ENZYME entry: EC 2.5.1.3

Accepted Name

thiamine phosphate synthase

Alternative Name(s)

thiamine-phosphate diphosphorylase

thiamine-phosphate pyrophosphorylase

thiamine-phosphate synthase

TMP diphosphorylase

TMP pyrophosphorylase

Reaction catalysed

2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate
2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = thiamine phosphate + CO2 + diphosphate
4-methyl-5-(2-phosphooxyethyl)-thiazole + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + H(+) = thiamine phosphate + diphosphate

Comment(s)

The enzyme catalyzes the penultimate reaction in thiamine de novo biosynthesis, condensing the pyrimidine and thiazole components.
The enzyme is thought to accept the product of EC 2.8.1.10 as its substrate.
However, it has been shown that in some bacteria, such as Bacillus subtilis, an additional enzyme, EC 5.3.99.10 converts that compound into its tautomer 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate, and that it is the latter that serves as the substrate for the synthase.
In addition to this activity, the enzyme participates in a salvage pathway, acting on 4-methyl-5-(2-phosphono-oxyethyl)thiazole, which is produced from thiamine degradation products.
In yeast this activity is found in a bifunctional enzyme and in the plant Arabidopsis thaliana the activity is part of a trifunctional enzyme.

Cross-references

BRENDA

2.5.1.3

EC2PDB

2.5.1.3

ExplorEnz

2.5.1.3

PRIAM enzyme-specific profiles

2.5.1.3

KEGG Ligand Database for Enzyme Nomenclature

2.5.1.3

IUBMB Enzyme Nomenclature

2.5.1.3

IntEnz

2.5.1.3

MEDLINE

Find literature relating to 2.5.1.3

MetaCyc

2.5.1.3

Rhea expert-curated reactions

2.5.1.3

UniProtKB/Swiss-Prot

P40386, THI6_SCHPO	P41835, THI6_YEAST	Q57688, THIDN_METJA
Q9WZP7, THIDN_THEMA	O66833, THIE1_AQUAE	Q97RS5, THIE1_STRPN
Q8DQK4, THIE1_STRR6	O67378, THIE2_AQUAE	P66920, THIE2_STRPN
P66921, THIE2_STRR6	Q8G7X1, THIEC_BIFLO	Q8FTH8, THIED_COREF
Q8NQH1, THIED_CORGL	P61422, THIED_GEOSL	A0LTK3, THIE_ACIC1
B7JBI2, THIE_ACIF2	B5ERP0, THIE_ACIF5	B3GX82, THIE_ACTP7
A6VPG9, THIE_ACTSZ	Q8UAS8, THIE_AGRFC	Q21VL5, THIE_ALBFT
Q0VSP5, THIE_ALCBS	A6TMN5, THIE_ALKMQ	A8MK92, THIE_ALKOO
Q2IM25, THIE_ANADE	B7GKQ8, THIE_ANOFW	O28205, THIE_ARCFU
Q5P6J5, THIE_AROAE	A0JYP1, THIE_ARTS2	A8IGE6, THIE_AZOC5
A1KB60, THIE_AZOSB	C1DMY6, THIE_AZOVD	C3PBW1, THIE_BACAA
C3L627, THIE_BACAC	A0R981, THIE_BACAH	Q81Z95, THIE_BACAN
B7JN72, THIE_BACC0	P61410, THIE_BACC1	C1EVE6, THIE_BACC3
B7H7H5, THIE_BACC4	B7HT70, THIE_BACC7	A7GKR4, THIE_BACCN
B9J2L5, THIE_BACCQ	Q81IG8, THIE_BACCR	Q63GK3, THIE_BACCZ
Q5LCA7, THIE_BACFN	Q64TA1, THIE_BACFR	Q6HP17, THIE_BACHK
Q65DK0, THIE_BACLD	A9VRN4, THIE_BACMK	A8FIR6, THIE_BACP2
P39594, THIE_BACSU	Q8AA13, THIE_BACTN	A7ZA58, THIE_BACVZ
Q2KUS6, THIE_BORA1	Q7WF72, THIE_BORBR	Q7W3U2, THIE_BORPA
Q7W049, THIE_BORPE	B9MN52, THIE_CALBD	A8M9N4, THIE_CALMQ
Q8R806, THIE_CALS4	A4XG66, THIE_CALS8	A8FMD4, THIE_CAMJ8
Q9PNL3, THIE_CAMJE	A1W068, THIE_CAMJJ	Q5HU23, THIE_CAMJR
B0SUK3, THIE_CAUSK	A9WDL8, THIE_CHLAA	Q5L6R5, THIE_CHLAB
Q824E9, THIE_CHLCV	B3ED41, THIE_CHLL2	Q3B4B1, THIE_CHLL3
B3QNM2, THIE_CHLP8	A1BGM7, THIE_CHLPD	A4SEP8, THIE_CHLPM
Q8KD79, THIE_CHLTE	Q7P1R3, THIE_CHRVO	A8AKT0, THIE_CITK8
Q97LQ9, THIE_CLOAB	A7FPT0, THIE_CLOB1	C3L061, THIE_CLOB6
A6M1W6, THIE_CLOB8	B2UY65, THIE_CLOBA	B2TQ13, THIE_CLOBB
A5HYZ3, THIE_CLOBH	C1FSC1, THIE_CLOBJ	B1IEG6, THIE_CLOBK
A7GAL0, THIE_CLOBL	B1KV12, THIE_CLOBM	Q186F8, THIE_CLOD6
A0Q2A0, THIE_CLONN	Q0TQV4, THIE_CLOP1	Q8XKQ8, THIE_CLOPE
Q0STA1, THIE_CLOPS	Q893R0, THIE_CLOTE	B5Y6I1, THIE_COPPD
Q8FP55, THIE_COREF	Q4JVZ1, THIE_CORJK	A7MJ80, THIE_CROS8
Q478V2, THIE_DECAR	Q1IX16, THIE_DEIGD	Q9RYX9, THIE_DEIRA
B8FUD4, THIE_DESHD	Q24XQ0, THIE_DESHY	B5YA97, THIE_DICT6
B8E358, THIE_DICTD	A7ZUK8, THIE_ECO24	Q8X6Y0, THIE_ECO57
B5Z089, THIE_ECO5E	A8A793, THIE_ECOHS	A1AIG4, THIE_ECOK1
Q0TA72, THIE_ECOL5	Q8FB78, THIE_ECOL6	B1IUQ4, THIE_ECOLC
P30137, THIE_ECOLI	B6I5K4, THIE_ECOSE	Q1R5V9, THIE_ECOUT
A4W5B6, THIE_ENT38	Q830K5, THIE_ENTFA	Q2NB00, THIE_ERYLH
A5FJJ1, THIE_FLAJ1	A6GWR9, THIE_FLAPJ	Q8RI59, THIE_FUSNN
Q5KZU0, THIE_GEOKA	P61411, THIE_GEOSL	A4IN28, THIE_GEOTN
Q7NNK8, THIE_GLOVI	Q4QNC6, THIE_HAEI8	A5UA71, THIE_HAEIE
A5UGT0, THIE_HAEIG	P71350, THIE_HAEIN	Q9KCY8, THIE_HALH5
A1WYL4, THIE_HALHL	Q5V0G6, THIE_HALMA	Q18GX9, THIE_HALWD
Q1CT27, THIE_HELPH	Q9ZL01, THIE_HELPJ	B2USY5, THIE_HELPS
O25514, THIE_HELPY	A9AZD9, THIE_HERA2	Q0I131, THIE_HISS1
B4U6B2, THIE_HYDS0	Q0BXD3, THIE_HYPNA	A6SUR1, THIE_JANMA
A6W6A2, THIE_KINRD	A6TGQ0, THIE_KLEP7	Q1ILG3, THIE_KORVE
B3W783, THIE_LACCB	Q9CG48, THIE_LACLA	A2RKJ7, THIE_LACLM
Q02YS6, THIE_LACLS	Q03CB2, THIE_LACP3	Q890C0, THIE_LACPL
C1DCM3, THIE_LARHH	Q38ZM1, THIE_LATSS	Q1MRJ6, THIE_LAWIP
B0SE83, THIE_LEPBA	B0SMR9, THIE_LEPBP	B1MX63, THIE_LEUCK
Q03NS1, THIE_LEVBA	B2GBV5, THIE_LIMF3	A5VKA1, THIE_LIMRD
B2G7Q4, THIE_LIMRJ	Q92EW5, THIE_LISIN	C1KZ34, THIE_LISMC
Q723Y9, THIE_LISMF	B8DET0, THIE_LISMH	Q8YA44, THIE_LISMO
A0AFC5, THIE_LISW6	B1HX34, THIE_LYSSC	Q65US7, THIE_MANSM
A1TYG6, THIE_MARN8	Q8TMD6, THIE_METAC	Q0W1L8, THIE_METAR
A7IA09, THIE_METB6	Q466J5, THIE_METBF	Q602R3, THIE_METCA
Q1GXW1, THIE_METFK	Q2FM63, THIE_METHJ	A4FX32, THIE_METM5
A9AAG9, THIE_METM6	A6VG83, THIE_METM7	Q8PS49, THIE_METMA
A3CS45, THIE_METMJ	P61413, THIE_METMP	A5ULP4, THIE_METS3
A6UPE6, THIE_METVS	Q2RGI8, THIE_MOOTA	A0QLT6, THIE_MYCA1
P66917, THIE_MYCBO	A1KFN6, THIE_MYCBP	A4T2R5, THIE_MYCGI
B8ZU95, THIE_MYCLB	Q9ZBL5, THIE_MYCLE	B2HPZ9, THIE_MYCMM
P61412, THIE_MYCPA	A0QQK7, THIE_MYCS2	A3PTW9, THIE_MYCSJ
A1UAB4, THIE_MYCSK	Q1BEL9, THIE_MYCSS	A5TZE0, THIE_MYCTA
P9WG74, THIE_MYCTO	P9WG75, THIE_MYCTU	A0PRY2, THIE_MYCUA
A1T2Z5, THIE_MYCVP	Q1D282, THIE_MYXXD	Q3IP34, THIE_NATPD
Q5F5C2, THIE_NEIG1	Q9JWI2, THIE_NEIMA	Q9JXF7, THIE_NEIMB
Q0AFV0, THIE_NITEC	Q82UQ7, THIE_NITEU	Q2Y5Q6, THIE_NITMU
Q3JDH3, THIE_NITOC	Q5YNP9, THIE_NOCFA	B2J6F7, THIE_NOSP7
Q8YX72, THIE_NOSS1	Q8ESZ3, THIE_OCEIH	Q7U5U1, THIE_PARMW
P57930, THIE_PASMU	Q6DAM1, THIE_PECAS	Q03GM4, THIE_PEDPA
A5D4K4, THIE_PELTS	Q4FN35, THIE_PELUB	C0QR82, THIE_PERMH
Q7N964, THIE_PHOLL	A6L645, THIE_PHOV8	Q6KZH9, THIE_PICTO
A2CB21, THIE_PROM3	Q7VAV5, THIE_PROMA	B4EYU2, THIE_PROMH
Q7V8I3, THIE_PROMM	Q7V0I3, THIE_PROMP	A2BSJ5, THIE_PROMS
Q48DP2, THIE_PSE14	A6V0D3, THIE_PSEA7	B7V9E0, THIE_PSEA8
Q02SE4, THIE_PSEAB	Q9HX40, THIE_PSEAE	Q1I4H6, THIE_PSEE4
Q4K5I7, THIE_PSEF5	C3K2K2, THIE_PSEFS	A4XYV5, THIE_PSEMY
A5W9G9, THIE_PSEP1	Q3K6C1, THIE_PSEPF	B0KJV8, THIE_PSEPG
Q88DP1, THIE_PSEPK	B1J1Y7, THIE_PSEPW	Q87VY6, THIE_PSESM
Q4ZNA1, THIE_PSEU2	A5WDP0, THIE_PSYWF	Q9UZQ5, THIE_PYRAB
Q8U192, THIE_PYRFU	O58878, THIE_PYRHO	O34294, THIE_RHIEC
Q7UQH1, THIE_RHOBA	B6INN5, THIE_RHOCS	A7NRF7, THIE_ROSCS
A5UUL3, THIE_ROSS1	B8I3J4, THIE_RUMCH	Q21MI9, THIE_SACD2
A4F727, THIE_SACEN	B5F1H6, THIE_SALA4	A8M4E6, THIE_SALAI
A9MHD7, THIE_SALAR	Q57H62, THIE_SALCH	B5FQK8, THIE_SALDC
B5QYE8, THIE_SALEP	B5RFJ1, THIE_SALG2	B4TCT2, THIE_SALHS
B4T0Z5, THIE_SALNS	Q5PKA7, THIE_SALPA	A9N0K5, THIE_SALPB
B5BJR2, THIE_SALPK	Q2S2A8, THIE_SALRD	B4TQK3, THIE_SALSV
Q8Z325, THIE_SALTI	A4XBL2, THIE_SALTO	Q9L9I8, THIE_SALTY
A8G8F3, THIE_SERP5	B2TWI0, THIE_SHIB3	Q31U04, THIE_SHIBS
Q32AG6, THIE_SHIDS	Q0SY07, THIE_SHIF8	Q83PB9, THIE_SHIFL
Q3YUZ1, THIE_SHISS	Q5WH87, THIE_SHOC1	C4XIJ2, THIE_SOLM1
A7X4S8, THIE_STAA1	A6U3H7, THIE_STAA2	Q2FF35, THIE_STAA3
Q2FWG3, THIE_STAA8	A5IUN7, THIE_STAA9	Q2YUL2, THIE_STAAB
Q5HEA8, THIE_STAAC	A6QIT5, THIE_STAAE	P66918, THIE_STAAM
P66919, THIE_STAAN	Q6GEY4, THIE_STAAR	Q6G7L9, THIE_STAAS
Q8NVH5, THIE_STAAW	Q9RGS5, THIE_STACT	Q5HMD0, THIE_STAEQ
Q8CNK2, THIE_STAES	Q4L7X3, THIE_STAHJ	Q49Z39, THIE_STAS1
Q3K1L6, THIE_STRA1	Q8E5W9, THIE_STRA3	Q8E092, THIE_STRA5
Q82AF9, THIE_STRAW	Q9S2V2, THIE_STRCO	A4W0K4, THIE_STRS2
A4VQX9, THIE_STUS1	A5GMH7, THIE_SYNPW	Q0IC17, THIE_SYNS3
Q3AL68, THIE_SYNSC	Q0AZ86, THIE_SYNWW	P72965, THIE_SYNY3
Q9HIQ4, THIE_THEAC	Q47R33, THIE_THEFY	Q72KL8, THIE_THET2
Q5SKG9, THIE_THET8	Q8DHK2, THIE_THEVB	Q97BE8, THIE_THEVO
Q3SFU7, THIE_THIDA	B8GLE3, THIE_THISH	A7N7S3, THIE_VIBC1
Q87JW8, THIE_VIBPA	Q7M9N9, THIE_WOLSU	Q8PH45, THIE_XANAC
Q4UYA4, THIE_XANC8	Q8P5R7, THIE_XANCP	A7IGG9, THIE_XANP2
Q9PGC4, THIE_XYLFA	Q87AX6, THIE_XYLFT	A7FNH7, THIE_YERP3
Q1C1U8, THIE_YERPA	Q8ZAQ1, THIE_YERPE	Q1CN71, THIE_YERPN
A4TS25, THIE_YERPP	Q66FP6, THIE_YERPS	Q67T51, THIME_SYMTH
O26949, THIN_METTH	Q5M731, TPS1L_ARATH	O48881, TPS1_BRANA
Q2QWK9, TPS1_ORYSJ

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