CN209872925U - Portable PCR real-time fluorescence detection system - Google Patents
Portable PCR real-time fluorescence detection system Download PDFInfo
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- CN209872925U CN209872925U CN201920519405.4U CN201920519405U CN209872925U CN 209872925 U CN209872925 U CN 209872925U CN 201920519405 U CN201920519405 U CN 201920519405U CN 209872925 U CN209872925 U CN 209872925U
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Abstract
The utility model discloses a portable PCR real-time fluorescence detection system, which comprises a laser optical system, a fluorescence detection optical system, a temperature circulating system and a control system, a temperature control device of the temperature circulating system and a reaction tank arranged on the temperature control device; the laser optical system comprises an LED light source, a four-color filter of a light-emitting system, a first focusing lens and a second focusing lens which are sequentially arranged along the transmission direction of a light path, an emission optical fiber is arranged between the laser optical system and the reaction tank, and emergent light of the second focusing lens is transmitted to the reaction tank through the emission optical fiber; the fluorescence detection optical system comprises a beam splitting lens, a four-color filter of a lighting system, a third focusing lens and an optical detection element which are sequentially arranged along the transmission direction of a light path, and a receiving optical fiber is arranged between the fluorescence detection optical system and the reaction tank. The utility model provides a portable PCR real-time fluorescence detection system, it is small, conveniently carry.
Description
Technical Field
The utility model relates to a biological detection technical field, in particular to portable PCR real-time fluorescence detection system.
Background
Polymerase Chain Reaction (PCR) is a molecular biology technology for amplifying and amplifying specific DNA fragments, which can be regarded as special DNA amplification outside organisms, and a temperature cycle period is formed by several steps of reactions such as high-temperature denaturation, low-temperature annealing, moderate-temperature extension and the like, so that the purpose can be rapidly amplified, and the PCR amplification technology has the characteristics of strong specificity, high sensitivity, simplicity and convenience in operation, time saving and the like, and is a major innovation of a gene amplification technology.
The PCR detection system in the prior art has the advantages of large volume, heavy weight, inconvenient carrying, large power consumption, difficult outdoor operation and long experiment time.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a portable PCR real-time fluorescence detecting system, compact structure, it is small, conveniently carry.
Based on the above problem, the utility model provides a technical scheme is:
the portable PCR real-time fluorescence detection system comprises a laser optical system, a fluorescence detection optical system, a temperature circulating system and a control system, wherein the laser optical system, the fluorescence detection optical system and the temperature circulating system are respectively and electrically connected with the control system; the laser optical system comprises an LED light source, a four-color filter of a light-emitting system, a first focusing lens and a second focusing lens which are sequentially arranged along the transmission direction of a light path, an emission optical fiber is arranged between the laser optical system and the reaction tank, and emergent light of the second focusing lens is transmitted to the reaction tank through the emission optical fiber; the fluorescence detection optical system comprises a beam splitting lens, a lighting system four-color filter, a third focusing lens and an optical detection element which are sequentially arranged along the transmission direction of a light path, and a receiving optical fiber is arranged between the fluorescence detection optical system and the reaction tank so as to transmit emitted light generated by exciting a reagent in the reaction tank to the beam splitting lens.
In some embodiments, the temperature control device includes a semiconductor chilling plate connected to the driving circuit, a heat sink disposed below the semiconductor chilling plate, a fan disposed at a lower end of the heat sink, and a temperature sensor disposed on the reaction tank, and the reaction tank is disposed above the semiconductor chilling plate.
In some of these embodiments, the lower end of the heat sink is provided with four legs.
In some embodiments, the laser optical system and the fluorescence detection optical system are disposed at a side portion of the temperature control device and arranged in an up-and-down manner.
In some embodiments, the temperature control device and the fluorescence detection optical system are mounted on a base.
In some of these embodiments, the laser optical system further comprises a first housing, the first housing being cube-shaped; the fluorescence detection optical system further comprises a second housing, and the second housing is cube-shaped.
In some embodiments, the first and second shells have a side length of 30-50 mm.
In some of these embodiments, the optical detection element is a photodiode, photomultiplier tube, or avalanche photodiode.
In some of these embodiments, the LED light source comprises four monochromatic LED lights.
Compared with the prior art, the utility model has the advantages that:
adopt the technical scheme of the utility model, laser optical system and fluorescence detection optical system adopt luminous beam four unifications, and daylighting beam one closes four structures, adopt optical transmission, whole detecting instrument's compact structure, and is small, conveniently carries.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the description of the embodiments are briefly introduced below, the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the portable PCR real-time fluorescence detection system of the present invention;
fig. 2 is a schematic view of the working principle of the embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a temperature cycle system according to an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a laser optical system according to an embodiment of the present invention;
FIG. 5 is a cross-sectional view taken along line A-A of FIG. 4;
FIG. 6 is a schematic structural diagram of a fluorescence optical detection system according to an embodiment of the present invention;
wherein:
1. a laser optical system; 1-1, LED light source; 1-2, a four-color filter of a light-emitting system; 1-3, a first focusing lens; 1-4, a second focusing lens; 1-5, a first fixed seat; 1-6, a first fixing plate; 1-7, a second fixing plate; 1-8, a light source fixing seat; 1-9, a first separator;
2. a fluorescence detection optical system; 2-1, a beam splitting lens; 2-2, a lighting system four-color filter; 2-3, a third focusing lens; 2-4, an optical detection element; 2-5, a second fixed seat; 2-6, a third fixing plate; 2-7, a fourth fixing plate; 2-8, a second separator;
3. a temperature cycling system; 3-1, a reaction tank; 3-2, semiconductor refrigerating sheets; 3-3, a radiator; 3-4, a fan; 3-5, support legs;
4. a control system;
5. an emission optical fiber;
6. receiving an optical fiber;
7. a base.
Detailed Description
The above-described scheme is further illustrated below with reference to specific examples. It should be understood that these examples are for illustrative purposes and are not intended to limit the scope of the present invention. The implementation conditions used in the examples can be further adjusted according to the conditions of a specific manufacturer, and the implementation conditions not specified are generally the conditions in the conventional work.
Referring to fig. 1-3, for the structural schematic diagram of the embodiment of the present invention, a portable PCR real-time fluorescence detection system is provided, which includes a laser optical system 1, a fluorescence detection optical system 2, a temperature cycle system 3, and a control device 4, wherein the laser optical system 1, the fluorescence detection optical system 2, and the temperature cycle system 3 are respectively electrically connected to the control system 4.
The temperature circulating system 3 comprises a temperature control device and a reaction tank 3-1 arranged on the temperature control device, the temperature control device comprises a semiconductor chilling plate 3-2 connected to a driving circuit, a radiator 3-3 arranged below the semiconductor chilling plate 3-2, a fan arranged at the lower end of the radiator 3-3 and a temperature sensor arranged on the reaction tank 3-1, the temperature sensor is in signal connection with a control device 4 to feed back the temperature of the reaction tank in real time, and the reaction tank 3-1 is arranged above the semiconductor chilling plate 3-2. The semiconductor refrigerating sheet 3-2 supplies power to heat and cool the reaction tank 3-1, and the heating and cooling processes of the semiconductor refrigerating sheet 3-2 are completed by the aid of the radiator 3-3 and the fan 3-4.
The laser optical system 1 comprises an LED light source 1-1, a light-emitting system four-color optical filter 1-2, a first focusing lens 1-3 and a second focusing lens 1-4 which are sequentially arranged along the transmission direction of a light path, an emitting optical fiber 5 is arranged between the laser optical system 1 and the reaction tank 3-1, the emergent light of the second focusing lens 1-4 is transmitted to the reaction tank 3-1 through the emitting optical fiber 5, and the LED light source 1-1 comprises four monochromatic LED lamps. The monochromatic light emitted by one monochromatic LED lamp is focused by the first focusing lens 1-3 and the second focusing lens 1-4 through the corresponding optical filter to be finally coupled into the emission optical fiber 5 for transmission, so that the excitation light purity is improved.
Referring to fig. 4 and 5, the laser optical system 1 further includes a first fixing base 1-5, a first fixing plate 1-6, a second fixing plate 1-7, and a light source fixing base 1-8 disposed at both sides of the first fixing base 1-5 in the first direction, the first fixing base 1-5, the first fixing plate 1-6, and the second fixing plate 1-7 are respectively and correspondingly provided with slots for fixing a second focusing lens 1-4, first partition plates 1-9 are further disposed at positions of the light emitting system four-color filter 1-2 and the first focusing lens 1-3, the first partition plates 1-9, the first fixing base 1-5, the first fixing plate 1-6, and the second fixing plate 1-7 are respectively and correspondingly provided with slots for fixing the light emitting system four-color filter 1-2 and the first focusing lens 1-3, the light source fixing seats 1-8 are used for fixing four monochromatic LED lamps.
The fluorescence detection optical system 2 comprises a beam splitting lens 2-1, a four-color filter 2-2 of a lighting system, a third focusing lens 2-3 (the name is different from that of the focusing lens of the light-emitting system, and the third focusing lens is not recommended to be deleted) and an optical detection element 2-4 which are sequentially arranged along the direction of a light path, wherein a receiving optical fiber 6 is arranged between the fluorescence detection optical system 2 and the reaction cell 3-1 so as to transmit emitted light generated by exciting a reagent in the reaction cell 3-1 to the beam splitting lens 2-1. The optical detection elements 2-4 are photodiodes, photomultiplier tubes or avalanche photodiodes. After the emitted light of the excited reagent in the reaction tank 3-1 reaches the beam splitting lens 2-1, the stray light signals are filtered by the corresponding light collecting system optical filter 2-2, then are focused on the light detection element 2-4 by the third focusing lens 2-3 for photoelectric conversion, and after the signals are amplified, the signals are transmitted to the control system 4 for continuous data acquisition, and the fluorescence detection is completed.
Referring to fig. 6, the fixing manner of each component in the fluorescence detection optical system 2 is similar to that of the laser optical system 1, and a second fixing base 2-5, a third fixing plate 2-6, a fourth fixing plate 2-7, and a second partition plate 2-8 are provided, a corresponding slot fixing beam splitting lens 2-1 is provided on the second fixing base 2-5, the third fixing plate 2-6, and the fourth fixing plate 2-7, and a corresponding slot is provided on the second fixing base 2-5, the third fixing plate 2-6, the fourth fixing plate 2-7, and the second partition plate 2-8 to fix the four-color filter 2-2, the third focusing lens 2-3, and the optical detection element 2-4 of the lighting system.
The control system 4 comprises a fluorescence control circuit board and a thermal cycle control circuit board, the fluorescence control circuit board controls the four monochromatic LED lamps to be turned on or turned off through the high and low levels of the pins of the single chip microcomputer, the multi-color excitation of the sample is controlled, when the LED lamps with the corresponding colors are turned on, the optical detection elements at the corresponding positions in the fluorescence detection optical system start to collect fluorescence, and when the corresponding LED lamps are all turned on and turned off, the four-color fluorescence detection optical system also synchronously completes detection. The thermal cycle control circuit board adjusts the amplitude and the voltage direction of the power supply voltage of the semiconductor refrigeration sheet 3-2 in real time according to the real-time temperature value returned by the temperature sensor on the reaction tank 3-1, and starts the fan 3-4 during cooling to assist the cooling process of the reaction tank 3-1, so that the temperature of the reaction tank 3-1 is subjected to temperature rise and fall cycles according to a set thermal cycle program.
In this example, four support legs are provided at the lower end of the heat sink 3-3 to make the heat sink 3-3 overhead for heat dissipation.
The laser optical system 1 and the fluorescence detection optical system 2 are arranged at the side part of the temperature control device and are arranged up and down.
The fluorescence detection device is also provided with a base 7, and the temperature control device, the fluorescence detection optical system 2 and the control system 4 are arranged on the base 7.
In this embodiment, the laser optical system 1 further includes a first housing, and the fluorescence detection optical system 2 further includes a second housing, where the first housing and the second housing are cubic, and a side length of the cube is 30-50 mm, and in this embodiment, the side length of the cube is 40mm, so that the whole detection system is convenient to miniaturize and carry.
The utility model discloses a theory of operation does: light emitted by the first monochromatic LED lamp is filtered by the corresponding light-emitting system light filter 1-2, is primarily focused by the first focusing lens 1-3 lines to reach the second focusing lens 1-4, is finally coupled to the emission optical fiber 5, and is finally transmitted to the reaction tank 3-1, so that the excitation process is completed. When the reagent is subjected to PCR reaction at the reaction cell 3-1, the reagent emits light of another specific wavelength range of a longer wavelength band when irradiated with excitation light of a specific wavelength range. The emitted light of the specific waveband is received by a receiving optical fiber fixed on the side wall of the reaction tank 3-1 and then transmitted to a fluorescence detection optical system 2, after the fluorescence signal is received by the fluorescence detection optical system 2, after being split by a beam splitting lens 2-1, the stray light signal is filtered by a corresponding light collection system optical filter 2-2, and then the stray light signal is focused by a third focusing lens 2-3 onto a light sensing surface of an optical detection element 2-4 to realize the conversion of the photoelectric signal. The converted weak electric signal is transmitted to the circuit board after being preliminarily amplified, and the first path of fluorescence collection process is completed. And so on, the implementation processes of the second path, the third path and the fourth path are completely consistent, and only the control pins used by the singlechip during working are different. The process is completed only by hundreds of milliseconds or even dozens of milliseconds, the acquisition time is extremely short, the acquisition time can be greatly saved, and the real-time performance of signal acquisition is also ensured.
The above embodiments are only for illustrating the technical conception and the features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to implement the present invention, which should not limit the scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered by the protection scope of the present invention.
Claims (9)
1. Portable PCR real-time fluorescence detection system, including laser optical system (1), fluorescence detection optical system (2), temperature cycle system (3), and control system (4), laser optical system (1), fluorescence detection optical system (2), temperature cycle system (3) respectively with control system (4) electricity is connected, characterized in that: the temperature circulating system (3) comprises a temperature control device and a reaction tank (3-1) arranged on the temperature control device; the laser optical system (1) comprises an LED light source (1-1), a light-emitting system four-color filter (1-2), a first focusing lens (1-3) and a second focusing lens (1-4) which are sequentially arranged along the transmission direction of a light path, an emission optical fiber (5) is arranged between the laser optical system (1) and the reaction tank (3-1), and emergent light of the second focusing lens (1-4) is transmitted to the reaction tank (3-1) through the emission optical fiber (5); the fluorescence detection optical system (2) comprises a beam splitting lens (2-1), a lighting system four-color filter (2-2), a third focusing lens (2-3) and an optical detection element (2-4) which are sequentially arranged along the transmission direction of a light path, wherein a receiving optical fiber (6) is arranged between the fluorescence detection optical system (2) and the reaction tank (3-1) so as to transmit emitted light generated by exciting a reagent in the reaction tank (3-1) to the beam splitting lens (2-1).
2. The portable PCR real-time fluorescence detection system according to claim 1, wherein: the temperature control device comprises a semiconductor refrigeration piece (3-2) connected to a driving circuit, a radiator (3-3) arranged below the semiconductor refrigeration piece (3-2), a fan (3-4) arranged at the lower end of the radiator (3-3), and a temperature sensor arranged on the reaction tank (3-1), wherein the reaction tank (3-1) is arranged above the semiconductor refrigeration piece (3-2).
3. The portable PCR real-time fluorescence detection system according to claim 2, wherein: the lower end of the radiator (3-3) is provided with four support legs (3-5).
4. The portable PCR real-time fluorescence detection system according to claim 2, wherein: the laser optical system (1) and the fluorescence detection optical system (2) are arranged at the side part of the temperature control device and are arranged up and down.
5. The portable PCR real-time fluorescence detection system of claim 4, wherein: the fluorescent detection device is characterized by further comprising a base (7), wherein the temperature control device and the fluorescent detection optical system (2) are mounted on the base (7).
6. The portable PCR real-time fluorescence detection system according to claim 1, wherein: the laser optical system (1) further comprises a first shell, and the first shell is cubic; the fluorescence detection optical system (2) further comprises a second housing, and the second housing is cube-shaped.
7. The portable PCR real-time fluorescence detection system of claim 6, wherein: the side lengths of the first shell and the second shell are 30-50 mm.
8. The portable PCR real-time fluorescence detection system according to claim 1, wherein: the optical detection elements (2-4) are photodiodes, photomultiplier tubes or avalanche photodiodes.
9. The portable PCR real-time fluorescence detection system according to claim 1, wherein: the LED light source (1-1) comprises four monochromatic LED lamps.
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| CN201920519405.4U CN209872925U (en) | 2019-04-17 | 2019-04-17 | Portable PCR real-time fluorescence detection system |
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| CN201920519405.4U CN209872925U (en) | 2019-04-17 | 2019-04-17 | Portable PCR real-time fluorescence detection system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109957496A (en) * | 2019-04-17 | 2019-07-02 | 苏州雅睿生物技术有限公司 | Portable PC R real-time fluorescence detection system |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109957496A (en) * | 2019-04-17 | 2019-07-02 | 苏州雅睿生物技术有限公司 | Portable PC R real-time fluorescence detection system |
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Address after: 215000 rooms 101 and 201, C7 building, biomedical industrial park, 218 Xinghu street, Suzhou Industrial Park, Jiangsu Province Patentee after: Suzhou Yarui Biotechnology Co.,Ltd. Address before: 215000 rooms 101 and 201, building C7, bio nano technology park, No. 218, Xinghu street, Suzhou Industrial Park, Suzhou City, Jiangsu Province Patentee before: SUZHOU MOLARRAY BIOTECHNOLOGY Co.,Ltd. |