CN102649936A - Compound microorganism fungicide for improving water quality of culturing water body and preparation method - Google Patents
Compound microorganism fungicide for improving water quality of culturing water body and preparation method Download PDFInfo
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- CN102649936A CN102649936A CN2012101321015A CN201210132101A CN102649936A CN 102649936 A CN102649936 A CN 102649936A CN 2012101321015 A CN2012101321015 A CN 2012101321015A CN 201210132101 A CN201210132101 A CN 201210132101A CN 102649936 A CN102649936 A CN 102649936A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 63
- 238000002360 preparation method Methods 0.000 title claims abstract description 52
- 244000005700 microbiome Species 0.000 title claims abstract description 40
- 238000012258 culturing Methods 0.000 title abstract description 13
- 230000000855 fungicidal effect Effects 0.000 title abstract description 7
- 239000000417 fungicide Substances 0.000 title abstract description 7
- 150000001875 compounds Chemical class 0.000 title abstract 4
- 241000894006 Bacteria Species 0.000 claims abstract description 77
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 42
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 39
- 239000007787 solid Substances 0.000 claims abstract description 37
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 21
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 239000002609 medium Substances 0.000 claims description 39
- 238000009360 aquaculture Methods 0.000 claims description 37
- 244000144974 aquaculture Species 0.000 claims description 37
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims description 29
- 230000006872 improvement Effects 0.000 claims description 29
- 239000002994 raw material Substances 0.000 claims description 27
- 241000108664 Nitrobacteria Species 0.000 claims description 18
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 238000009630 liquid culture Methods 0.000 claims description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 6
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 6
- 241001495402 Nitrococcus Species 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 6
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 4
- -1 amine molybdate Chemical class 0.000 claims description 4
- 150000002823 nitrates Chemical class 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- 235000019270 ammonium chloride Nutrition 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- 230000009849 deactivation Effects 0.000 claims description 3
- 239000008121 dextrose Substances 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 229940045641 monobasic sodium phosphate Drugs 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- 235000017550 sodium carbonate Nutrition 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 3
- 235000010288 sodium nitrite Nutrition 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 241001609931 bacterium 20 Species 0.000 claims 3
- 241000933856 bacterium 8 Species 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 12
- 238000001035 drying Methods 0.000 abstract description 3
- 244000063299 Bacillus subtilis Species 0.000 abstract 3
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 3
- 239000004310 lactic acid Substances 0.000 abstract 3
- 230000001546 nitrifying effect Effects 0.000 abstract 2
- 239000001888 Peptone Substances 0.000 abstract 1
- 108010080698 Peptones Proteins 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 1
- 235000015278 beef Nutrition 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 235000019319 peptone Nutrition 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 241000251468 Actinopterygii Species 0.000 description 8
- 230000008569 process Effects 0.000 description 7
- 241000195493 Cryptophyta Species 0.000 description 6
- 230000002068 genetic effect Effects 0.000 description 6
- 230000000813 microbial effect Effects 0.000 description 5
- 241000192700 Cyanobacteria Species 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 241000700141 Rotifera Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 241001247197 Cephalocarida Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- QRSFFHRCBYCWBS-UHFFFAOYSA-N [O].[O] Chemical compound [O].[O] QRSFFHRCBYCWBS-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 238000004176 ammonification Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000002309 gasification Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000003403 water pollutant Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a compound microorganism fungicide for improving the water quality of a culturing water body and a preparation method. The compound microorganism fungicide is prepared by taking the strains by weight percent: bacillus subtilis, yeast, lactic acid bacteria, photosynthetic bacteria, nitrifying bacteria, bacillus licheniformis, and the like. The preparation method comprises the following steps of: firstly, culturing the bacillus subtilis and bacillus licheniformis: culturing the bacillus subtilis and bacillus licheniformis on a beef extract peptone culture medium; secondly, culturing the yeast: culturing the yeast on a lactic acid-potato-glucose culture medium; thirdly, culturing the lactic acid bacteria: culturing the lactic acid bacteria on a wort culture medium, inoculating on a triangular flask for culturing, and finally inoculating on a solid culture medium for culturing; fourthly, culturing the photosynthetic bacteria; fifthly, culturing the nitrifying bacteria; and sixthly, stirring and mixing the cultured strains by weight ratio, and drying at low temperature, thereby obtaining the compound microorganism fungicide. The formula is reasonable; the use is convenient; water is uniformly splashed; the method is easily performed; and the operation is convenient.
Description
Technical field
The invention belongs to microbial technology field; More specifically relate to a kind of complex microorganism preparations that is used for the aquaculture water water quality improvement; The preparation method who also relates to this biotechnological formulation simultaneously, said preparation is applicable to the breed of fingerling and adult fish in the aquaculture wateies such as lake, reservoir, pond.
Background technology
Development along with culture fishery; The batch production high-density breeding needs a large amount of feeds that drop into; The remaining movement with aquatic animal of bait is dissolved in the water, and aquaculture water is polluted, and causes the objectionable impurities increases such as organism, NH3, H2S in the water body; Chemical oxygen demand (COD) and BOD (BOD) are high simultaneously, can effectively eliminate above-mentioned negative impact through using microbiobacterial agent.Microbial preparation is applied to improve in the aquaculture water ecological setting; Suppress killing microorganism, it also can be used as fodder additives, the extra-nutrition composition; Improve cultivated animals gi tract profitable strain; Reach the purpose of ecological diseases prevention, make the breeding production benign development, obtain better economic benefit and ecological effect.Microbial preparation can effectively reduce nitrite in the cultivation water, ammonia nitrogen, hydrogen sulfide isoconcentration, suppresses harmful microorganism breeding and growth in the water body, purifies water.The metabolism of mikrobe in the preparation own has gasification, ammonification, phosphorus decomposing, denitrification, nitrated and nitrogen fixation; Can pollutent be decomposed into non-toxic substances such as carbonic acid gas, nitrate salt, vitriol; And then be used by the algae in the water body, reach the purpose that purifies water.Its population competition performance suppresses pathogenic bacterium, and useful mattress and host's mucous epithelium are combined closely and generated the compactness mycoderm, form microbial barrier, and the probiotics that has produces microbiotic and bacteriocin kill pathogenic bacteria.
Microbial preparation has little, the high efficiency of investment, advantage such as easy to use.Can full pool spilling head, also can be as fodder additives.Nontoxic, harmless, drug residue free, do not produce resistance, life-time service can reduce antibiotic usage quantity in the breeding process, reduces disease and takes place, and the sewage environmental pollution of discharging is also less.
Summary of the invention
The present invention uses chemical fertilizer, fertilizer in a large number to present aquaculture water; Cause water quality overfertilization, eutrophication serious, bring out that fish raises the nose above water to breathe and problem such as multiple disease, the objective of the invention is to be to provide a kind of complex microorganism preparations that is used for the aquaculture water water quality improvement; Prescription rationally; Easy to use, pollution-free to water body, cost is low.
Another object of the present invention is to be to provide a kind of preparation method who is used for the complex microorganism preparations of aquaculture water water quality improvement, and easy to implement the method, easy and simple to handle, production cost is low.
Technology of the present invention is achieved in that
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it is processed by the raw material of following massfraction:
Described subtilis (seeing the genetic resources table), yeast (seeing the genetic resources table), milk-acid bacteria (seeing the genetic resources table), photosynthetic bacterium (seeing the genetic resources table), nitrobacteria (seeing the genetic resources table), Bacillus licheniformis (seeing the genetic resources table).
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it processes (preferable range) by the raw material of following massfraction:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it processes (optimum range) by the raw material of following massfraction:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it processes (best proportioning) by the raw material of following massfraction:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it processes (best proportioning) by the raw material of following massfraction:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it processes (best proportioning) by the raw material of following massfraction:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it processes (best proportioning) by the raw material of following massfraction:
A kind of preparation method who is used for the complex microorganism preparations of aquaculture water water quality improvement the steps include:
A, at first be the cultivation of subtilis, Bacillus licheniformis: subtilis, Bacillus licheniformis are on beef-protein medium; 28-30 ℃; Making primary inclined plane cultivates; Be inoculated into triangular flask then and do vibration secondary liquid culture, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium.
B, secondly be saccharomycetic cultivation: yeast is on lactic acid-yam-dextrose culture-medium; 28-30 ℃; Make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
The cultivation of C, milk-acid bacteria: milk-acid bacteria on malt extract medium, 28-30 ℃, make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-5.0 * 10
8Individual/the gram solid medium;
The cultivation of D, photosynthetic bacterium: photosynthetic bacterium stoste is inoculated in the liquid test tube with cover, 28-30 ℃, illumination cultivation 3-5 days, gets the photosynthetic bacterium bacteria suspension; Get the bacteria suspension of 1% (volume ratio) and put into the substratum of 500ml triangular flask deactivation; With Fan Shi substratum multiplication culture; 28-30 ℃ of constant-temperature shaking shaking table; Cultivated 36-72 hour; Get the secondary pure growth of photosynthetic bacterium; The photosynthetic bacterium secondary seed of getting 1% (volume ratio) is inoculated in fermentation propagation in the substratum of being made up of 2% (mass ratio) sodium acetate, 1% (mass ratio) ammonium chloride, 0.1% (mass ratio) potassium primary phosphate, 0.5% (mass ratio) sal epsom, 0.2 (mass ratio) sodium-chlor, 3% (mass ratio) amine molybdate, 0.01% (mass ratio) yeast extract, 0.05 (mass ratio) calcium chloride, 0.05% (mass ratio) iron(ic)chloride, 28-30 ℃ of static cultivation of illumination or not illumination shaking culture, liquid photosynthetic bacterium fermenting agent; Again liquid photosynthetic bacterium fermenting agent is inoculated on the solid medium, gets the bacterium number and reach 1.0-2.0 * 10
8The solid photosynthetic bacteria fermenting agent of individual/ml;
The cultivation of E, nitrobacteria: nitrobacteria comprises nitrococcus and nitrifier; Respectively the ammonia-state nitrogen in the water is converted into nitrite and nitrate salt; 24 ℃; Make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
Described nitrococcus substratum: ammonium sulfate
2SO
40.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, potassium hydrogenphosphate 1g, sal epsom 0.03g, calcium chloride 7.5g, zero(ppm) water 1000ml, PH nature, solid medium add 5% (mass ratio) agar;
Described nitrifier substratum: Sodium Nitrite 1g, sal epsom 0.03g, manganous sulfate 0.01g, potassium hydrogenphosphate 0.75g, yellow soda ash 1g, SODIUM PHOSPHATE, MONOBASIC N0.25g, zero(ppm) water 1000ml, PH nature, solid medium add 5% (mass ratio) agar;
F, subtilis, yeast, milk-acid bacteria, photosynthetic bacterium, nitrobacteria, bacillus licheniformis agent that above-mentioned A, B, C, D, E step are cultivated mix by certain mass ratio; Low temperature (≤60 ℃) drying; Moisture content is controlled at below 20%, just obtains complex microorganism preparations.
The present invention compared with prior art has the following advantages and effect:
Profitable strains such as subtilis, Bacillus licheniformis, photosynthetic bacterium, milk-acid bacteria, yeast, nitrobacteria are carried out scientific and reasonable integration, a kind of complex micro organism fungicide that the uses advanced production technique produces.These cultivate the complex micro organism fungicide form through special methods, have share weal or woe, have complementary functions, the characteristics of cooperation.After applying water body; Utilize the mikrobe metabolic activity, to organic pollutant in the water shift, conversion and Degradation, reduce water pollutant concentration; Objectionable impuritiess such as ammonia nitrogen, nitrous acid, hydrogen sulfide in the collaborative water of decomposition environment are improved the anaerobic environment at the bottom of the pond; Improve the absorb efficient of unicellular algae to nutritive element, promote to swim but the growth of born of the same parents algae, balance algae phase increases the algae variety, suppresses the growth of harmful bacterium.Photosynthetic bacteria utilizes in luminous energy and the water simple inorganics to carry out photosynthesis, fixedly in the water element such as nitrogen and phosphorus the time, produces oxygen oxygen level in the increase water body, raising water transparency; Stablize water pH value.Keep culturing the balance of little ecology, make aquaculture water present " fertilizer ", " work ", " tender ", " feeling well "; Method of use is simple simultaneously, and is convenient, reduces labour cost.
Embodiment
Embodiment 1:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it is processed by the raw material of following massfraction:
A kind of preparation method who is used for the complex microorganism preparations of aquaculture water water quality improvement the steps include:
A, at first be the cultivation of subtilis, Bacillus licheniformis: subtilis, Bacillus licheniformis are on beef-protein medium; 28 or 29 or 30 ℃; Making primary inclined plane cultivates; Be inoculated into triangular flask then and do vibration secondary liquid culture, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium.
B, secondly be saccharomycetic cultivation: yeast is on lactic acid-yam-dextrose culture-medium; 28 or 29 or 30 ℃; Make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
The cultivation of C, milk-acid bacteria: milk-acid bacteria on malt extract medium, 28 or 29 or 30 ℃, make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-5.0 * 10
8Individual/the gram solid medium;
The cultivation of D, photosynthetic bacterium: photosynthetic bacterium stoste is inoculated in the liquid test tube with cover, 28-30 ℃, illumination cultivation 3-5 days, gets the photosynthetic bacterium bacteria suspension; Get the bacteria suspension of 1% (volume ratio) and put into the substratum of 500ml triangular flask deactivation; With Fan Shi substratum multiplication culture; 28-30 ℃ of constant-temperature shaking shaking table; Cultivated 36-72 hour; Get the secondary pure growth of photosynthetic bacterium; The photosynthetic bacterium secondary seed of getting 1% (volume ratio) is inoculated in fermentation propagation in the substratum of being made up of 2% (mass ratio) sodium acetate, 1% (mass ratio) ammonium chloride, 0.1% (mass ratio) potassium primary phosphate, 0.5% (mass ratio) sal epsom, 0.2 (mass ratio) sodium-chlor, 3% (mass ratio) amine molybdate, 0.01% (mass ratio) yeast extract, 0.05 (mass ratio) calcium chloride, 0.05% (mass ratio) iron(ic)chloride, 28-30 ℃ of static cultivation of illumination or not illumination shaking culture, liquid photosynthetic bacterium fermenting agent; Again liquid photosynthetic bacterium fermenting agent is inoculated on the solid medium, gets the bacterium number and reach 1.0-2.0 * 10
8The solid photosynthetic bacteria fermenting agent of individual/ml;
The cultivation of E, nitrobacteria: nitrobacteria comprises nitrococcus and nitrifier; Respectively the ammonia-state nitrogen in the water is converted into nitrite and nitrate salt; 24 ℃; Make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
Described nitrococcus substratum: ammonium sulfate
2SO
40.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, potassium hydrogenphosphate 1g, sal epsom 0.03g, calcium chloride 7.5g, zero(ppm) water 1000ml, PH nature, solid medium add 5% (mass ratio) agar;
Described nitrifier substratum: Sodium Nitrite 1g, sal epsom 0.03g, manganous sulfate 0.01g, potassium hydrogenphosphate 0.75g, yellow soda ash 1g, SODIUM PHOSPHATE, MONOBASIC N0.25g, zero(ppm) water 1000ml, PH nature, solid medium add 5% (mass ratio) agar;
F, subtilis, yeast, milk-acid bacteria, photosynthetic bacterium, nitrobacteria, bacillus licheniformis agent that above-mentioned A, B, C, D, E step are cultivated mix by certain mass ratio; Low temperature (≤60 ℃) drying; Moisture content is controlled at below 20%, just obtains complex microorganism preparations.
Embodiment 2:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it is processed by the raw material of following massfraction:
Preparation process is identical with embodiment 1.
Embodiment 3:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it is processed by the raw material of following massfraction:
Preparation process is identical with embodiment 1.
Embodiment 4:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it is processed by the raw material of following massfraction:
Preparation process is identical with embodiment 1.
Embodiment 5:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it is processed by the raw material of following massfraction:
Preparation process is identical with embodiment 1.
Embodiment 7:
A kind of complex microorganism preparations that is used for the aquaculture water water quality improvement, it is processed by the raw material of following massfraction:
Preparation process is identical with embodiment 1.
Test case 1:
2 mu of test water surfaces for aquaculture, 1.5 meters of the depth of waters, before using, water body is aging, and fish floating head phenomenon is general, and water transparency has only 15cm.Made the water edge red on June 20th, 2011, mu uses 3KG, with 100 times of clear water dilutions, evenly splashes in the pond, and after 7 days, transparency is brought up to 35cm; Dissolved oxygen rises to 1299mg/L from 772mg/L, and the Cyanophyta living weight drops to 14.832 mg/litre from 24.99 mg/litre; Macrozooplankton reduces relatively, and small-sized wheel animalcule obviously increases, and fish raises the nose above water to breathe phenomenon and disappears basically, water body be improved significantly.
Test case 2:
Test Jiangxia, Wuhan, place, 300 mu of water surfaces for aquaculture, depth of water 1.5-2 rice, before using, the water body algae is main with blue-green algae mainly mutually, blue-green algae quantity 32100 * 10
3Individual/L, water body is aging, and transparency is 20cm, and fish raises the nose above water to breathe in a large number; Made the water edge red on July 12nd, 2011, mu uses 4KG, with 100 times of clear water dilutions, evenly splashes in the pond, and after 7 days, transparency is brought up to 40cm; Detect water quality, blue-green algae quantity reduces to 8600 * 10
3Individual/L, other zooplanktons such as wheel animalcule, cladocera animal are increased, and the water body biological cycle is accelerated, and fish raises the nose above water to breathe phenomenon and disappears basically, water body be improved significantly.
Test case 3:
Test Wuhan, place Hong Shan, 220 mu of water surfaces for aquaculture, depth of water 2-3 rice, before using, body eutrophication is serious, and water body is aging, and transparency is 15cm, and fish raises the nose above water to breathe in a large number; Made the water edge red on July 25th, 2011, mu uses 3KG, with 100 times of clear water dilutions, evenly splashes in the pond, and after 7 days, transparency is brought up to 35cm; The water body biological cycle is accelerated, and fish raises the nose above water to breathe phenomenon and disappears basically, water body be improved significantly.
Claims (10)
1. complex microorganism preparations that is used for the aquaculture water water quality improvement, it is processed by the raw material of following massfraction:
Raw material weight per-cent
Subtilis 5%-50%
Yeast 1%-35%
Milk-acid bacteria 1%-30%
Photosynthetic bacterium 0.5%-30%
Nitrobacteria 0.1%-20%
Bacillus licheniformis 0.5%-25%.
2. a kind of complex microorganism preparations that is used for the aquaculture water water quality improvement according to claim 1, it is characterized in that: it is processed by the raw material of following massfraction:
Raw material weight per-cent
Subtilis 10%-45%
Yeast 5%-28%
Milk-acid bacteria 5%-25%
Photosynthetic bacterium 3%-25%
Nitrobacteria 4%-20%
Bacillus licheniformis 3%-18%.
3. a kind of complex microorganism preparations that is used for the aquaculture water water quality improvement according to claim 1, it is characterized in that: it is processed by the raw material of following massfraction:
Raw material weight per-cent
Subtilis 10%-35%
Yeast 8%-25%
Milk-acid bacteria 10%-20%
Photosynthetic bacterium 8%-20%
Nitrobacteria 10%-20%
Bacillus licheniformis 6%-15%.
4. a kind of complex microorganism preparations that is used for the aquaculture water water quality improvement according to claim 1, it is characterized in that: it is processed by the raw material of following massfraction:
Raw material weight per-cent
Subtilis 10%
Yeast 20%
Milk-acid bacteria 20%
Photosynthetic bacterium 20%
Nitrobacteria 20%
Bacillus licheniformis 10%.
5. a kind of complex microorganism preparations that is used for the aquaculture water water quality improvement according to claim 1, it is characterized in that: it is processed by the raw material of following massfraction:
Raw material weight per-cent
Subtilis 15%
Yeast 15%
Milk-acid bacteria 15%
Photosynthetic bacterium 20%
Nitrobacteria 20%
Bacillus licheniformis 15%.
6. a kind of complex microorganism preparations that is used for the aquaculture water water quality improvement according to claim 1, it is characterized in that: it is processed by the raw material of following massfraction:
Raw material weight per-cent
Subtilis 25%
Yeast 20%
Milk-acid bacteria 10%
Photosynthetic bacterium 20%
Nitrobacteria 10%
Bacillus licheniformis 15%.
7. a kind of complex microorganism preparations that is used for the aquaculture water water quality improvement according to claim 1, it is characterized in that: it is processed by the raw material of following massfraction:
Raw material weight per-cent
Subtilis 30%
Yeast 10%
Milk-acid bacteria 12%
Photosynthetic bacterium 18%
Nitrobacteria 20%
Bacillus licheniformis 10%.
8. the described a kind of preparation method who is used for the complex microorganism preparations of aquaculture water water quality improvement of claim 1 the steps include:
A, at first be the cultivation of subtilis, Bacillus licheniformis: subtilis, Bacillus licheniformis on beef-protein medium, 28-30
0C makes primary inclined plane and cultivates, and is inoculated into triangular flask then and does vibration secondary liquid culture, is inoculated at last to do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
B, secondly be saccharomycetic cultivation: yeast on lactic acid-yam-dextrose culture-medium, 28-30
0C makes primary inclined plane and cultivates, and is inoculated into then to do vibration secondary liquid culture in the triangular flask, is inoculated at last to do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
The cultivation of C, milk-acid bacteria: milk-acid bacteria on malt extract medium, 28-30
0C makes primary inclined plane and cultivates, and is inoculated into then to do vibration secondary liquid culture in the triangular flask, is inoculated at last to do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-5.0 * 10
8Individual/the gram solid medium;
The cultivation of D, photosynthetic bacterium: photosynthetic bacterium stoste is inoculated in the liquid test tube with cover 28-30
0C illumination cultivation 3-5 days, gets the photosynthetic bacterium bacteria suspension; Get the bacteria suspension of 1% volume ratio and put into the substratum of 500ml triangular flask deactivation, with Fan Shi substratum multiplication culture, constant-temperature shaking shaking table 28-30
0C; Cultivated 36-72 hour; Get the secondary pure growth of photosynthetic bacterium; The photosynthetic bacterium secondary seed of getting 1% volume ratio is inoculated in fermentation propagation in the substratum of being made up of than iron(ic)chloride than calcium chloride, 0.05% quality than yeast extract, 0.053% quality than amine molybdate, 0.01% quality than sodium-chlor, 3% quality than sal epsom, 0.23% quality than potassium primary phosphate, 0.5% quality than ammonium chloride, 0.1% quality than sodium acetate, 1% quality 2% quality, 28-30
0Static cultivation of C illumination or not illumination shaking culture get liquid photosynthetic bacterium fermenting agent, more liquid photosynthetic bacterium fermenting agent are inoculated on the solid medium, get the bacterium number and reach 1.0-2.0 * 10
8The solid photosynthetic bacteria fermenting agent of individual/ml;
The cultivation of E, nitrobacteria: nitrobacteria comprises nitrococcus and nitrifier; Respectively the ammonia-state nitrogen in the water is converted into nitrite and nitrate salt; 24 ℃; Make primary inclined plane and cultivate, be inoculated into then and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
F, subtilis, yeast, milk-acid bacteria, photosynthetic bacterium, nitrobacteria, bacillus licheniformis agent that above-mentioned A, B, C, D, E step are cultivated mix by mass ratio; Cryodrying; Moisture content is controlled at below 20%, just obtains complex microorganism preparations.
9. a kind of preparation method who is used for the complex microorganism preparations of aquaculture water water quality improvement according to claim 1 is characterized in that: described nitrococcus substratum: ammonium sulfate
2SO
40.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, potassium hydrogenphosphate 1g, sal epsom 0.03g, calcium chloride 7.5g, zero(ppm) water 1000ml, pH nature, solid medium add 5% quality and compare agar.
10. a kind of preparation method who is used for the complex microorganism preparations of aquaculture water water quality improvement according to claim 1 is characterized in that: described nitrifier substratum: Sodium Nitrite 1g, sal epsom 0.03g, manganous sulfate 0.01g, potassium hydrogenphosphate 0.75g, yellow soda ash 1g, SODIUM PHOSPHATE, MONOBASIC N0.25g, zero(ppm) water 1000ml, pH nature, solid medium add 5% quality and compare agar.
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Application publication date: 20120829 |