Abstract
Galectin-3 (gal-3), a pleiotrophic protein, is an important regulator of tumor metastasis, which like beta-catenin shuttles between the nucleus and the cytosol in a phosphorylation-dependent manner. We report herein that beta-catenin stimulation of cyclin D1 and c-myc expression is gal-3 dependent. Gal-3 binds to beta-catenin/Tcf complex, colocalizes with beta-catenin in the nucleus, and induces the transcriptional activity of Tcf-4 as determined by the TOP/FOPFLASH reporter system. We have identified the beta-catenin-gal-3-binding sequences, which are in the NH2 and COOH termini of the proteins encompassing amino acid residues 1 to 131 and 143 to 250, respectively. These data indicate that gal-3 is a novel binding partner for beta-catenin involved in the regulation of Wnt/beta-catenin signaling pathway.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Breast Neoplasms / genetics
-
Breast Neoplasms / metabolism
-
Cell Line, Tumor
-
Cytoskeletal Proteins / genetics
-
Cytoskeletal Proteins / metabolism*
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
Galectin 3 / genetics
-
Galectin 3 / metabolism*
-
Gene Expression Regulation, Neoplastic
-
Genes, bcl-1
-
HCT116 Cells
-
HT29 Cells
-
Humans
-
Plasmids / genetics
-
Promoter Regions, Genetic
-
Protein Binding
-
Protein Structure, Tertiary
-
TCF Transcription Factors
-
Trans-Activators / genetics
-
Trans-Activators / metabolism*
-
Transcription Factor 7-Like 2 Protein
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
-
Transcriptional Activation
-
Transfection
-
Up-Regulation
-
beta Catenin
Substances
-
CTNNB1 protein, human
-
Cytoskeletal Proteins
-
DNA-Binding Proteins
-
Galectin 3
-
TCF Transcription Factors
-
TCF7L2 protein, human
-
Trans-Activators
-
Transcription Factor 7-Like 2 Protein
-
Transcription Factors
-
beta Catenin