Purpose: To study the effect of micro ribonucleic acid (miR)-26a on the proliferation and apoptosis of uveal melanoma (UM) cell lines, and to explore the potential signaling pathway.
Methods: UM SP6.5 cells were used in this study, and were transfected with miR-26a mimic (miR-26a mimic group) and miR-26a small-interfering RNA (siRNA) (miR-26a siRNA group) using Lipofectamine 2000 transfection reagent, with miR-26a negative control (NC) as the blank controls (miR-26a NC group). The level of miR-26a in SP6.5 cells was detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and the effects of miR-26a on the viability, proliferation and apoptosis of SP6.5 cells were detected via cell counting kit-8 (CCK-8) assay and colony formation assay.
Results: Compared with those in the miR-26a NC group, SP6.5 cells in the miR-26a siRNA group had significantly enhanced viability and proliferation, a significantly decreased apoptosis rate, reduced mRNA and protein levels of p53, and obviously increased mRNA and protein levels of MDM2. Moreover, in comparison with those in the miR-26a NC group, SP6.5 cells in.the miR-26a mimic group had evidently weakened viability and proliferation, an evidently higher apoptosis rate, increased mRNA and protein levels of p53, and markedly lower mRNA and protein levels of MDM2.
Conclusions: Highly expressed miR-26a can inhibit the proliferation and promote apoptosis of SP6.5 cells, whose potential mechanism may be related to the regulation on the p53/MDM2 pathway.