CN102703562A - Method for prompting extraction of linaloe oil by utilizing flora fermentation - Google Patents
Method for prompting extraction of linaloe oil by utilizing flora fermentation Download PDFInfo
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- CN102703562A CN102703562A CN2012101975090A CN201210197509A CN102703562A CN 102703562 A CN102703562 A CN 102703562A CN 2012101975090 A CN2012101975090 A CN 2012101975090A CN 201210197509 A CN201210197509 A CN 201210197509A CN 102703562 A CN102703562 A CN 102703562A
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- agilawood
- flora
- linaloe oil
- agalloch eaglewood
- fermentation
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- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000000855 fermentation Methods 0.000 title claims abstract description 13
- 230000004151 fermentation Effects 0.000 title claims abstract description 13
- 238000000605 extraction Methods 0.000 title claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- 241000271309 Aquilaria crassna Species 0.000 claims description 34
- 241000894006 Bacteria Species 0.000 claims description 12
- 239000000284 extract Substances 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 8
- 241000233866 Fungi Species 0.000 claims description 7
- 241000190144 Lasiodiplodia theobromae Species 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 3
- 241000839434 Diplodia sp. Species 0.000 claims description 3
- 241000223218 Fusarium Species 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 235000005979 Citrus limon Nutrition 0.000 claims description 2
- 244000131522 Citrus pyriformis Species 0.000 claims description 2
- 235000009414 Elaeocarpus kirtonii Nutrition 0.000 claims description 2
- 229920001214 Polysorbate 60 Polymers 0.000 claims description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 2
- 244000236151 Tabebuia pallida Species 0.000 claims description 2
- 235000013584 Tabebuia pallida Nutrition 0.000 claims description 2
- 240000008866 Ziziphus nummularia Species 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 239000006071 cream Substances 0.000 claims description 2
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- 239000012895 dilution Substances 0.000 claims description 2
- 239000012467 final product Substances 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 239000012533 medium component Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000012466 permeate Substances 0.000 claims description 2
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- 239000001632 sodium acetate Substances 0.000 claims description 2
- 235000017281 sodium acetate Nutrition 0.000 claims description 2
- 239000004317 sodium nitrate Substances 0.000 claims description 2
- 229940001516 sodium nitrate Drugs 0.000 claims description 2
- 235000010344 sodium nitrate Nutrition 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 8
- 239000002994 raw material Substances 0.000 abstract description 4
- 238000002360 preparation method Methods 0.000 abstract description 3
- 239000007787 solid Substances 0.000 abstract description 3
- 239000003921 oil Substances 0.000 abstract 4
- 238000012258 culturing Methods 0.000 abstract 3
- 239000000341 volatile oil Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241001265525 Edgeworthia chrysantha Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 241001533085 Aquilaria sinensis Species 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
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- Fats And Perfumes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for prompting the extraction of linaloe oil by utilizing flora fermentation. By using the method, on the basis of not increasing the supply of a raw material, the unit yield of the linaloe oil can be greatly improved, and an agilawood material is subjected to mixed fermentation and conversion treatment through extracting and culturing a flora preparation by utilizing agilawood endophyte, so as to obviously promote the extraction rate of the linaloe oil. The method comprises the following culture steps of: extracting the agilawood endophyte, purifying a cultured primary solid test-tube strain, culturing a secondary strain of a flora by a combinative liquid shaking table, culturing an expanded tertiary liquid strain of a seed of the flora through a shaking table, and fermenting with the agilawood material in a mixed way. The extraction rate of the converted agilawood material is greatly improved on an original basis, so that the yield of the linaloe oil is increased from average 0.23% to 0.48% on the basis of not increasing the supply of an agilawood raw material; not only are lots of the agilawood materials saved, but also an agilawood essential oil product with a high added value is created; export foreign exchange earnings are expanded; and the method has considerable economic benefit and social benefit.
Description
Technical field
The present invention relates to biological technical field; A kind of process method of utilizing multiple advantage fungi in the agalloch eaglewood endophyte or flora and agalloch eaglewood material to carry out mixed fermentation; Can significantly promote the further conversion of agalloch eaglewood material by this method, thereby increase substantially the extraction yield of linaloe oil.
Background technology
Homemade agalloch eaglewood is the pathology timber that thymelaeceae eaglewood Lignum Aquilariae Resinatum Aquilaria sinensis (Lour.) Gilg contains black resin, can produce through modes such as nature Edgeworthia chrysantha or artificial Edgeworthia chrysanthas.In China, Lignum Aquilariae Resinatum is unique plant resources of producing homemade agalloch eaglewood, is the distinctive precious medicinal plant of China.The linaloe oil that from agalloch eaglewood, extracts is again the agalloch eaglewood product of high added value, and along with the medicinal of linaloe oil and as the significantly increase of beauty and health care consumption, market is the trend that supply falls short of demand to the demand of linaloe oil series product.At present the first-class linaloe oil price in domestic and international market up to 300 yuan/gram, in great demand especially on middle east market, the foreign exchange earning prospect is very wide.
Because the fat content in the common agalloch eaglewood material is very low, extraction yield is merely about 0.23% usually, and very precious so output is few, therefore the domestic and international market is that supply falls short of demand always, and the agalloch eaglewood cost of material is also rising steadily at present; Become very urgent subject through the extraction yield of raising linaloe oil and the economic benefit of product.
In order to alleviate the imbalance between supply and demand in market; Improve the output and the economic benefit of linaloe oil; According to the fragrant rule of natural agalloch eaglewood natural birth with form mechanism, from the wild agalloch eaglewood that is growing, extract some agalloch eaglewood endogenetic fungus that can promote that agalloch eaglewood generates, ferment with the agalloch eaglewood material mixing through the purified special bacterial colony preparation of cultivating into of optimum combination advantage aroma former strain; Experiment shows the extraction yield that can promote and improve linaloe oil significantly; Extraction yield brings up to 0.48% from original 0.23%, and its output transforms through the flora preparation fermentation and is doubled manyly, gropes and test out operational path and the method that significantly improves linaloe oil output thus.
Summary of the invention
According to the invention on the basis that does not increase the agalloch eaglewood raw material supply, significantly improve the unit output of linaloe oil, utilize the agalloch eaglewood endophyte that the agalloch eaglewood material is carried out mixed fermentation for this reason and promote conversion processing, thereby significantly promote the yield of linaloe oil,
Summary of the invention is; In the natural agalloch eaglewood that is growing, extract many strains and have the fungi that advantage is produced fragrant ability; Coloured two spore bacterium (Diplodia sp.); Diplodia natalensis (Botryosphaeria rhodina); Sickle-like bacteria (fusarium) branch top spore mould (acreonium), light are deceived shell bacterial strains such as (fleischhakii) and are original seed through sterilization respectively, purifying cultivation, and its process is following;
The solid test tube strains that the agalloch eaglewood endophyte extracts, purifying is cultivated → combination liquid shaking table is cultivated the liquid spawn of flora bacterial classification → flora seed expansion and is cultivated through shaking table.
Embodiment
To from the many strains live body agalloch eaglewood endophyte the agalloch eaglewood plantation, Longnan, Qing Wan town, Beiliu City, Guangxi, extract the multiple fungi that produces fragrant advantageous ability that has respectively; Coloured two spore bacterium (Diplodia sp.), Diplodia natalensis (Botryosphaeria rhodina), sickle-like bacteria (fusarium) branch top spore mould (acreonium), light is deceived shell (fleischhakii)
Above-mentioned bacterial strains through extract respectively, sterilize and be inoculated in the seed culture medium of wort solid agar (glucose 10g/L, an albumen 10g/L, yeast extract 5g/L, NaCl10g/L, agar 20g/L pH7.0) puts 28 ℃ and cultivates and became the test tube original seed in 7 days.
Cutting youngster in the test tube plants the original seed fungi and inserts 100ml liquid nutrient medium (glucose 20g/L, an albumen 10g/L, yeast powder 5g/L respectively; Carnis Bovis seu Bubali cream 10g/L, sodium acetate 2g/L, lemon wild jujube g/L; SODIUMNITRATE 2mLpH6.5) triangular flask of 100ml * 10 is cultivated 200～250 rev/mins of rotating speeds 32 ℃ of combinations; Shaking table was cultivated 7 days, got the composite bacteria nutrient solution.
Get composite bacteria nutrient solution 500ml; Insert 10000ml in the liquid spawn culture medium that enlarges, (identical with the aforesaid liquid medium component, it is measured according to the corresponding increase of 10000ml; 28～32 ℃ of fermentation culture of room temperature; 200～250 rev/mins, shaking table was cultivated 5 days, composite bacteria or nutrient solution after obtaining enlarging.
Fermentative prepn with the agalloch eaglewood material; 200 kilograms of agalloch eaglewood siccatives; Pulverize to chip, insert fermentor tank, add flora nutrient solution 1000ml; (with 150 kilograms sterilized water dilution) permeate agent Tween-60 adds 200 gram mixings; The humidity of compound is pinched agglomerating rare spilling water with hand and is got final product, and 32～37 ℃ of fermentation reactions of temperature 15 days promote that fully the whitewood's composition in the agalloch eaglewood material obtains effecting reaction and conversion.
Agalloch eaglewood material after the conversion is again through the solvent extraction linaloe oil; Its extraction yield and former unleavened agalloch eaglewood material extraction contrast are improved largely, thereby on the basis that does not increase the agalloch eaglewood raw material supply, the yield of linaloe oil brings up to 0.48% from average 0.23%; Not only saved a large amount of agalloch eaglewood materials; And significantly increase production and high added value linaloe oil product, enlarged foreign exchange earning, have very considerable economic and social benefit.
Claims (5)
1. utilize the flora fermentation to promote the method that linaloe oil extracts; Its inventive features is meant it can is to utilize single bacterial strain, also can be the flora that several kinds to tens kinds strain combinations are cultivated, through with the method for agalloch eaglewood material combined ferment, be used for significantly promoting and improving the extraction yield of linaloe oil.
2. utilize the flora fermentation to promote that four kinds of strain characteristicses of the method that linaloe oil extracts are sufficient; In the natural agalloch eaglewood that is growing, extract the multiple fungi that produces fragrant ability that has; Coloured two spore bacterium (Diplodia sp.), Diplodia natalensis (Botryosphaeria rhodina), sickle-like bacteria (fusarium) branch top spore mould (acreonium), light are deceived shell bacterial strains such as (fleischhakii) and are original seed through sterilization respectively, purifying cultivation.
3. several kinds of original seed fungies that cut in the test tube are inserted 100ml liquid nutrient medium (glucose 20g/L, an albumen 10g/L, yeast powder 5g/L respectively; Carnis Bovis seu Bubali cream 10g/L, sodium acetate 2g/L, lemon wild jujube g/L; SODIUMNITRATE 2mLpH6.5) triangular flask of 100ml * 10 is cultivated 200～250 rev/mins of rotating speeds 32 ℃ of combinations; Shaking table was cultivated 7 days, got the composite bacteria nutrient solution.
4. get composite bacteria nutrient solution 500ml; Insert 10000ml in the liquid spawn culture medium that enlarges, (identical with the aforesaid liquid medium component, it is measured according to the corresponding increase of 10000ml; 28～32 ℃ of fermentation culture of room temperature; 200～250 rev/mins, shaking table was cultivated 5 days, composite bacteria or nutrient solution after obtaining enlarging.
5. the fermentative prepn of agalloch eaglewood material; 200 kilograms of agalloch eaglewood siccatives; Pulverize to chip, insert fermentor tank, add flora nutrient solution 1000ml; (with 150 kilograms sterilized water dilution) permeate agent Tween-60 adds 200 gram mixings; The humidity of compound is pinched agglomerating not loosing with hand and is got final product, 32～37 ℃ of fermentation reactions of temperature 15 days, and the whitewood's composition in the promotion agalloch eaglewood material is fully reacted and is transformed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101975090A CN102703562A (en) | 2012-06-15 | 2012-06-15 | Method for prompting extraction of linaloe oil by utilizing flora fermentation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012101975090A CN102703562A (en) | 2012-06-15 | 2012-06-15 | Method for prompting extraction of linaloe oil by utilizing flora fermentation |
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| Publication Number | Publication Date |
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| CN102703562A true CN102703562A (en) | 2012-10-03 |
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| CN2012101975090A Pending CN102703562A (en) | 2012-06-15 | 2012-06-15 | Method for prompting extraction of linaloe oil by utilizing flora fermentation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320232A (en) * | 2013-07-19 | 2013-09-25 | 中国医学科学院药用植物研究所海南分所 | Fermentation and extraction method of agilawood essential oil by utilization of mixed enzymic preparations |
| CN106701322A (en) * | 2017-02-03 | 2017-05-24 | 海南大学 | Method for extracting fermented morinda citrifolia seed essential oil |
| CN111821233A (en) * | 2020-06-30 | 2020-10-27 | 广州伽能生物科技有限公司 | Plant whitening natural active compound composition and preparation method and application thereof |
| CN115772446A (en) * | 2022-11-17 | 2023-03-10 | 钟伟杰 | Agilawood essential oil and preparation method and application thereof |
| CN116554964A (en) * | 2023-05-06 | 2023-08-08 | 华南协同创新研究院 | A method for increasing the aroma of isolated agarwood |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101229282A (en) * | 2008-02-02 | 2008-07-30 | 怒江享靓园林有限责任公司 | Technology of extracting ligaloes milk from agalloch eaglewood |
-
2012
- 2012-06-15 CN CN2012101975090A patent/CN102703562A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101229282A (en) * | 2008-02-02 | 2008-07-30 | 怒江享靓园林有限责任公司 | Technology of extracting ligaloes milk from agalloch eaglewood |
Non-Patent Citations (1)
| Title |
|---|
| 弓宝等: "考察不同发酵时间对沉香精油提取率的影响", 《香料香精化妆品》, no. 6, 31 December 2011 (2011-12-31), pages 17 - 18 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103320232A (en) * | 2013-07-19 | 2013-09-25 | 中国医学科学院药用植物研究所海南分所 | Fermentation and extraction method of agilawood essential oil by utilization of mixed enzymic preparations |
| CN106701322A (en) * | 2017-02-03 | 2017-05-24 | 海南大学 | Method for extracting fermented morinda citrifolia seed essential oil |
| CN106701322B (en) * | 2017-02-03 | 2021-09-10 | 海南大学 | Extraction method of fermented noni seed essential oil |
| CN111821233A (en) * | 2020-06-30 | 2020-10-27 | 广州伽能生物科技有限公司 | Plant whitening natural active compound composition and preparation method and application thereof |
| CN111821233B (en) * | 2020-06-30 | 2021-06-15 | 广州伽能生物科技有限公司 | Plant whitening natural active compound composition and preparation method and application thereof |
| CN115772446A (en) * | 2022-11-17 | 2023-03-10 | 钟伟杰 | Agilawood essential oil and preparation method and application thereof |
| CN116554964A (en) * | 2023-05-06 | 2023-08-08 | 华南协同创新研究院 | A method for increasing the aroma of isolated agarwood |
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Application publication date: 20121003 |