[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

JP2011229474A - Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided with the same - Google Patents

Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided with the same Download PDF

Info

Publication number
JP2011229474A
JP2011229474A JP2010103766A JP2010103766A JP2011229474A JP 2011229474 A JP2011229474 A JP 2011229474A JP 2010103766 A JP2010103766 A JP 2010103766A JP 2010103766 A JP2010103766 A JP 2010103766A JP 2011229474 A JP2011229474 A JP 2011229474A
Authority
JP
Japan
Prior art keywords
incubator
observation
sliding contact
contact cover
microscope
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2010103766A
Other languages
Japanese (ja)
Inventor
Hideji Tsuchiya
秀治 土屋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tokai Hit Co Ltd
Original Assignee
Tokai Hit Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tokai Hit Co Ltd filed Critical Tokai Hit Co Ltd
Priority to JP2010103766A priority Critical patent/JP2011229474A/en
Priority to PCT/JP2011/001282 priority patent/WO2011135772A1/en
Publication of JP2011229474A publication Critical patent/JP2011229474A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PROBLEM TO BE SOLVED: To provide a contact cover used for an incubator for observation by a microscope, preventing the formation of spaces between the inner surface of a lens insertion hole and object lens, allowing precise management of temperature, humidity and the like of the environment in the space accommodating samples, and not requiring the production of a sliding contact cover with different dimensions of lens insertion hole diameter for each diameter dimension of object lenses, and to provide an incubator for observation by the microscope provided with the sliding contact cover.SOLUTION: A sheet 5 for the sliding contact cover 3 is formed from silicone rubber, which is a transparent material, and is flexible. The sheet 5 has a thin disk shape, and a circular lens insertion hole 7 is formed in the center thereof. The object lens T is inserted into the lens insertion hole 7. Since the sheet 5 is flexible, the diameter of the lens insertion hole 7 is expanded slightly by the lens barrel of the object lens T, and the inner surface of the lens insertion hole 7 adheres to the outer surface of the lens barrel of the object lens T. The state is such that no space is formed between the inner surface of the lens insertion hole 7 and the object lens T.

Description

本発明は顕微鏡のステージに載せた状態で細胞等の観察試料を培養しながら観察することができる顕微鏡観察用培養器に用いる摺接カバー、及び当該摺接カバーを備えた顕微鏡観察用培養器に関するものである。   The present invention relates to a sliding contact cover used in an incubator for microscope observation that can be observed while culturing an observation sample such as a cell on a stage of a microscope, and an incubator for microscope observation provided with the sliding contact cover. Is.

細胞等の観察試料を培養するためには、温度、湿度等の環境を管理する必要がある。顕微鏡のステージに載せた状態で温度、湿度等の環境を管理して細胞等の観察試料を培養し、この状態を観察することができる顕微鏡観察用培養器があり、この種の顕微鏡観察用培養器には特許文献１に記載されたものがある。
この顕微鏡観察用培養器は、観察試料が収容される試料収容空間を有する培養器本体を備えており、この培養器本体の上面（蓋体）には試料収容空間に連通する穴が形成されている。培養器本体の上面には、上記穴を覆う状態に顕微鏡観察用培養器に用いる摺接カバーが載置されている。この摺接カバーはガラス等の硬質材料から成り、摺接カバーには対物レンズが挿入されるレンズ挿入穴が形成されている。 In order to culture an observation sample such as a cell, it is necessary to manage the environment such as temperature and humidity. There is an incubator for microscope observation that can observe the state by cultivating observation samples such as cells by controlling the environment such as temperature and humidity while placed on the stage of the microscope. There is a vessel described in Patent Document 1.
This incubator for microscopic observation includes an incubator body having a sample storage space in which an observation sample is stored, and a hole communicating with the sample storage space is formed on the upper surface (lid body) of the incubator body. Yes. On the upper surface of the incubator main body, a sliding contact cover used for the incubator for microscope observation is placed so as to cover the hole. The sliding contact cover is made of a hard material such as glass, and the sliding contact cover has a lens insertion hole into which the objective lens is inserted.

また、顕微鏡観察用培養器は、ヒーター等から成る環境管理手段を備えており、この環境管理手段によって試料収容空間内の温度、湿度等の環境が所定の状態に保たれる。
この顕微鏡観察用培養器では、培養器本体を顕微鏡のステージに設置し、且つレンズ挿入穴に対物レンズを挿入した状態で顕微鏡観察を行う。また、ステージと共に培養器本体を水平方向へ動作させて観察位置を変更する。この動作の際には摺接カバーに対し培養器本体の上面が摺動する。 The incubator for microscope observation is provided with environmental management means including a heater and the like, and the environment such as temperature and humidity in the sample storage space is maintained in a predetermined state by the environmental management means.
In this incubator for microscope observation, the incubator body is set on the stage of the microscope, and the microscope is observed with the objective lens inserted in the lens insertion hole. Also, the observation position is changed by moving the incubator body in the horizontal direction together with the stage. In this operation, the upper surface of the incubator body slides with respect to the sliding contact cover.

特開２００４−１４１１４３号公報JP 2004-141143 A

しかしながら、従来の顕微鏡観察用培養器では、上記したように摺接カバーが硬質材料から成っているので、レンズ挿入穴を対物レンズの径寸法よりも一回り大きく形成する必要がある。このため、レンズ挿入穴の内周面と対物レンズとの間にはどうしても隙間ができてしまい、試料収容空間内の温度、湿度等の環境を高精度に管理することが難しい。
また、対物レンズは、倍率などの違いにより径寸法の異なるものが複数種類あるため、対物レンズの径寸法ごとにレンズ挿入穴の径寸法が異なる摺接カバーを製作する必要があり、摺接カバーの種類が多くなってしまう。 However, in the conventional incubator for microscope observation, since the sliding contact cover is made of a hard material as described above, it is necessary to form the lens insertion hole slightly larger than the diameter dimension of the objective lens. For this reason, a gap is inevitably formed between the inner peripheral surface of the lens insertion hole and the objective lens, and it is difficult to manage the environment such as temperature and humidity in the sample storage space with high accuracy.
In addition, since there are multiple types of objective lenses with different diameters due to differences in magnification, etc., it is necessary to manufacture a sliding contact cover in which the diameter dimension of the lens insertion hole is different for each objective lens diameter. There will be more types.

本発明は上記従来の問題点に着目して為されたものであり、レンズ挿入穴の内周面と対物レンズとの間に隙間ができるのを防止でき、試料収容空間内の温度、湿度等の環境を高精度に管理することが可能となり、対物レンズの径寸法ごとにレンズ挿入穴の径寸法が異なる摺接カバーを製作する必要のない顕微鏡観察用培養器に用いる摺接カバー、当該摺接カバーを備えた顕微鏡観察用培養器を提供することを、その目的とする。   The present invention has been made paying attention to the above-mentioned conventional problems, and can prevent a gap from being formed between the inner peripheral surface of the lens insertion hole and the objective lens, and the temperature, humidity, etc. in the sample accommodation space. Can be managed with high accuracy, and there is no need to produce a sliding cover in which the diameter of the lens insertion hole differs for each diameter of the objective lens. It is an object of the present invention to provide a microscopic observation incubator provided with a contact cover.

本発明は上記課題を解決するためになされたものであり、請求項１の発明は、観察試料が収容される試料収容空間を有し、且つ前記試料収容空間に連通する穴が上面に形成された培養器本体と、前記試料収容空間内の温度、湿度等の環境を所定の状態に管理する環境管理手段とを有する顕微鏡観察用培養器に用いられ、顕微鏡のステージに設置した前記培養器本体の上面に前記穴を覆う状態に載置される摺接カバーであって、前記摺接カバーは顕微鏡の対物レンズが挿入されるレンズ挿入穴を有し、前記レンズ挿入穴に対物レンズを挿入した状態で、前記培養器本体を水平方向へ動作させると、前記培養器本体の上面に摺接する顕微鏡観察用培養器に用いる摺接カバーにおいて、
前記摺接カバーは、前記培養器本体の上面に摺接するフレームと、前記フレームに取り付けられ柔軟性を有し、且つ前記レンズ挿入穴が形成されたシートとによって構成されることを特徴とする顕微鏡観察用培養器に用いる摺接カバーである。 The present invention has been made to solve the above problems, and the invention of claim 1 has a sample storage space in which an observation sample is stored, and a hole communicating with the sample storage space is formed on the upper surface. The incubator body used in a microscope observation incubator having an incubator body and environmental management means for managing an environment such as temperature and humidity in the sample storage space in a predetermined state. The sliding contact cover is placed on the upper surface of the lens so as to cover the hole, and the sliding contact cover has a lens insertion hole into which an objective lens of a microscope is inserted, and the objective lens is inserted into the lens insertion hole. In the state, when the incubator body is operated in the horizontal direction, in the sliding contact cover used for the incubator for microscope observation that is in sliding contact with the upper surface of the incubator body,
The sliding contact cover is constituted by a frame that is in sliding contact with the upper surface of the incubator body, and a sheet that is attached to the frame and has flexibility and has the lens insertion hole formed therein. It is a sliding contact cover used for an incubator for observation.

請求項２の発明は、請求項１に記載した顕微鏡観察用培養器に用いる摺接カバーにおいて、
シートの下面は、培養器本体の上面に摺接するフレームの下面よりも上方に位置していることを特徴とする顕微鏡観察用培養器に用いる摺接カバーである。 Invention of Claim 2 is the sliding contact cover used for the incubator for microscope observation described in Claim 1,
The lower surface of the sheet is a sliding contact cover used for an incubator for microscope observation, which is located above the lower surface of the frame that is in sliding contact with the upper surface of the incubator body.

請求項３の発明は、請求項１また２に記載した顕微鏡観察用培養器に用いる摺接カバーにおいて、
シートは透明材料によって構成されていることを特徴とする顕微鏡観察用培養器に用いる摺接カバーである。 Invention of Claim 3 is the sliding contact cover used for the incubator for microscope observation described in Claim 1 or 2,
The sheet is a sliding contact cover used in a microscopic observation incubator characterized by being made of a transparent material.

請求項４の発明は、請求項１から３のいずれかに記載した顕微鏡観察用培養器に用いる摺接カバーにおいて、
シートはシリコーンゴムによって構成されていることを特徴とする顕微鏡観察用培養器に用いる摺接カバーである。 Invention of Claim 4 is the sliding contact cover used for the incubator for microscope observation in any one of Claim 1 to 3,
The sheet is a sliding contact cover used in a microscopic observation incubator characterized by being made of silicone rubber.

請求項５の発明は、観察試料が収容される試料収容空間を有し、且つ前記試料収容空間に連通する穴が上面に形成された培養器本体と、前記試料収容空間内の温度、湿度等の環境を所定の状態に管理する環境管理手段とを有し、顕微鏡のステージに前記培養器本体を設置して、前記培養器本体を水平方向へ動作させて観察試料の観察位置を変更する顕微鏡観察用培養器において、
請求項１から４のいずれかの顕微鏡観察用培養器に用いる摺接カバーを備えてことを特徴とする顕微鏡観察用培養器である。 The invention of claim 5 includes a culture vessel main body having a sample storage space in which an observation sample is stored and a hole communicating with the sample storage space is formed on the upper surface, and the temperature, humidity, etc. in the sample storage space. A microscope that changes the observation position of the observation sample by installing the incubator body on a microscope stage and operating the incubator body in a horizontal direction. In the incubator for observation,
A microscopic observation incubator comprising a sliding contact cover used in the microscopic observation incubator according to any one of claims 1 to 4.

本発明の顕微鏡観察用培養器に用いる摺接カバー、当該摺接カバーを備えた顕微鏡観察用培養器によれば、レンズ挿入穴の内周面が対物レンズに密着するので、レンズ挿入穴の内周面と対物レンズとの間に隙間ができるのを防止できる。さらに、レンズ挿入穴の内周面と対物レンズとの間に隙間がないので、試料収容空間内の温度、湿度等の環境を高精度に管理することが可能となる。
また、レンズ挿入穴は、ある程度の径寸法まで拡径可能となっているので、一つの摺接カバーで径寸法の異なる複数種類の対物レンズに対応することができる。従って、対物レンズの径寸法ごとにレンズ挿入穴の径寸法が異なる摺接カバーを製作する必要がなくなる。 According to the sliding contact cover used in the microscope observation incubator of the present invention and the microscope observation incubator provided with the sliding contact cover, the inner peripheral surface of the lens insertion hole is in close contact with the objective lens. It is possible to prevent a gap from being formed between the peripheral surface and the objective lens. Furthermore, since there is no gap between the inner peripheral surface of the lens insertion hole and the objective lens, the environment such as temperature and humidity in the sample storage space can be managed with high accuracy.
Moreover, since the lens insertion hole can be expanded to a certain diameter, a single sliding cover can accommodate a plurality of types of objective lenses having different diameters. Therefore, it is not necessary to manufacture a sliding contact cover in which the diameter dimension of the lens insertion hole is different for each diameter dimension of the objective lens.

本発明の実施の形態に係る顕微鏡観察用培養器の斜視図である。It is a perspective view of the incubator for microscope observation which concerns on embodiment of this invention. 図１の顕微鏡観察用培養器の摺接カバーの斜視図である。It is a perspective view of the sliding contact cover of the incubator for microscope observation of FIG. 図１の顕微鏡観察用培養器の側断面図である。It is a sectional side view of the incubator for microscope observation of FIG. 培養器本体を上下方向へ動作させる様子を示す図である。It is a figure which shows a mode that an incubator main body is operated to an up-down direction. 対物レンズを上下方向へ動作させる様子を示す図である。It is a figure which shows a mode that an objective lens is operated to an up-down direction.

本発明の実施の形態に係る顕微鏡観察用培養器１を図面にしたがって説明する。
顕微鏡観察用培養器１は摺接カバー３、培養器本体１５等によって構成されている。
摺接カバー３の詳細な構成について図２にしたがって説明する。
符号５はシートを示し、このシート５は透明材料であるシリコーンゴムから成っており、シート５は柔軟性を有している。このシート５は薄い円盤状を為しており、シート５の中央には、円形のレンズ挿入穴７が形成されている。 A microscopic observation incubator 1 according to an embodiment of the present invention will be described with reference to the drawings.
The microscopic observation incubator 1 includes a sliding contact cover 3, an incubator main body 15, and the like.
A detailed configuration of the sliding contact cover 3 will be described with reference to FIG.
Reference numeral 5 denotes a sheet, which is made of a silicone rubber that is a transparent material, and the sheet 5 has flexibility. The sheet 5 has a thin disk shape, and a circular lens insertion hole 7 is formed at the center of the sheet 5.

符号９はリング状のフレームを示し、このフレーム９は、比較的摩擦抵抗の小さいプラスチックから成る上側板１１と下側板１３とから成る。この上側板１１と下側板１３とによってシート５の外周縁は挟まれた状態で保持されており、シート５の下面はフレーム９の下面よりも上方に位置している（図３参照）。   Reference numeral 9 denotes a ring-shaped frame, and the frame 9 is composed of an upper plate 11 and a lower plate 13 made of a plastic having a relatively small frictional resistance. The outer peripheral edge of the sheet 5 is held between the upper plate 11 and the lower plate 13, and the lower surface of the sheet 5 is located above the lower surface of the frame 9 (see FIG. 3).

培養器本体１５の詳細な構成について図１、図３にしたがって説明する。
培養器本体１５はベース部１７と蓋体２３によって構成されている。
ベース部１７は、ある程度の厚さ寸法を有する略枠状を為しており、上面と下面に開口を有している。また、ベース部１７の内周面には水槽１９が設けられている。このベース部１７の内部が試料収容空間２１となっている。 A detailed configuration of the incubator body 15 will be described with reference to FIGS.
The incubator main body 15 includes a base portion 17 and a lid body 23.
The base portion 17 has a substantially frame shape having a certain thickness, and has openings on the upper surface and the lower surface. A water tank 19 is provided on the inner peripheral surface of the base portion 17. The inside of the base portion 17 is a sample storage space 21.

蓋体２３は、開口２７を有する蓋板部２５と、開口２７に嵌められた透明ガラスヒーター２９によって構成されている。透明ガラスヒーター２９の中心には丸穴３１が形成されており、この丸穴３１は摺接カバー３の外径よりも小さく、且つレンズ挿入穴７よりも大きい径寸法に設定されている。
試料収容空間２１には図示しないガス供給装置によってＣＯ２ガス等が供給されるようになっており、また、水槽１９には図示しない給水装置によって水が供給されるようになっている。 The lid body 23 includes a lid plate portion 25 having an opening 27 and a transparent glass heater 29 fitted in the opening 27. A round hole 31 is formed at the center of the transparent glass heater 29, and the round hole 31 is set to have a diameter smaller than the outer diameter of the sliding contact cover 3 and larger than the lens insertion hole 7.
CO 2 gas or the like is supplied to the sample storage space 21 by a gas supply device (not shown), and water is supplied to the water tank 19 by a water supply device (not shown).

符号３３はヒータープレートを示し、このヒータープレート３３は枠体３５と、この枠体３５に嵌められた透明ガラスヒーター３７によって構成されている。
環境管理手段は水槽１９、透明ガラスヒーター２９、図示しないガス供給装置、図示しない給水装置及び透明ガラスヒーター３７によって構成されている。 Reference numeral 33 denotes a heater plate, and the heater plate 33 includes a frame body 35 and a transparent glass heater 37 fitted to the frame body 35.
The environmental management means includes a water tank 19, a transparent glass heater 29, a gas supply device (not shown), a water supply device (not shown), and a transparent glass heater 37.

図１において符号３９は顕微鏡のステージを示し、このステージ３９は、上下に重ねて配置された二枚の駆動板４１、４３を備えている。上側の駆動板４１は左右方向（ａ−ｂ方向）へ水平駆動し、下側の駆動板４３は上側の駆動板４１と共に前後方向（ｃ−ｄ方向）へ水平駆動する。また、駆動板４１、４３は上下方向（ｅ−ｆ方向）へ駆動するようになっている。駆動板４１、４３には開口４５が形成されている（図３参照）。   In FIG. 1, reference numeral 39 denotes a microscope stage, and this stage 39 includes two drive plates 41 and 43 arranged one above the other. The upper drive plate 41 is horizontally driven in the left-right direction (ab direction), and the lower drive plate 43 is horizontally driven in the front-rear direction (cd direction) together with the upper drive plate 41. The drive plates 41 and 43 are driven in the vertical direction (ef direction). Openings 45 are formed in the drive plates 41 and 43 (see FIG. 3).

次に、顕微鏡観察用培養器１の使用方法について説明する。
図３に示すようにヒータープレート３３を駆動板４１の開口４５に嵌めて、開口４５を塞ぐ。そして、ヒータープレート３３上に試料収容空間２１が位置するようにベース部１７を駆動板４１に載せる。次に、細胞等の観察試料Ｓを培養液と共にディッシュＤに入れ、このディッシュＤを試料収容空間２１に収容する。次いで、蓋体２３をベース部１７に載置してベース部１７の上面開口を覆う状態とする。この状態では、蓋体２３の丸穴３１は試料収容空間２１に連通している。
摺接カバー３を蓋体２３の透明ガラスヒーター２９に載置して、この摺接カバー３によって丸穴３１を覆い、レンズ挿入穴７を丸穴３１に対応させる。
前述したように摺接カバー３のシート５は透明材料から成っているので、試料収容空間２１に収容された観察試料Ｓを外部から視認可能になっている。 Next, a method for using the microscopic observation incubator 1 will be described.
As shown in FIG. 3, the heater plate 33 is fitted into the opening 45 of the drive plate 41 to close the opening 45. Then, the base portion 17 is placed on the drive plate 41 so that the sample accommodation space 21 is positioned on the heater plate 33. Next, the observation sample S such as cells is placed in the dish D together with the culture solution, and the dish D is stored in the sample storage space 21. Next, the lid body 23 is placed on the base portion 17 to cover the upper surface opening of the base portion 17. In this state, the round hole 31 of the lid body 23 communicates with the sample storage space 21.
The sliding contact cover 3 is placed on the transparent glass heater 29 of the lid 23, the round hole 31 is covered with the sliding contact cover 3, and the lens insertion hole 7 is made to correspond to the round hole 31.
As described above, since the sheet 5 of the sliding contact cover 3 is made of a transparent material, the observation sample S accommodated in the sample accommodation space 21 can be visually recognized from the outside.

次に、対物レンズＴをレンズ挿入穴７に挿入する。前述したようにシート５は柔軟性を有しているので、レンズ挿入穴７は対物レンズＴの鏡筒によって僅かに拡径され、レンズ挿入穴７の内周面が対物レンズＴの鏡筒の外周面に密着する。従って、レンズ挿入穴７の内周面と対物レンズＴとの間には隙間がない状態となる。
また、レンズ挿入穴７に挿入した対物レンズＴは、丸穴３１から試料収容空間２１へ入り込んだ状態となっている。
試料収容空間２１はベース部１７、蓋体２３、摺接カバー３、対物レンズＴ、ヒータープレート３３及び駆動板４１によって画成された空間となる。 Next, the objective lens T is inserted into the lens insertion hole 7. As described above, since the sheet 5 has flexibility, the lens insertion hole 7 is slightly enlarged in diameter by the lens barrel of the objective lens T, and the inner peripheral surface of the lens insertion hole 7 is the lens barrel of the objective lens T. Close contact with the outer peripheral surface. Therefore, there is no gap between the inner peripheral surface of the lens insertion hole 7 and the objective lens T.
Further, the objective lens T inserted into the lens insertion hole 7 is in a state of entering the sample accommodation space 21 from the round hole 31.
The sample storage space 21 is a space defined by the base portion 17, the lid body 23, the sliding contact cover 3, the objective lens T, the heater plate 33, and the drive plate 41.

次いで、透明ガラスヒーター２９、３７、図示しないガス供給装置及び図示しない給水装置によって、試料収容空間２１内の温度、湿度等の環境を所定の状態に管理する。上記したようにレンズ挿入穴７の内周面と対物レンズＴとの間には隙間がないので、試料収容空間２１を外部と略完全に隔絶でき、試料収容空間２１内の温度、湿度等の環境を高精度に管理することが可能である。   Next, the environment such as temperature and humidity in the sample storage space 21 is managed in a predetermined state by the transparent glass heaters 29 and 37, a gas supply device (not shown), and a water supply device (not shown). As described above, since there is no gap between the inner peripheral surface of the lens insertion hole 7 and the objective lens T, the sample storage space 21 can be substantially completely isolated from the outside, and the temperature, humidity, etc. in the sample storage space 21 can be determined. It is possible to manage the environment with high accuracy.

観察試料Ｓの観察位置を変更する場合には、ステージ３９の駆動板４１、４３と共に培養器本体１５を水平方向へ動作させる。前述したようにシート５の下面はフレーム９の下面よりも上方に位置しているので、シート５は透明ガラスヒーター２９に摺接せず、フレーム９だけが透明ガラスヒーター２９に摺接する。従って、透明ガラスヒーター２９の上面、即ち培養器本体１５の上面はフレーム９の下面に対しスムーズに摺動する。   When changing the observation position of the observation sample S, the incubator body 15 is moved in the horizontal direction together with the drive plates 41 and 43 of the stage 39. As described above, since the lower surface of the sheet 5 is located above the lower surface of the frame 9, the sheet 5 does not slide on the transparent glass heater 29, and only the frame 9 slides on the transparent glass heater 29. Therefore, the upper surface of the transparent glass heater 29, that is, the upper surface of the incubator body 15 slides smoothly with respect to the lower surface of the frame 9.

対物レンズＴの焦点を合わせるため、ステージ３９の駆動板４１、４３と共に培養器本体１５を上下方向へ動作させる。前述したようにシート５は柔軟性を有しているので、図４に示すようにシート５は培養器本体１５の上下方向の動作に応じて伸縮する。従って、レンズ挿入穴７の内周面は対物レンズＴの鏡筒に対し密着した状態を保つことができる。よって、レンズ挿入穴７の内周面と対物レンズＴとの間に隙間ができるのを確実に防止できる。
また、図５に示すように対物レンズＴを上下方向へ動作させる場合でも、上記と同様にシート５は対物レンズＴの上下方向の動作に応じて伸縮する。従って、レンズ挿入穴７の内周面は対物レンズＴの鏡筒に対し密着した状態を保つことができ、レンズ挿入穴７の内周面と対物レンズＴとの間に隙間ができるのを確実に防ぐことが可能である。 In order to focus the objective lens T, the incubator body 15 is moved in the vertical direction together with the drive plates 41 and 43 of the stage 39. Since the sheet 5 has flexibility as described above, the sheet 5 expands and contracts according to the vertical movement of the incubator body 15 as shown in FIG. Therefore, the inner peripheral surface of the lens insertion hole 7 can be kept in close contact with the lens barrel of the objective lens T. Therefore, it is possible to reliably prevent a gap from being formed between the inner peripheral surface of the lens insertion hole 7 and the objective lens T.
Further, as shown in FIG. 5, even when the objective lens T is moved in the vertical direction, the sheet 5 expands and contracts according to the vertical movement of the objective lens T as described above. Accordingly, the inner peripheral surface of the lens insertion hole 7 can be kept in close contact with the lens barrel of the objective lens T, and it is ensured that a gap is formed between the inner peripheral surface of the lens insertion hole 7 and the objective lens T. It is possible to prevent.

また、シート５は柔軟性を有しており、このシート５のレンズ挿入穴７は、ある程度の径寸法まで拡径可能となっているので、一つの摺接カバー３で径寸法の異なる複数種類の対物レンズＴに対応することができる。従って、対物レンズＴの径寸法ごとにレンズ挿入穴７の径寸法が異なる摺接カバー３を製作する必要がなくなる。   Further, since the sheet 5 has flexibility and the lens insertion hole 7 of the sheet 5 can be expanded to a certain diameter, a plurality of types having different diameters by one sliding contact cover 3 are provided. It can correspond to the objective lens T. Therefore, it is not necessary to manufacture the sliding cover 3 in which the diameter dimension of the lens insertion hole 7 is different for each diameter dimension of the objective lens T.

以上、本発明の実施の形態について詳述してきたが、具体的構成は、この実施の形態に限られるものではなく、本発明の要旨を逸脱しない範囲における設計の変更などがあっても発明に含まれる。
上記実施の形態では、シート５をシリコーンゴムによって構成したが、シート５をウレタンゴム等によって構成してもよい。
また、フレーム９をリング状に形成したが、フレームの形状はリング状に限定されず、例えば、フレーム９を矩形状に形成してもよい。
さらに、フレーム９をプラスチックによって構成したが、フレーム９を木材やガラス等によって構成してもよい。 The embodiment of the present invention has been described in detail above. However, the specific configuration is not limited to this embodiment, and the present invention can be changed even if there is a design change without departing from the gist of the present invention. included.
In the above embodiment, the sheet 5 is made of silicone rubber, but the sheet 5 may be made of urethane rubber or the like.
Further, although the frame 9 is formed in a ring shape, the shape of the frame is not limited to the ring shape, and for example, the frame 9 may be formed in a rectangular shape.
Furthermore, although the frame 9 is made of plastic, the frame 9 may be made of wood or glass.

本発明は、細胞等の観察試料を培養しながら観察することができる顕微鏡観察用培養器の製造業に利用可能である。   INDUSTRIAL APPLICABILITY The present invention can be used in the manufacturing industry of a microscopic observation incubator that can observe observation samples such as cells while culturing them.

１…顕微鏡観察用培養器 ３…摺接カバー ５…シート
７…レンズ挿入穴 ９…フレーム １１…上側板
１３…下側板 １５…培養器本体 １７…ベース部
１９…水槽 ２１…試料収容空間 ２３…蓋体
２５…蓋板部 ２７…開口 ２９…透明ガラスヒーター
３１…丸穴 ３３…ヒータープレート ３５…枠体
３７…透明ガラスヒーター ３９…ステージ ４１、４３…駆動板
４５…開口
Ｓ…観察試料 Ｄ…ディッシュ Ｔ…対物レンズ DESCRIPTION OF SYMBOLS 1 ... Microscope observation incubator 3 ... Sliding contact cover 5 ... Sheet 7 ... Lens insertion hole 9 ... Frame 11 ... Upper side plate 13 ... Lower side plate 15 ... Incubator main body 17 ... Base part 19 ... Water tank 21 ... Sample accommodation space 23 ... Lid 25 ... Lid plate part 27 ... Opening 29 ... Transparent glass heater 31 ... Round hole 33 ... Heater plate 35 ... Frame 37 ... Transparent glass heater 39 ... Stages 41, 43 ... Drive plate 45 ... Opening S ... Observation sample D ... Dish T ... Objective lens

Claims (5)

観察試料が収容される試料収容空間を有し、且つ前記試料収容空間に連通する穴が上面に形成された培養器本体と、前記試料収容空間内の温度、湿度等の環境を所定の状態に管理する環境管理手段とを有する顕微鏡観察用培養器に用いられ、顕微鏡のステージに設置した前記培養器本体の上面に前記穴を覆う状態に載置される摺接カバーであって、前記摺接カバーは顕微鏡の対物レンズが挿入されるレンズ挿入穴を有し、前記レンズ挿入穴に対物レンズを挿入した状態で、観察試料の観察位置を変更するため前記培養器本体を水平方向へ動作させると、前記培養器本体の上面に摺接する顕微鏡観察用培養器に用いる摺接カバーにおいて、
前記摺接カバーは、前記培養器本体の上面に摺接するフレームと、前記フレームに取り付けられ柔軟性を有し、且つ前記レンズ挿入穴が形成されたシートとによって構成されることを特徴とする顕微鏡観察用培養器に用いる摺接カバー。 An incubator body having a sample storage space in which an observation sample is stored and a hole communicating with the sample storage space is formed on the upper surface, and an environment such as temperature and humidity in the sample storage space in a predetermined state A sliding contact cover that is used in a microscope observation incubator having an environmental management means for managing, and is placed in a state of covering the hole on the upper surface of the incubator body installed on a microscope stage, The cover has a lens insertion hole into which the objective lens of the microscope is inserted, and the incubator body is moved in the horizontal direction in order to change the observation position of the observation sample with the objective lens inserted in the lens insertion hole. In the sliding contact cover used for the incubator for microscopic observation that is in sliding contact with the upper surface of the incubator body
The sliding contact cover is constituted by a frame that is in sliding contact with the upper surface of the incubator body, and a sheet that is attached to the frame and has flexibility and has the lens insertion hole formed therein. Sliding contact cover used for incubator for observation. 請求項１に記載した顕微鏡観察用培養器に用いる摺接カバーにおいて、
シートの下面は、培養器本体の上面に摺接するフレームの下面よりも上方に位置していることを特徴とする顕微鏡観察用培養器に用いる摺接カバー。 In the sliding cover used for the incubator for microscopic observation according to claim 1,
A sliding contact cover used for an incubator for microscope observation, wherein the lower surface of the sheet is positioned above the lower surface of the frame that is in sliding contact with the upper surface of the incubator body. 請求項１また２に記載した顕微鏡観察用培養器に用いる摺接カバーにおいて、
シートは透明材料によって構成されていることを特徴とする顕微鏡観察用培養器に用いる摺接カバー。 In the sliding contact cover used for the incubator for microscope observation according to claim 1 or 2,
A sliding contact cover used for an incubator for microscope observation, characterized in that the sheet is made of a transparent material. 請求項１から３のいずれかに記載した顕微鏡観察用培養器に用いる摺接カバーにおいて、
シートはシリコーンゴムによって構成されていることを特徴とする顕微鏡観察用培養器に用いる摺接カバー。 In the sliding contact cover used in the incubator for microscope observation according to any one of claims 1 to 3,
A sliding contact cover used for a microscopic observation incubator, wherein the sheet is made of silicone rubber. 観察試料が収容される試料収容空間を有し、且つ前記試料収容空間に連通する穴が上面に形成された培養器本体と、前記試料収容空間内の温度、湿度等の環境を所定の状態に管理する環境管理手段とを有し、顕微鏡のステージに前記培養器本体を設置して、前記培養器本体を水平方向へ動作させて観察試料の観察位置を変更する顕微鏡観察用培養器において、
請求項１から４のいずれかの顕微鏡観察用培養器に用いる摺接カバーを備えてことを特徴とする顕微鏡観察用培養器。 An incubator body having a sample storage space in which an observation sample is stored and a hole communicating with the sample storage space is formed on the upper surface, and an environment such as temperature and humidity in the sample storage space in a predetermined state In an incubator for microscope observation having an environment management means for managing, installing the incubator body on a stage of a microscope, and operating the incubator body in a horizontal direction to change an observation position of an observation sample.
5. A microscopic observation incubator comprising a sliding contact cover used in the microscopic observation incubator according to claim 1.
JP2010103766A 2010-04-28 2010-04-28 Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided with the same Pending JP2011229474A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2010103766A JP2011229474A (en) 2010-04-28 2010-04-28 Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided with the same
PCT/JP2011/001282 WO2011135772A1 (en) 2010-04-28 2011-03-04 Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided therewith

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2010103766A JP2011229474A (en) 2010-04-28 2010-04-28 Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided with the same

Publications (1)

Publication Number Publication Date
JP2011229474A true JP2011229474A (en) 2011-11-17

Family

ID=44861101

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010103766A Pending JP2011229474A (en) 2010-04-28 2010-04-28 Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided with the same

Country Status (2)

Country Link
JP (1) JP2011229474A (en)
WO (1) WO2011135772A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015079220A (en) * 2013-10-18 2015-04-23 株式会社ニコン Cover member, microscope, observation method and observation instrument
EP3175279A1 (en) * 2014-07-29 2017-06-07 Leica Mikrosysteme GmbH Light microscope having a sample stage for cryomicroscopy

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104178420B (en) * 2014-09-05 2016-04-13 中国科学技术大学 A kind of microscope viable cell culture environment Controlling System
CN104730048B (en) * 2015-01-07 2017-07-07 鲁东大学 A kind of copper-base graphite alkene objective table mechanical control system for carrying fluorescent samples
CN106434342A (en) * 2016-09-24 2017-02-22 成都测迪森生物科技有限公司 Cell culture dish

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4562165B2 (en) * 2002-08-28 2010-10-13 株式会社東海ヒット Microscope incubator
JP4669302B2 (en) * 2005-02-24 2011-04-13 富士通株式会社 Lens cover

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015079220A (en) * 2013-10-18 2015-04-23 株式会社ニコン Cover member, microscope, observation method and observation instrument
EP3175279A1 (en) * 2014-07-29 2017-06-07 Leica Mikrosysteme GmbH Light microscope having a sample stage for cryomicroscopy
JP2017522607A (en) * 2014-07-29 2017-08-10 ライカ ミクロジュステーメ ゲーエムベーハー Optical microscope with sample stage for cryomicroscopy
US10901196B2 (en) 2014-07-29 2021-01-26 Leica Mikrosysteme Gmbh Light microscope having a sample stage for cryomicroscopy
EP3175279B1 (en) * 2014-07-29 2023-02-15 Leica Mikrosysteme GmbH Light microscope having a sample stage for cryomicroscopy

Also Published As

Publication number Publication date
WO2011135772A1 (en) 2011-11-03

Similar Documents

Publication Publication Date Title
WO2011135772A1 (en) Sliding contact cover used for incubator for observation by microscope and incubator for observation by microscope provided therewith
JP4354446B2 (en) Microscope stage and microscope observation unit
EP2690167B1 (en) Culture apparatus for microscopic viewing and method of use thereof
EP1672405B1 (en) Observation apparatus
KR102230694B1 (en) Culture container
KR20090020614A (en) Culture monitoring system
Westerwalbesloh et al. A microfluidic photobioreactor for simultaneous observation and cultivation of single microalgal cells or cell aggregates
CN105378051A (en) Structure for culturing cells
WO2012095678A1 (en) Imaging chamber for supporting multiple investigation of cells and tissues by various techniques
WO2006005810A1 (en) An apparatus for imaging cells
JP5537855B2 (en) Culture observation system
JP5700954B2 (en) Culture vessel
KR20140049844A (en) Cell chip and container for cell chip
KR101816535B1 (en) Bio chip structure for comparative experiment
JP4578845B2 (en) Culture dish assembly
JP6183289B2 (en) Cell culture devices
JP6201647B2 (en) Microscope observation culture equipment
JP6641961B2 (en) Cell container mounting unit and mounting method using the same
JP2018200458A (en) Container for microscopic observation
JP6348073B2 (en) Culture dish storage container
US20130084633A1 (en) Petri dishes
US10606058B2 (en) Microscope slide for liquid cultures
JP2016208881A (en) Cell container
JP2007049968A (en) Tool for storing culture solution and lid of laboratory dish
US20100097695A1 (en) Incubation container system