CN105191953A - Apple tree wound protective agent and preparation method thereof - Google Patents

Abstract

The invention provides an apple tree wound protective agent and a preparation method thereof. The apple tree wound protective agent comprises the following components by weight: 10 kg of vaseline, 10 kg of liquid paraffin, 2 kg of potassium humate, 3 kg of polyvinyl alcohol, 5 kg of glycerine, 0.0003 kg of IBA, 0.0002 kg of NAA, 0.0001 kg of 6-BA, 0.0001 kg of TDZ, 0.01 kg of Ca(NO3)2, 0.001 kg of FeSO4.7H2O and 70 kg of distilled water; the preparation method comprises the steps of dissolving potassium humate in water, dissolving liquid paraffin and compounding. The apple tree wound protective agent provided by the invention has the advantages of being highly efficient, safe, low-cost, low in toxicity, pollution-free and good in healing effect, and can rapidly form a film at or around wounds after being applied, so that the moisture is maintained, the cell division is stimulated, the cell viability is enhanced, the formation of callus tissues is promoted, germs infection is prevented, and the apple tree wound protective agent is suitable for being applied.

Description

A kind of apple tree wound protection agent and preparation method thereof

technical field

The invention proposes a chemical preparation used in the field of plant cultivation, specifically an apple tree wound protection agent and a preparation method thereof.

Background technique

Apple is the most important fruit tree in my country's economic status. Plastic pruning is an indispensable technical measure for apple tree building skeleton, adjusting light, promoting flowering and fruiting, and improving quality. If these wounds are not protected in time, they will be prone to frostbite, dryness, and death, resulting in obstruction of nutrient and water drainage, seriously weakening the tree vigor, affecting the healthy growth of the tree, and even causing rot Infection of pathogens causes dead trees to destroy gardens. According to Wang Caixia’s report in 2011, 80.04% of the new rot scars in spring came from saw cuts. Wu Yuxing’s investigation showed that saw cuts are the main site of ulcerative rot, with an average incidence of 47.4% and a maximum of 79.0%. Therefore, the rapid healing and healthy growth of the cuts are particularly important for the high quality, high yield and fruit safety of apples.

According to reports, my country used tung oil lead oil mixture, rosin clear oil mixture, soybean oil blue alum lime mixture, solid wax, lanolin, cow dung, yellow mud, etc. as wound protection agents in the 1980s and 1990s in China. Pollution and residues are serious, which cannot meet the production requirements of safety and non-toxicity. At present, most of the products on the market focus on sterilizing and inhibiting bacteria, and treating scars, and seldom consider the protection of healthy cuts, and the healing effect is not ideal. In recent years, some progress has been made in the research on growth regulators promoting wound healing. Zhang Xicai’s research shows that 6-BA and NAA have a certain effect on wound healing. Gao Yujun, Xu Guixuan’s research also proves that NAA is beneficial to wound healing. . In addition, with the development of science and technology, new high-end chemical products are used in agricultural production, providing new opportunities for the development of wound healing agents.

The existing apple tree wounds usually use paint and machine oil dressings, which have disadvantages such as poor healing effect, long residual time, polluting the environment, and inconvenient use.

Contents of the invention

In order to overcome the deficiencies in the prior art and materials, the invention proposes an apple tree wound protection agent and a preparation method thereof. The protective agent is composed of petrolatum, liquid paraffin, potassium humate, polyvinyl alcohol, glycerin, IBA, NAA, 6-BA, TDZ, Ca (NO ₃ ), FeSO ₄ 7H ₂ O and distilled water. Preparation Process. Solve the technical problem of apple tree wound protection.

The scheme that the present invention solves technical problem adopts is:

Apple tree wound protection agent consists of 10 kg of Vaseline, 10 kg of liquid paraffin, 2 kg of potassium humate, 3 kg of polyvinyl alcohol, 5 kg of glycerol, 0.0003 kg of IBA, 0.0002 kg of NAA, 0.0001 kg of 6-BA, TDZ 0.0001 kg, Ca (NO ₃ ) ₂ 0.01 kg, FeSO ₄ ·7H ₂ O 0.001 kg and distilled water 70 kg.

Preparation method of protective agent:

1. Dissolve potassium humate in distilled water, heat it in a water bath to 85°C, add polyvinyl alcohol and glycerin until completely dissolved; first dissolve IBA with a small amount of 1mol/L NaOH and then transfer to the above solution; NAA was dissolved with a small amount of 95% ethanol and then dissolved into the above solution; 6-BA and TDZ were completely dissolved with 0.1mol/L NaOH and then slowly added to the above solution; then Ca(NO ₃ ) ₂ and FeSO were added _{4.7H 2} O until dissolved, and prepared as solution A.

2. Heat the weighed vaseline and liquid paraffin in a water bath to 80°C, stir evenly to make a mixed solution B, and set aside.

3. Slowly add solution A to solution B, and stir with a mixer for 20 minutes, then cool naturally.

Positive effect, the apple tree wound protection agent of the present invention has the advantages of high efficiency, safety, cost saving, low toxicity, no pollution and good healing effect. Cell activity, promote callus formation, and prevent bacterial infection. It is suitable for application as apple tree wound protectant.

Detailed ways

Apple tree wound protection agent consists of 10 kg of Vaseline, 10 kg of liquid paraffin, 2 kg of potassium humate, 3 kg of polyvinyl alcohol, 5 kg of glycerol, 0.0003 kg of IBA, 0.0002 kg of NAA, 0.0001 kg of 6-BA, TDZ 0.0001 kg, Ca (NO ₃ ) ₂ 0.01 kg, FeSO ₄ ·7H ₂ O 0.001 kg and distilled water 70 kg.

Preparation method of protective agent:

1. Dissolve potassium humate in distilled water, heat it in a water bath to 85°C, add polyvinyl alcohol and glycerin until completely dissolved; first dissolve IBA with a small amount of 1mol/L NaOH and then transfer to the above solution; NAA was dissolved with a small amount of 95% ethanol and then dissolved into the above solution; 6-BA and TDZ were completely dissolved with 0.1mol/L NaOH and then slowly added to the above solution; then Ca(NO ₃ ) ₂ and FeSO were added _{4.7H 2} O until dissolved, and prepared as solution A.

2. Heat the weighed vaseline and liquid paraffin in a water bath to 80°C, stir evenly to make a mixed solution B, and set aside.

3. Slowly add solution A to solution B, and stir with a mixer for 20 minutes, then cool naturally.

Technical characteristics of the present invention:

1. For the first time, IBA, NAA, 6-BA and TDZ were used as healing factors to stimulate the production of callus and obtain the optimal formula ratio. 6-BA and TDZ are cytokinin growth regulators with high activity, which can stimulate the cells around the cut or wound to divide and quickly form callus. IBA and NAA are substances that promote growth, and cooperate with cytokinins to promote wound healing.

2. For the first time, polyvinyl alcohol was used as a film-forming agent to prepare a wound protectant. It is an organic compound with film-forming and hygroscopic properties, low toxicity, and low residue. It can quickly form a wound on the wound after application. The thin film has good moisturizing and breathable effects, and can promote the absorption of active ingredients by the phloem.

3. Potassium humate is a high-efficiency organic potassium fertilizer. After adding Ca ²⁺ and Fe ²⁺ , it can not only provide nutrients for wound healing, promote the rapid growth of callus, but also improve the stress resistance of fruit trees and enhance the resistance of wounds. frozen.

4. The glycerol and vaseline contained in the present invention have good water-locking and moisturizing functions. When applied to the wound, they can keep water for a long time, prevent water from evaporating, facilitate the absorption of active ingredients, and prevent xylem from drying out.

5. For the first time, Vaseline and liquid paraffin are mixed as the carrier of the main drug, and the main drug is completely stirred to form a solid oil emulsion, which is convenient for packaging and carrying. When using it, it can be directly applied to the wound with gloves or a small brush, which is convenient and saves time. work.

6. The ingredients used in the present invention are safe, non-toxic, green, and pollution-free. Vaseline, polyvinyl alcohol and other ingredients are widely used in medicine, food and cosmetics. Growth regulators such as NAA and 6-BA, potassium humate, etc. It is also widely used in agricultural production.

Field application test steps and results:

1. Test material: This test was carried out in the Experimental Demonstration Garden of the Institute of Pomology, Chinese Academy of Agricultural Sciences. The main plant variety Fuji was used as the test material. The field verification test was carried out for two consecutive years in 2012 and 2013. The tree body was 8 years old. The shape is elongated and spindle-shaped, the fertilizer and water management is at a medium level, and other management is normal.

2. Test method:

(1) Start treatment at the end of March every year. Select a saw cut with a diameter of 3-6cm in the middle and lower part of the tree body to apply the drug. Apply the drug immediately after cutting. Wear rubber gloves when applying, and apply to the wound. A layer of ointment with a thickness of 1-2mm should be applied evenly and in place to completely seal the wound.

Two treatments were set up in this experiment: treatment Ⅰ was the product, treatment Ⅱ was Guoshukang healing agent, and the wound without any protective agent was CK. There were 15 wounds in each treatment, 45 wounds in total.

(2) Survey method: Refer to Zhang Xicai's method with slight improvements. The investigation of wound healing was carried out after the leaves were completely defoliated in December every year. During the investigation, first cover the wound with white paper, draw the circumference of the wound and the circumference of the unhealed part, then take it back to the laboratory, and then use carbon paper to print on a slightly thicker cardboard, and cut out according to the circumference of the wound , the total area of the wound was obtained by scanning with a leaf area meter, and then cut along the circumference of the unhealed part to measure the unhealed area.

3. Test results and analysis

Through two consecutive years of field trials, good results have been achieved. This product can significantly improve the healing rate of the saw cut (P≤0.01), which was 40.93% and 32.38% higher than the control in 2012 and 2013, respectively. Compared with the products on the market, the healing rate of this product was 13.54% higher than that of Guoshukang in 2012, reaching a difference of 5%; in 2013, it was 15.42% higher, reaching a difference of 1%. It shows that the product developed by the present invention can significantly promote wound healing, and can be applied to apple production.

Claims (2)

1. an apple tree wound protection agent and preparation method thereof, is characterized in that: by 10 kilograms of petrolatum, 10 kilograms of liquid paraffin, 2 kilograms of potassium humate, 3 kilograms of polyvinyl alcohol, 5 kilograms of glycerol, 0.0003 kilograms of IBA , NAA 0.0002 kg, 6-BA 0.0001 kg, TDZ 0.0001 kg, Ca(NO ₃ ) ₂ 0.01 kg, FeSO ₄ ·7H ₂ O 0.001 kg and distilled water 70 kg. 2. a kind of apple tree wound protective agent according to claim 1 and preparation method thereof, is characterized in that: protective agent preparation method: 1) Dissolve potassium humate in distilled water, heat it in a water bath to 85°C, add polyvinyl alcohol and glycerin until completely dissolved; first dissolve IBA with a small amount of 1mol/L NaOH and then transfer to the above solution; NAA was dissolved with a small amount of 95% ethanol and then dissolved into the above solution; 6-BA and TDZ were completely dissolved with 0.1mol/L NaOH and then slowly added to the above solution; then Ca(NO ₃ ) ₂ and FeSO were added _{4.7H 2} O to dissolve and prepare into solution A; 2) Heat the weighed vaseline and liquid paraffin in a water bath to 80°C, stir evenly to make a mixed solution B, and set aside; 3) Slowly add solution A to solution B, and stir with a mixer for 20 minutes, then cool naturally.