Self-assembled CpG oligodeoxynucleotides conjugated hollow gold nanospheres to enhance cancer-associated immunostimulation

Colloids Surf B Biointerfaces. 2019 Mar 1:175:248-255. doi: 10.1016/j.colsurfb.2018.12.001. Epub 2018 Dec 3.

Authors

Jing Luo¹, Yang Cheng¹, Xiao-Yan He², Yi Liu³, Na Peng¹, Zhi-Wei Gong¹, Kui Wu¹, Tao Zou⁴

Affiliations

¹ Key Laboratory of Coal Conversion & New Carbon Materials of Hubei Province, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, PR China.
² Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
³ Key Laboratory of Coal Conversion & New Carbon Materials of Hubei Province, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, PR China; State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine (MOE), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
⁴ Key Laboratory of Coal Conversion & New Carbon Materials of Hubei Province, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, PR China. Electronic address: taozou@wust.edu.cn.

PMID: 30540972
DOI: 10.1016/j.colsurfb.2018.12.001

Abstract

To realize efficiently cellular uptake and enhance the immunostimulation, thiolated cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) were self-assembled on hollow gold nanospheres (HGNs) to form CpG-HGNs. The cellular uptake of CpG-HGNs in immune cells was studied in RAW 264.7 cells. Due to the enhanced delivery efficiency, CpG-HGNs exhibited a higher immune stimulatory activity compared with CpG ODNs, resulting in a dramatically enhanced secretion of proinflammatory cytokines. In addition, CpG-HGNs showed low cytotoxicity in RAW 264.7 cells. CpG-HGNs could potentially realize synergistic photothermal therapy and immunotherapy in vivo.

Keywords: CpG oligodeoxynucleotide; Hollow gold nanosphere; Immunostimulation; Photothermal; Self-assembled.

MeSH terms

Animals
Cell Survival / drug effects
Cell Survival / radiation effects
Cytokines / metabolism
Gold / chemistry*
Humans
Inflammation Mediators / metabolism
Lasers
Mice
Microscopy, Electron, Transmission
Nanospheres / administration & dosage
Nanospheres / chemistry*
Nanospheres / ultrastructure
Neoplasms / metabolism
Neoplasms / therapy
Oligodeoxyribonucleotides / chemistry*
RAW 264.7 Cells
Temperature

Substances

Cytokines
Inflammation Mediators
Oligodeoxyribonucleotides
Gold