This paper proposes a method for cooperative multi-static Multiple Input Multiple Output (MIMO) radar that can estimate the number of targets. The purpose of this system is to monitor humans in an indoor environment. First, target positions within the estimation range are roughly detected by the Capon method and the mode vector corresponding to the detected positions is calculated. The mode vector is multiplied by the eigenvector to eliminate the virtual image. The spectrum of the evaluation function is calculated from the remaining positions, and the number of peaks in the spectrum is defined as the number of targets. Experiments carried out in an indoor environment confirm that the proposed method can estimate the number of targets with high accuracy.