Pathogenic and antimicrobial resistance genes in Streptococcus oralis strains revealed by comparative genome analysis

Genomics. 2020 Sep;112(5):3783-3793. doi: 10.1016/j.ygeno.2020.04.014. Epub 2020 Apr 22.

Authors

Jiannan Zhou¹, Tianyong Sun¹, Wenyan Kang¹, Di Tang¹, Qiang Feng²

Affiliations

¹ Department of Human Microbiome, School and Hospital of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No. 44-1 Wenhua Road West, 250012 Jinan, Shandong, China.
² Department of Human Microbiome, School and Hospital of Stomatology, Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No. 44-1 Wenhua Road West, 250012 Jinan, Shandong, China; State Key Laboratory of Microbial Technology, Shandong University,266237 Qingdao, Shandong, China; NHC Key Laboratory of Otorhinolaryngology (Shandong University), Jinan, Shandong, China. Electronic address: fengqiang@sdu.edu.cn.

PMID: 32334114
DOI: 10.1016/j.ygeno.2020.04.014

Abstract

Streptococcus oralis is an early colonizer bacterium in dental plaques and is considered a potential pathogen of infective endocarditis (IE) disease. In this study, we built a complete genome map of Streptococcus oralis strain SOT, Streptococcus oralis strain SOD and Streptococcus infantis strain SO and performed comparative genomic analysis among these three strains. The results showed that there are five genomic islands (GIs) in strain SOT and one CRISPR in strain SOD. Each genome harbors various pathogenic genes related to diseases and drug resistance, while the antibiotic resistance genes in strains SOT and SOD were quite similar but different from those in strain SO. In addition, we identified 17 main virulence factors and capsule-related genes in three strains. These results suggest the pathogenic potential of Streptococcus strains, which lay a foundation for the prevention and treatment of a Streptococcus oralis infection.

Keywords: Comparative genome; Drug resistance; Fine map; Streptococcus oralis; Virulence gene.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Comparative Genomic Hybridization*
Drug Resistance, Bacterial / genetics*
Genes, Bacterial*
RNA, Ribosomal, 16S / genetics
Streptococcus oralis / drug effects
Streptococcus oralis / genetics*
Virulence Factors / genetics

Substances

Anti-Bacterial Agents
RNA, Ribosomal, 16S
Virulence Factors