CN104894856A - Processing method for improving mechanical properties of silk fibroin nanofiber - Google Patents
Processing method for improving mechanical properties of silk fibroin nanofiber Download PDFInfo
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- CN104894856A CN104894856A CN201510256456.9A CN201510256456A CN104894856A CN 104894856 A CN104894856 A CN 104894856A CN 201510256456 A CN201510256456 A CN 201510256456A CN 104894856 A CN104894856 A CN 104894856A
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Abstract
The invention relates to a processing method for improving mechanical properties of a silk fibroin nanofiber. The processing method comprises: dissolving EDC and NHS in a solvent to obtain an EDC/NHS solution; adding hyaluronic acid into the EDC/NHS solution for dissolving to obtain a solution; adding the silk fibroin nanofiber into the solution obtained from step (2), performing immersing, and performing vacuum drying. The processing method in the invention is simple and is relatively mild in reaction condition. The nanofiber obtained via cross-linking is not only excellent in biocompatibility, but also is relatively high in mechanical properties. The obtained nanofiber can be used as a support for tissue engineering or a patch for transdermal drug delivery, and has a bright application prospect in the field of biomedicine.
Description
Technical field
The invention belongs to the preparation field of nano fiber scaffold, particularly a kind of processing method promoting silk fibroin nano-fiber mechanical property.
Background technology
Fibroin albumen is a kind of native protein, has the advantages such as good biocompatibility, degradability and reduced immunogenicity.Silk fibroin nano-fiber is subject to extensive concern in the field of biomedical research such as medicine controlled releasing, organizational project.Silk fibroin nano-fiber can be prepared easily by electrostatic spinning technique.Electrospinning gained silk fibroin nano-fiber is water miscible, and application is gone to toward needing make its conformation change by certain post processing thus become water insoluble.The post-processing approach of conventional silk fibroin nano-fiber soaks or its steam treatment with the organic solvent such as methyl alcohol or ethanol.After such process, the mechanical property of silk fibroin nano-fiber is very poor, frangible, makes it apply and is subject to serious restriction.Therefore, find the aftertreatment technology that significantly can promote silk fibroin nano-fiber mechanical property to substitute current methods and become problem demanding prompt solution.
EDC (1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride) and NHS (N-hydroxy-succinamide) is the activating reagent being used as carboxyl in acid amides synthesis, also for being cross-linked of activated phosphate group, protein and nucleic acid and producing of immune conjugate.This crosslinking agent has good cell compatibility, can not have a negative impact while raising fibroin nanofiber film properties to the growth of cell.Hyaluronic acid (HA) is also a natural polysaccharide, has the advantages such as good biocompatibility, degradability and reduced immunogenicity equally, hyaluronicly adds the pliability that can strengthen silk fibroin nano-fiber film.
In sum, adopt aftertreatment technology as herein described, silk fibroin nano-fiber support not only mechanical property is significantly improved, and still possesses excellent biocompatibility, has application prospect at the biomedical sector such as regeneration, medicine controlled releasing.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of processing method promoting silk fibroin nano-fiber mechanical property, and the inventive method is simple, reaction condition is gentle, environmental protection, can be used for suitability for industrialized production; Products therefrom not only mechanical property significantly promotes, and possesses excellent biocompatibility, will greatly expand the application of silk fibroin nano-fiber at the biomedical sector such as regeneration, medicine controlled releasing.
A kind of processing method promoting silk fibroin nano-fiber mechanical property of the present invention, comprising:
(1) EDC and NHS is dissolved in a solvent, obtain EDC/NHS solution;
(2) hyaluronic acid is added above-mentioned EDC/NHS solution, dissolve, obtain solution;
(3) silk fibroin nano-fiber is placed in step (2) and obtains solution, soak, vacuumize.
In described step (1), solvent is ethanolic solution.
Described ethanolic solution to be concentration expressed in percentage by volume be 95% ethanol water.
In described step (2), dissolution time is 1-2h.
In the middle solution of described step (2), the concentration of EDC is the concentration of 150mM, NHS is 150mM, and hyaluronic concentration is 5-30mM.
In described step (3), the mass volume ratio of silk fibroin nano-fiber and solution is 1g/50ml.
In described step (3), soak time is 12-48h.
Described step (3) vacuumize temperature is 20-37 DEG C, and the time is 12-48h.
The concrete preparation method of silk fibroin nano-fiber is as follows:
1. fibroin albumen/PEO aqueous solution (mass volume ratio of fibroin albumen is 20%, PEO is 10% relative to the quality of fibroin albumen) is prepared.
2. 1. will carry out spinning by green electrospinning, voltage is 10kV, and fltting speed is 1.0mL/h, and receiving range is 15cm, and temperature is 25 DEG C, and humidity is 26.8%, prepares silk fibroin nano-fiber support.
beneficial effect
(1) simple to operate, the environmental protection of the present invention, can be used for suitability for industrialized production;
(2) the present invention can obtain silk fibroin nano-fiber support that mechanical property significantly improves and remain the excellent biocompatibility of natural material, has application prospect at biomedical sector.
Accompanying drawing explanation
Fig. 1 is that undressed electrospinning silk fibroin nano-fiber SEM schemes;
Fig. 2 is the SEM figure after the alcohol vapour crosslinking Treatment of electrospinning silk fibroin nano-fiber 75%;
Fig. 3 is the SEM figure of electrospinning silk fibroin nano-fiber after EDC-NHS/HA process in embodiment 1;
Fig. 4 is electrospinning silk fibroin nano-fiber load-deformation curve before and after EDC-NHS/HA (embodiment 1) or conventional alcohol vapour method process;
Fig. 5 is the SEM figure of electrospinning silk fibroin nano-fiber after EDC-NHS/HA process in embodiment 2;
Fig. 6 is electrospinning silk fibroin nano-fiber load-deformation curve before and after EDC-NHS/HA (embodiment 2) or conventional alcohol vapour method process.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Embodiment 1
(1) EDC and NHS is dissolved in ethanol water (concentration of volume percent is 95%), obtains EDC/NHS solution;
(2) hyaluronic acid is added above-mentioned EDC/NHS solution, dissolve 2h, obtain solution; Wherein in solution, the concentration of EDC is the concentration of 150mM, NHS is 150mM, and hyaluronic concentration is 5mM;
(3) silk fibroin nano-fiber is placed in step (2) and obtains solution, soak 12h, vacuumize 12h.
Embodiment 2
(1) EDC and NHS is dissolved in ethanol water (concentration of volume percent is 95%), obtains EDC/NHS solution;
(2) hyaluronic acid is added above-mentioned EDC/NHS solution, dissolve 2h, obtain solution; Wherein in solution, the concentration of EDC is the concentration of 150mM, NHS is 150mM, and hyaluronic concentration is 30mM;
(3) silk fibroin nano-fiber is placed in step (2) and obtains solution, soak 48h, vacuumize 12h.
Claims (8)
1. promote a processing method for silk fibroin nano-fiber mechanical property, comprising:
(1) EDC and NHS is dissolved in a solvent, obtain EDC/NHS solution;
(2) hyaluronic acid is added above-mentioned EDC/NHS solution, dissolve, obtain solution;
(3) silk fibroin nano-fiber is placed in step (2) and obtains solution, soak, vacuumize.
2. a kind of processing method promoting silk fibroin nano-fiber mechanical property according to claim 1, is characterized in that: in described step (1), solvent is ethanolic solution.
3. a kind of processing method promoting silk fibroin nano-fiber mechanical property according to claim 2, is characterized in that: described ethanolic solution to be concentration expressed in percentage by volume be 95% ethanol water.
4. a kind of processing method promoting silk fibroin nano-fiber mechanical property according to claim 1, is characterized in that: in described step (2), dissolution time is 1-2h.
5. a kind of processing method promoting silk fibroin nano-fiber mechanical property according to claim 1, it is characterized in that: in described step (2), in solution, the concentration of EDC is 150mM, the concentration of NHS is 150mM, and hyaluronic concentration is 5-30mM.
6. a kind of processing method promoting silk fibroin nano-fiber mechanical property according to claim 1, is characterized in that: in described step (3), the mass volume ratio of silk fibroin nano-fiber and solution is 1g:50ml.
7. a kind of processing method promoting silk fibroin nano-fiber mechanical property according to claim 1, is characterized in that: in described step (3), soak time is 12-48h.
8. a kind of processing method promoting silk fibroin nano-fiber mechanical property according to claim 1, is characterized in that: in described step (3), vacuumize temperature is 20-37 DEG C, and the time is 12-48h.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106176281A (en) * | 2016-07-26 | 2016-12-07 | 西安艾尔菲生物科技有限公司 | Carry the fibroin albumen/HA complex microsphere lyophilized powder of active component and preparation and application |
| CN107338648A (en) * | 2017-08-06 | 2017-11-10 | 长沙善道新材料科技有限公司 | A kind of preparation method of highly hygroscopic moisturizing stretch-proof silk fiber |
| CN107354748A (en) * | 2017-08-04 | 2017-11-17 | 浙江理工大学 | A kind of reinforcement means of fragile silk fabric cultural relics |
| CN109082887A (en) * | 2018-06-21 | 2018-12-25 | 湖北健客日化有限公司 | A kind of fabric softening agent |
| EP3369411A4 (en) * | 2015-04-28 | 2019-08-21 | Martin'ex International Research and Development | Multicomponent meso thread containing hyaluronic acid and method for producing same (variants) |
| CN110592936A (en) * | 2019-09-19 | 2019-12-20 | 西南大学 | Preparation of anti-ultraviolet silk fabric by using EDC/NHS solution |
| WO2021093110A1 (en) * | 2019-11-11 | 2021-05-20 | 山东安华生物医药股份有限公司 | Cross-linked hyaluronic acid dry powder and preparation method therefor |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3369411A4 (en) * | 2015-04-28 | 2019-08-21 | Martin'ex International Research and Development | Multicomponent meso thread containing hyaluronic acid and method for producing same (variants) |
| CN106176281A (en) * | 2016-07-26 | 2016-12-07 | 西安艾尔菲生物科技有限公司 | Carry the fibroin albumen/HA complex microsphere lyophilized powder of active component and preparation and application |
| CN106176281B (en) * | 2016-07-26 | 2019-06-28 | 西安艾尔菲生物科技有限公司 | Fibroin albumen/HA complex microsphere freeze-dried powder of load active constituent and preparation and application |
| CN107354748A (en) * | 2017-08-04 | 2017-11-17 | 浙江理工大学 | A kind of reinforcement means of fragile silk fabric cultural relics |
| CN107338648A (en) * | 2017-08-06 | 2017-11-10 | 长沙善道新材料科技有限公司 | A kind of preparation method of highly hygroscopic moisturizing stretch-proof silk fiber |
| CN109082887A (en) * | 2018-06-21 | 2018-12-25 | 湖北健客日化有限公司 | A kind of fabric softening agent |
| CN109082887B (en) * | 2018-06-21 | 2020-11-27 | 湖北健客日化有限公司 | Fabric softener |
| CN110592936A (en) * | 2019-09-19 | 2019-12-20 | 西南大学 | Preparation of anti-ultraviolet silk fabric by using EDC/NHS solution |
| WO2021093110A1 (en) * | 2019-11-11 | 2021-05-20 | 山东安华生物医药股份有限公司 | Cross-linked hyaluronic acid dry powder and preparation method therefor |
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