CN1087588C - hybrid rice breeding method - Google Patents
hybrid rice breeding method Download PDFInfo
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- CN1087588C CN1087588C CN98100613A CN98100613A CN1087588C CN 1087588 C CN1087588 C CN 1087588C CN 98100613 A CN98100613 A CN 98100613A CN 98100613 A CN98100613 A CN 98100613A CN 1087588 C CN1087588 C CN 1087588C
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- 235000009566 rice Nutrition 0.000 title claims abstract description 25
- 238000009395 breeding Methods 0.000 title description 9
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- 241000209094 Oryza Species 0.000 claims abstract description 25
- 241000196324 Embryophyta Species 0.000 claims abstract description 16
- 238000011084 recovery Methods 0.000 claims abstract description 4
- 241000746966 Zizania Species 0.000 claims description 6
- 235000002636 Zizania aquatica Nutrition 0.000 claims description 6
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- 240000008467 Oryza sativa Japonica Group Species 0.000 claims description 2
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- 208000035240 Disease Resistance Diseases 0.000 abstract description 3
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- 235000021329 brown rice Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
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- 230000008774 maternal effect Effects 0.000 description 2
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- 230000005070 ripening Effects 0.000 description 2
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
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Abstract
The present invention belongs to the field of heterosis utilization. The invention is characterized in that sterile line Chuanya 873A is taken as a female parent, and a recovery line CDR435 is taken as a male parent to combine hybrid combination Yayou 435. The combination is sown at the bottom of three months (two-stage seedling with mulching film) in Chengdu area, the whole growth period is about 155 days, the growth period is 3-5 days longer than Shanyou 63, the seedling period is vigorous, the leaf color is dark, the plant height is 112 cm, the tillering is moderate, the plant type is tight and moderate, and the spike layer is regular. Good color change in later period, green yellow rice stem, strong lodging resistance and good disease resistance. The rice has more ears and large grains, each ear has an average grain diameter of 212 grains, the setting rate is 82.1 percent, the thousand grain weight is 25.2 g, the roughness yield is 79.8 percent, the rice grains are semitransparent, the taste is good, the rice blast resistance is realized, the adaptability is wide, and the rice can be planted and popularized in high-yield areas of Sichuan and southern rice areas.
Description
Technical field:
The invention belongs to the heterosis utilization field.Specifically, the present invention relates to the selection and the corresponding propagation seed making technology of sterile line of hybridized rice and combination thereof.
Background technology:
Paddy rice is one of China's staple food crop, has bigger yield potential, and the research of hybrid rice breaks through and makes yield potential become possibility.Since China's large tracts of land was promoted hybrid rice, China's rice yield rose significantly.But belong to use of advantage between kind owing to produce the hybrid combination overwhelming majority who goes up use at present, cause output to fluctuate always, do not have new breakthrough.Along with agricultural to high yield, high-quality, direction develops the raising with the level of production efficiently, the hybrid combination of the high yield that plant in the suitable southern rice district of seed selection, disease-resistant, high-quality has become a great research topic in the breeding.
Summary of the invention:
The objective of the invention is to solve the problems referred to above in the hybrid rice production of southern rice district, a kind of high-yield hybrid paddy rice combination and high yield breeding and seeding technique are provided, produce strong advantage hybrid seed.The present invention also aims to select and problem such as disease-resistant, anti-ly fall, wide adaptability, plant and leaf shape is good, coordinate force is high subspecies three be, and be single to solve in the present production combination, and the male sterile line hereditary basis is narrow.
A kind of hybrid rice high yield provided by the invention, disease-resistant, the seed selection of good quality male sterile line and breeding and the producing method for seed of crossbreed thereof, it is characterized in that with the inferior 873A in male sterile line river be female parent, recovery is that CDR 435 is male parent, and assembly goes out high yield, disease-resistant, strong advantage hybrid combination Yayou 435 of fine quality.
The present invention is realized by following steps:
1, be male parent with japonica rice variety 02428 with wide compatibility gene, filial generation (intermediate materials) 959B of long medicine wild rice and Liuzhou wild rice is a hybridization of female parent, F2 is for isolating sterile strain and can educating two types of strains, and the fertility abortion of its sterile strain is thorough, does not dye through microscopy.And the F2 of blue or green B * 02428 early of association is normally solid for full recovery, select elite seedling that it is solid normally, plant and leaf shape is good and the paired top-cross of sterile strain individual plant that 959B * separate 02428 back, continuous multi-generation is backcrossed according to qualifications, through backcrossing according to qualifications of continuous 10 generations (F10), bred high yield, of fine qualityly, disease-resistant anti-fallen, sturdy, the big panicle many grains per panicle of cane, the high stable fertility of stigma exposing ratio, plant and leaf shape is good, the inferior 873A in the high-combining ability male sterile line river of wide adaptability.
2, be that female parent and self-fertile new restorer line CDR435 are male parent with the inferior 873A in this male sterile line river, assembly goes out high yield, strong advantage hybrid combination Yayou 435 disease-resistant, of fine quality.
Advantage of the present invention and good effect are:
1, has good biological property: inferior 873A in the river that the present invention breeds and crossbreed Yayou 435 thereof, middle blast resisting, Mi Zhizhong last (being better than Shanyou 63).Well developed root system, and slightly grow, being easy to absorb in a large number nutrition, the thick shape of cane is tough and tensile, and it is medium to tiller, and blade is thick straight, proper height, the ripe phase is suitable, and photosynthetic usefulness is good, and it is smooth to reach big source, storehouse foot, and lodging resistance is strong, and ripe look of later stage is good, big panicle many grains per panicle, ripening rate height.
2, wide adaptability: inferior 873A in the river that the present invention breeds and crossbreed Yayou 435 are insensitive to the light temperature, general do one season semilate rice plant, date of seeding, is identical with Shanyou 63, add disease-resistant, strong stress resistance, therefore the scope that adapts to plantation is wide, can be at plantation of the area of southern medium rice district Shanyou 63 plantation and crossbreeding.
3, be easy to strain breeding and seed breeding, with the obvious advantage: the production of hybrid seeds and F1 crossbreed output height, inferior 873A breeding in parent river and the general per mu yield 180-200 of hybrid seed yield kilogram.Comparison is high more than 5% according to Shanyou 63.(Asia, river 873 * CDR435): 603.3 kilograms of per mu yields are tested to the crossbreed Yayou 435 by this institute through nineteen ninety-five, and comparison is according to Shanyou 63 volume increase 16.3%; Test in 1996,625.3 kilograms of per mu yields, comparison is according to Shanyou 63 volume increase 15.1%; Da Qu in 1996 is to 630.0 kilograms of the per mu yields of multiple spot test when, and comparison is according to Shanyou 63 volume increase 18.0%.
Description of drawings
Fig. 1 is a breeding line spectrogram of the present invention
The embodiment of invention:
One, parental source and feature thereof:
After river of the present invention inferior 873A system selected non-governor's medicine wild rice and the hybridization of Liuzhou wild rice for use, taking turns choosing that 12 parent's convergent crosses form through 9 was 12968 for maternal, again with the Sichuan cultivation early rice hybridization to have bred column cap be that 959B is as female parent; Be paternal hybrid with good wide affine parent 02428 again, this parent is the high light efficiency kind 02428 (shallow lake rice/Yunnan crab paddy is drawn in the Chongming) of the Cui Ji of genetics and physiology research institute of academy of agricultural sciences, Jiangsu woods seed selection.Observe to identify through single-strain planting, screen its disease-resistant strong stress resistance, in the F2 generation in the good sterile strain of plant type and association blue or green B * 02428 early, can be educated strain and be carried out test cross and backcross.Combine male sterile line A and corresponding maintenance line B, when separating male sterile line, carry out disease resistance and identify and combining ability test.Identify that according to resistance combining ability test result and field performance were backcrossed according to qualifications continuously, isozygotied through 11 generations, bred disease-resistant strong stress resistance, wide adaptability, plant and leaf shape are good, the inferior 873A in the male sterile line river of the high yield coordinate force of big panicle many grains per panicle, and this male sterile line is that a comprehensive proterties is good, big panicle many grains per panicle, the stigma exposing ratio height, flowering habit is good, and the ripe phase is suitable, the high yield high-combining ability of proper height is the region between the heart and the diaphragm system not, and accompanying drawing is seen in its source and seed selection.
The feature of the inferior 873A in river: be intermediate form but (half Xian, half round-grained rice), Chengdu sowing on May 6, is given birth to 110 days phases to earing 80 days entirely from sowing.Production of hybrid seeds sowing in the first tenday period of a month in May, its heading and accent difference phase are undertaken by hilllock 46A.95 centimetres of this male sterile line plant heights, 18.1 centimetres of spike lengths, 147.0 of every fringe grains per panicles, stigma exposing ratio 70.41%, two exposing accounts for 36.78%, and different friendship habit is good, and is normal during flower.Its sterile degree and sterile plant rate all reach 100%.Seedling stage, growing way was prosperous, and plant strain growth is neat, the thick shape of cane, and stem blade 14 leaves, the bran point is coloured, there is short awns on the grain top, and the fringe layer is neat, and quality better, brown rice yield are 79.5%, middle blast resisting, well developed root system, the later stage annesl is good, and the yellow bar green grass or young crops of paddy, wide adaptability can be in the breeding of southern rice district and the production of hybrid seeds.
With the inferior 873A in river serves as maternal and self-fertile new restorer line " CDR435 " be a male parent, " Yayou 435 " of combo have high yield, disease-resistant, resist, characteristics such as big panicle many grains per panicle, fecundity are good, wide adaptability.
" Yayou 435 " feature: sowing (two sections seedlings of mulch film) is about the 155 days time of infertility at the end of three months for the area, Chengdu, and than the long 3-5 of Shanyou 63 days, seedling stage, growing way was prosperous, the leaf look darker, 112 centimetres of plant heights, it is medium to tiller, and plant type is tightly loose moderate, the fringe layer is neat, the later stage annesl is good, the yellow bar green grass or young crops of paddy, and cane is sturdy, lodging resistant force is strong, and disease resistance is good.Big panicle many grains per panicle, every fringe average 212 of grains, ripening rate 82.1%, thousand kernel weight 25.2 gram, brown rice yield is 79.8%, the grain of rice is translucent, food flavor is good, middle blast resisting, wide adaptability can be in Sichuan and southern rice district prolific area plantation popularization.
Claims (1)
1, the producing method for seed of a kind of hybrid rice is characterized in that the japonica rice variety 02428 to have wide compatibility gene is a male parent, and filial generation (intermediate materials) 959B of long medicine wild rice and Liuzhou wild rice is a hybridization of female parent, isolates sterile strain from F2 generation; Select solid normal individual plant and the paired top-cross of sterile strain individual plant that 959B * separate 02428 back generation from the F2 that assists blue or green B * 02428 early, breed male sterile line (the inferior 873A in river) through backcrossing according to qualifications of continuous 10 generations (F10); (the inferior 873A in river) is female parent with male sterile line, and recovery is that CDR 435 is male parent, assembly hybrid combination.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98100613A CN1087588C (en) | 1998-02-13 | 1998-02-13 | hybrid rice breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98100613A CN1087588C (en) | 1998-02-13 | 1998-02-13 | hybrid rice breeding method |
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| Publication Number | Publication Date |
|---|---|
| CN1187935A CN1187935A (en) | 1998-07-22 |
| CN1087588C true CN1087588C (en) | 2002-07-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN98100613A Expired - Fee Related CN1087588C (en) | 1998-02-13 | 1998-02-13 | hybrid rice breeding method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240277B (en) * | 2007-02-09 | 2011-07-20 | 中国农业科学院植物保护研究所 | PCR detection method of transgene paddy strain Bt Shanyou 63 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094036C (en) * | 1998-06-05 | 2002-11-13 | 福建农业大学 | Selective breeding method for ear covering free male-sterile line paddy rice |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044201A (en) * | 1990-03-09 | 1990-08-01 | 江苏省农业科学院粮食作物研究所 | Hybrids of Indica and Japonica rice---inferior excellent No. two breeding method |
| CN1159878A (en) * | 1995-11-23 | 1997-09-24 | 菲思卡斯日用消费品公司 | Pivoted hand tool |
-
1998
- 1998-02-13 CN CN98100613A patent/CN1087588C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1044201A (en) * | 1990-03-09 | 1990-08-01 | 江苏省农业科学院粮食作物研究所 | Hybrids of Indica and Japonica rice---inferior excellent No. two breeding method |
| CN1159878A (en) * | 1995-11-23 | 1997-09-24 | 菲思卡斯日用消费品公司 | Pivoted hand tool |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101240277B (en) * | 2007-02-09 | 2011-07-20 | 中国农业科学院植物保护研究所 | PCR detection method of transgene paddy strain Bt Shanyou 63 |
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| Publication number | Publication date |
|---|---|
| CN1187935A (en) | 1998-07-22 |
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