(webin) [puttevdb@rivm-biohn-l05p test_upload]$ java -jar webin-cli-7.2.1.jar -context reads -username $ENA_USER -password $ENA_PASSWORD -manifest manifest_badread_first.txt -inputdir /data/BioGrid/puttevdb/test_upload/example_readsorting -validate | tee badread_first.out
INFO : Your application version is 7.2.1
INFO : A new application version is available. Please download the latest version 7.3.0 from https://github.com/enasequence/webin-cli/releases
INFO : Please upgrade to Webin-CLI version 7.0.1 or later if you see the following error: Failed to initialise validator. Could not retrieve BioSample.
ERROR: Reads must contain only valid IUPAC codes, with no more than 50% of bases being non-AUTCG
INFO : Creating report file: /data/BioGrid/puttevdb/test_upload/./webin-cli.report
uk.ac.ebi.ena.readtools.v2.validator.ReadsValidationException: Reads must contain only valid IUPAC codes, with no more than 50% of bases being non-AUTCG
	at uk.ac.ebi.ena.readtools.v2.validator.InsdcReadsValidator.validate(InsdcReadsValidator.java:113)
	at uk.ac.ebi.ena.readtools.v2.validator.ValidatorWrapper.validateInsdc(ValidatorWrapper.java:212)
	at uk.ac.ebi.ena.readtools.v2.validator.ValidatorWrapper.validateMultipleFastq(ValidatorWrapper.java:127)
	at uk.ac.ebi.ena.readtools.v2.validator.ValidatorWrapper.run(ValidatorWrapper.java:78)
	at uk.ac.ebi.ena.readtools.validator.ReadsValidator.runValidatorWrapper(ReadsValidator.java:105)
	at uk.ac.ebi.ena.readtools.validator.ReadsValidator.validate(ReadsValidator.java:84)
	at uk.ac.ebi.ena.readtools.validator.ReadsValidator.validate(ReadsValidator.java:69)
	at uk.ac.ebi.ena.readtools.validator.ReadsValidator.validate(ReadsValidator.java:57)
	at uk.ac.ebi.ena.readtools.validator.ReadsValidator.validate(ReadsValidator.java:35)
	at uk.ac.ebi.ena.webin.cli.WebinCliExecutor.validateSubmission(WebinCliExecutor.java:138)
	at uk.ac.ebi.ena.webin.cli.WebinCli.validate(WebinCli.java:317)
	at uk.ac.ebi.ena.webin.cli.WebinCli.execute(WebinCli.java:286)
	at uk.ac.ebi.ena.webin.cli.WebinCli.__main(WebinCli.java:122)
	at uk.ac.ebi.ena.webin.cli.WebinCli.main(WebinCli.java:90)
	at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
	at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:62)
	at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43)
	at java.lang.reflect.Method.invoke(Method.java:498)
	at org.springframework.boot.loader.MainMethodRunner.run(MainMethodRunner.java:49)
	at org.springframework.boot.loader.Launcher.launch(Launcher.java:108)
	at org.springframework.boot.loader.Launcher.launch(Launcher.java:58)
	at org.springframework.boot.loader.JarLauncher.main(JarLauncher.java:65)
ERROR: Submission validation failed because of a user error. Manifest name : test_001. See reports for details : /data/BioGrid/puttevdb/test_upload/reads/test_001/validate. All information is available in the output directory : /data/BioGrid/puttevdb/test_upload

(webin) [puttevdb@rivm-biohn-l05p test_upload]$ java -jar webin-cli-7.2.1.jar -context reads -username $ENA_USER -password $ENA_PASSWORD -manifest manifest_badread_last.txt -inputdir /data/BioGrid/puttevdb/test_upload/example_readsorting -validate | tee badread_last.out
INFO : Your application version is 7.2.1
INFO : A new application version is available. Please download the latest version 7.3.0 from https://github.com/enasequence/webin-cli/releases
INFO : Please upgrade to Webin-CLI version 7.0.1 or later if you see the following error: Failed to initialise validator. Could not retrieve BioSample.
INFO : Submission(s) validated successfully.
INFO : Creating report file: /data/BioGrid/puttevdb/test_upload/./webin-cli.report