BR112019007719A2 - ácidos nucleicos compreendendo forquilhas imperfeitas - Google Patents
ácidos nucleicos compreendendo forquilhas imperfeitasInfo
- Publication number
- BR112019007719A2 BR112019007719A2 BR112019007719A BR112019007719A BR112019007719A2 BR 112019007719 A2 BR112019007719 A2 BR 112019007719A2 BR 112019007719 A BR112019007719 A BR 112019007719A BR 112019007719 A BR112019007719 A BR 112019007719A BR 112019007719 A2 BR112019007719 A2 BR 112019007719A2
- Authority
- BR
- Brazil
- Prior art keywords
- sequence
- duplex
- bases
- watson
- rna
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 4
- 230000000295 complement effect Effects 0.000 abstract 4
- 108020004999 messenger RNA Proteins 0.000 abstract 4
- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 125000006850 spacer group Chemical group 0.000 abstract 2
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/50—Methods for regulating/modulating their activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/50—Methods for regulating/modulating their activity
- C12N2320/53—Methods for regulating/modulating their activity reducing unwanted side-effects
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/50—Biochemical production, i.e. in a transformed host cell
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Abstract
a presente invenção proporciona um rna compreendendo na direção 5' para 3' uma primeira sequência e uma segunda sequência, estando a primeira sequência espaçada da segunda sequência por uma sequência espaçadora, sendo a primeira e segunda sequências substancialmente complementares e formando um dúplex através do emparelhamento de bases de watson e crick, sendo a segunda sequência capaz de hibridizar com o mrna de um gene alvo em um organismo, estando o rna substancialmente na forma de uma forquilha, sendo a segunda sequência totalmente complementar ao referido mrna ao longo da região em que hibridiza, a primeira sequência compreendendo bases de nucleotídeos que não emparelham como bases de watson e crick com a base correspondente na segunda sequência do dúplex, em que o dúplex não compreende regiões de 3 ou mais bases desemparelhadas consecutivas ou um rna compreendendo na direção 5' para 3' uma primeira sequência e uma segunda sequência, estando a primeira sequência espaçada da segunda sequência por uma sequência espaçadora, sendo a primeira e segunda sequências substancialmente complementares e formando um dúplex através do emparelhamento de bases de watson e crick, sendo a primeira sequência capaz de hibridizar com o mrna de um gene alvo em um organismo, estando o rna substancialmente na forma de uma forquilha, sendo a primeira sequência totalmente complementar ao referido mrna ao longo da região em que hibridiza, a segunda sequência compreendendo bases de nucleotídeos que não emparelham como bases de watson e crick com as bases de nucleotídeos correspondentes na primeira sequência do dúplex, em que o dúplex não compreende regiões de 3 ou mais bases desemparelhadas consecutivas.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/EP2016/075087 WO2018072821A1 (en) | 2016-10-19 | 2016-10-19 | Nucleic acids comprising imperfect hairpins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112019007719A2 true BR112019007719A2 (pt) | 2019-07-09 |
Family
ID=57223655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112019007719A BR112019007719A2 (pt) | 2016-10-19 | 2016-10-19 | ácidos nucleicos compreendendo forquilhas imperfeitas |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20200181611A1 (pt) |
| EP (1) | EP3529358A1 (pt) |
| CN (1) | CN109844114A (pt) |
| BR (1) | BR112019007719A2 (pt) |
| CA (1) | CA3037208A1 (pt) |
| RU (1) | RU2019114169A (pt) |
| WO (1) | WO2018072821A1 (pt) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030180756A1 (en) * | 2002-03-21 | 2003-09-25 | Yang Shi | Compositions and methods for suppressing eukaryotic gene expression |
| US7892793B2 (en) * | 2002-11-04 | 2011-02-22 | University Of Massachusetts | Allele-specific RNA interference |
| EP3587580A1 (en) * | 2010-10-27 | 2020-01-01 | Devgen NV | Down-regulating gene expression in insect pests |
| RU2015101749A (ru) * | 2012-06-22 | 2016-08-10 | Зингента Партисипейшнс Аг | Биологический контроль жесткокрылых вредителей |
| EP3067424A1 (en) * | 2015-03-13 | 2016-09-14 | Dow AgroSciences LLC | Rna polymerase i1 nucleic acid molecules to control insect pests |
-
2016
- 2016-10-19 WO PCT/EP2016/075087 patent/WO2018072821A1/en unknown
- 2016-10-19 CA CA3037208A patent/CA3037208A1/en not_active Abandoned
- 2016-10-19 RU RU2019114169A patent/RU2019114169A/ru unknown
- 2016-10-19 BR BR112019007719A patent/BR112019007719A2/pt not_active Application Discontinuation
- 2016-10-19 EP EP16790295.6A patent/EP3529358A1/en not_active Withdrawn
- 2016-10-19 CN CN201680089793.6A patent/CN109844114A/zh active Pending
- 2016-10-19 US US16/342,302 patent/US20200181611A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20200181611A1 (en) | 2020-06-11 |
| CN109844114A (zh) | 2019-06-04 |
| WO2018072821A1 (en) | 2018-04-26 |
| RU2019114169A3 (pt) | 2020-11-23 |
| CA3037208A1 (en) | 2018-04-26 |
| RU2019114169A (ru) | 2020-11-23 |
| EP3529358A1 (en) | 2019-08-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements | ||
| B350 | Update of information on the portal [chapter 15.35 patent gazette] |