CN106359237A - Marine shellfish biological physiological experiment device and method based on community level - Google Patents
Marine shellfish biological physiological experiment device and method based on community level Download PDFInfo
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- CN106359237A CN106359237A CN201610956599.5A CN201610956599A CN106359237A CN 106359237 A CN106359237 A CN 106359237A CN 201610956599 A CN201610956599 A CN 201610956599A CN 106359237 A CN106359237 A CN 106359237A
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- 238000002474 experimental method Methods 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 16
- 235000015170 shellfish Nutrition 0.000 title abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 68
- 239000013535 sea water Substances 0.000 claims abstract description 15
- 230000035479 physiological effects, processes and functions Effects 0.000 claims description 33
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 12
- 239000001301 oxygen Substances 0.000 claims description 12
- 229910052760 oxygen Inorganic materials 0.000 claims description 12
- 230000000035 biogenic effect Effects 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 230000002503 metabolic effect Effects 0.000 claims description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 6
- 238000009360 aquaculture Methods 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 238000009304 pastoral farming Methods 0.000 claims description 6
- 229910000831 Steel Inorganic materials 0.000 claims description 5
- 244000144974 aquaculture Species 0.000 claims description 5
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 5
- 239000010959 steel Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 4
- 229920005439 Perspex® Polymers 0.000 claims description 3
- 229920001617 Vinyon Polymers 0.000 claims description 3
- 229910021529 ammonia Inorganic materials 0.000 claims description 3
- 238000001354 calcination Methods 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 claims description 3
- 235000013372 meat Nutrition 0.000 claims description 3
- 239000002846 particulate organic matter Substances 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 238000004062 sedimentation Methods 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 2
- 238000007789 sealing Methods 0.000 abstract description 4
- 230000037406 food intake Effects 0.000 abstract 1
- 230000004060 metabolic process Effects 0.000 abstract 1
- 238000011160 research Methods 0.000 description 7
- UELITFHSCLAHKR-UHFFFAOYSA-N acibenzolar-S-methyl Chemical compound CSC(=O)C1=CC=CC2=C1SN=N2 UELITFHSCLAHKR-UHFFFAOYSA-N 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 230000001360 synchronised effect Effects 0.000 description 4
- 230000002706 hydrostatic effect Effects 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 229920005479 Lucite® Polymers 0.000 description 2
- 241000237536 Mytilus edulis Species 0.000 description 2
- 241000902900 cellular organisms Species 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 235000020638 mussel Nutrition 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000001228 trophic effect Effects 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241000237509 Patinopecten sp. Species 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 235000020637 scallop Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Marine Sciences & Fisheries (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to the technical field of marine organism physiological experiments, in particular to a marine shellfish biological physiological experiment device based on community level and a use method thereof, wherein the experimental device comprises a transparent upper barrel body, a funnel, a supporting barrel body and a collecting bottle, a sealing cover is arranged at the top of the transparent upper barrel body, an inner hanging rod for hanging an experimental organism community is arranged in the transparent upper barrel body, a water inlet, an external water pump and a water control valve are arranged on one side barrel wall, and a water outlet is arranged on the other side barrel wall; the middle part of the supporting barrel body is provided with a cylindrical opening, a collecting bottle is placed in the cylindrical opening, and the lower end pipe of the funnel extends into the collecting bottle. The invention simulates the conditions of natural seawater temperature, flow velocity and the like through the experimental device, and utilizes the large-volume container to carry out ingestion physiological experiment and metabolism physiological experiment of the marine shellfish biological community, thereby reducing the experimental links, and avoiding the error existing when the biological individual experimental result is popularized to the whole ecological system while keeping the biological community complete.
Description
Technical field
The present invention relates to marine organisms Physiological Experiment technical field is and in particular to a kind of seashells based on community level
Biological physiology experimental provision and experimental technique.
Background technology
Ocean Filter feeding bivalves are the important component parts of living resources structure in marine ecosystems, shellfish is ingested,
The research of the physiological energeticses such as assimilation, breathing, excretion, can provide necessary data supporting for assessing the carrying capacity in sea area,
For inquiring into effect in aquatic ecosystem for the shellfish and function has important theory significance.
At present Filter feeding bivalves ingest and the research method of metabolic physiology mainly includes hydrostatic method and simulated field flowing water
Channel process, and research aspect is based primarily upon individual level.Hydrostatic method mainly adopts the container of certain volume, by manual control water body
Temperature, add bait realizing, major defect is that container size is typically small, the feed source of bioorganism be difficult to
Water body actual food concentrations in off-lying sea sea area are synchronous, and experimental result accuracy is relatively low；Simulated field flowing water method is widely used at present
Research method, this method treatment in accordance with local conditions designs secondary water supply system, bioorganism individuality is individually positioned in gutter channel,
Control the seawater velocity of gutter channel by isocon, according to the trophic behavior that the experimental result research experiment before and after experiment is biological,
After feeding experiment terminates, each gutter channel is closed, carries out metabolic physiology experiment, this method is preferably synchronized the water in sea area
Temperature, food concentrations, but result of study is only understanding aquaculture organism individuality physiological activity and provides reference.
Recognize the continuous expansion in the marine ecosystems visual field with people, increasing researcher tends to from entirely giving birth to
The aspect of state system recognizes the ecological functions of shellfish.However, due in aquaculture organism local environment between biome, cultivating life
The interaction existing between thing individuality, the assessment impact to ecosystem for the shellfish is not the simple of individual level result of study
Superposition, if the experimental result of individual level is extrapolated to during whole ecosystem can produce very big deviation.In contrast mussel
Find in individual level and the research of community level physiological activity, the filtration rate of individual level and consumption rate are significantly higher than group's water
Flat.In this context, research and development are a set of is particularly important based on the Physiological Experiment system of community level.
Content of the invention
According to the deficiencies in the prior art, the invention provides a kind of seashells biological physiology experiment based on community level
Device and experimental technique, to solve problems of the prior art:
A kind of seashells biological physiology experimental provision based on community level of the present invention, including transparent upper barrel body, leakage
Bucket, support staving and receiving flask, the upper end quarrel of described funnel is connected to the bottom of transparent upper barrel body, supports staving to pass through first
Flange is connected to the bottom of transparent upper barrel body, and the top of transparent upper barrel body connects closure, transparent upper barrel body by second flange
Inside be provided with interior peg for suspending bioorganism group in midair, the bottom of the side bucket wall of transparent upper barrel body be provided with water inlet,
Outer water pump and water control valve, the upper and lower of opposite side bucket wall is respectively equipped with outlet；The described middle part supporting staving opens up
There is cylindricality mouth, in cylindricality mouth, place receiving flask, the lower end pipe of funnel stretches into described receiving flask.
Preferably, the material of described transparent upper barrel body is perspex steel.Lucite steel drum staving transparent it is ensured that being
The light transmission of system and the vigor situation being easy to Real Time Observation bioorganism.
Preferably, described transparent upper barrel body and a diameter of 50cm supporting staving, the height of transparent upper barrel body is 80cm.
Compared with the device carrying out bion experiment, the staving capacity of the present invention is big, and the biomass that can accommodate is many, conveniently carries out
Biome is tested, and can also be suspended at the differing heights of transparent upper barrel body by different biomes, simultaneously to many simultaneously
Group biome is tested.
Preferably, a diameter of 10cm of described cylindricality mouth, highly for 20cm, for placing receiving flask.
Preferably, the material of described receiving flask is vinyon, a diameter of 6cm of described receiving flask, be highly
18cm.The overall size of receiving flask is less than cylindricality mouth, is easy to place.
Preferably, a diameter of 6cm of the lower end pipe of described funnel, highly for 18cm.The bottom of lower end pipe is provided with and receives
The screw thread that collection bottle bottleneck matches.
Preferably, described closure is provided with the circular hole for placing measuring instrument, a diameter of 5cm of described circular hole.
Preferably, multiple screws are evenly arranged with respectively on described first flange and second flange, first flange with close
Between capping, second flange and support staving between be all tightly connected by fastening screw and rubber sheet gasket, make experimental provision whole
Body keeps sealing.
Preferably, described interior peg is arranged at 5cm below transparent upper barrel body top.Convenient suspension and taking-up experiment life
Thing group, every group of biological string bag etc. that passes through is grouped as a group, will be a certain in experimental provision for each group's integral hanging
Position, while maintaining biocenological complete, it is to avoid bion experimental result is generalized to deposits during whole ecosystem
Error.
The present invention is comprised the steps: based on the seashells biological physiology experimental technique of community level
(1) bioorganism group is suspended on interior peg, the outer water pump of water inlet supplies water, and water control valve controls water inlet water
Amount, optionally opens or closes two outlets, simulated seawater flowing velocity；
(2) after bioorganism group adapts to 1-2h under flowing water state, empty receiving flask, start Feeding Physiology experiment；Real
Test lasting 10-12h, every 3h measures the suspended particulate substance content of a water inlet, particulate organic matter content；After experiment terminates, will
Bucket maritime interior waters emptying, takes off receiving flask, and after the biogenic deposits sedimentation in receiving flask, collection, drying, calcination, measure deposit
Gross dry weight and content of organics, calculate biocenological filtration rate and grazing rate using biogenic deposit method；
(3) after clean receiving flask being reinstalled, the full sea water of pump, aquaculture organism adapts to 1-2h under flowing water state
Afterwards, start metabolic physiology experiment, entery and delivery port Close All, measured using ysi multi-parameter water quality detector and rdo dissolved oxygen meter
Initial seawater temperature, salinity, dissolved oxygen, water sampling measures ammonia-nitrogen content；
(4) start timing, 2-3h closed by experimental provision, can be through in transparent upper barrel body observation experiment device during closing
Biologos situation；
(5) open top closure, the ocean temperature at the end of determination experiment, salinity, dissolved oxygen, take in transparent upper barrel body
At upper, middle and lower three, the water sample of position, as aggregate sample, measures ammonia-nitrogen content；
(6) by bioorganism community classification, collect in sealed bag, take back laboratory post-drying, separating shell and meat, weigh,
According to the dry weight of water body, Excreta experimental data and bioorganism before and after experiment, calculate colony's filtration rate, grazing rate, suction
Produce effects a series of physical signs parameter such as rate, consumption rate, ammonia N-excretion rates.
The seashells biological physiology experimental provision based on community level of the present invention and experimental technique, have excellent as follows
Point:
(1) compared with hydrostatic method, the experimental provision of the present invention can be built and the environment such as nature seawater water temperature, food concentrations ginseng
The consistent experiment condition of number, actual food concentrations are synchronous with natural waterses water body, and experimental result accuracy is higher；
(2) compared with simulated field flowing water method, the experimental provision of the present invention not only can control water inlet and go out water speed, mould
Intend natural waterses seawater velocity, according to the trophic behavior that the experimental result research experiment before and after experiment is biological, can also be by each
Seashells biome is studied as overall inserting in experimental provision, rather than carries out reality only for each bion
Test；
(3) present invention can close water inlet and water outlet, experimental provision closed after Feeding Physiology experiment terminates,
And proceed metabolic physiology experiment, reduce experiment link, complete Feeding Physiology experiment and metabolic physiology experiment simultaneously；
(4) the biological design of the seashells based on community level for the present invention, while maintaining biocenological complete, keeps away
Exempt from bion experimental result and be generalized to the error existing during whole ecosystem.
Brief description
Fig. 1 is the overall structure diagram of the seashells biological physiology experimental provision based on community level of the present invention；
Fig. 2 is that the sealing cover structure of the seashells biological physiology experimental provision based on community level of the present invention is illustrated
Figure；
Fig. 3 is the seashells biological physiology experimental technique flow chart based on community level of the present invention.
In figure, 1: transparent upper barrel body, 2: funnel, 3: support staving, 4: receiving flask, 5: first flange, 6: second flange,
11: closure, 111: circular hole, 12: interior peg, 13: water inlet, 14: outlet, 21: upper end quarrel, 22: lower end pipe, 31: post
Shape mouth.
Specific embodiment
Below in conjunction with drawings and Examples, the invention will be further described.
Embodiment 1:
As shown in Figure 1, 2, the seashells biological physiology experimental provision based on community level of the present embodiment, including transparent
Upper barrel body 1, funnel 2, support staving 3 and receiving flask 4, the upper end quarrel 21 of described funnel 2 is connected under transparent upper barrel body 1
Portion, supports staving 3 to be connected to the bottom of transparent upper barrel body 1 by first flange 5, the second method is passed through at the top of transparent upper barrel body 1
Blue 6 connection closures 11, the inside of transparent upper barrel body 1 is provided with the interior peg 12 for suspending bioorganism group in midair, interior peg 12
On can hang one or more groups of biomes, carry out one group or carry out multigroup experiment simultaneously.Different seashells biotas
Fall, can be using different hangs, the such as biome of mussel, Concha Ostreae etc., directly hung on interior peg, right
Utilize the biology of cylinder mould cultivation in scallop etc., its aquiculture mesh cage can be hung on interior peg.
The bottom of the side bucket wall of transparent upper barrel body 1 is provided with water inlet 13, outer water pump and water control valve, opposite side bucket wall
Upper and lower is respectively equipped with outlet 14；The experiment consistent with ambient parameters such as nature seawater water temperature, food concentrations can be built
Condition, actual food concentrations are synchronous with natural waterses water body, and experimental result accuracy is higher.
The described middle part supporting staving 3 offers cylindricality mouth 31, places receiving flask 4, the lower end pipe of funnel 2 in cylindricality mouth 31
22 stretch into described receiving flask 4.Receiving flask is used for collecting seashells biogenic deposits, is used for mensure of testing.
The material of described transparent upper barrel body 1 is perspex steel.Lucite steel drum staving transparent it is ensured that the printing opacity of system
Property and the vigor situation being easy to Real Time Observation bioorganism.
Described transparent upper barrel body 1 and a diameter of 50cm supporting staving 3, the height of transparent upper barrel body 1 is 80cm.With carry out
The device of bion experiment is compared, and the staving capacity of the present invention is big, and the biomass that can accommodate is many, conveniently carries out biota
Implement and test, different biomes can also be suspended at the differing heights of transparent upper barrel body, simultaneously to multigroup biology simultaneously
Group is tested.
A diameter of 10cm of described cylindricality mouth 31, highly for 20cm, for placing receiving flask 4.
The material of described receiving flask 4 is vinyon, and a diameter of 6cm of described receiving flask 4, highly for 18cm.Collect
The overall size of bottle 4 is less than cylindricality mouth, is easy to place.
A diameter of 6cm of the lower end pipe 22 of described funnel 2, highly for 18cm.The bottom of lower end pipe 22 is provided with and receiving flask 4
The screw thread that bottleneck matches.
Described closure 11 is provided with the circular hole 111 for placing measuring instrument, a diameter of 5cm of described circular hole 111.Survey
Measuring appratus include for measurement temperature, salinity, the ysiproplus multi-parameter water quality detector of ph and are used for measuring dissolved oxygen
Rdo dissolved oxygen meter.
It is evenly arranged with multiple screws, first flange 5 and closure 11 respectively in described first flange 5 and second flange 6
Between, second flange 6 and supporting all be tightly connected by fastening screw and rubber sheet gasket between staving 3, make experimental provision integrally
Keep sealing.
Described interior peg 12 is arranged at 5cm below transparent upper barrel body 1 top.Convenient suspension and taking-up bioorganism group
Fall, every group of biological string bag etc. that passes through is grouped as a group, by each group's integral hanging in a certain position of experimental provision,
While maintaining biocenological complete, it is to avoid bion experimental result is generalized to the mistake existing during whole ecosystem
Difference.
Embodiment 2:
As shown in figure 3, the present embodiment include walking as follows based on the seashells biological physiology experimental technique of community level
Rapid:
S1: bioorganism group is suspended on interior peg, the outer water pump of water inlet supplies water, and water control valve controls water inlet water
Amount, optionally opens or closes two outlets, simulated seawater flowing velocity, is sufficiently mixed for guarantee staving interior edema stream, excellent
First open top outlet；
After s2: bioorganism group adapts to 1-2h under flowing water state, empty receiving flask, start Feeding Physiology experiment；Real
Test lasting 10-12h, every 3h measures the suspended particulate substance content of a water inlet, particulate organic matter content；After experiment terminates, will
Bucket maritime interior waters emptying, takes off receiving flask, and after the biogenic deposits sedimentation in receiving flask, collection, drying, calcination, measure deposit
Gross dry weight and content of organics, calculate biocenological filtration rate and grazing rate using biogenic deposit method；
S3: after clean receiving flask is reinstalled, the full sea water of pump, aquaculture organism adapts to 1-2h under flowing water state
Afterwards, start metabolic physiology experiment, entery and delivery port Close All, measured using ysi multi-parameter water quality detector and rdo dissolved oxygen meter
Initial seawater temperature, salinity, dissolved oxygen, water sampling measures ammonia-nitrogen content；
S4: start timing, 2-3h closed by experimental provision, can be through in transparent upper barrel body observation experiment device during closing
Biologos situation；
S5: open top closure, the ocean temperature at the end of determination experiment, salinity, dissolved oxygen, take in transparent upper barrel body
At upper, middle and lower three, the water sample of position, as aggregate sample, measures ammonia-nitrogen content；
S6: bioorganism community classification is collected in sealed bag, takes back laboratory post-drying, separating shell and meat, weigh,
According to the dry weight of water body, Excreta experimental data and bioorganism before and after experiment, calculate colony's filtration rate, grazing rate, suction
Produce effects a series of physical signs parameter such as rate, consumption rate, ammonia N-excretion rates.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement made etc., should be included within the scope of the present invention.
Claims (10)
1. a kind of seashells biological physiology experimental provision based on community level it is characterised in that:
Including transparent upper barrel body, funnel, support staving and receiving flask, the upper end quarrel of described funnel is connected to transparent upper barrel body
Bottom, supports staving to be connected to the bottom of transparent upper barrel body by first flange；Second flange is passed through at the top of transparent upper barrel body
Connect closure, the inside of transparent upper barrel body is provided with the interior peg for suspending bioorganism group in midair；The side of transparent upper barrel body
The bottom of bucket wall is provided with water inlet, outer water pump and water control valve, and the upper and lower of opposite side bucket wall is respectively equipped with outlet；Institute
State and support the middle part of staving to offer cylindricality mouth, in cylindricality mouth, place receiving flask, the lower end pipe of funnel stretches into described receiving flask.
2. as claimed in claim 1 a kind of seashells biological physiology experimental provision based on community level it is characterised in that:
The material of described transparent upper barrel body is perspex steel.
3. as claimed in claim 1 a kind of seashells biological physiology experimental provision based on community level it is characterised in that:
Described transparent upper barrel body and a diameter of 50cm supporting staving, the height of transparent upper barrel body is 80cm.
4. as claimed in claim 1 a kind of seashells biological physiology experimental provision based on community level it is characterised in that:
A diameter of 10cm of described cylindricality mouth, highly for 20cm.
5. as claimed in claim 1 a kind of seashells biological physiology experimental provision based on community level it is characterised in that:
The material of described receiving flask is vinyon, and a diameter of 6cm of described receiving flask, highly for 18cm.
6. as claimed in claim 1 a kind of seashells biological physiology experimental provision based on community level it is characterised in that:
A diameter of 6cm of the lower end pipe of described funnel, highly for 18cm.
7. as claimed in claim 1 a kind of seashells biological physiology experimental provision based on community level it is characterised in that:
Described closure is provided with the circular hole for placing measuring instrument, a diameter of 5cm of described circular hole, and described measuring instrument includes using
In measurement temperature, salinity, the ysiproplus multi-parameter water quality detector of ph and the rdo dissolved oxygen meter for measuring dissolved oxygen.
8. as claimed in claim 1 a kind of seashells biological physiology experimental provision based on community level it is characterised in that:
It is evenly arranged with multiple screws respectively, between first flange and closure, second flange in described first flange and second flange
And support and be all tightly connected by fastening screw and rubber sheet gasket between staving.
9. as claimed in claim 1 a kind of seashells biological physiology experimental provision based on community level it is characterised in that:
Described interior peg is arranged at 5cm below transparent upper barrel body top.
10. a kind of seashells biological physiology experimental technique based on community level is it is characterised in that comprise the following steps:
S1: bioorganism group is suspended on interior suspension member, the outer water pump of water inlet supplies water, and water control valve controls amount of inlet water, choosing
Selecting property open or close two outlets, simulated seawater flowing velocity；
After s2: bioorganism group adapts to 1-2h under flowing water state, empty receiving flask, start Feeding Physiology experiment；Experiment is held
Continuous 10-12h, every 3h measures the suspended particulate substance content of a water inlet, particulate organic matter content；After experiment terminates, by bucket
Sea water empties, and takes off receiving flask, collection, drying, calcination after the biogenic deposits sedimentation in receiving flask, measures deposit and always does
Weight and content of organics, calculate biocenological filtration rate and grazing rate using biogenic deposit method；
S3: after clean receiving flask is reinstalled, the full sea water of pump, after aquaculture organism adapts to 1-2h under flowing water state, open
Beginning metabolic physiology is tested, entery and delivery port Close All, measures initial seawater temperature, salinity, dissolved oxygen, and water sampling measures ammonia nitrogen
Content；
S4: start timing, 2-3h closed by experimental provision, can be through the life in transparent upper barrel body observation experiment device during closing
Thing vigor situation；
S5: open top closure, the ocean temperature at the end of determination experiment, salinity, dissolved oxygen, take in transparent upper barrel body,
In, at lower three position water sample as aggregate sample, measure ammonia-nitrogen content；
S6: bioorganism community classification is collected in sealed bag, takes back laboratory post-drying, separating shell and meat, weigh, according to
The dry weight of the water body before and after experiment, Excreta experimental data and bioorganism, calculates colony's filtration rate, grazing rate, absorbs effect
A series of physical signs parameter such as rate, consumption rate, ammonia N-excretion rates.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107515234A (en) * | 2017-08-31 | 2017-12-26 | 鲁东大学 | A device and method for measuring photosynthetic intensity of submerged plants |
| CN109275560A (en) * | 2018-12-04 | 2019-01-29 | 中国水产科学研究院黄海水产研究所 | A kind of large ocean algae is acidified the system and research method of adaptation Journal of Sex Research for a long time |
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| CN109275560B (en) * | 2018-12-04 | 2021-02-12 | 中国水产科学研究院黄海水产研究所 | System and method for researching long-term acidification adaptability of large marine algae |
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