CN202430229U - High-volume roller bottle for tissue culture - Google Patents
High-volume roller bottle for tissue culture Download PDFInfo
- Publication number
- CN202430229U CN202430229U CN 201120540766 CN201120540766U CN202430229U CN 202430229 U CN202430229 U CN 202430229U CN 201120540766 CN201120540766 CN 201120540766 CN 201120540766 U CN201120540766 U CN 201120540766U CN 202430229 U CN202430229 U CN 202430229U
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- feed
- opening
- bottle
- pipeline
- rolling bottle
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
- C12M27/12—Roller bottles; Roller tubes
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
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- Sustainable Development (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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Abstract
The utility model discloses a high-volume roller bottle for tissue culture. The roller bottle comprises a bottle body, a main bottle opening, a feed inlet and a discharge outlet, wherein the main bottle opening is arranged in the center of the top of the bottle body, the feed inlet is disposed on a side of the main bottle opening, the discharge outlet is arranged on a side of the main bottle opening, the discharge outlet and the feed inlet are symmetrically arranged around the main bottle opening, the feed inlet is sealed by a feed inlet pipeline connecting screw cap, the feed inlet pipeline connecting screw cap consists of a feed pipeline and an air inlet dewatering pipeline, the feed pipeline penetrates through a screw cap, the discharge outlet is sealed by a discharge outlet pipeline connecting screw cap, the discharge outlet pipeline connecting screw cap consists of a discharge pipeline and an air outlet dewatering pipeline, and the discharge pipeline penetrates through the screw cap. A magnetic stirring system with a complicated internal structure is omitted, the high-volume roller bottle for tissue culture is operated conveniently and is simple in structure, rotation speed is stable owing to a rotary shaking table, cells can be suspended sufficiently, dissolve of oxygen and growth of the cells are guaranteed, and accordingly the purpose of large-scale tissue culture is achieved.
Description
Technical field
The utility model relates to the special-purpose rolling bottle of a kind of cell cultures, is specifically related to a kind of at large-scale CO
2The large vol cell cultures rolling bottle that incubator uses.
Background technology
In the biological medicine industry, increasing cell amplification and the cell cultures of being used to of cell rolling bottle.Using more is 8 liters of glass rolling bottles (PIN 4500-8L) of the production of Dow corning (Corning) company; It has the rolling bottle inner cell liquid dissolved oxygen of raising and blended side guard; Through having the stirrer of plastics canvas-type impeller; Guarantee good stirring, thereby satisfy the demand of cell growth dissolved oxygen.But also there is certain problem in itself, mainly is: when the enchylema loading amount was bigger, mixed effect was not good; Because of magnetic agitation has the gap about 2cm apart from the bottle end, and stir shaft is longer, and mixing speed is unstable, can't make cell suspension grow; Stir shaft is installed more loaded down with trivial details, and fractures easily in bearing.So, in mass cell is cultivated, there is certain risk, need a kind of good mixing effect of exploitation, stabilization of speed, the large vol cell cultures rolling bottle that cell suspension is grown.
The utility model content
The purpose of the utility model provides a kind of large vol cell cultures rolling bottle, to improve the dissolved oxygen and the mixed effect of enchylema, increase the enchylema volume, and this cultivation rolling bottle has stable mixing speed.
In order to achieve the above object, the utility model provides a kind of large vol cell cultures rolling bottle, and this rolling bottle comprises:
Body;
Be arranged on the main bottleneck of body center of top;
Be arranged on the opening for feed of main bottleneck sidepiece; And,
Be arranged on the discharge port of main bottleneck sidepiece, this discharge port and opening for feed are the centrosymmetry setting with main bottleneck;
Wherein, described opening for feed is through the sealing of opening for feed pipe connection spiral cover, and this opening for feed pipe connection spiral cover comprises feed pipe and the inlet mouth drain water piping that runs through spiral cover; Described discharge port is through the sealing of discharge port pipe connection spiral cover, and this discharge port pipe connection spiral cover comprises discharge pipe and the air outlet drain water piping that runs through spiral cover.
Above-mentioned large vol cell cultures rolling bottle, wherein, the aspect ratio of described body is 0.9 ~ 1:1, is preferably 0.9:1.
Above-mentioned large vol cell cultures rolling bottle, wherein, the bore of described main bottleneck is greater than the bore of opening for feed and discharge port.
Above-mentioned large vol cell cultures rolling bottle, wherein, described main bottleneck is the frosted bottleneck, seals through bottle stopper.
Above-mentioned large vol cell cultures rolling bottle, wherein, described feed pipe, inlet mouth drain water piping, discharge pipe and air outlet drain water piping are silicone tube.
The glass cell rolling bottle that the utility model provides, volume reaches 15L, and bottle adopts peroxy-borate glass, has good intensity and stability, and for improving dissolved oxygen, body is selected suitable aspect ratio rate 0.9:1.This rolling bottle is designed with center bottleneck master bottleneck and two neck side direction bottlenecks (opening for feed and discharge port) that diameter is 45mm that diameter is 100mm, makes things convenient for the cleaning and the aseptic turnover material of cell rolling bottle.The cell cultures rolling bottle of the utility model has been cancelled the complicated magnetic agitation system of internal structure, and handled easily gos deep into the input and output material silicone tube of bottle, plays baffle effect.
Adopt novel large vol cell cultures rolling bottle, the enchylema volume of single rolling bottle can reach 7L, and uses rotary shaker; Abandon magnetic agitation, stablized the mixing speed of cell rolling bottle, thereby improved dissolved oxygen; Reach the good mixing effect, guarantee cell rolling bottle inner cell liquid homergy.
In sum, a kind of large vol cell cultures rolling bottle that the utility model provides, simple in structure, design ingenious; Install simply, easy to operate, through rotary shaking table, stabilization of speed; Can fully cell suspension be got up, guarantee cell dissolved oxygen and growth, thereby reach the purpose that mass cell is cultivated.
Description of drawings
Fig. 1 is the structural representation of a kind of large vol cell cultures rolling bottle of the utility model.
Embodiment
Specify the utility model preferred embodiment below in conjunction with accompanying drawing.
As shown in Figure 1, the large vol cell cultures rolling bottle of the utility model comprises: body 11, and body is a right cylinder, is arranged on the main bottleneck 12 of body center of top; Be arranged on the opening for feed 13 and discharge port 14 of main bottleneck 12 sidepieces; Wherein, this discharge port 14 is the centrosymmetry setting with opening for feed 13 with main bottleneck 12.
Described main bottleneck 12 is as Butterworth Hatch, and its diameter is greater than the diameter of opening for feed 13 and discharge port 14, and in other embodiment of the utility model, this main bottleneck is the frosted bottleneck, seals through bottle stopper.
Described opening for feed 13 is through the sealing of opening for feed pipe connection spiral cover (not shown), and this opening for feed pipe connection spiral cover comprises feed pipe and the inlet mouth drain water piping that runs through spiral cover; Described discharge port 14 is through the sealing of discharge port pipe connection spiral cover (not shown), and this discharge port pipe connection spiral cover comprises discharge pipe and the air outlet drain water piping that runs through spiral cover; Above-mentioned feed pipe, inlet mouth drain water piping, discharge pipe and air outlet drain water piping are silicone tube.
In some preferred embodiments of the utility model, described rolling bottle bottle is selected peroxy-borate glass, has good intensity and stability.
In some preferred embodiments of the utility model, for improving dissolved oxygen, the aspect ratio of described body is 0.9:1.
The method of use of the cell cultures rolling bottle of the utility model is: the diameter of opening large vol cell cultures rolling bottle central authorities be 100mm bottleneck frosted bottleneck (promptly; Main bottleneck 12), adds glass cleaner or strong acid and clean deactivation, pour out scavenging solution; Residual with the purified water flushing to there not being scavenging solution; Carry out drip washing with water for injection, be inverted large vol cell cultures rolling bottle and dry, place 350 ℃ of removal pyrogens in the xeothermic baking oven.In super clean bench, be 100mm bottleneck frosted bottleneck sealing with diameter; Simultaneously; It is the pipe connection spiral cover of 45mm neck side direction bottleneck (opening for feed 13 and discharge port 14) that bottle both sides diameter is installed; Each spiral cover respectively has two passages, is respectively input and output material pipeline and the turnover gas hydrophobic filter of leading to bottleneck at the bottom of leading to bottle.Be positioned over steam sterilization cabinet and carry out 121 ℃, the 30min sterilization adds the serum-free protein-free medium through feed-pipe then, behind the access cell, places and carries out cell cultures on the rotary shaking table.According to the nutrient solution volume, suitable rotating speed is set, to guarantee that cell fully suspends.The large vol cell cultures rolling bottle of the utility model can be taken a sample fluid infusion easily through discharge pipe on the pipe connection spiral cover and feed pipe.Cell cultures is opened bottleneck, the very convenient cell culture fluid that promptly takes out after finishing.
In sum, the large vol cell cultures rolling bottle that the utility model provides, simple in structure, design ingeniously, install simply, through rotary shaking table, can fully cell suspension be got up, guarantee cell dissolved oxygen and growth, thereby reach the purpose that mass cell is cultivated.
Although the content of the utility model has been done detailed introduction through above-mentioned preferred embodiment, will be appreciated that above-mentioned description should not be considered to the restriction to the utility model.After those skilled in the art have read foregoing, for the multiple modification of the utility model with to substitute all will be conspicuous.Therefore, the protection domain of the utility model should be limited appended claim.
Claims (5)
1. large vol cell cultures rolling bottle is characterized in that this rolling bottle comprises:
Body (11);
Be arranged on the main bottleneck (12) of body center of top;
Be arranged on the opening for feed (13) of main bottleneck (12) sidepiece; And,
Be arranged on the discharge port (14) of main bottleneck (12) sidepiece, this discharge port (14) is the centrosymmetry setting with opening for feed (13) with main bottleneck (12);
Wherein, described opening for feed (13) is through the sealing of opening for feed pipe connection spiral cover, and this opening for feed pipe connection spiral cover comprises feed pipe and the inlet mouth drain water piping that runs through spiral cover; Described discharge port (14) is through the sealing of discharge port pipe connection spiral cover, and this discharge port pipe connection spiral cover comprises discharge pipe and the air outlet drain water piping that runs through spiral cover.
2. large vol cell cultures rolling bottle as claimed in claim 1 is characterized in that described body is a right cylinder, and the aspect ratio of body is 0.9 ~ 1:1.
3. according to claim 1 or claim 2 large vol cell cultures rolling bottle is characterized in that the bore of described main bottleneck (12) is greater than the bore of opening for feed (13) and discharge port (14).
4. large vol cell cultures rolling bottle as claimed in claim 3 is characterized in that described main bottleneck (12) is the frosted bottleneck, seals through bottle stopper.
5. large vol cell cultures rolling bottle as claimed in claim 4 is characterized in that described feed pipe, inlet mouth drain water piping, discharge pipe and air outlet drain water piping are silicone tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120540766 CN202430229U (en) | 2011-12-22 | 2011-12-22 | High-volume roller bottle for tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120540766 CN202430229U (en) | 2011-12-22 | 2011-12-22 | High-volume roller bottle for tissue culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202430229U true CN202430229U (en) | 2012-09-12 |
Family
ID=46779693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120540766 Expired - Fee Related CN202430229U (en) | 2011-12-22 | 2011-12-22 | High-volume roller bottle for tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202430229U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210189311A1 (en) * | 2018-05-16 | 2021-06-24 | aquila biolabs GmbH | Device and method for culturing cells in a shaking operation |
| CN113667601A (en) * | 2021-07-28 | 2021-11-19 | 江苏谱新生物医药有限公司 | Device and method for continuous culture and sampling of cells |
-
2011
- 2011-12-22 CN CN 201120540766 patent/CN202430229U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210189311A1 (en) * | 2018-05-16 | 2021-06-24 | aquila biolabs GmbH | Device and method for culturing cells in a shaking operation |
| CN113667601A (en) * | 2021-07-28 | 2021-11-19 | 江苏谱新生物医药有限公司 | Device and method for continuous culture and sampling of cells |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: SHANGHAI SAIJIN BIOMEDICAL CO., LTD. Free format text: FORMER OWNER: SHANGHAI KANDA BIOLOGICAL MEDICINE TECHNOLOGY CO., LTD. Effective date: 20130722 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20130722 Address after: 201203 Shanghai Zhangjiang hi tech Park Chunxiao Road No. 300 room 306 Patentee after: Shanghai Saijin Biomedical Co., Ltd. Address before: 201203 Shanghai Zhangjiang High Tech Park of Pudong New Area Chunxiao Road No. 300 Patentee before: Shanghai Kanda Biotechnology Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120912 Termination date: 20201222 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |