Gestational diabetes mellitus increased the number of dopaminergic neurons in the olfactory bulb of rat offspring
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 26، شماره: 12
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 119
فایل این مقاله در 6 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-26-12_014
تاریخ نمایه سازی: 7 آبان 1402
چکیده مقاله:
Objective(s): Gestational Diabetes Mellitus (GDM) is the most common metabolic complication of pregnancy that causes central nervous system and olfactory dysfunction in the offspring. It has been demonstrated that dopamine modulates several aspects of olfactory information processing in vertebrates. Materials and Methods: In this study, we investigated the effect of gestational diabetes on the expression of the Dopamine (DA) metabolism genes, tyrosine hydroxylase (TH), and dopa decarboxylase (DDC) in the olfactory bulb (OB) tissue of rats’ offspring. Female Wistar rats were divided into a control group which received citrate buffer and the diabetic group which received ۴۵ mg/kg of streptozotocin (STZ) on day ۰ of gestation. Fasting blood glucose levels were measured before and ۷۲ hr after injection. OB tissues of adult offspring were isolated, and TH-positive cells were counted by immunofluorescence staining. Also, TH and DDC expressions were analyzed by qRT- PCR and western blot.Results: The data showed that gestational diabetes could cause up-regulation of TH (P<۰.۰۱) and DDC (P<۰.۰۵) in the OB tissue of offspring. Furthermore, our results showed that GDM causes a significant increase in TH and DDC protein levels in the OB tissues of offspring. Immunohistochemistry showed a significant increase in the number of TH-positive cells in the offspring of diabetic mothers (P<۰.۰۵).Conclusion: This study showed that gestational diabetes could cause an increase in TH and DDC gene expression in the OB tissue in the offspring, which may be correlated with reduced olfactory sensitivity.
کلیدواژه ها:
نویسندگان
Zahra Nazari
Department of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran
Khadijeh Bahrehbar
Department of Biology, Faculty of Basic Sciences, Yasuj University, Yasuj, Iran
Soraya Ghafari
Congenital Malformations Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Mohammad Jafar Golalipour
Congenital Malformations Research Center, Golestan University of Medical Sciences, Gorgan, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :