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WO1994026934A3 - Human papillomavirus detection assay - Google Patents

Human papillomavirus detection assay Download PDF

Info

Publication number
WO1994026934A3
WO1994026934A3 PCT/US1994/005085 US9405085W WO9426934A3 WO 1994026934 A3 WO1994026934 A3 WO 1994026934A3 US 9405085 W US9405085 W US 9405085W WO 9426934 A3 WO9426934 A3 WO 9426934A3
Authority
WO
WIPO (PCT)
Prior art keywords
assay
complexed
amplified
specific
solid phase
Prior art date
Application number
PCT/US1994/005085
Other languages
French (fr)
Other versions
WO1994026934A2 (en
Inventor
Janice T Brown
Original Assignee
Baxter Diagnostics Inc
Janice T Brown
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter Diagnostics Inc, Janice T Brown filed Critical Baxter Diagnostics Inc
Priority to EP94917931A priority Critical patent/EP0655091A1/en
Priority to CA002139623A priority patent/CA2139623A1/en
Priority to AU69456/94A priority patent/AU6945694A/en
Priority to JP6525595A priority patent/JPH07508891A/en
Publication of WO1994026934A2 publication Critical patent/WO1994026934A2/en
Publication of WO1994026934A3 publication Critical patent/WO1994026934A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A two-step nucleic acid hybridization probe assay for certain types of human papilloma virus (HPV) associated with cervical cell dysplasia and malignancy comprises a fluid phase capture hybridization step in which amplified specific gene E6/E7 messenger RNA from a biological specimen is hybridized to a biotinylated capture reagent to form a complex, attachment of the capture reagent complex to a solid phase by reaction with immobilized streptavidin, a second hybridization step in which a virus type-specific enzyme-conjugated detection probe hybridizes with the complexed amplified messenger RNA, and detection of the complexed detection probe by color or fluorophor production following a wash of the solid phase and addition of an appropriate chromogenic or fluorogenic substrate. The assay has enhanced sensitivity compared to conventional tests and is specific for actual expression of HPV oncogenes in cervical specimens, and not merely indicative of viral presence.
PCT/US1994/005085 1993-05-06 1994-05-06 Human papillomavirus detection assay WO1994026934A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP94917931A EP0655091A1 (en) 1993-05-06 1994-05-06 Human papillomavirus detection assay
CA002139623A CA2139623A1 (en) 1993-05-06 1994-05-06 Human papillomavirus detection assay
AU69456/94A AU6945694A (en) 1993-05-06 1994-05-06 Human papillomavirus detection assay
JP6525595A JPH07508891A (en) 1993-05-06 1994-05-06 Human papillary species virus detection assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5892093A 1993-05-06 1993-05-06
US08/058,920 1993-05-06

Publications (2)

Publication Number Publication Date
WO1994026934A2 WO1994026934A2 (en) 1994-11-24
WO1994026934A3 true WO1994026934A3 (en) 1995-01-26

Family

ID=22019729

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/005085 WO1994026934A2 (en) 1993-05-06 1994-05-06 Human papillomavirus detection assay

Country Status (5)

Country Link
EP (1) EP0655091A1 (en)
JP (1) JPH07508891A (en)
AU (1) AU6945694A (en)
CA (1) CA2139623A1 (en)
WO (1) WO1994026934A2 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19506561C1 (en) * 1995-02-24 1996-10-10 Deutsches Krebsforsch A method for the early detection of HPV-associated carcinomas or high-grade HPV-induced dysplasias
DE19526717A1 (en) * 1995-07-21 1997-01-23 Florian Dr Med Heirler Diagnosis of cervical cancer
FR2737502B1 (en) * 1995-07-31 1997-10-24 Genset Sa NUCLEIC ACID DETECTION METHOD USING NUCLEOTIDE PROBES ALLOWING BOTH SPECIFIC CAPTURE AND DETECTION
AU766744B2 (en) * 1995-11-15 2003-10-23 Gen-Probe Incorporated Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits
WO1997018334A2 (en) * 1995-11-15 1997-05-22 Gen-Probe Incorporated Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits
US6013488A (en) * 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
AU732703B2 (en) 1996-11-20 2001-04-26 Crucell Holland B.V. An improved method for the production and purification of adenoviral vectors
EP1187939B9 (en) * 1999-07-09 2009-08-12 Gen-Probe Incorporated Detection of hiv-1 by nucleic acid amplification
WO2001030993A1 (en) * 1999-10-25 2001-05-03 Wakunaga Pharmaceutical Co., Ltd. Method of detecting target nucleic acid
AU2001253471B2 (en) * 2000-04-13 2007-07-26 Medical University Of South Carolina Tissue-specific and pathogen-specific toxic agents, ribozymes, dnazymes and antisense oligonucleotides, and methods of use thereof
US6447995B1 (en) 2000-10-04 2002-09-10 Genvec, Inc. Utilizing intrinsic fluorescence to detect adenovirus
GB0120938D0 (en) * 2001-08-29 2001-10-17 Norchip As Detection of human papillomavirus E7 mRNA
ATE354677T1 (en) 2002-01-07 2007-03-15 Norchip As DETECTION METHOD FOR PAPILLOMAVIRUS MRNA
AU2005314061B2 (en) 2004-12-08 2010-01-28 Gen-Probe Incorporated Detection of nucleic acids from multiple types of human papillomaviruses
WO2006075245A2 (en) 2005-01-14 2006-07-20 The Regents Of The University Of Michigan Systems, methods, and compositions for detection of human papilloma virus in biological samples
US7524631B2 (en) 2005-02-02 2009-04-28 Patterson Bruce K HPV E6, E7 mRNA assay and methods of use thereof
US20070111960A1 (en) * 2005-03-04 2007-05-17 Advandx, Inc. High affinity probes for analysis of human papillomavirus expression
GB0701253D0 (en) * 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
CA2785529C (en) 2010-02-11 2019-01-08 Nanostring Technologies, Inc. Compositions and methods for the detection of small rnas
WO2012054727A1 (en) 2010-10-22 2012-04-26 Bio-Rad Laboratories, Inc. Reverse transcriptase mixtures with improved storage stability
AU2013205122B2 (en) 2012-10-11 2016-11-10 Gen-Probe Incorporated Compositions and Methods for Detecting Human Papillomavirus Nucleic Acid
WO2018059581A1 (en) * 2016-09-30 2018-04-05 广州易活生物科技有限公司 Probe for hpv virus genotyping detection based on efirm technique, kit and use
WO2022067023A1 (en) * 2020-09-24 2022-03-31 Mayo Foundation For Medical Education And Research Screening platforms

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0373352A2 (en) * 1988-11-11 1990-06-20 BEHRINGWERKE Aktiengesellschaft Method of detecting spliced mRNA
EP0420260A2 (en) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Biotin-labelled DNA by polymerase chain reaction and detection thereof
EP0425217A2 (en) * 1989-10-23 1991-05-02 Ciba Corning Diagnostics Corp. A hybridization assay for campylobacter rRNA
WO1991008312A1 (en) * 1989-12-01 1991-06-13 Gene-Trak Systems Detection of hpv transcripts
WO1991019812A1 (en) * 1990-06-11 1991-12-26 Bio Merieux Method for detecting a nucleotide sequence by sandwich hybridization
WO1992001815A1 (en) * 1990-07-19 1992-02-06 Royal Free Hospital School Of Medicine Method for the diagnosis of human pappiloma virus type 16 using the polymerase chain reaction
WO1992014847A2 (en) * 1991-02-13 1992-09-03 Orgenics International Holdings B.V. Detection of high risk and low risk human papillomavirus by enzymatic amplification of dna
WO1993024658A1 (en) * 1992-05-29 1993-12-09 Gen Trak, Inc. Signal amplification probe and methods of use

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0373352A2 (en) * 1988-11-11 1990-06-20 BEHRINGWERKE Aktiengesellschaft Method of detecting spliced mRNA
EP0420260A2 (en) * 1989-09-29 1991-04-03 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Biotin-labelled DNA by polymerase chain reaction and detection thereof
EP0425217A2 (en) * 1989-10-23 1991-05-02 Ciba Corning Diagnostics Corp. A hybridization assay for campylobacter rRNA
WO1991008312A1 (en) * 1989-12-01 1991-06-13 Gene-Trak Systems Detection of hpv transcripts
WO1991019812A1 (en) * 1990-06-11 1991-12-26 Bio Merieux Method for detecting a nucleotide sequence by sandwich hybridization
WO1992001815A1 (en) * 1990-07-19 1992-02-06 Royal Free Hospital School Of Medicine Method for the diagnosis of human pappiloma virus type 16 using the polymerase chain reaction
WO1992014847A2 (en) * 1991-02-13 1992-09-03 Orgenics International Holdings B.V. Detection of high risk and low risk human papillomavirus by enzymatic amplification of dna
WO1993024658A1 (en) * 1992-05-29 1993-12-09 Gen Trak, Inc. Signal amplification probe and methods of use

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J.T. BROWN AND A.T. WORTMAN: "Rapid amplification of human Papillomavirus type 16 and 18 E6 and E7 mRNA by 3SR", DIAGNOSTIC MOLECULAR BIOLOGY: PRINCIPLES AND APPLICATIONS, PERSING ET AL. (ED.), 0(0), 13 May 1993 (1993-05-13), XXII+641P. AMERICAN SOCIETY FOR MICROBIOLOGY (ASM): WASHINGTON, DC,US;, pages 414 - 419 *
J.T. BROWN AND E. W. RADANY: "Development of a rapid assay for the detection of HPV transforming gene expression", ICAA, PROGRAM ABSTRACT, vol. 31, no. 0, 1991, pages 334 *
J.T. BROWN ET AL.: "Isothermal enzymatic amplification of HPV RNA using the 3SR reaction", J. CELL. BIOCHEM. SUPPL. VOL.111, NO. 5 (PART 2),, November 1990 (1990-11-01), ROCKEFELLER UNIV. PRESS, N.Y., US;, pages 56A *
T.R. GINGERAS ET AL.: "Unique features of the self-sustained sequence replication (3SR) reaction in the in vitro amplification of nucleic acids", ANNALES DE BIOLOGIE CLINIQUE, vol. 48, 1990, PARIS, FR;, pages 498 - 501 *

Also Published As

Publication number Publication date
CA2139623A1 (en) 1994-11-24
AU6945694A (en) 1994-12-12
WO1994026934A2 (en) 1994-11-24
EP0655091A1 (en) 1995-05-31
JPH07508891A (en) 1995-10-05

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