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CN109302954A - A method for in situ restoration of saline-alkali land ecosystem in the Yellow Triangle using Nandi and biochar - Google Patents

A method for in situ restoration of saline-alkali land ecosystem in the Yellow Triangle using Nandi and biochar Download PDF

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Publication number
CN109302954A
CN109302954A CN201811397399.6A CN201811397399A CN109302954A CN 109302954 A CN109302954 A CN 109302954A CN 201811397399 A CN201811397399 A CN 201811397399A CN 109302954 A CN109302954 A CN 109302954A
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soil
biochar
saline
miscanthus
water
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周功克
何康
胡瑞波
贺郭
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Qingdao Institute of Bioenergy and Bioprocess Technology of CAS
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Qingdao Institute of Bioenergy and Bioprocess Technology of CAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Mechanical Engineering (AREA)
  • Wood Science & Technology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

本发明公开了利用南荻及生物炭原位修复黄三角盐碱地生态系统的方法,属于盐碱地改良技术领域,生物炭添加具体为:根据土壤酸碱度、含盐量以及土壤性质计算,黄三角滨海盐碱地的土壤pH8.5,含盐量0.75%,土壤性质为沙质黏壤土,有机质含量低,生物炭施用量土壤重量的2%‑4%;施用方法:(1)平整土地,(2)按照改良地点土壤密度以及种植深度计算生物炭具体添加量,生物炭的具体添加量为土壤重量的2‑4%,土壤重量的计算方法为土壤重量=改良地点的表面积*种植深度*土壤密度;(3)将计算好的生物炭均匀铺在土壤表面,翻土,拌匀;(4)拌匀后熟化三天,即可种植南荻芒草;本发明技术方案能够对黄河三角洲滨海地区的盐碱地生态系统进行原位修复,修复后的盐碱地土壤理化特征和生物群落结构发生很大变化,能够适宜芒草存活并正常生长。

The invention discloses a method for in-situ restoration of the saline-alkali land ecosystem in the Yellow Delta by utilizing Nandi and biochar, and belongs to the technical field of saline-alkali land improvement. pH8.5, salt content 0.75%, soil properties are sandy clay loam, low organic matter content, biochar application rate is 2%-4% of soil weight; application method: (1) leveling the land, (2) according to the improvement site The specific amount of biochar added is calculated from soil density and planting depth. The specific amount of biochar added is 2-4% of the soil weight. The calculation method of soil weight is soil weight = surface area of the improvement site * planting depth * soil density; (3) Evenly spread the calculated biochar on the soil surface, turn the soil, and mix well; (4) after being mixed well and mature for three days, Miscanthus can be planted; After site restoration, the physicochemical characteristics and biological community structure of the restored saline-alkali soil changed greatly, which was suitable for Miscanthus to survive and grow normally.

Description

Utilize southern reed and the method for charcoal in-situ immobilization Huang triangle saline field ecosystem
Technical field
The invention belongs to ecology of alkali saline land to repair field, more particularly to a kind of yellow using southern reed and charcoal in-situ immobilization The method of triangle saline field ecosystem.
Background technique
The salinization of soil is a global problem.China is third place in the world big salt-soda soil distribution country.The Huanghe delta Be land-sea interaction formed move back Haiti, annual rainfall is few, and evaporation capacity is big, and soil salinization phenomenon is very general in the region Time.According to soil salt content, salt-soda soil can be divided into slight, moderate, heavy salinized, and general slight alkaline land soil salt content is 0.1%~0.3%, moderate is 0.3%~0.6%, and severe is > 0.6%.According to statistics, Huanghe delta saline soil ground area About 44.29 ten thousand hm2,50% or more of land area in occupied area, low-lying and coastal region is even more heavy salinizedization.Alkaline land soil It is hardened, aeration is poor, bulk density is high, soil permeability coefficient is low, capillarity is strong;Soil microbial activities are poor, and the content of organic matter is low, Nutrient dificiency;Saline Alkali Stress causes plant physiology arid, the low-quality difference of crop yield.Therefore, salt-soda soil is due to ecological environment Fragility, shortage of fresh water, vegetation are few and single, soil fertility is low, have seriously affected land comprehensive economic benefit.Currently, native Ground salination has become the serious important bottleneck problem for restricting yellow tessellated region agricultural economy and social development, seeks effectively to change Good measure has a very important significance reasonable utilize in salt-soda soil.
The improvement for salt-soda soil mainly has the ameliorative measures such as physics, chemistry and biology both at home and abroad.Physical method is improved To elute the engineering measure based on salt discharge, in conjunction with plough deeply improve the soil, soil removal and replacement, elution, the measures such as siltation reach improved effect.Chemistry side Method improvement is exactly to apply some acid salts substances, and the soil physical property in salt-soda soil is improved in conjunction with application of organic fertilizers.Though Some achievements so are achieved in terms of physics and chemical method improvement salt-soda soil both at home and abroad, but there are project amounts for physical method Greatly, at high cost, chemical method easily causes secondary pollution problems.During alkaline land improving, using measures such as physics, chemistry Very big effect is achieved, but it is big, costly to exist simultaneously such as project amount, except the salt ions such as Na, Cl row in improved, process Outside walking, mineral matter element necessary to some plants such as P, Fe, Mn and Zn etc. are also discharged simultaneously in soil, and there are undergrounds Water, downstream water source are contaminated and press salt effect to be difficult to the defects of consolidating, and researcher gradually specifies the purpose of the reclamation of salinep-alkali soil It is not only to desalt, it is often more important that reach stable high yield, that is to say, that salinity should be excluded, again fertilizing soil, then started Pay close attention to biological improvement measure.
Biological modification improves salt-soda soil using microorganism and salt-tolerant plant as key element, and, effect simple and easy with its is held It is gradually taken seriously long, it has also become the important channel of alkaline land improving, it is considered to be improve the most economical effective side in salt-soda soil One of formula.It is the key that plant improvement using salt-tolerant plant improvement saline-alkali soil.Since plant can reduce evaporation from land, infantile feverish perspiration hair Effect is transpiration, and lowering of watertable accelerates the elution of salinity, and the table for reducing salinity is poly-, reduces soil salt content.Plant The organic acid of root system secretion can improve the acid-base property of soil, reduction soil pH value, improvement soil texture etc.;Salt-tolerant plant may be used also The salinity in soil is absorbed, salinity is stored in vacuole, has not only avoided itself being poisoned, but also reduces the salt content of soil.This Outer plant carries out salt-soda soil to be also manifested by the increasing to the quantity of useful microbe population of alkaline land soil in effective improved, process Add.
It is a kind of effective measure using microorganism improvement salt-soda soil, and to the effect of alkaline land soil repairing and treating Fruit is than more significant.Applying microbial manure improvement salt-soda soil is developed on the basis of microbial manure and organic fertilizer improvement salt-soda soil Out, have a large amount of research shows that the application microbial manure or addition microorganism formulation of adaptation to local conditions can increase soil The earth content of organic matter improves soil physico-chemical property, increases the gas permeability of soil, promotes soil water-reataining fertilizer-keeping ability, to a certain degree On can influence soil water-stable aggregates movement, reach control salt effect, increase soil fertility simultaneously, promote plant growth, enhance saline-alkali tolerant Ability, to realize improvement salt-soda soil effect.Investigate, separate and identify that saline and alkaline geomicrobes are the weights of saline and alkaline geomicrobes improvement Want premise.Studies have shown that microorganism mainly has bacterium (including actinomyces), archaeal and fungi, but different salt in salinized soil The biological community structure of basicity soil differs greatly, and bacterium has different dependences to salinization and alkalization.For example, firmicutes Door (Firmicutes) is only grown in low salt affected soil, and Bacteroidetes (Bacteroidetes) only goes out in high salinity soil It is existing.The extreme saline-alkali soil in China northeast separates and identifies 20 plants of bacteriums, the classification one with the salt-soda soil Mexico Texcoco bacterium It causes.The dominant microflora of Xinjiang of China and coastal saline-alkali soil microorganism is also similar.Huanghe delta salt affected soil fungi Monoid reaches 5 59 mesh 157 and belongs to, wherein the diversity highest of bare land, Ascomycota (Ascomycota) and Basidiomycota (Basidiomycota) relative abundance is successively successively decreased.Arbuscular mycorrhizal fungi (the Arbuscular that different salt-soda soils are separated to Mycorrhizal fungi, AMF) belong to Paraglomus (Glomus) mostly.Separation obtains 47 in the Soda saline and alkaline soils of Daqing Strain moderate halophilic bacteria and 10 plants of extremely halophilic archaeas.Moderate halophilic bacteria is distributed in alkali bacillus category (Alkalibacillus), Bacillus (Bacillus) and Halomonas (Halomonas), extremely halophilic archaea belong to salt terrestrial Pseudomonas (Haloterrigena), Halophiles denitrogenation Halophilic Bacillus (Virgibacillushalodenitrificans) and cured fish Bacillus (Piscibacillussalipiscarius).As it can be seen that edaphon flora complicated composition, and different type Salt-soda soil microbial flora is different, to reach preferable improvement and improved effect, need to assemble for different salt-soda soils Different microbial flora and rational allocation its ratio.
Summary of the invention
The technical problem to be solved in the present invention is that repairing the side on Huanghe delta alkali ground using Chinese silvergrass and biomass carbon Method.The method formulates corresponding restorative procedure for the microbiologic population of Saline-alkaline Soils of Yellow River Delta and saline and alkaline parameter.
The present invention is achieved by the following technical solution:
Using southern reed and the method for charcoal in-situ immobilization Huang triangle saline field ecosystem, including 1) charcoal addition, 2) Chinese silvergrass plantation, 3) harvesting processing;
Step 1) the charcoal addition specifically: calculated according to soil acidity or alkalinity, salt content and soil property, Huang three The soil pH 8.5 of angle beach saline land, salt content 0.75%, soil property are sandy glutinous loam, and the content of organic matter is low, charcoal The 2%-4% of amount of application soil weight;Method of administration: 1, leveling land, and 2, deep according to improvement place soil density and plantation Degree calculates charcoal additive amount, and the additive amount of charcoal is the 2-4% of soil weight, and the calculation method of soil weight is soil weight Amount=improvement place surface area * planting depth * soil density;3, the charcoal calculated is uniformly layered on soil surface, turned over Soil is mixed thoroughly;4, it mixes post curing thoroughly three days, southern reed Chinese silvergrass can be planted;The charcoal is furfural dregs, and phosphorus content is greater than 70%, containing the mineral matter element including iron, manganese, copper, 60-150 square metres every gram of specific surface area.
Step 2) the Chinese silvergrass plantation includes seedling culture, transplanting and final-period management:
The step of seedling culture is that (1) materials choose Nan Di group, selects the healthy plant of diameter 1.0-2.5cm, Aerial part is gathered in;
(2) stem section select by the aerial stem of plant with clear water clean after, more than two section of selected distance root, below two section of top Middle stem section, the intermediate position of each section is cut into shape of a hoof port, keeps every section axillary bud centrally located;Every section length 5-15cm;
(3) clear water impregnates: the stem section sheared being immersed in clear water, 4-5h is impregnated, makes the sufficiently water suction of its tissue;(4) swash Element processing: the stem section of previous step is taken out, and is placed in methyl α-naphthyl acetate (NAA) and heteroauxin (IBA) that concentration is respectively 20mg/L Mixed solution in (matching while using), impregnate 2-2.5h after take out stand;
(5) culture substrate is prepared: humus, laterite, vermiculite, sandy soil are with volume ratio 2:2:1:1 mixing, watering to water content 30%;
(6) pallet is sowed: configured culture substrate being laid in seedlings nursing plate, culture substrate will walk with a thickness of 5-7cm Suddenly the stem section horizontal homogeneous of (4) is layered on culture substrate, and the distance between stem section is 3-5cm;The good rear surface of tiling covers training Matrix is supported, thickness 5cm sprays water mist, comes into full contact with stem section with culture substrate, not fall through, and environment temperature is 22-26 DEG C, Intensity of illumination is 1500-3000Lx;
(7) training orientation: watering is primary daily, using sprinkling or spray pattern, so that matrix is kept wet, 10 days deutomerite Segment length goes out adventitious root, and sprout starts to sprout, and seedling reaches 10-15cm in 15 days plant heights;Seedling replanting: Seedling Height can after 23 days Up to 25-35cm, plant is carefully dug out from matrix, is transplanted;
The transplanting and final-period management: digging diameter 15cm, the hole of depth 10-15cm, hole spacing 50-80cm, often A hole puts the 2-3 plants of seedling cultivated, and a water is poured after earthing, until maxmun field capacity 70-80%, is poured again after 3-5 days Water is kept to maxmun field capacity 50-60%;Later period regards weather condition watering.
3) the Chinese silvergrass harvesting processing: Chinese silvergrass harvesting, when 1.5 meters of Chinese silvergrass plant height or so, unified harvesting is apart from ground 20- The ground cauline leaf of 50cm or more, is made into ensilage.
Further, it is sprayed at using lactobacillus plantarum EC018 with cheese type lactobacillus with thalline quantity ratio 1:1 mixed bacteria liquid Chinese silvergrass surface, is made into ensilage;The preparation method of the lactobacillus plantarum EC018: using heavy ion radiation method of mutagenesis, right Common plant lactobacillus carries out heavy ion radiation mutagenesis, and mutagenesis energy heavy ion beam current is12C6+, energy 80MeV/u, radiation Dosage is 50GY, radiated time 5min, and the lactobacillus plantarum after irradiation obtains after the screening of plate streaking culture repeatedly, in 2018 It is deposited in China General Microbiological culture presevation administrative center on October 8, deposit number is CGMCC NO.:16556, preservation Location Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3;
The present invention compared with prior art the utility model has the advantages that
Technical solution of the present invention can carry out in-situ immobilization to the saline field ecosystem in Yellow River Delta Coastal area, repair Great changes will take place for alkaline land soil physicochemical characteristics and biology community structure after multiple, can be suitable for that Chinese silvergrass survives and normally gives birth to It is long.The Chinese silvergrass planted in improvement is made into ensilage using microbial technique, and the Chinese silvergrass ensiling after undergoing microbial fermentation is raised Material has good palatability, and is able to satisfy the nutritional need of animal.
Since the biocoene in each salt-soda soil and saline and alkaline have differences, the method for the present invention is only to be suitable for Huang three The improvement of angle beach saline land.The method of the present invention by using furfural dregs as charcoal, in combination with southern reed Chinese silvergrass to Huang three Angle coastal saline-alkali improve, significant effect.
Chinese silvergrass is higher due to its content of cellulose under normal circumstances, is not suitable for directly as animal forage grass, and of the invention Using the bacterial strain newly screened, Chinese silvergrass is made into ensilage by microbial fermentation processes, while salt-soda soil obtains improvement, New raw material is provided for deficient animal silage source.
Detailed description of the invention
Fig. 1, (CK is control, BC1 and BC2 to the salt affected soil physical characteristic change after salt tolerant Chinese silvergrass and charcoal improvement Respectively different charcoal additive amount);
Fig. 2, the salt affected soil biological community structure variation after salt tolerant Chinese silvergrass and charcoal improvement.
Specific embodiment
The present invention will be further described with example with reference to the accompanying drawing, but protection scope of the present invention is not by embodiment Limitation in any form.
Embodiment 1: Chinese silvergrass kind used is southern reed, and charcoal is furfural dregs.
Using southern reed and the method for charcoal in-situ immobilization Huang triangle saline field ecosystem, including 1) modifying agent addition, 2) Chinese silvergrass plantation, 3) harvesting processing;
Step 1) the modifying agent addition specifically: soil ph 8.5, salt content 0.75%, soil property are sandy glutinous earth Soil, the content of organic matter is low, then is suitable for 3% charcoal of application.Method of administration: 1, level land, 2, according to improvement place soil it is close Degree 2.01 × 103㎏/m3, planting depth 20cm calculates the additive amount of charcoal is how many? the additive amount of charcoal is soil The 3% of weight, the calculation method of soil weight are soil weight=improvement place surface area * planting depth * soil density, example If the additive amount of every square metre of charcoal is 12kg;3, the charcoal calculated is uniformly layered on soil surface, dig 20-30 Centimetre, is mixed thoroughly;4, it mixes post curing thoroughly three days, plant or crop can be sowed;
Step 2) the Chinese silvergrass plantation includes seedling culture, transplanting and final-period management:
The step of seedling culture is that (1) materials choose Nan Di group, selects the healthy plant of diameter 1.0-2.5cm, Aerial part is gathered in;
(2) stem section select by the aerial stem of plant with clear water clean after, more than two section of selected distance root, below two section of top Middle stem section, the intermediate position of each section is cut into shape of a hoof port, keeps every section axillary bud centrally located;Every section length 5-15cm;
(3) clear water impregnates: the stem section sheared being immersed in clear water, 4-5h is impregnated, makes the sufficiently water suction of its tissue;(4) swash Element processing: the stem section of previous step is taken out, and is placed in methyl α-naphthyl acetate (NAA) and heteroauxin (IBA) that concentration is respectively 20mg/L Mixed solution in (matching while using), impregnate 2-2.5h after take out stand;
(5) culture substrate is prepared: humus, laterite, vermiculite, sandy soil are with volume ratio 2:2:1:1 mixing, watering to water content 30%;
(6) pallet is sowed: configured culture substrate being laid in seedlings nursing plate, culture substrate will walk with a thickness of 5-7cm Suddenly the stem section horizontal homogeneous of (4) is layered on culture substrate, and the distance between stem section is 3-5cm;The good rear surface of tiling covers training Matrix is supported, thickness 5cm sprays water mist, comes into full contact with stem section with culture substrate, not fall through, and environment temperature is 22-26 DEG C, Intensity of illumination is 1500-3000Lx;
(7) training orientation: watering is primary daily, using sprinkling or spray pattern, so that matrix is kept wet, 10 days deutomerite Segment length goes out adventitious root, and sprout starts to sprout, and seedling reaches 10-15cm in 15 days plant heights;Seedling replanting: Seedling Height can after 23 days Up to 25-35cm, plant is carefully dug out from matrix, is transplanted;
The transplanting and final-period management: digging diameter 15cm, the hole of depth 10-15cm, hole spacing 50-80cm, often A hole puts the 2-3 plants of seedling cultivated, and a water is poured after earthing, until maxmun field capacity 70% or so, is poured again after 3-5 days Water is kept to maxmun field capacity 50% or so;Later period regards weather condition watering.
3) the Chinese silvergrass harvesting processing: Chinese silvergrass harvesting, when 1.5 meters of Chinese silvergrass plant height or so, unified harvesting is apart from ground 20- The ground cauline leaf of 50cm or more, is made into ensilage.Specially the Chinese silvergrass of harvesting is dried one day, water content to 20% or so, Chinese silvergrass ground cauline leaf is cut into the segment 4-8cm, is mixed;Harvested clover is dried one day.By after sunning Chinese silvergrass and lucerne Mu is stirred mixing according to mass ratio 7:3, sprays lactobacillus mixed bacteria liquid, and the lactobacillus mixed bacteria liquid is plant cream bar Bacterium EC018 is mixed with cheese type lactobacillus with thalline quantity ratio 1:1;It is big to carry out ensiling (joint ensilage machine can be used) storage After 30 days.The preparation method of the lactobacillus plantarum EC018: heavy ion radiation method of mutagenesis is used, to common plant Lactobacillus carries out heavy ion radiation mutagenesis, and mutagenesis energy heavy ion beam current is12C6+, energy 80MeV/u, dose of radiation is 50GY, radiated time 5min, the lactobacillus plantarum after irradiation obtains after the screening of plate streaking culture repeatedly, in October, 2018 It is deposited within 8th China General Microbiological culture presevation administrative center, deposit number is CGMCC NO.:16556, preservation address Beijing The institute 3 of city Chaoyang District North Star West Road 1.Chinese silvergrass feed nutrient measurement after ensiling is as shown in table 1, by the side Method corrodes fermented material from other harmful miscellaneous bacterias the pH value that can not only reduce ensiling material after Chinese silvergrass progress ensiling, Effective protection ensilage;And containing for soluble-carbohydrate and protein in Chinese silvergrass feed can also be improved after fermentation Amount reduces ammonia nitrogen content, effectively prevents the degradation of protein in feed, protect the loss of nutriment, reduce feeding The content of neutral detergent fiber and acid detergent fiber in material, improves the nutritional quality of feed, increases the feeding valence of feed Value;The content for increasing organic acid content in feed, especially lactic acid simultaneously, improves the palatability of feed, improves the mouth of feed Sense and nutrient absorption value.
Chinese silvergrass Silage Quality under 1 microbial action of table
By measurement addition charcoal and salt tolerant Chinese silvergrass plantation front and back salt affected soil items physical and chemical index, soil after discovery improvement Earth salt content significantly reduces, and the soil organism and fertility step up (Fig. 1).It is found by high-flux sequence, added biology The structure of soil microbial community in the salt-soda soil after charcoal and plantation salt tolerant Chinese silvergrass improvement significantly changes (figure before relatively improveing 2), these results indicate that charcoal improves and salt tolerant Chinese silvergrass is combined to plant the soil physical and chemical property that can significantly improve salt-soda soil, this Mainly realized by the structure of community and activity that improve edaphon.
While above-described embodiment is carried out, using traditional ardealite and desulfurized gypsum to the scattered angle beach saline land of Huang into Control improved test is gone, the usage amount of ardealite and desulfurized gypsum is 22500-37500kg/hm2;After the test, to three Kind improved results are compared, as shown in table 2.
The comparison of test results that 2. 3 kinds of methods of table improve yellow triangle beach saline land

Claims (5)

1.利用南荻及生物炭原位修复黄三角盐碱地生态系统的方法,其特征在于所述方法包括1)生物炭添加、2)芒草种植、3)收割处理;1. the method for in-situ restoration of the Yellow Triangle saline-alkali land ecosystem utilizing Nadi and biochar, is characterized in that the method comprises 1) biochar addition, 2) Miscanthus planting, 3) harvesting treatment; 所述步骤1)生物炭添加具体为:根据土壤酸碱度、含盐量以及土壤性质计算,黄三角滨海盐碱地的土壤pH8.5,含盐量0.75%,土壤性质为沙质黏壤土,有机质含量低,生物炭施用量土壤重量的2%-4%;施用方法:(1)平整土地,(2)按照改良地点土壤密度以及种植深度计算生物炭具体添加量,生物炭的具体添加量为土壤重量的2-4%,土壤重量的计算方法为土壤重量=改良地点的表面积*种植深度*土壤密度;(3)将计算好的生物炭均匀铺在土壤表面,翻土,拌匀;(4)拌匀后熟化三天,即可种植南荻芒草;所述的生物炭为糠醛渣,其含碳量大于70%,含有包括铁、锰、铜在内的矿物质元素,比表面积60-150平方米每克。The step 1) biochar addition is as follows: according to soil pH, salt content and soil properties, the soil pH of the Yellow Delta coastal saline-alkali land is 8.5, the salt content is 0.75%, the soil properties are sandy clay loam, and the organic matter content is low , the application amount of biochar is 2%-4% of the soil weight; application method: (1) leveling the land, (2) calculating the specific amount of biochar added according to the soil density and planting depth of the improvement site, the specific amount of biochar added is the soil weight The calculation method of soil weight is soil weight = surface area of improvement site * planting depth * soil density; (3) Evenly spread the calculated biochar on the soil surface, turn the soil, and mix well; (4) After being mixed and matured for three days, Miscanthus can be planted; the biochar is furfural residue, its carbon content is more than 70%, and it contains mineral elements including iron, manganese and copper, with a specific surface area of 60-150 square meters meters per gram. 2.根据权利要求1所述的利用南荻及生物炭原位修复黄三角盐碱地生态系统的方法,其特征在于所述步骤2)芒草种植包括幼苗培育、移栽及后期管理:2. the method for in-situ restoration of the Yellow Triangle saline-alkali land ecosystem utilizing Nadi and biochar according to claim 1, is characterized in that described step 2) Miscanthus planting comprises seedling cultivation, transplanting and later stage management: 所述幼苗培育为(1)取材选取南荻群落,挑选直径1.0-2.5cm的健康植株,将地上部分收割;Described seedling cultivation is (1) drawing material and selecting Nandi community, selecting healthy plants with a diameter of 1.0-2.5cm, and harvesting the aerial part; (2)茎段挑选将地上茎用清水洗净后,选取距离根部两节以上、距离顶部两节以下的中间茎段,把每一节的中间部位裁剪成马蹄形端口,使每节腋芽位于中间位置;每节长度5-15cm;(2) Selection of stem segments After the above-ground stems are washed with water, the middle stem segments that are more than two nodes away from the root and less than two nodes away from the top are selected, and the middle part of each node is cut into a horseshoe-shaped port, so that the axillary buds of each node are located in the middle position ;The length of each section is 5-15cm; (3)清水浸泡:将剪好的茎段浸没于清水中,浸泡4-5h,使其组织充分吸水;(3) Soak in water: immerse the cut stem segments in clean water for 4-5h to make the tissue fully absorb water; (4)激素处理:将上一步骤的茎段取出,置于浓度分别为20mg/L的萘乙酸与吲哚乙酸的混合溶液中,浸泡2-2.5h后取出静置;(4) Hormone treatment: take out the stem segment of the previous step, place it in a mixed solution of naphthalene acetic acid and indole acetic acid with a concentration of 20 mg/L, soak it for 2-2.5 h, take it out and let it stand; (5)培养基质配制:腐殖质、红土、蛭石、沙土以体积比2:2:1:1混合,浇水至含水重量比30%;(5) Preparation of culture medium: humus, laterite, vermiculite, and sand are mixed in a volume ratio of 2:2:1:1, and watered to a water weight ratio of 30%; (6)托盘播种:将配置好的培养基质平铺于育苗盘中,培养基质厚度为5-7cm,将步骤(4)的茎段铺在培养基质上,茎段之间的距离为3-5cm;平铺好之后表面覆盖培养基质,厚度5cm,喷施水雾,环境温度为22-26℃,光照强度为1500-3000Lx;(6) tray sowing: the configured culture medium is spread in the seedling tray, the thickness of the culture medium is 5-7cm, the stem section of step (4) is spread on the culture medium, and the distance between the stem sections is 3-7 cm 5cm; after tiling, the surface is covered with culture medium, with a thickness of 5cm, sprayed with water mist, the ambient temperature is 22-26°C, and the light intensity is 1500-3000Lx; (7)培养管理:使基质保持湿润,幼苗在15天株高达到10-15cm;幼苗移栽:23天后幼苗株高达25-35cm时,将植株从基质中挖出,进行移栽。(7) Cultivation management: keep the substrate moist, and the seedlings reach 10-15cm in height in 15 days; Seedling transplantation: when the seedlings are up to 25-35cm in 23 days, the plants are dug out of the substrate and transplanted. 3.根据权利要求3所述的方法,其特征在于所述的移栽及后期管理:挖直径15cm,深度10-15cm的坑穴,坑穴间距50-80cm,每个坑穴放2-3株培育好的幼苗,覆土后浇一次水,至田间最大持水重量比70-80%,3-5天后再浇一次水,保持至田间最大持水重量量50-60%;后期视天气情况浇水。3. method according to claim 3, is characterized in that described transplanting and later stage management: dig diameter 15cm, the pit of depth 10-15cm, pit spacing 50-80cm, each pit is put 2-3 The cultivated seedlings should be watered once after covering the soil until the maximum water-holding weight ratio of the field is 70-80%, and then watered again after 3-5 days to maintain the maximum water-holding weight of the field at 50-60%; the later period depends on the weather conditions watering. 4.根据权利要求1所述的方法,其特征在于所述芒草收割处理:待芒草株高1.4-1.8米时,收割距离地面20-50cm以上的地上茎叶,做成青贮饲料。4. method according to claim 1, it is characterized in that described Miscanthus harvest processing: when Miscanthus plant height is 1.4-1.8 meters, harvest above ground stems and leaves more than 20-50cm from the ground, make silage. 5.根据权利要求4所述的方法,其特征在于利用芒草做成青贮饲料使用的菌株为植物乳杆菌EC018与干酪型乳杆菌,两种菌体数量比1:1混合后喷洒在芒草表面;所述植物乳杆菌EC018的获得方法:采用重离子辐射诱变方法,对普通植物乳杆菌进行重离子辐射诱变,诱变能量重离子束流为12C6+,能量为80MeV/u,辐射剂量为50GY,辐射时间5min,辐照后的植物乳杆菌经反复平板划线培养筛选后得到,于2018年10月8日保藏在中国普通微生物菌种保藏管理中心,保藏号为CGMCC NO.:16556,保藏地址北京市朝阳区北辰西路1号院3号。5. method according to claim 4, it is characterized in that utilizing Miscanthus to make the bacterial strain that silage is used is Lactobacillus plantarum EC018 and Lactobacillus casei, two kinds of thalline numbers are sprayed on Miscanthus surface after mixing 1:1; The acquisition method of described Lactobacillus plantarum EC018: adopt heavy ion radiation mutagenesis method, carry out heavy ion radiation mutagenesis to common Lactobacillus plantarum, mutagenesis energy heavy ion beam current is 12 C 6+ , energy is 80MeV/u, radiation The dose is 50GY, and the irradiation time is 5min. The irradiated Lactobacillus plantarum is obtained after repeated plate streaking and screening. It was deposited in the China General Microorganism Culture Collection and Management Center on October 8, 2018, and the preservation number is CGMCC NO.: 16556, the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110301304A (en) * 2019-07-26 2019-10-08 湖南农业大学 A kind of method of salt-soda soil plantation awns genus plant
CN110305791A (en) * 2019-06-04 2019-10-08 郑州永丰生物肥业有限公司 Method of breeding strain, microbial manure, the implantation methods of drought-enduring plant, restoration of the ecosystem method
CN110366893A (en) * 2019-07-24 2019-10-25 中国科学院东北地理与农业生态研究所 A method of addition charcoal improves saline-alkali grassland soil quality
CN114249432A (en) * 2020-09-24 2022-03-29 中蓝连海设计研究院有限公司 Biological promoter and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102250807A (en) * 2011-07-06 2011-11-23 广西工学院 Microbial agent for Chinese silvergrass ensilage as well as preparation method and application thereof
CN104303805A (en) * 2014-11-06 2015-01-28 湖南农业大学 Triarrhena lutarioriparia clonal seedling method
CN107347438A (en) * 2017-09-19 2017-11-17 中国科学院青岛生物能源与过程研究所 A kind of southern reed ground stalk quick propagation method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102250807A (en) * 2011-07-06 2011-11-23 广西工学院 Microbial agent for Chinese silvergrass ensilage as well as preparation method and application thereof
CN104303805A (en) * 2014-11-06 2015-01-28 湖南农业大学 Triarrhena lutarioriparia clonal seedling method
CN107347438A (en) * 2017-09-19 2017-11-17 中国科学院青岛生物能源与过程研究所 A kind of southern reed ground stalk quick propagation method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
夏阳: "生物炭对滨海盐碱植物生长及根际土壤环境的影响", 《中国优秀硕士学位论文全文数据库农业科技辑》 *
孙军娜等: "糠醛渣及其生物炭对盐渍土理化性质影响的比较研究", 《农业环境科学学报》 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110305791A (en) * 2019-06-04 2019-10-08 郑州永丰生物肥业有限公司 Method of breeding strain, microbial manure, the implantation methods of drought-enduring plant, restoration of the ecosystem method
CN110366893A (en) * 2019-07-24 2019-10-25 中国科学院东北地理与农业生态研究所 A method of addition charcoal improves saline-alkali grassland soil quality
CN110301304A (en) * 2019-07-26 2019-10-08 湖南农业大学 A kind of method of salt-soda soil plantation awns genus plant
CN110301304B (en) * 2019-07-26 2021-07-27 湖南农业大学 A kind of method for planting Miscanthus plants in saline-alkali land
CN114249432A (en) * 2020-09-24 2022-03-29 中蓝连海设计研究院有限公司 Biological promoter and application thereof
CN114249432B (en) * 2020-09-24 2023-03-21 中蓝连海设计研究院有限公司 Biological promoter and application thereof

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Application publication date: 20190205