Utilize southern reed and the method for charcoal in-situ immobilization Huang triangle saline field ecosystem
Technical field
The invention belongs to ecology of alkali saline land to repair field, more particularly to a kind of yellow using southern reed and charcoal in-situ immobilization
The method of triangle saline field ecosystem.
Background technique
The salinization of soil is a global problem.China is third place in the world big salt-soda soil distribution country.The Huanghe delta
Be land-sea interaction formed move back Haiti, annual rainfall is few, and evaporation capacity is big, and soil salinization phenomenon is very general in the region
Time.According to soil salt content, salt-soda soil can be divided into slight, moderate, heavy salinized, and general slight alkaline land soil salt content is
0.1%~0.3%, moderate is 0.3%~0.6%, and severe is > 0.6%.According to statistics, Huanghe delta saline soil ground area
About 44.29 ten thousand hm2,50% or more of land area in occupied area, low-lying and coastal region is even more heavy salinizedization.Alkaline land soil
It is hardened, aeration is poor, bulk density is high, soil permeability coefficient is low, capillarity is strong;Soil microbial activities are poor, and the content of organic matter is low,
Nutrient dificiency;Saline Alkali Stress causes plant physiology arid, the low-quality difference of crop yield.Therefore, salt-soda soil is due to ecological environment
Fragility, shortage of fresh water, vegetation are few and single, soil fertility is low, have seriously affected land comprehensive economic benefit.Currently, native
Ground salination has become the serious important bottleneck problem for restricting yellow tessellated region agricultural economy and social development, seeks effectively to change
Good measure has a very important significance reasonable utilize in salt-soda soil.
The improvement for salt-soda soil mainly has the ameliorative measures such as physics, chemistry and biology both at home and abroad.Physical method is improved
To elute the engineering measure based on salt discharge, in conjunction with plough deeply improve the soil, soil removal and replacement, elution, the measures such as siltation reach improved effect.Chemistry side
Method improvement is exactly to apply some acid salts substances, and the soil physical property in salt-soda soil is improved in conjunction with application of organic fertilizers.Though
Some achievements so are achieved in terms of physics and chemical method improvement salt-soda soil both at home and abroad, but there are project amounts for physical method
Greatly, at high cost, chemical method easily causes secondary pollution problems.During alkaline land improving, using measures such as physics, chemistry
Very big effect is achieved, but it is big, costly to exist simultaneously such as project amount, except the salt ions such as Na, Cl row in improved, process
Outside walking, mineral matter element necessary to some plants such as P, Fe, Mn and Zn etc. are also discharged simultaneously in soil, and there are undergrounds
Water, downstream water source are contaminated and press salt effect to be difficult to the defects of consolidating, and researcher gradually specifies the purpose of the reclamation of salinep-alkali soil
It is not only to desalt, it is often more important that reach stable high yield, that is to say, that salinity should be excluded, again fertilizing soil, then started
Pay close attention to biological improvement measure.
Biological modification improves salt-soda soil using microorganism and salt-tolerant plant as key element, and, effect simple and easy with its is held
It is gradually taken seriously long, it has also become the important channel of alkaline land improving, it is considered to be improve the most economical effective side in salt-soda soil
One of formula.It is the key that plant improvement using salt-tolerant plant improvement saline-alkali soil.Since plant can reduce evaporation from land, infantile feverish perspiration hair
Effect is transpiration, and lowering of watertable accelerates the elution of salinity, and the table for reducing salinity is poly-, reduces soil salt content.Plant
The organic acid of root system secretion can improve the acid-base property of soil, reduction soil pH value, improvement soil texture etc.;Salt-tolerant plant may be used also
The salinity in soil is absorbed, salinity is stored in vacuole, has not only avoided itself being poisoned, but also reduces the salt content of soil.This
Outer plant carries out salt-soda soil to be also manifested by the increasing to the quantity of useful microbe population of alkaline land soil in effective improved, process
Add.
It is a kind of effective measure using microorganism improvement salt-soda soil, and to the effect of alkaline land soil repairing and treating
Fruit is than more significant.Applying microbial manure improvement salt-soda soil is developed on the basis of microbial manure and organic fertilizer improvement salt-soda soil
Out, have a large amount of research shows that the application microbial manure or addition microorganism formulation of adaptation to local conditions can increase soil
The earth content of organic matter improves soil physico-chemical property, increases the gas permeability of soil, promotes soil water-reataining fertilizer-keeping ability, to a certain degree
On can influence soil water-stable aggregates movement, reach control salt effect, increase soil fertility simultaneously, promote plant growth, enhance saline-alkali tolerant
Ability, to realize improvement salt-soda soil effect.Investigate, separate and identify that saline and alkaline geomicrobes are the weights of saline and alkaline geomicrobes improvement
Want premise.Studies have shown that microorganism mainly has bacterium (including actinomyces), archaeal and fungi, but different salt in salinized soil
The biological community structure of basicity soil differs greatly, and bacterium has different dependences to salinization and alkalization.For example, firmicutes
Door (Firmicutes) is only grown in low salt affected soil, and Bacteroidetes (Bacteroidetes) only goes out in high salinity soil
It is existing.The extreme saline-alkali soil in China northeast separates and identifies 20 plants of bacteriums, the classification one with the salt-soda soil Mexico Texcoco bacterium
It causes.The dominant microflora of Xinjiang of China and coastal saline-alkali soil microorganism is also similar.Huanghe delta salt affected soil fungi
Monoid reaches 5 59 mesh 157 and belongs to, wherein the diversity highest of bare land, Ascomycota (Ascomycota) and Basidiomycota
(Basidiomycota) relative abundance is successively successively decreased.Arbuscular mycorrhizal fungi (the Arbuscular that different salt-soda soils are separated to
Mycorrhizal fungi, AMF) belong to Paraglomus (Glomus) mostly.Separation obtains 47 in the Soda saline and alkaline soils of Daqing
Strain moderate halophilic bacteria and 10 plants of extremely halophilic archaeas.Moderate halophilic bacteria is distributed in alkali bacillus category (Alkalibacillus),
Bacillus (Bacillus) and Halomonas (Halomonas), extremely halophilic archaea belong to salt terrestrial Pseudomonas
(Haloterrigena), Halophiles denitrogenation Halophilic Bacillus (Virgibacillushalodenitrificans) and cured fish
Bacillus (Piscibacillussalipiscarius).As it can be seen that edaphon flora complicated composition, and different type
Salt-soda soil microbial flora is different, to reach preferable improvement and improved effect, need to assemble for different salt-soda soils
Different microbial flora and rational allocation its ratio.
Summary of the invention
The technical problem to be solved in the present invention is that repairing the side on Huanghe delta alkali ground using Chinese silvergrass and biomass carbon
Method.The method formulates corresponding restorative procedure for the microbiologic population of Saline-alkaline Soils of Yellow River Delta and saline and alkaline parameter.
The present invention is achieved by the following technical solution:
Using southern reed and the method for charcoal in-situ immobilization Huang triangle saline field ecosystem, including 1) charcoal addition,
2) Chinese silvergrass plantation, 3) harvesting processing;
Step 1) the charcoal addition specifically: calculated according to soil acidity or alkalinity, salt content and soil property, Huang three
The soil pH 8.5 of angle beach saline land, salt content 0.75%, soil property are sandy glutinous loam, and the content of organic matter is low, charcoal
The 2%-4% of amount of application soil weight;Method of administration: 1, leveling land, and 2, deep according to improvement place soil density and plantation
Degree calculates charcoal additive amount, and the additive amount of charcoal is the 2-4% of soil weight, and the calculation method of soil weight is soil weight
Amount=improvement place surface area * planting depth * soil density;3, the charcoal calculated is uniformly layered on soil surface, turned over
Soil is mixed thoroughly;4, it mixes post curing thoroughly three days, southern reed Chinese silvergrass can be planted;The charcoal is furfural dregs, and phosphorus content is greater than
70%, containing the mineral matter element including iron, manganese, copper, 60-150 square metres every gram of specific surface area.
Step 2) the Chinese silvergrass plantation includes seedling culture, transplanting and final-period management:
The step of seedling culture is that (1) materials choose Nan Di group, selects the healthy plant of diameter 1.0-2.5cm,
Aerial part is gathered in;
(2) stem section select by the aerial stem of plant with clear water clean after, more than two section of selected distance root, below two section of top
Middle stem section, the intermediate position of each section is cut into shape of a hoof port, keeps every section axillary bud centrally located;Every section length
5-15cm;
(3) clear water impregnates: the stem section sheared being immersed in clear water, 4-5h is impregnated, makes the sufficiently water suction of its tissue;(4) swash
Element processing: the stem section of previous step is taken out, and is placed in methyl α-naphthyl acetate (NAA) and heteroauxin (IBA) that concentration is respectively 20mg/L
Mixed solution in (matching while using), impregnate 2-2.5h after take out stand;
(5) culture substrate is prepared: humus, laterite, vermiculite, sandy soil are with volume ratio 2:2:1:1 mixing, watering to water content
30%;
(6) pallet is sowed: configured culture substrate being laid in seedlings nursing plate, culture substrate will walk with a thickness of 5-7cm
Suddenly the stem section horizontal homogeneous of (4) is layered on culture substrate, and the distance between stem section is 3-5cm;The good rear surface of tiling covers training
Matrix is supported, thickness 5cm sprays water mist, comes into full contact with stem section with culture substrate, not fall through, and environment temperature is 22-26 DEG C,
Intensity of illumination is 1500-3000Lx;
(7) training orientation: watering is primary daily, using sprinkling or spray pattern, so that matrix is kept wet, 10 days deutomerite
Segment length goes out adventitious root, and sprout starts to sprout, and seedling reaches 10-15cm in 15 days plant heights;Seedling replanting: Seedling Height can after 23 days
Up to 25-35cm, plant is carefully dug out from matrix, is transplanted;
The transplanting and final-period management: digging diameter 15cm, the hole of depth 10-15cm, hole spacing 50-80cm, often
A hole puts the 2-3 plants of seedling cultivated, and a water is poured after earthing, until maxmun field capacity 70-80%, is poured again after 3-5 days
Water is kept to maxmun field capacity 50-60%;Later period regards weather condition watering.
3) the Chinese silvergrass harvesting processing: Chinese silvergrass harvesting, when 1.5 meters of Chinese silvergrass plant height or so, unified harvesting is apart from ground 20-
The ground cauline leaf of 50cm or more, is made into ensilage.
Further, it is sprayed at using lactobacillus plantarum EC018 with cheese type lactobacillus with thalline quantity ratio 1:1 mixed bacteria liquid
Chinese silvergrass surface, is made into ensilage;The preparation method of the lactobacillus plantarum EC018: using heavy ion radiation method of mutagenesis, right
Common plant lactobacillus carries out heavy ion radiation mutagenesis, and mutagenesis energy heavy ion beam current is12C6+, energy 80MeV/u, radiation
Dosage is 50GY, radiated time 5min, and the lactobacillus plantarum after irradiation obtains after the screening of plate streaking culture repeatedly, in 2018
It is deposited in China General Microbiological culture presevation administrative center on October 8, deposit number is CGMCC NO.:16556, preservation
Location Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3;
The present invention compared with prior art the utility model has the advantages that
Technical solution of the present invention can carry out in-situ immobilization to the saline field ecosystem in Yellow River Delta Coastal area, repair
Great changes will take place for alkaline land soil physicochemical characteristics and biology community structure after multiple, can be suitable for that Chinese silvergrass survives and normally gives birth to
It is long.The Chinese silvergrass planted in improvement is made into ensilage using microbial technique, and the Chinese silvergrass ensiling after undergoing microbial fermentation is raised
Material has good palatability, and is able to satisfy the nutritional need of animal.
Since the biocoene in each salt-soda soil and saline and alkaline have differences, the method for the present invention is only to be suitable for Huang three
The improvement of angle beach saline land.The method of the present invention by using furfural dregs as charcoal, in combination with southern reed Chinese silvergrass to Huang three
Angle coastal saline-alkali improve, significant effect.
Chinese silvergrass is higher due to its content of cellulose under normal circumstances, is not suitable for directly as animal forage grass, and of the invention
Using the bacterial strain newly screened, Chinese silvergrass is made into ensilage by microbial fermentation processes, while salt-soda soil obtains improvement,
New raw material is provided for deficient animal silage source.
Detailed description of the invention
Fig. 1, (CK is control, BC1 and BC2 to the salt affected soil physical characteristic change after salt tolerant Chinese silvergrass and charcoal improvement
Respectively different charcoal additive amount);
Fig. 2, the salt affected soil biological community structure variation after salt tolerant Chinese silvergrass and charcoal improvement.
Specific embodiment
The present invention will be further described with example with reference to the accompanying drawing, but protection scope of the present invention is not by embodiment
Limitation in any form.
Embodiment 1: Chinese silvergrass kind used is southern reed, and charcoal is furfural dregs.
Using southern reed and the method for charcoal in-situ immobilization Huang triangle saline field ecosystem, including 1) modifying agent addition,
2) Chinese silvergrass plantation, 3) harvesting processing;
Step 1) the modifying agent addition specifically: soil ph 8.5, salt content 0.75%, soil property are sandy glutinous earth
Soil, the content of organic matter is low, then is suitable for 3% charcoal of application.Method of administration: 1, level land, 2, according to improvement place soil it is close
Degree 2.01 × 103㎏/m3, planting depth 20cm calculates the additive amount of charcoal is how many? the additive amount of charcoal is soil
The 3% of weight, the calculation method of soil weight are soil weight=improvement place surface area * planting depth * soil density, example
If the additive amount of every square metre of charcoal is 12kg;3, the charcoal calculated is uniformly layered on soil surface, dig 20-30
Centimetre, is mixed thoroughly;4, it mixes post curing thoroughly three days, plant or crop can be sowed;
Step 2) the Chinese silvergrass plantation includes seedling culture, transplanting and final-period management:
The step of seedling culture is that (1) materials choose Nan Di group, selects the healthy plant of diameter 1.0-2.5cm,
Aerial part is gathered in;
(2) stem section select by the aerial stem of plant with clear water clean after, more than two section of selected distance root, below two section of top
Middle stem section, the intermediate position of each section is cut into shape of a hoof port, keeps every section axillary bud centrally located;Every section length
5-15cm;
(3) clear water impregnates: the stem section sheared being immersed in clear water, 4-5h is impregnated, makes the sufficiently water suction of its tissue;(4) swash
Element processing: the stem section of previous step is taken out, and is placed in methyl α-naphthyl acetate (NAA) and heteroauxin (IBA) that concentration is respectively 20mg/L
Mixed solution in (matching while using), impregnate 2-2.5h after take out stand;
(5) culture substrate is prepared: humus, laterite, vermiculite, sandy soil are with volume ratio 2:2:1:1 mixing, watering to water content
30%;
(6) pallet is sowed: configured culture substrate being laid in seedlings nursing plate, culture substrate will walk with a thickness of 5-7cm
Suddenly the stem section horizontal homogeneous of (4) is layered on culture substrate, and the distance between stem section is 3-5cm;The good rear surface of tiling covers training
Matrix is supported, thickness 5cm sprays water mist, comes into full contact with stem section with culture substrate, not fall through, and environment temperature is 22-26 DEG C,
Intensity of illumination is 1500-3000Lx;
(7) training orientation: watering is primary daily, using sprinkling or spray pattern, so that matrix is kept wet, 10 days deutomerite
Segment length goes out adventitious root, and sprout starts to sprout, and seedling reaches 10-15cm in 15 days plant heights;Seedling replanting: Seedling Height can after 23 days
Up to 25-35cm, plant is carefully dug out from matrix, is transplanted;
The transplanting and final-period management: digging diameter 15cm, the hole of depth 10-15cm, hole spacing 50-80cm, often
A hole puts the 2-3 plants of seedling cultivated, and a water is poured after earthing, until maxmun field capacity 70% or so, is poured again after 3-5 days
Water is kept to maxmun field capacity 50% or so;Later period regards weather condition watering.
3) the Chinese silvergrass harvesting processing: Chinese silvergrass harvesting, when 1.5 meters of Chinese silvergrass plant height or so, unified harvesting is apart from ground 20-
The ground cauline leaf of 50cm or more, is made into ensilage.Specially the Chinese silvergrass of harvesting is dried one day, water content to 20% or so,
Chinese silvergrass ground cauline leaf is cut into the segment 4-8cm, is mixed;Harvested clover is dried one day.By after sunning Chinese silvergrass and lucerne
Mu is stirred mixing according to mass ratio 7:3, sprays lactobacillus mixed bacteria liquid, and the lactobacillus mixed bacteria liquid is plant cream bar
Bacterium EC018 is mixed with cheese type lactobacillus with thalline quantity ratio 1:1;It is big to carry out ensiling (joint ensilage machine can be used) storage
After 30 days.The preparation method of the lactobacillus plantarum EC018: heavy ion radiation method of mutagenesis is used, to common plant
Lactobacillus carries out heavy ion radiation mutagenesis, and mutagenesis energy heavy ion beam current is12C6+, energy 80MeV/u, dose of radiation is
50GY, radiated time 5min, the lactobacillus plantarum after irradiation obtains after the screening of plate streaking culture repeatedly, in October, 2018
It is deposited within 8th China General Microbiological culture presevation administrative center, deposit number is CGMCC NO.:16556, preservation address Beijing
The institute 3 of city Chaoyang District North Star West Road 1.Chinese silvergrass feed nutrient measurement after ensiling is as shown in table 1, by the side
Method corrodes fermented material from other harmful miscellaneous bacterias the pH value that can not only reduce ensiling material after Chinese silvergrass progress ensiling,
Effective protection ensilage;And containing for soluble-carbohydrate and protein in Chinese silvergrass feed can also be improved after fermentation
Amount reduces ammonia nitrogen content, effectively prevents the degradation of protein in feed, protect the loss of nutriment, reduce feeding
The content of neutral detergent fiber and acid detergent fiber in material, improves the nutritional quality of feed, increases the feeding valence of feed
Value;The content for increasing organic acid content in feed, especially lactic acid simultaneously, improves the palatability of feed, improves the mouth of feed
Sense and nutrient absorption value.
Chinese silvergrass Silage Quality under 1 microbial action of table
By measurement addition charcoal and salt tolerant Chinese silvergrass plantation front and back salt affected soil items physical and chemical index, soil after discovery improvement
Earth salt content significantly reduces, and the soil organism and fertility step up (Fig. 1).It is found by high-flux sequence, added biology
The structure of soil microbial community in the salt-soda soil after charcoal and plantation salt tolerant Chinese silvergrass improvement significantly changes (figure before relatively improveing
2), these results indicate that charcoal improves and salt tolerant Chinese silvergrass is combined to plant the soil physical and chemical property that can significantly improve salt-soda soil, this
Mainly realized by the structure of community and activity that improve edaphon.
While above-described embodiment is carried out, using traditional ardealite and desulfurized gypsum to the scattered angle beach saline land of Huang into
Control improved test is gone, the usage amount of ardealite and desulfurized gypsum is 22500-37500kg/hm2;After the test, to three
Kind improved results are compared, as shown in table 2.
The comparison of test results that 2. 3 kinds of methods of table improve yellow triangle beach saline land