JPS5788358A - Method and apparatus for measuring urea - Google Patents
Method and apparatus for measuring ureaInfo
- Publication number
- JPS5788358A JPS5788358A JP55165597A JP16559780A JPS5788358A JP S5788358 A JPS5788358 A JP S5788358A JP 55165597 A JP55165597 A JP 55165597A JP 16559780 A JP16559780 A JP 16559780A JP S5788358 A JPS5788358 A JP S5788358A
- Authority
- JP
- Japan
- Prior art keywords
- solution
- measured
- urea
- gaseous
- room
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/58—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving urea or urease
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
PURPOSE:To determine urea quantitatively, by turning NH4<+> or NH3 produced by decomposing urea by urease enzyme into gaseous NH3 by adding a chemical reagent and after that, absorbing this gas in an acidic solution. CONSTITUTION:A solution to be measured introduced from an introducing opening of the solution to be measured 21 is passed through a urease immobilization enzyme 22 and urea in the solution to be measured is converted into NH3 or NH4<+>. A chemical reagent such as NaOH is added to this solution to be measured from a chemical reagent addition opening 27 and NH3, NH4<+> are turned into gaseous NH3. After that, the solution to be measured is entered to the second room 4 of a flow cell 1. On th other hand, an absorption solution 26 such as HCl is introduced into the first room 3 of the flow cell 1 and is reacted with NH3 permiting a gas permeable membrane 2. The reaction of the solution 26 with gaseous NH3 in the room 3 is detected as the variation of electric conductivity of the solution 26 by electrodes 7, 7'. The concentration of urea in the solution to be measured is determined from the variation quantity of this electric conductivity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55165597A JPS5788358A (en) | 1980-11-25 | 1980-11-25 | Method and apparatus for measuring urea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55165597A JPS5788358A (en) | 1980-11-25 | 1980-11-25 | Method and apparatus for measuring urea |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5788358A true JPS5788358A (en) | 1982-06-02 |
Family
ID=15815366
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55165597A Pending JPS5788358A (en) | 1980-11-25 | 1980-11-25 | Method and apparatus for measuring urea |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5788358A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60165551A (en) * | 1984-02-08 | 1985-08-28 | Yokogawa Hokushin Electric Corp | Method and device for measuring urea nitrogen using immobilized enzyme |
| WO2013173546A1 (en) * | 2012-05-16 | 2013-11-21 | Dionex Corporation | Surrogate addition device and method of analyte concentration |
| WO2019007588A1 (en) * | 2017-07-05 | 2019-01-10 | Anvajo GmbH | Device and method for detecting a specific analyte in a liquid sample and uses of said device |
-
1980
- 1980-11-25 JP JP55165597A patent/JPS5788358A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60165551A (en) * | 1984-02-08 | 1985-08-28 | Yokogawa Hokushin Electric Corp | Method and device for measuring urea nitrogen using immobilized enzyme |
| WO2013173546A1 (en) * | 2012-05-16 | 2013-11-21 | Dionex Corporation | Surrogate addition device and method of analyte concentration |
| CN104303038A (en) * | 2012-05-16 | 2015-01-21 | 戴安公司 | Surrogate addition device and method of analyte concentration |
| WO2019007588A1 (en) * | 2017-07-05 | 2019-01-10 | Anvajo GmbH | Device and method for detecting a specific analyte in a liquid sample and uses of said device |
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