CN118086153B - Low-temperature denitrification phosphorus-accumulating crane feather Tian Daier Ford strain and application thereof - Google Patents
Low-temperature denitrification phosphorus-accumulating crane feather Tian Daier Ford strain and application thereof Download PDFInfo
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- 210000003746 feather Anatomy 0.000 title abstract description 31
- 241001051769 Delftia tsuruhatensis Species 0.000 claims abstract description 37
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 24
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 24
- 239000011574 phosphorus Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 238000004321 preservation Methods 0.000 claims abstract description 18
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 15
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 15
- 239000010452 phosphate Substances 0.000 claims abstract description 15
- 238000009629 microbiological culture Methods 0.000 claims abstract description 6
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 8
- 241000233866 Fungi Species 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 5
- 239000010865 sewage Substances 0.000 abstract description 5
- 229910002651 NO3 Inorganic materials 0.000 abstract description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 description 22
- 241000894006 Bacteria Species 0.000 description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 230000001580 bacterial effect Effects 0.000 description 14
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 11
- 239000001110 calcium chloride Substances 0.000 description 11
- 229910001628 calcium chloride Inorganic materials 0.000 description 11
- 239000012153 distilled water Substances 0.000 description 11
- 239000007788 liquid Substances 0.000 description 10
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 235000013619 trace mineral Nutrition 0.000 description 8
- 239000011573 trace mineral Substances 0.000 description 8
- 229910052564 epsomite Inorganic materials 0.000 description 7
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 238000012216 screening Methods 0.000 description 7
- 239000010802 sludge Substances 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 6
- 239000007836 KH2PO4 Substances 0.000 description 6
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 5
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 4
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 4
- 229910052927 chalcanthite Inorganic materials 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 239000011565 manganese chloride Substances 0.000 description 4
- 229910052603 melanterite Inorganic materials 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 4
- 229910000368 zinc sulfate Inorganic materials 0.000 description 4
- 239000011686 zinc sulphate Substances 0.000 description 4
- 241001052560 Thallis Species 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 241000186000 Bifidobacterium Species 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
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- C02F2101/163—Nitrates
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
A low-temperature denitrification phosphorus-accumulating crane Feng Tian Daier Ford strain and application thereof belong to the technical field of environmental biology. The invention aims to screen out a strain which has strong adaptability under the low temperature condition and has denitrification phosphorus accumulating capacity, thereby improving the denitrification and phosphorus removing capacity of a sewage treatment plant under the northern cold condition. The Crane feather Tian Daier Ford fungus (Delftia tsuruhatensis) TNP-1 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation address of No. 3 of Seway 1, no. 3 of the Korean area North Star of Beijing, a preservation date of 2024, 01 month and 22 days, and a preservation number of CGMCC No.29715. A low-temperature denitrification phosphorus accumulating Crane-feather Tian Daier Ford strain is used for low-temperature denitrification phosphorus accumulating, the removal rate of nitrate in water can reach 83.14% at 10 ℃, and the removal rate of phosphate in water can reach 71.26% at 10 ℃.
Description
Technical Field
The invention belongs to the technical field of environmental biology, and relates to a crane feather Tian Daier Ford strain and application thereof.
Background
Compared with the traditional denitrification and dephosphorization bacteria, the discovery of the aerobic denitrification and dephosphorization bacteria provides possibility for solving the contradiction between carbon source utilization and sludge age difference, so that denitrification and dephosphorization can be synchronously carried out, however, in northern cold areas, after entering winter, the denitrification and dephosphorization capability of microorganisms is often inhibited due to low water temperature, and the problem that the discharge of TP or TN of a sewage treatment plant is not up to standard is highlighted, so that the problem that the removal of nitrogen and phosphorus is incomplete is remarkably solved, and therefore, the screening of a strain which has stronger adaptability and denitrification and dephosphorization capability under the low-temperature condition is very necessary for improving the pollutant treatment efficiency of sewage plants in the cold areas.
Disclosure of Invention
The invention aims to screen out a strain which has strong adaptability and denitrification phosphorus accumulating capacity under the low-temperature condition, thereby improving the denitrification and dephosphorization capacity of a sewage treatment plant under the northern cold condition, and provides a low-temperature denitrification phosphorus accumulating crane feather Tian Daier Ford strain and application thereof.
The low-temperature denitrification phosphorus-accumulating Crane Feng Tian Daier Ford strain is crane Feng Tian Daier Ford (Delftia tsuruhatensis) TNP-1, and is preserved in China general microbiological culture Collection center (China Committee for culture Collection), the preservation address is North Star Xiyu No. 1, 3 in the Korean region of Beijing, the preservation date is 2024, 01 and 22 days, and the preservation number is CGMCC No. 29715.
A low-temperature denitrification phosphorus accumulating Crane-feather Tian Daier Ford strain is used for low-temperature denitrification phosphorus accumulating; the low temperature is 10 ℃.
A low-temperature denitrification phosphorus-accumulating Crane-feather Tian Daier Ford strain is used for removing nitrate nitrogen and phosphate in water under a low-temperature condition.
The Crane feather Tian Daier Ford (Delftia tsuruhatensis) TNP-1 has the following properties:
The Crane feather Tian Daier Ford (Delftia tsuruhatensis) TNP-1 is inoculated on LB culture medium, and after culturing for 72 hours at 10 ℃, single colony is round, smooth and moist in surface, almost white and opaque. Single colonies were sequenced using 16S rRNA gene Sanger sequencing, strain gene sequences were aligned to NCBI database by BLAST, and the result showed that the closest homology to strain TNP-1 was crane feather Tian Daier ford (Delftia tsuruhatensis) CM13, and finally strain TNP-1 was determined to be crane feather Tian Daier ford (Delftia tsuruhatensis), and the sequences were uploaded to Genbank database to obtain accession numbers: PP425222.
The invention has the beneficial effects that:
1. The nitrate in water is removed by adopting the crane feather Tian Daier Ford (Delftia tsuruhatensis) TNP-1 at 10 ℃, and the removal rate can reach 83.14%;
2. The crane feather Tian Daier Ford (Delftia tsuruhatensis) TNP-1 is adopted to remove phosphate in water at 10 ℃, and the removal rate can reach 71.26%;
the invention also provides a separation and screening method of the Crane feather Tian Daier Ford (Delftia tsuruhatensis) TNP-1, which comprises the following steps: the aerobic granular sludge in the low-temperature reactor is subjected to enrichment, domestication, culture, separation, purification and step-by-step screening, so that the aerobic granular sludge can be obtained, and the denitrification and dephosphorization capability of a sewage treatment plant under the northern cold condition is improved.
The aerobic granular sludge is sourced from an SBR reactor which is stably operated under the low-temperature condition of 10 ℃.
Drawings
FIG. 1 is a diagram showing growth of solid medium of Crane-feather Tian Daier Ford (Delftia tsuruhatensis) TNP-1;
FIG. 2 is a graph showing the effect of TNP-1 of Crane Bifidobacterium Tian Daier (Delftia tsuruhatensis) on removal of nitrate nitrogen at 10deg.C in example 2;
FIG. 3 is a graph showing the effect of TNP-1 of the Crane-feather Tian Daier Ford (Delftia tsuruhatensis) of example 3 on removal of phosphate at 10 ℃;
The Crane feather Tian Daier Ford fungus (Delftia tsuruhatensis) TNP-1 is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation address of No. 3 of Seway 1 of the Korean area North Star of Beijing, a preservation date of 2024, 01 month and 22 days and a preservation number of CGMCC No. 29715.
Detailed Description
The following examples are used to verify the benefits of the present invention:
Example 1: screening and identifying the Crane feather Tian Daier Ford fungus (Delftia tsuruhatensis) TNP-1 comprises the following steps:
(one), enrichment and screening: mature granular sludge is obtained from a low-temperature aerobic granular sludge SBR reactor for laboratory self-culture, the granular sludge is inoculated into an enrichment and domestication culture medium according to the inoculum size of 10 percent, a constant-temperature shaking table is used for culturing at 10 ℃ and 120rpm, after repeated inoculation and culture are carried out for 5 times, enriched bacterial liquid is subjected to gradient dilution, diluent with the dilution factor of 10 -5 is selected and coated into a solid culture medium, the culture is carried out at 10 ℃ in an inverted mode, single bacterial colony is selected and placed into a BTB culture medium for blue and white spot screening, the bacterial colony with blue indication marks appearing around is subjected to different-dyeing particle dyeing, the bacterial colony with black particles is inoculated into a nitrogen-enriched and phosphorus-enriched culture medium, the bacterial colony with the denitrification and phosphorus-accumulating capacity is cultivated at 10 ℃ and 120rpm, and single bacterial colony is subjected to 3 times of streak purification, so that pure bacteria are obtained, numbered and preserved.
The culture medium sterilization conditions are as follows:
enrichment domestication culture medium ：CH3COONa·3H2O 3.32g/L,KH2PO4 0.04g/L,NH4Cl 0.305 g/L,KNO3 0.3g/L,MgSO4·7H2O 0.091g/L,CaCl2·2H2O 0.026g/L,NaCl 0.02g/L, microelement 2mL, distilled water 1000mL,pH 7.2~7.4, sterilization at 121 ℃ 30 min;
BTB medium ：C4H4Na2O4 8.5 g/L,KNO3 1.0 g/L,KH2PO4 1.0 g/L,FeCl3·6H2O MgSO4·7H2O 1.0g/L,CaCl2·7H2O 0.2g/L,BTB alcoholic solution (1 g BTB in 1L of 20% mass fraction alcohol) 1ml, ph 7.2, sterilized at 121 ℃ for 30: 30min;
2mL of nitrogen-rich and phosphorus-rich culture medium ：CH3COONa·3H2O 3.32g/L,KH2PO4 0.035g/L,NH4Cl 0.305 g/L,KNO3 0.3g/L,MgSO4·7H2O 0.091g/L,CaCl2·2H2O 0.026g/L,NaCl 0.02g/L,, 1000mL of distilled water and pH 7.2, and sterilizing at 121 ℃ for 30min;
2mL of solid culture medium ：CH3COONa·3H2O 3.32g/L,KH2PO4 0.035g/L,NH4Cl 0.305g/L,KNO30.3g/L,MgSO4·7H2O 0.091g/L,CaCl2·2H2O 0.026g/L,NaCl 0.02g/L, trace elements, 1000mL of distilled water, 20g/L of agar powder and 1000mL,pH 7.2~7.4 of distilled water, and sterilizing at 121 ℃ for 30 min.
The trace element component ：EDTA 50g/L,ZnSO4 2.2g/L,FeSO4·7H2O 5.0 g/L,MnCl2·4H2O 5.06g/L,CaCl2 5.5g/L,CoCl2·6H2O 1.61g/L,CuSO4·5H2O 1.57 g/L, in the culture medium is 1000mL of distilled water.
(II) identification: the white strain obtained by screening is sent to a biological engineering (Shanghai) stock company for sequencing, and the sequencing result is compared in a Genbank database to determine the white strain as Crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis), and the results are shown in table 1;
TABLE 1
Example 2: the nitrate nitrogen was removed at low temperature using the crane feather Tian Daier Ford (Delftia tsuruhatensis) TNP-1 selected in example 1, specifically by the following steps:
1. Adding a crane feather Tian Daier Ford (Delftia tsuruhatensis) TNP-1 bacterial liquid into the nitrogen-rich and phosphorus-rich culture medium to obtain a mixed culture;
The inoculation amount of the TNP-1 bacterial liquid of the crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) in the first step is 5%;
The content of the Crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 in the crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 in the TNP-1 bacterial liquid in the first step is 1.0x10 8 CFU/mL;
the concentration of nitrate nitrogen in the nitrogen-rich and phosphorus-rich culture medium in the first step is 15mg/L;
the Crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 is preserved in the China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation address of No. 3 of the West-road 1 of the Korean area North Star of Beijing, a preservation date of 2024, a month of 01 and a preservation number of CGMCC No. 29715;
The formula of the nitrogen-rich and phosphorus-rich culture medium in the first step is CH3COONa·3H2O 3.32g/L,KH2PO40.035g/L,NH4Cl 0.305g/L,KNO3 0.3g/L,MgSO4·7H2O 0.091g/L,CaCl2·2H2O 0.026g/L,NaCl 0.02g/L, trace elements 2mL, distilled water 1000mL, pH 7.2, and sterilizing for 30min at 121 ℃;
The trace element component ：EDTA 50g/L,ZnSO4 2.2g/L,FeSO4·7H2O 5.0 g/L,MnCl2·4H2O 5.06g/L,CaCl2 5.5g/L,CoCl2·6H2O 1.61g/L,CuSO4·5H2O 1.57 g/L, in the culture medium is 1000mL of distilled water;
2. the mixed culture was put in a shaker and centrifuged at 4℃and 5000rpm/min for 5min after 8d of culture at 10℃to complete the removal of nitrate nitrogen.
FIG. 2 is a graph showing the effect of TNP-1 of Crane Bifidobacterium Tian Daier (Delftia tsuruhatensis) on removal of nitrate nitrogen at 10deg.C in example 2;
Example 3: phosphate in the water body is removed by utilizing the crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 screened in the example 1 under the low temperature condition, and the method is specifically completed by the following steps:
1. adding TNP-1 bacterial liquid of Crane-feather Tian Daier Ford (Delftia tsuruhatensis) into a phosphorus-deficient culture medium to obtain a mixed culture I;
The inoculation amount of the Crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 in the first step is 5%;
In the first step, the content of the Crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 in the crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 in the bacterial liquid is 1.0X10 8 CFU/mL;
the Crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 is preserved in the China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation address of No. 3 of the West-road 1 of the Korean area North Star of Beijing, a preservation date of 2024, a month of 01 and a preservation number of CGMCC No. 29715;
The formula of the phosphorus-deficient culture medium in the first step is ：CH3COONa·3H2O 3.32g/L,Na2HPO4·2H2O 0.023g/L,MgSO4·7H2O 0.081g/L,K2SO4 0.018g/L,NH4Cl 0.153g/L,CaCl2·2H2O 0.011g/L, trace elements 2mL, 1000mL of distilled water, pH 7.2, and sterilizing for 30min at 121 ℃;
The trace element component ：EDTA 50g/L,ZnSO4 2.2g/L,FeSO4·7H2O 5.0 g/L,MnCl2·4H2O 5.06g/L,CaCl2 5.5g/L,CoCl2·6H2O 1.61g/L,CuSO4·5H2O 1.57 g/L, in the culture medium is 1000mL of distilled water;
2. firstly, putting the mixed culture I into a shaking table, culturing for 8 days at 10 ℃ and centrifuging for 2 min at 4 ℃ and 10000 rpm/min, and washing thalli with sterile water for 3 times to thoroughly release phosphate in bacteria;
In the second step, after the thalli are washed, the thalli need to be resuspended to the original volume so as to obtain crane feather Tian Daier Ford fungus (Delftia tsuruhatensis) TNP-1 fungus liquid after releasing phosphate;
3. adding TNP-1 bacterial liquid of Crane-feather Tian Daier Ford (Delftia tsuruhatensis) which thoroughly releases phosphate into a phosphorus-rich culture medium to obtain a mixed culture II;
The inoculation amount of the TNP-1 bacterial liquid of the crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) which thoroughly releases phosphate in the step three is 5%;
The content of the Crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 in the crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 in the crane feather Tian Daier Ford bacteria (Delftia tsuruhatensis) TNP-1 bacterial liquid which thoroughly releases phosphate in the step three is 1.0X10 8 CFU/mL;
the concentration of phosphate in the phosphorus-rich culture medium in the step three is 30mg/L;
the formula of the phosphorus-rich culture medium in the third step is CH3COONa· 3H2O 3.32g/L,KH2PO4 0.04 g/L,NH4Cl 0.305g/L, MgSO4·7H2O 0.091g/L,CaCl2·2H2O 0.026g/L, trace elements 2mL, distilled water 1000mL,pH 7.2~7.4, and sterilization is carried out at 121 ℃ for 30 min;
The trace element component ：EDTA 50g/L,ZnSO4 2.2g/L,FeSO4·7H2O 5.0 g/L,MnCl2·4H2O 5.06g/L,CaCl2 5.5g/L,CoCl2·6H2O 1.61g/L,CuSO4·5H2O 1.57 g/L, in the culture medium is 1000mL of distilled water;
4. the mixed culture II was placed in a shaker and centrifuged at 4℃and 5000rpm/min for 5min after 8 d incubation at 10℃to complete phosphate removal.
FIG. 3 is a graph showing the effect of TNP-1 of the Crane-feather Tian Daier Ford (Delftia tsuruhatensis) of example 3 on removal of phosphate at 10 ℃;
from FIGS. 2 and 3, it can be seen that the removal rate of nitrate nitrogen in the nitrogen-rich and phosphorus-rich culture medium of Crane-type Tian Daier Ford (Delftia tsuruhatensis) TNP-1 can reach 83.14% and the removal rate of phosphate can reach 71.26% at a low temperature of 10 ℃.
Claims (3)
1. A low-temperature denitrification phosphorus-accumulating Crane Feng Tian Daier Ford strain is characterized in that the strain is crane Feng Tian Daier Ford (Delftia tsuruhatensis) TNP-1, and is preserved in China general microbiological culture Collection center (China Committee for culture Collection), the preservation address is North Star Xiya No.1, no. 3, the preservation date is 2024, the year 01 and the month 22, and the preservation number is CGMCC No. 29715.
2. The use of a strain of low temperature denitrifying phosphorus accumulating crane plume Tian Daier ford as claimed in claim 1, characterized in that the strain of low temperature denitrifying phosphorus accumulating crane plume Tian Daier ford is used for low temperature denitrifying phosphorus accumulating; the low temperature is 10 ℃.
3. Use of a strain of low temperature denitrifying phosphorus accumulating crane plume Tian Daier ford according to claim 2, characterized in that it is used for removing nitrate nitrogen and phosphate from water under low temperature conditions.
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| CN101016525A (en) * | 2006-10-13 | 2007-08-15 | 北京工商大学 | Delftia with aerobic denitrifying capability and method of treating waste water by the same |
| CN105861359A (en) * | 2016-05-17 | 2016-08-17 | 中国石油大学(华东) | Heterotrophic nitrification-aerobic denitrification high temperature resisting strain for producing floc, and application thereof |
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| CN105861359A (en) * | 2016-05-17 | 2016-08-17 | 中国石油大学(华东) | Heterotrophic nitrification-aerobic denitrification high temperature resisting strain for producing floc, and application thereof |
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