Naldi et al., 2017 - Google Patents
Towards automation in protein digestion: Development of a monolithic trypsin immobilized reactor for highly efficient on-line digestion and analysisNaldi et al., 2017
View PDF- Document ID
- 12575275029234880305
- Author
- Naldi M
- Černigoj U
- Štrancar A
- Bartolini M
- Publication year
- Publication venue
- Talanta
External Links
Snippet
Reducing experimental variability, limiting contamination and increasing automation are essential goals in the development of reliable analytical platforms for mass spectrometry (MS)-based proteomics. In this work novel trypsin-based monolithic immobilized enzyme …
- 108090000631 Trypsin 0 title abstract description 169
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Naldi et al. | Towards automation in protein digestion: Development of a monolithic trypsin immobilized reactor for highly efficient on-line digestion and analysis | |
Zhang et al. | Systems analysis of singly and multiply O-glycosylated peptides in the human serum glycoproteome via EThcD and HCD mass spectrometry | |
Tholey et al. | Top-down proteomics for the analysis of proteolytic events-Methods, applications and perspectives | |
Tan et al. | Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states | |
EP2250186B1 (en) | High pressure enzymatic digestion system for protein characterization | |
Schley et al. | Proteome analysis of Myxococcus x anthus by off-line two-dimensional chromatographic separation using monolithic poly-(styrene-divinylbenzene) columns combined with ion-trap tandem mass spectrometry | |
Anderson et al. | Analyses of histone proteoforms using front-end electron transfer dissociation-enabled Orbitrap instruments | |
CN1714145A (en) | High Sensitivity Quantification of Peptides by Mass Spectrometry | |
CN107402269B (en) | Integrated proteomics sample pretreatment platform based on SCX/SAX mixed filler and application thereof | |
Shen et al. | Isolation and isotope labeling of cysteine-and methionine-containing tryptic peptides: application to the study of cell surface proteolysis | |
IL152620A (en) | Affinity selected signature peptides for protein identification and quantification | |
Aponte et al. | Streamlining bottom-up protein identification based on selective ultraviolet photodissociation (UVPD) of chromophore-tagged histidine-and tyrosine-containing peptides | |
CN111766325A (en) | A kind of sample pretreatment method for multi-omics analysis and its application | |
Jiang et al. | Technologies and methods for sample pretreatment in efficient proteome and peptidome analysis | |
Mayne et al. | Fine tuning of proteomic technologies to improve biological findings: advancements in 2011–2013 | |
Tran et al. | Addressing trypsin bias in large scale (phospho) proteome analysis by size exclusion chromatography and secondary digestion of large post-trypsin peptides | |
AU2019264349B2 (en) | Qualitative analysis of proteins | |
Kim et al. | Integrated sample preparation methodology for proteomics: analysis of native proteins | |
Mirzaei et al. | Structure specific chromatographic selection in targeted proteomics | |
Forstater et al. | Data-rich process development of immobilized biocatalysts in flow | |
US20080124781A1 (en) | Enzymes with modified amino acids | |
EP1783496B1 (en) | Method of analyzing protein structural affinity relationship | |
Miyagi et al. | Deuterium Labeling of Isoaspartic and Isoglutamic Acids for Mass Spectrometry Analysis | |
JP2010148442A (en) | Method for concentrating glycopeptide having sulfated sugar chain and kit therefor | |
Gao et al. | Integrated strong cation exchange/capillary reversed-phase liquid chromatography/on-target digestion coupled with mass spectrometry for identification of intact human liver tissue proteins |