Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues

Anal Chem. 2017 Aug 15;89(16):8496-8500. doi: 10.1021/acs.analchem.7b02059. Epub 2017 Jul 24.

Authors

Jun Li¹, Chang Su Lim², Gyoungmi Kim¹, Hwan Myung Kim², Juyoung Yoon¹

Affiliations

¹ Department of Chemistry and Nano Science, Ewha Womans University , Seoul 120-750, Korea.
² Department of Chemistry and Energy Systems Research, Ajou University , Suwon 443-749, Korea.

PMID: 28695725
DOI: 10.1021/acs.analchem.7b02059

Abstract

A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO^-) detection was designed and synthesized. The probe exhibits good selectivity and sensitivity for ONOO^- in phosphate-buffered saline solution with a low detection limit (3.5 × 10^-8 M). Furthermore, the probe displays good performance in detecting endogenous ONOO^-, not only in RAW 264.7 cells but also in rat hippocampal tissue, with a high two-photon cross-section value (δ ≈ 100 GM) at a deep depth of 120 μm.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Hippocampus / chemistry*
Hippocampus / cytology
Hippocampus / metabolism
Mice
Microscopy, Fluorescence
Molecular Structure
Peroxynitrous Acid / analysis*
Photons*
RAW 264.7 Cells
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism
Solutions

Substances

Fluorescent Dyes
Reactive Oxygen Species
Solutions
Peroxynitrous Acid