EP 0770144 A1 19970502 - NUCLEIC ACID AMPLIFICATION METHOD USING A MODIFIED NUCLEOSIDE, AND DETECTION OF THE AMPLIFICATION PRODUCT USING ANTIBODIES
Title (en)
NUCLEIC ACID AMPLIFICATION METHOD USING A MODIFIED NUCLEOSIDE, AND DETECTION OF THE AMPLIFICATION PRODUCT USING ANTIBODIES
Title (de)
VERFAHREN ZUR AMPLIFIKATION VON NUKLEINSÄUREN UNTER VERWENDUNG VON EINEM MODIFIZIERTEN NUKLEOSID UND NACHWEIS VON EINEM PRODUKT DER AMPLIFIKATION MIT ANTIKÖRPERN
Title (fr)
PROCEDE D'AMPLIFICATION D'ACIDE NUCLEIQUE A L'AIDE D'UN NUCLEOSIDE MODIFIE, ET DETECTION DU PRODUIT D'AMPLIFICATION A L'AIDE D'ANTICORPS
Publication
Application
Priority
- FR 9500985 W 19950721
- FR 9409041 A 19940721
Abstract (en)
[origin: WO9603526A1] A nucleotide sequence amplification method wherein a polynucleotide containing the sequence is contacted with oligonucleotide primers, nucleoside triphosphates and/or deoxynucleoside triphosphates containing the various purine and pyrimidine bases needed to synthesise the amplification product, and an enzyme system having DNA polymerase and/or RNA polymerase and/or reverse transcriptase activity, under conditions enabling the sequence of interest to be amplified in a per se known manner. One of the nucleoside triphosphates and/or deoxynucleoside triphosphates is present either in a modified form or in both a modified form and an unmodified form, and, in said modified form, the purine or pyrimidine base is substituted by at least one methyl, hydroxymethyl or acetyl grouping.
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6804 (2018.01); C12Q 1/686 (2018.01)
CPC (source: EP)
C12Q 1/6804 (2013.01); C12Q 1/686 (2013.01)
Citation (search report)
See references of WO 9603526A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB IT LI NL SE
DOCDB simple family (publication)
WO 9603526 A1 19960208; AU 3081495 A 19960222; CA 2195480 A1 19960208; EP 0770144 A1 19970502; FR 2722799 A1 19960126; FR 2722799 B1 19961004; JP H10505230 A 19980526
DOCDB simple family (application)
FR 9500985 W 19950721; AU 3081495 A 19950721; CA 2195480 A 19950721; EP 95926413 A 19950721; FR 9409041 A 19940721; JP 50552595 A 19950721