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Genetic Polymorphisms of GP1BA, PEAR1, and PAI-1 may be Associated with Serum sIgE and Blood Eosinophil Levels in Chinese Patients with Allergic Diseases

Endocr Metab Immune Disord Drug Targets. 2024;24(10):1215-1223. doi: 10.2174/0118715303285101240118062549.

Abstract

Background: It has been suggested that genetic factors may be substantially linked to allergy disorders.

Objective: This study aims to investigate the relationship between the serum specific Immunoglobulin E (sIgE), blood eosinophil, and the polymorphisms of glycoprotein Ib alpha gene (GP1BA) rs6065, platelet endothelial aggregation receptor 1 gene (PEAR1) rs12041331, and plasminogen activator inhibitor 1 gene (PAI-1) rs1799762.

Methods: From the Peking Union Medical College Hospital, this study enrolled 60 healthy participants and 283 participants with allergic diseases. TaqMan-minor groove binder (MGB) quantitative polymerase chain reaction (qPCR) was used to examine the gene polymorphisms in each group.

Results: The TaqMan-MGB qPCR results were completely consistent with the DNA sequencing results, according to other studies in this medical center (Kappa =1, p <0.001). The GP1BA rs6065, PEAR1 rs12041331, and PAI-1 rs1799762 polymorphisms did not show different distribution between allergy patients and healthy individuals. Concerning allergy patients, the CT (n=33) genotype of GP1BA rs6065 had higher blood eosinophil level than the CC (n=250) genotype (0.59, IQR 0.32-0.72 vs 0.31, IQR 0.15-0.61, *109/L, p =0.005). The serum sIgE of AA (n=46) genotype of PEAR1 rs12041331 was lower (median 3.7, interquartile quartiles (IQR) 0.2-16.8, kU/L) than the GA (n=136) and GG (n=101) genotypes (GA median 16.3, IQR 3.1-46.3, kU/L, p = 0.002; GG median 12.9, IQR 3.0-46.9, kU/L, p =0.003). The GA genotypes of PEAR1 rs12041331were with higher blood eosinophil levels (median 0.42, IQR 0.17-0.74 *109/L) than the AA genotype (median 0.25, IQR 0.15-0.41*109/L, p =0.012). The sIgE of the 5G5G (n=44) genotype of PAI-1 rs1799762 was lower (median 5.0, IQR 0.1-22.8, kU/L) than the 4G5G (n=144) (median 17.3, IQR 3.7-46.0, kU/L, p = 0.012).

Conclusion: The GP1BA rs6065, PEAR1 rs12041331, and PAI-1 rs1799762 polymorphisms may be associated with the genetic susceptibility of serum sIgE or blood eosinophil in Chinese allergic disease patients.

Keywords: Allergy and immunology; eosinophil; gene polymorphism; glycoprotein Ib alpha gene (GP1BA); plasminogen activator inhibitor 1 gene (PAI-1).; platelet endothelial aggregation receptor 1 (PEAR1); specific immunoglobulin E (sIgE).

MeSH terms

  • Adolescent
  • Adult
  • Case-Control Studies
  • China / epidemiology
  • East Asian People / genetics
  • Eosinophils*
  • Female
  • Genetic Association Studies
  • Genetic Predisposition to Disease
  • Humans
  • Hypersensitivity* / blood
  • Hypersensitivity* / genetics
  • Immunoglobulin E* / blood
  • Male
  • Middle Aged
  • Plasminogen Activator Inhibitor 1* / blood
  • Plasminogen Activator Inhibitor 1* / genetics
  • Platelet Glycoprotein GPIb-IX Complex* / genetics
  • Polymorphism, Single Nucleotide
  • Receptors, Cell Surface* / blood
  • Receptors, Cell Surface* / genetics
  • Young Adult

Substances

  • Immunoglobulin E
  • PEAR1 protein, human
  • Plasminogen Activator Inhibitor 1
  • Platelet Glycoprotein GPIb-IX Complex
  • Receptors, Cell Surface
  • SERPINE1 protein, human
  • adhesion receptor