Rotenone (Synonyms: 鱼藤酮)

目录号: HY-B1756 纯度: 99.74%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

Rotenone 是线粒体电子传递链复合物 I 抑制剂。 Rotenone 通过促进线粒体活性氧的产生来诱导细胞凋亡。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Rotenone Chemical Structure

CAS No. : 83-79-4

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥500	In-stock
500 mg	￥400	In-stock
1 g	￥550	In-stock
5 g	￥1100	In-stock
10 g		询价
50 g		询价

or 大包装询价

* Please select Quantity before adding items.

Other Forms of Rotenone:

Rotenone (Standard) 询价

MCE 顾客使用本产品发表的 80 篇科研文献

: Rotenone purchased from MCE. Usage Cited in: Mediat Inflamm. 2020 Aug 17;2020:3934769. [Abstract]; Western blot analysis of NLRP3, IL-1β, IL-18, and caspase-1 p10 expression in renal tubular epithelial cells stimulated with Rotenone. Rotenone induces NLRP3 inflammasome activation.

Rotenone 相关产品

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•同靶点产品:

•同靶点蛋白产品:

生物活性
纯度 & 产品资料
参考文献

生物活性

Rotenone is a mitochondrial electron transport chain complex I inhibitor. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
697	IC₅₀	0.3 μM Compound: 8	Cytotoxicity against human 697 cells after 48 hrs by MTS assay Cytotoxicity against human 697 cells after 48 hrs by MTS assay	[PMID: 23895019]
697	IC₅₀	0.3 μM Compound: 8	Cytotoxicity against human 697 cells after 72 hrs by MTS assay Cytotoxicity against human 697 cells after 72 hrs by MTS assay	[PMID: 23895019]
A549	IC₅₀	0.04 μg/mL Compound: 3	Cytotoxicity against human A549 cells after 3 days by SRB assay Cytotoxicity against human A549 cells after 3 days by SRB assay	[PMID: 16562843]
A549	IC₅₀	0.901 μg/mL Compound: 1	Cytotoxicity against human A549 cells by SRB assay Cytotoxicity against human A549 cells by SRB assay	[PMID: 22204911]
A549	ED₅₀	26 μM Compound: rotenone	Antitumor activity against human A549 cells after 24 hrs by MTT assay Antitumor activity against human A549 cells after 24 hrs by MTT assay	[PMID: 17482824]
BC1 cell line	ED₅₀	0.039 μg/mL Compound: 1	Cytotoxicity against human BC1 cells after 72 hrs Cytotoxicity against human BC1 cells after 72 hrs	[PMID: 2614425]
Col2	ED₅₀	0.15 μg/mL Compound: 1	Cytotoxicity against human Col2 cells after 72 hrs Cytotoxicity against human Col2 cells after 72 hrs	[PMID: 2614425]
DU-145	IC₅₀	0.465 μg/mL Compound: 1	Cytotoxicity against human DU145 cells by SRB assay Cytotoxicity against human DU145 cells by SRB assay	[PMID: 22204911]
HCT-116	IC₅₀	0.045 ug Compound: 5	Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting	[PMID: 21452840]
HeLa	IC₅₀	78 μM Compound: Rotenone	Cytotoxicity against human HeLa cells after 24 hrs by luminometry Cytotoxicity against human HeLa cells after 24 hrs by luminometry	[PMID: 21741837]
HepG2	IC₅₀	1.52 μM Compound: Rotenone	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 30613327]
HepG2	ED₅₀	15 μM Compound: rotenone	Antitumor activity against human HepG2 cells after 24 hrs by MTT assay Antitumor activity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 17482824]
HT-1080	ED₅₀	0.047 μg/mL Compound: 1	Cytotoxicity against human HT1080 cells after 48 hrs Cytotoxicity against human HT1080 cells after 48 hrs	[PMID: 2614425]
HT-29	IC₅₀	0.3 μM Compound: 8	Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay	[PMID: 23895019]
HT-29	ED₅₀	202 μM Compound: rotenone	Antitumor activity against human HT29 cells after 24 hrs by MTT assay Antitumor activity against human HT29 cells after 24 hrs by MTT assay	[PMID: 17482824]
KB	ED₅₀	0.067 μg/mL Compound: 1	Cytotoxicity against human KB cells after 72 hrs Cytotoxicity against human KB cells after 72 hrs	[PMID: 2614425]
KB	IC₅₀	0.513 μg/mL Compound: 1	Cytotoxicity against human KB cells by SRB assay Cytotoxicity against human KB cells by SRB assay	[PMID: 22204911]
L5178Y	IC₅₀	0.3 μM Compound: 30	Cytotoxicity against mouse L5178Y cells by MTT assay Cytotoxicity against mouse L5178Y cells by MTT assay	[PMID: 28075580]
MCF7	IC₅₀	0.008 μg/mL Compound: 3	Cytotoxicity against human MCF7 cells after 3 days by SRB assay Cytotoxicity against human MCF7 cells after 3 days by SRB assay	[PMID: 16562843]
MCF7	ED₅₀	161 μM Compound: rotenone	Antitumor activity against human MCF7 cells after 24 hrs by MTT assay Antitumor activity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 17482824]
MES-SA	ED₅₀	147 μM Compound: rotenone	Antitumor activity against human MES-SA cells after 24 hrs by MTT assay Antitumor activity against human MES-SA cells after 24 hrs by MTT assay	[PMID: 17482824]
MES-SA/Dx5	ED₅₀	214 μM Compound: rotenone	Antitumor activity against doxorubucin-resistant human MES-SA/Dx5 cells by MTT assay after 24 hrs Antitumor activity against doxorubucin-resistant human MES-SA/Dx5 cells by MTT assay after 24 hrs	[PMID: 17482824]
P388	ED₅₀	0.005 μg/mL Compound: 1	Cytotoxicity against mouse P388 cells after 48 hrs Cytotoxicity against mouse P388 cells after 48 hrs	[PMID: 2614425]
Raji	IC₅₀	1.1 μM Compound: 8	Cytotoxicity against human Raji cells after 72 hrs by MTS assay Cytotoxicity against human Raji cells after 72 hrs by MTS assay	[PMID: 23895019]
Raji	IC₅₀	4.2 μM Compound: 8	Cytotoxicity against human Raji cells after 48 hrs by MTS assay Cytotoxicity against human Raji cells after 48 hrs by MTS assay	[PMID: 23895019]
SH-SY5Y	IC₅₀	74.1 μM Compound: Rotenone	Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 27689727]
SK-MEL-2	ED₅₀	0.092 μg/mL Compound: 1	Cytotoxicity against human SK-MEL-2 cells after 72 hrs Cytotoxicity against human SK-MEL-2 cells after 72 hrs	[PMID: 2614425]
U-937	IC₅₀	0.07 μM Compound: 3	Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	0.2 μM Compound: 3	Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	13 μM Compound: 3	Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay	[PMID: 17158054]

体外研究
(In Vitro)

丝裂原活化蛋白激酶 (MAPK)、Toll 样受体、Wnt 和 Ras 信号通路密切参与 Rotenone 对 ENS 的影响^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Rotenone 可用于构建帕金森模型。

诱导帕金森模型^[6]

致病原理

黑质多巴胺能 (DA) 神经元细胞丢失，是帕金森的常见特征。Rotenone 能够诱导 DA 神经元细胞毒性，导致小鼠黑质运动缺陷和 DA 神经元细胞的丢失。

具体造模方法：

小鼠：雄性 • C57BL/6J 小鼠 • 8 周龄 • 20-25 g
给药方式：30 mg/kg, 12 mL/kg • 口服 • 每天一次，连续 28 天

Note

对照组用 0.5% Carboxylmethylcellulose (CMC) 处理。

造模成功指标

小鼠运动障碍：运动迟缓/运动能力不足。

相关产品： /

拮抗产品： /

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

394.42

Formula

C₂₃H₂₂O₆

CAS 号

83-79-4

性状

固体

颜色

White to light yellow

中文名称

鱼藤酮

结构分类

Flavonoids

初始来源

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen

*In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (126.77 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.5354 mL	12.6768 mL	25.3537 mL
5 mM	0.5071 mL	2.5354 mL	5.0707 mL
10 mM	0.2535 mL	1.2677 mL	2.5354 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (stored under nitrogen)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.34 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.34 mM); 悬浊液; 超声助溶

此方案可获得 2.5 mg/mL的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.34 mM); 澄清溶液

此方案可获得 ≥ 2.5 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
方案四

请依序添加每种溶剂： 5% DMSO 95% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.34 mM); 悬浊液; 超声助溶

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： 0.5% CMC-Na/saline water
Solubility: 25 mg/mL (63.38 mM); 悬浊液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

2"> +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

*In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

50.0"> 您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.74%

选择批次：

Data Sheet (650 KB) SDS (623 KB)

COA (291 KB) HNMR (263 KB) RP-HPLC (226 KB) LCMS (93 KB) OR (256 KB) 元素分析报告 (214 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Khadrawy YA, et al. Cerebellar neurochemical and histopathological changes in rat model of Parkinson's disease induced by intrastriatal injection of rotenone. Gen Physiol Biophys. 2016 Nov 30. [Content Brief]

[2]. Guan Q, et al. RNA-Seq Expression Analysis of Enteric Neuron Cells with Rotenone Treatment and Prediction of Regulated Pathways. Neurochem Res. 2016 Nov 30. [Content Brief]

[3]. Kishore Kumar SN, et al. Morinda citrifolia mitigates rotenone-induced striatal neuronal loss in male Sprague-Dawley rats by preventing mitochondrial pathway of intrinsic apoptosis. Redox Rep. 2016 Nov 24:1-12. [Content Brief]

[4]. Zhang ZN, et al. Subcutaneous rotenone rat model of Parkinson's disease: dose exploration study. Brain Res. 2016 Nov 19. pii: S0006-8993(16)30776-4. [Content Brief]

[5]. Li N, et al. Mitochondrial complex I inhibitor rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production. J Biol Chem. 2003 Mar 7;278(10):8516-25. [Content Brief]

Rotenone 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5354 mL	12.6768 mL	25.3537 mL	63.3842 mL
	5 mM	0.5071 mL	2.5354 mL	5.0707 mL	12.6768 mL
	10 mM	0.2535 mL	1.2677 mL	2.5354 mL	6.3384 mL
	15 mM	0.1690 mL	0.8451 mL	1.6902 mL	4.2256 mL
	20 mM	0.1268 mL	0.6338 mL	1.2677 mL	3.1692 mL
	25 mM	0.1014 mL	0.5071 mL	1.0141 mL	2.5354 mL
	30 mM	0.0845 mL	0.4226 mL	0.8451 mL	2.1128 mL
	40 mM	0.0634 mL	0.3169 mL	0.6338 mL	1.5846 mL
	50 mM	0.0507 mL	0.2535 mL	0.5071 mL	1.2677 mL
	60 mM	0.0423 mL	0.2113 mL	0.4226 mL	1.0564 mL
	80 mM	0.0317 mL	0.1585 mL	0.3169 mL	0.7923 mL
	100 mM	0.0254 mL	0.1268 mL	0.2535 mL	0.6338 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rotenone83-79-4鱼藤酮Mitochondrial MetabolismAutophagyApoptosisInhibitorinhibitorinhibit

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Rotenone (Synonyms: 鱼藤酮)

Other Forms of Rotenone:

MCE 顾客使用本产品发表的 80 篇科研文献

Rotenone 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

生物活性

纯度 & 产品资料

参考文献

摩尔计算器

稀释计算器

Rotenone 相关分类

完整储备液配制表

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Rotenone (Synonyms: 鱼藤酮)

Other Forms of Rotenone:

Rotenone 相关抗体

MCE 顾客使用本产品发表的 80 篇科研文献

Rotenone 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

生物活性

纯度 & 产品资料

参考文献

Rotenone 相关抗体:

摩尔计算器

稀释计算器

Rotenone 相关分类

完整储备液配制表

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您最近查看的产品:

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