CN113117051B - Anti-aging transdermal polypeptide preparation and preparation method thereof - Google Patents

Abstract

The application discloses an anti-aging transdermal polypeptide preparation and a preparation method thereof, and the anti-aging transdermal polypeptide preparation comprises the following components in percentage by weight: 1-50% of hydroxypropyl tetrahydropyrane triol, 0.1-5% of collagen peptide, 0.1-1.0% of adenosine, 0.1-0.5% of carnosine, 0.5-5.0% of transdermal peptide, 0.4-0.8% of p-hydroxyacetophenone and the balance of water. The anti-aging transdermal polypeptide preparation provided by the application has high skin absorption rate, can promote the synthesis of collagen, can effectively prevent glycosylation, inhibit lipid oxidation caused by free radicals and metal ions, can help the new collagen to increase the skin resistance, keeps the activity of new cells under the condition of the free radicals, and can effectively keep the anti-aging effect.

Description

A kind of anti-aging transdermal polypeptide preparation and preparation method thereof

technical field

The present application relates to the field of anti-aging preparations, more specifically, to an anti-aging transdermal polypeptide preparation and a preparation method thereof.

Background technique

Today, due to the aging society and consumers' constant pursuit of youth and beauty, the proportion of anti-aging skin care products in the cosmetics market is also increasing year by year, and consumers' demand for the safety and effectiveness of anti-aging cosmetics is also increasing. The continuous improvement and perfection of the research and development technology of anti-aging cosmetics.

On the one hand, different skin types and ages have different levels of absorption of anti-aging skin care products. On the other hand, there are countless anti-aging skin care products on the market with various ingredients. Most anti-aging skin care products have molecular weight Larger, it is difficult to be absorbed and utilized by the skin, which affects the anti-aging effect of the actual user.

Therefore, how to provide an anti-aging preparation that can be efficiently absorbed by the skin has become an urgent problem to be solved by those skilled in the art.

Contents of the invention

In order to solve the above technical problems, the present application provides an anti-aging transdermal polypeptide preparation, which can improve the absorption efficiency of the skin.

The technical scheme that this application provides is as follows:

The present application provides an anti-aging transdermal polypeptide preparation, which includes the following components by weight percentage: 1%-50% of hydroxypropyltetrahydropyranotriol, 0.1%-5% of collagen peptide, and 0.1% of adenosine -1.0%, carnosine 0.1%-0.5%, transdermal peptide 0.5%-5.0%, p-hydroxyacetophenone 0.4%-0.8% and the balance of water.

Preferably, the anti-aging transdermal polypeptide preparation includes the following components by weight percentage: hydroxypropyltetrahydropyrantriol 5%-50%, collagen peptide 0.5%-5%, adenosine 0.5%- 1.0%, carnosine 0.1%-0.5%, transdermal peptide 0.5%-5.0%, p-hydroxyacetophenone 0.4%-0.8% and the rest of water.

In another preferred manner, the anti-aging transdermal polypeptide preparation includes the following components by weight percentage: 10%-50% of hydroxypropyl tetrahydropyranotriol, 0.5%-5% of collagen peptide, Adenosine 0.6%-1.0%, carnosine 0.2%-0.5%, transdermal peptide 1.0%-5.0%, p-hydroxyacetophenone 0.4%-0.7% and the rest of water.

In another preferred manner, the anti-aging transdermal polypeptide preparation includes the following components by weight percentage: 20%-50% of hydroxypropyl tetrahydropyranotriol, 0.5%-5% of collagen peptide, Adenosine 0.6%-1.0%, carnosine 0.2%-0.5%, transdermal peptide 1.5%-5.0%, p-hydroxyacetophenone 0.4%-0.7% and the rest of water.

Furthermore, in a preferred mode of the present invention, the anti-aging transdermal polypeptide preparation is a liposomal nano preparation.

Furthermore, in a preferred mode of the present invention, the average particle size of the anti-aging transdermal polypeptide preparation is 10-60 nm.

Preferably, the average particle size of the anti-aging transdermal polypeptide preparation is 10-50 nm.

The present application also provides a preparation method of an anti-aging transdermal polypeptide preparation for preparing the above-mentioned anti-aging transdermal polypeptide preparation, comprising the following steps:

Mixing hydroxypropyltetrahydropyrantriol, collagen peptide and deionized water evenly to prepare a hydroxypropyltetrahydropyrantriol mixed solution;

Mix and stir the emulsifier, glycerin and oil in proportion to obtain a lecithin mixture; add adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone to the hydroxypropyltetrahydropyranotriol in proportion and mix Mix in the liquid evenly to prepare the polypeptide mixed liquid;

The polypeptide mixture is added to the lecithin mixture, and two-stage high-pressure shear homogenization is carried out to obtain an anti-aging transdermal polypeptide preparation.

Further, in a preferred mode of the present invention, the specific components of the hydroxypropyltetrahydropyrantriol mixture include: hydroxypropyltetrahydropyrantriol with a mass concentration of 1%-55% , a collagen peptide with a mass concentration of 0.1%-5%.

Preferably, the specific components of the hydroxypropyltetrahydropyrantriol mixture include: hydroxypropyltetrahydropyrantriol with a mass concentration of 10%-55%, and hydroxypropyltetrahydropyrantriol with a mass concentration of 0.5%-5%. Collagen Peptides.

Furthermore, in a preferred mode of the present invention, the specific details of the step "add the polypeptide mixture to the lecithin mixture, and perform two-stage high-pressure shear homogenization to prepare an anti-aging transdermal polypeptide preparation" The conditions are: the first-stage homogeneous pressure is 20-60Mpa, the temperature is 10-35°C, the shear speed is 10-500r/min, the second-stage homogeneous pressure is 60-110Mpa, the temperature is 15-40°C, the shear The cutting speed is 10-500r/min.

Preferably, the speed of the first high-pressure shear is 40-500 r/min, and the speed of the second high-pressure shear is 60-500 r/min.

Preferably, the homogenization time for the first time is 5-40min, and the homogenization time for the second time is 10-30min.

Preferably, the two-stage high-pressure shear homogenizer is a two-stage high-pressure shear micro-jet homogenizer, and the flow rate of the two-stage micro-jet is 100-500 ml/min.

In another preferred manner, the first-stage micro-jet flow rate is 150-500ml/min, and the second-stage micro-jet flow rate is 250-500ml/min.

In another preferred manner, the first-stage micro-jet flow rate is 200-500ml/min, and the second-stage micro-jet flow rate is 280-500ml/min.

Further, in a preferred mode of the present invention, the emulsifier in the step of "mixing and stirring the emulsifier, glycerin and oil in proportion to uniformly prepare the lecithin mixture" is lecithin.

Further, in a preferred mode of the present invention, the oil in the step "mixing and stirring the emulsifier, glycerin, and oil in proportion to uniformly prepare the lecithin mixture" is squalane.

Further, in a preferred mode of the present invention, the specific added mass of the emulsifier, the glycerin and the oil in the step "mix and stir the emulsifier, glycerin and oil in proportion to uniformly prepare the lecithin mixture" The ratio is: 1:(1-4):5.

Preferably, the specific mass ratio of the emulsifier, the glycerin and the oil added in the step "mixing and stirring the emulsifier, glycerin and oil in proportion to uniformly prepare the lecithin mixture" is: 1:2:5.

Further, in a preferred mode of the present invention, the step "add adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone to the mixed solution of hydroxypropyltetrahydropyrantriol in proportion and mix well The specific addition mass ratio of the adenosine, the carnosine, the transdermal peptide and the p-hydroxyacetophenone in "Preparing the Polypeptide Mixture" is: 1:1:1:(0.1-1).

Preferably, the adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone are added in proportion to the hydroxypropyltetrahydropyrantriol mixture and mixed uniformly to prepare the polypeptide mixture. The specific mass ratio of glucoside, the carnosine, the transdermal peptide and the p-hydroxyacetophenone added is: 1:1:1:(0.2-0.8).

In another preferred manner, the step "add adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone to the mixed solution of hydroxypropyltetrahydropyrantriol in proportion and mix uniformly to prepare the mixed solution of polypeptide The specific mass ratio of the adenosine, the carnosine, the transdermal peptide and the p-hydroxyacetophenone is 1:1:1:0.5.

According to the technical solution provided by the present invention, compared with the prior art, the anti-aging transdermal polypeptide preparation related to the present invention includes the following components by weight percentage: hydroxypropyltetrahydropyrantriol 1%-50% , collagen peptide 0.1%-5%, adenosine 0.1%-1.0%, carnosine 0.1%-0.5%, transdermal peptide 0.5%-5.0%, p-hydroxyacetophenone 0.4%-0.8% and the rest of water, In this way, the anti-aging transdermal polypeptide preparation provided by this application contains a variety of anti-aging ingredients. Hydroxypropyltetrahydropyranotriol can effectively promote the regeneration of aging collagen, make the cells more plump, and stabilize the new collagen in the skin Protein and adenosine can stimulate the renewal of fibroblasts. Carnosine can effectively prevent glycosylation, inhibit lipid oxidation caused by free radicals and metal ions, and can build skin's anti-photoaging barrier. Transdermal peptide can help skin improve hydroxyl The absorption rate of propyl tetrahydropyranotriol improves the absorption rate of anti-aging ingredients in the skin, and carnosine can help the new collagen to increase the skin's resistance, maintain the activity of new cells under free radical conditions, and effectively maintain the anti-aging effect. aging effect, and, in the preparation process of the anti-aging transdermal polypeptide preparation, due to the homogenization effect of the two-stage high-pressure shear micro-jet, the hydroxypropyltetrahydropyrantriol and the squalane are in the Under the action of lecithin, small droplets with a particle size of less than 50nm are formed, which can reach the basal layer of the skin more easily and improve the absorption rate. Moreover, the anti-aging transdermal polypeptide preparation is a liposome nano-preparation, which reduces the amount of water lost during use. The possibility of being hydrolyzed improves the anti-aging effect.

Detailed ways

In order to enable those skilled in the art to better understand the technical solutions in the application, the technical solutions in the embodiments of the application will be clearly and completely described below. Obviously, the described embodiments are only part of the implementation of the application. example, not all examples. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of this application.

The present application provides an anti-aging transdermal polypeptide preparation, which includes the following components by weight percentage: 1%-50% of hydroxypropyltetrahydropyranotriol, 0.1%-5% of collagen peptide, and 0.1% of adenosine -1.0%, carnosine 0.1%-0.5%, transdermal peptide 0.5%-5.0%, p-hydroxyacetophenone 0.4%-0.8% and the balance of water.

An embodiment of the present invention provides an anti-aging transdermal polypeptide preparation, including the following components by weight percentage: 1%-50% of hydroxypropyltetrahydropyranotriol, 0.1%-5% of collagen peptide, adenosine 0.1%-1.0%, carnosine 0.1%-0.5%, transdermal peptide 0.5%-5.0%, p-hydroxyacetophenone 0.4%-0.8% and the rest of water, so the anti-aging transdermal polypeptide preparation provided by this application Contains a variety of anti-aging ingredients. Hydroxypropyltetrahydropyranotriol can effectively promote the regeneration of aging collagen, make cells more plump, and stabilize the new collagen in the skin. Adenosine can stimulate the renewal of fibroblasts, Carnosine can effectively prevent glycosylation, inhibit lipid oxidation caused by free radicals and metal ions, and build skin’s anti-photoaging barrier. Transdermal peptide can help the skin increase the absorption rate of hydroxypropyltetrahydropyranotriol, Improve the absorption rate of skin anti-aging ingredients, and carnosine can help new collagen increase skin resistance, maintain the activity of new cells under free radical conditions, and effectively maintain anti-aging effects, and, in anti-aging transdermal peptide preparations During the preparation process, due to the homogenization of two-stage high-pressure shear micro-jet, the hydroxypropyltetrahydropyrantriol and the squalane form small particles with a particle size of less than 50nm under the action of the lecithin. Droplets are easier to reach the base layer of the skin, which improves the absorption rate. Moreover, the anti-aging transdermal polypeptide preparation is a liposome nano-preparation, which reduces the possibility of being hydrolyzed during use and improves the anti-aging effect .

More specifically, in an embodiment of the present invention, the anti-aging transdermal polypeptide preparation includes the following components by weight percentage: 5%-50% of hydroxypropyltetrahydropyranotriol, 0.5% of collagen peptide %-5%, adenosine 0.5%-1.0%, carnosine 0.1%-0.5%, transdermal peptide 0.5%-5.0%, p-hydroxyacetophenone 0.4%-0.8% and the balance of water.

More specifically, in an embodiment of the present invention, the anti-aging transdermal polypeptide preparation includes the following components by weight percentage: 10%-50% of hydroxypropyltetrahydropyrantriol, 0.5% of collagen peptide %-5%, adenosine 0.6%-1.0%, carnosine 0.2%-0.5%, transdermal peptide 1.0%-5.0%, p-hydroxyacetophenone 0.4%-0.7% and the balance of water.

More specifically, in an embodiment of the present invention, the anti-aging transdermal polypeptide preparation includes the following components by weight percentage: 20%-50% of hydroxypropyl tetrahydropyrantriol, 0.5% of collagen peptide %-5%, adenosine 0.6%-1.0%, carnosine 0.2%-0.5%, transdermal peptide 1.5%-5.0%, p-hydroxyacetophenone 0.4%-0.7% and the balance of water.

What needs to be added is that in the embodiment of the present invention, the anti-aging transdermal polypeptide preparation also includes the following components by weight percentage: 1%-10% palmitoyl tripeptide-5, 1%-5% Glutathione, 1%-5% deer antler polypeptide.

It should be added that palmitoyl tripeptide-5 can inhibit the production of melanin by inhibiting the activity of tyrosinase, down-regulating the mRNA levels of MIFT and tyrosinase, and can significantly reduce the level of melanin in the supernatant and cells without Cytotoxicity, and palmitoyl tripeptide-5 can significantly reduce the gray value of melanin distribution in UV-induced skin pigmentation.

It needs to be added that the reason why glutathione can protect the skin and resist cell aging is mainly because it can remove excess free radicals and some peroxides in skin tissue, and can significantly prevent the lipids of mitochondria in cells from Peroxidation.

What needs to be added is that velvet antler polypeptides have good antioxidant and anti-inflammatory activities. velvet antler polypeptides not only play an important role in the immune system, circulatory system, nervous system, bone metabolism, and glucose metabolism, but also play an important role in inflammation, virus suppression, and anti-inflammatory effects. Oxidation and other aspects also have outstanding effects.

Specifically, in the embodiment of the present invention, the anti-aging transdermal polypeptide preparation is a liposomal nano preparation.

Specifically, in the embodiment of the present invention, the average particle size of the anti-aging transdermal polypeptide preparation is 10-60nm.

More specifically, in the embodiment of the present invention, the average particle size of the anti-aging transdermal polypeptide preparation is 10-50 nm.

The present application also provides a preparation method of an anti-aging transdermal polypeptide preparation for preparing the above-mentioned anti-aging transdermal polypeptide preparation, comprising the following steps:

Mixing hydroxypropyltetrahydropyrantriol, collagen peptide and deionized water evenly to prepare a hydroxypropyltetrahydropyrantriol mixed solution;

Mix and stir the emulsifier, glycerin and oil in proportion to obtain a lecithin mixture; add adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone to the hydroxypropyltetrahydropyranotriol in proportion and mix Mix in the liquid evenly to prepare the polypeptide mixed liquid;

The polypeptide mixture is added to the lecithin mixture, and two-stage high-pressure shear homogenization is carried out to obtain an anti-aging transdermal polypeptide preparation.

Specifically, in an embodiment of the present invention, the specific components of the hydroxypropyltetrahydropyrantriol mixture include: hydroxypropyltetrahydropyrantriol with a mass concentration of 1%-55%, the mass concentration 0.1%-5% Collagen Peptides.

More specifically, in the embodiment of the present invention, the specific components of the hydroxypropyltetrahydropyrantriol mixture include: hydroxypropyltetrahydropyrantriol with a mass concentration of 10%-55%, Collagen peptides with a mass concentration of 0.5%-5%.

Specifically, in the embodiment of the present invention, the specific conditions of the step "adding the polypeptide mixture to the lecithin mixture, and performing two-stage high-pressure shear homogenization to obtain an anti-aging transdermal polypeptide preparation" are as follows: The first-stage homogeneous pressure is 20-60Mpa, the temperature is 10-35°C, and the shear rate is 10-500r/min; the second-stage homogeneous pressure is 60-110Mpa, the temperature is 15-40°C, and the shear rate is 10-500r/min.

More specifically, in an embodiment of the present invention, the speed of the first-stage high-pressure shear is 40-500r/min, and the speed of the second-stage high-pressure shear is 60-500r/min.

More specifically, in the embodiment of the present invention, the time for the first homogenization is 5-40 minutes, and the time for the second homogenization is 10-30 minutes.

More specifically, in the embodiment of the present invention, the two-stage high-pressure shear homogenizer is a two-stage high-pressure shear micro-jet homogenizer, and the flow rate of the two-stage micro-jet is 100-500 ml/min.

More specifically, in the embodiment of the present invention, the flow rate of the first stage micro jet is 150-500ml/min, and the flow rate of the second stage micro jet is 250-500ml/min.

More specifically, in an embodiment of the present invention, the first-stage micro-jet flow rate is 200-500ml/min, and the second-stage micro-jet flow rate is 280-500ml/min.

Specifically, in the embodiment of the present invention, the emulsifier in the step of "mixing and stirring the emulsifier, glycerin and oil in proportion to uniformly prepare the lecithin mixture" is lecithin.

Specifically, in the embodiment of the present invention, the oil in the step "mixing and stirring the emulsifier, glycerin and oil in proportion to uniformly prepare the lecithin mixture" is squalane.

Specifically, in the embodiment of the present invention, the specific mass ratio of the emulsifier, the glycerin and the oil added in the step "mixing and stirring the emulsifier, glycerin and oil in proportion to uniformly prepare the lecithin mixture" is: 1:(1-4):5.

More specifically, in the embodiment of the present invention, in the step "mix and stir the emulsifier, glycerin and oil in proportion to uniformly prepare the lecithin mixture", the specific mass ratio of the emulsifier, the glycerin and the oil added is For: 1:2:5.

Specifically, in the embodiment of the present invention, the step "add adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone to the hydroxypropyltetrahydropyrantriol mixture in proportion and mix uniformly to prepare the polypeptide The specific addition mass ratio of the adenosine, the carnosine, the transdermal peptide and the p-hydroxyacetophenone in the "mixed solution" is: 1:1:1:(0.1-1).

More specifically, in the embodiment of the present invention, the step "add adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone to the mixed solution of hydroxypropyltetrahydropyrantriol in proportion and mix uniformly to prepare The specific addition mass ratio of the adenosine, the carnosine, the transdermal peptide and the p-hydroxyacetophenone in "Polypeptide Mixture" is: 1:1:1:(0.2-0.8).

More specifically, in the embodiment of the present invention, the step "add adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone to the mixed solution of hydroxypropyltetrahydropyrantriol in proportion and mix well The specific mass ratio of the adenosine, the carnosine, the transdermal peptide and the p-hydroxyacetophenone added in "Preparing the Polypeptide Mixture" is: 1:1:1:0.5.

In order to better illustrate the anti-aging effect of the anti-aging transdermal polypeptide preparation involved in the examples of the present invention, the following evaluation experiments were carried out:

1. Determination of skin quality improvement efficacy of anti-aging transdermal polypeptide preparations

Taking the anti-aging transdermal polypeptide system involved in the embodiment of the present invention and similar anti-aging products on the market as examples, a 16-week skin quality improvement test was carried out.

20 female subjects with healthy skin were selected. The left half of the testees smeared the anti-aging transdermal polypeptide preparation related to the embodiment of the present invention as the test group, and the right half of the face did not smear any substance as the control group. The test period was 16 weeks. At the 8th week and the 16th week, various instruments were used to detect and analyze the skin of the subject to evaluate the anti-aging transdermal polypeptide preparations involved in the embodiments of the present invention for skin roughness, skin gloss, skin moisture content and skin elasticity. The improvement efficacy, the average value of the two tests in the 8th week and the 16th week are shown in Table 1.

Table 1 Efficacy Test for Skin Quality Improvement

Note: "+" means increase, "-" means decrease.

2. Transdermal Performance Test of Anti-aging Transdermal Peptide Preparations

Experimental group 1

Mix hydroxypropyltetrahydropyrantriol, collagen peptide and deionized water evenly to obtain a mixture of 40% hydroxypropyltetrahydropyrantriol and 2% collagen peptide ;

Add lecithin, glycerin and squalane in a mass ratio of 1:2:5, mix and stir evenly to prepare a lecithin mixture;

Adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone are added to the hydroxypropyltetrahydropyrantriol mixed solution according to the mass ratio of 2:2:2:1 and mixed uniformly to prepare a polypeptide mixed solution;

Add the polypeptide mixture to the lecithin mixture at a mass ratio of 1:1, perform two-stage high-pressure shear microjet homogenization to obtain an anti-aging transdermal polypeptide preparation, and two-stage high-pressure shear microfluidics The specific conditions for jet homogenization are: the first stage homogenization pressure is 50Mpa, the homogenization time is 20min, the temperature is 30°C, the shear speed is 40r/min, the flow rate is 250ml/min, and the second stage homogenization pressure is 100Mpa , the homogenization time is 10-30min, the temperature is 15-40°C, the shear speed is 60r/min, and the flow rate is 300ml/min.

The prepared anti-aging transdermal polypeptide preparation was tested for in vitro transdermal penetration performance, and the results are shown in Table 2.

The transdermal performance detection of the anti-aging transdermal polypeptide preparation obtained in table 2 experimental group 1

Experimental group 2

Mix hydroxypropyl tetrahydropyrantriol, collagen peptide and deionized water evenly to obtain a mixed solution of hydroxypropyl tetrahydropyrantriol with a mass concentration of 40%, and a collagen peptide with a mass concentration of 0.5% ;

Add lecithin, glycerin and squalane in a mass ratio of 1:2:5, mix and stir evenly to prepare a lecithin mixture;

Adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone are added to the hydroxypropyltetrahydropyrantriol mixed solution according to the mass ratio of 2:2:2:1 and mixed uniformly to prepare a polypeptide mixed solution;

Add the polypeptide mixture to the lecithin mixture, add according to the mass ratio of 1:1, perform two-stage high-pressure shear microjet homogenization to obtain an anti-aging transdermal polypeptide preparation, The specific conditions for jet homogenization are: the first stage homogenization pressure is 50Mpa, the homogenization time is 20min, the temperature is 30°C, the shear speed is 40r/min, the flow rate is 250ml/min, and the second stage homogenization pressure is 100Mpa , the homogenization time is 10-30min, the temperature is 15-40°C, the shear speed is 60r/min, and the flow rate is 300ml/min.

The prepared anti-aging transdermal polypeptide preparation was tested for in vitro transdermal penetration performance, and the results are shown in Table 3.

The transdermal performance detection of the anti-aging transdermal polypeptide preparation obtained in table 3 experimental group 2

Experimental group 3

Mix hydroxypropyltetrahydropyrantriol, collagen peptide and deionized water evenly to obtain a mixed solution of hydroxypropyltetrahydropyrantriol with a mass concentration of 20%, and a collagen peptide with a mass concentration of 2%. ;

Add lecithin, glycerin and squalane in a mass ratio of 1:2:5, mix and stir evenly to prepare a lecithin mixture;

Adenosine, carnosine, transdermal peptide, and p-hydroxyacetophenone are added to the hydroxypropyltetrahydropyrantriol mixed solution according to the mass ratio of 2:2:2:1 and mixed uniformly to prepare a polypeptide mixed solution;

Add the polypeptide mixture to the lecithin mixture, add according to the mass ratio of 1:1, perform two-stage high-pressure shear microjet homogenization to obtain an anti-aging transdermal polypeptide preparation, The specific conditions for jet homogenization are: the first stage homogenization pressure is 50Mpa, the homogenization time is 20min, the temperature is 30°C, the shear speed is 40r/min, the flow rate is 250ml/min, and the second stage homogenization pressure is 100Mpa , the homogenization time is 10-30min, the temperature is 15-40°C, the shear speed is 60r/min, and the flow rate is 300ml/min.

The prepared anti-aging transdermal polypeptide preparation was tested for in vitro transdermal penetration performance, and the results are shown in Table 4.

The transdermal performance detection of anti-aging transdermal polypeptide preparation obtained in table 4 experimental group 3

Experimental group 4

Mixing hydroxypropyltetrahydropyrantriol and deionized water evenly to prepare a mixed solution of hydroxypropyltetrahydropyrantriol with a mass concentration of 20%;

Add lecithin, glycerin and squalane in a mass ratio of 1:2:5, mix and stir evenly to prepare a lecithin mixture;

Add the mixed solution of hydroxypropyltetrahydropyrantriol to the mixed solution of lecithin, add according to the mass ratio of 1:1, and stir evenly.

The prepared anti-aging transdermal polypeptide preparation was tested for in vitro transdermal penetration performance, and the results are shown in Table 5.

The transdermal performance detection of the anti-aging transdermal polypeptide preparation obtained in table 5 experimental group 4

It should be added that the test methods for each index in the above experimental group are as follows:

Set the transdermal diffusion tester at the same time with 4 diffusion cells, the area of the cell mouth is 3cm2, and the volume of each cell is 8ml. Use 0.9% normal saline as the receiving solution, and use the abdominal skin of the mouse as the permeable skin. Take 4 mice with similar body weights. , shave off the hair on the abdomen, after feeding for 24 hours, remove the subcutaneous fat, remove the miscellaneous hair, wash with normal saline, apply the same amount of anti-aging transdermal polypeptide preparation obtained in experimental group 1 to 4 mice respectively, and experimental group 2 The obtained anti-aging transdermal polypeptide preparation, the anti-aging transdermal polypeptide preparation obtained in experimental group 3, and the anti-aging transdermal polypeptide preparation obtained in experimental group 4 were applied to the diffusion cell for 20 minutes to carry out hydroxypropyltetrahydropyrantriol treatment. The transmittance test, the test results are shown in Table 6.

Table 6 Hydroxypropyl tetrahydropyranotriol transmission rate detection

From the test data in Table 1, it is found that the anti-aging transdermal polypeptide preparation provided by this application can increase skin elasticity, enhance skin gloss, effectively improve the problem of rough skin, and have a good moisturizing effect. From the test data in Table 2-Table 5 It was found that in the process of preparing anti-aging transdermal polypeptide preparations, adding collagen peptides can increase the permeability of hydroxypropyltetrahydropyranotriol. At the same time, in the process of preparing anti-aging transdermal polypeptide preparations, after After high-pressure shearing micro-jet homogeneous treatment, the transmittance of hydroxypropyl tetrahydropyrantriol can be improved, and the stability of hydroxypropyl tetrahydropyrantriol and carnosine entering the skin can be improved, and the The rate of absorption by the skin.

It should be added that the product form of the anti-aging transdermal polypeptide preparation includes face cream, mask, lotion, and essence.

The above description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention will not be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (1)

1. a preparation method of anti-aging transdermal polypeptide preparation, is characterized in that, comprises the following steps: 1%-50% by weight of hydroxypropyltetrahydropyranotriol, 0.1%-5% by weight of collagen peptide and deionized water are uniformly mixed to prepare hydroxypropyltetrahydropyranotriol mixed solution; Mix and stir the emulsifier, glycerin and oil in proportion to obtain a lecithin mixture. The specific mass ratio of the emulsifier, the glycerin and the oil is 1: (1-4): 5, and the emulsifier It is lecithin, and the oil is squalane; 0.1%-1.0% by weight of adenosine, carnosine, 0.5%-5.0% by weight of transdermal peptide, and 0.4%-0.8% by weight of p-hydroxyacetophenone are added to the hydroxy Propyltetrahydropyranotriol mixed solution is mixed uniformly to prepare polypeptide mixed solution, and the specific addition mass ratio of the adenosine, the carnosine, the transdermal peptide and the p-hydroxyacetophenone is 1:1: 1: (0.1-1); Add the polypeptide mixture to the lecithin mixture, and perform two-stage high-pressure shear homogenization to obtain an anti-aging transdermal polypeptide preparation. The specific conditions for the two-stage high-pressure shear homogenization are: the first stage The homogeneous pressure is 20-60Mpa, the temperature is 10-35°C, the shear speed is 10-500r/min, the second stage homogeneous pressure is 60-110Mpa, the temperature is 15-40°C, and the shear speed is 10-500r /min.