CN105319282A - Content measurement method of compound amino acid (15) dipeptide (2) injection - Google Patents
Content measurement method of compound amino acid (15) dipeptide (2) injection Download PDFInfo
- Publication number
- CN105319282A CN105319282A CN201410279676.9A CN201410279676A CN105319282A CN 105319282 A CN105319282 A CN 105319282A CN 201410279676 A CN201410279676 A CN 201410279676A CN 105319282 A CN105319282 A CN 105319282A
- Authority
- CN
- China
- Prior art keywords
- elution
- amino acid
- carried out
- citric acid
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002347 injection Methods 0.000 title abstract description 4
- 239000007924 injection Substances 0.000 title abstract description 4
- -1 compound amino acid Chemical class 0.000 title abstract 6
- 108010016626 Dipeptides Proteins 0.000 title abstract 4
- 238000000691 measurement method Methods 0.000 title abstract 3
- 150000001413 amino acids Chemical class 0.000 claims abstract description 36
- 239000000872 buffer Substances 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 19
- 238000010828 elution Methods 0.000 claims abstract description 11
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000003456 ion exchange resin Substances 0.000 claims abstract description 4
- 229920003303 ion-exchange polymer Polymers 0.000 claims abstract description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 72
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 28
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 28
- 239000012153 distilled water Substances 0.000 claims description 17
- 239000000243 solution Substances 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 15
- 239000011780 sodium chloride Substances 0.000 claims description 14
- 239000001509 sodium citrate Substances 0.000 claims description 14
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 14
- 239000003182 parenteral nutrition solution Substances 0.000 claims description 12
- 235000001014 amino acid Nutrition 0.000 abstract description 26
- 238000000926 separation method Methods 0.000 abstract description 23
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract description 5
- 235000004279 alanine Nutrition 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- PNMUAGGSDZXTHX-BYPYZUCNSA-N Gly-Gln Chemical compound NCC(=O)N[C@H](C(O)=O)CCC(N)=O PNMUAGGSDZXTHX-BYPYZUCNSA-N 0.000 abstract description 3
- 238000001514 detection method Methods 0.000 abstract description 3
- 108010010147 glycylglutamine Proteins 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 2
- 108010079364 N-glycylalanine Proteins 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 9
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 5
- 239000011976 maleic acid Substances 0.000 description 5
- 238000009795 derivation Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 239000001530 fumaric acid Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000005374 membrane filtration Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 3
- IEFJWDNGDZAYNZ-BYPYZUCNSA-N Gly-Glu Chemical compound NCC(=O)N[C@H](C(O)=O)CCC(O)=O IEFJWDNGDZAYNZ-BYPYZUCNSA-N 0.000 description 3
- 108010002311 N-glycylglutamic acid Proteins 0.000 description 3
- 229930003268 Vitamin C Natural products 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 238000013016 damping Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000019154 vitamin C Nutrition 0.000 description 3
- 239000011718 vitamin C Substances 0.000 description 3
- 239000000337 buffer salt Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001212 derivatisation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002848 electrochemical method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 241001597008 Nomeidae Species 0.000 description 1
- 238000012356 Product development Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to the field of medicine production and particularly relates to a content measurement method of a compound amino acid (15) dipeptide (2) injection. In the method, an ion exchange resin column is employed with following conditions: column temperature is 52-62 DEG C, flow quantity is 0.4 ml/min, detection wavelengths are 570 nm and 440 nm, a mobile phase is composed of buffer liquids B1, B2, B3, B4 and B5 and elution time is 60 min, wherein the elution in the 0-2.5 min is carried out with the B1 being 100%, the elution in the 2.6-5 min is carried out with the B2 being 100%, the elution in the 6.1-13.8 min is carried out with the B3 being 100%, the elution in the 13.9-29.0 min is carried out with the B4 being 100%, the elution in the 29.1-33 min is carried out with the B5 being 100%, the elution in the 33.1-34.0 min is carried out with the B2 being 100% and the elution in the 34.1-53 min is carried out with the B1 being 100%. The content measurement method achieves excellent separation between glycyl glutamine and alanine in the compound amino acid (15) dipeptide (2) injection, satisfies requirement of separation degree on different components in the sample and achieves accurate detection of the contents of fourteen amino acids and one dipeptide in the sample.
Description
Technical field
The present invention relates to pharmaceutical production field, be specifically related to a kind of amino acid (15) two peptide (2) parenteral solution content assaying method.
Background technology
Amino acid is the important substance in the fundamental structural unit of protein and bio-metabolic process, Analytical Technology of Amino Acid to protein chemistry, biological chemistry and whole life science and product development, the production management of quality control box etc. significant, be widely used in the analysis of medicine, food, health products etc. of food processing, medical and health industry.Its analysis determining method, can be divided into chemical analysis, electrochemical methods, spectrophotometric method etc. according to detection method; According to the priority of derivatization reaction, column front derivation and post-column derivation can be divided into.
In above analytical approach, the specificity of chemical analysis, electrochemical methods and spectrophotometric method is poor, can not realize several amino acids separation determination simultaneously; Post-column derivation method before and after post, although can use automatic sampler and the chromatograph joint used separation determination realizing several amino acids, deriving technology is comparatively complicated, and the cycle is longer, and derivatization conditions is wayward, and repeatability is poor, is difficult to promote.
Amino-acid analyzer can be separated and assay several amino acids simultaneously, and need not carry out before post and post-column derivation, can measure by direct injection analysis, sensitive, quick, the data precision is high; Resolution is high, simple to operate, favorable reproducibility, and peak shape is good, stable system, there is not the problem that liquid phase process generates secondary derivant, one of most widely used method in amino acid qualitative and quantitative analysis so far.
Conventional Contents of Amino Acids, what usually adopt is all the separation condition that amino-acid analyzer instrument producer itself is recommended, and comprises pH of cushioning fluid, buffer exchange time and column temperature etc.
But, when in using amino-acid analyzer to amino acid (15) two peptide (2) parenteral solution, 14 seed amino acids and 1 two peptide (Gly-Glu) carry out assay, when the separation condition using instrument itself to recommend is tested, the separating effect of the Gly-Glu wherein in sample and alanine two components is bad, do not reach the requirement of test to Component seperation degree, cause assay result precision not good.
Summary of the invention
The present invention is directed to deficiency of the prior art, a kind of amino acid (15) two peptide (2) parenteral solution content assaying method is provided.
Technical scheme of the present invention is:
A kind of amino acid (15) two peptide (2) parenteral solution content assaying method, adopt ion exchange resin column, column temperature 52-62 DEG C, flow is 0.4ml/min, determined wavelength is 570nm and 440nm, mobile phase is by buffer B 1, B2, B3, B4, B5 forms, elution time is 60 minutes, the B1 of 100% within 0-2.5 minute, is adopted to carry out wash-out, the B2 of 100% within 2.6-5 minute, is adopted to carry out wash-out, the B3 of 100% within 6.1-13.8 minute, is adopted to carry out wash-out, 13.9-29.0 the B4 of minute employing 100% carries out wash-out, 29.1-33 the B5 of minute employing 100% carries out wash-out, 33.1-34.0 the B2 of minute employing 100% carries out wash-out, 34.1-53 the B1 of minute employing 100% carries out wash-out,
Described buffer B 1 consists of 6.19g sodium citrate 2H
2o, 1MNaOH6-7mL, 5.66g sodium chloride, 19.80g citric acid H
2o, 135.0ml ethanol, 1-3g citric acid, be mixed with 1000mL solution with distilled water;
Described buffer B 2 consists of 7.74g sodium citrate 2H
2o, 1MNaOH20-22mL, 7.07g sodium chloride, 22.00g citric acid H
2o, 25.0ml ethanol, is mixed with 1000mL solution with distilled water;
Described buffer B 3 consists of 13.31g sodium citrate 2H
2o, 3.74g sodium chloride, 12.80g citric acid H
2o, 9.00ml ethanol, is mixed with 1000mL solution with distilled water;
Described buffer B 4 consists of 26.67g sodium citrate 2H
2o, 54.35g sodium chloride, 6.10g citric acid H
2o, is mixed with 1000mL solution with distilled water;
Described buffer B 5 consists of 8.00gNaOH, 100.0ml ethanol, is mixed with 1000mL solution with distilled water.
Preferably, described column temperature is 57 DEG C.
Preferably, described buffer B 1 consists of 6.19g sodium citrate 2H
2o, 1MNaOH6-7mL, 5.66g sodium chloride, 19.80g citric acid H
2o, 135.0ml ethanol, 2g citric acid, be mixed with 1000mL solution with distilled water.
The invention has the beneficial effects as follows:
Amino acid of the present invention (15) two peptide (2) parenteral solution content assaying method, achieve the good separation of glycylglutamine and alanine in amino acid (15) two peptide (2) parenteral solution, meet the requirement of test to the degree of separation of sample different component, achieve the Accurate Determining of amino acid and a kind of two peptide content in 14 in sample.
Accompanying drawing explanation
The separating spectrum that accompanying drawing 1 obtains for the embodiment of the present invention.
Embodiment
The specific embodiment of the present invention is as follows, separating spectrum as shown in Figure 1:
Adopt Hitachi L-8900 amino-acid analyzer
Test specimen: amino acid (15) two peptide (2) parenteral solution (Huaren Pharmaceutical Co., Ltd.'s production)
Reference substance: examine institute in source, lot number 140624-200805, purity 100%;
Post pressure: the post pressure of pump 1 is about 11Mpa, and pump 2 post pressure is 2.0Mpa;
Applied sample amount: 10 μ L
Adopt ion exchange resin column (purchased from Hitachi), column temperature 57 DEG C, flow is 0.4ml/min, determined wavelength is 570nm and 440nm, mobile phase is by buffer B 1, B2, B3, B4, B5 forms, elution time is 60 minutes, the B1 of 100% within 0-2.5 minute, is adopted to carry out wash-out, the B2 of 100% within 2.6-6 minute, is adopted to carry out wash-out, the B3 of 100% within 6.1-13.8 minute, is adopted to carry out wash-out, 13.9-29.0 the B4 of minute employing 100% carries out wash-out, 29.1-33 the B5 of minute employing 100% carries out wash-out, 33.1-34.0 the B2 of minute employing 100% carries out wash-out, 34.1-53 the B1 of minute employing 100% carries out wash-out,
Table 1: eluent system
| Time(min) | ％B1 | ％B2 | ％B3 | ％B4 | ％B5 |
| 0.0 | 100 | 0 | 0 | 0 | 0 |
| 2.5 | 100 | 0 | 0 | 0 | 0 |
| 2.6 | 0 | 100 | 0 | 0 | 0 |
| 6.0 | 0 | 100 | 0 | 0 | 0 |
| 6.1 | 0 | 0 | 100 | 0 | 0 |
| 13.8 | 0 | 0 | 100 | 0 | 0 |
| 13.9 | 0 | 0 | 0 | 100 | 0 |
| 29.0 | 0 | 0 | 0 | 100 | 0 |
| 29.1 | 0 | 0 | 0 | 0 | 100 |
| 33.0 | 0 | 0 | 0 | 0 | 100 |
| 33.1 | 0 | 100 | 0 | 0 | 0 |
| 34.0 | 0 | 100 | 0 | 0 | 0 |
| 34.1 | 100 | 0 | 0 | 0 | 0 |
| 53.0 | 100 | 0 | 0 | 0 | 0 |
Note: the % in table refers to percent by volume.
Described buffer B 1 consists of 6.19g sodium citrate 2H
2o, 1MNaOH6-7mL, 5.66g sodium chloride, 19.80g citric acid H
2o, 135.0ml ethanol, 2g citric acid, be mixed with 1000mL solution with distilled water;
Described buffer B 2 consists of 7.74g sodium citrate 2H
2o, 1MNaOH20-22mL, 7.07g sodium chloride, 22.00g citric acid H
2o, 25.0ml ethanol, is mixed with 1000mL solution with distilled water;
Described buffer B 3 consists of 13.31g sodium citrate 2H
2o, 3.74g sodium chloride, 12.80g citric acid H
2o, 9.00ml ethanol, is mixed with 1000mL solution with distilled water;
Described buffer B 4 consists of 26.67g sodium citrate 2H
2o, 54.35g sodium chloride, 6.10g citric acid H
2o, is mixed with 1000mL solution with distilled water;
Described buffer B 5 consists of 8.00gNaOH, 100.0ml ethanol, is mixed with 1000mL solution with distilled water.
Table 2: the preparation table of damping fluid
Result as shown in Figure 1, the good separation of glycylglutamine and alanine in amino acid (15) two peptide (2) parenteral solution, meet the requirement of test to the degree of separation of sample different component, achieve the Accurate Determining of amino acid and a kind of two peptide content in 14 in sample.
Test example:
The condition such as column temperature tested for the pH value of damping fluid, the conversion time of damping fluid and separating column has carried out a series of test, and found that separating column column temperature is 57 DEG C time, separating effect is best, as table 3.
Table 3: column temperature affects degree of separation
Under the prerequisite of the such as buffer system of table 1, the buffer salts such as citric acid, maleic acid, fumaric acid and vitamin C are added respectively in the buffer B 1 in 1000mL, when adding the citric acid of 2g amount in the buffer B 1 of 1000mL, in sample, the separation case of a component is best, other buffer salts, result is all undesirable.
In process of the test, in the buffer B 1 of 1000mL, add maleic acid in right amount, ultrasonic 5min, mixing, through 0.45 μm of membrane filtration process, stand-by.After adding the maleic acid of different amount, in sample component, the separation case of each material is as shown in the table, and the separating resulting of constituent part is undesirable, as table 4.
Table 4: add the impact of maleic acid on degree of separation in buffer B 1
Fumaric acid is added appropriate, ultrasonic 5min, mixing in the buffer B 1 of 1000mL, through 0.45 μm of membrane filtration process, stand-by.After adding the fumaric acid of different amount, in sample component, the separation case of each material is as shown in the table, and the separating resulting of constituent part is still undesirable, and when adopting this condition, the column pressure of separating column has rising trend (as table 5) simultaneously.Table 5: add the impact of fumaric acid on degree of separation in buffer B 1
Vitamin C is added appropriate, ultrasonic 5min, mixing in the buffer B 1 of 1000mL, through 0.45 μm of membrane filtration process, stand-by.After adding the maleic acid of different amount, in sample component, the separation case of each material is as shown in the table, and the separating resulting of constituent part is still undesirable, and the reappearance of sample result poor (as table 6).
Table 6: add the impact of vitamin C on degree of separation in buffer B 1
The citric acid of 2g amount is added, ultrasonic 5min, mixing in the buffer B 1 of 1000mL, through 0.45 μm of membrane filtration process, stand-by.By changing the amount of citric acid in buffer B 1, thus the pH value of buffer B 1 is changed to some extent, in sample component, the separation case of each material is as shown in the table, Gly-Glu under this condition in sample component and the degree of separation of alanine good, separation case simultaneously between other components is also fine, meets the requirement (as table 7) of test to sample separation degree.
Table 7: add the impact of citric acid on degree of separation in buffer B 1
。
Claims (3)
1. amino acid (15) two peptide (2) parenteral solution content assaying method, it is characterized in that: adopt ion exchange resin column, column temperature 52-62 DEG C, flow is 0.4ml/min, determined wavelength is 570nm and 440nm, mobile phase is by buffer B 1, B2, B3, B4, B5, B6 forms, elution time is 60 minutes, the B1 of 100% within 0-2.5 minute, is adopted to carry out wash-out, the B2 of 100% within 2.6-6 minute, is adopted to carry out wash-out, the B3 of 100% within 6.1-13.8 minute, is adopted to carry out wash-out, 13.9-29.0 the B4 of minute employing 100% carries out wash-out, 29.1-33 the B5 of minute employing 100% carries out wash-out, 33.1-34.0 the B2 of minute employing 100% carries out wash-out, 34.1-53 the B1 of minute employing 100% carries out wash-out,
Described buffer B 1 consists of 6.19g sodium citrate 2H
2o, 1MNaOH6-7mL, 5.66g sodium chloride, 19.80g citric acid H
2o, 135.0ml ethanol, 1-3g citric acid, be mixed with 1000mL solution with distilled water;
Described buffer B 2 consists of 7.74g sodium citrate 2H
2o, 1MNaOH20-22mL, 7.07g sodium chloride, 22.00g citric acid H
2o, 25.0ml ethanol, is mixed with 1000mL solution with distilled water;
Described buffer B 3 consists of 13.31g sodium citrate 2H
2o, 3.74g sodium chloride, 12.80g citric acid H
2o, 9.00ml ethanol, is mixed with 1000mL solution with distilled water;
Described buffer B 4 consists of 26.67g sodium citrate 2H
2o, 54.35g sodium chloride, 6.10g citric acid H
2o, is mixed with 1000mL solution with distilled water;
Described buffer B 5 consists of 8.00gNaOH, 100.0ml ethanol, is mixed with 1000mL solution with distilled water.
2. amino acid according to claim 1 (15) two peptide (2) parenteral solution content assaying method, is characterized in that: described column temperature is 57 DEG C.
3. amino acid according to claim 1 (15) two peptide (2) parenteral solution content assaying method, is characterized in that described buffer B 1 consists of 6.19g sodium citrate 2H
2o, 1MNaOH6-7mL, 5.66g sodium chloride, 19.80g citric acid H
2o, 135.0ml ethanol, 2g citric acid, be mixed with 1000mL solution with distilled water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410279676.9A CN105319282B (en) | 2014-06-20 | 2014-06-20 | Content measurement method of compound amino acid (15) dipeptide (2) injection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410279676.9A CN105319282B (en) | 2014-06-20 | 2014-06-20 | Content measurement method of compound amino acid (15) dipeptide (2) injection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105319282A true CN105319282A (en) | 2016-02-10 |
| CN105319282B CN105319282B (en) | 2017-04-19 |
Family
ID=55247134
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410279676.9A Active CN105319282B (en) | 2014-06-20 | 2014-06-20 | Content measurement method of compound amino acid (15) dipeptide (2) injection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105319282B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108008060A (en) * | 2017-09-21 | 2018-05-08 | 中国农业科学院农业质量标准与检测技术研究所 | The assay method and reagent of hydroxyproline in a kind of feed |
| CN110907584A (en) * | 2019-12-06 | 2020-03-24 | 鹤山市东古调味食品有限公司 | Method for detecting alcohol degree of non-alcoholic liquid |
| CN113384523A (en) * | 2021-06-29 | 2021-09-14 | 四川科伦药业股份有限公司 | Production and preparation method of compound amino acid (15) dipeptide (2) injection |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62113062A (en) * | 1985-11-13 | 1987-05-23 | Hitachi Ltd | Amino acid analyzer |
| CN102749397A (en) * | 2012-07-19 | 2012-10-24 | 天津金耀集团有限公司 | Analysis method of compound amino acid and dipeptide injection |
| CN103134892A (en) * | 2011-11-30 | 2013-06-05 | 天津金耀集团有限公司 | Analytical method of compound amino acid (15) double peptide (2) injection |
-
2014
- 2014-06-20 CN CN201410279676.9A patent/CN105319282B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62113062A (en) * | 1985-11-13 | 1987-05-23 | Hitachi Ltd | Amino acid analyzer |
| CN103134892A (en) * | 2011-11-30 | 2013-06-05 | 天津金耀集团有限公司 | Analytical method of compound amino acid (15) double peptide (2) injection |
| CN102749397A (en) * | 2012-07-19 | 2012-10-24 | 天津金耀集团有限公司 | Analysis method of compound amino acid and dipeptide injection |
Non-Patent Citations (7)
| Title |
|---|
| YOU-SHIN SHIM 等: "Method Validation of 16 Types of Structural Amino Acids using an Automated Amino Acid Analyzer", 《FOOD SCI. BIOTECHNOL.》 * |
| YUN DENG 等: "Thermal behavior, microstructure and protein quality of squid fillets dried by far-infrared assisted heat pump drying", 《FOOD CONTROL》 * |
| 叶晓林 等: "复方氨基酸（15）双肽（2）注射液中两组分的含量测定", 《药物鉴定》 * |
| 张韵 等: "炒焦对山楂中氨基酸含量的影响分析", 《氨基酸和生物资源》 * |
| 纪银福 等: "饲料中氨基酸的测定-酸水解法测试研究", 《草食家畜》 * |
| 陆明 等: "高效液相色谱法测定复方氨基酸（15）双肽（2）注射液中2种杂质的含量测定", 《药物分析杂志》 * |
| 高翠红 等: "日立L-8900型氨基酸分析仪用缓冲液的研究", 《氨基酸和生物资源》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108008060A (en) * | 2017-09-21 | 2018-05-08 | 中国农业科学院农业质量标准与检测技术研究所 | The assay method and reagent of hydroxyproline in a kind of feed |
| CN108008060B (en) * | 2017-09-21 | 2020-07-28 | 中国农业科学院农业质量标准与检测技术研究所 | Method and reagent for determining hydroxyproline in feed |
| CN110907584A (en) * | 2019-12-06 | 2020-03-24 | 鹤山市东古调味食品有限公司 | Method for detecting alcohol degree of non-alcoholic liquid |
| CN113384523A (en) * | 2021-06-29 | 2021-09-14 | 四川科伦药业股份有限公司 | Production and preparation method of compound amino acid (15) dipeptide (2) injection |
| CN113384523B (en) * | 2021-06-29 | 2022-06-03 | 四川科伦药业股份有限公司 | Production and preparation method of compound amino acid (15) dipeptide (2) injection |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105319282B (en) | 2017-04-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rigas | Liquid chromatography—post-column derivatization for amino acid analysis: Strategies, instrumentation, and applications | |
| CN104678044B (en) | Method for detection of free amino acids in tea by reversed-phase high-performance liquid chromatography | |
| WO2019207871A1 (en) | Rapid detection method for vitamin d in oil/fat or biological sample | |
| CN102323341A (en) | Method for detecting 18 varieties of protein hydrolytic amino acids in milk powder through high-performance liquid chromatographic method | |
| CN107843672B (en) | Method for detecting amino acid in serum by high performance liquid chromatography-tandem mass spectrometry | |
| CN106442352A (en) | Total serum protein detection kit with strong anti-interference capability | |
| Shim et al. | Method validation of 16 types of structural amino acids using an automated amino acid analyzer | |
| CN109342638A (en) | A method for detecting the contents of carnitrile, quaternary ammonium salt and its impurities ammonium, potassium and calcium ions by utilizing cation exchange inhibition conductivity method | |
| CN105319282B (en) | Content measurement method of compound amino acid (15) dipeptide (2) injection | |
| CN108535386A (en) | The method for measuring 5 kinds of component dissolution rates in FUFANG LIXUEPING PIAN with ultra-performance liquid chromatography | |
| CN105588900B (en) | Amino Acid Compound Injection 18AA II content assaying methods | |
| CN105929044B (en) | A kind of method of hydroxyproline content in quick detection milk and milk products | |
| Huang et al. | Simultaneous determination of eight biogenic amines in the traditional Chinese condiment Pixian Douban using UHPLC–MS/MS | |
| CN106248855B (en) | One assay method for growing tobacco middle biogenic amine | |
| CN102645464A (en) | Method for quickly detecting amino acids in yellow rice wine by using electronic tongue system | |
| CN104569134A (en) | Method for accurately testing digestion efficiency of proteins in matrix | |
| CN107091894A (en) | The method of Liquid Chromatography-Tandem Mass Spectrometry detection methylmalonic acid, methyl citric acid and/or homocysteine | |
| CN107271588B (en) | Method for measuring B vitamins by ultra-high performance liquid chromatography | |
| CN108828108A (en) | The method of many animals derived component in product containing gelatin crude drug is detected simultaneously | |
| CN105223280A (en) | A kind of black-bone chicken increases the assay method of amino acid content in emulsion formulation | |
| CN104215721B (en) | The application of phthalic acid in amino acid analysis | |
| Ren et al. | Spectrophotometric method for determination of tryptophan in protein hydrolysates | |
| CN111595969B (en) | Method for detecting compound three-dimensional D-calcium pantothenate syrup B vitamins by HPLC | |
| CN103197022A (en) | Method for detecting amino acid contained in table vinegar | |
| CN109406684A (en) | A kind of detection method measuring impurity B in the Amino Acid Compound Injection containing tryptophan, C, D content |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |