ID 2.4.1.341 DE alpha-1,2-colitosyltransferase. CA GDP-beta-L-colitose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine = CA alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine CA + GDP + H(+). CC -!- The enzyme, characterized from the bacterium Escherichia coli O55:H7, CC participates in the biosynthesis of an O-antigen. CC -!- The reaction involves anomeric inversion, and does not require any CC metal ions. CC -!- The enzyme is highly specific toward the acceptor, exclusively CC recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor CC with almost the same activity as with GDP-beta-L-colitose. DR Q8VQ46, WBGN_ECOLX ; //