Linc-ROR facilitates progression and angiogenesis of hepatocellular carcinoma by modulating DEPDC1 expression

Cell Death Dis. 2021 Nov 5;12(11):1047. doi: 10.1038/s41419-021-04303-5.

Authors

Chuan Tian¹, Mubalake Abudoureyimu¹, Xinrong Lin¹, Xiaoyuan Chu¹, Rui Wang²

Affiliations

¹ Department of Medical Oncology, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
² Department of Medical Oncology, Affiliated Jinling Hospital, Medical School of Nanjing University, Nanjing, China. wangrui218@163.com.

Abstract

Linc-ROR have been well-demonstrated to play important roles in cancer progression and angiogenesis. However, the underlying oncogenic mechanism of Linc-ROR in hepatocellular carcinoma is poorly understood. In this study, we demonstrate that Linc-ROR plays an oncogenic role in part through its positive regulation of DEPDC1 expression. Mechanistically, Linc-ROR acts as competing endogenous RNA to stabilize DEPDC1 mRNA and regulates DEPDC1 mRNA stability by binding HNRNPK. Thus, these findings suggest that function of Linc-ROR-mediated DEPDC1 could predispose hepatocellular carcinoma patients to progression and angiogenesis, and may serve as a potential target for anticancer therapies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Carcinogenesis / genetics
Carcinogenesis / pathology
Carcinoma, Hepatocellular / blood supply*
Carcinoma, Hepatocellular / genetics*
Carcinoma, Hepatocellular / pathology
Cell Line, Tumor
Cell Proliferation / genetics
Disease Progression*
Epithelial-Mesenchymal Transition / genetics
Female
GTPase-Activating Proteins / genetics*
GTPase-Activating Proteins / metabolism
Gene Expression Regulation, Neoplastic*
Heterogeneous-Nuclear Ribonucleoprotein K / genetics
Heterogeneous-Nuclear Ribonucleoprotein K / metabolism
Human Umbilical Vein Endothelial Cells / metabolism
Humans
Liver Neoplasms / blood supply
Liver Neoplasms / genetics*
Liver Neoplasms / pathology
Male
Mice
Mice, Inbred BALB C
Mice, Nude
MicroRNAs / genetics
MicroRNAs / metabolism
Middle Aged
Neoplasm Invasiveness
Neoplasm Proteins / genetics*
Neoplasm Proteins / metabolism
Neovascularization, Pathologic / genetics*
Protein Binding
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Up-Regulation / genetics

Substances

DEPDC1 protein, human
GTPase-Activating Proteins
Heterogeneous-Nuclear Ribonucleoprotein K
Linc-RNA-RoR, human
MIRN130 microRNA, human
MicroRNAs
Neoplasm Proteins
RNA, Long Noncoding
RNA, Messenger
HNRNPK protein, human