Time sensitive network requires ultra-low delay time services. It is necessary to know the state of the transmission paths, especially to be able to quickly detect and locate fault point when a fault occurs. The fault detection and location method based on segment routing is studied in this paper. A candidate probe paths set generation algorithm based on segment routing is proposed, and an optimal probe matrix construction algorithm is designed. According to the information about the probe paths in the probe matrix, the fault node can be detected and located quickly. Due to the minimization of the number of the probe paths, the detection process does not occupy too much bandwidth resources, and the time is short, which can meet the needs of time sensitive network.