Abstract
CD8(+) T lymphocytes from individuals infected with human immunodeficiency virus-type 1 (HIV-1) secrete a soluble activity that suppresses infection by HIV-1. A protein associated with this activity was purified from the culture supernatant of an immortalized CD8(+) T cell clone and identified as the beta-chemokine macrophage-derived chemokine (MDC). MDC suppressed infection of CD8(+) cell-depleted peripheral blood mononuclear cells by primary non-syncytium-inducing and syncytium-inducing isolates of HIV-1 and the T cell line-adapted isolate HIV-1IIIB. MDC was expressed in activated, but not resting, peripheral blood mononuclear cells and binds a receptor on activated primary T cells. These observations indicate that beta-chemokines are responsible for a major proportion of HIV-1-specific suppressor activity produced by primary T cells.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antiviral Agents / immunology*
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Blotting, Northern
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CD8-Positive T-Lymphocytes / immunology*
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Calcium / blood
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Cell Line
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Cell Line, Transformed
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Cells, Cultured
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Chemokine CCL22
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Chemokines, CC / chemistry
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Chemokines, CC / immunology*
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Chemokines, CC / isolation & purification
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Chemokines, CC / metabolism
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HIV Core Protein p24 / biosynthesis
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HIV Infections / immunology
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HIV-1 / immunology*
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HIV-1 / physiology
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Humans
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / metabolism
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Leukocytes, Mononuclear / virology*
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Lymphocyte Activation
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Receptors, Chemokine / metabolism
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Receptors, HIV / metabolism
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T-Lymphocytes / immunology
Substances
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Antiviral Agents
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CCL22 protein, human
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Chemokine CCL22
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Chemokines, CC
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HIV Core Protein p24
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Receptors, Chemokine
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Receptors, HIV
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Calcium