CN103783052B - A kind of novel mouse sterile bait and preparation method thereof - Google Patents

Abstract

The invention belongs to field of pharmacology, be specifically related to a kind of novel mouse sterile bait and preparation method thereof.The present invention adopts quinestrol and anticoagulant as active ingredient, and composition is simple, has both been applicable to male mouse, and has also been applicable to female mouse.In sterile bait, add anticoagulant, not only can deratize further, sterile bait can also be avoided the unconspicuous defect of some mouse effect.And quinestrol can reduce the toxicity of anticoagulant further, make anticoagulant longer for action time in mouse body, solve the problem of mouse food refusal further.Meanwhile, the present invention, by adding oleandrin as active ingredient, facilitates quinestrol and anticoagulant greatly to the action effect of mouse.By adopting the present invention, sterile and dead mouse is more than 97% of total number, and action effect is obvious.

Description

A novel rodent sterile bait and its preparation method

technical field

The invention belongs to the field of pharmacy, and in particular relates to a novel rodent sterile bait and a preparation method thereof.

Background technique

Rodents are the most diverse, most widely distributed, and most numerous group of mammals. The amount of rodenticide is small, and mice will not have food refusal to poison bait; second, it is in line with the habit of mice stealing food. Rats often eat on and off, intermittently, and most chronic rodenticides are not very toxic to rats once eaten, and the rats will not feel uncomfortable after eating, and they can continue to steal food for several days until the poison accumulates to a certain amount , mice will be poisoned to death. Acute rodenticides are in contrast to this. Rats are prone to food refusal. At the same time, due to the rapid action of acute rodenticides, some mice that have eaten the poison bait but have not reached the lethal dose will feel uncomfortable and will not eat it for the second time. Will eat again and be able to continue to survive. Therefore, the effect of acute rodenticide is poor, generally only 50% to 60% of the control effect.

Anticoagulant rodenticides are the most commonly used chronic rodenticides. Generally, the method of low concentration and multiple delivery is adopted. However, with the long-term and extensive use of anticoagulant agents to kill rodents, the problem of rodent resistance has become more and more prominent, which has seriously plagued the development of rodent prevention work.

At present, countries all over the world are devoting themselves to the research of new technologies for sustainable control of rodent pests, especially sterile agent technology. Sterility control refers to the use of reproductive biology and ecological techniques and methods to sterilize males or females, or to block the growth and development of larvae, so as to reduce the fertility rate and control the population density of harmful organisms. Compared with traditional chemical poisoning, sterilizers are safer, have less environmental pollution, less harm to non-target organisms, are easily accepted by the public, and have a longer-lasting effect on sterility control. However, only carrying out sterility control on mice cannot maximize the prevention and control of rodent damage. Sterile mice still bring certain economic losses to people. And the sterile bait has no obvious effect on some mice.

Contents of the invention

The purpose of the present invention is to provide a novel rodent sterile bait and its preparation method. It can not only reduce the birth rate of rodents, control the population density of harmful organisms, but also further eliminate rats, effectively avoiding the defect that sterile bait has no obvious effect on some rats.

The technical solution adopted in the present invention is a novel rodent sterile bait, the bait is composed of fish meal, silkworm chrysalis, blood meal, corn, soybean oil, soybean, sodium chloride, anticoagulant, ethinyl estradiol, and eucalyptus Composition of peach glycoside C, the parts by weight of each component in each bait are 8~12 parts of fish meal, 8~12 parts of silkworm chrysalis, 8~12 parts of blood meal, 440~460 parts of corn, 55~65 parts of soybean oil, 440 parts of soybean ~460 parts, 8~12 parts of sodium chloride, 0.5~1 part of anticoagulant, 1.5~3.5 parts of ethinylestradiol, 0.1~0.5 part of oleandrin C; the anticoagulant is heparin, B One of diamine tetraacetate and sodium citrate.

A preparation method of a novel rodent sterile bait, comprising the following steps:

Step 1: weighing each component according to the parts by weight of each component;

Step 2: Ultrafine grinding of soybeans and corn at low temperature, the particle size of the ground particles is 0.5-1 μm, stirring the ground soybeans and corn evenly to obtain mixture I, and set aside;

Step 3: First add fish meal, silkworm chrysalis, blood meal, sodium chloride, ethinyl estradiol and oleandrin C to mixture I, then add an equal volume of water to it, then add anticoagulant while stirring, and stir Mixture II was obtained after homogenization;

Step 4: Put the mixture II in a granulator for processing, and obtain sterile bait with a particle size of 1-3 mm after processing, and then dry and seal the sterile bait for storage.

The beneficial effects of the present invention are:

The present invention adopts ethinyl estradiol and anticoagulant as effective ingredients, and the ingredients are simple, and is suitable for both male and female rats. Ethinyl estradiol inhibits growth hormone by interfering with the reproductive axis hypothalamus-pituitary-testis (ovary) Secretion, promote germ cell apoptosis, inhibit cell proliferation, induce reproductive organ atrophy, reduce mature sperm and egg cells, cause sterility, and have a long-lasting effect. Adding an anticoagulant to the sterile bait can not only further eliminate mice, but also avoid the defect that the sterile bait has no obvious effect on some mice. Moreover, ethinyl estradiol can further reduce the toxicity of the anticoagulant, make the anticoagulant act longer in the rat body, and further solve the problem of the rat's refusal to eat. At the same time, the present invention greatly promotes the effects of ethinylestradiol and anticoagulants on mice by adding oleandrin as an active ingredient. By adopting the present invention, the total number of sterile and dead mice is more than 97%, and the effect is obvious.

Detailed ways

The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

A new type of sterile rodent bait, the bait is composed of fish meal, silkworm chrysalis, blood meal, corn, soybean oil, soybean, sodium chloride, anticoagulant, ethinyl estradiol and oleandrin C, each bait contains The parts by weight of each component are fish meal 8~12 parts, silkworm chrysalis 8~12 parts, blood meal 8~12 parts, corn 440~460 parts, soybean oil 55~65 parts, soybean 440~460 parts, sodium chloride 8~ 12 parts, 0.5~1 part of anticoagulant, 1.5~3.5 parts of ethinylestradiol, 0.1~0.5 part of oleandrin C; the anticoagulant is heparin, ethylenediaminetetraacetic acid salt, citric acid One of the sodium.

A preparation method of a novel rodent sterile bait, comprising the following steps:

Step 1: taking each component according to the parts by weight of each component described in claim 1;

Step 2: Ultrafine grinding of soybeans and corn at low temperature, the particle size of the ground particles is 0.5-1 μm, stirring the ground soybeans and corn evenly to obtain mixture I, and set aside;

Step 3: First add fish meal, silkworm chrysalis, blood meal, sodium chloride, ethinyl estradiol and oleandrin C to mixture I, then add an equal volume of water to it, then add anticoagulant while stirring, and stir Mixture II was obtained after homogenization;

Step 4: Put the mixture II in a granulator for processing, and obtain sterile bait with a particle size of 1-3 mm after processing, and then dry and seal the sterile bait for storage.

Example 1

A new type of sterile rodent bait, the bait is composed of fish meal, silkworm chrysalis, blood meal, corn, soybean oil, soybean, sodium chloride, anticoagulant, ethinyl estradiol and oleandrin C, each bait contains The parts by weight of each component are 8 parts of fish meal, 8 parts of silkworm chrysalis, 8 parts of blood meal, 440 parts of corn, 55 parts of soybean oil, 440 parts of soybean, 8 parts of sodium chloride, 0.5 part of anticoagulant, and 1.5 parts of ethinylestradiol. part, 0.1 part of oleandrin C; the anticoagulant is one of heparin, edetate, and sodium citrate.

A preparation method of a novel rodent sterile bait, comprising the following steps:

Step 1: taking each component according to the parts by weight of each component described in claim 1;

Step 2: Carrying out low-temperature ultrafine grinding of soybeans and corn, the particle size of which is 0.5 μm after grinding, stirring the ground soybeans and corn evenly to obtain mixture I, which is set aside;

Step 3: First add fish meal, silkworm chrysalis, blood meal, sodium chloride, ethinyl estradiol and oleandrin C to mixture I, then add an equal volume of water to it, then add anticoagulant while stirring, and stir Mixture II was obtained after homogenization;

Step 4: Put the mixture II in a granulator for processing, and obtain sterile bait with a particle size of 1 mm after processing, and then dry and seal the sterile bait for storage.

Example 2

A new type of sterile rodent bait, the bait is composed of fish meal, silkworm chrysalis, blood meal, corn, soybean oil, soybean, sodium chloride, anticoagulant, ethinyl estradiol and oleandrin C, each bait contains The parts by weight of each component are 12 parts of fish meal, 12 parts of silkworm chrysalis, 12 parts of blood meal, 460 parts of corn, 65 parts of soybean oil, 460 parts of soybean, 12 parts of sodium chloride, 1 part of anticoagulant, and 3.5 parts of ethinylestradiol. part, 0.5 part of oleandrin C; the anticoagulant is one of heparin, edetate, and sodium citrate.

A preparation method of a novel rodent sterile bait, comprising the following steps:

Step 1: taking each component according to the parts by weight of each component described in claim 1;

Step 2: Low-temperature ultrafine grinding of soybeans and corn, the particle size of the ground particles is 1 μm, stirring the ground soybeans and corn evenly to obtain mixture I, and set aside;

Step 3: First add fish meal, silkworm chrysalis, blood meal, sodium chloride, ethinyl estradiol and oleandrin C to mixture I, then add an equal volume of water to it, then add anticoagulant while stirring, and stir Mixture II was obtained after homogenization;

Step 4: Put the mixture II in a granulator for processing to obtain sterile bait with a particle size of 3 mm, then dry the sterile bait and seal it for storage.

Example 3

A new type of sterile rodent bait, the bait is composed of fish meal, silkworm chrysalis, blood meal, corn, soybean oil, soybean, sodium chloride, anticoagulant, ethinyl estradiol and oleandrin C, each bait contains The parts by weight of each component are 10 parts of fish meal, 10 parts of silkworm chrysalis, 10 parts of blood meal, 450 parts of corn, 60 parts of soybean oil, 450 parts of soybean, 10 parts of sodium chloride, 0.75 parts of anticoagulant, and 2.5 parts of ethinyl estradiol. part, 0.3 part of oleandrin C; the anticoagulant is one of heparin, edetate, and sodium citrate.

A preparation method of a novel rodent sterile bait, comprising the following steps:

Step 1: taking each component according to the parts by weight of each component described in claim 1;

Step 2: Ultrafine grinding of soybeans and corn at low temperature, the particle size of the ground particles is 0.75 μm, stirring the ground soybeans and corn evenly to obtain mixture I, and set aside;

Step 3: First add fish meal, silkworm chrysalis, blood meal, sodium chloride, ethinyl estradiol and oleandrin C to mixture I, then add an equal volume of water to it, then add anticoagulant while stirring, and stir Mixture II was obtained after homogenization;

Step 4: Put the mixture II in a granulator for processing to obtain sterile bait with a particle size of 2 mm, then dry the sterile bait and seal it for storage.

comparative example

The sterile bait was prepared by the above method, the difference being that oleandrin C was not added in the third step.

Test detection

Step 1: Select 400 healthy mice and divide them into four groups A, B, C and D, with 100 mice in each group;

Step 2: Prepare sterile bait according to Example 1 and Example 2 respectively, Group A is fed the sterile bait prepared in Example 1, Group B is fed the sterile bait prepared in Example 2, and Group C is fed the sterile bait prepared in Example 3. Breeding bait, group D fed the sterile bait prepared after removing oleandrin C in the present invention.

Step 3: Record the growth status of the mice every day.

The test results showed that after 10 days in group A, a total of 99 mice were infertile and died; in group B, a total of 98 mice were infertile and died after 10 days; in group C, a total of 97 mice were infertile and died after 10 days. Only, after 10 days in group B, a total of 85 mice were infertile and died. In summary, the present invention is an effective method for preventing and controlling rodent damage.

Claims (1)

1. a novel mouse sterile bait, it is characterized in that: described bait is made up of fish meal, silkworm chrysalis, blood meal, corn, soya-bean oil, soya bean, sodium chloride, anticoagulant, quinestrol and oleandrin, each composition weight number contained in every portion of bait is respectively fish meal 8 ~ 12 parts, silkworm chrysalis 8 ~ 12 parts, blood meal 8 ~ 12 parts, corn 440 ~ 460 parts, 55 ~ 65 parts, soya-bean oil, soya bean 440 ~ 460 parts, 8 ~ 12 parts, sodium chloride, anticoagulant 0.5 ~ 1 part, quinestrol 1.5 ~ 3.5 parts, oleandrin 0.1 ~ 0.5 part; Described anticoagulant is the one in heparin, edetate, sodium citrate.