMATTTGTAGAGGtT/C]TCTA MMGAMGTGGTATGTTGTGTGATGATCAGCACTMGTCCTGCATTCCTGTTAMGCCACTTGGGTC ATMGMGGGMGTAAAAMTGAAGTCTGACTAGAMTTCTATTGCAGAGGCCMGTACATTTAGT

WI-7555C 60 T Ci ATGGCATTGAGTTGTGATATAGTTTTCATTTGATGTGCATTTTGMTTTCAG

4i.

CΛ

MCCATGTTCCCTTCTTCTTAGCACCACAMTMTCAAMCCCMCATMGTGTΓTGCTTTCCTTTM AMTATGCATCAMTCGTCTCTCATTACTTTTCTCTGAGGGTΠTAGTA[A/G]ACAGTAGGAGTTMT AMGMGTFCATΠTGGTTTACACGTAGGAMGMGAGMGCATCAMGTGGAGATATGTTMCTAT

W1-7577J 1 1 7 TGTATMTGTGGCCTGTTATACATGACACTCTTCTGMTTGACTGTATTTC

MCCATGTTCCCTTCTTCTTAGCACCACAMTMTCMMCCCAACATMGTGTTTGCTTTCCTTTM AMTATGCA[T/C]CAAATCGTCTCTCATTACTTTTCTCTGAGGGTTTTAGTAMCAGTAGGAGTTMT MAGAAGTTCATTTTGGTTTACACGTAGGAMGAAGAGMGCATCAMGTGGAGATATGTTMCTAT

WI-7577J 77 TGTATAATGTGGCCTGTTATACATGACACTCTTCTGAATTGACTGTATTTC

MCCATGTTCCCTTCTTCTTAGCACCACAMTMTCMMCCCMCATM[G/C]TGTTTGCTTTCCTT TMMATATGCATCAMTCGTCTCTCATTACTTTTCTCTGAGGGTTTTAGTAMCAGTAGGAGTTMT AMGMGTTCATTTTGGTTTACACGTAGGAMGMGAGMGCATCAMGTGGAGATATGTTMCTAT

Wl-7577 50 TGTATMTGTGGCCTGTTATACATGACACTCTTCTGMTTGACTGTATTTC

MCCATGTTCCCTTCTTCTTAGCACCACAMTMTCAAMCCCMCATAAGTGTTTGCTTTCCTTTM MATATGCATCAAATCGTCTCTCATTACTTTTCTCTGAGGGTTTTAGTAMCAGTAGGAGTTMTM AGMGTTCATTTTGGTTTACAC[G/A]TAGGAMGMGAGMGCATCMAGTGGAGATATGTTMCT

Wl-7577g 1 57 ATTGTATMTGTGGCCTGTTATACATGACACTCTTCTGMTTGACTGTATTTC

MCCATGTTCCCTTCTTCTTAGCACCACAAATAATCAAAACCCAACAT[A/G]AGTGTTTGCTTTCCTT

ON

TMAAATATGCATCAMTCGTCTCTCATTACTTTTCTCTGAGGGTTT FAGTAMCAGTAGGAGTTMT

AMGMGTTCAT GGTTTACACGTAGGAMGMGAGMGCATCAMGTGGAGATATGTTMCTAT

Wl-7577f 48 G TGTATMTGTGGCCTGTTATACATGACACTCTTCTGMTTGACTGTATTTC

AACCATGTTCCCTTCTTCTTAGCACCACAMTMTCMAACCCMCATMGTGTTTGCTTTCCTTTM MATATGCATCAAATC[G/A]TCTCTCATTACTTTTCTCTGAGGGTTTTAGTAMCAGTAGGAGTTMT MAGMGTTCATTTTGGTTTACACGTAGGAMGMGAGMGCATCAMGTGGAGATATGTTMCTAT

Wl-7577e 84 TGTATMTGTGGCCTGTTATACATGACACTCTTCTGMTTGACTGTATTTC

MCCATGTTCCCTTCTTCTTAGCACCACAMTMTCMMCCCMCATMGTGTTTGCTTTCCTTTM MATATGCATCMATCGTCTCTCAT[T/C]ACTTTTCTCTGAGGGTTTTAGTAMCAGTAGGAGTTAAT AMGMGTTCATTTTGGTTTACACGTAGGAMGMGAGMGCATCAMGTGGAGATATGTTMCTAT

Wl-7577d 93 TGTATMTGTGGCCTGTTATACATGACACTCTTCTGMTTGACTGTATTTC

MCCATGTTCCCTTCTTCTTAGCACCACAMTMTCMMCCCMCATMGTGTTTGCTTTCCTTTM MATATGCATCAMTCGTCTCTCATTACTTTTCTCTGAGGGTTTTAGTAMCAGTAGGAGTTMTM AGMGTTCATTTTGGTTTA[C/A]ACGTAGGAMGMGAGMGCATCAMGTGGAGATATGTTMCT

WI-7577C I 1 54 ' C' A ATTGTATMTGTGGCCTGTTATACATGACACTCTTCTGMTTGACTGTATTTC

-4

4λ

00

÷-

VO

TTAMTGAGTGTGTTTGTCACCGTTGGGGATTGGGGMGACTGTGGCTGCTGGCACTTGGAGCCMGG GTTCAGAGACTCAGGGCCCCAGCACTAMGCAGTGGACCCCAGGAGTCCCTGGTMTMGTACTGTG TACAGMTTCTGCTACCTCACTGGGGTCCTGGGGCCTCGGAGCCTCATCCGAGGCAGGGTCAGGAGAG

Wl-7743d 275 T GGGCAGMCAGCCGCTCCTGTCTGCCAGCCAGCAGCCAGCTCTCAGCCMCG

TTAMTGAGTGTGTTTGTCACCGTTGGGGATTGGGGMGACTGTGGCTGCTGGCACTTGGAGCCMGG GTTCAGAGACTCAGGGCCCCAGCACTAMGCAGTGGAC[C/A]CCAGGAGTCCCTGGTMTMGTACT GTGTACAGMTTCTGCTACCTCACTGGGGTCCTGGGGCCTCGGAGCCTCATCCGAGGCAGGGTCAGGA

WI-7743Θ 1 06 GAGGGGCAGMCAGCCGCTCCTGTCTGCCAGCCAGCAGCCAGCTCTCAGCC

TTAMTGAGTGTGTTTGTCACCGTTGGGGATTGGGGMGACTGTGGCTGCTGGCACTTGGAGCCMGG GTTCAGAGACTCAGGGCCCCAGCACTAMGCAGTGGACCCCAGGAGTCCCTGGTMTMGTACTGTG TACAGAATFCTGCTACCTCACTGGGGTCCTGGGGCCTCGGAGCCTCATCCGAGGCAGGGTCAGGAGAG

Wl-7743d 275 | T GGGCAGMCAGCCGCTCCTGTCTGCCAGCCAGCAGCCAGCTCTCAGCCMCG

TTMATGAGTGTGTTTGTCACCGTTGGGGATTGGGGMGACTGTGGCTGCTGGCACTTGGAGCCMGG GTTCAGAGACTCAGGGCCCCAGCACTAMGCAGTGGAC[C/A]CCAGGAGTCCCTGGTMTMGTACT GTGTACAGMTTCTGCTACCTCACTGGGGTCCTGGGGCCTCGGAGCCTCATCCGAGGCAGGGTCAGGA

WI-7743C 1 06 GAGGGGCAGMCAGCCGCTCCTGTCTGCCAGCCAGCAGCCAGCTCTCAGCC

TTAMTGAGTGTGTFTGTCACCGTTGGGGATTGGGGMGACTGTGGCTGCTGGCACTTGGAGCCMGG GTTCAGAGACTCAGGGCCCCAGCACTAMGCAGTGGACCCCAGGAGTCCCTGGTMTMGTACTGTG TACAGMTTCTGCTACCTCACTGGGGTCCTGGGGCCTCGGAGCCTCATCCGAGGCAGGGTCAGGAGAG

Wl-7743b 275 GGGCAGMCAGCCGCTCCTGTCTGCCAGCCAGCAGCCAGCTCTCAGCCMCG

TTAMTGAGTGTGTTTGTCACCGTTGGGGATTGGGGMGACTGTGGCTGCTGGCACTTGGAGCCMGG GTTCAGAGACTCAGGGCCCCAGCACTAMGCAGTGGAC[C/A]CCAGGAGTCCCTGGTMTMGTACT GTGTACAGMTTCTGCTACCTCACTGGGGTCCTGGGGCCTCGGAGCCTCATCCGAGGCAGGGTCAGGA

Wl-7743 1 06 GAGGGGCAGMCAGCCGCTCCTGTCTGCCAGCCAGCAGCCAGCTCTCAGCC

TTAMTGAGTGTGTTTGTCACCGTTGGGGATTGGGGMGACTGTGGCTGCTGGCACTTGGAGCCMGG GTTCAGAGACTCAGGGCCCCAGCACTAMGCAGTGGACCCCAGGAGTCCCTGGTMTMGTACTGTG TACAGA TTCTGCTACCTCACTGGGGTCCTGGGGCCTCGGAGCCTCATCCGAGGCAGGGTCAGGAGAG

WI-7743 275 GGGCAGMCAGCCGCTCCTGTCTGCCAGCCAGCAGCCAGCTCTCAGCCMCG

TGACATTTATTCAMGTTMMGCAMCACTTACAGMTTATGAAGAGGTATCTGTTTAACATTTCC TCAGTCMGTTCAGAGTCTTCAGAGACTTCGTMTTMAGGMCAGAGTGAGAGACATCATCMGTG GAGAGAAATCtA/G]TAGTTTAAACTGCATTATAAATTTTATAACAGAATTAAAGTAGATTTTAAAA

WI-7758 1 441 GATMMTGTGTMTTTTGTTTATATTTTCCCATTTGGACTGTMCTGACTGCC

ACAGGGCCTTTGGCAGGTGCAGCCCCCACTGCCTTTGACCTGCCTCCCTTCATGCATGGAMTTCCCT TCATCTGGMCCATCAGAMCACCCTCACACTGGGACTTGCAAAMGGGTCAGTATGG[G/C]TTAGG GMMCATTCCATCCTFGAGTCAMAMTCTCMTTCTTCCCTATCTTTGCCACCCTCATGCTGTGTG

Wl-7765b 1 26 ACTCAMCCAMTCACTGMCTTTGCTGAGCCTGTAMATAAMGGTCGGA

TTMTTTACTGATTCCAGCMGACCAMTCATTGTATCAGATTΛ I I I I I MGTTTTATCCGTAGTTT GATAAMGATTTTCCTATTCCTTGGTTCTGTCAGAGMCCTMTMGTGCTACTTTGCCATTMGGCA

GACTAGGGTTCATGTC I I I I LACCCTTTNNNNNNNNNTTGTAAMGTCTAGTTACCTACTTTTTCTTT

Wl-7773b 237 G GATTTFCGACGTTTGACTAGCCATCTCMGCM[C/G]TTTCGACGTTTGA

TGCMCCTCTTTTCGTGATGGGCAGCCTGCTGGTCAGCACTCCAGTAGCGAGAGACGGCACCCAGMT CAGATCCCAGCTTCGGCATTTGATCAGACCAMCAGTGCTGTTΓCCCGGGGAGGAMCACTTTTTTM TTACCCTTTTGCAGGCACCACCTTTAATCTGTTTTT/C]ATACCTTGCTTATTAMTGAGCGACTTMA

Wl-7774b 1 70 ATGATTGAAMTMTGCTGTCCTTTAGTAGCMGTAAMTGTGTCTTGCT

GCAGAGACCTTCCMGGACATATTGCAGGATTCTGTMTAGTGMCATATGGAMGTATTAGAMTA TTTATTGTCTGTAAATACTGTAMTGCATTGGMTAAMCTGTCTCCCCCATTGCTCTATGAMCTGC ACATTGGTCATTGTGMTANNNNNNNNNNNGCCMGGCTMTCCMTTATTATTATCACATTTACCA

WI-7785C 1 65 TMTTTA^'ΓΓTTGTCCATTGATGTATTTATTTTGTAAATGTATCTTGGTGCTGC

GCAGAGACCTTCCMGGACATATTGCAGGATTCTGTMTAGTGMCATATGGAMGTATTAGAMTA CΛ I TTTATTGTCTGTAAATACTGTAMTGCATTGGMTAAMCTGTCTCCCCCATTGCTCTATGAMCTGC ACATTGGTCATTGTGMTANNNNNNNNNNNGCCAAGGCTMTCCAATTATTATTATCACATTTACCA

Wl-7785b 1 65 TAATTIATTTTGTCCATTGATGTATTTATTTTGTAMTGTATCTTGGTGCTGC

GCAGAGACCTTCCMGGACATATTGCAGGATTCTGTMTAGTGMCATATGGAMGTATTAGAMTA TTTATTGTCTGTAMTACTGTAMTGCATTGGMTMMCTGTCTCCCCCATTGCTCTATGAMCTGC ACATTGGTCATTGTGMTANN[-

/T]NNNNNNNNGCCAAGGCTMTCCMTTA^'TTATTATCACATTTACCATMTTTATTTTGTCCATTGA

WI-7785 1 56 TGTATTTAJTTTGTAMTGTATCTTGGTG __

TCTCCCCCTCATCCMCTCCGAMGTCTGMTCFCCCMGGAGGGCACCATCTTACAGAGACTCTCCC TGACGGTGGMTTTM[G/A]TTTAGGGTCCCTAAMGCATTTGACACACAGTTGTTGMTGACTGAC CCAAMTGTGMTGMGCTMTGTGMTGTGAGTGMGCTCCCTTCAGGCCCGCTGCCCTAGGATAT

WI-7789C 84 GCCCTCCTGGTGACTCGGGGGCTGTCTCAGACGACTAGCCCAGGACCCATCT _

TCTCCCCCTCATCCMCTCCGMAGTCTGMTCTCCCMGGAGGGCACCATCTTACAGAGACTCTCCC TGACGGTGGMTTTM[G/A]TTTAGGGTCCCTAAMGCATTTGACACACAGTTGTTGMTGACTGAC CCAAMTGTGMTGMGCTMTGTGMTGTGAGTGMGCTCCCTTCAGGCCCGCTGCCCTAGGATAT

Wl-7789b 84 ' G Ai — GCCCTCCTGGTGACTCGGGGGCTGTCTCAGACGACTAGCCCAGGACCCATCT

CΛ

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GCAGGAAATAGTCACTCATCCCACTCCACATMGGGGTTTAGTMGAGMGTCTGTCTGTCTGATGA TGGATAGGGGGCAMTC I I I I I CCCCTTTCTGTTMTAGTCATCACATTTCTATGCCAMCAGGMCG

ATCCATMCTTTAGT[CT]TTAATGTACACATTGCATTTTGATAMATTMTTTTGTTGTTTCCTTTG

Wl-7830d 1 50 T AGGTTGATCGTTGTGTTGTTRTGCTGCACTTTTTACI I I I I IGCGTGTGGA

GCAGGAMTAGTCACTCATCCCACTCCACATMGGGGTTTAGTMGAGMGTCT[G A]TCTGTCTGA TGATGGATAGGGGGCAMTCI I I I I CCCCTTTCTGTTMTAGTCATCACATTTCTATGCCAMCAGGA ACGATCCATMCTTFAGTCTTAATGTACACATTGCATTFTGATAAMTΓMTTTTGTTGTTTCCTTTG

WI-7830C 54 AGGTTGATCGTTGTGTTGTTTFGCTGCACTTTTTAC I I I I I I GCGTGTGGA

GCAGGAMTAGTCACTCATCCCACTCCACATMGGGGTTTAGTMGAGMGTCTGTCTGTCTGATGA TGGATAGGGGGCAMTC I I I I I CCCCTTTCTGTTMTAGTCATCACATTTCTATGCCAMCAGGMC[ G/A]ATCCATMCTTTAGTCTΓAATGTACACATTGCATTFTGATAMATTMTTTTGTTGTTTCCTTTG

Wl-7830b 1 34 AGGTTGATCGTTGTGTTGTTFTGCTGCACTTTTTAC I I I I I I GCGTGTGGA

GCAGGAAATAGTCACTCATCCCACTCCACATMGGGGTTTAGTA[A/G]GAGMGTCTGTCTGTCTGA

TGATGGATAGGGGGCAMTCI I I FT CCCCTTTCTGTTMTAGTCATCACATTTCTATGCCAMCAGGA ACGATCCATMCTTTAGTCTTAATGTACACATTGCATTTTGATAAMTTMTTTΓGTTGTTFCCTΓTG

Wl-7830 44 AGGTTGATCGTTGTGTTGTTTTGCTGCACTTΓTTACTTTTTTGCGTGTGGA

CCACTTCCTATCTGA I I I I I CCCAGFC/ΗAAATGAGGCAGGCAATTCTAGTCTTCCACAAAACATCTA 4i. GCCATCTAAMTGGAGAGATGAATCATTCTACCTATACAMCMGCTAGCTATTAGAGGGTGGTTGG GGTATGCTACTCATMGATTTCAGGGTGTCTTCCMCTGMATCTCMTGTTCTCAGTACGAAAMC

Wl-7865e 25 CTGAAATCACATGCCTATGTAAGGAMGTGCTATTCACCCAGTAMCCCAM

CCACTTCCTATCTGATTTTFCCCAGCAMTGAGGCAGGCMTTCTAGTCTTCCACAAMCATCTAGCC ATCTAAMTGGAGAGATGAATCATTCTACCTATACAMCMGCTAGCTATFAGAGGGTGGTTGGGGT ATGCTACTCATAAGATTTCAGGGTGTCTTCCMCTGAAATCTCMTGTTCTCAGTA[C/ηGAAMAC

Wl-7865d 1 91 CTGAMTCACATGCCTATGTMGGMAGTGCTATTCACCCAGTAMCCCMA

CCACTTCCTATCTGATTTrTCCCAG[CtηAAATGAGGCAGGCMTTCTAGTCTTCCACAAAACATCTA GCCATCTAAMTGGAGAGATGMTCATTCTACCTATACAMCMGCTAGCTATTAGAGGGTGGTTGG GGTATGCTACTCATMGATTTCAGGGTGTCTTCCMCTGMATCTCMTGTTCTCAGTACGAAAMC

WI-7865C 25 l C CTGAMTCACATGCCTATGTMGGMAGTGCTATTCACCCAGTAMCCCMA

CCACTTCCTATCTGATTTTTCCCAGCAMTGAGGCAGGCMTTCTAGTCTTCCACAAMCATCTAGCC ATCTAAMTGGAGAGATGMTCATTCTACCTATACAMCMGCTAGCTATTAGAGGGTGGTTGGGGT ATGCTACTCATAAGATTTCAGGGTGTCTTCCMCTGMATCTCMTGTTCTCAGTA[C/T]GAAMAC

Wl-7865b 1 91 CT CTGAMTCACATGCCTATGTMGGMAGTGCTATTCACCCAGTAMCCCAM

GCTCACTGTGACCCATCCTTACTCTACTTGGCCAGGCCACAGTAAMCMGTGACCTTCAGAGCAGCT GCCACMCTGGCCATGCCCTGCCATTGAMCAGTGATTMGTTTGATCMGCCATGGTGA[C/T]ACA AAMTGCATTGATCATGMTAGGAGCCCATGCTAGMGTACATTCTCTCAGATTTGMCCAGTGAM

Wl-7900d 1 28 T TATGATGTATTTCTGAGCTAAAACTCAACTAΓAGAAGACATTAAAAGAAATC

GCTCACTGTGACCCATCCTTACTCTACTTGGCCAGGCCACAGTAAMCMGTGACCTTCAGAGCAGCT GCCACMCTGGCCATG[CTΗCCTGCCATTGAMCAGTGATTMGTTTGATCMGCCATGGTGACACA AAAATGCATTGATCATGMTAGGAGCCCATGCTAGMGTACATFCTCTCAGATTTGMCCAGTGAM

Wl-7900e 84 TATGATGTATTFCTGAGCTAAMCTCAACTATAGMGACATTAAMGAAATC

GCTCACTGTGACCCATCCTTACTCTACTTGGCCAGGCCACAGTAAMCMGTGACCTTCAGAGCAGCT GCCACMCTGGCCATGCCCTGCCATTGAMCAGTGATTMGTTTGATCMGCCATGGTGA[C/ΗACA AAMTGCATTGATCATGMTAGGAGCCCATGCTAGMGTACATTCTCTCAGATTTGMCCAGTGAM

Wl-7900d 1 28 TATGATGTATTTCTGAGCTAAMCTCMCTATAGMGACATTAAAAGAAATC

GCTCACTGTGACCCATCCTTACTCTACTTGGCCAGGCCACAGTAAMCMGTGACCTTCAGAGCAGCT GCCACMCTGGCCATG[CT CCTGCCATTGAMCAGTGATTMGTTTGATCAAGCCATGGTGACACA AAMTGCATTGATCATGMTAGGAGCCCATGCTAGMGTACATFCTCTCAGATTTGMCCAGTGAM

WI-7900C 84 TATGATGTATTTCTGAGCTAAMCTCMCTATAGMGACATTAMAGAAATC

GCTCACTGTGACCCATCCTTACTCTACTTGGCCAGGCCACAGTAAMCMGTGACCTTCAGAGCAGCT GCCACMCTGGCCATGCCCTGCCATTGAMCAGTGATTMGTTTGATCMGCCATGGTGA[C/T]ACA AAAATGCATTGATCATGMTAGGAGCCCATGCTAGMGTACATTCTCTCAGATTTGMCCAGTGAM

Wl-7900b 1 28 T TATGATGTATTTCTGAGCTAAAACTCAACTATAGAAGACATTMAAGAAATC

GCTCACTGTGACCCATCCTTACTCTACTTGGCCAGGCCACAGTAAMCMGTGACCTTCAGAGCAGCT GCCACMCTGGCCATG[CTΗCCTGCCATTGAMCAGTGATTMGTTTGATCMGCCATGGTGACACA AAMTGCATTGATCATGMTAGGAGCCCATGCTAGMGTACATTCTCTCAGATTTGMCCAGTGAM

WI-7900 84 TATGATGTATTTCTGAGCTAAAACTCAACTATAGAAGACATTAAAAGAAATC

AGACTTAGGTACAATTGCTCCCCTTTTTATATA[C/T]AGACACACACAGGACACATATATTAMCAG ATTGTTTCATCATTGCATCTATTTTCCATATAGTCATCMGAGACCATTFTATAAMCATGGTMGAC CCTTTTTAAMCAMCTCCAGGCCCTTGGTTGCGGGTCGCTGGGTTATTGGGGCAGCGCCGTGGTCGT

WI-7901 C 33 CAC I CAGTCGCTCTGCATGCTCTCTGTCATACAGACAGGTMCCTAGTFCT

AGACTTAGGTACAATTGCTCCCCTTTTTATATA[C/T]AGACACACACAGGACACATATATFAMCAG ATTGTTTCATCATTGCATCTATTTTCCATATAGTCATCAAGAGACCATTTTATAAMCATGGTMGAC CCTTTTFAAMCAMCTCCAGGCCCTTGGTTGCGGGTCGCTGGGTTATTGGGGCAGCGCCGTGGTCGT

Wl-7901 b 33 CACTCAGTCGCTCTGCATGCTCTCTGTCATACAGACAGGTMCCTAGTTCT

CΛ

VO

ACMTCTCAGMGGACTGTGCMGTCMTGAGTCGCTTGTGMTTCTCATCTGGAM[C/T]GATCCC ACGTCTTAGMCCTTCACCACMGGAG I I I I I CTTGTAGTGATTCTCAMGTCTTGGTAGGCATTCGA ACTGGTCCTTTCACTTTGAGATTCTTTTCTTTTGCGCCTCTTATCMGTCAGCACACACCTTTTCCMG

Wl-8021 b 57 GATTTTACGTTGCGGCTTGTTAGGGGTGATTCGMTTCGGTGMTTGCCA

ACMTCTCAGMGGACTGTGCMGTCMTGAGTCGCTTGTGMTTCTCATCTGGAM[C/TJGATCCC ACGTCTTAGMCCTTCACCACMGGAG I I MTCTTGTAGTGATTCTCAMGTCTTGGTAGGCATTCGA ACTGGTCCTTTCACTTΓGAGATTCTTTTCTTTTGCGCCTCTTATCMGTCAGCACACACCTTTTCCMG

WI-8021 57 GAT ΓACGTTGCGGCTTGTTAGGGGTGATTCGMTTCGGTGMTTGCCA

CTGAAMTTTACTATGCTCTCCACMCMGAGCTCCCATTTTCCACAGACACAGTCMTGTCAGTCA GCTTGTATTCAGGAGGACAGGGCAGAGGGATCCCAGTGGCACTTCCCATGGGMGACAGMGAGAGT GGGCCCCAGAGATGGMGGACCCCAGTGTCATCACCAMCMCCATTTCAGCCGCTCTAGCCTCTM

WI-8024C 206 TTCCC[A/G]CTCTAGMCAGCTGGCCCTGGTCGTCAGTACACMGGAMGAGC

CTGAAAATTTACTATGCTCTCCACAACMGAGCTCCCATTTTCCACAGACACAGTCMTGTCAGTCA GCTTGTATFCAGGAGGACAGGGCAGAGGGATCCCAGTGGCACTTCCCATGGGMGACAGMGAGAGT GGGCCCCAGAGATGGMGGACCCCAGTGTCATCACCAMCMCCATTTCAGCCGCTCTAGCCTCTM

Wl-8024b 206 TTCCC[A/G]CTCTAGMCAGCTGGCCCTGGTCGTCAGTACACMGGMAGAGC

GMTGAGCCTTCCTAGCGCCGAGGGACCTGCTGCTGTTGTTGGCCTGCACATGCATTCTATGGMTGC TTTTTGGCCMGCGGGGGCACTGAGGACTMGCTCTGANNNNNNNNNATCTCGCCCAMCTCCTTTCT MGGAGTCTGGGGTGTCATGCCCTACAMCC[A/G]TAAATTCTCATCAGATGGATTTTATTTMCGTT

WI-8077 1 67 GTGTATTGTGACTTACTTTCCAATCTGACTCTGGCATMCMGGGMAAA

TCTAGGTTTMTCMAGCMTTTGCANTTTGGATTTTGGMTGACCACTCCCTTGCTMGGMGCTAT GTACTTCATGCTGTGGAMCTGGCAMTACAGMTGTAGCTTGTTT[G/C]TTTTCTTAGCCTTGMGA TGACCAGGTAGAGAGACAGAGTGAGACCMCAGTTTTTCTGATTTCCCTGCTCCTCCTATTCCTTCCT

Wl-81 18f 1 1 4 AAAMTCAGACTCATTGTGACCAGTAGTCTTGAGGACTCMGCTGMTGA

TCTAGGTTFMTCMAGCMTTTGCANTTTGGATTTTGGA[A/G]TGACCACTCCCTTGCTMGGMGC TATGTACTTCATGCTGTGGAMCTGGCAMTACAGMTGTAGCTTGTTTGTTTTCTTAGCCTTGMGA TGACCAGGTAGAGAGACAGAGTGAGACCMCAGTTFTTCTGATTTCCCTGCTCCTCCTATTCCTTCCT

WI-8118Θ ! 40 A d- AAAAATCAGACTCATTGTGACCAGTAGTCTTGAGGACTCMGCTGMTGA

TCTAGGTTTMTCMAGCMTTTGCANTTTGGATTTTGGMTGACCACTCCCTTGCTMGGMGCTAT GTACTTCATGCTGTGGAMCTGGCMATACAGMTGTAGCTTGTTTGTTT[T/G]CTTAGCCTTGMGA TGACCAGGTAGAGAGACAGAGTGAGACCMCAGTTTTTCTGATTTCCCTGCTCCTCCTATTCCTTCCT

Wl-81 18d 1 1 8 ' T G — AAAMTCAGACTCATTGTGACCAGTAGTCTTGAGGACTCMGCTGMTGA

TTFTTAMTATGCCCGTTTAGAGCAGACACAGTCACMTMMGTTMAMGTTACMTGTGTCCAG TGTATATACCCAGGNMTCCATTCTTGGTACTTTTCMGAGCTGCTGTTATACTGAGTCTCTGAGMG TCCCCTTAGATMTAGCTGCCACTTTTCAGTATGGTTCAGMT[G/A]AGTATCTTAGTATTCTTTCTA

WI-8321 1 78 TTTTGCTATGGTTCTAGTTFATCMCCTACTTTATTAGCTGMCTGTTGGC

TTTTTMATATGCCCGTTTAGAGCAGACACAGTCACMTMMGTTMAMGTTACMTGTGTCCAG TGTATATACCCAGGNMTCCATTCTTGGTACTTTTCMGAGCTGCTGTTATACTGAGTCTCTGAGMG TCCCCTTAGATAATAGCTGCCACTTTTCAGTATGGTTCAGAAT[G/A]AGTATCTTAGTATTCTTTCTA

WI-8321 1 78 TTTFGCTATGGTTCTAGTTTATCMCCTACTTTATTAGCTGMCTGTTGGC

TATGTACTCACTTTCAGTTACCCCCGTGCCTCCAGMTCGCATGTTGCTCCACCTGGGGGCGGATATA MTTACCTCTAGATTGTCCAMGCCCAGTCTTTCCCTTCCCTGTGCAGCCTTAGA[A/C]ACTMGTAG CAGTACTGTTTGGTGTGTGTTTGTTTCTTCCCCAGCMTGCCTACTGCAGCTACTTAGTMCMCTAG

Wl-8332b 123 AGGTGGAGGGTNTCCGGGGMGCAGTTAGATGAGTTMGTGTGATGCACA

TATGTACTCACTTTCAGTTACCCCCGTGCCTCCAGMTCGCATGTTGCTCCACCTGGGGGCGGATATA MTTACCTCTAGATTGTCCAMGCCCAGTCTTTCCCTTCCCTGTGC[A/C]GCCTTAGAMCTMGTAG CAGTACTGTTTGGTGTGTGTTTGTTTCTTCCCCAGCMTGCCTACTGCAGCTACTTAGTMCMCTAG

Wl-8332 1 1 4 AGGTGGAGGGTNTCCGGGGMGCAGTTAGATGAGTFMGTGTGATGCACA ON SI

TGCGGGCTTMCAGGMGCATGACTGGGAGGCCTCAGGMGCTTATMTCATGGCAGMGGCGMGG GGMGCMGGACCTTCTTCACATGGCAGCAGGAGAMGAGMGMGGGAGMGTCTACACACTTTT AMCMCCAGATCTCATGAGANTTCCATCGGGAGACAGCACTAGGGGGATGGCACTAMCCATTAGA

Wl-8378b 31 1 MCTGCCCCCATGATCCMTCACCTNTCACCAGGCCCCTCCTCCMCACGTGGGG

TGCGGGCTTMCAGGMGCATGACTGGGAGGCCTCAGGMGCTTATMTCATGGCAGMGGCGMGG GGMGCMGGACCTTCTTCACATGGCAGCAGGAGAMGAGMGMGGGAGMGTCTACACACTTTT MACMCCAGATCTCATGAGANTFCCATCGGGAGACAGCACTAGGGGGATGGCACTAMCCATTAGA

WI-8378 308 MCTGCCCCCATGATCCMTCACCTNTCACCAGGCCCCTCCTCCMCACGTGGGG

^•AGCACATATTTAGCATTMGCCTCMACGATACAGCMTATGTTACATTCTCTTGTGAAMCAG TTGTTGTAGACTGTTMNNNNNNNNMATGTMCTCCGACTTGTGCCTMTAGGATTTGACCNTTAA GAGGNTTCTTTTGCTGTGGANGGGGTGGCTTTGCTTGMCTTCCATTCTGtT/G]GCCTTGTAGCTGGTG

WI-8426 1 84 G AGGCTGGGAGTATGGANGGNCCCGGGGCCCTTGGCNATNGNATFCAGTGAG

TTGAGCCTCCACAMTMTGCAACCMGTTTTACATTTTTMCAGCCCTTCTACATACACT[C/A]CA TCTTCTCTATCTTAGTTCCMGTTTTAGTTTTCMTCCCMπATACCMTTCCATTGTTATTTTMGA MAMCCTTCCCAGTTATTGTCAGAMCTATGATTTAGCTTACCCCCTCCACTACCCAGCAMCTAC

Wl-8450h 61 C A AGAGAGGATGGGAGTGTMTATGAGCAGTACAGAGTCTTMTGCMTTCAT

ON UI

ON 4i-

ON CΛ

ON ON

GGCCACTGTCCAMGTCTGTCACAGTCCTCCATATGGCAMGATGMGMMTTGGCMTCT TTA

GGGGTACCMGGNTCTGAGTTTGTACGGTCTTTATAMTGCAGAGCMGATGTGGCTTTCCTGCCCC[ C/AJATTTCACCTCMGGCATCTTCAGCMCCCCACATGGCTTCCCTCTGTGCGCATGAMTMCTTG

Wl-9676h 1 34 AGGCCAGGGTCTCTCAGCTTTAMGCCTTGGMTCCTATGCATTGTTTGTTT

GGCCACTGTCCAAAGTCTGTCACAGTCCTCCATATGGCAMGATGAAGAAAATTGGCMTCTTTTTA GGGGTACCMGGNTCTGAGTTTGTACGGTCTTTATAMTGCAGAGCAAGATGTGGCTTTCCTGCCCCC ATTTCACCTCMGGCATCTTCAGCMCCCCACATGGCTTCCCTCTGTGCGCATGAMTMCTTGAGG[

Wl-9676g 202 C/ηCAGGGTCTCTCAGCTTTAMGCCTTGGAATCCTATGCATTGTTTGTTT

GGCCACTGTCCAMGTCTGTCACAGTCCTCCATATGGCAMGATGMGMMTTGGCMTCTTTTTA GGGGTACCMGGNTCTGAGTTTGTACGGTCTTTATAMTGCAGAGCMGATGTGGCTTTCCTGCCCCC ATTTCACCTCMGGCATCTTCAGCAACCCCACATGGCTFCCCTCTGTGC[G/ηCATGAMTAACTTGA

Wl-9676f 1 84 GGCCAGGGTCTCTCAGCTTTAMGCCTTGGMTCCTATGCATTGTTTGTTT

GGCCACTGTCCAMGTCTGTCACAGTCCTCCATATGGCAMGATGAAGAAMTTGGCMTCTTTTTA GGGGTACCMGGNTCTGAGTFTGTACGGTCTTTATAMTGCAGAGCMGATGTGGCTTTCCTGCCCCC ATTTCACCTCMGGCATCTTCAGCAACCCCACATGGCTΓF/CJCCCTCTGTGCGCATGAMTMCTTGA

Wl-9676e 1 73 T GGCCAGGGTCTCTCAGCTTTAMGCCTTGGMTCCTATGCATTGTTTGTTT

GGCCACTGTCCAMGTCTGTCACAGTCCTCCATATGGCAMGATGMGMMTTGGCMTCTTTTTA ON -4 GGGGTACCMGGNTCTGAGTTTGTACGGTCTTTATAMTGCAGAGCMGATGTGGCTTTCCTGCCCC[

C/A]ATTTCACCTCMGGCATCTTCAGCMCCCCACATGGCTTCCCTCTGTGCGCATGAMTMCTTG

Wl-9676d 1 34 AGGCCAGGGTCTCTCAGCTTTAMGCCTTGGMTCCTATGCATTGTTTGTTT

GGCCACTGTCCAMGTCTGTCACAGTCCTCCATATGGCAMGATGMGAAMTTGGCMTCT TA

GGGGTACCMGGNTCTGAGTTTGTACGGTCTTTATAAATGCAGAGCA[A/G]GATGTGGCTTTCCTGCC CCCATTTCACCTCMGGCATCTTCAGCMCCCCACATGGCTTCCCTCTGTGCGCATGAMTMCTTGA

WI-9676C 1 1 4 GGCCAGGGTCTCTCAGCTTTAMGCCTTGGMTCCTATGCATTGTTTGTTT

GGCCACTGTCCAAAGTCTGTCACAGTCCTCCATATGGCAMGATGMGAAMTTGGCMTCTTTTTA GGGGTACCMGGNTCTGAGTTTGTA[CtηGGTCTTTATAMTGCAGAGCMGATGTGGCTTTCCTGCC CCCATTTCACCTCMGGCATCTTCAGCMCCCCACATGGCTTCCCTCTGTGCGCATGAMTMCTTGA

Wl-9676b 92 GGCCAGGGTCTCTCAGCTTTAMGCCTTGGMTCCTATGCATFGTTTGTTT

GGCCACTGTCCAMGTCTGTCACAGTCCTCCATATGGCAMGATGMGMMTTGGCMTCTTTTT, GGGGTACCMGGNTCTG[AC]GTTTGTACGGTCTTTATAMTGCAGAGCMGATGTGGCTTTCCTGCC CCCATTFCACCTCMGGCATCTTCAGCMCCCCACATGGCTTCCCTCTGTGCGCATGAMTMCTTGA

|WI-9676a 84 A C GGCCAGGGTCTCTCAGCTTTAMGCCTTGGMTCCTATGCATTGTTTGTTT

TGGACCAMCACAGACAGATGTATTCCTGGTGCCTGTGTA[C/AJATTACMCTCATTGATCACATGC AGCMCATCMCATCTCMGGAGTCCATTTGTTCAAAACACAGTAMTGACTCCACATTTTCCCTTT GAGTCMCAAMGACTCTGCTTGTCACCTTGCCTGGAGCGGGGTGGTTTTTCACTATGTGAGTATCTA

Wl-9738b 40 TCTTTTTATTTCTGTCCCTTATGTTGGTGGGCACATGTCTGTATTGCTGTCC

TGGACCAMCACAGACAGATGTATTCCTGGTGCCTGTGTA[C/AJATTACMCTCATTGATCACATGC AGCMCATCMCATCTCMGGAGTCCATFTGTTCAAMCACAGTAAATGACTCCACATTTTCCCTTT GAGTCMCAAMGACTCTGCTTGTCACCTTGCCTGGAGCGGGGTGGTTTTTCACTATGTGAGTATCTA

WI-9738 40 TCTTTTTATTTCTGTCCCTTATGTTGGTGGGCACATGTCTGTATTGCTGTCC

ACTGAMTGTAMTGGCCMGGCACCCAGGACCTTAAAMTCATMGMGTTMTCTGTGGGMM GAGTMCTACAAMGCATCTAMCMGAGCAGGATGTGATGTAATGTGTCCCCTTATCACTTTAGTC AGTAAAGATMGMAGCCCTGGTGAGTATCCACTTCCACAMCACACAGMTATACACTTTTGGMG

WI-9756 47 ATΓTCCACTTMCCACTTGATTCTTCAC I I I I I I ATGATTTAAAACTCTCCGTGG

GATGGTCCCTTMGGATTTGCATTGGTTMTGGGCAGACTGGTGCAAMGAGGCTGMTTGMTMT TAGGMACTGGGAGMTTCAATTCAMGMGMTTCTTGTTCGCMGGTCMTTTTTATACTATTTA A[A/G]TAAAATAACTCTGGTAGGTTCTATAGCAMTGCTAAGTAAAGTAACCGCTGGTTTCTAAATT

WI-9758 1 35 A G ATTACG

ATTTAAATCCAGGCAGCGGGGAAMTGGATACTTTCATATGTCTCTGTACCCMCTATAMCTTTTG ON CO GTTCTCATGCACCATTTTCATTTTGCCTTCTCACTCCMGTACCACTGATTTTACCMTT[G/A]CTCTC ATMTTGACTTTGCTACTGGMGAMCTCTTAGMTGTFGGMTTTCTCTATTACACACTTTGCCTCA

WI-9778 1 27 MGMTGTGTCAGTCAGGACTAMGGCMTAGTCTCAGGGCAGACAGCC

TCTCCCCTTTGCCTCCTCATGCCCACTCCCTCAGCCTGCACAGAGCGTTTCTCCAGTGTAGTCTCTGGT CCATCTGCATCAAMTCACCTGCAGGACTTGCTGACMTGCAGTTTC[C/A]TGGATCCCACCCAGGA CTCAAAAAMCTAGGMTTGGGAGMGAGGGACCTGGMTCGGTGTTGCTAGCMGCCCCCAGGTGG

WI-9832 1 1 6 A TTTGTMGTGGACTAMGTTTGAGGACCAGACATGGMGGTTGGCTTTGGC

TGGAMMTAGC^" TTTATCAATCTCTGATATGCTACATATGTCATGGAGAMTGCAGMTGGCATGA TATGAAATTCCA^" TTTTGAATGAATAAAATATAC[A/G]TGTGTATGTATATATACTTATFAACACTT

AGGATTATATACACACMTMAACGTCTGTMGGATAMCTMGGTTCTATCAGTGGGAMTGAGA

WI-9841 1 01 G- TTGAAMGAGGGGGATGTGTTACTTGATATGCTGTTG

GMCTMCACCTTTCTTGCATGGA I I I I I CTTGATTATTGGCAGTTAACMTMMTGTTATTAGATC ACTGGTGCTTCTGTGTGGGGTTGAG I I I I I l ATGATATCTCCTGTTAGACCCATMGGGAGGCTGTGA GTTGTTTTCTACATCCTTGGACTATATAAGATCCTCTTTTMMTTATATTTTATATMGCACATGAA

W1-9880C 222 G A AATGGAATGAAATAATGAfG/AITTGACATAGGAATTACCTACATATTTTG

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4i.

-4 CΛ

AGTATACAMCATTTMGCTGTGGTCMGGCTACAGATGTGCTGACMGGCACTTCATGTAMGTGT CAGMGGAGCTACAAMCCTACCCTCA[A/GJTGAGCATGGTACTTGGCCTTTGGAGGMCMTCGGC TGCATTGMGATCCAGCTGCCTAπGATTTMGCTTTCCTGTTGMTGACAMGTATGTGGTTTTGTA

DWU-252 94 AT

GMCATTCCTCTGCAGCACTTCACTACCAMTGAGCATTAGCTACTTTTCAGMTTGMGGAGAAM TGCATTATGTGGACTGAA[C/ηCGACTTTTCTAAAGCTCTGMCAAAAGCTTTTCTTFCCTTTTGCM CMGACAMGCMAGCCACATTTTGCATTAGACAGATGACGGCTGCTCGMGMCMTGTCAGAM

DWU-330 85 CTCGATGMTGTGTTGATTTGAGAMTTTTACTGACAGAAATGCMTCTCCCT

GAAAATGTTAATTGGGCAGGTGAAMGGGTACAGATGTGCTGTAGCAGACCTTTGGTTTTAAMGAG

MGCATCATTTCCCCMCAGGGCMCTGTAGMGGCCAGCTGMGAGTAMGGAAMGGTCTGAGG

ACTGAGCCTGTGGCTGGCTGGAAAMGGTGMTGTTGAGGGCCCTTCACTTCCATCACMGAMGTC

DWU-370 231 i ATTAGACGGTACCMTTCAGTGTCTGTTCCT[A/G]GCATCTATTTCCTCTGTGC

CTCTTAACTTCAGTTCCCTCATCTATAAGMTAAGGGATTCAGTTGTGATCACATAGCTCAGGTAATC

DWU- CAGGACCAGAMCCCAGGAGC[A GJTGGGACCTGATCCACAGCTAGAGGATGGGGGACTCTGTAGCT 1537b 89 ACAGCATTTTCCTGMCACACMGMATCCAGTMGCAGCACACACTGGCTGA

CTCTTMCTTCAGTTCCCTCATCTATMGMTMGGGATTCAGTTGTGATCA[C/T]ATAGCTCAGGTA! -4

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ACCATCTTATACTATGGCAGGTMGTCCATACAGMGAGCCCTCTCTCCCTGGGATTTGAGTGGGGTC CCCAGCTCCACCCAGAGGCCCCTGGGGMTTCCAGGGTCACTGTTCCTTCCTGTCTCCCTGTGGGMT

ESTD- CMGCCAGCTCCAGGCCAGMGTGGGACTGTGAGGACATGGAGGCCTCGGCACTGAGCTG[C/G]AGA ADAb 1 96 CCCGCAGACCMCTCCTGAGCTTTCTGGGCCTCTGAGTCTTGTCCTC

ACCATCTTATACTATGGCAGGTMGTCCATACAGMGAGCCCTCTCTCCCTGGGATTTGAGTGGGGTC CCCAGCTCCACCCAGAGGCCCCTGGGGAATTCCAGGGTCACTGTTCCTTCCTGTCTCCCTGTGGGMT

ESTD- CMGCCAGCTCCAGGCCAGMGTGGGACTGTGAGGACATGGAGGCCTC[G/AJGCACTGAGCTGCAGA ADAa 1 84 CCCGCAGACCMCTCCTGAGCTTTCTGGGCCTCTGAGTCTTGTCCTC

TCTCCTGTCATTCCTACTCCATTAGTTCMGGTCAGTGMGMCTGGGGCMTTMCCMGTMTTCA

ESTD- TGGACTGCCCMCTGCGMACMGMGGGCGCAGTGGAGCAGGAGTATTATGCTACGCGGTTACCTT ANT1 1 60 Tl TTTTTATGGAGGACCGMCTGAGGCrr/qGAGCTCAGATGATCCTGT

TGCCTGGGGTGGCMGGCTGCAMCMGGAGGCMCCCAGGAGGCTTTTATGMGCGGGCCATGGTA

EST10398I AGATGCTGCCACCTCTTATCTACTTGATGATGTTCACATTTGGGGCTTGACTTTCCMCACGGAGMG 2b 1 68; A' G - CATTGTTTTCTTCGGGCCMGMGGTATCTACCrA/GIATAGTGTCTATTAGGCATTTG

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AAAGCATGAC CGCTTATGTTA AATAAAATGA ATAGTMTTCC CMGTGAATATTGATACATGGCTGACMAGCATGACMTMMTGMCAC[A/G]TACGGGMTTAC

WI-17904 50 G ACAC 03 TATTAACATMGCGATAACATCAAAACATCTGGTAMATGCAGTTAAAACMCAACACAMTGA

TGCCAMTAC MCTACTAGCG G I I I M I CTTTGAGTGACACMGCTTGTTCA I I I I I GAGAAMTGTGTGCCMATACTCMGTGTGM

EST34149 TCMGTGTGA AGMCAACTA T[A G]GATTTTATTAGTTGTTCTCGCTAGTAGT ΓGGTATTCTATGMAMMGCAGCTAGTTCAGC 5 69 AT ATAAAATC TT ACAAATCACACAAGT

TGGGAAMCATMGTTMCTCMGMTATATFCCAGTCTTTATGTTACTMMCATTGTMTAGTGT

EST34343 TTTTATCAATGATGCCGAGGTCACTGCT[C/A]TACAMGATTAMGMACTTACCATCAAACACTTC 8 95 CAGTGCATCM

GGACCATATG CAGAMTTATG GGTACACAATTTTMTGGMGGMCCACAGGTATGTTGAMGMCATCAGTACAGCTGGAGACAGG ATATATAACT TGATAATAACT GAGGGACCATATGATATATMCTCCTAMAGC[C ]GGMGGAGTTATTATCACATMTTTCTGGGC

WI-17982 98 CCTAAMGC CCTTCC GCTACAGMG I I I I I CATCA

CTCAGTMCTCCGGTGTATMTCTGCCATTTATTGATTTATTTATGATAAMCMCCTCTCATTGTGA AAMCAGCTMGGGTGACATCTCCAGACCCMCCACTGTCCCTGTMTGT[A/C]CTGCTGAGAGTCC

WI-17993 1 1 8 ACATTTTGGAAATCCAAT

CCCATCCAGAMCCCCAGTGTGATGGTGGMGCAGCATGAAMCMCATCTCCCCAGGCCTCGCAGT

GTAGAGGCGA AGGCACATGGG AGAGGCGAAGGGMCAG[A/GJGCTGCCCATGTGCCTGTCTCTAMGACGCCACCCTCAGGTTGATGT

WI-17996 84 G AGGGMCAG CAGC CACCTGTGGGAGACCGGGT

ATTCTTTATAAAAACACCATGTCCCTAAAATGT[C/GJATTCMCATATATGCACACCTTCGATGTAT

WI-17136 33 AGGACACTGATCAAMMGACAGAGAMTGTGTCCCT

GCCACTGAAAAMGGTGCTCTTCC[A/C]GTTTCTMCTCCCTGGACTCCCTCATTGGMCTGMGCTC ACAGATGTTTCAGCTGGACTAGT1TAGACTTTGCTGTATTTTAMAGGCAGTGTTGATGCTCCAGGAT

WI-18041 24 TCAAATACTTAATCA

EST35164 CACAGCCCTGC CCCTCTGGATT TTGMCCMGGCCCTMCAGATGACTCAGCAGGGCCTTCMGCACAGCCCTGCCCCC[AG]TCTTGA 8a 57 G CCCC CTGMTCTCM GATTCAGMTCCAGAGGGTGCTCAGTCCTTGGTTTAGGTGCTTCTGTGACATTTCCTCTTG

AGCGMTGMMTGCTACATAGGCTCCCTGAGTTCTTTCATGTACGMTCTTGGTTACACATCTTAGl

Wl- AGJACAGCAGAGCTGCCTGAGGGAGGGTTGTGTTTMTGTCGTATGCATGCTCAGCACAGTGCTGGC 18052b 67 ATGGCCCATCCATGCTTT

CCTGAGTTCTT AGCGMTGMMTGCTACATAGGCTCCCTGAGTTCTTTCATGTACGMTC|T/C]TGGTTACACATCTT

Wl- TCATGTACGA CTCAGGCAGCT AGMCAGCAGAGCTGCCTGAGGGAGGGTTGTGTTTMTGTCGTATGCATGCTCAGCACAGTGCTGGC 18052a 50 ATC CTGCTGT ATGGCCCATCCATGCTTT

GGGAGTGGGG CGTCACCCTGC CTGTTGTGCTGAGMCAGMGGGGTCMGGGAGTGGGGGAGTAAAA[G/A]TGGMGCAGGGTGACG

WI-18054 46 GAGTAAAA TTCCA CATGCAGGAGTCCAGACAAMGACGGGTGATTTTGCTCAGGTTGGTAGCMCAGAGGTMTG

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EST39236 ATAMCTTCCTj CTGAGTCATAC TAATGTATGACTCAGTACCTATMTGAGACTGGAMTATATTACCTGGCAMTGMTGAGGTGTCTC Ob 57 GTCT ATTAAACA

GCACMTTAA CAMCAGACCTTTGGTTTGAGCTCACCTGGTGACAGGAGACTCCTACCTGAMCAGGGATGCC[G/η

EST39294

CCTGAMCAG ACATAGTACCG TTCTCGGTACTATGTTTMTTGTGCTGAGCCAGCMCCCTCGAGTTACCCGGCCTTTTACCCCACGCC 4 63 GGATGCC AGAA AGCTCTGCTTGTCTGCAT

AGMAACATTCTGTCTGATCAGAGGMGATGTATGTAGAAMTCAGMTCTGACTGMTTCCTMA

EST39366 ATCTAT[T/C]ACACTGAGAGGAAAATGGAAAAGAAMTGTTTGCATAMGCTTTTCCCTGACTCTCA 2 GAGGGGTTCAGA

TGATTTGAGAC AAAMGCTGTAGCTGGCMGTCAMGTTTATTTTATGTGTGTAMTTCCCAGTTGAGCATTFTTTCAT

EST39371 CATTTGGATTA ATTTCACATTT TTGGATTAGCGTGAGAGG[A/G]AAAAATGTGAMTGTCTCMATCAAATGCTTCCTTCTMAGATTA 9 86 GCGTGAGAGG TT GACATTGCCCMCCCTGC _

ACMGTGACATATCCMCCMCC[A/G]TCCATCCCCACCTGTGCCCTATTCTTTCCTTGTGTTTCTTT AGAGCCTTTTCAGCTATTTCCTGTGMGCAMCTGCACGMGGCCTCCCCCGTACTCCTCCCCTGGM

WI-17177 23 G _

AGGTTCCTGGTTGCTCCCCACMTTTTGATTIC/TJGGTGGCTTCATAAGGGACCCAGGATFCTGCATT S

EST39428 GCTCCCCACA GGTCCCTTATG TTCTGGGTGGGGCCTAGGTMTTCTGTTGCCTTTGGTCCACAGAGCACMTTAMGMGATCAGGTCT CΛ 8 31 T ATTTTGATT AAGCCACC GGCTGTTGC _

GGCAGAGGM

EST39430 TMCTGATGTT CAGGGGTCGGG MTTTAGCAGAMCMTGMGTTGGCAGAGGMTAACTGATGTTC[A/C]CAATACCCCGACCCCTGA 2 45 C GTATTG CCCAGTACCTTTCCCTCAGGCCCAGGCTCCGGTGGAGGATGTCCTGGG

CTACTGACAT AAAGCCCTGTAMCTGMGCTAGACMCGTCMCTTTGGMGMAATMCAGGMCCTATTTATAT

EST39446 AGGGACTTCA TCCTGGAAMC ACGTAMTCACTTTCATACCTGCCTACTGACATAGGGACTTCAGAGTMTA[CAF]GGTTTATGTCAGT 7b 117. GAGTM TGACATAMCC TTTCCAGGATTGTTCTCCC

EST39465 MTGCAGGAG CMTCTCGGCC ATGGTGTCATTAGAGGGCCACAGGGGATGGGGGAGTAAMAATMCATAMCGMCTGMCAGAM 2 80 GGTGGC CCTCT TGCAGGAGGGTGGC[A/G]AGAGGGGCCGAGATFGGGTGTTCAGGGCAGAGAGGTGGMGACCAG

AMGATTCCT

EST39501 GTAGACATCT CACTTGCMTT TGCTTACMCCCATMCCATAGGCCATGTGTTCAGACATTCTTGACCMGCCTMAGATTCCTGTAG 0 81 MCATTAG CTGMGGCT ACATCTMCATTAG A/GJTAGCCTTCAGMTTGCMGTGCMGTTCAAGTCMACCMTTC

CACAAMTGGGACTGCTGMGAGTGGACAGTTGGACCTTACTTTGGTGACCCCATACATTTGTGGTCAI

Wl- CATGCTTTAGCCATAqA/C]CATGGTMCATTGACTATGGAGTCTTGTGAMGTGTMTGTGCGATG 1 8387b 84 A C - GCTATGTAGACATAAAGA

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CAGGCAGGACTTCAGTGTCAGTATCCCTGCCTTCAGTCTTCTTTAGAMTCACATCTGTGTTCMTCC ATTGTTTAGAGGGAGTGTA M i l l CCTGTTCCA[C/ηGMGAGGACTTTTTGTTCACMTTGGATCAC

D63807 1 01 T ^■ MTGCAGAGGAGTCTGTTCCTCCCCCGTCGGCTTCTCGGTGCTGGGAGGGTGACCTGTCCCAGATGAC

TGGGMCATGCGTGTGACCTC[T/C]ACAGCTACCTCTTCTATGGACTGGTTATTGCCAMCAGCCACA CTGTGGGACTCTTCTTAACTTAAATTTTAATTTATTTATACTATTTAG I I I I I ATAATTTATTTTTGAT TTCACAGTGTGTTTGTGATTGTTTGCTCTGAGAGTTCCCCCTGTCCCCTCCACCTTCCCTCACAGTGTG

D90145 21 C - TCTGGTG

EST14035 ATTATCACTCTCMAAATTTTGGTGTGTGTGTTTMGTACTTTCTTATTTATGAGCCCC[T/C]GAGGA 1 a 59 c - CCAGACATGTTATTATCMGCCCCTTATATACCATCTMT

EST16668 GCATTTTAAAATTCACATTGMTCATTATTTACTATTTATGATGTTTACATAACMTTCAGTATCATT 5 71 T ^■ ATG[C/T]TGTAGATTTCAGATGTAGGTCGTCAATACTGAGCACTTATCT

EST16904 ACAGACTATCGCCAACTTATMTGCTTAAACTTTATGATCMTAGTAATAMTTACA[C/T]GAGATA 7 5_7 T TTCACACTTTATTATAAAATAGGGTTTGTGTMGATGA I I I I I CCCMCTGTAGGTTMCAT

EST21863 TTTTTMGTACCAGAGGCACTGCTGGMCAGGATGAAMCTGATACACCIA/G]GTTACTACTTACTC 9 49 G - TTCACTCTTCAMCTGATTCCCCTAMGACTTCTACTTAGCAM

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EST21885 GGCTGTMGTAGAATCAMGGTTMGMCATTTTATGCACTTATTCCACAAACATTTACTGAGCATA SI

UI 6 80 A ^■ CTAGGTGCTGGGA[G/A]TGTGACAGTGAGCAAAMACACM

EST22623 ATTTTAGTGCAAATGACAMGCCCAA[A/G]AGMCAGAGGATCAMTAAGATTGAAATGTATTACC 8a 26 G_ TTCTCATMGTATACGAAGTTTAACACMGTATGGGAGT

EST22644 AAAATGATTGMTTCAGCAAGTACATTTATGATCTATCTACATTGTTAAAACAGCACTAAAAATAA 2 98 G MA M I L L AAAATGATTATCCATTATTTACAG A/GJAAATGTGGAAAAGATGGCTTTTAAACCC

EST23587 | CCTCATTTATTTAAAAAGACGGACATAAAAA[T/A]TATACAACAAAMACCCAAGTCACATTTCAG 1 31 A GAGGTAAMACTAAAMGTCTGATATGAMATATGGTGG

MAGATCTGGCATTATTCACATCATTCTMATATTTTGTAATTAC I I I I I CCATGAGTATTTTTTTCA

EST24246 TGTCCMGCATTTTMCTATCATTTTAGCGTAMTACCΓF/C]GMTAACCCATAGTTACAGAATTGG

7 1 06 GTCTGTGTMCCTCMTT

TAGTTTMTTTTCTGMCCTTTGGCTTATAM I I I I I CTCAACTT[A/G]CATTTAAAMTGTATCAAT 45 GCACCTTCTTCAGTAGTACCACATGAAMTATAMCCTCGTTC

EST24435 CTTGMCTTCTGGTCTCMGTGGTACGTCCGTCTCMCCTCCCAAMTGATGCGATTACAGGCATMG

(3 73 CAGCCLGYALTGCCTGACCCACATTTFCTTTATCCGATCTGTTGATGGACATTCAGGTTGTTTC

EST25089 TATTGTTGCATTATCAAAATGGTTAΓF/C]AGTTTTCMTTAAAACTGTAATTGATTTCTATGTATAAA 6 25 ACAGCTFTGMGTTGTMATGTAGTTTCCMTCGTTAGTTMTGCTACATT

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AGTTGCCAGCTCCCATGTACCAGCAGCTGGMTCTGMGGCGTGAGTCTTCATCTTAGGGCATCGCTC CTCCTCAC[G/A]CCACAMTCTGGTGCCTCTCTCTTGCTTACAMTGTCTAGGTCCCCACTGCCTGCT GGAMGMMCACACTCCTTTGCTTAGCCCACAGTTCTCCATTTCACTTGACCCCTGCCCACCTCTCC

U31416C 76 MCCTMCTGGCTTACTTCCT

AGTTGCCAGCTCCCATGTACCAGCAGCTGGMTCTGMGGCGTGAGTCTTCATCTTAGGGCATCGCTC [C/ηTCCTCACGCCACAMTCTGGTGCCTCTCTCTTGCTTACAMTGTCTAGGTCCCCACTGCCTGCTG GAMGMMCACACTCCTTTGCTTAGCCCACAGTTCTCCATTTCACTTGACCCCTGCCCACCTCTCCA

1131416b 68 ACCTMCTGGCTTACTTCCT

ACGGGTCACACAGAGAMCCTGAGTCTAGCCATGAGGGGCTTATGCTCCCMCTCACATTGTTCCTCC AGACCGCAGG[CTJTCCCCCAGCCTCAGGTTGCTGGAGCTGTCACATGACTGCATCCTGCCTGCCAGG GCTGCAMGCMGGTCTTGCTTCTATCTGGGGGACGCTGCTCGAGAGAGGCCGAGAGGCCGCAGMC

U37519a 78 ATGCCAGGTGTCC

GACCACGCTGAMCCCACCCACCCGCTGTGCTGACCATGGGCCCTGAGCGTCCT[A/G]CCCCGMTTC ACGAGGCTGAGGCATCCGGGAGCTGGCGTMTGCCTGGCCGCAGTGTGTGTGTATCCCATACCCCACT

U37690 54 A^! G CTGGMGGMCCATCCAGTAMGGTCTTT

TGAAACCGTTTCMCATGGAAATGATCTGTATTGACTM[T/C]ACACCAGTCCACACTTCTATGACT ) UI TCTGCCATTTCAMGACTCATTTCTCCTATMCCACCGCATGAGTTGMTCAAMTTTTCAGATCTTT SI TCAGGAGTGTMGGMACATCATGTTTACCTGTGCAGGCACTAGTCCTTTACAGATGACCATGCTGAT

V00540 39 A

TCMGMGGTGACTGCCCTTGTATGATGGGATGGGMGATGAATGACTGGTTTTTACTGGGGTGTM AACCACTCTGAGCCTCTCTGAGACCATGTGGTTTTAAM[A/ ATCCATMGGGMGGTACCCACAC CAGTATCTGAGTTCCAGTAGCTMGACCCTAGMTTTGGATTCATCTCTG I I I I I I CATGTCTCTCCTT

X15943 1 06 GTMCCCTGAGATCATCAG _

AGGMGATCCCACCGACCCTTCCTGGCCTMTCCTTTAGATFAGGTCACATTACATTMCATTTAGGA ACCCAGACCGAMAGTTGCTGAMGGGMGGAGACACATTCACAMGAAMGTTGCGMMTTGCG MATCTGTTGTGCA[C/ηGCTCAMTGMAACGCCTTTCGGCTTTGGGCTTTTA I I I I I I I GGMCTG

X5201 1 b 1 48 T CGAGTGGCTTAGGTCTAGCCT

AGGMGATCCCACCGACCCTTCCTGGCCTMTCCTTTAGATTAGGTCACATTACATTMCATTTAGGA ACCCAGACCGAMAGTTGCTGMAGGGMGGAGACACATTCACAMGAM[A/C]GTTGCGAMATT

I GCGAMTCTGTTGTGCACGCTCAMTGMMCGCCTTTCGGCTTTGGGCTTTFA I I I I I I I GGMCTG X5201 1 a ' 1 1 8 C - CGAGTGGCTTAGGTCTAGCCT

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CATCCCMGGCACTGGTGGTGACTCTGCTTCCTG[C/T]ACTGACCCAGAGCCTCTGCCTGTGCACTGC MGCTGTGTCTACTCAGGCCCCMGGGGACTCTCTGTTTCCATTCTCCCCCCACAGACCTGTCMGAG

X87344 34 T MGCATGACAMCMMTCATTTACCGACTTTAGTGCTTTTTT

GGTGGGCTGGTATCTCAGAMGTGCCTGACACACTMCCMGCTGAGTTTCCTATGGGMCMTTGA AGTAMCTTTTTGTTCTGGTCCTTTTTGGTCGAGGAGTMCMTACAMTGGATTTTGGGAGTGACTC MGMGTGAAGMTGCACMGMTGGATCACMGATGGMTTTA[GtηCAMCCCTAGCCTTGCTT

X87838 1 79 G GTJAAAATT

GTTCTGCTGCCTCTACACAGGGGCCCTGTACAGTGMTGGTGCCATTTTCGMGGAGCAGCAGTGTGA CCTCCTGTGACCC[A G]TGMTGTGCCTCCMGCGGCCCTGTGTGTTTGACATGTGMGCTATTTGAT ATGCACCAGGTCTCMGGTTCTCATTTCTCAGGTGACGTGATTCTMGGCAGGATTTGAGAGTTCACA

Z14138 81 GMGGAT

TMTCCTCACCATTCCTCAGGTATMGTTCTATAMCAGGCTTGGMTCTGGGTMTTMAMCAGA AMTTATAGTCMTATACCATGACATGMGMTGAATCCATTCTTTGGAGATGGAGTATACATGACT GCMCTGTATTTCATACGTTCTTTTCAMGTGGGATAGCTATTGCAGCTTAMGAGC[A/C]CAGGTTC

Z18859 1 91 CAGTACTGGTTTTCCM

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Z23091 1 59 G CTGAGCTTGACGTTTG

GTTGGCATTGTTAGTAAMCTTCATAGGTGMGAGGAGGATCAGTGAGATTMGTTATTTTATCAM GTGTGGTTTTCTGCMGGGCAGGTTTGAMCCTGACCCTAGTTGTGCTCCAGGACCTAIA/G]GCGTGC TCACTCTACCTTGTCTTTGTGTTGAMGGAGTGGTTTCCCATGACTGTTTMGTGACMGTGCCATGG

1 1595b 1 25 ATATCTACACCGTCACCAGACTAGATTGTCTCMTGTCCTTGGCTTGCGAC

GTTGGCATTGTTAGTAMACTTCATAGGTGMGAGGAGGATCAGTGAGATTMGTTATTTTATCAM GTGTGGTTTTCTGCMGGGCAGGTTTGAMCCTGACCCTAGTTGTGCTCCAGGACCTA[A/G]GCGTGC TCACTCTACCTTGTCTTTGTGTTGAMGGAGTGGTTTCCCATGACTGTTTMGTGACMGTGCCATGG

1 1 595 1 25 ATATCTACACCGTCACCAGACTAGATTGTCTCMTGTCCTTGGCTTGCGAC

TATATCACATTAGTATGTCACTGCCATGGTMGGACTTTGATCACTAGGAAATMGMCACTTTGM TGGTCTTGTCCTTTCMTMMAGAGTGACATGATTGMCATGTGTTTTAGATMAGGGCACTT[GtF ]GCAGGAGTGTTTAGGATGMGAGAGMGAGATTMGGMGATCAGGMGMMGTAGCMTGGGA

1241 1 31 G T ATGAMATAGGAGGCCCTGAGATCCACTGGATMTCTMAAMCCMGAGAMG

GTGCGATCACCACTACAGTCTMTTTCAGATGFTTTCATTACCCCTAMAGAMTCTTGTACCCATTA GCMTTATTCCTCATTCCTGCCCTCACCCCCAGGCCCTACTCTTTATCGCTATAGATTTGCC[C/ηACT TGACATATCATACACATGGAGCCATACATATGTGTGCCCTTCATGATTGGCTTCTTTCACTGAGMTA

1 282 1 30 ATGTTTTCAAGGT

AGTATCACACATACTTAATATATTAGATATACACMTMTMAATCACTCCCTACCTTGAAMCTTT A[C/ηAGAAGCATTTTTAATTTTACAACACAMGCTCAAACGMCCTACAATMGTCTAGTAGTCTG TTTACGTGCCAAGGGATAAGGCTGAACMTAMTTMCCCTTTAAAAATGTCTATGMCAAGTACAA

681 0 68 T TTTTC I I I I I GAGTTCTGCAGAGCAATGACCACTMGMATA I I I I I AMGGC

CCMGTACATTGGGTGMCGATGAGCTAGCTGTTCTAGTATTTGC I I I I I GTMTCCAGTTMGACCA TCAGCATATACAACATCATCACTMCTCMCMTGTAGCTGCAGGGTMC[A/C]TGTGGATACCCTG TGTGCTCTACTGGCCTCCAMGGCATTCAGGGGATCATCAMGATGTTGGACACCTTGTGTTCAMTC

681 7 1 1 8 πGGTTCAGGTGCGGCCTGTGCAGΛTCGGCTTTTTGGTTTGGTTGTCTTAG

CCATTTTA I I I I I CTCTAAATTTTAAAATAGMGACTTTMTGGAAAACATTFAGTACCATCATGTCA CCCTGMTGCCAGCMTACCTCGACTTTTACACACGCAGGMGCCTAGTAAMGCCCCGTCAGTAGT ACACATTTCTCTATGGTCCTTCMCAGTTTTGCATATACAAMTTTTCTGCTATTΓTGCTTTAGCAAA

6819b 21 2 CAGCMTMCTTTTGTGTTTCCTATATGACACCTAATATCCA SI u>

CCATTTTA 1 1 ΓI FCTCTAAATTTTAAAATAGAAGACTTTAATGGAAAACATTTAGTACCATCATGTCA CCCTGMTGCCAGCMTACCTCGACTTTTACACACGCAGGMGCCTAGTAMAGCCCCGTCAGTAGT

ACACATTFCTCTATGGTCCTTCAACAGTTTT[G ηCATATACAAMTTTTCTGCTATTTTGCTTTAGC

6819a 1 66 UT AMCAGCMTMCTTTTGTGTTTCCTATATGACACCTMTATCCA

CTGGTATGTCATAAGCMTCCATAATTGTTATAGCTATT[A/G1TTATACTATGGCACCATTTGGGACA

CAGATTATATATGTCAGACACCACGMTGTCCTTTMGATATGCAGCMGCACAMTCTGTCATGGT

681 xx 39 TTAACAAMGAAATGMCGTCTAGG

AGGATTCCCTCTTFTFCTATTGATTGGMTAGTTTCAGMGGMTGGTACCAGTTCCTCCTTGTACCT CTGGTAGMTTCGGCTGTGMTCCATCTGGTCCTGGACTCTTTTTGGTTGGTAMCTATTGATTATTGC CACMTTTCAGA[GtηCCTGTTATTGGTCTATTCAGAGATTCMCTTCTTCCTGGTTTAGTCTTGGGA

6972b 1 49 GAGTGTATGTGTCGAGGMT

AGGATTCCCTCTTTTTCTATTGATTGGMTAGTTTCAGMGGMTGGTACCAGTTCCTCCTTGTACCT CTGGTAGMTTCGGCTGTGMTCCATCTGGTCCTGGACTCTTTTTGGTTGGTM[A/G]CTATTGATTA TTGCCACMTTTCAGAGCCTGTTATTGGTCTATTCAGAGATTCMCTTCTTCCTGGTTTAGTCTTGGGAl

6972a 1 22 ' © ^■ GAGTGTATGTGTCGAGGMT

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EQUIVALENTS

While this invention has been particularly shown and described with references to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Those skilled in the art will recognize or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described specifically herein. Such equivalents are intended to be encompassed in the scope of the claims.

Claims

CLAIMSWE CLAIM:

1. A nucleic acid segment shown in column 7 of the Table, or a portion thereof which includes a polymorphic site, or the complement of the segment or portion thereof.

2. The nucleic acid segment of claim 1 that is DNA.

3. The nucleic acid segment of claim 1 that is RNA.

4. The segment of claim 1 that is less ^'than 100 bases.

5. The segment of claim 1 that is less than 50 bases.

6. The segment of claim 1 that is less than 20 bases.

7. The segment of claim 1, wherein the polymorphic site is biallelic .

8. The segment of claim 1, wherein the polymorphic form occupying the polymorphic site is the reference base for the fragment listed in the Table, column 3.

9. The segment of claim 1, wherein the polymorphic form occupying the polymorphic site is an alternative form for the fragment listed in the Table, column 4.

10. An allele-specific oligonucleotide that hybridizes to a segment of a fragment shown in the Table, column 7 or its complement.

11. The allele-specific oligonucleotide of claim 10 that is a probe .

12. The allele-specific oligonucleotide of claim 10, wherein a central position of the probe aligns with the polymorphic site of the fragment .

13. The allele-specific oligonucleotide of claim 10 that is a primer.

14. The allele-specific oligonucleotide of claim 13, wherein the 3' end of the primer aligns with the polymorphic site of the fragment .

15. The allele-specific oligonucleotide of Claim 10, which is selected from the group consisting of the nucleotide sequences of the Table, column 5.

16. The allele-specific oligonucleotide of Claim 10, which is selected from the group consisting of the nucleotide sequences of the Table, column 6.

17. An isolated nucleic acid comprising a sequence of the Table, column 7 or the complement thereof, wherein the polymorphic site within the sequence or complement is occupied by a base other than the reference base shown in the Table, column 3.

18. A method of analyzing a nucleic acid, comprising obtaining the nucleic acid from an individual; and determining a base occupying any one of the polymorphic sites shown in the Table.

19. The method of claim 18, wherein the determining comprises determining a set of bases occupying a set of the polymorphic sites shown in the Table.

0. The method of claim 18, wherein the nucleic acid is obtained from a plurality of individuals, and a base occupying one of the polymorphic positions is determined in each of the individuals, and the method further comprising testing each individual for the presence of a disease phenotype, and correlating the presence of the disease phenotype with the base .