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Enrichment of bifidobacteria from human gut contents by oligofructose using continuous culture

FEMS Microbiol Lett. 1994 May 1;118(1-2):121-7. doi: 10.1111/j.1574-6968.1994.tb06813.x.

Abstract

Chemostat cultures of human faecal bacteria were used to determine the bifidogenic effect of oligofructose, a fermentable carbohydrate found in a number of plants. In single stage continuous culture, oligofructose preferentially enriched for bifidobacteria, in comparison to sucrose and inulin. This stimulatory effect was enhanced at a high dilution rate, high substrate concentration and low pH. These parameters are likely to approximate to those that occur in the proximal colon. Studies with a three-stage continuous culture model of the large intestine confirmed the bifidogenic effect of oligofructose. These in vitro data indicate that an increase in the concentration of fructose-based oligosaccharides in the diet may alter the balance of the gut microflora towards bifidobacteria, a purported health-promoting genus.

MeSH terms

  • Anaerobiosis
  • Bacteria, Aerobic / growth & development
  • Bacteria, Anaerobic / growth & development
  • Bifidobacterium / growth & development*
  • Bifidobacterium / metabolism
  • Colon / microbiology*
  • Colony Count, Microbial
  • Culture Media
  • Fatty Acids / biosynthesis
  • Feces / microbiology*
  • Fermentation
  • Fructose / metabolism*
  • Glucose
  • Humans
  • Hydrogen-Ion Concentration
  • Inulin
  • Oligosaccharides / metabolism*

Substances

  • Culture Media
  • Fatty Acids
  • Oligosaccharides
  • Fructose
  • Inulin
  • Glucose