CN208218878U - Cell culture vial - Google Patents
Cell culture vial Download PDFInfo
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- CN208218878U CN208218878U CN201721849395.8U CN201721849395U CN208218878U CN 208218878 U CN208218878 U CN 208218878U CN 201721849395 U CN201721849395 U CN 201721849395U CN 208218878 U CN208218878 U CN 208218878U
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- cell culture
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Abstract
The utility model discloses a kind of cell culture vials.The cell culture vial includes glass body, glass bottle neck and bottle cap, the glass body is in quadrangular shape, the glass body has culture chamber, the culture chamber has two groups of opposite inner walls, the sealed bottom of the glass body, the top of the glass body has the opening for being connected to the culture chamber, the top of the glass bottle neck has bottleneck, the bottom of the glass bottle neck is connected to the top of the glass body, the bottleneck and the open communication, the bottle cap detachable connection is in the glass bottle neck.The cell culture vial can effectively solve the problem that the problem that dissolved oxygen is insufficient, amplification culture is difficult.
Description
Technical field
The utility model relates to a kind of cell culture vials.
Background technique
With the rapid development of biopharmaceutical industry, the recombinant protein that mammalian cell suspension culture techniques become mainstream is raw
Production technology.Mammalian cell, which suspends, to be cultivated usually using disposable plastic Tissue Culture Flask, because of this culture bottle safety
High, mature, bottle cap have sealing cover, ventilating cover etc. available；The use cost of opposite batch cultur bottle is also very high,
Heavy financial burden is not only brought to researcher, also results in the serious wasting of resources in research and production process；And glass is thin
Born of the same parents' culture bottle high pressure sterilization, use cost can substantially reduce repeatedly, but glass spinner flask used at present there is dissolved oxygens not
The problems such as foot, difficult amplification.
Utility model content
Based on this, it is necessary to provide a kind of problem and cost that can effectively solve the problem that dissolved oxygen is insufficient, amplification culture is difficult
Low cell culture vial.
A kind of cell culture vial, including glass body, glass bottle neck and bottle cap, the glass body are in quadrangular
Shape, the glass body have culture chamber, and the culture chamber has two groups of opposite inner walls, and the bottom of the glass body is close
Envelope, the top of the glass body have the opening for being connected to the culture chamber, and the top of the glass bottle neck, which has, to be connected to
The bottleneck of the opening, the bottom of the glass bottle neck are connected to the top of the glass body, the detachable company of bottle cap
It is connected to the glass bottle neck.
The size uniformity of each inner wall of the culture chamber causes in one of the embodiments,.
The glass body includes the side plate of rectangular bottom plate and strip, the side in one of the embodiments,
The quantity of plate is four, and the one side edge of four side plates is connected to four edges of the bottom plate, and adjacent is described
The edge of side plate connects, and the culture chamber is surrounded between four side plates and the bottom plate, and the bottom of the glass bottle neck connects
It is connected to the edge of four side plates.
The side length of the bottom plate is 6.5cm-8cm in one of the embodiments, and the length of the side plate is 18cm-
22cm, the width of the side plate is consistent with the side length of the bottom plate, and the shorter side edge of the side plate is connect with the bottom plate.
The internal diameter of the bottleneck is 3cm in one of the embodiments, and the outer diameter of the bottleneck is 4.1cm-4.5cm.
The top of the glass bottle neck has external screw thread in one of the embodiments, and the inner wall of the bottle cap has interior
Screw thread, the bottle cap are connect with the screw top of the glass bottle neck.
The bottom of the glass bottle neck and the glass body rounding off in one of the embodiments,.
It in one of the embodiments, further include baffle, the baffle is triangular in shape, and the baffle is located at the culture chamber
Interior, the bottom edge edge of the baffle is connected to the bottom surface of the culture chamber.
The bottom edge of the baffle is located at one of diagonal line of the culture chamber bottom surface in one of the embodiments,
On.
The bottle cap is venting bottle cap in one of the embodiments,.
Above-mentioned cell culture vial is made by setting glass body and the culture chamber with two groups of opposite inner walls
Its culture for being more suitable for suspension cell.When using cell culture vial rock platform carry out cell culture when, because cell train
The presence of the corner angle of the culture cavity wall of vial is supported, so that the shock degree of cell liquid increases, cell can be maintained to be in and suspended
State, thus achieve the purpose that suspend culture, be better than using cell density when cell culture vial and Cell viability
Conical flask does not have an impact the transfection of cell and expression process using cell culture vial, and cell culture amplification process
Simply, easy to operate, pollution risk is small.Above-mentioned cell culture vial is manufactured simply, at low cost.Above-mentioned cell culture glass
Glass bottle is transformed and optimizes to the shape of glass body, it is made to be more suitable for the culture of suspension cell.
Above-mentioned cell culture vial increases one piece of triangular baffle in the bottom surface of culture chamber, so that dissolved oxygen increases.
Above-mentioned cell culture vial, bottle cap are venting bottle cap, so that can guarantee cell in the case where obstructed pure oxygen
Normal growth.
Detailed description of the invention
Fig. 1 is that cell culture vial described in an embodiment is free of baffle schematic diagram；
Fig. 2 is cell culture vial schematic diagram described in another embodiment.
Description of symbols
10, cell culture vial；100, glass body；110, culture chamber；120, bottom plate；130, side plate；200, glass
Bottleneck；300, bottle cap；400, baffle.
Specific embodiment
The utility model is more fully retouched below with reference to relevant drawings for the ease of understanding the utility model,
It states.The preferred embodiment of the utility model is given in attached drawing.But the utility model can come in many different forms in fact
It is existing, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is makes public affairs to the utility model
The understanding for opening content is more thorough and comprehensive.
It should be noted that it can directly on the other element when element is referred to as " being fixed on " another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it, which can be, is directly connected to
To another element or it may be simultaneously present centering elements.
Unless otherwise defined, all technical and scientific terms used herein are led with the technology for belonging to the utility model
The normally understood meaning of the technical staff in domain is identical.Terminology used in the description of the utility model herein only be
The purpose of description specific embodiment, it is not intended that in limitation the utility model.Term as used herein "and/or" includes
Any and all combinations of one or more related listed items.
Shown in Figure 1, the present embodiment relates to a kind of cell culture vial 10.The cell culture vial 10 includes
Glass body 100, glass bottle neck 200 and bottle cap 300.
Shown in Figure 1, glass body 100 is in quadrangular shape.Glass body 100 has culture chamber 110.Culture chamber 110
With two groups of opposite inner walls, two groups of opposite inner walls namely the two of every group inner walls are opposite.
The sealed bottom of glass body 100.The top of glass body 100 has the opening for being connected to culture chamber 110.Glass
The top of bottleneck 200 has bottleneck.The bottom of glass bottle neck 200 is connected to the top of glass body 100.Bottleneck and opening connect
It is logical.
300 detachable connection of bottle cap is in glass bottle neck 200.
Further, in one embodiment, the size uniformity of each inner wall of culture chamber 110 causes.
Further, shown in Figure 1, in one embodiment, glass body 100 include rectangular bottom plate 120 with
And the side plate 130 of strip.The quantity of side plate 130 is four.The one side edge of four side plates 130 is connected to bottom plate 120
Four edges.The edge of adjacent side plate 130 connects.Culture chamber 110 is surrounded between four side plates 130 and bottom plate 120.Glass
The bottom of bottleneck 200 is connected to the edge of four side plates 130.
Preferably, in one embodiment, the side length of bottom plate 120 is 6.5cm-8cm.The length of side plate 130 is 18cm-
22cm.The width of side plate 130 is consistent with the side length of bottom plate 120, and the shorter side edge of side plate 130 is connect with bottom plate 120.When thin
When the volume of born of the same parents' culture vial 10 is 1L, the side length of bottom plate 120 is 8cm, and the length of side plate 130 is 22cm.Work as cell culture
When the volume of vial 10 is 500mL, the side length of bottom plate 120 is 6.5cm.The length of side plate 130 is 18cm.
In one embodiment, the internal diameter of bottleneck is 3cm, and the outer diameter of bottleneck is 4.1cm-4.5cm.When cell culture glass
When the volume of bottle 10 is 1L, the internal diameter of bottleneck is 3cm, outer diameter 4.5cm.When the volume of cell culture vial 10 is 500mL
When, the internal diameter of bottleneck is 3cm, outer diameter 4.1cm.
Preferably, in one embodiment, the top of glass bottle neck 200 has external screw thread, and the inner wall of bottle cap 300 has interior
Screw thread, bottle cap 300 are connect with the screw top of glass bottle neck 200.
In one embodiment, the bottom of glass bottle neck 200 and 100 rounding off of glass body, to increase cell culture
The round and smooth degree of 10 outer surface of vial avoids corner angle are excessive from being inconvenient to be affectedly bashful, operate.
Shown in Figure 2, in one embodiment, above-mentioned cell culture vial 10 further includes baffle 400.Baffle 400
It is triangular in shape, such as equilateral triangle, isosceles triangle or other triangles etc..Baffle 400 is located in culture chamber, baffle 400
Bottom edge edge be connected to the bottom surface of culture chamber.
In one embodiment, the bottom edge of baffle 400 is located on one of diagonal line of the culture chamber bottom surface.
In one embodiment, bottle cap 300 is venting bottle cap.Above-mentioned cell culture vial 10, bottle cap 300 are ventilative
Bottle cap, so that can guarantee the normal growth of cell in the case where obstructed pure oxygen.
Above-mentioned cell culture vial 10 when in use, can be placed directly in ring rail and rock in system, progress CHO,
The isocellular culture of HEK293E.Compared with traditional conical flask, the dissolved oxygen efficiency of cell culture vial 10 is higher than conical flask,
The foam of generation is less than conical flask.In different time sections measurement CHO-DG44 cell in cell culture vial 10 and conical flask
Dissolved oxygen amount, find that the dissolved oxygen amount in cell culture vial 10 is obviously high through NOVA pHOx analysis-e/or determining dissolved oxygen content
In conical flask, specific data are shown in Table 1.
Dissolved oxygen amount of the 1 CHO-DG44 cell of table in cell culture vial 10 and conical flask
When suspension cell is cultivated in cell culture vial 10 and conical flask, cell is in baffled cell culture
Growth conditions in vial 10 are better than conical flask, and specific data are shown in Table 2.Table 2 is CHO-DG44 cell respectively in the glass side 1L
It is cultivated in bottle and 1L triangular flask, is counted for 24 hours, after 48h, 72h, 96h, 144h, 168h to cell in culture.Data
Show that the effect that CHO--DG44 cell is cultivated in glass square vase is better than conical flask.
Stand density of the 2 CHO-DG44 cell of table in glass square vase and conical flask
When cell is cultivated in the cell culture vial 10 of venting bottle cap, cell growth state is with TPP bottle without bright
Significant difference is other.Compare HEK cell in the cell culture vial 10 of 1L and the upgrowth situation of 1L TPP bottle simultaneously, takes different time
The cell of point culture is counted.Table 3 is HEK cell growing state in two kinds of culture bottles.
Stand density of the 3 HEK293E cell of table in square vase glass jar and TPP bottles
Cell culture vial 10 is placed in after ring rail rocks, and can realize amplification process by rocking track and state modulator
The consistency of middle cell culture condition, it is not necessary to grope condition again as traditional approach is directed to extensive expression again.
By taking HEK293E cell is cultivated in cell culture vial 10 as an example:
1) HEK293E cell recovery is cultivated in 50mL culture tube.
2) cell expansion culture
2-1) by the HEK293E cell expansion of step 1) into 250mL cell culture vial 10 and stirring-type vial.
2-2) by the HEK293E cell expansion of step 2-1) to 500mL cell culture vial 10 and stirring-type vial
In.
3) step 2) is pressed into cell density 3.5 × 106Cells/mL is seeded in 1L cell culture vial 10, culture medium
For Pro293s-CDM, volume of culture 300mL.Cell culture vial 10 is placed in ring rail shaking table and is carried out with the revolving speed of 120rpm
Cell culture, cultivation temperature are 37 DEG C.
4) step 2) is pressed into cell density 3.5 × 106Cells/mL is seeded to 1L stirring-type vial.Rotating speed of agitator is set
For 80rpm, culture bottle is put in 37 DEG C of incubators and is cultivated.
5) step 3) and step 4) culture cell carry out cell viable count after 50h, 75h, 125h, 150h respectively,
And it is depicted as cell growth curve.
6) according to above scheme, by the cell of step 1) with 3.5 × 106Cells/mL be extended to respectively 250mL,
In 500mL, 1L cell culture vial 10,250h is cultivated, volume of culture is the 40% of culture bottle volume, and is being cultivated respectively
Cell passage is carried out after 72h and 168h.
According to above scheme, viable count carried out to step 6) cell, gate time be respectively cell culture for 24 hours,
72h,96h,120h,144h,168h,192h,216h,240h.The result shows that the growth of HEK cell is unaffected.
Above-mentioned cell culture vial 10 passes through setting glass body 100 and the culture with two groups of opposite inner walls
Chamber 110 makes it be more suitable for the culture of suspension cell.When using cell culture vial 10 rocking platform carry out cell culture
When, because of the presence of the corner angle of 110 inner wall of culture chamber of cell culture vial 10, so that the shock degree of cell liquid increases, it can
It maintains cell to be in the state to suspend, to achieve the purpose that the culture that suspends, is grown using cell when cell culture vial 10
Density and Cell viability are better than conical flask, do not generate shadow to the transfection of cell and expression process using cell culture vial 10
It rings, and cell culture amplification process is simple, easy to operate, pollution risk is small.Above-mentioned cell culture vial 10, to glass body
100 shape is transformed and optimizes, it is made to be more suitable for the culture of suspension cell.
Above-mentioned cell culture vial 10 increases one piece of triangular baffle 400 in the bottom surface of culture chamber, so that dissolved oxygen increases
Add.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
Above-described embodiments merely represent several embodiments of the utility model, the description thereof is more specific and detailed,
But it should not be understood as limiting the scope of the patent of the utility model.It should be pointed out that for the common of this field
For technical staff, without departing from the concept of the premise utility, various modifications and improvements can be made, these all belong to
In the protection scope of the utility model.Therefore, the scope of protection shall be subject to the appended claims for the utility model patent.
Claims (10)
1. a kind of cell culture vial, which is characterized in that including glass body, glass bottle neck and bottle cap, the vial
Body is in quadrangular shape, and the glass body has culture chamber, and the culture chamber has two groups of opposite inner walls, the glass body
Sealed bottom, the top of the glass body has the opening for being connected to the culture chamber, the top tool of the glass bottle neck
There is the bottleneck for being connected to the opening, the bottom of the glass bottle neck is connected to the top of the glass body, and the bottle cap can
It is detachably connected in the glass bottle neck.
2. cell culture vial according to claim 1, which is characterized in that the size of each inner wall of the culture chamber
It is consistent.
3. cell culture vial according to claim 2, which is characterized in that the glass body includes rectangular bottom plate
And the side plate of strip, the quantity of the side plate are four, the one side edge of four side plates is connected to the bottom
Four edges of plate, the edge connection of the adjacent side plate, surround the culture between four side plates and the bottom plate
Chamber, the bottom of the glass bottle neck are connected to the edge of four side plates.
4. cell culture vial according to claim 3, which is characterized in that the side length of the bottom plate is 6.5cm-8cm,
The length of the side plate is 18cm-22cm, and the width of the side plate is consistent with the side length of the bottom plate, the shorter side of the side plate
Side edge is connect with the bottom plate.
5. cell culture vial according to any one of claims 1-4, which is characterized in that the internal diameter of the bottleneck is
3cm, the outer diameter of the bottleneck are 4.1cm-4.5cm.
6. cell culture vial according to any one of claims 1-4, which is characterized in that the top of the glass bottle neck
Portion has external screw thread, and the inner wall of the bottle cap has internal screw thread, and the bottle cap is connect with the screw top of the glass bottle neck.
7. cell culture vial according to any one of claims 1-4, which is characterized in that the bottom of the glass bottle neck
Portion and the glass body rounding off.
8. cell culture vial according to any one of claims 1-4, which is characterized in that it further include baffle, it is described
Baffle is triangular in shape, and the baffle is located in the culture chamber, and the bottom edge edge of the baffle is connected to the bottom of the culture chamber
Face.
9. cell culture vial according to claim 8, which is characterized in that the bottom edge of the baffle is located at the culture
On one of diagonal line in bottom of chamber face.
10. cell culture vial according to any one of claims 1-4, which is characterized in that the bottle cap is ventilative
Bottle cap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721849395.8U CN208218878U (en) | 2017-12-26 | 2017-12-26 | Cell culture vial |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721849395.8U CN208218878U (en) | 2017-12-26 | 2017-12-26 | Cell culture vial |
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| Publication Number | Publication Date |
|---|---|
| CN208218878U true CN208218878U (en) | 2018-12-11 |
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ID=64532930
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| CN201721849395.8U Active CN208218878U (en) | 2017-12-26 | 2017-12-26 | Cell culture vial |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107974408A (en) * | 2017-12-26 | 2018-05-01 | 广州汉腾生物科技有限公司 | Cell culture vial |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107974408A (en) * | 2017-12-26 | 2018-05-01 | 广州汉腾生物科技有限公司 | Cell culture vial |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20191205 Address after: Room 511, floor 5, building a, Central Europe center, No. 2, South Lingnan Avenue, Lecong Town, Shunde District, Foshan City, Guangdong Province Co-patentee after: Cantonbio Co., Ltd. Patentee after: Foshan hanteng Biotechnology Co., Ltd Address before: 510000, Guangdong International Biological Island, Guangzhou Development Zone, No. three, No. 12, road third, layer 302 Patentee before: Cantonbio Co., Ltd. |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Cell culture glass bottle Effective date of registration: 20210120 Granted publication date: 20181211 Pledgee: Shunde Guangdong rural commercial bank Limited by Share Ltd. Daliang branch Pledgor: CANTONBIO Co.,Ltd.|Foshan hanteng Biotechnology Co.,Ltd. Registration number: Y2021980000562 |