Ultra-widefield and high-speed spiral laser scanning OR-PAM: System development and characterization

J Biophotonics. 2023 Jul;16(7):e202200383. doi: 10.1002/jbio.202200383. Epub 2023 Apr 11.

Authors

Mohsin Zafar^{1

2}, Rayyan Manwar¹, Laura S McGuire³, Fady T Charbel³, Kamran Avanaki^{1

4}

Affiliations

¹ The Richard and Loan Hill, Department of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois, USA.
² Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, USA.
³ Department of Neurological Surgery, University of Illinois at Chicago - College of Medicine, Chicago, Illinois, USA.
⁴ Department of Dermatology, University of Illinois at Chicago, Chicago, Illinois, USA.

PMID: 36998211
DOI: 10.1002/jbio.202200383

Abstract

Photoacoustic microscopy (PAM) is a high-resolution imaging modality that has been mainly implemented with small field of view applications. Here, we developed a fast PAM system that utilizes a unique spiral laser scanning mechanism and a wide acoustic detection unit. The developed system can image an area of 12.5 cm² in 6.4 s. The system has been characterized using highly detailed phantoms. Finally, the imaging capabilities of the system were further demonstrated by imaging a sheep brain ex vivo and a rat brain in vivo.

Keywords: fast imaging; large field of view; photoacoustic microscopy; spiral scanning.

Publication types

Letter
Research Support, N.I.H., Extramural

MeSH terms

Animals
Lasers
Light
Microscopy* / methods
Phantoms, Imaging
Photoacoustic Techniques* / methods
Rats
Sheep
Spectrum Analysis

Abstract

Publication types

MeSH terms

Grants and funding