KR20130019725A - A pharmaceutical composition comprising the fractions of water extract from curcuma longa l. for preventing alcoholic liver damage - Google Patents

Abstract

PURPOSE: A pharmaceutical composition containing a fraction of a Curcuma Longa L. extract for preventing alcoholic liver damage is provided to suppress generation of alcoholic lipid peroxide and to enhance antioxidation. CONSTITUTION: A pharmaceutical composition for preventing alcoholic liver damage contains a fraction of a Curcuma Longa L. water extract as an active ingredient. The fraction is prepared by extracting Curcuma Longa L. with hot water and fractioning with chloroform. The liver damage is alcoholic fatty liver, alcoholic hepatitis, or alcoholic cirrhosis. A health functional food for preventing alcoholic liver damage contains the fraction as an active ingredient.

Description

A Pharmaceutical Composition Comprising The Fractions Of Water Extract From Curcuma Longa L. For Preventing Alcoholic Liver Damage}

The present invention relates to a pharmaceutical composition for preventing alcoholic liver damage comprising a fraction of turmeric water extract as an active ingredient, and more particularly, to a chloroform or ethyl acetate fraction of the turmeric water extract of the present invention as an active ingredient. It relates to a pharmaceutical composition for preventing alcoholic liver injury.

Curcuma Longa L. is a perennial herbaceous plant belonging to the Zinigiberaceae, which grows in hot and humid southern Asia, Africa, and Latin America. In Korea, Eulgeum, Gumgeum, and Geumum (玉) It is also called 金, 金 金, and turmeric, and is cultivated in all parts of the country except the mountainous region of the north. The main component of turmeric is curcuminoid, which contains the highest amount of curcumin, and p-hydroxycinnamoylferuloylmethane and p, p'-dihydride. It contains a yellow pigment of p'-dihydroxydicinnamoylmethane. The pharmacological effects of turmeric reported to date include gastric juice secretion, diuretic and anticancer effects. Curcumin contained in turmeric is known to have antimicrobial, anti-tumor, anti-amyloid and anti-inflammatory effects.

Meanwhile, the liver is a very important organ that is responsible for various metabolism, detoxification, decomposition, synthesis and secretion in our body. First, the liver has the ability to manage energy metabolism, which metabolizes all the nutrients absorbed by food into energy-producing substances that are supplied or stored throughout the body. Second, the liver has the function of synthesizing, storing and distributing fats such as serum proteins, bile acids, phospholipids and cholesterol of about 2,000 enzymes, albumin and coagulation factors. Third, the liver has the ability to excrete various metabolites into the duodenum through the bile ducts, and the immune function plays an important role in maintaining our lives. Finally, the liver detoxifies and breaks down drugs, toxicants, and alcohol. However, the liver detoxification function is easy to damage liver cells can cause drug, toxic, alcoholic liver diseases.

Alcoholic liver disease can be divided into alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis according to clinical symptoms, and it usually occurs when people drink 60-80g of alcohol a day for 10 years. Alcoholic fatty liver is caused by the accumulation of cholesterol and triglycerides in the liver cells due to excessive alcohol intake, which can be recovered soon after drinking, but will continue to develop hepatitis if continued drinking. Alcoholic hepatitis is a condition of necrosis and inflammation of hepatocytes, and shows various symptoms such as fatigue, anorexia, weight loss, jaundice, fever, and right upper abdominal pain, and about 40% of patients with alcoholic liver cirrhosis develop. Alcoholic cirrhosis is a condition that is impossible to recover from normal liver, with various symptoms such as systemic fatigue, loss of appetite, ascites, esophageal varices, bleeding, hepatic encephalopathy, lethargy, and poor prognosis than cirrhosis caused by hepatitis virus. In Esau, 50% of deaths from end-stage liver disease are known to be caused by alcohol.

In order to reduce the alcoholic liver disease mortality as described above, there is an urgent need to develop alcoholic liver injury prevention and treatment, but until now, no appropriate therapeutic agent, health functional food, and related composition for treating alcoholic liver injury have been developed.

An object of the present invention is to provide a pharmaceutical composition and health functional food for preventing alcoholic liver damage containing a fraction of turmeric water extract as an active ingredient.

In order to achieve the above object, one aspect of the present invention provides a pharmaceutical composition for preventing alcoholic liver damage containing a fraction of turmeric water extract as an active ingredient.

In addition, another aspect of the present invention to achieve the above object provides a dietary supplement for preventing alcoholic liver damage containing a fraction of turmeric water extract as an active ingredient.

Fractions of turmeric water extract of the present invention inhibits the damage of liver cells by alcohol, inhibits the production of alcoholic lipid peroxides, and increases the antioxidant activity, the drug or health functional food for the prevention of alcoholic liver damage It can be usefully used as.

However, the effects of the present invention are not limited to the above-mentioned effects, and other effects not mentioned will be clearly understood by those skilled in the art from the following description.

Figure 1 is a diagram showing the results of experiments confirming the effect of hepatic cell damage caused by alcohol of turmeric water extract in HepG2 / 2E1 cells.
2 is a graph showing the results of experiments confirming the inhibitory effect of alcohol on the ALT activity of turmeric water extract in male C57BL / 6 mice.
Figure 3 is a graph showing the results of experiments comparing the effect of liver water damage by turmeric water extract, chloroform fraction and ethyl acetate in HepG2 / 2E1 cells.
Figure 4 is a diagram showing the results of experiments confirming the effect of preventing chloroform and ethyl acetate fraction of the liver water damage caused by alcohol of turmeric water extract in HepG2 / 2E1 cells.
5 is a graph showing the experimental results confirming the inhibitory effect of lipid peroxide accumulation by alcohol of the chloroform fraction of turmeric water extract in HepG2 / 2E1 cells.
6 is a graph showing the results of experiments confirming the antioxidant activity of the chloroform fraction of turmeric water extract.

Hereinafter, the present invention will be described in detail.

The present invention provides a pharmaceutical composition for preventing alcoholic liver damage, which contains a fraction of Curcuma Longa L. water extract as an active ingredient.

In addition, the present invention provides a functional food for preventing alcoholic liver damage containing a fraction of Curcuma Longa L. water extract as an active ingredient.

In the composition or dietary supplement, alcoholic liver damage is preferably alcoholic fatty liver, alcoholic hepatitis or alcoholic cirrhosis, but is not limited thereto.

In the composition or health functional food, the fraction may be prepared by hot water extraction of turmeric, fractionated with chloroform, the chloroform fraction is separated and the remaining solution may be prepared by fractionation again with ethyl acetate. More specifically, the fraction is 1) immersed semi-dried turmeric in 5 to 20 times the total weight of the extraction solvent, and extracted for 1 to 12 hours at a temperature of 50 ℃ to 250 ℃; 2) filtering the extract of step 1); And 3) fractionating the filtered extract of step 2) into 1 to 3 volumes of chloroform. However, the present invention is not limited thereto.

In the above method, the extraction solvent of step 1) may be any one of water, alcohol or a mixture thereof, and the extraction solvent is more preferably water, but is not limited thereto. The extraction solvent is preferably extracted by adding 5 to 20 times the total weight of turmeric, more preferably 7 to 15 times the total weight of turmeric, and 10 times of the total weight of turmeric Most preferably, but not limited thereto. The extraction temperature of step 1) is preferably 50 ° C to 250 ° C, more preferably 70 ° C to 200 ° C, and most preferably 100 ° C to prevent rot, discoloration and odor. However, the present invention is not limited thereto. The extraction time of step 1) is preferably 1 to 12 hours, more preferably 3 to 10 hours, and most preferably 5 hours, but is not limited thereto. Extraction of the step 1) may be used in the extraction method commonly used in the art, such as hot water extraction, bath extraction, ultrasonic extraction, Soxhlet extraction and reflux cooling extraction. In the above method, the filtration of step 2) may be used all the filtration method commonly used in the art. In particular, when the filtration of step 2) is carried out using a filtering network, the filtration is preferably filtered through a 50 to 200 mesh filter network, the filtration is more preferably filtered through a 70 to 150 mesh filter network, the filtration is Filtration with a 100 mesh filter net is most preferred, but not limited thereto. In the method, the chloroform of step 3) is preferably 1 to 3 times the volume of the extract filtered in step 2), more preferably 1.5 to 2.5 times the volume, most preferably 2 times the volume, It is not limited to this. In the above method, the fractionation method may further include the step of 4) separating the chloroform fraction of step 3), and then fractionating the remaining fraction again with 1 to 3 volumes of ethyl acetate, but is not limited thereto. . The ethyl acetate of step 4) is preferably 1 to 3 times the volume of the remaining fraction after separating the chloroform fraction of step 3), more preferably 1.5 to 2.5 times the volume, most preferably 2 times the volume. This is not limitative.

Fraction of turmeric water extract according to the present invention is effective in the prevention of alcoholic liver damage by inhibiting the damage of hepatocytes by alcohol in HepG2 / 2E1 cells, in particular chloroform fraction shows the most excellent effect among the fractions (Fig. 3 and Fig. 4). Moreover, the chloroform fraction of the turmeric water extract has the effect of inhibiting the production of alcoholic lipid peroxides in HepG2 / 2E1 cells (see FIG. 5) and increasing antioxidant activity (see FIG. 6). Therefore, the fraction of turmeric water extract may be usefully used as a pharmaceutical composition or health functional food for preventing alcoholic liver damage.

The pharmaceutical composition may further contain at least one active ingredient having the same or similar function in the fraction of turmeric water extract, and additionally include appropriate carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions. Can be. The carrier, excipient and diluent are lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol , Starch, gum Arabic, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, undetermined Vaginal cellulose, polyvinyl pyrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate stearate) and mineral oil, but are not limited thereto. When the pharmaceutical composition is formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants, etc. which are generally used may be used, but are not limited thereto.

The pharmaceutical composition may be administered by various routes such as oral, rectal, intravenous, intramuscular, subcutaneous, intrauterine dural or cerebrovascular, preferably orally administered. The pharmaceutical composition may be formulated for oral administration such as powders, granules, tablets, capsules, suspensions, or parenteral dosage forms such as ointments, external preparations, suppositories, and sterile injectable solutions, depending on the route of administration during actual clinical administration. Can be formulated and used. Solid preparations for oral administration may be prepared by mixing at least one excipient such as calcium carbonate, sucrose, lactose or gelatin in the fraction. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral administration may be prepared by mixing at least one diluent or excipient such as wetting agents, sweeteners, fragrances, preservatives and the like in the fractions. Preparations for parenteral administration may be prepared by mixing a sterile aqueous solution, a non-aqueous solvent, a suspending agent, an emulsion, a lyophilizer, and the like. As the non-aqueous solvent or suspending agent, propylene glycol, polyethylene glycol, and olive oil may be used. vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like can be used. In particular, when formulated as suppositories, witepsol, polyethylene glycol, tween 61, cacao butter, laurin butter, glycerogelatin, and the like may be used as the substrate. .

Preferred dosages of the pharmaceutical compositions vary depending on the age, condition, weight, degree of disease, type of drug, route of administration, and time of the patient. Generally, the dosage of the active ingredient for the desired effect is 0.001 to 1 The amount of mg / kg, preferably 0.001 to 0.1mg / kg is to be administered, it can be divided into several times a day, preferably 1 to 6 times at regular intervals according to the judgment of the doctor or pharmacist.

The health functional food may be provided in admixture with a food acceptable carrier, the type of food provided is not particularly limited. Examples of the food to which the fraction of the turmeric water extract can be added include meat, sausage, bread, chocolate, candy, snacks, confectionary, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, drinks , Tea, drink, alcoholic beverages and vitamin complexes, and the like, and may be provided in the form of powders, granules, tablets, capsules or beverages. When formulated as a beverage, the liquid component added in addition to the fraction of turmeric water extract may contain various flavors or natural carbohydrates as additional components, such as conventional beverages, but is not limited thereto. Examples of such natural carbohydrates include monosaccharides (e.g. glucose, fructose, etc.), disaccharides (e.g. maltose, sucrose, etc.) and polysaccharides (e.g. conventional sugars such as dextrin, cyclodextrin, etc.), and xylitol, Sugar alcohols such as sorbitol and erythritol. The proportion of such natural carbohydrates is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention. As flavoring agents other than those mentioned above, natural flavoring agents (taumarin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (e.g., saccharin, aspartame, etc.) can be used. . In another embodiment, the food composition of the present invention comprises various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic and natural flavors, colorants and enhancers (such as cheese, chocolate), pectic acid and salts thereof, organic acids , Protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The dietary supplement may also contain pulp for the production of fruit and vegetable beverages. These components may be used singly or in combination, and the proportion of such additives is generally selected in the range of 0.001 to 50 parts by weight based on the total weight of the composition.

Hereinafter, the present invention will be described in detail with reference to Examples and Production Examples.

However, the following Examples and Preparation Examples specifically illustrate the present invention, and the contents of the present invention are not limited by the following Examples and Preparation Examples.

Preparation of turmeric extract and fractions thereof

<1-1> Preparation of Water Extract of Turmeric

After dipping 40 kg of semi-dried Curcuma Longa L. (Jindo, Jeollanam-do, Korea) into 400 liters of purified water, use an industrial extractor (Myeongil E & C) for 5 hours at 100 ℃ and 750mmHg. After extraction, the resultant was filtered through a 100 mesh filter, concentrated under reduced pressure to 100 l at 55 to 60 ° C. and 15 mm Hg, and then lyophilized for 6 hours at 0.5 torr and −35 ° C. (LYOPH-PRIDE 100R, ( Note) Youngil ENG) to obtain a water extract of turmeric.

<1-2> Preparation of Fractions for Water Extracts of Turmeric

In <1-1>, 200 liters of chloroform was added to 100 liters of the water extract concentrated under reduced pressure, and the water layer and the chloroform layer were left to separate. After chemical equilibration, the chloroform layer was separated and concentrated under reduced pressure at 55 to 60 ° C. and 15 mmHg to remove all the solvent, and then dissolved and recovered in 1 ml of chloroform. The chloroform was removed by nitrogen gas to remove all the chloroform. Chloroform fraction (CLH-C) was obtained for the water extract.

In the process of preparing the chloroform fraction as described above, the chloroform layer was separated and 200 L of ethyl acetate was added to the remaining water layer, and the water layer and the ethyl acetate layer were left to separate. After chemical equilibration, the ethyl acetate layer was separated, concentrated under reduced pressure at 55 to 60 ° C. and 15 mm Hg to remove all solvents, and then dissolved and recovered in 1 ml of ethyl acetate. The total amount of chloroform was removed with nitrogen gas. This yielded an ethyl acetate fraction (CLH-E).

Measurement of liver damage prevention effect of water extract

<2-1> In Vitro  Experiment

XTT analysis was performed to confirm the alcoholic liver damage prevention effect of the water extract obtained in <1-1> of Example 1. Dispense 1 ml of MEM medium (Hyclone) into a HepG2 / 2E1 cell line (cells recombined by inserting the CYP2E1 gene into HepG2 (ATCC, Manassas VA USA) cells) at a density of 5 × 10 ⁴ / well in a 24-well plate. Incubated for 16 hours, followed by incubation for 24 hours at 37 ° C. for 5 days, exchanged with medium containing 15 μg / ml or 30 μg / ml water extract, 300 mM ethanol and 3% FBS. It was. On the 7th day, the extract, ethanol and FBS-containing medium were all removed and reacted for 2 hours by adding XTT-PMS solution (1 mg of XTT (sigma) and 100 mM PMS (sigma)). , Absorbance was measured at 450nm to confirm cell viability (BIO-TEK). In the negative control group, only 3% of FBS was cultured. Only the positive control group was cultured with a medium containing 300 mM ethanol and 3% FBS.

As a result, the positive control group showed 45% cell viability compared to the negative control group, while the experimental group cultured in the medium to which the 15 µg / ml water extract was added and the experimental group cultured in the medium to which the 30 µg / mL water extract was added. Showed cell viability of 65% and 80%, respectively (FIG. 1).

From the above results, it can be seen that the water extract of turmeric shows a prophylactic effect against alcoholic liver damage, and the effect of the water extract of turmeric shows a dose-dependent tendency.

<2-2> In Vivo  Experiment

Animal experiments were performed to determine the alcoholic liver damage prevention effect of the water extract obtained in <1-1> of Example 1. Thirty-two C57BL / 6 male mice (Oriental Bio, Gyeonggi-do, Korea) were illuminated for 7 days in a laboratory environment (temperature 23 ± 3 ° C, relative humidity 55 ± 15%, ventilation times 10-20 times / hr, 12-hour illumination Cycle, roughness 150 ~ 300Lux) to acclimatize and were fed with sufficient feed and water.

The 32 mice were divided into four groups (eight animals each) by appropriately distributing the same weights, and then the experiments were performed as shown in Table 1 below. The first group of mice was used as a negative control group by oral administration of distilled water only for 10 days, and the second group of mice was orally administered distilled water for 7 days, and then 5 g / kg / day on the 8th, 9th and 10th days. Distilled water dissolved in ethanol was orally administered and used as a positive control group. The mice of the experimental group 3 and 4 groups were orally administered distilled water dissolved in the turmeric water extract of 100 mg / kg / day and 500 mg / kg / day for 7 days, respectively, 8 days, 9 days On the 10th day, distilled water dissolved in 5g / kg / day ethanol and 100mg / kg / day water extract and distilled water dissolved in 5g / kg / day ethanol and 500mg / kg / day water extract were 12 hours apart. Orally administered five times. Six hours after the fifth dose, serum was obtained from each mouse and stored at −20 ° C. until the experiment.

1 to 7 days


8 days, 9 days, 10 days
(5 doses every 12 hours)

1st group (n = 8)

Distilled water

Distilled water

2nd group (n = 8)


Distilled water


Ethanol (5 g / kg / day) was added to distilled water.
Dissolve and administer


3rd group (n = 8)


In distilled water
Water extract (100mg / kg / day)
Dissolve and administer

Ethanol (5 g / kg / day) and
Water extract (100mg / kg / day)
Dissolve and administer


4th group (n = 8)


In distilled water
Water extract (500mg / kg / day)
Dissolve and administer

Ethanol (5 g / kg / day) and
Water extract (500mg / kg / day)
Dissolve and administer

As described above, the activity of ALT (alanine aminotransferase) in the serum obtained from each group of mice was measured and compared. As a result, the ALT activity was 13.62 IU / L in the negative control group 1, whereas the ALT activity was increased to 85.13 IU / L in the positive control group 2, and showed severe liver damage. On the other hand, mice in the third and fourth groups, which were administered the same with 5 g / kg / day of ethanol, were continuously administered the turmeric water extract during the administration of turmeric water extract in advance and 19.08, respectively. IU / L and 14.18 IU / L were found to show nearly the same / similar ALT activity as the negative control (FIG. 2).

From the above results, it can be seen that the water extract of turmeric shows a protective effect against alcoholic liver damage.

Measurement of liver damage prevention effect of fractions on water extract of turmeric

XTT analysis was performed to confirm the alcoholic liver damage prevention effect of the fractions obtained in <1-2> of Example 1. Dispense 1 ml of MEM medium (Hyclone) into a HepG2 / 2E1 cell line (cells recombined by inserting the CYP2E1 gene into HepG2 (ATCC, Manassas VA USA) cells) at a density of 5 × 10 ⁴ / well in a 24-well plate. After 16 hours of incubation, incubation at 37 ° C. for 24 hours was repeated for 5 days by exchanging with a medium containing 10 μg / ml of chloroform or ethyl acetate fraction, 300 mM ethanol and 3% FBS. On the 7th day, remove all the fractions, the medium containing ethanol and FBS, add XTT-PMS solution (1 mg of XTT (sigma) and 100 mM (sigma) mixed solution) and react for 2 hours. Absorbance was measured in order to confirm cell viability (BIO-TEK). In the negative control group, only 3% of FBS was cultured. Only the positive control group was cultured with a medium containing 300 mM ethanol and 3% FBS. In addition, in order to compare the efficacy of turmeric with water extract, the cell viability was also confirmed in the experimental group cultured in the medium containing the same amount of turmeric water extract.

As a result, the positive control group showed 40% cell viability compared to the negative control group, while the experimental group cultured in the medium containing the water extract, the experimental group cultured in the medium containing the chloroform fraction, and the culture medium containing the ethyl acetate fraction were added. One experimental group showed cell viability of 57%, 74% and 58%, respectively (FIG. 3).

In the same way, 50 μg / ml turmeric chloroform fractionation layer and turmeric ethyl acetate fractionation layer also showed 60% cell viability in the positive control group compared to the negative control group, while cultured in medium containing turmeric chloroform fraction. The experimental group and the experimental group cultured in the medium to which the ethyl acetate fraction was added showed cell viability of 92% and 69%, respectively (FIG. 4).

From the above results, it can be seen that the fraction of the water extract of turmeric shows a prophylactic effect against alcoholic liver damage, and it can be seen that the effect of the chloroform fraction is the most excellent among the fractions.

Inhibition of Lipid Peroxide Production by Chloroform Fraction

In order to measure the effect of inhibiting the formation of lipid peroxides of the chloroform fraction, which was found to have the best preventive effect against alcoholic liver damage in Example 3, the production concentration of malondialdehyde (MDA), a lipid peroxide, was measured. In order to measure the concentration of MDA, 1 × 10 ⁶ HepG2 / 2E1 cells were treated with 50 μg / ml of chloroform fractions and incubated for 3 days, and then the cells were harvested and harvested using passive lysis buffer (Promega). Was dissolved. The lysed cell lysate was centrifuged at 4 ° C. and 14000 rpm for 5 minutes to obtain a protein, and the obtained protein was quantified by the Bradford method. After adding 0.5 ml of quantified protein and 0.25 ml of 15% trichloroacetic acid (TCA) for 10 minutes at room temperature, centrifuging at 3000 rpm for 10 minutes, supernatant 0.5 ml and 0.37% TBA (thiobarbituric acid) 0.5 After the mixture was mixed and heated for 30 minutes in a 100 DEG C water bath, the reaction mixture was stopped in an ice water bath. Then, the supernatant was taken by centrifugation at 3000 rpm for 10 minutes and the absorbance was measured at 535 nm. In the negative control group, only 3% of FBS was cultured. Only the positive control group was cultured with a medium containing 300 mM ethanol and 3% FBS.

As a result, 200 nmol / mg MDA was detected in the negative control group and 390 nmol / mg MDA in the positive control group, and 280 nmol / mg MDA was detected in the experimental group treated with the chloroform fraction (FIG. 5).

From the above results, it can be seen that the chloroform fraction of the water extract of turmeric inhibits the production of lipid peroxide by alcohol, and it can be seen that alcoholic liver damage can be prevented through the inhibition of the production of lipid peroxide.

In order to measure the antioxidant activity of the chloroform fractions, 1 × 10 ⁶ HepG2 / 2E1 cells were treated with 50 μg / ml chloroform fractions and incubated for 3 days, after which the cells were harvested and passive lysis buffer (Promega). Cells were lysed using. The lysed cell lysate was centrifuged at 4 ° C. and 14000 rpm for 5 minutes to obtain a protein, and the protein obtained was quantified by Bradford method, followed by CAT (catalase) (Aebi et al., Method Enzymol. 105: 121-126), glutathion-S-transferase (GST) (Habig et al., Method Enzymol. 77: 398-405), superoxide dismutase (SOD) (McCord et al., Method (J. Biol. Chem. 244: 6049-6055), Glutathione peroxidase (GPx) (Thomson et al., Br. J. Nutr. 69: 577-588), Glutathion reductase (GR) (Worthington et al., Eur. J. Biochem. 67: 231-238) enzyme activity and reduced glutathion (GSH) (Akerboom et al., Method Enzymol. 77: 373-382) were measured in the negative control group cultured with only 3% FBS medium, In the positive control group, the culture medium was cultured with 300 mM ethanol and 3% FBS.

As a result, it was confirmed that the activity of the CAT, GST, SOD, GPx and GR enzymes and the concentration of reducing GSH was increased in the experimental group treated with the chloroform fraction compared to the positive control group (Fig. 6).

From the above results, it can be seen that the chloroform fraction of the water extract of turmeric has antioxidant activity, and it can be seen that alcoholic liver damage can be prevented due to such antioxidant activity.

Preparation Example 1 Preparation of Pharmaceutical Composition

<1-1> Preparation of Syrup

Syrup containing chloroform fraction of turmeric water extract as an active ingredient was prepared as shown in Table 2.

Ingredient Content (parts by weight) Chloroform Fraction of Example <1-2> 2 saccharin 0.8 Party 25.4 glycerin 8 Spices 0.04 ethanol 4 Sorbic acid 0.4 Distilled water 60

<1-2> Preparation of tablets

Tablets containing the chloroform fraction of turmeric water extract as an active ingredient were prepared as shown in Table 3.

Ingredient Content (parts by weight) Chloroform Fraction of Example <1-2> 250 Lactose 175.9 Potato starch 180 Colloidal silicic acid 32 10% gelatin solution 5 Potato starch 160 talc 50 Magnesium stearate 5

250 parts by weight of the chloroform fraction of Example <1-2>, 175.9 parts by weight of lactose, 180 parts by weight of potato starch and 32 parts by weight of colloidal silicic acid were mixed. 10% gelatin solution was added to the mixture, which was then ground and passed through a 14 mesh sieve. This was dried and the mixture obtained by adding 160 parts by weight of potato starch, 50 parts by weight of talc and 5 parts by weight of magnesium stearate was prepared as a tablet.

Production Example 2 Preparation of Food

Foods containing the chloroform fraction of turmeric water extract of the present invention were prepared as follows.

<2-1> Production of flour food

0.5 to 5.0 parts by weight of the chloroform fraction of Example <1-2> of the present invention was added to the flour, and bread, cake, cookies, crackers, and noodles were prepared using the mixture to prepare foods for health promotion.

<2-2> Production of Dairy Products

5 to 10 parts by weight of the chloroform fraction of Example <1-2> of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

<2-3> Preparation of Wire

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The chloroform fraction of Example <1-2> of the present invention was concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by spraying and drying with a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.

The dry powders of the chloroform fractions of the grains, seeds and turmeric water extracts prepared above were formulated in the following ratios.

(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

Chloroform fraction dry powder of Example <1-2> (3 parts by weight),

Ganoderma lucidum (0.5 parts by weight) and

(0.5 parts by weight)

As mentioned above, although preferred embodiment of this invention was described in detail, this invention is not limited to the said embodiment, A various deformation | transformation and a change are possible for those skilled in the art within the technical idea and range of this invention.

Claims (7)

A pharmaceutical composition for preventing alcoholic liver damage, which contains a fraction of Curcuma Longa L. water extract as an active ingredient. The pharmaceutical composition of claim 1, wherein the fraction is prepared by hydrothermal extraction of turmeric and fractionation with chloroform. The pharmaceutical composition of claim 1, wherein the fraction is prepared by hot water extraction of turmeric and fractionation with chloroform, and fractionation of the solution remaining after separation of the chloroform fraction with ethyl acetate. The pharmaceutical composition of claim 1, wherein the fraction is a chloroform fraction of turmeric water extract. The pharmaceutical composition of claim 1, wherein the liver injury is alcoholic fatty liver, alcoholic hepatitis, or alcoholic cirrhosis. Curcuma Longa L. A dietary supplement for the prevention of alcoholic liver damage, which contains a fraction of water extract as an active ingredient. 7. The dietary supplement for preventing alcoholic liver damage according to claim 6, wherein the fraction is a chloroform fraction of turmeric water extract.

Images