Hort. Sci. (Prague), 2011, 38(1):1-10 | DOI: 10.17221/42/2010-HORTSCI
Changes in phenols composition and activity of phenylalanine-ammonia lyase in apples after fungal infections
- Department of Food Preservation and Meat Technology, Institute of Chemical Technology, Prague, Czech Republic
The defensive reaction of apple cultivar Idared (Malus domestica Borkh.) was studied after inoculation with three different pathogens (Penicillium expansum, Monilinia fructigena, and Gloeosporium spp.). Changes in phenolic content and activity of phenylalanine-ammonia lyase were determined after 7, 14, and 21 days after the inoculation. The significant differences were discovered in the progress of rotting after the inoculation. The increase in phenols concentration and in phenylalanine-ammonia lyase activity varied in the place of fungal attack, in the tissues around rotten zone and in the healthy part. The response to the infection was different in the fruit peel and flesh. Very good correlation was found between the activity of phenylalanine-ammonia lyase and total phenol content (r = 0.76-0.98).
Keywords: apples; postharvest rot; fungal pathogens; phenolic compounds; phenylalanine-ammonia lyase
Published: March 31, 2011 Show citation
References
- Adámkova Š., Luhová L., Petivalský M., Pec P., 2006. Role L-fenylalaninamoniumlyasy při obranné reakci rostlin (Characterization of enzyme phenylalanine ammonialyase and its role in activation of defensive mechanisms in plants). Chemické Listy, 100: 486-494.
- Baert K., Devlieghere F., Flyps H., Oosterlinck M., Ahmed M.M., Rajkovi A., Verlinden B., Nicola B., Debevere J., De Meulenaer B., 2007. Influence of storage conditions of apples on growth and patulin production by Penicillium expansum. International Journal of Food Microbiology, 119: 170-181.
Go to original source...
Go to PubMed...
- Blažek J., Kloutvorová J., Kelinová J., 2006. Incidence of storage diseases on apples of selected cultivars and advanced selections grown with and without fungicide treatments. Horticultural Science (Prague), 33: 87-94.
Go to original source...
- Camm E.L., Towers G.H.N., 1973. Phenylalanine ammonia lyase. Phytochemistry, 12: 961-973.
Go to original source...
- Cantos E., Tudela J.A., Gil M.I., Espiän J.C., 2002. Phenolic compounds and related enzymes are not rate-limiting in browning development of fresh-cut potatoes. Journal of Agricultural and Food Chemistry, 50: 3015-3023.
Go to original source...
Go to PubMed...
- Deacon J.W., 2006. Fungal Biology. 4th Ed. Oxford, Blackwall Publishing, Ltd.: 279.
Go to original source...
- Dennis C., 1983. Post-harvest pathology of fruits and vegetables. London, Academic Press Inc.
- Escarpa A., González M.C., 1998. High-performance liquid chromatography with diode-array detection for the determination of phenolic compounds in peel and pulp from different apple varieties. Journal of Chromatography A, 823: 331-337.
Go to original source...
Go to PubMed...
- Escarpa A., González M.C., 2001. Approach to the content of total extractable phenolic compounds from different food samples by comparison of chromatographic and spectrophotometric methods. Analytica Chimica Acta, 427: 119-127.
Go to original source...
- Homutová I., Blažek J., 2006. Differences in fruit skin thickness between selected apple (Malus domestica Borkh.) cultivars assessed by histological and sensory methods. Horticultural Science (Prague), 33: 108-113.
Go to original source...
- Jesenská Z., 1993. Micromycetes in foodstuffs and feedstuffs. Amsterdam, Elsevier: 72.
- Jones D.H., 1984. Phenylalanine ammonia-lyase: Regulation of its induction, and its role in plant development. Phytochemistry, 23: 1349-1359.
Go to original source...
- Jones H., 1984. Phenylalanine ammonia-lyase: Regulation of its induction, and its role in plant development. Phytochemistry, 23: 1349-1359.
Go to original source...
- Łata B., 2007. Relationship between apple peel and the whole fruit antioxidant content: year and cultivar variation. Journal of Agricultural and Food Chemistry, 55: 663-671.
Go to original source...
Go to PubMed...
- Lattanzio V., Di Venere D., Linsalata V., Bertolini P., 2001. Low temperature metabolism of apple phenolics and quiescence of Phlyctaena vagabunda. Journal of Agricultural and Food Chemistry, 49: 5817-5821.
Go to original source...
Go to PubMed...
- Lattanzio V., Lattanzio V.M.T., Cardinali A., 2006. Role of phenolics in the resistance mechanisms of plant against fungal pathogens and insects. Phytochemistry: Advances in Research. Kerala, India, Research Signpost: 23-67.
- Mikuli Petkovšek M., Usenik V., Štampar F., 2003. The role of chlorogenic acid in the resistance of apples to apple scab (Venturia inaequalis (Cooke) G. Wind. Aderh.). In: Zbornik Biotehniške fakultete Univerze v Ljubljani. Kmetijstvo, Univerza v Ljubljani, 81: 233-242.
Go to original source...
- Mikuli Petkovšek M., Stampar F., Veberic R., 2007. Parameters of inner quality of the apple scab resistant and susceptible apple cultivars (Malus domestica Borkh.). Scientia Horticulturae, 114: 37-44.
Go to original source...
- Mikuli Petkovšek M., Stampar F., Veberic R., 2008. Increased phenolic content in apple leaves infected with the apple scab pathogen. Journal of Plant Pathology, 90: 49-55.
Go to original source...
- Mikuli Petkovšek M., Stampar F., Veberic R., 2009. Accumulation of phenolic compounds in apple in response to infection by the scab pathogen, Venturia inaequalis. Physiological and Molecular Plant Pathology, 74: 60-67.
Go to original source...
- Mikuli Petkovšek M., Slatnar A., Stampar F., Veberic R., 2010. The influence of organic/integrated production on the content of phenolic compounds in apple leaves and fruits in four different varieties over a 2-year period. Journal of the Science of Food and Agriculture. (in Press)
- Morelló J.-M., Romero M.-P., Ramo T., Motilva M.-J., 2005. Evaluation of L-Phenylalanine ammonia-lyase activity and phenolic profile in olive drupe (Olea europaea L.) from fruit setting period to harvesting time. Plant Science, 168: 65-72.
Go to original source...
- Pereyra L., Roura S.I., Del Valle C.E., 2005. Phenylalanine ammonia lyase activity in minimally processed Romaine lettuce. Lebensmittel Wissenschaft und Technologie, 38: 67-72.
Go to original source...
- Prusky D., 1996. Pathogen quiescence in postharvest diseases. Annual Review of Phytopathology, 34: 413-434.
Go to original source...
Go to PubMed...
- Slatnar A., Mikuli Petkovšek M., Halbwirth H., Stampar F., Stich K., Veberic R., 2010. Enzyme activity of the phenylpropanoid pathway as a response to apple scab infection. Annals of Applied Biology, 156: 449-456.
Go to original source...
- Spotts R.A., 1985. Effect of preharvest pear fruit maturity on decay resistance. Plant Disease, 69: 388-390.
Go to original source...
- Torres R., Valentines M.C., Usall J., Vias I., Larrigaudiere C., 2003. Possible involvement of hydrogen peroxide in the development of resistance mechanisms in ´Golden Delicious´ apple fruit. Postharvest Biology and Technology, 27: 235-242.
Go to original source...
- Tsao R., Yang R., Young J. C., Zhu H., 2003. Polyphenolic profiles in eight apple cultivars using high-performance liquid chromatography (HPLC). Journal of Agricultural and Food Chemistry, 51: 6347-6353.
Go to original source...
Go to PubMed...
- Veberic R., Trobec M., Hofer M., Grill D., Stampar F., 2005. Phenolic compounds in some apple (Malus domestica Borkh.) cultivars of organic and integrated production. Journal of the Science of Food and Agriculture, 85: 1687-1694.
Go to original source...
- Zhi-Guo J., Yong-Bing Y., Cheng-Lian L., Shi-Hai X., 1995. Relationships among phenylalanine ammonia-lyase activity, simple phenol concentrations and anthocyanin accumulation in apple. Scientia Horticulturae, 61: 215-226.
Go to original source...
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.