KR20140018589A - Antimicrobial composition comprising natural plant extract - Google Patents

Abstract

The present invention relates to an antimicrobial composition comprising a natural plant-derived extract as an active ingredient, and more particularly, to an antimicrobial composition containing a hyacinth, algae, seaweed or reed extract as an active ingredient. The extract of the present invention is excellent in antimicrobial activity against various pathogenic microorganisms, non-toxic and excellent effect even in a small amount can be usefully used in various antibacterial agents, antifungal agents, food preservative additives, cosmetic additives and antifungal pesticides It works.

Description

Antimicrobial composition comprising natural plant extract as an active ingredient

The present invention relates to an antimicrobial composition comprising extracts of natural plants, reeds, reeds, hyacinths or scabies as active ingredients.

In general, the direct or indirect damage caused by pathogenic microorganisms causes many economic, environmental and medical problems. In particular, losses due to corruption in the food distribution process in the food industry, the harmful effects of humans and environmental pollution from the use of excessive chemical pesticides on crops in the agricultural industry, and the appearance of antibiotic-resistant strains due to the misuse of antibiotics It becomes a chronic problem in the first half. In this reality, natural bioactive substances isolated from plants exhibit antibiotic activity in a wide range of pathogenic bacteria, fungi, etc., but development of new alternatives is urgent in that they can be used environmentally.

On the other hand, antibiotics have a selective toxicity effect that kills microorganisms and is low in humans or animals and is not inactivated by enzymes in the body, such as DNA replication, transcription and translation of genetic information, and electrons. It inhibits the transport of energy, the biosynthesis of the cell wall, and the like through the mechanism of inhibiting the growth of microorganisms.

The antibiotic was first discovered by Pen Alexinor Fleming in 1929 and by Flory et al. In 1939 for the first time to isolate and purify penicillin from a culture of penicillium chrysogenum. It has been known as a miracle medicine by saving the lives of numerous patients with it. Since 1959, full-fledged research has developed benzylpenicillin, streptomycin, tetracycline, and macrolide erythromycin, which have been used as antibiotics to date. .

However, soon after penicillin was used, resistant bacteria began to appear, and most antibiotics not only have multiple chiral centers but also have complex structures such as macrolides or peptides. Not only this difficulty, but also the synthesis method has a problem of very low economical compared to the fermentation method.

Therefore, it is urgent to find new antimicrobial substances having excellent efficacy from new natural products.

Korean Laid-Open Patent No. 2012-0081365

Therefore, an object of the present invention is to provide an antimicrobial composition comprising a natural plant extract having excellent antibacterial activity as an active ingredient without any side effects on the living body.

In order to achieve the object of the present invention as described above, the present invention provides an antimicrobial composition comprising as an active ingredient at least one extract selected from the group consisting of hyacinth, ulji, hamcho and reeds.

In one embodiment of the present invention, the extract may be water, an organic solvent or a mixed solvent extract thereof.

In one embodiment of the present invention, the organic solvent may be selected from the group consisting of methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol) and butanol (butanol).

In one embodiment of the present invention, the extract may be included in 0.1 to 95% by weight relative to the total weight of the composition.

In one embodiment of the present invention, the extract may be one organic solvent extract selected from the group consisting of stems, leaves, roots, flowers and bulbs of hyacinth, sage, vinegar or reeds.

In one embodiment of the present invention, the extract is Staphylococcus epidermidis (KCCM 35494 epidermal staphylococci), Staphylococcus aureus (KCCM 11335 Staphylococcus aureus), Pityrosporum ovale (KCCM Seborrheic Dermatitis), Listeria monocytogenes (KCCM low temperature food poisoning bacteria), Bacillus subtilis (KCCM 11316 Cheonggukjang Fermented Bacteria), Corynebacterium ammoniagenes (KCCM 1174 Ammonia Synthesis), and Candida albicans (KCCM oralitis, gastroenteritis-producing fungi) may have activity to inhibit one or more strains selected from the group consisting of.

In one embodiment of the present invention, the extract may have an activity of inhibiting the growth of porcine epidemic diarrhea virus (PEDV).

The present invention relates to an antimicrobial composition comprising a natural plant-derived extract as an active ingredient, and more particularly, to an antimicrobial composition containing a hyacinth, algae, seaweed or reed extract as an active ingredient. The extract of the present invention is excellent in antimicrobial activity against various pathogenic microorganisms, non-toxic and excellent effect even in a small amount can be usefully used in various antibacterial agents, antifungal agents, food preservative additives, cosmetic additives and antifungal pesticides It works.

The present invention is characterized in that it provides an antimicrobial composition comprising as an active ingredient at least one extract selected from the group consisting of hyacinth, algae, seaweed and reed.

`` Corylopsis coreana ) is an evergreen family of 27 genera and 140 species worldwide, mainly distributed in East Asia, and rarely in North and Central America. There are 29 species of genus locusts in the locust family. There are 5 species in Japan, 5 species in India, and 1 species in Korea. Korean hyacinth is deciduous broad-leaved shrub, scientific name Corylopsis coreana Uyeki and the name is korean winter hazel . Korea's hyacinth was found in Songgwangsa, Suncheon, Jeonnam, in 1910. It is a special plant and registered in 1924 in academia. These hyacinths are deciduous broad-leaved shrubs that grow to 1 to 5m, forming clusters, and many neighboring children rise up to form large groups, which grow together with azaleas, jinrae, sesame seeds, red beans and sage trees, and grow well in sunny places. It is strong and does not suffer from the East Sea even below minus 30 degrees. It is also known to grow well in dry soil due to its strong dry resistance.

' Erythronium japonicum ) is a lily and perennial herb that is a monocotyledonous plant in the flowering plant, also called Chajeon Fosanjago, crayfish, algae, erage, algae and silk herb, and the scientific name is Erythronium japonicum ( Balrer ) Decne . It grows in a group of alpine sunny forests, and it takes more than 7 years to grow from seed to flower and blooms in March-May. This leans back completely. The scaly stem is fleshy on white, and several pieces are extended laterally in the ground, and the flower stem stands straight at the end of the scaly stem and leaves 1 ~ 2 sheets. There are about 24 species around the world, and rarely white flowers, which may be due to recessive genes. These stalks are boiled from spring leaves, dried in shade and eaten as herbs, and medicinal scales are medicinal. Gastrointestinal function to improve the action to stop vomiting and diarrhea, headaches and dizziness is also known to be effective.

' Salicornia herbacea ) 'is a dicotyledonous plant that grows on the beach as a yearly herbaceous plant of the main tree, the stem is many and prominent, split 1-2 times, and 2-3 small flowers are hidden in the hollows of the nodes. The husk is inverted conical fleshy, grows after pinching and surrounds the fruit. It is distributed in Korea, Japan, China, India, and North America. In Korea, it is a year-old herb that absorbs and grows seawater on the west coast and the tidal-flat coast. These herbs are known to have effects such as diabetic improvement, constipation, atopy, blood purifying control, elimination of stool, and improvement of sexual function.

Phragmites communis ) 'is a monocotyledonous plant with a rice paddy crest. It is a perennial herbaceous plant with a height of 3m and its roots are coarse, large, long, and have many beard roots in the node and yellowish white. Stems are long, large, cylindrical, hard, gathered, and stand upright. Leaves are alternate in 2 lines, narrow, 50cm long, 4cm wide and wide, with sharp tips, rough edges, and green. The leaf is not hairless, the tongue is short, and the hair is on the edge. Flowers are conical inflorescences, large, 15-50cm long, end of stem, many branches are split, many small spikes are in close contact, first purple, and later purple. The small spikes are 5 flowers, long, thin, and sharp. Marsh grows in swamps, mudflats, and sandy areas around lakes, and is distributed in temperate, temperate, and subtropical regions of Korea and the northern hemisphere.

The inventors have found that the extracts of the plants described above, such as hyacinth, sage, seaweed or reed have excellent antimicrobial activity, the extract according to the present invention from the nature using the extraction and separation methods known in the art Extraction and separation can be used, and the 'extract' as defined in the present invention is extracted from hyacinth, sage, seaweed or reed plants using a suitable solvent, for example, crude extract, polar solvent soluble in the plant It includes both extracts or nonpolar solvent soluble extracts. In addition, the plant may specifically be a whole plant, an extract extracted from the stem, leaves, flowers, roots, bulbs of the plant.

As a solvent that can be used for the extract, water, an organic solvent or a mixed solvent thereof may be used, but is not limited thereto, but may be purified water, methanol, ethanol, propanol or isopropanol. Alcohol containing 1 to 4 carbon atoms, butanol, etc., acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride , Various solvents such as hexane and cyclohexane can be used alone or in combination.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method.

In addition, the extract obtained from the hyacinth, ulji, seaweed or reeds included in the composition of the present invention is the powder obtained by the additional process such as distillation under reduced pressure and freeze drying or spray drying, extracted by hot water extraction or solvent extraction method described above. It can manufacture in a state. Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, the extract obtained from hyacinth, sage, seaweed or reed is a concept including all extracts, fractions and purified products obtained at each step of extraction, fractionation or purification, their dilutions, concentrates or dried products.

In addition, the extract of the present invention may be included in a concentration of 10 to 100,000 ppm in the antimicrobial composition of the present invention, the extract of the present invention may also be included in 0.1 to 95% by weight relative to the total weight of the composition.

On the other hand, the antimicrobial composition according to the present invention is a harmful strain Staphylococcus epidermidis (KCCM 35494 epidermal staphylococci), Staphylococcus aureus (KCCM 11335 Staphylococcus aureus), Pityrosporum ovale (KCCM Seborrheic Dermatitis), Listeria monocytogenes (KCCM low temperature food poisoning bacteria), Bacillus subtilis (KCCM 11316 Cheonggukjang Fermented Bacteria), Corynebacterium ammoniagenes (KCCM 1174 ammonia-synthesizing bacteria) and Candida albicans (KCCM oralitis, gastroenteritis-producing fungi) and have the activity of inhibiting one or more strains selected from the group, and also epidemic diarrhea virus (porcime epidemic diarrhea virus; PEDV). ) Inhibits proliferation.

Therefore, the antimicrobial composition comprising one or more extracts selected from the group consisting of hyacinth, algae, seaweed and reeds according to the present invention as an active ingredient has excellent antimicrobial activity against various pathogenic microorganisms, and is non-toxic and effective even in trace amounts. It is excellent in various antibacterial agents, antifungal agents, food preservative additives, cosmetic additives and antifungal pesticides can be usefully used.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  1>

Preparation of Methanol and Ethanol Extracts by Plants

Stems and leaves of seaweed, roots and leaves of reeds, flowers, stems, leaves, flowers of flowers, leaves, and bulbs of hyacinth are dried at room temperature and crushed with a pulverizer. Each of them was added, and the mixture was immersed for 4 weeks, filtered, and each extractive solvent was dried using a rotary low pressure vacuum concentrator, and then the lyophilized extract was used in the following experiment.

< Experimental Example  1>

Antimicrobial Activity

The antimicrobial activity of the harmful strains described in Table 1 was investigated in the methanol and ethanol extracts of the parts of each plant obtained in Example 1. Antimicrobial activity analysis was determined by disc agar medium diffusion (Bauer et al., 1996). That is, each extract having a concentration of 5, 10, 15, 20 mg · mL ⁻¹ was filtered through a 0.45 μm membrane filter (Millipore Co., USA) to sterilize, and then sterilized filter paper disc filter paper disc (8 mm, Whatman, USA). 50μl), and extract solvents are aseptically air-dried to remove them completely, and each bacteria are incubated at growth temperature for 24 hours, and the diameter of the transparent low zone around the paper disc is measured to determine the antimicrobial activity. Compared.

As a result, methanol extract was found to be Staphylococcus epidermidis (epidermal staphylococci), S taphylococcus aureus (yellow staphylococcus aureus), Pityrosporum ovale (seborrheic dermatitis), Listeria monocytogenes (thermic food poisoning bacterium ), Bacillus subtilis (Cheongkukjang fermented bacteria), Corynebacterium ammoniagenes , Candida All treatments of albicans (oral infection, gastroenteritis-producing fungi) began to exhibit antimicrobial activity at 15 μl concentration and the best antibacterial activity at 20μl treatment. Especially Corynebacterium Antimicrobial activity against ammoniagenes and Candida albicans was good (see Tables 2 to 5 below).

Ethanol Extract Staphylococcus epidermidis , Pityrosporum The ovale and Candida albicans treatments showed antimicrobial activity at the concentration of 10µl. Especially Pityrosporum The antimicrobial activity of ovale and Candida albicans was relatively good. This phenomenon was similar in the stem treatment group and the stem treatment group showed better antibacterial activity than the leaf treatment group.

In conclusion, the antimicrobial activity was good in the methanol and ethanol treatments, but the higher the concentration of the extract, the higher the activity and the better antibacterial activity in the methanol solvent treatment treatment (see Tables 2 to 5 below).

In the case of reeds, as shown in Tables 6 to 9 below, most of the methanol extracts of the roots and leaves showed antimicrobial activity from 15 µl of the addition concentration of the extracts, and Staphylococcus epidermidis , Bacillus As antimicrobial activity increased to 20μl against subtilis and Candida albicans , all antibacterial treatments showed good activity.

In conclusion, the antimicrobial activity was good in the methanol and ethanol treatments, but the higher the concentration of the extract, the higher the activity.

In the case of hydroxya, as shown in Tables 10 to 15 below, methanol extracts showed the antimicrobial activity from 15 μl treatment of most extracts, and the highest antibacterial activity of Staphylococcus epidermidis treatment It was active, and was found to be S taphylococcus aureus, Bacillus subtilis, Corynebacterium ammoniagenes , Candida The albicans treatment showed good activity, and the antimicrobial activity was the best at 20µl treatment. Ethanol treatment showed slightly lower activity than methanol treatment, but antibacterial activity occurred in all treatments.

In the case of Algeol, as shown in Table 16 to Table 21, the methanol extract began to show antimicrobial activity at the concentration of 10 μl treatment and showed most antibacterial activity at 20 μl treatment and Bacillus subtilis , Candida Albicans treatment showed good activity, and antimicrobial activity was good at 20µl treatment. In the case of ethanol treatment, the activity was slightly lower than that in methanol treatment, but the reaction occurred in all treatments.

In conclusion, the antimicrobial activity of the larvae was in the order of bulb> flower> leaf.

< Experimental Example  2>

Antiviral Measurement

<2-1> Sample Preparation

Stems and leaves of the grasswort, roots and leaves of reeds, flowers, stems, leaves, and flowers of hyacinths, stems, leaves, and bulbs are dried at room temperature and pulverized with a grinder, followed by adding methanol and ethanol at 100 times the sample weight. After immersion for a day and filtered, the extraction solvent was dried using a rotary low pressure vacuum concentrator and then lyophilized to be used for the experiment.

<2-2> Virus Culture

The virus used to measure the antivirality of each extract against PEDV was porcine epidemic diarrhea virus (PEDV) and cultured in Vero cells. In addition, a minimal essential media medium containing 10% of pediatric serum was used.

<2-3> virus inhibition assay

Vero cells were cultured in 96 well plates to measure the virus proliferation of the plants, reeds, hyacinths and larvae against PEDV, and when the cells were at the bottom of 90% of the wells, the existing cultures were removed and PEDV was included. New cultures were administered to each well. The concentration of each plant extract was 200, 400, 800 ㎍ / ㎖ administered to the well. The virus proliferation inhibitory activity of each plant was measured by SRB assay (Martin & Martin, 1997) after infecting PEDV with vero cells for 48 hours.

As a result of the analysis, as shown in Tables 22 to 25, in the case of seaweed, the response was somewhat better than the leaf extract in the cycle, and the higher the concentration, the better the antiviral activity. In addition, methanol extract showed better antiviral response than ethanol extract, and this phenomenon was similar in reed treatment. In the case of reeds, the antiviral effect was good in the roots, and methanol extraction showed better response.

In the case of hydroxya, antiviral phenomena appeared in the order of flowers>stems> leaves, and methanol extracts showed better response. The higher the concentration, the better the antiviral response. This phenomenon was similar in the case of ulge, and showed the antiviral response in the order of structure>leaf> flower.

So far I looked at the center of the preferred embodiment for the present invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (7)

Antimicrobial composition comprising one or more extracts selected from the group consisting of hirley, ulji, seaweed and reed as an active ingredient. The method of claim 1,
The extract is an antimicrobial composition, characterized in that water, an organic solvent or a mixed solvent extract thereof. 3. The method of claim 2,
The organic solvent is antimicrobial composition, characterized in that selected from the group consisting of methanol (methanol), ethanol (ethanol), propanol (propanol), isopropanol (isopropanol) and butanol (butanol). The method of claim 1,
The extract is an antimicrobial composition, characterized in that contained in 0.1 to 95% by weight based on the total weight of the composition. The method of claim 1,
The extract is an antimicrobial composition, characterized in that the extract of one organic solvent selected from the group consisting of stems, leaves, roots, flowers and bulbs of hyacinth, sage, seaweed or reeds. The method of claim 1,
The extract is Staphylococcus epidermidis (KCCM 35494 epidermal staphylococci), Staphylococcus aureus (KCCM 11335 Staphylococcus aureus ), Pityrosporum ovale (KCCM seborrheic dermatitis), Listeria monocytogenes (KCCM low temperature food poisoning bacteria), Bacillus subtilis (KCCM 11316 Cheonggukjang Fermented Bacteria), Corynebacterium ammoniagenes (KCCM 1174 Ammonia Synthesis), and Candida Albicans (KCCM oralitis, gastroenteritis-producing fungi) Antimicrobial composition characterized in that it has the activity of inhibiting one or more strains selected from the group consisting of. The method of claim 1,
The extract is antimicrobial composition characterized in that it has an activity of inhibiting the growth of porcine epidemic diarrhea virus (PEDV).