TRPV۱ receptor-mediated expression of Toll-like receptors ۲ and ۴ following permanent middle cerebral artery occlusion in rats

محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 20، شماره: 8

سال انتشار: 1396

نوع سند: مقاله ژورنالی

زبان: انگلیسی

مشاهده: 248

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https://civilica.com/doc/1295677

شناسه ملی سند علمی:

JR_IJBMS-20-8_004

تاریخ نمایه سازی: 28 مهر 1400

چکیده مقاله:

Objective(s): Stroke is known as a main cause of mortality and prolonged disability in adults. Both transient receptor potential V۱ (TRPV۱) channels and toll-like receptors (TLRs) are involved in mediating the inflammatory responses. In the present study, the effects of TRPV۱ receptor activation and blockade on stroke outcome and gene expression of TLR۲ and TLR۴ were assessed following permanent middle cerebral artery occlusion in rats Materials and Methods: Eighty male Wistar rats were divided into four groups as follows: sham, vehicle, AMG۹۸۱۰ (TRPV۱ antagonist) -treated and capsaicin (TRPV۱ agonist) -treated. For Stroke induction, the middle cerebral artery was permanently occluded and then behavioral functions were evaluated ۱, ۳ and ۷ days after stroke. Results: TRPV۱ antagonism significantly reduced the infarct volume compared to the stroke group. Also, neurological deficits were decreased by AMG۹۸۱۰ seven days after cerebral ischemia. In the ledged beam-walking test, the slip ratio was enhanced following ischemia. AMG۹۸۱۰ decreased this index in stroke animals. However, capsaicin improved the ratio ۳ and ۷ days after cerebral ischemia. Compared to the sham group, the mRNA expression of TLR۲ and TLR۴ was significantly increased in the stroke rats. AMG۹۸۱۰ Administration significantly reduced the mRNA expression of TLR۲ and TLR۴. However, capsaicin did not significantly affect the gene expression of TLR۲ and TLR۴. Conclusion: Our results demonstrated that TRPV۱ antagonism by AMG۹۸۱۰ attenuates behavioral function and mRNA expression of TLR۲ and TLR۴. Thus, it might be useful to shed light on future therapeutic strategies for the treatment of ischemic stroke.

کلیدواژه ها:

Cerebral ischemia ، Inflammation ، TLR۴ ، TLR۲ ، TRPV۱

نویسندگان

Elham Hakimizadeh

Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Ali Shamsizadeh

Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Ali Roohbakhsh

Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

Mohammad Kazemi Arababadi

Department of Laboratory Sciences, School of Paramedicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Mohammad Reza Hajizadeh

Molecular Medicine Research Center, Department of Biochemistry, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Mehdi Shariati

Department of Anatomy, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Iman Fatemi

Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Amir Moghadam-ahmadi

Department of Neurology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Gholamreza Bazmandegan

Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Hossein Rezazadeh

Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

Mohammad Allahtavakoli

Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

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