CN109729912A - A kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag - Google Patents
A kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag Download PDFInfo
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- CN109729912A CN109729912A CN201811440686.0A CN201811440686A CN109729912A CN 109729912 A CN109729912 A CN 109729912A CN 201811440686 A CN201811440686 A CN 201811440686A CN 109729912 A CN109729912 A CN 109729912A
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- 239000011669 selenium Substances 0.000 title claims abstract description 149
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 141
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 141
- 241000219000 Populus Species 0.000 title claims abstract description 139
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 91
- 230000003716 rejuvenation Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 124
- 238000002360 preparation method Methods 0.000 claims abstract description 23
- 241000894007 species Species 0.000 claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 claims abstract description 17
- 210000002686 mushroom body Anatomy 0.000 claims abstract description 7
- 229940091258 selenium supplement Drugs 0.000 claims description 134
- 235000015097 nutrients Nutrition 0.000 claims description 90
- 239000000843 powder Substances 0.000 claims description 70
- 235000013343 vitamin Nutrition 0.000 claims description 52
- 229940088594 vitamin Drugs 0.000 claims description 52
- 229930003231 vitamin Natural products 0.000 claims description 52
- 239000011782 vitamin Substances 0.000 claims description 52
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 52
- 244000062793 Sorghum vulgare Species 0.000 claims description 45
- 235000013312 flour Nutrition 0.000 claims description 45
- 235000019713 millet Nutrition 0.000 claims description 45
- 239000010440 gypsum Substances 0.000 claims description 40
- 229910052602 gypsum Inorganic materials 0.000 claims description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 30
- 238000011081 inoculation Methods 0.000 claims description 26
- 241000209094 Oryza Species 0.000 claims description 22
- 235000007164 Oryza sativa Nutrition 0.000 claims description 22
- 235000009566 rice Nutrition 0.000 claims description 22
- 239000000428 dust Substances 0.000 claims description 20
- 239000002023 wood Substances 0.000 claims description 20
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 15
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 15
- 238000012546 transfer Methods 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 12
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 12
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 12
- 244000251953 Agaricus brunnescens Species 0.000 claims description 10
- 229920001817 Agar Polymers 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 9
- 239000002689 soil Substances 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 238000011161 development Methods 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 5
- 238000004090 dissolution Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000002955 isolation Methods 0.000 claims description 5
- 235000013372 meat Nutrition 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 229960001471 sodium selenite Drugs 0.000 claims description 5
- 235000015921 sodium selenite Nutrition 0.000 claims description 5
- 239000011781 sodium selenite Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 5
- 235000009754 Vitis X bourquina Nutrition 0.000 claims 1
- 235000012333 Vitis X labruscana Nutrition 0.000 claims 1
- 240000006365 Vitis vinifera Species 0.000 claims 1
- 235000014787 Vitis vinifera Nutrition 0.000 claims 1
- 238000002156 mixing Methods 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 4
- 239000000796 flavoring agent Substances 0.000 abstract description 4
- 235000019634 flavors Nutrition 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 241000233866 Fungi Species 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 238000011534 incubation Methods 0.000 abstract description 2
- 239000013587 production medium Substances 0.000 abstract description 2
- 238000012549 training Methods 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000008520 organization Effects 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 235000007686 potassium Nutrition 0.000 description 3
- 206010039921 Selenium deficiency Diseases 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 230000003026 anti-oxygenic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000001497 healthy food Nutrition 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
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- 239000000575 pesticide Substances 0.000 description 1
- 150000003346 selenoethers Chemical class 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention relates to edible fungus species technical fields, especially a kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag, including S1 kind mushroom selection, S2 tissue be separately cultured, S3 preparation parent species, S4 preparation level-one kind, the culture of S5 first time tube, second of tube culture of S6, the culture of S7 third time tube, the 4th tube culture of S8, S9 extension original seed, S10 preparation production kind and etc..The present invention is kind of a mushroom with the wild excellent mushroom mushroom body of the trees association in selenium-rich mountain area in breeding, and parent species are extracted, in the multiple rejuvenation incubation of parent species, utilize the continuous variation of culture medium, to improve the adaptability of selenium-rich mushroom strain and production medium, it realizes the rejuvenation of wild selenium-rich mushroom strain, improves strain to the absorption rate of selenium fertilizer, the mushroom selenium content of production, flavor, quality are high, it is with strong points, pollution scrappage is low, and mushroom high conversion rate is good in economic efficiency.
Description
Technical field
The present invention relates to edible fungus species technical field, the wild selenium-rich of especially a kind of poplar sawdust cultivating in bag is fragrant
The rejuvenation method of mushroom strains.
Background technique
Mushroom production occupies second in world artificial cultivation edible mushroom.With the raising of quality of the life, people start by
The nutritive value for gradually recognizing mushroom becomes people and most likes due to the effect of its unique flavor and enhancing immune function of human body
One of healthy food of love.
In recent years, since the supply in wild-type champignon market is not asked, mushroom is grown naturally from field gradually becomes people
Work domesticating and cultivating, therefore the yield of mushroom is greatly improved, it is in order to more meet ever-increasing market demand, then more next
More farmers is in order to mention the various chemical fertilizer of throughput applications and pesticide, although so that mushroom production increases mushroom wind
Taste differs farther out compared with wild-type champignon, and as people constantly pursue healthy living theory, vast consumer more favors
In wild-type champignon, therefore wild-type champignon resource is seriously damaged on a large scale.
Selenium is the necessary microelement of human body, selenium and selenides in addition to antioxygenic property, also have it is antitumor, prolong
The functions such as long-life and raising immunity of organisms.There is selenium deficiency in more than 40 country's soil in the world, China has 70% area to belong to
Selenium-rich product is found and is developed in selenium deficiency area, just of interest by various countries scientific worker to guarantee the sufficient selenium of human body intake.
Currently, being in research using edible mushroom as the carrier of enrichment selenium element in China.Especially cultivating champignon
Area is wide, easy to operate, and accumulation ability is significant, and using mushroom as the carrier of enrichment selenium element, researcher is more.And current
The research of Se-rich xianggu, in terms of the plantation for being concentrated mainly on Se-rich xianggu, in terms of taming rejuvenation to wild selenium-rich mushroom strain
It studies less.Although the mushroom selenium content researched and produced simultaneously is higher, the flavor of mushroom, inferior quality；Turn in production
In inoculation, mushroom strain degenerate problem is serious, relatively low to the absorption rate of selenium fertilizer, is subject to pollute, resistance is poor, mushroom
Conversion ratio is low, and the economic benefit is not high by farmer.
Summary of the invention
The purpose of the present invention is to provide a kind of rejuvenation sides of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag
Method, to solve the problems mentioned in the above background technology.
To achieve the goals above, the invention provides the following technical scheme:
A kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag, comprising the following steps:
S1, kind mushroom selection: in 6~15 DEG C of winter-spring season, the trees in Anhui Shitai County mountain area containing selenium-rich soil are chosen at
The development stalwartness of association, cap Gai Hou great, no disease and pests harm, maturity wild-type champignon mushroom body appropriate are kind of a mushroom, and are packed into sterile
It is taken back in paper bag；
S2, tissue are separately cultured: by the kind mushroom of selection, separating kind of mushroom canopy and stem intersection using tissue isolation
Fritter meat bacteria organization is placed in the basic PDA culture medium in culture dish, and culture a period of time simultaneously removes the training polluted in time
Support object；
S3, preparation parent species: the excellent tissue cultures strain of character obtained in previous step is selected with transfer needle, is placed in
Leaf sap containing poplar, Selenium-rich nutrient solution in test tube, basic PDA culture medium mixed culture medium on, move into 18~22 DEG C again
Insulating box in, regular bevel culture 10~15 days, be finally placed in ultralow temperature -80~-50 DEG C preservation, it is fragrant that wild selenium-rich be made
Mushroom parent species；By percentage to the quality, the mixed culture medium, component include: poplar leaf sap 1.0%~3.0%, selenium-rich
Nutrient solution 0.5%~0.8%, surplus are basic PDA culture medium；
S4, preparation level-one kind: wild Se-rich xianggu parent species obtained in previous step are selected with transfer needle, with Poplar leaves
Juice, Selenium-rich nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15
It, is made level-one kind；By percentage to the quality, the poplar leaf sap, Selenium-rich nutrient solution, millet flour, basic PDA culture medium
Mixture, component includes: poplar leaf sap 3.0%~5.0%, Selenium-rich nutrient solution 0.8%~1.2%, millet flour
1.0%~3.0%, surplus is basic PDA culture medium；
S5, first time tube culture: level-one kind obtained in previous step is selected with transfer needle, with poplar leaf sap, richness
Selenium nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15 days；
S6, second of tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap,
Poplar leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, potassium dihydrogen phosphate, magnesium sulfate, vitamin, water are culture medium, are connect
Kind, regular bevel culture 10~15 days；
S7, third time tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap,
Poplar leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, gypsum, vitamin, water are culture medium, inoculation, regular bevel culture
10~15 days；
S8, the 4th tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf powder,
Poplar wood dust, Selenium-rich nutrient solution, bran powder, gypsum, vitamin, water are culture medium, inoculation, regular bevel culture 10~15
It；
S9, extension original seed: it using inclined-plane culture mycelia obtained in previous step as strain, is sought with poplar wood dust, selenium-rich
Nutrient solution, bran powder, rice bran, gypsum, vitamin, water are culture medium, inoculation, wide-mouth bottle routine culture, until mycelia sends out in wide-mouth bottle
Until full；
S10, preparation production kind: selecting step S9 culture mushroom strain, with poplar sawdust, Selenium-rich nutrient solution, rice bran,
Gypsum, vitamin, water are culture medium, and pack, is inoculated with, routine culture sterilizing, until mycelia hair in bacterium bag is full, are obtained
It can be applied to the wild selenium-rich mushroom strain of the rejuvenation of poplar sawdust cultivating in bag mass production.
Preferably, in S5 step, by percentage to the quality, the culture medium, component includes: poplar leaf sap
5.0%~8.0%, Selenium-rich nutrient solution 1.2%~1.5%, millet flour 3.0%~5.0%, surplus are basic PDA culture medium.
Preferably, in S6 step, by percentage to the quality, the culture medium, component includes: poplar leaf sap
8.0%~10.0%, poplar leaf powder 3.0%~5.0%, Selenium-rich nutrient solution 1.5%~1.8%, millet flour 5.0%~
8.0%, bran powder 2.0%~4.0%, surplus is potassium dihydrogen phosphate, magnesium sulfate, vitamin and water.
Preferably, in S7 step, by percentage to the quality, the culture medium, component includes: poplar leaf sap
8.0%~10.0%, poplar leaf powder 5.0%~8.0%, Selenium-rich nutrient solution 1.8%~2.2%, millet flour 5.0%~
8.0%, bran powder 4.0%~8.0%, surplus is gypsum, vitamin, He Shui.
Preferably, in S8 step, by percentage to the quality, the culture medium, component includes: poplar leaf powder
10.0%~15.0%, poplar wood dust 8.0%~15.0%, Selenium-rich nutrient solution 2.2%~2.5%, bran powder 8.0%~
12.0%, surplus is gypsum, vitamin, He Shui.
Preferably, in S9 step, by percentage to the quality, the culture medium, component includes: poplar wood dust
18.0%~30.0%, Selenium-rich nutrient solution 2.5%~3.5%, bran powder 4.0%~8.0%, rice bran 3.0%~7.0% are remaining
Amount is gypsum, vitamin, He Shui.
Preferably, in S10 step, by percentage to the quality, the culture medium, component includes: poplar sawdust
30.0%~50.0%, Selenium-rich nutrient solution 3.5%~5.0%, rice bran 10.0%~20.0%, surplus be gypsum, vitamin,
And water.
Preferably, the basic PDA culture medium, preparation method are as follows: the potato fritter 200g for cleaning peeling is weighed,
It is cut into small pieces, adds water 1000ml, boil after twenty minutes, filtering supplies moisture to 1000ml in filtered juice；It is separately added into fine jade again
Rouge 18g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, vitamin 10mg, after boiling dissolution, supply moisture；Finally
Packing sterilizes, is spare, obtains base agar culture medium.
Preferably, the Selenium-rich nutrient solution is sodium selenite solution.
The beneficial effects of the present invention are:
A kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag of the present invention, in breeding, with richness
The wild excellent mushroom mushroom body of the trees association in selenium mountain area is kind of a mushroom, and extracts parent species, in the multiple rejuvenation incubation of parent species
In, using the continuous variation of culture medium, the adaptability of Lai Tigao selenium-rich mushroom strain and production medium is realized and is directed to poplar
The rejuvenation of the mushroom strain of sawdust cultivating in bag production, mushroom strain in raising to the absorption rate of selenium fertilizer, production
Mushroom selenium content, flavor, quality are high, with strong points, and pollution scrappage is low, and mushroom high conversion rate is good in economic efficiency, have real
Border directive significance and application value.
Specific embodiment
In order to facilitate the understanding of those skilled in the art, the present invention is further illustrated combined with specific embodiments below.
Embodiment 1:
A kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag, comprising the following steps:
S1, kind mushroom selection: in 6~15 DEG C of winter-spring season, the trees in Anhui Shitai County mountain area containing selenium-rich soil are chosen at
The development stalwartness of association, cap Gai Hou great, no disease and pests harm, maturity wild-type champignon mushroom body appropriate are kind of a mushroom, and are packed into sterile
It is taken back in paper bag；
S2, tissue are separately cultured: by the kind mushroom of selection, separating kind of mushroom canopy and stem intersection using tissue isolation
Fritter meat bacteria organization is placed in the basic PDA culture medium in culture dish, and culture a period of time simultaneously removes the training polluted in time
Support object；
S3, preparation parent species: the excellent tissue cultures strain of character obtained in previous step is selected with transfer needle, is placed in
Leaf sap containing poplar, Selenium-rich nutrient solution in test tube, basic PDA culture medium mixed culture medium on, move into 18~22 DEG C again
Insulating box in, regular bevel culture 10~15 days, be finally placed in ultralow temperature -80~-50 DEG C preservation, it is fragrant that wild selenium-rich be made
Mushroom parent species；By percentage to the quality, the mixed culture medium, component include: poplar leaf sap 1.0%, Selenium-rich nutrient solution
0.5%, surplus is basic PDA culture medium；
S4, preparation level-one kind: wild Se-rich xianggu parent species obtained in previous step are selected with transfer needle, with Poplar leaves
Juice, Selenium-rich nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15
It, is made level-one kind；By percentage to the quality, the poplar leaf sap, Selenium-rich nutrient solution, millet flour, basic PDA culture medium
Mixture, based on component includes: poplar leaf sap 3.0%, Selenium-rich nutrient solution 0.8%, millet flour 3.0%, surplus
PDA culture medium；
S5, first time tube culture: level-one kind obtained in previous step is selected with transfer needle, with poplar leaf sap, richness
Selenium nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15 days；With matter
Percentages are measured, the culture medium, component includes: poplar leaf sap 5.0%, Selenium-rich nutrient solution 1.2%, millet flour
5.0%, surplus is basic PDA culture medium.
S6, second of tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap,
Poplar leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, potassium dihydrogen phosphate, magnesium sulfate, vitamin, water are culture medium, are connect
Kind, regular bevel culture 10~15 days；By percentage to the quality, the culture medium, component include: poplar leaf sap
8.0%, poplar leaf powder 5.0%, Selenium-rich nutrient solution 1.5%, millet flour 8.0%, bran powder 2.0%, surplus is di(2-ethylhexyl)phosphate
Hydrogen potassium, magnesium sulfate, vitamin and water.
S7, third time tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap,
Poplar leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, gypsum, vitamin, water are culture medium, inoculation, regular bevel culture
10~15 days；By percentage to the quality, the culture medium, component include: poplar leaf sap 8.0%, poplar leaf powder
8.0%, Selenium-rich nutrient solution 1.8%, millet flour 5.0%, bran powder 8.0%, surplus is gypsum, vitamin, He Shui.
S8, the 4th tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf powder,
Poplar wood dust, Selenium-rich nutrient solution, bran powder, gypsum, vitamin, water are culture medium, inoculation, regular bevel culture 10~15
It；By percentage to the quality, the culture medium, component include: poplar leaf powder 10.0%, and poplar wood dust 8.0% is rich
Selenium nutrient solution 2.2%, bran powder 12.0%, surplus are gypsum, vitamin, He Shui.
S9, extension original seed: it using inclined-plane culture mycelia obtained in previous step as strain, is sought with poplar wood dust, selenium-rich
Nutrient solution, bran powder, rice bran, gypsum, vitamin, water are culture medium, inoculation, wide-mouth bottle routine culture, until mycelia sends out in wide-mouth bottle
Until full；By percentage to the quality, the culture medium, component include: poplar wood dust 18.0%, Selenium-rich nutrient solution
2.5%, bran powder 4.0%, rice bran 7.0%, surplus are gypsum, vitamin, He Shui.
S10, preparation production kind: selecting step S9 culture mushroom strain, with poplar sawdust, Selenium-rich nutrient solution, rice bran,
Gypsum, vitamin, water are culture medium, and pack, is inoculated with, routine culture sterilizing, until mycelia hair in bacterium bag is full, are obtained
It can be applied to the wild selenium-rich mushroom strain of the rejuvenation of poplar sawdust cultivating in bag mass production.By percentage to the quality, the training
Base is supported, component includes: poplar sawdust 30.0%, and Selenium-rich nutrient solution 3.5%, rice bran 20.0%, surplus is gypsum, dimension life
Element and water.
Wherein, basic PDA culture medium described above, preparation method are as follows: weigh the potato fritter for cleaning peeling
200g is cut into small pieces, and adds water 1000ml, boils after twenty minutes, and filtering supplies moisture to 1000ml in filtered juice；Add respectively again
Enter agar 18g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, vitamin 10mg, after boiling dissolution, supplies moisture；
It finally dispenses, sterilizes, is spare, obtain base agar culture medium.
Wherein, Selenium-rich nutrient solution described above is sodium selenite solution.
Embodiment 2:
A kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag, comprising the following steps:
S1, kind mushroom selection: in 6~15 DEG C of winter-spring season, the trees in Anhui Shitai County mountain area containing selenium-rich soil are chosen at
The development stalwartness of association, cap Gai Hou great, no disease and pests harm, maturity wild-type champignon mushroom body appropriate are kind of a mushroom, and are packed into sterile
It is taken back in paper bag；
S2, tissue are separately cultured: by the kind mushroom of selection, separating kind of mushroom canopy and stem intersection using tissue isolation
Fritter meat bacteria organization is placed in the basic PDA culture medium in culture dish, and culture a period of time simultaneously removes the training polluted in time
Support object；
S3, preparation parent species: the excellent tissue cultures strain of character obtained in previous step is selected with transfer needle, is placed in
Leaf sap containing poplar, Selenium-rich nutrient solution in test tube, basic PDA culture medium mixed culture medium on, move into 18~22 DEG C again
Insulating box in, regular bevel culture 10~15 days, be finally placed in ultralow temperature -80~-50 DEG C preservation, it is fragrant that wild selenium-rich be made
Mushroom parent species；By percentage to the quality, the mixed culture medium, component include: poplar leaf sap 3.0%, Selenium-rich nutrient solution
0.8%, surplus is basic PDA culture medium；
S4, preparation level-one kind: wild Se-rich xianggu parent species obtained in previous step are selected with transfer needle, with Poplar leaves
Juice, Selenium-rich nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15
It, is made level-one kind；By percentage to the quality, the poplar leaf sap, Selenium-rich nutrient solution, millet flour, basic PDA culture medium
Mixture, based on component includes: poplar leaf sap 5.0%, Selenium-rich nutrient solution 1.2%, millet flour 1.0%, surplus
PDA culture medium；
S5, first time tube culture: level-one kind obtained in previous step is selected with transfer needle, with poplar leaf sap, richness
Selenium nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15 days；With matter
Percentages are measured, the culture medium, component includes: poplar leaf sap 8.0%, Selenium-rich nutrient solution 1.5%, millet flour
3.0%, surplus is basic PDA culture medium.
S6, second of tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap,
Poplar leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, potassium dihydrogen phosphate, magnesium sulfate, vitamin, water are culture medium, are connect
Kind, regular bevel culture 10~15 days；By percentage to the quality, the culture medium, component include: poplar leaf sap
10.0%, poplar leaf powder 3.0%, Selenium-rich nutrient solution 1.8%, millet flour 5.0%, bran powder 4.0%, surplus is di(2-ethylhexyl)phosphate
Hydrogen potassium, magnesium sulfate, vitamin and water.
S7, third time tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap,
Poplar leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, gypsum, vitamin, water are culture medium, inoculation, regular bevel culture
10~15 days；By percentage to the quality, the culture medium, component include: poplar leaf sap 10.0%, poplar leaf powder
5.0%, Selenium-rich nutrient solution 2.2%, millet flour 8.0%, bran powder 4.0%, surplus is gypsum, vitamin, He Shui.
S8, the 4th tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf powder,
Poplar wood dust, Selenium-rich nutrient solution, bran powder, gypsum, vitamin, water are culture medium, inoculation, regular bevel culture 10~15
It；By percentage to the quality, the culture medium, component include: poplar leaf powder 15.0%, and poplar wood dust 15.0% is rich
Selenium nutrient solution 2.5%, bran powder 8.0%, surplus are gypsum, vitamin, He Shui.
S9, extension original seed: it using inclined-plane culture mycelia obtained in previous step as strain, is sought with poplar wood dust, selenium-rich
Nutrient solution, bran powder, rice bran, gypsum, vitamin, water are culture medium, inoculation, wide-mouth bottle routine culture, until mycelia sends out in wide-mouth bottle
Until full；By percentage to the quality, the culture medium, component include: poplar wood dust 30.0%, Selenium-rich nutrient solution
3.5%, bran powder 8.0%, rice bran 3.0%, surplus are gypsum, vitamin, He Shui.
S10, preparation production kind: selecting step S9 culture mushroom strain, with poplar sawdust, Selenium-rich nutrient solution, rice bran,
Gypsum, vitamin, water are culture medium, and pack, is inoculated with, routine culture sterilizing, until mycelia hair in bacterium bag is full, are obtained
It can be applied to the wild selenium-rich mushroom strain of the rejuvenation of poplar sawdust cultivating in bag mass production.By percentage to the quality, the training
Base is supported, component includes: poplar sawdust 50.0%, and Selenium-rich nutrient solution 5.0%, rice bran 10.0%, surplus is gypsum, dimension life
Element and water.
Wherein, basic PDA culture medium described above, preparation method are as follows: weigh the potato fritter for cleaning peeling
200g is cut into small pieces, and adds water 1000ml, boils after twenty minutes, and filtering supplies moisture to 1000ml in filtered juice；Add respectively again
Enter agar 18g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, vitamin 10mg, after boiling dissolution, supplies moisture；
It finally dispenses, sterilizes, is spare, obtain base agar culture medium.
Wherein, Selenium-rich nutrient solution described above is sodium selenite solution.
Embodiment 3:
A kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag, comprising the following steps:
S1, kind mushroom selection: in 6~15 DEG C of winter-spring season, the trees in Anhui Shitai County mountain area containing selenium-rich soil are chosen at
The development stalwartness of association, cap Gai Hou great, no disease and pests harm, maturity wild-type champignon mushroom body appropriate are kind of a mushroom, and are packed into sterile
It is taken back in paper bag；
S2, tissue are separately cultured: by the kind mushroom of selection, separating kind of mushroom canopy and stem intersection using tissue isolation
Fritter meat bacteria organization is placed in the basic PDA culture medium in culture dish, and culture a period of time simultaneously removes the training polluted in time
Support object；
S3, preparation parent species: the excellent tissue cultures strain of character obtained in previous step is selected with transfer needle, is placed in
Leaf sap containing poplar, Selenium-rich nutrient solution in test tube, basic PDA culture medium mixed culture medium on, move into 18~22 DEG C again
Insulating box in, regular bevel culture 10~15 days, be finally placed in ultralow temperature -80~-50 DEG C preservation, it is fragrant that wild selenium-rich be made
Mushroom parent species；By percentage to the quality, the mixed culture medium, component include: poplar leaf sap 2.0%, Selenium-rich nutrient solution
0.65%, surplus is basic PDA culture medium；
S4, preparation level-one kind: wild Se-rich xianggu parent species obtained in previous step are selected with transfer needle, with Poplar leaves
Juice, Selenium-rich nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15
It, is made level-one kind；By percentage to the quality, the poplar leaf sap, Selenium-rich nutrient solution, millet flour, basic PDA culture medium
Mixture, based on component includes: poplar leaf sap 4.0%, Selenium-rich nutrient solution 1.0%, millet flour 2.0%, surplus
PDA culture medium；
S5, first time tube culture: level-one kind obtained in previous step is selected with transfer needle, with poplar leaf sap, richness
Selenium nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15 days；With matter
Percentages are measured, the culture medium, component includes: poplar leaf sap 6.5%, Selenium-rich nutrient solution 1.35%, millet flour
4.0%, surplus is basic PDA culture medium.
S6, second of tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap,
Poplar leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, potassium dihydrogen phosphate, magnesium sulfate, vitamin, water are culture medium, are connect
Kind, regular bevel culture 10~15 days；By percentage to the quality, the culture medium, component include: poplar leaf sap
9.0%, poplar leaf powder 4.0%, Selenium-rich nutrient solution 1.65%, millet flour 6.5%, bran powder 3.0%, surplus is di(2-ethylhexyl)phosphate
Hydrogen potassium, magnesium sulfate, vitamin and water.
S7, third time tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap,
Poplar leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, gypsum, vitamin, water are culture medium, inoculation, regular bevel culture
10~15 days；By percentage to the quality, the culture medium, component include: poplar leaf sap 9.0%, poplar leaf powder
6.5%, Selenium-rich nutrient solution 2.0%, millet flour 6.5%, bran powder 6.0%, surplus is gypsum, vitamin, He Shui.
S8, the 4th tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf powder,
Poplar wood dust, Selenium-rich nutrient solution, bran powder, gypsum, vitamin, water are culture medium, inoculation, regular bevel culture 10~15
It；By percentage to the quality, the culture medium, component include: poplar leaf powder 12.5%, and poplar wood dust 12.0% is rich
Selenium nutrient solution 2.35%, bran powder 10.0%, surplus are gypsum, vitamin, He Shui.
S9, extension original seed: it using inclined-plane culture mycelia obtained in previous step as strain, is sought with poplar wood dust, selenium-rich
Nutrient solution, bran powder, rice bran, gypsum, vitamin, water are culture medium, inoculation, wide-mouth bottle routine culture, until mycelia sends out in wide-mouth bottle
Until full；By percentage to the quality, the culture medium, component include: poplar wood dust 24.0%, Selenium-rich nutrient solution
3.0%, bran powder 6.0%, rice bran 5.0%, surplus are gypsum, vitamin, He Shui.
S10, preparation production kind: selecting step S9 culture mushroom strain, with poplar sawdust, Selenium-rich nutrient solution, rice bran,
Gypsum, vitamin, water are culture medium, and pack, is inoculated with, routine culture sterilizing, until mycelia hair in bacterium bag is full, are obtained
It can be applied to the wild selenium-rich mushroom strain of the rejuvenation of poplar sawdust cultivating in bag mass production.By percentage to the quality, the training
Base is supported, component includes: poplar sawdust 40.0%, and Selenium-rich nutrient solution 4.2%, rice bran 15.0%, surplus is gypsum, dimension life
Element and water.
Wherein, basic PDA culture medium described above, preparation method are as follows: weigh the potato fritter for cleaning peeling
200g is cut into small pieces, and adds water 1000ml, boils after twenty minutes, and filtering supplies moisture to 1000ml in filtered juice；Add respectively again
Enter agar 18g, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, vitamin 10mg, after boiling dissolution, supplies moisture；
It finally dispenses, sterilizes, is spare, obtain base agar culture medium.
Wherein, Selenium-rich nutrient solution described above is sodium selenite solution.
It is above-mentioned that invention is exemplarily described, it is clear that present invention specific implementation is not subject to the restrictions described above, only
To use this insubstantial improvement that the inventive concept and technical scheme of the present invention carry out, or the not improved structure by invention
Think and technical solution directly applies to other occasions, it is within the scope of the present invention.
Claims (9)
1. a kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag, which is characterized in that including following step
It is rapid:
S1, kind mushroom selection: in 6~15 DEG C of winter-spring season, it is chosen at the trees association in Anhui Shitai County mountain area containing selenium-rich soil
Development stalwartness, cap Gai Hou great, no disease and pests harm, maturity wild-type champignon mushroom body appropriate be kind of a mushroom, and be packed into sterile paper bag
Inside take back；
S2, tissue are separately cultured: by the kind mushroom of selection, separating kind of mushroom canopy and the small ferfas of stem intersection using tissue isolation
Meat tissue is placed in the basic PDA culture medium in culture dish, and culture a period of time simultaneously removes the culture polluted in time；
S3, preparation parent species: the excellent tissue cultures strain of character obtained in previous step is selected with transfer needle, is placed in test tube
Leaf sap containing poplar, Selenium-rich nutrient solution, basic PDA culture medium mixed culture medium on, move into 18~22 DEG C of constant temperature again
In case, regular bevel culture 10~15 days, it is finally placed in ultralow temperature -80~-50 DEG C preservation, wild Se-rich xianggu parent species are made；
By percentage to the quality, the mixed culture medium, component include: poplar leaf sap 1.0%~3.0%, Selenium-rich nutrient solution
0.5%~0.8%, surplus is basic PDA culture medium；
S4, preparation level-one kind: select wild Se-rich xianggu parent species obtained in previous step with transfer needle, with poplar leaf sap,
Selenium-rich nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15 days, system
Obtain level-one kind；By percentage to the quality, the mixing of the poplar leaf sap, Selenium-rich nutrient solution, millet flour, basic PDA culture medium
Object, component include: poplar leaf sap 3.0%~5.0%, Selenium-rich nutrient solution 0.8%~1.2%, millet flour 1.0%~
3.0%, surplus is basic PDA culture medium；
S5, first time tube culture: selecting level-one kind obtained in previous step with transfer needle, is sought with poplar leaf sap, selenium-rich
Nutrient solution, millet flour, basic PDA culture medium mixture be culture medium, inoculation, regular bevel culture 10~15 days；
S6, second of tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap, poplar
Leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, potassium dihydrogen phosphate, magnesium sulfate, vitamin, water are culture medium, and inoculation is conventional
Inclined-plane culture 10~15 days；
S7, third time tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf sap, poplar
Leaf powder, Selenium-rich nutrient solution, millet flour, bran powder, gypsum, vitamin, water are culture medium, are inoculated with, regular bevel culture 10~
15 days；
S8, the 4th tube culture: using inclined-plane culture mycelia obtained in previous step as strain, with poplar leaf powder, poplar
Wood dust, Selenium-rich nutrient solution, bran powder, gypsum, vitamin, water are culture medium, are inoculated with, regular bevel culture 10~15 days；
S9, extension original seed: using inclined-plane culture mycelia obtained in previous step as strain, with poplar wood dust, Selenium-rich nutrient solution,
Bran powder, rice bran, gypsum, vitamin, water are culture medium, inoculation, wide-mouth bottle routine culture, until mycelia hair is completely in wide-mouth bottle
Only；
S10, preparation production kind: selecting step S9 culture mushroom strain, with poplar sawdust, Selenium-rich nutrient solution, rice bran, gypsum,
Vitamin, water are culture medium, and pack, sterilizing, inoculation, routine culture, until mycelia hair in bacterium bag is full, obtaining can be applied
In the wild selenium-rich mushroom strain of the rejuvenation of poplar sawdust cultivating in bag mass production.
2. a kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag according to claim 1, special
Sign is, in S5 step, by percentage to the quality, the culture medium, component include: poplar leaf sap 5.0%~
8.0%, Selenium-rich nutrient solution 1.2%~1.5%, millet flour 3.0%~5.0%, surplus are basic PDA culture medium.
3. a kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag according to claim 1, special
Sign is, in S6 step, by percentage to the quality, the culture medium, component include: poplar leaf sap 8.0%~
10.0%, poplar leaf powder 3.0%~5.0%, Selenium-rich nutrient solution 1.5%~1.8%, millet flour 5.0%~8.0%, rice bran
Powder 2.0%~4.0%, surplus are potassium dihydrogen phosphate, magnesium sulfate, vitamin and water.
4. a kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag according to claim 1, special
Sign is, in S7 step, by percentage to the quality, the culture medium, component include: poplar leaf sap 8.0%~
10.0%, poplar leaf powder 5.0%~8.0%, Selenium-rich nutrient solution 1.8%~2.2%, millet flour 5.0%~8.0%, rice bran
Powder 4.0%~8.0%, surplus are gypsum, vitamin, He Shui.
5. a kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag according to claim 1, special
Sign is, in S8 step, by percentage to the quality, the culture medium, component include: poplar leaf powder 10.0%~
15.0%, poplar wood dust 8.0%~15.0%, Selenium-rich nutrient solution 2.2%~2.5%, bran powder 8.0%~12.0% is remaining
Amount is gypsum, vitamin, He Shui.
6. a kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag according to claim 1, special
Sign is, in S9 step, by percentage to the quality, the culture medium, component include: poplar wood dust 18.0%~
30.0%, Selenium-rich nutrient solution 2.5%~3.5%, bran powder 4.0%~8.0%, rice bran 3.0%~7.0%, surplus be gypsum,
Vitamin, He Shui.
7. a kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag according to claim 1, special
Sign is, in S10 step, by percentage to the quality, the culture medium, component include: poplar sawdust 30.0%~
50.0%, Selenium-rich nutrient solution 3.5%~5.0%, rice bran 10.0%~20.0%, surplus is gypsum, vitamin, He Shui.
8. a kind of rejuvenation method of the wild selenium-rich mushroom strain of poplar sawdust cultivating in bag according to claim 1, special
Sign is, the basis PDA culture medium, preparation method are as follows: the potato fritter 200g for cleaning peeling is weighed, is cut into small pieces,
Add water 1000ml, boil after twenty minutes, filtering supplies moisture to 1000ml in filtered juice；It is separately added into agar 18g, grape again
Sugared 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, vitamin 10mg, after boiling dissolution, supply moisture；It finally dispenses, sterilizes, is standby
With obtaining base agar culture medium.
9. a kind of acclimation method of wild selenium-rich mushroom strain described in -7 any one according to claim 1, which is characterized in that
The Selenium-rich nutrient solution is sodium selenite solution.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004068A (en) * | 2019-05-13 | 2019-07-12 | 江苏春之雨生物科技发展有限公司 | A kind of mushroom strain storage medium and method for preserving |
| CN113528354A (en) * | 2021-07-19 | 2021-10-22 | 上海市农业科学院 | Lentinula edodes monokaryon culture medium, and preparation method and application thereof |
| CN115885769A (en) * | 2022-11-16 | 2023-04-04 | 重庆华绿生物有限公司 | A kind of cultivation method of Flammulina velutipes bacterial classification |
-
2018
- 2018-11-29 CN CN201811440686.0A patent/CN109729912A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004068A (en) * | 2019-05-13 | 2019-07-12 | 江苏春之雨生物科技发展有限公司 | A kind of mushroom strain storage medium and method for preserving |
| CN110004068B (en) * | 2019-05-13 | 2023-06-30 | 江苏春之雨生物科技发展有限公司 | Lentinus edodes strain preservation culture medium and preservation method |
| CN113528354A (en) * | 2021-07-19 | 2021-10-22 | 上海市农业科学院 | Lentinula edodes monokaryon culture medium, and preparation method and application thereof |
| CN115885769A (en) * | 2022-11-16 | 2023-04-04 | 重庆华绿生物有限公司 | A kind of cultivation method of Flammulina velutipes bacterial classification |
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