8000 src/HisMaker.hh:11:20: fatal error: TFrame.h: No such file or directory · Issue #30 · TheJacksonLaboratory/SVE · GitHub
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Babita004 opened this issue Sep 2, 2019 · 8 comments

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@Babita004
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Hello,

I tried following command to install SVE:

export ROOTSYS=/home/programs
export LD_LIBRARY_PATH=$LD_LIBRARY_PATH:$ROOTSYS/lib

git clone --recursive https://github.com/TheJacksonLaboratory/SVE.git
cd SVE
make

I have got following error:

g++ -std=c++11 -m64 -O3 -DCNVNATOR_VERSION="v0.3" -I/home/Programs/include -Isrc/samtools -c src/cnvnator.cpp -o src/obj/cnvnator.o
In file included from src/cnvnator.cpp:8:0:
src/HisMaker.hh:11:20: fatal error: TFrame.h: No such file or directory
#include <TFrame.h>
^
compilation terminated.
make[3]: *** [src/obj/cnvnator.o] Error 1
make[2]: *** [cnvnator-multi] Error 2
make[1]: *** [all] Error 2
make: *** [speedseq] Error 2

Can you suggest me what I am doing wrong.

Thanks in advance

@Babita004
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Author

After compilation, I have got following error:

gcc -g -Wall -O2 -o bcftools call1.o main.o ../kstring.o ../bgzf.o ../knetfile.o ../bedidx.o -L. -lbcf -lm -lz -lpthread
make[5]: Nothing to be done for `all'.
g++ -std=c++11 -m64 -O3 -DCNVNATOR_VERSION="v0.3" -o bin/cnvnator-multi src/obj/cnvnator.o src/obj/HisMaker.o src/obj/AliParser.o src/obj/Genotyper.o src/obj/Interval.o src/obj/Genome.o src/obj/TreeBuilder.o src/samtools/libbam.a -lz -pthread -L/home/samir/Programs/root/builddir/lib -lCore -lCint -lRIO -lNet -lHist -lGraf -lGraf3d -lGpad -lTree -lRint -lMatrix -lPhysics -lMathCore -lThread -lGui
/usr/bin/ld: cannot find -lCint
collect2: error: ld returned 1 exit status
make[3]: *** [cnvnator-multi] Error 1
make[2]: *** [cnvnator-multi] Error 2
make[1]: *** [all] Error 2
make: *** [speedseq] Error 2

Please help me

@lslochov
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lslochov commented Sep 3, 2019

Hi @Babita004 , there's no need to use "-lCint" to compile CNVnator, so you can safely remove that option from the Makefile. The TFrame error happens if CNVnator can't find your ROOT installation.

@Babita004
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Babita004 commented Sep 4, 2019 via email

@lslochov
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lslochov commented Sep 4, 2019

This is on line 5 of the Makefile under "src/speedseq/src/cnvnator/Makefile"

@Babita004
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Author

Hello Sir,
I have already post this error previously but here I want to given complete details for understanding the problem.
Firstly, I have given following command for root environment and then run make command.

export ROOTSYS=/home/Programs/root/build

export PATH=$PATH:$ROOTSYS/bin

export LD_LIBRARY_PATH=$LD_LIBRARY_PATH:$ROOTSYS/lib

After running, I have got following error;

make

  • Unzip svtoolkit_2.00.1736
  • Building in htslib
    checking for gcc... gcc
    checking whether the C compiler works... yes
    checking for C compiler default output file name... a.out
    checking for suffix of executables...
    checking whether we are cross compiling... no
    checking for suffix of object files... o
    checking whether we are using the GNU C compiler... yes
    checking whether gcc accepts -g... yes
    checking for gcc option to accept ISO C89... none needed
    checking for ranlib... ranlib
    checking for special C compiler options needed for large files... no
    checking for _FILE_OFFSET_BITS value needed for large files... no
    checking shared library type... plain .so
    checking how to run the C preprocessor... gcc -E
    checking for grep that handles long lines and -e... /usr/bin/grep
    checking for egrep... /usr/bin/grep -E
    checking for ANSI C header files... yes
    checking for sys/types.h... yes
    checking for sys/stat.h... yes
    checking for stdlib.h... yes
    checking for string.h... yes
    checking for memory.h... yes
    checking for strings.h... yes
    checking for inttypes.h... yes
    checking for stdint.h... yes
    checking for unistd.h... yes
    checking for stdlib.h... (cached) yes
    checking for unistd.h... (cached) yes
    checking for sys/param.h... yes
    checking for getpagesize... yes
    checking for working mmap... yes
    checking for gmtime_r... yes
    checking whether fdatasync is declared... yes
    checking for fdatasync... yes
    checking for library containing log... -lm
    checking for zlib.h... yes
    checking for inflate in -lz... yes
    checking for library containing connect... none required
    checking for bzlib.h... yes
    checking for BZ2_bzBuffToBuffCompress in -lbz2... yes
    checking for curl_easy_pause in -lcurl... no
    checking for curl_easy_init in -lcurl... no
    configure: WARNING: libcurl not enabled: library not found
    configure: WARNING: GCS support not enabled: requires libcurl support
    configure: WARNING: S3 support not enabled: requires libcurl support
    configure: creating ./config.status
    config.status: creating config.mk
    config.status: creating htslib.pc.tmp
    config.status: creating config.h
    config.status: config.h is unchanged
    make --no-print-directory -C /home/Programs/SVE/src/htslib
    make[1]: Nothing to be done for `all'.
    samtools-0.1.19/
    samtools-0.1.19/bam_lpileup.c
    samtools-0.1.19/bam2depth.c
    samtools-0.1.19/bam_rmdupse.c
    samtools-0.1.19/faidx.h
    samtools-0.1.19/bamtk.c
    samtools-0.1.19/Makefile.mingw
    samtools-0.1.19/razf.c
    samtools-0.1.19/bedcov.c
    samtools-0.1.19/khash.h
    samtools-0.1.19/bam2bcf.h
    samtools-0.1.19/samtools.1
    samtools-0.1.19/bam_cat.c
    samtools-0.1.19/kaln.h
    samtools-0.1.19/bam_rmdup.c
    samtools-0.1.19/cut_target.c
    samtools-0.1.19/ksort.h
    samtools-0.1.19/bam_plcmd.c
    samtools-0.1.19/sample.h
    samtools-0.1.19/sam.c
    samtools-0.1.19/INSTALL
    samtools-0.1.19/bam_reheader.c
    samtools-0.1.19/bam.c
    samtools-0.1.19/bam.h
    samtools-0.1.19/errmod.h
    samtools-0.1.19/bedidx.c
    samtools-0.1.19/sample.c
    samtools-0.1.19/bam_aux.c
    samtools-0.1.19/Makefile
    samtools-0.1.19/COPYING
    samtools-0.1.19/bam_tview.c
    samtools-0.1.19/knetfile.c
    samtools-0.1.19/bgzf.c
    samtools-0.1.19/faidx.c
    samtools-0.1.19/sam_header.c
    samtools-0.1.19/bcftools/
    samtools-0.1.19/bcftools/bcf.h~
    samtools-0.1.19/bcftools/em.c
    samtools-0.1.19/bcftools/bcf.h
    samtools-0.1.19/bcftools/kmin.h
    samtools-0.1.19/bcftools/main.c
    samtools-0.1.19/bcftools/vcfutils.pl
    samtools-0.1.19/bcftools/fet.c
    samtools-0.1.19/bcftools/prob1.h
    samtools-0.1.19/bcftools/index.c
    samtools-0.1.19/bcftools/bcf2qcall.c
    samtools-0.1.19/bcftools/Makefile
    samtools-0.1.19/bcftools/bcfutils.c
    samtools-0.1.19/bcftools/README
    samtools-0.1.19/bcftools/prob1.c
    samtools-0.1.19/bcftools/vcf.c
    samtools-0.1.19/bcftools/mut.c
    samtools-0.1.19/bcftools/bcf.tex
    samtools-0.1.19/bcftools/bcf.c
    samtools-0.1.19/bcftools/kfunc.c
    samtools-0.1.19/bcftools/kmin.c
    samtools-0.1.19/bcftools/call1.c
    samtools-0.1.19/examples/
    samtools-0.1.19/examples/ex1.fa
    samtools-0.1.19/examples/chk_indel.c
    samtools-0.1.19/examples/Makefile
    samtools-0.1.19/examples/ex1.sam.gz
    samtools-0.1.19/examples/toy.sam
    samtools-0.1.19/examples/toy.fa
    samtools-0.1.19/examples/00README.txt
    samtools-0.1.19/examples/bam2bed.c
    samtools-0.1.19/examples/calDepth.c
    samtools-0.1.19/win32/
    samtools-0.1.19/win32/zlib.h
    samtools-0.1.19/win32/libcurses.a
    samtools-0.1.19/win32/libz.a
    samtools-0.1.19/win32/xcurses.h
    samtools-0.1.19/win32/zconf.h
    samtools-0.1.19/bam2bcf_indel.c
    samtools-0.1.19/kstring.c
    samtools-0.1.19/kstring.h
    samtools-0.1.19/kaln.c
    samtools-0.1.19/kseq.h
    samtools-0.1.19/bgzip.c
    samtools-0.1.19/bam2bcf.c
    samtools-0.1.19/bam_sort.c
    samtools-0.1.19/sam_view.c
    samtools-0.1.19/razf.h
    samtools-0.1.19/.gitignore
    samtools-0.1.19/knetfile.h
    samtools-0.1.19/bam_color.c
    samtools-0.1.19/bam_tview_html.c
    samtools-0.1.19/bam_import.c
    samtools-0.1.19/ChangeLog.old
    samtools-0.1.19/bam_endian.h
    samtools-0.1.19/kprobaln.c
    samtools-0.1.19/kprobaln.h
    samtools-0.1.19/razip.c
    samtools-0.1.19/misc/
    samtools-0.1.19/misc/wgsim.c
    samtools-0.1.19/misc/bowtie2sam.pl
    samtools-0.1.19/misc/r2plot.lua
    samtools-0.1.19/misc/vcfutils.lua
    samtools-0.1.19/misc/novo2sam.pl
    samtools-0.1.19/misc/samtools.pl
    samtools-0.1.19/misc/md5.h
    samtools-0.1.19/misc/psl2sam.pl
    samtools-0.1.19/misc/varfilter.py
    samtools-0.1.19/misc/Makefile
    samtools-0.1.19/misc/maq2sam.c
    samtools-0.1.19/misc/md5fa.c
    samtools-0.1.19/misc/export2sam.pl
    samtools-0.1.19/misc/sam2vcf.pl
    samtools-0.1.19/misc/soap2sam.pl
    samtools-0.1.19/misc/HmmGlocal.java
    samtools-0.1.19/misc/plot-bamcheck
    samtools-0.1.19/misc/bamcheck.c
    samtools-0.1.19/misc/md5.c
    samtools-0.1.19/misc/blast2sam.pl
    samtools-0.1.19/misc/interpolate_sam.pl
    samtools-0.1.19/misc/zoom2sam.pl
    samtools-0.1.19/misc/ace2sam.c
    samtools-0.1.19/misc/wgsim_eval.pl
    samtools-0.1.19/bam_mate.c
    samtools-0.1.19/bam_pileup.c
    samtools-0.1.19/bam_stat.c
    samtools-0.1.19/bam_md.c
    samtools-0.1.19/NEWS
    samtools-0.1.19/bam_tview_curses.c
    samtools-0.1.19/bamshuf.c
    samtools-0.1.19/bam.h~
    samtools-0.1.19/phase.c
    samtools-0.1.19/sam.h
    samtools-0.1.19/padding.c
    samtools-0.1.19/errmod.c
    samtools-0.1.19/AUTHORS
    samtools-0.1.19/klist.h
    samtools-0.1.19/bam_index.c
    samtools-0.1.19/bam_tview.h
    samtools-0.1.19/sam_header.h
    samtools-0.1.19/bgzf.h
  • Building in bwa
    make[1]: Nothing to be done for `all'.
  • Building in samtools
    cd ../htslib && make htslib_static.mk
    sed -n '/^static_libs=/s/[^=]=/HTSLIB_static_LIBS = /p;/^static_ldflags=/s/[^=]=/HTSLIB_static_LDFLAGS = /p' htslib.pc.tmp > htslib_static.mk
    make[1]: Nothing to be done for `all'.
  • Building in samtools-0.1.19
    make[3]: Nothing to be done for lib'. make[3]: Nothing to be done for lib'.
    make[3]: Nothing to be done for lib'. gcc -g -Wall -O2 -o samtools bam_tview.o bam_plcmd.o sam_view.o bam_rmdup.o bam_rmdupse.o bam_mate.o bam_stat.o bam_color.o bamtk.o kaln.o bam2bcf.o bam2bcf_indel.o errmod.o sample.o cut_target.o phase.o bam2depth.o padding.o bedcov.o bamshuf.o bam_tview_curses.o bam_tview_html.o libbam.a -Lbcftools -lbcf -lcurses -lm -lz -lpthread gcc -g -Wall -O2 -o bcftools call1.o main.o ../kstring.o ../bgzf.o ../knetfile.o ../bedidx.o -L. -lbcf -lm -lz -lpthread make[2]: Nothing to be done for all'.
  • Building in speedseq
    sed -i 's#CXX = g++ $(ROOTFLAGS)#CXX = g++ -std=c++11 $(ROOTFLAGS)#g' src/speedseq/src/cnvnator/Makefile
    make --no-print-directory -C src/speedseq
    make align
    make -C src/bwa
    make[3]: Nothing to be done for all'. cp src/bwa/bwa bin cp src/sambamba bin make -C src/samblaster make[3]: Nothing to be done for SAMBLASTER'.
    cp src/samblaster/samblaster bin
    cd src/parallel; ./configure --prefix=/home/Programs/SVE/src/speedseq
    checking for a BSD-compatible install... /usr/bin/install -c
    checking whether build environment is sane... yes
    checking for a thread-safe mkdir -p... /usr/bin/mkdir -p
    checking for gawk... gawk
    checking whether make sets $(MAKE)... yes
    configure: creating ./config.status
    config.status: creating Makefile
    config.status: creating src/Makefile
    config.status: creating config.h
    config.status: config.h is unchanged
    make -C src/parallel
    make all-recursive
    Making all in src
    pod2man --release='20131022' --center='parallel'
    --section=1 ./parallel.pod > ./parallel.1
    pod2man --release='20131022' --center='parallel'
    --section=1 ./sem.pod > ./sem.1
    pod2man --release='20131022' --center='parallel'
    --section=1 ./sql > ./sql.1
    pod2man --release='20131022' --center='parallel'
    --section=1 ./niceload.pod > ./niceload.1
    pod2man --release='20131022' --center='parallel'
    --section=1 ./parallel_tutorial.pod > ./parallel_tutorial.1
    pod2html ./parallel.pod > ./parallel.html
    rm -f ./pod2htm*
    pod2html ./parallel_tutorial.pod > ./parallel_tutorial.html
    rm -f ./pod2htm*
    pod2html ./sem.pod > ./sem.html
    rm -f ./pod2htm*
    pod2html ./sql > ./sql.html
    rm -f ./pod2htm*
    pod2html ./niceload.pod > ./niceload.html
    rm -f ./pod2htm*
    pod2pdf --output-file ./parallel.pdf ./parallel.pod --title "GNU Parallel" || true
    /bin/sh: pod2pdf: command not found
    pod2pdf --output-file ./parallel_tutorial.pdf ./parallel_tutorial.pod --title "GNU Parallel Tutorial" || true
    /bin/sh: pod2pdf: command not found
    cp src/parallel/src/parallel bin
    make var
    make -C src/freebayes
    wget -q http://hypervolu.me/freebayes/build/v0.9.21 &
    cd src && make
    DETECTED_VERSION = v0.9.21
    CURRENT_VERSION = v0.9.21
    cp src/freebayes/bin/freebayes bin
    make -C src/tabix
    gcc -g -Wall -O2 -fPIC -o tabix main.o -L. -ltabix -lm -lz
    cp src/tabix/tabix bin
    cp src/tabix/bgzip bin
    cp src/vawk/vawk bin
    cd src/parallel; ./configure --prefix=/home/Programs/SVE/src/speedseq
    checking for a BSD-compatible install... /usr/bin/install -c
    checking whether build environment is sane... yes
    checking for a thread-safe mkdir -p... /usr/bin/mkdir -p
    checking for gawk... gawk
    checking whether make sets $(MAKE)... yes
    configure: creating ./config.status
    config.status: creating Makefile
    config.status: creating src/Makefile
    config.status: creating config.h
    config.status: config.h is unchanged
    make -C src/parallel
    make all-recursive
    Making all in src
    pod2man --release='20131022' --center='parallel'
    --section=1 ./parallel.pod > ./parallel.1
    pod2man --release='20131022' --center='parallel'
    --section=1 ./sem.pod > ./sem.1
    pod2man --release='20131022' --center='parallel'
    --section=1 ./sql > ./sql.1
    pod2man --release='20131022' --center='parallel'
    --section=1 ./niceload.pod > ./niceload.1
    pod2man --release='20131022' --center='parallel'
    --section=1 ./parallel_tutorial.pod > ./parallel_tutorial.1
    pod2html ./parallel.pod > ./parallel.html
    rm -f ./pod2htm*
    pod2html ./parallel_tutorial.pod > ./parallel_tutorial.html
    rm -f ./pod2htm*
    pod2html ./sem.pod > ./sem.html
    rm -f ./pod2htm*
    pod2html ./sql > ./sql.html
    rm -f ./pod2htm*
    pod2html ./niceload.pod > ./niceload.html
    rm -f ./pod2htm*
    pod2pdf --output-file ./parallel.pdf ./parallel.pod --title "GNU Parallel" || true
    /bin/sh: pod2pdf: command not found
    pod2pdf --output-file ./parallel_tutorial.pdf ./parallel_tutorial.pod --title "GNU Parallel Tutorial" || true
    /bin/sh: pod2pdf: command not found
    cp src/parallel/src/parallel bin
    make sv
    make -C src/lumpy-sv
    [ -d bin ] || mkdir -p bin
    cp scripts/lumpyexpress bin/lumpyexpress

bin/lumpyexpress.config
which: no samblaster in (/home/Programs/root/build/bin:/home/Programs/root/bin:/root/anaconda2/bin:/root/anaconda3/bin:/root/anaconda2/bin:/root/anaconda3/bin:/home/perl5/perlbrew/bin:/usr/local/bin:/usr/local/sbin:/usr/bin:/usr/sbin:/bin:/sbin:/home/.local/bin:/home/bin:/home/Programs/root/builddir/bin:/home/Programs/root/builddir/bin:/home/Programs/root/builddir/bin)
which: no sambamba in (/home/Programs/root/build/bin:/home/Programs/root/bin:/root/anaconda2/bin:/root/anaconda3/bin:/root/anaconda2/bin:/root/anaconda3/bin:/home/perl5/perlbrew/bin:/usr/local/bin:/usr/local/sbin:/usr/bin:/usr/sbin:/bin:/sbin:/home/.local/bin:/home/bin:/home/Programs/root/builddir/bin:/home/Programs/root/builddi 8000 r/bin:/home/Programs/root/builddir/bin)
[ -d obj ] || mkdir -p obj
[ -d bin ] || mkdir -p bin
Building lumpy:
=========================================================

  • Creating BamTools API
  • Building in src/utils/bedFile
    make[4]: `../../../obj//bedFile.o' is up to date.

  • Building in src/utils/version
    make[4]: Nothing to be done for `all'.

  • Building in src/utils/gzstream
    make[4]: `../../../obj//gzstream.o' is up to date.

  • Building in src/utils/fileType
    make[4]: `../../../obj//fileType.o' is up to date.

  • Building in src/utils/bedFilePE
    make[4]: `../../../obj//bedFilePE.o' is up to date.

  • Building in src/utils/genomeFile
    make[4]: `../../../obj//genomeFile.o' is up to date.

  • Building in src/utils/BamTools
    make[4]: Nothing to be done for `all'.

  • Building in src/utils/BamTools-Ancillary
    make[4]: Nothing to be done for `all'.

  • Building in src/utils/sequenceUtilities
    make[4]: `../../../obj//sequenceUtils.o' is up to date.

  • Building in src/utils/sqlite3
    make[4]: `../../../obj//sqlite3.o' is up to date.

  • Building in src/lumpy

    • linking lumpy

cp src/lumpy-sv/scripts/pairend_distro.py bin
cp src/lumpy-sv/bin/lumpy bin
cp src/lumpy-sv/bin/lumpyexpress bin
cp src/lumpy-sv/scripts/vcfToBedpe bin
cp src/sambamba bin
make -C src/samblaster
make[3]: Nothing to be done for SAMBLASTER'. cp src/samblaster/samblaster bin cp src/vawk/vawk bin cp src/bamkit/bamtofastq.py bin cp src/bamkit/bamheadrg.py bin cp src/bamkit/bamgroupreads.py bin cp src/bamkit/bamfilterrg.py bin cp src/bamkit/bamcleanheader.py bin cp src/bamkit/bamlibs.py bin make -C src/tabix gcc -g -Wall -O2 -fPIC -o tabix main.o -L. -ltabix -lm -lz cp src/tabix/tabix bin cp src/tabix/bgzip bin cp src/svtyper/svtyper bin make -C src/cnvnator make -C src/samtools make[6]: Nothing to be done for lib'.
make[6]: Nothing to be done for lib'. make[6]: Nothing to be done for lib'.
gcc -g -Wall -O2 -o samtools bam_tview.o bam_plcmd.o sam_view.o bam_rmdup.o bam_rmdupse.o bam_mate.o bam_stat.o bam_color.o bamtk.o kaln.o bam2bcf.o bam2bcf_indel.o errmod.o sample.o cut_target.o phase.o bam2depth.o padding.o bedcov.o bamshuf.o bam_tview_curses.o bam_tview_html.o libbam.a -Lbcftools -lbcf -lcurses -lm -lz -lpthread
gcc -g -Wall -O2 -o bcftools call1.o main.o ../kstring.o ../bgzf.o ../knetfile.o ../bedidx.o -L. -lbcf -lm -lz -lpthread
make[5]: Nothing to be done for `all'.
g++ -std=c++11 -m64 -O3 -DCNVNATOR_VERSION="v0.3" -o bin/cnvnator-multi src/obj/cnvnator.o src/obj/HisMaker.o src/obj/AliParser.o src/obj/Genotyper.o src/obj/Interval.o src/obj/Genome.o src/obj/TreeBuilder.o src/samtools/libbam.a -lz -pthread -L/home/Programs/root/build/lib -lCore -lCint -lRIO -lNet -lHist -lGraf -lGraf3d -lGpad -lTree -lRint -lMatrix -lPhysics -lMathCore -lThread -lGui
/usr/bin/ld: cannot find -lCint
collect2: error: ld returned 1 exit status
make[3]: *** [cnvnator-multi] Error 1
make[2]: *** [cnvnator-multi] Error 2
make[1]: *** [all] Error 2
make: *** [speedseq] Error 2

I have also removed removed "-lCint" options from src/speedseq/src/cnvnator/Makefile. Unfortunately, I have got similar error.

Please give me suggestions and help me. I don't know what I am doing wrong

Thanks in advance

@lslochov
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Hi, I think what's going on is that running "make" will attempt to install a fresh copy of CNVnator, so you also need to modify a line in the top level Makefile (the one in the SVE folder) so that it uses the existing copy.

On line 109, change this line:

@cd $(SVE_DIR)/$(SRC) && tar -zxvf CNVnator_v0.3.3.tar.gz

to:

@cd $(SVE_DIR)/$(SRC) # && tar -zxvf CNVnator_v0.3.3.tar.gz

Then go to the CNVnator Makefile under Speedseq and remove "-lCint" again. Then trying compiling the whole thing with "make"

@Babita004
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Author
Babita004 commented Sep 17, 2019

Hello lslochov
Thank you so much for your suggestions. Sorry for delay response.
I have make changes in the both Makefile and then run the again make command. After your suggestions, previous error is solved but unfortunately following error come again;

Score floating point type: double
-- Performing Test CXX11_FLAG0
-- Performing Test CXX11_FLAG0 - Success
-- C++11 support enabled via -std=c++11
-- Performing Test CXX11_STDLIB_FLAG0
-- Performing Test CXX11_STDLIB_FLAG0 - Success
-- Sufficient C++11 library support found with flag ' '
-- Configuring done
-- Generating done
-- Build files have been written to: /home/Programs/SVE/src/breakdancer-1.4.5
make[1]: Entering directory /home/Programs/SVE/src/breakdancer-1.4.5/build' make[1]: *** No targets specified and no makefile found. Stop. make[1]: Leaving directory /home/Programs/SVE/src/breakdancer-1.4.5/build'
make: *** [breakdancer] Error 2

Please help me and give the suggestions.
Thanks in advance

@Babita004
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Hello,

Please help me and give the suggestions. I don't know what I am doing wrong

Thank You

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