The effects of undulating seasonal temperature on the performance and microbial community characteristics of simultaneous anammox and denitrification (SAD) process

The effects of undulating seasonal temperature change (USTC) (10.1 °C-31.8 °C) on the N and carbon removal efficiency of simultaneous anammox and denitrification (SAD) were investigated, and the recovery performance of SAD was simulated. Results showed that 15 °C was the critical temperature of SAD for N and carbon removal under USTC from summer to winter. The removal efficiency of NH₄⁺-N was improved in the final stage after temperature rise, but still lower than that in summer after long-term low temperature inhibition. The contribution of anammox to N removal was more than denitrification. The abundance of anammox bacteria (AnAOB) in SAD reactor was 8.8%-11.7% from summer to autumn. Candidatus Kuenenia replaced Candidatus Brocadia as the main AnAOB gradually. Finally, AnAOB abundance increased from 4.2% to 6.6% after recovery, and the abundance of denitrifying bacteria (DB) became the highest, which mainly includes Thauera and Hydrogenophaga.