CN103700096A - Method and system for analyzing images of SPR (Surface Plasmon Resonance) analyzer - Google Patents
Method and system for analyzing images of SPR (Surface Plasmon Resonance) analyzer Download PDFInfo
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Abstract
The invention discloses a method and a system for analyzing images of an SPR (Surface Plasmon Resonance) analyzer. The method comprises the steps: obtaining a first image of a biochip with a plurality of sample reaction regions to be tested, wherein the first image is the image of the biochip to which objects to be tested do not lead; obtaining a second image of the biochip after the objects to be led and tested are in reaction with a probe on the biochip; determining the position of every sample reaction region to be tested, of the biochip; dividing the first image and the second image into a plurality of local reaction images respectively according to the positions; obtaining the information of the brightness variations of the local reaction images in the same position by comparing the second image with the first image for the local reaction images in the same position; performing SPR analysis on the objects to be tested according to the information of the brightness variations. By using the method or the system, the objects to be tested, of a plurality of sample reaction regions to be tested on the biochip with a plurality of sample reaction region to be tested can be analyzed.
Description
Technical field
The present invention relates to field of biological molecule, particularly relate to a kind of image analysis method and system of SPR analyser.
Background technology
Surface plasma resonance (Surface Plasmon Resonances, SPR) sensing technology is a kind of biomolecule detection technology growing up the nineties in 20th century.Its principle is: when light is during at prism and metallic film surface generation total reflection phenomenon, can in metal film, produce disappearance ripple, when disappearance ripple occurs to resonate with surface plasma-wave, the catoptrical intensity detecting can weaken greatly.Energy is transferred to surface plasma from photon, and most of energy of incident light is absorbed by surface plasma-wave, thereby catoptrical energy is sharply reduced.Now corresponding incident angle is resonance angle (being SPR angle).SPR angle changes with the variation of metallic film surface refractive index, and the variation of refractive index is because the molecular mass of gold surface combination is directly proportional.
Concrete, SPR analyser in use, first the biochip surface that is coated with metal film fixedly one deck biomolecule identify film, then testing sample is flow through to biochip surface, if have in sample, can identify the interactional molecule of film (also claiming probe) with the biomolecule of biochip surface, can cause metallic film surface variations in refractive index, finally cause SPR angle to change, by detecting SPR angle, change, obtain the information such as concentration, affinity, kinetic constant and specificity of analyte.
But the image analysis method of SPR analyser of the prior art, is to analyze for having a testing sample conversion zone on biochip.Along with the development of technology, developed at present the biochip with a plurality of testing sample conversion zones.Use method of the prior art, cannot analyze a plurality of testing sample conversion zones on biochip.
Summary of the invention
The image analysis method and the system that the object of this invention is to provide a kind of SPR analyser, can analyze having the determinand of a plurality of testing sample conversion zones on the biochip of a plurality of testing sample conversion zones.
For achieving the above object, the invention provides following scheme:
An image analysis method for SPR analyser, comprising:
Obtain the first image of the biochip with a plurality of testing sample conversion zones; Described the first image is not for passing into the image of the biochip of determinand;
Wait passing into determinand after probe on described biochip reacts, obtain the second image of described biochip;
Determine the position of each testing sample conversion zone of described biochip;
According to described position, respectively described the first image and described the second image are divided into a plurality of local reaction images;
For the local reaction image of same position, by described the second image and described the first image, compare, obtain the brightness change information of the local reaction image of same position;
According to described brightness change information, the local reaction image of this position is carried out to SPR analysis.
Optionally, described in obtain the first image of the biochip with a plurality of testing sample conversion zones, comprising:
Employing hardware sensor pixel is the first image that more than 1,300,000 cameras obtains the biochip with a plurality of testing sample conversion zones;
Described the second image that obtains described biochip, comprising:
Employing hardware sensor pixel is the second image that more than 1,300,000 cameras obtains described biochip.
Optionally, the described position of determining each testing sample conversion zone of described biochip, comprising:
The positioning mark of the image that the described biochip of identification shooting obtains; Described positioning mark is that at least two characteristic areas on described biochip form;
According to described positioning mark, determine the size of described image;
Size according to default conversion zone size and described image, is divided into a plurality of regions by described image, and each region is corresponding with the position of a testing sample conversion zone.
Optionally, the described position of determining each testing sample conversion zone of described biochip, comprising:
Obtain the brightness value of each pixel in described the first image;
Obtain the brightness value of each pixel in described the second image;
The pixel that brightness value is changed over the first predetermined threshold value is defined as the conversion zone pixel that testing sample conversion zone is corresponding;
By the pixel set of adjacent conversion zone pixel formation, be defined as the position of testing sample conversion zone.
Optionally, the described position of determining each testing sample conversion zone of described biochip, comprising:
Obtain the brightness value of each pixel in described the first image;
Obtain the brightness value of each pixel in described the second image;
The pixel that brightness value is changed over the second predetermined threshold value is defined as center pixel corresponding to testing sample conversion zone center;
Judge that whether described center pixel exists brightness value to change the edge pixel that surpasses the 3rd predetermined threshold value, obtains the first judged result around; Described the 3rd predetermined threshold value is less than described the second predetermined threshold value;
Judgment result is that while being that the pixel set that described center pixel and described edge pixel are formed is defined as the position of testing sample conversion zone when described first.
An image analysis system for SPR analyser, comprising:
The first image acquisition unit, for obtaining the first image of the biochip with a plurality of testing sample conversion zones; Described the first image is not for passing into the image of the biochip of determinand;
The second image acquisition unit, for wait passing into determinand after probe on described biochip reacts, obtains the second image of described biochip;
Position determination unit, for determining the position of each testing sample conversion zone of described biochip;
Image cutting unit, for being divided into a plurality of local reaction images by described the first image and described the second image respectively according to described position;
Comparing unit, for the local reaction image for same position, by described the second image and described the first image are compared, obtains the brightness change information of the local reaction image of same position;
Analytic unit, for according to described brightness change information, carries out SPR analysis to the local reaction image of this position.
Optionally, described the first image acquisition unit, comprising:
The first Image Acquisition subelement is the first image that more than 1,300,000 camera obtains the biochip with a plurality of testing sample conversion zones for adopting hardware sensor pixel;
Described the second image acquisition unit, comprising:
The second Image Acquisition subelement is the second image that more than 1,300,000 camera obtains described biochip for adopting hardware sensor pixel.
Optionally, described position determination unit, comprising:
Positioning mark recognin unit, for identifying the positioning mark of taking the image that described biochip obtains; Described positioning mark is that at least two characteristic areas on described biochip form;
Size is determined subelement, for determine the size of described image according to described positioning mark;
Region Segmentation subelement, for according to the size of default conversion zone size and described image, is divided into a plurality of regions by described image, and each region is corresponding with the position of a testing sample conversion zone.
Optionally, described position determination unit, comprising:
The first brightness value obtains subelement, for obtaining the brightness value of described first each pixel of image;
The second brightness value obtains subelement, for obtaining the brightness value of described second each pixel of image;
Conversion zone pixel is determined subelement, and the pixel that surpasses the first predetermined threshold value for brightness value is changed is defined as the conversion zone pixel that testing sample conversion zone is corresponding;
Primary importance is determined subelement, for the pixel set that adjacent conversion zone pixel is formed, is defined as the position of testing sample conversion zone.
Optionally, described position determination unit, comprising:
The 3rd brightness value is determined subelement, for obtaining the brightness value of described first each pixel of image;
The 4th brightness value is determined subelement, for obtaining the brightness value of described second each pixel of image;
Center pixel is determined subelement, and the pixel that surpasses the second predetermined threshold value for brightness value is changed is defined as center pixel corresponding to testing sample conversion zone center;
The first judging unit, for judging that whether described center pixel exists brightness value to change the edge pixel that surpasses the 3rd predetermined threshold value, obtains the first judged result around; Described the 3rd predetermined threshold value is less than described the second predetermined threshold value;
The second place is determined subelement, and for judgment result is that while being when described first, the pixel set that described center pixel and described edge pixel are formed, is defined as the position of testing sample conversion zone.
According to specific embodiment provided by the invention, the invention discloses following technique effect:
Image analysis method or the system of the SPR analyser of the embodiment of the present invention, by determining the position of each testing sample conversion zone of described biochip; According to described position, respectively described the first image and described the second image are divided into a plurality of local reaction images; Brightness change information according to the local reaction image of same position, carries out SPR analysis to determinand, can analyze having the determinand of a plurality of testing sample conversion zones on the biochip of a plurality of testing sample conversion zones.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment be briefly described below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skills, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the process flow diagram of the image analysis method embodiment 1 of SPR analyser of the present invention;
Fig. 2 is the process flow diagram of the image analysis method embodiment 2 of SPR analyser of the present invention;
Fig. 3 is the process flow diagram of the image analysis method embodiment 3 of SPR analyser of the present invention;
Fig. 4 is the process flow diagram of the image analysis method embodiment 4 of SPR analyser of the present invention;
Fig. 5 is the structural drawing of the image analysis system embodiment 1 of SPR analyser of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
For above-mentioned purpose of the present invention, feature and advantage can be become apparent more, below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
Fig. 1 is the process flow diagram of the image analysis method embodiment 1 of SPR analyser of the present invention.As shown in Figure 1, described method can comprise:
Step 101: the first image that obtains the biochip with a plurality of testing sample conversion zones; Described the first image is not for passing into the image of the biochip of determinand;
Biochip in the present embodiment, has a plurality of (up to a hundred) testing sample conversion zone.Because the total area of biochip is less, be generally several or tens square centimeters, therefore, while thering is up to a hundred testing sample conversion zones on biochip, for the technical requirement meeting of graphical analysis far above prior art.
Step 102: wait passing into determinand after probe on described biochip reacts, obtain the second image of described biochip;
Determinand can be to need detected fluid.Conventionally when testing, can pass through the biochemical characteristic of SPR technical Analysis determinand.
Probe on biochip, refers to the biomolecule identification film arranging on biochip.Each testing sample conversion zone can arrange different types of biomolecule identification film.If determinand and probe react, the SPR angle of conversion zone can change.And one of this variation demonstration is the most intuitively the variation of brightness.
Step 103: the position of determining each testing sample conversion zone of described biochip;
The model of biochip is different, and the number of the testing sample conversion zone arranging on it is also not quite similar.The size of the single testing sample conversion zone on some chip is not identical yet.Therefore, need to first determine the position of each testing sample conversion zone of described biochip, could the brightness before and after reaction change according to each testing sample conversion zone, determinand is carried out to SPR analysis.
Concrete, determine the position of each testing sample conversion zone of described biochip, can be according to sequence location and the size of the testing sample conversion zone that set for the biochip of every type in advance, to taking the image that described biochip obtains, divide; Also can be according to the brightness change information on the first image and the second image, number and the position of each testing sample conversion zone of biochip described in automatic analysis.
Step 104: respectively described the first image and described the second image are divided into a plurality of local reaction images according to described position;
The number of the local reaction image being specifically divided into, relevant with the response situation of the model of biochip or the probe of determinand and testing sample conversion zone.
Step 105: for the local reaction image of same position, compare by described the second image and described the first image, obtain the brightness change information of the local reaction image of same position;
The extent of reaction of the probe of determinand and testing sample conversion zone is stronger, and the brightness of the corresponding local reaction image in described the second image can be larger.
Step 106: according to described brightness change information, determinand is carried out to SPR analysis.
According to described brightness change information, can obtain the change information at SPR angle, and then change by detecting SPR angle, can obtain the information such as concentration, affinity, kinetic constant and specificity of determinand.Above-mentionedly by detecting SPR angle, change, the means that obtain the information such as concentration, affinity, kinetic constant and specificity of determinand are prior art, repeat no more herein.
In the present embodiment, by determining the position of each testing sample conversion zone of described biochip; According to described position, respectively described the first image and described the second image are divided into a plurality of local reaction images; Brightness change information according to the local reaction image of same position, carries out SPR analysis to determinand, can analyze having the determinand of a plurality of testing sample conversion zones on the biochip of a plurality of testing sample conversion zones.
It should be noted that, in the embodiment of the present invention, can adopt high-definition camera to obtain to have the first image and second image of the biochip of a plurality of testing sample conversion zones.Concrete, described high-definition camera can be that hardware sensor pixel is more than 1,300,000 camera.
Fig. 2 is the process flow diagram of the image analysis method embodiment 2 of SPR analyser of the present invention.As shown in Figure 2, described method can comprise:
Step 201: the first image that obtains the biochip with a plurality of testing sample conversion zones; Described the first image is not for passing into the image of the biochip of determinand;
Step 202: wait passing into determinand after probe on described biochip reacts, obtain the second image of described biochip;
Step 203: the positioning mark of the image that the described biochip of identification shooting obtains; Described positioning mark is that at least two characteristic areas on described biochip form;
Described positioning mark can be, on described biochip, telltale mark is set, for example, at cornerwise two end points places of biochip, adopt the pattern of feature obvious (round dot or triangle or rectangle etc.) to carry out mark.
Step 204: the size of determining described image according to described positioning mark;
According to described positioning mark, the length of described biochip and wide can be learnt, and then the size of described image can be determined.
Suppose that the positioning mark getting in step 203 lays respectively at the upper left corner and the upper right corner of biochip, corresponding coordinate is respectively (X1, Y1), (X2, Y2), the length of described image can be calculated according to X2-X1, and the wide of described image can be calculated according to Y2-Y1.
Step 205: the size according to default conversion zone size and described image, described image is divided into a plurality of regions, each region is corresponding with the position of a testing sample conversion zone.
Default conversion zone size can arrange according to actual needs.Also can obtain online according to the model of different biochips.Suppose that described image is of a size of 3*3=9 square centimeter, described default conversion zone is of a size of 1*1=1 square centimeter, can accordingly described image be divided into 9 regions.Each region is corresponding with a position.
Step 206: respectively described the first image and described the second image are divided into a plurality of local reaction images according to described position;
Step 207: for the local reaction image of same position, compare by described the second image and described the first image, obtain the brightness change information of the local reaction image of same position;
Step 208: according to described brightness change information, determinand is carried out to SPR analysis.
Fig. 3 is the process flow diagram of the image analysis method embodiment 3 of SPR analyser of the present invention.As shown in Figure 3, described method can comprise:
Step 301: the first image that obtains the biochip with a plurality of testing sample conversion zones; Described the first image is not for passing into the image of the biochip of determinand;
Step 302: wait passing into determinand after probe on described biochip reacts, obtain the second image of described biochip;
Step 303: the brightness value that obtains each pixel in described the first image;
Step 304: the brightness value that obtains each pixel in described the second image;
Step 305: the pixel that brightness value is changed over the first predetermined threshold value is defined as the conversion zone pixel that testing sample conversion zone is corresponding;
In the present embodiment, if the probe on determinand and biochip reacts, in corresponding region, on the second image, the pixel brightness value in this region can be greater than the pixel brightness value in this region on the first image.
Step 306: by the pixel set of adjacent conversion zone pixel formation, be defined as the position of testing sample conversion zone.
Adjacent conversion zone pixel refers to that adjacent in image coordinate and brightness value changes the pixel that surpasses the first predetermined threshold value.Conventionally, after the probe on determinand and biochip reacts, can form a brightness and change obvious region, the image pixel that this region is corresponding, can be just adjacent conversion zone pixel.The pixel set that these adjacent conversion zone pixels form, the position of the testing sample conversion zone namely reacting.
Step 307: respectively described the first image and described the second image are divided into a plurality of local reaction images according to described position;
Step 308: for the local reaction image of same position, compare by described the second image and described the first image, obtain the brightness change information of the local reaction image of same position;
Step 309: according to described brightness change information, determinand is carried out to SPR analysis.
Fig. 4 is the process flow diagram of the image analysis method embodiment 4 of SPR analyser of the present invention.As shown in Figure 4, described method can comprise:
Step 401: the first image that obtains the biochip with a plurality of testing sample conversion zones; Described the first image is not for passing into the image of the biochip of determinand;
Step 402: wait passing into determinand after probe on described biochip reacts, obtain the second image of described biochip;
Step 403: the brightness value that obtains each pixel in described the first image;
Step 404: the brightness value that obtains each pixel in described the second image;
Step 405: the pixel that brightness value is changed over the second predetermined threshold value is defined as center pixel corresponding to testing sample conversion zone center;
The second predetermined threshold value in the present embodiment can be than the large value of the first predetermined threshold value in the embodiment of Fig. 3.When the brightness value of pixel changes over the second predetermined threshold value, illustrate that the extent of reaction is herein more intense, be the center of testing sample conversion zone.
Step 406: judge that whether described center pixel exists brightness value to change the edge pixel that surpasses the 3rd predetermined threshold value, obtains the first judged result around; Described the 3rd predetermined threshold value is less than described the second predetermined threshold value;
The brightness value of the pixel that the edge image of testing sample conversion zone is corresponding changes for center pixel, and brightness changes less.In this step, by judging whether described center pixel exists brightness value to change the edge pixel that surpasses the 3rd predetermined threshold value around, center pixel in can determining step 405 is obtained by determinand and probe reaction, or a few brightness causing due to other accidental causes changes pixel greatly.That is,, if whether described center pixel exists brightness value to change the edge pixel that surpasses the 3rd predetermined threshold value, can determine to be testing sample conversion zone herein around; When described center pixel does not exist brightness value to change the edge pixel that surpasses the 3rd predetermined threshold value around, can determine that this center pixel is not obtained by determinand and probe reaction, but a few brightness causing due to other accidental causes changes pixel greatly.
Therefore, step 406 can improve the accuracy of image analysis method of the SPR analyser of the present embodiment.
Step 407: judgment result is that while being that the pixel set that described center pixel and described edge pixel are formed is defined as the position of testing sample conversion zone when described first.
In this step, by the pixel set of described center pixel and described edge pixel formation, refer to described center pixel and be positioned at described center pixel edge pixel around, forming a pixel set.
Step 408: for the local reaction image of same position, compare by described the second image and described the first image, obtain the brightness change information of the local reaction image of same position;
Step 409: according to described brightness change information, determinand is carried out to SPR analysis.
The present invention also provides a kind of image analysis system of SPR analyser.Fig. 5 is the structural drawing of the image analysis system embodiment 1 of SPR analyser of the present invention.As shown in Figure 5, described system can comprise:
The first image acquisition unit 501, for obtaining the first image of the biochip with a plurality of testing sample conversion zones; Described the first image is not for passing into the image of the biochip of determinand;
The second image acquisition unit 502, for wait passing into determinand after probe on described biochip reacts, obtains the second image of described biochip;
Comparing unit 505, for the local reaction image for same position, by described the second image and described the first image are compared, obtains the brightness change information of the local reaction image of same position;
In the present embodiment, by determining the position of each testing sample conversion zone of described biochip; According to described position, respectively described the first image and described the second image are divided into a plurality of local reaction images; Brightness change information according to the local reaction image of same position, carries out SPR analysis to determinand, can analyze having the determinand of a plurality of testing sample conversion zones on the biochip of a plurality of testing sample conversion zones.
It should be noted that, in the embodiment of the present invention, can adopt high-definition camera to obtain to have the first image and second image of the biochip of a plurality of testing sample conversion zones.Concrete, described high-definition camera can be that hardware sensor pixel is more than 1,300,000 camera.
In practical application, described the first image acquisition unit 501, can comprise:
The first Image Acquisition subelement is the first image that more than 1,300,000 camera obtains the biochip with a plurality of testing sample conversion zones for adopting hardware sensor pixel;
Described the second image acquisition unit, comprising:
The second Image Acquisition subelement is the second image that more than 1,300,000 camera obtains described biochip for adopting hardware sensor pixel.
Described position determination unit 503, can comprise:
Positioning mark recognin unit, for identifying the positioning mark of taking the image that described biochip obtains; Described positioning mark is that at least two characteristic areas on described biochip form;
Size is determined subelement, for determine the size of described image according to described positioning mark;
Region Segmentation subelement, for according to the size of default conversion zone size and described image, is divided into a plurality of regions by described image, and each region is corresponding with the position of a testing sample conversion zone.
Described position determination unit 503, can also comprise:
The first brightness value obtains subelement, for obtaining the brightness value of described first each pixel of image;
The second brightness value obtains subelement, for obtaining the brightness value of described second each pixel of image;
Conversion zone pixel is determined subelement, and the pixel that surpasses the first predetermined threshold value for brightness value is changed is defined as the conversion zone pixel that testing sample conversion zone is corresponding;
Primary importance is determined subelement, for the pixel set that adjacent conversion zone pixel is formed, is defined as the position of testing sample conversion zone.
Described position determination unit 503, can also comprise:
The 3rd brightness value is determined subelement, for obtaining the brightness value of described first each pixel of image;
The 4th brightness value is determined subelement, for obtaining the brightness value of described second each pixel of image;
Center pixel is determined subelement, and the pixel that surpasses the second predetermined threshold value for brightness value is changed is defined as center pixel corresponding to testing sample conversion zone center;
The first judging unit, for judging that whether described center pixel exists brightness value to change the edge pixel that surpasses the 3rd predetermined threshold value, obtains the first judged result around; Described the 3rd predetermined threshold value is less than described the second predetermined threshold value;
The second place is determined subelement, and for judgment result is that while being when described first, the pixel set that described center pixel and described edge pixel are formed, is defined as the position of testing sample conversion zone.
In this instructions, each embodiment adopts the mode of going forward one by one to describe, and each embodiment stresses is the difference with other embodiment, between each embodiment identical similar part mutually referring to.For the disclosed system of embodiment, because it corresponds to the method disclosed in Example, so description is fairly simple, relevant part partly illustrates referring to method.
Applied specific case herein principle of the present invention and embodiment are set forth, the explanation of above embodiment is just for helping to understand method of the present invention and core concept thereof; Meanwhile, for one of ordinary skill in the art, according to thought of the present invention, all will change in specific embodiments and applications.In sum, this description should not be construed as limitation of the present invention.
Claims (10)
1. an image analysis method for SPR analyser, is characterized in that, comprising:
Obtain the first image of the biochip with a plurality of testing sample conversion zones; Described the first image is not for passing into the image of the biochip of determinand;
Wait passing into determinand after probe on described biochip reacts, obtain the second image of described biochip;
Determine the position of each testing sample conversion zone of described biochip;
According to described position, respectively described the first image and described the second image are divided into a plurality of local reaction images;
For the local reaction image of same position, by described the second image and described the first image, compare, obtain the brightness change information of the local reaction image of same position;
According to described brightness change information, determinand is carried out to SPR analysis.
2. method according to claim 1, is characterized in that, described in obtain the first image of the biochip with a plurality of testing sample conversion zones, comprising:
Employing hardware sensor pixel is the first image that more than 1,300,000 cameras obtains the biochip with a plurality of testing sample conversion zones;
Described the second image that obtains described biochip, comprising:
Employing hardware sensor pixel is the second image that more than 1,300,000 cameras obtains described biochip.
3. method according to claim 1, is characterized in that, the described position of determining each testing sample conversion zone of described biochip, comprising:
The positioning mark of the image that the described biochip of identification shooting obtains; Described positioning mark is that at least two characteristic areas on described biochip form;
According to described positioning mark, determine the size of described image;
Size according to default conversion zone size and described image, is divided into a plurality of regions by described image, and each region is corresponding with the position of a testing sample conversion zone.
4. method according to claim 1, is characterized in that, the described position of determining each testing sample conversion zone of described biochip, comprising:
Obtain the brightness value of each pixel in described the first image;
Obtain the brightness value of each pixel in described the second image;
The pixel that brightness value is changed over the first predetermined threshold value is defined as the conversion zone pixel that testing sample conversion zone is corresponding;
By the pixel set of adjacent conversion zone pixel formation, be defined as the position of testing sample conversion zone.
5. method according to claim 1, is characterized in that, the described position of determining each testing sample conversion zone of described biochip, comprising:
Obtain the brightness value of each pixel in described the first image;
Obtain the brightness value of each pixel in described the second image;
The pixel that brightness value is changed over the second predetermined threshold value is defined as center pixel corresponding to testing sample conversion zone center;
Judge that whether described center pixel exists brightness value to change the edge pixel that surpasses the 3rd predetermined threshold value, obtains the first judged result around; Described the 3rd predetermined threshold value is less than described the second predetermined threshold value;
Judgment result is that while being that the pixel set that described center pixel and described edge pixel are formed is defined as the position of testing sample conversion zone when described first.
6. an image analysis system for SPR analyser, is characterized in that, comprising:
The first image acquisition unit, for obtaining the first image of the biochip with a plurality of testing sample conversion zones; Described the first image is not for passing into the image of the biochip of determinand;
The second image acquisition unit, for wait passing into determinand after probe on described biochip reacts, obtains the second image of described biochip;
Position determination unit, for determining the position of each testing sample conversion zone of described biochip;
Image cutting unit, for being divided into a plurality of local reaction images by described the first image and described the second image respectively according to described position;
Comparing unit, for the local reaction image for same position, by described the second image and described the first image are compared, obtains the brightness change information of the local reaction image of same position;
Analytic unit, for according to described brightness change information, carries out SPR analysis to determinand.
7. system according to claim 6, is characterized in that, described the first image acquisition unit, comprising:
The first Image Acquisition subelement is the first image that more than 1,300,000 camera obtains the biochip with a plurality of testing sample conversion zones for adopting hardware sensor pixel;
Described the second image acquisition unit, comprising:
The second Image Acquisition subelement is the second image that more than 1,300,000 camera obtains described biochip for adopting hardware sensor pixel.
8. system according to claim 6, is characterized in that, described position determination unit, comprising:
Positioning mark recognin unit, for identifying the positioning mark of taking the image that described biochip obtains; Described positioning mark is that at least two characteristic areas on described biochip form;
Size is determined subelement, for determine the size of described image according to described positioning mark;
Region Segmentation subelement, for according to the size of default conversion zone size and described image, is divided into a plurality of regions by described image, and each region is corresponding with the position of a testing sample conversion zone.
9. system according to claim 6, is characterized in that, described position determination unit, comprising:
The first brightness value obtains subelement, for obtaining the brightness value of described first each pixel of image;
The second brightness value obtains subelement, for obtaining the brightness value of described second each pixel of image;
Conversion zone pixel is determined subelement, and the pixel that surpasses the first predetermined threshold value for brightness value is changed is defined as the conversion zone pixel that testing sample conversion zone is corresponding;
Primary importance is determined subelement, for the pixel set that adjacent conversion zone pixel is formed, is defined as the position of testing sample conversion zone.
10. system according to claim 6, is characterized in that, described position determination unit, comprising:
The 3rd brightness value is determined subelement, for obtaining the brightness value of described first each pixel of image;
The 4th brightness value is determined subelement, for obtaining the brightness value of described second each pixel of image;
Center pixel is determined subelement, and the pixel that surpasses the second predetermined threshold value for brightness value is changed is defined as center pixel corresponding to testing sample conversion zone center;
The first judging unit, for judging that whether described center pixel exists brightness value to change the edge pixel that surpasses the 3rd predetermined threshold value, obtains the first judged result around; Described the 3rd predetermined threshold value is less than described the second predetermined threshold value;
The second place is determined subelement, and for judgment result is that while being when described first, the pixel set that described center pixel and described edge pixel are formed, is defined as the position of testing sample conversion zone.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310673597.1A CN103700096A (en) | 2013-12-11 | 2013-12-11 | Method and system for analyzing images of SPR (Surface Plasmon Resonance) analyzer |
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| CN201310673597.1A CN103700096A (en) | 2013-12-11 | 2013-12-11 | Method and system for analyzing images of SPR (Surface Plasmon Resonance) analyzer |
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| CN107330899A (en) * | 2017-06-21 | 2017-11-07 | 新疆大学 | A kind of dividing method and device of biochip reflected light image |
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