Effect of dehydroepiandrosterone on meiotic spindle structure and oocyte quality in mice
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 21، شماره: 10
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 216
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شناسه ملی سند علمی:
JR_IJBMS-21-10_006
تاریخ نمایه سازی: 27 مهر 1400
چکیده مقاله:
Objective(s): Dehydroepiandrosterone (DHEA) has been reported to improve pregnancy chances in women with diminished ovarian reserve (DOR) and to reduce miscarriage rates by ۵۰–۸۰%. This study, therefore, assesses effects of DHEA on number of retrieved oocytes and meiotic spindles.Materials and Methods: A randomized, prospective, controlled study was conducted on eight groups, four groups of young mice and four elderly. All young and old groups received different oral doses (۳۵, ۵۰, ۷۵ mg/kg) of DHEA for ۳ months. Meiotic spindle assessment was done by immunocytochemical techniques using a confocal laser microscope (Leica TCS-۴D).Results: Statistical surveys showed that in control young groups ۸۰% (P=۰.۰۸۴۵) and in the old control group ۷۳.۳% (P=۰.۰۰۰) of the meiotic spindles have a normal shape and structure; the difference was meaningful. The young with ۵۰ mg/kg of DHEA in ۸۵.۴% and the young with ۷۵ mg/kg of DHEA in ۸۴.۲% were normal in shape and structure. Statistical analysis showed that the difference was meaningless (P=۰.۸۴۵). The old group with ۳۰ mg/kg of DHEA in ۸۱.۱%, the old with ۵۰ mg/kg of DHEA in ۸۳.۹%, and the old with ۷۵ mg/kg of DHEA in ۷۹.۰% showed normal shape and structure. The meiotic spindle disruption ratio in old mice showed a significant difference (P=۰.۰۰۰) in comparison with others in young groups. Statistical analysis showed that difference between DHEA and control groups is meaningful. But this difference was meaningless between DHEA groups.Conclusion: Results showed that DHEA has a positive and improvement effect on the meiotic spindle in old mice.
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نویسندگان
Ali Reza Eftekhari moghadam
Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapour University of Medical Science, Ahvaz, Iran
Ghasem Saki
Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
Masoud Hemadi
Fertility and Infertility Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Zohre Mazaheri
Department of Anatomical Science, Faculty of Medicine, Tarbiat Modarres University, Tehran, Iran
Ali Khodadadi
Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
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