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Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Nitrogen and sulfur uptake and remobilisation in canola genotypes with varied N- and S-use efficiency differ at vegetative and maturity stages

Tatjana Balint A and Zdenko Rengel A B
+ Author Affiliations
- Author Affiliations

A School of Earth and Environment, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B Corresponding author. Email: zed.rengel@uwa.edu.au

Crop and Pasture Science 62(4) 299-312 https://doi.org/10.1071/CP10272
Submitted: 19 August 2010  Accepted: 7 March 2011   Published: 19 April 2011

Abstract

Eight canola genotypes chosen from a preliminary screening study with either high or low nitrogen (N) and/or sulfur (S) efficiency indices were tested for consistency in S and/or N efficiency between vegetative stage and maturity. Soil labelling was used to assess 15N/N or 34S/S uptake, and a leaf feeding–labelling technique was used to study transport and remobilisation of N and S. Plants were grown under glasshouse conditions using deficient or adequate N and/or S supply. Nitrogen and S uptake in plants was assessed using the following criteria: growth, N and/or S concentration, and 15N and/or 34S atom excess in above-ground plant parts. Transport and remobilisation of N and S were assessed via the same assessment criteria in plants at 2 days (1st harvest) and 6 days (2nd harvest) after commencement of leaf-labelling.

Genotype Wesway was more efficient than Westar in taking up N during the vegetative stage as well as in remobilising N from leaves, stems, and siliques to seeds at maturity. Genotype Surpass 402 CL appeared to be more efficient than Karoo in taking up S during the vegetative stage and particularly during the maturity stage, but was less efficient than Karoo in remobilising S from leaves and stems to siliques and seeds at maturity. Soil and leaf labelling techniques using 15N and 34S appeared to be useful tools for studying uptake, transport, and remobilisation of N and S during the vegetative and maturity stages in canola genotypes differing in N- or S-use efficiency.

Additional keywords: canola, leaf labelling, 15N, remobilisation, 34S, soil labelling.


References

Abdallah M, Dubousset L, Meuriot F, Etienne P, Avice JC, Ourry A (2010) Effect of mineral sulphur availability on nitrogen and sulphur uptake and remobilization during the vegetative growth of Brassica napus L. Journal of Experimental Botany 61, 2635–2646.
Effect of mineral sulphur availability on nitrogen and sulphur uptake and remobilization during the vegetative growth of Brassica napus L.Crossref | Brassica napus L.&journal=Journal of Experimental Botany&volume=61&pages=2635-2646&publication_year=2010&author=M%20Abdallah&hl=en&doi=10.1093/jxb/erq096" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXnsVOgt7s%3D&md5=71ffb817b30d6af836bd4f7e447b5fcfCAS | 20403880PubMed |

Ahmad A, Khan I, Nasar AA, Abrol YP, Iqbal M (2005) Role of sulphate transporter system in sulphur efficiency of mustard genotypes. Plant Science 169, 842–846.
Role of sulphate transporter system in sulphur efficiency of mustard genotypes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXpslChur4%3D&md5=2a662c75f6f61e7326a1b6fbc05e4eaaCAS |

Balint T, Rengel Z (2008) Nitrogen efficiency of canola genotypes varies between vegetative stage and grain maturity. Euphytica 164, 421–432.
Nitrogen efficiency of canola genotypes varies between vegetative stage and grain maturity.Crossref | GoogleScholarGoogle Scholar |

Balint T, Rengel Z (2009) Differential sulfur efficiency in canola genotypes at vegetative and grain maturity stage. Crop & Pasture Science 60, 262–270.
Differential sulfur efficiency in canola genotypes at vegetative and grain maturity stage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXjtF2htbo%3D&md5=9a878697f80ae0646bfe07bdb27f128fCAS |

Balint T, Rengel Z (2011) Amino acid composition of xylem and phloem sap varies in canola genotypes differing in nitrogen- and sulfur-use efficiency. Crop & Pasture Science 62, 198–207.
Amino acid composition of xylem and phloem sap varies in canola genotypes differing in nitrogen- and sulfur-use efficiency.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjt12ktbw%3D&md5=4994176512892ba4e114aa236c30fee2CAS |

Balint T, Rengel Z, Allen D (2008) Australian canola germplasm differs in nitrogen and sulfur efficiency. Australian Journal of Agricultural Research 59, 167–174.
Australian canola germplasm differs in nitrogen and sulfur efficiency.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXitVGhsbs%3D&md5=e8adb39d3f21d7929e49043533bf2b4fCAS |

Barbottin A, Lecomte C, Bouchard C, Jeuffroy MH (2005) Nitrogen remobilization during grain filling in wheat: genotypic and environmental effects. Crop Science 45, 1141–1150.
Nitrogen remobilization during grain filling in wheat: genotypic and environmental effects.Crossref | GoogleScholarGoogle Scholar |

Bremner JM (1990) Problems in the use of urea as a nitrogen fertilizer. Soil Use and Management 6, 70–71.
Problems in the use of urea as a nitrogen fertilizer.Crossref | GoogleScholarGoogle Scholar |

Buchner P, Stuiver CEE, Westerman S, Wirtz M, Hell R, Hawkesford MJ, De Kok LJ (2004) Regulation of sulfate uptake and expression of sulfate transporter genes in Brassica oleracea as affected by atmospheric H2S and pedospheric sulfate nutrition. Plant Physiology 136, 3396–3408.
Regulation of sulfate uptake and expression of sulfate transporter genes in Brassica oleracea as affected by atmospheric H2S and pedospheric sulfate nutrition.Crossref | Brassica oleracea as affected by atmospheric H2S and pedospheric sulfate nutrition.&journal=Plant Physiology&volume=136&pages=3396-3408&publication_year=2004&author=P%20Buchner&hl=en&doi=10.1104/pp.104.046441" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXovVymsbs%3D&md5=5d5e0d55353a1dd285f8d5a3ee3cc301CAS | 15377780PubMed |

Diaz C, Lemaître T, Christ C, Azzopardi M, Kato Y, Sato F, Morot-Gaudry JF, Le Dily F, Masclaux-Daubresse C (2008) Nitrogen recycling and remobilization are differentially controlled by leaf senescence and development stage in Arabidopsis under low nitrogen nutrition. Plant Physiology 147, 1437–1449.
Nitrogen recycling and remobilization are differentially controlled by leaf senescence and development stage in Arabidopsis under low nitrogen nutrition.Crossref | Arabidopsis under low nitrogen nutrition.&journal=Plant Physiology&volume=147&pages=1437-1449&publication_year=2008&author=C%20Diaz&hl=en&doi=10.1104/pp.108.119040" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXoslyisbY%3D&md5=7dae1bc75e82bb5823926943ee720836CAS | 18467460PubMed |

Dreccer MF, Schapendonk AHM, Slafer GA, Rabbinge R (2000) Comparative response of wheat and oilseed rape to nitrogen supply: absorption and utilisation efficiency of radiation and nitrogen during the reproductive stage determining yield. Plant and Soil 220, 189–205.
Comparative response of wheat and oilseed rape to nitrogen supply: absorption and utilisation efficiency of radiation and nitrogen during the reproductive stage determining yield.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXks1Kltrg%3D&md5=1be6f90d521ade20a2b720fce237f3aaCAS |

Dubousset L, Abdallah M, Desfeux AS, Etienne P, Meuriot F, Hawkesford MJ, Gombert J, Segura R, Bataille M-P, Reze S, Bonnefoy J, Ameline AF, Ourry A, Le Dily F, Avice JC (2009) Remobilization of leaf S compounds and senescence in response to restricted sulphate supply during the vegetative stage of oilseed rape are affected by mineral N availability. Journal of Experimental Botany 60, 3239–3253.
Remobilization of leaf S compounds and senescence in response to restricted sulphate supply during the vegetative stage of oilseed rape are affected by mineral N availability.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXpsValtro%3D&md5=4433e22812c6ab5757db9e4d54961383CAS | 19553370PubMed |

Fismes J, Vong PC, Guckert A (1999) Use of labeled sulphur-35 for tracing sulphur transfers in developing pods of field-grown oilseed rape. Communications in Soil Science and Plant Analysis 30, 221–234.
Use of labeled sulphur-35 for tracing sulphur transfers in developing pods of field-grown oilseed rape.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXhtlGiu78%3D&md5=77817e29c64fc142535564c72e0bbbf5CAS |

Gabrielle B, Denoroy P, Gosse G, Justes E, Andersen MN (1998) Development and evaluation of a CERES-type model for winter oilseed rape. Field Crops Research 57, 95–111.
Development and evaluation of a CERES-type model for winter oilseed rape.Crossref | GoogleScholarGoogle Scholar |

Gombert J, Le Dily F, Lothier J, Etienne P, Rossato L, Allirand JM, Jullien A, Savin A, Ourry A (2010) Effect of nitrogen fertilization on nitrogen dynamics in oilseed rape using 15N-labeling field experiment. Journal of Plant Nutrition and Soil Science 173, 875–884.
Effect of nitrogen fertilization on nitrogen dynamics in oilseed rape using 15N-labeling field experiment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsVyrtr%2FI&md5=12158b070a9d80ed68de17f2d7c9c63fCAS |

Greenwood DJ, Gastal F, Lemaire G, Drycott A, Millard P, Neeteson JJ (1991) Growth rate and % N of field grown crops: theory and experiments. Annals of Botany 67, 181–190.

Hawkesford MJ, DeKok LJ (2006) Managing sulfur metabolism in plants. Plant, Cell & Environment 29, 382–395.
Managing sulfur metabolism in plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xktlyltrg%3D&md5=6d77198f4a7f44ee7d91bd1c339d2b23CAS | 17080593PubMed |

Herschbach C, De Kok LJ, Rennenberg H (1995a) Net uptake of sulfate and its transport to the shoot in spinach plants fumigated with H2S or SO2: does atmospheric sulfur affect the ‘inter-organ’ regulation of sulfur nutrition. Botanica Acta 108, 41–46.

Herschbach C, De Kok LJ, Rennenberg H (1995b) Net uptake of sulfate and its transport to the shoot in tobacco plants fumigated with H2S or SO2. Plant and Soil 175, 75–84.

Herschbach C, Pilch B, Tausz M, Rennenberg H, Grill D (2002) Sulphate uptake and xylem loading of young pea (Pisum sativum L.) seedlings. Plant and Soil 242, 227–233.
Sulphate uptake and xylem loading of young pea (Pisum sativum L.) seedlings.Crossref | Pisum sativum L.) seedlings.&journal=Plant and Soil&volume=242&pages=227-233&publication_year=2002&author=C%20Herschbach&hl=en&doi=10.1023/A:1016205905244" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XltFKisb0%3D&md5=8539afdfaf8322817b43883b2026a0f2CAS |

Hocking PJ, Randall PJ, DeMarco D (1997) The response of dryland canola to nitrogen fertilizer: partitioning and remobilization of dry matter and nitrogen, and nitrogen effects on yield components. Field Crops Research 54, 201–220.
The response of dryland canola to nitrogen fertilizer: partitioning and remobilization of dry matter and nitrogen, and nitrogen effects on yield components.Crossref | GoogleScholarGoogle Scholar |

Krogmeier MJ, McCarty GW, Bremner JM (1989) Phytotoxicity of foliar-applied urea. Proceedings of the National Academy of Sciences of the United States of America 86, 8189–8191.
Phytotoxicity of foliar-applied urea.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXitVeqtA%3D%3D&md5=651ce31d84e8c37a845b1c62757d7680CAS | 16594077PubMed |

Lexa M, Cheeseman JM (1997) Growth and nitrogen relations in reciprocal grafts of wild-type and nitrate reductase-deficient mutants of pea (Pisum sativum L. var. Juneau). Journal of Experimental Botany 48, 1241–1250.
Growth and nitrogen relations in reciprocal grafts of wild-type and nitrate reductase-deficient mutants of pea (Pisum sativum L. var. Juneau).Crossref | Pisum sativum L. var. Juneau).&journal=Journal of Experimental Botany&volume=48&pages=1241-1250&publication_year=1997&author=M%20Lexa&hl=en&doi=10.1093/jxb/48.6.1241" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXltlSrsb4%3D&md5=cb9a31bc3315f74329e3ade83bd11ba2CAS |

Malagoli P, Laine’ P, Le Deunff E, Rossato L, Ney B, Ourry A (2004) Modeling N uptake in Brassica napus L. cv. Capitol during a growth cycle using influx kinetics of root nitrate transport systems and field experimental data. Plant Physiology 134, 388–400.
Modeling N uptake in Brassica napus L. cv. Capitol during a growth cycle using influx kinetics of root nitrate transport systems and field experimental data.Crossref | Brassica napus L. cv. Capitol during a growth cycle using influx kinetics of root nitrate transport systems and field experimental data.&journal=Plant Physiology&volume=134&pages=388-400&publication_year=2004&author=P%20Malagoli&hl=en&doi=10.1104/pp.103.029538" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhtVansrs%3D&md5=fa2e0ffecc1634dca0914e694171ba76CAS | 14671012PubMed |

Malagoli P, Laine P, Rossato L, Ourry A (2005) Dynamics of nitrogen uptake and mobilization in field-grown winter oilseed rape (Brassica napus) from stem extension to harvest. Global N flows between vegetative and reproductive tissues in relation to leaf fall and their residual N. Annals of Botany 95, 853–861.
Dynamics of nitrogen uptake and mobilization in field-grown winter oilseed rape (Brassica napus) from stem extension to harvest. Global N flows between vegetative and reproductive tissues in relation to leaf fall and their residual N.Crossref | Brassica napus) from stem extension to harvest. Global N flows between vegetative and reproductive tissues in relation to leaf fall and their residual N.&journal=Annals of Botany&volume=95&pages=853-861&publication_year=2005&author=P%20Malagoli&hl=en&doi=10.1093/aob/mci091" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjvFOrtr0%3D&md5=ad4766a6089e12f8a1aa081ee71a072aCAS | 15701662PubMed |

Malhi SS, Gan Y, Raney JP (2007) Yield, seed quality, and sulfur uptake of Brassica oilseed crops in response to sulfur fertilization. Agronomy Journal 99, 570–578.
Yield, seed quality, and sulfur uptake of Brassica oilseed crops in response to sulfur fertilization.Crossref | Brassica oilseed crops in response to sulfur fertilization.&journal=Agronomy Journal&volume=99&pages=570-578&publication_year=2007&author=SS%20Malhi&hl=en&doi=10.2134/agronj2006.0269" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXkslymsbk%3D&md5=4071180aeb0d1a35574b1681a38a2088CAS |

McNeill AM, Zhua CH, Fillery IRP (1997) Use of in situ 15N-labelling to estimate the total below-ground nitrogen of pasture legumes in intact soil–plant systems. Australian Journal of Agricultural Research 48, 295–304.
Use of in situ 15N-labelling to estimate the total below-ground nitrogen of pasture legumes in intact soil–plant systems.Crossref | in situ 15N-labelling to estimate the total below-ground nitrogen of pasture legumes in intact soil–plant systems.&journal=Australian Journal of Agricultural Research&volume=48&pages=295-304&publication_year=1997&author=AM%20McNeill&hl=en&doi=10.1071/A96097" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar |

Mickelson S, See D, Meyer FD, Garner JP, Foster CR, Blake TK, Fischer AM (2003) Mapping of QTL associated with nitrogen storage and remobilization in barley (Hordeum vulgare L.) leaves. Journal of Experimental Botany 54, 801–812.
Mapping of QTL associated with nitrogen storage and remobilization in barley (Hordeum vulgare L.) leaves.Crossref | Hordeum vulgare L.) leaves.&journal=Journal of Experimental Botany&volume=54&pages=801-812&publication_year=2003&author=S%20Mickelson&hl=en&doi=10.1093/jxb/erg084" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXit1aru7k%3D&md5=8744551e2f3d645723498eb4a9fb9bacCAS | 12554723PubMed |

Möllers C, Bolune T, Wallbraun M, Lohaus G (1996) Protein content and amino acid composition of the seeds and the phloem sap of one Brassica carinata and different B. napus L. genotypes. In ‘Proceedings of the Symposium Breeding for Oil and Protein Crops’. Zaporozhye, Ukraina. pp. 148–154.

Möllers C, Schierholt A (2002) Genetic variation of palmitate and oil content in a winter oilseed rape doubled haploid population segregating for oleate content. Crop Science 42, 379–384.
Genetic variation of palmitate and oil content in a winter oilseed rape doubled haploid population segregating for oleate content.Crossref | GoogleScholarGoogle Scholar |

Moore G (Ed.) (1998) ‘Soil guide: a handbook for understanding and managing agricultural soils.’ Agriculture Western Australia Bulletin 4343. (Lamb Print: Perth, W. Aust.)

Northcote KH (1971) ‘A factual key for the recognition of Australian soils.’ (Rellim: Glenside, S. Aust.)

Norton R, Burton W, Salisbury P (2004) Canola quality Brassica juncea for Australia. In ‘Proceeding of 4th International Crop Science Congress’. Brisbane, Qld. Poster 5.1.3. (Eds T Fischer, J Angus, L McIntyre, M Robertson, A Borrell, D Lloyd)

Palta JA, Fillery IR, Mathews EL, Turner NC (1991) Leaf feeding of [15N] urea for labelling wheat with nitrogen. Australian Journal of Plant Physiology 18, 627–636.
Leaf feeding of [15N] urea for labelling wheat with nitrogen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XitFSmur0%3D&md5=5f0e7748beb23dc0d97fcc68e4ff3e18CAS |

Paul D, Skrzypek G, Forizs I (2007) Normalization of measured stable isotopic compositions to isotope reference scales – a review. Rapid Communications in Mass Spectrometry 21, 3006–3014.
Normalization of measured stable isotopic compositions to isotope reference scales – a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtVyksL7L&md5=5fe40de839e64f8c5600b85da5194d35CAS | 17705258PubMed |

Pessarakli M, Tucker TC (1985) Uptake of nitrogen-15 by cotton under salt stress. Soil Science Society of America Journal 49, 149–152.
Uptake of nitrogen-15 by cotton under salt stress.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXhtFagtb0%3D&md5=e7840b59d8e9466494c1022609a3afa3CAS |

Plénet D, Lemair G (2000) Relationships between dynamics of nitrogen uptake and dry matter accumulation in maize crops. Determination of critical N concentration. Plant and Soil 16, 65–82.

Robson AD, Osborne LD, Snowball K, Simmons WJ (1995) Assessing sulfur status in lupins and wheat. Australian Journal of Experimental Agriculture 35, 79–86.
Assessing sulfur status in lupins and wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXmsVGnurw%3D&md5=993b74a3cbeb9795c50c9c537ae435d8CAS |

Rossato L, Laine P, Ourry A (2001) Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: nitrogen fluxes within the plant and changes in soluble protein patterns. Journal of Experimental Botany 52, 1655–1663.
Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: nitrogen fluxes within the plant and changes in soluble protein patterns.Crossref | Brassica napus L. during the growth cycle: nitrogen fluxes within the plant and changes in soluble protein patterns.&journal=Journal of Experimental Botany&volume=52&pages=1655-1663&publication_year=2001&author=L%20Rossato&hl=en&doi=10.1093/jexbot/52.361.1655" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXlvVClsrg%3D&md5=7f785cec1cb44bf46d59db2300ac449eCAS | 11479330PubMed |

Rouached H Wirtz M Alary R Hell R Bulak A Davidian J-C Fourcroy P Berthomieu P (2008 ) Differential regulation of the expression of two high-affinity sulfate transporters, SULTR1.1 and SULTR1.2, in Arabidopsis . Plant Physiology 147 897 911

Schjoerring JK, Bock JGH, Gammelvind L, Jensen CR, Mogensen VO (1995) Nitrogen incorporation and remobilization in different components of field grown winter oil seed rape (Brassica napus L.) as affected by rate of nitrogen application and irrigation. Plant and Soil 177, 255–264.
Nitrogen incorporation and remobilization in different components of field grown winter oil seed rape (Brassica napus L.) as affected by rate of nitrogen application and irrigation.Crossref | Brassica napus L.) as affected by rate of nitrogen application and irrigation.&journal=Plant and Soil&volume=177&pages=255-264&publication_year=1995&author=JK%20Schjoerring&hl=en&doi=10.1007/BF00010132" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XhtlOhtrw%3D&md5=d406e31c9c05cec67f09f798b48546c8CAS |

Skrzypek G, Paul D (2006) δ13C analyses of calcium carbonate: comparison between the GasBench and elemental analyzer techniques. Rapid Communications in Mass Spectrometry 20, 2915–2920.
δ13C analyses of calcium carbonate: comparison between the GasBench and elemental analyzer techniques.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtVylsr%2FJ&md5=a9ee78813ffbef683c16cbcb9b895112CAS | 16941549PubMed |

Smith FW (2001) Sulphur and phosphorus transport systems in plants. Plant and Soil 232, 109–118.
Sulphur and phosphorus transport systems in plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXlsVCks7c%3D&md5=fcff2ce0e41d9722a025f3ca6742330aCAS |

Sunarpi A, Anderson JW (1997) Effect of nitrogen nutrition on remobilization of protein sulfur in the leaves of vegetative soybean and associated changes in soluble sulfur metabolites. Plant Physiology 115, 1671–1680.

Svecnjak Z, Rengel Z (2006) Canola cultivars differ in nitrogen utilization efficiency at vegetative stage. Field Crops Research 97, 221–226.
Canola cultivars differ in nitrogen utilization efficiency at vegetative stage.Crossref | GoogleScholarGoogle Scholar |

Unkovich MJ, Pate JS, Sanford P, Armstrong EL (1994) Potential precision of the δ 15N natural abundance method in field estimates of nitrogen fixation by crop and pasture legumes in S.W. Australia. Australian Journal of Agricultural Research 45, 119–132.
Potential precision of the δ 15N natural abundance method in field estimates of nitrogen fixation by crop and pasture legumes in S.W. Australia.Crossref | GoogleScholarGoogle Scholar |

Westerman S, DeKok LJ, Stulen I (2000) Interaction between metabolism of atmospheric H2S in the shoot and sulfate uptake by the roots of curly kale (Brassica oleracea L.). Physiologia Plantarum 109, 443–449.
Interaction between metabolism of atmospheric H2S in the shoot and sulfate uptake by the roots of curly kale (Brassica oleracea L.).Crossref | Brassica oleracea L.).&journal=Physiologia Plantarum&volume=109&pages=443-449&publication_year=2000&author=S%20Westerman&hl=en&doi=10.1034/j.1399-3054.2000.100411.x" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXlsl2isro%3D&md5=431e0bbdff515bbcc8525aef4ee70543CAS |

Yau SK, Thurling N (1987) Variation in nitrogen response among spring rape (Brassica napus) cultivars and its relationship to nitrogen uptake and utilization. Field Crops Research 16, 139–155.
Variation in nitrogen response among spring rape (Brassica napus) cultivars and its relationship to nitrogen uptake and utilization.Crossref | Brassica napus) cultivars and its relationship to nitrogen uptake and utilization.&journal=Field Crops Research&volume=16&pages=139-155&publication_year=1987&author=SK%20Yau&hl=en&doi=10.1016/0378-4290(87)90004-9" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar |

Yoshimoto N, Inoue E, Saito K, Yamaya T, Takahashi H (2003) Phloem-localizing sulfate transporter, Sultrl;3,- mediates re-distribution of sulfur from source to sink organs in Arabidopsis. Plant Physiology 131, 1511–1517.
Phloem-localizing sulfate transporter, Sultrl;3,- mediates re-distribution of sulfur from source to sink organs in Arabidopsis.Crossref | Sultrl;3,- mediates re-distribution of sulfur from source to sink organs in Arabidopsis.&journal=Plant Physiology&volume=131&pages=1511-1517&publication_year=2003&author=N%20Yoshimoto&hl=en&doi=10.1104/pp.014712" target="_blank" rel="nofollow noopener noreferrer" class="reftools">GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXjt1Sqsr0%3D&md5=b934657cf4d77ca10b8d959caeaad798CAS | 12692311PubMed |

Zhao FJ, Verkampen KCJ, Birdsey M, Blake-Kalff MMA, McGrath SP (2001) Use of the enriched stable isotope 34S to study sulphur uptake and distribution in wheat. Journal of Plant Nutrition 24, 1551–1560.
Use of the enriched stable isotope 34S to study sulphur uptake and distribution in wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXnt12htbc%3D&md5=e76a153aaae9945c87ce31a8a9dc9a2fCAS |